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1.
Endocrine ; 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32562184

RESUMO

BACKGROUND: Both thyroid-stimulating immunoglobulins immunoassay (TSI IA) and thyrotrophin receptor antibody immunoassay (TRAb IA) are commonly used for the diagnosis of Graves' disease (GD). The aim of the present study was to compare the clinical diagnostic performance of these two methods. METHODS: Sera were obtained from 1103 subjects presenting a variety of clinical conditions from three centers: 100 subjects with untreated GD, 200 with treated GD, 62 with autoimmune thyroid disease(AIT), 216 with other thyroid diseases (OTHER-T), 214 with non-thyroid autoimmune diseases (NTAD), 191 with other diseases (OD), and 120 healthy subjects (HS). Both TSI and TRAb IAs were performed for all 1013 serum samples. Bioassay was performed for 86 samples whose TSI results were inconsistent the TRAb assay results. RESULTS: Comparing untreated GD patients with the control groups (AIT, NTAD, OTHER-T, OD, and HS) resulted in an area under the curve (AUC) of 0.992 for the TSI IA and 0.989 for the TRAb IA with no statistically significant difference observed between these AUC values (P = 0.2733). The best TSI CDP (clinical decision point) value was 0.42 IU/L. The differences in sensitivity (100% vs. 95%, P = 0.7991) and specificity (97.1% vs. 97.6%, P = 0.9426) between the TSI and TRAb IA were not statistically significant. TSI IA had a higher agreement with the TSI bioassay than TRAb IA. CONCLUSION: The clinical diagnostic performance of the TSI IA for diagnosing Graves' disease was very similar to that of the TRAb IA. TSI IA can be used to diagnose GD in the Chinese.

2.
Mol Pain ; 16: 1744806920930855, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32498644

RESUMO

Trigeminal neuralgia is a common neuropathic pain in the head and face. The pathogenesis of trigeminal neuralgia is complex, and so far, the pathogenesis of trigeminal neuralgia involving peripheral and central nervous inflammation theory has not been explained clearly. The loss of dopamine neurons in striatum may play an important role in the development of trigeminal nerve, but the reason is not clear. C-Abl is a nonreceptor tyrosine kinase, which can be activated abnormally in the environment of neuroinflammation and cause neuron death. We found that in the rat model of infraorbital nerve ligation trigeminal neuralgia, the pain threshold decreased, the expression of c-Abl increased significantly, the downstream activation product p38 was also activated abnormally and the loss of dopamine neurons in striatum increased. When treated with imatinib mesylate (STI571), a specific c-Abl family kinase inhibitor, the p38 expression was decreased and the loss of dopaminergic neurons was reduced. The mechanical pain threshold of rats was also improved. In conclusion, c-abl-p38 signaling pathway may play an important role in the pathogenesis of trigeminal neuralgia, and it is one of the potential targets for the treatment of trigeminal neuralgia.

3.
Nephrology (Carlton) ; 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32542819

RESUMO

AIMS: Gitelman syndrome (GS) is a rare inherited salt-losing renal tubulopathy. Data on clinical features and the pregnancy outcome for female GS patients in a large cohort are lacking. The study was aimed to explore the phenotype and pregnant issue for female GS patients. METHODS: GS cases from the National Rare Diseases Registry System of China (NRSC) were collected, and detailed clinical, laboratory and genetic data were analyzed. Articles on pregnancy in GS were also systemically reviewed. RESULTS: A total of 101 GS patients were included, among them 42.6% was female and 79.2% showed hypomagnesemia. A lower proportion of female patients presented before age 18 years, with less frequently reported polyuria, higher serum potassium, and less urine sodium and chloride excretions. There was no gender difference in the sodium-chloride cotransporter (NCC) dysfunction evaluated by hydrochlorothiazide test. Twelve of the 43 female GS patients delivered after disease symptom onset, and their pregnancies were generally uneventful. As a group, pregnant GS patients had lower potassium levels in the first-trimester (P = 0.002) requiring higher potassium supplementation. After delivery, serum potassium (P = 0.02) and magnesium (P = 0.03) increased significantly. Both cesarean section and vaginal delivery were safe. CONCLUSION: Female GS patients may have a less severe phenotype with generally favorable outcomes of pregnancy. Intensive monitoring and increased potassium supplementation are necessary during pregnancy, especially in the first-trimester. This article is protected by copyright. All rights reserved.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32386488

RESUMO

BACKGROUND: Lung cancer is the leading cause of cancer-associated mortality in the world. Traditional cancer therapies prolong life expectancy of patients but often suffer from adverse reactions. Photodynamic therapy (PDT) has been recommended as a treatment option for lung cancer in several countries, due to its non-invasive procedures, high selectivity and weak side effects. OBJECTIVE: We have designed and synthesized a biotin receptor-targeted silicon phthalocyanine (IV) (compound 1) which showed good therapeutic effect on biotin receptor-positive tumors. Since the overexpression of biotin receptor (BR) is also present in human lung cancer cells (A549), we explored the therapeutic properties of compound 1 on A549 xenograft tumor models. METHOD: The selectivity of compound 1 toward A549 cells was studied with fluorescence microscope and IVIS Spectrum Imaging System. The cytotoxicity was measured using MTT assay. In vivo anti-tumor activity was investigated on the nude mice bearing A549 xenografts. RESULTS: In vitro assays proved that compound 1 could selectively accumulate in A549 cells via the BR-mediated internalization. In vivo imaging and distribution experiments showed that compound 1 could selectively accumulate in tumor tissues of tumor-bearing mice. After 16 days of the treatment, the volumes of tumor in PDT group were obviously smaller than that in other groups. CONCLUSION: This study demonstrates that compound 1 is a promising photosensitizer and has broad application prospects in clinical PDT of lung cancers.

5.
Nanoscale ; 2020 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-32388544

RESUMO

Nickel-iron (Ni-Fe) compounds have received ever-increasing interest because of their high performance for the oxygen evolution reaction (OER). However, the structure and composition of the active phase of Ni-Fe compounds are not yet understood. Rational design of Ni-Fe compounds with proper composition and exposed active sites is highly desirable to further improve their performance. Here we reported the synthesis of different Ni-Fe compounds by incorporating Fe into the Ni(OH)2 lattice with controlled concentrations and obtained several products including Ni-Fe layered double hydroxides (LDHs) with different amounts of Fe and NiFe2O4. In addition, ß-Ni(OH)2 without Fe and α-Fe2O3 without Ni have also been synthesized for comparison. Among these four Ni/Fe based compounds, we found that the two-dimensional (2D) Ni0.8Fe0.2-LDH nanosheets with a hexagonal shape manifest a highest OER activity with a lowest overpotential of 235 mV at 10 mA cm-2, a smallest Tafel slope of 41 mV dec-1 and excellent stability over 24 h. In situ Raman, X-ray absorption spectroscopy and X-ray photoelectron spectroscopy show that the enhanced activity of Ni0.8Fe0.2-LDH is due to the increased oxidation state of Ni from Ni2+ to Ni3+. Our findings demonstrate that the composition of Ni-Fe compounds influences the oxidizing ability of Ni and consequently their catalytic performance. We also reveal a strong correlation between the structure and oxidizing power of the Ni in Ni-Fe compounds and their OER activity, indicating that the well-engineered structure and Ni-site electronics are the primary origins of their OER activity. This work not only provides a promising guideline for the design and synthesis of Ni/Fe based compounds, but also highlights the crucial role of each component in enhancing the activity of these electrocatalysts.

6.
Nanoscale ; 2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-32393931

RESUMO

Mid-infrared (MIR) represents a crucial spectral region for applications in spectroscopy, sensing, imaging, security and industry screening, owing to the strong characteristic vibrational transitions of many important molecules. However, the current MIR compatible materials are fragile, hazardous, and costly, which hampers the performance of MIR devices. Here, we developed a versatile transmittance-based Kramers-Kronig method and obtained the optical properties of graphene oxide in the MIR region, unveiling its application potentials as a novel MIR compatible material. As an example, we demonstrated free-standing graphene oxide MIR polarizers with large extinction ratio (∼20 dB) and controllable working wavelength up to 25 µm, by using the low-cost and flexible direct laser writing technique. Our transmittance-based KK method offers a versatile approach to obtain the optical properties of novel atomic-scale low-dimensional materials in the less developed MIR region and opens up opportunities in high performing functional MIR devices.

7.
Cancer Biol Ther ; : 1-10, 2020 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-32453965

RESUMO

BACKGROUND: Despite recent progress in screening survival-related genes, there have been few attempts to apply methods based on cancer stem cells (CSCs) for prognosis. We aimed to identify a CSC-based model to predict survival in colorectal cancer (CRC) patients. MATERIAL/METHODS: Differentially expressed genes between CRC and normal tissues and between CD133- and CD133+ cells were obtained from The Cancer Genome Atlas and Gene Expression Omnibus, and intersections were evaluated. Gene Ontology functional and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyzes were performed. STRING was used to investigate interactions between the encoded proteins and the Kaplan-Meier method to verify mRNAs associated with survival. A prognostic model based on CSCs was established via univariate and multivariate Cox regression. Receiver operating characteristic curve analysis was conducted to test the model's sensitivity and specificity. The KS test was applied to provide evidence for relationships between expression levels of nine mRNAs in our model and pathological stage. RESULTS: In total, 155 common differentially expressed mRNAs were identified, and nine (AOC1, UCN, MTUS1, CDC20, SNCB, MAT1A, TUBB2B, GABRA4 and ALPP) were screened after regression analyses to establish a predictive model for classifying patients into high- and low-risk groups with significantly different overall survival times, especially for stage II and IV patients. CONCLUSIONS: We developed a novel model that provides additional and powerful prognostic information beyond conventional clinicopathological factors for CRC survival prediction. It also provides new insight into the molecular mechanisms underlying the transition from normal tissues to CSCs and formation of tumor tissues.

8.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(2): 394-399, 2020 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-32319368

RESUMO

OBJECTIVE: To investigate the effects of sanchinoside R1 (SR1) on cell growth, invasion and migration of HL-60 cells, as well as survival of AML mice. METHODS: AML mouse models were established by intravenous injection of HL-60 cells. The SR1 of 10, 25 and 50 mg/kg was intraperitoneally injected into AML models once a day for 1 week. The survival rate of mice, tumor volume and weight were measured, and protein levels of Caspase-3 and VEGF were determined by immunohistochemistry. And protein levels of p-PI3K, PI3K, p-AKT and AKT were detected by Western blot. RESULTS: SR1 significantly inhibited the growth of tumors (P<0.01, r=-0.9994, -0.9952) and increased the survival rate of mice (P<0.01). SR1 significantly increased the protein level of apoptosis-related Caspase-3(P<0.01, r=0.9855), and decreased the protein level of migration-related protein VEGF (P<0.01, r=-0.9848). The protein levels of p-PI3K and p-AKT were down-regulated by SR1, Thus, the relative protein levels of p-PI3K/PI3K and p-AKT/AKT were significantly decreased (P<0.01, r=-0.9372, -0.9953). Above-mentioned effects showed a significant positive/negative correlation with the concentration of SR1. CONCLUSION: SR1 inhibits the growth, invasion and migration of tumor cells, and increas survival of mice through affecting expression of Caspase-3, VEGF, p-PI3K, and p-AKT.


Assuntos
Leucemia Mieloide Aguda , Animais , Apoptose , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Ginsenosídeos , Células HL-60 , Humanos , Camundongos , Fosfatidilinositol 3-Quinases , Proteínas Proto-Oncogênicas c-akt , Transdução de Sinais
9.
Ecotoxicol Environ Saf ; 196: 110476, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32278143

RESUMO

Several studies have demonstrated that PM2.5 inhalation is associated with an increased risk of cerebrovascular disease (CVD), in which inflammation plays an important role. The mechanisms of this disease are not fully understood to date. Long non-coding RNAs (lncRNAs) are involved in many pathophysiological processes, such as immune responses; however, their functions associated with inflammation are largely unexplored. High-throughput sequencing assay and obtained numerous lncRNAs that altered the expression in response to PM2.5 treatment in HUVECs. NONHSAT247851.1 was also identified, which was significantly up-regulated to control the expression of immune response genes. Mechanistically, the results indicated that NONHSAT247851.1 knockdown reduced the expression of IL1ß. In study, we investigated NONHSAT247851.1 as a promoter in regulating immune response genes via binding with raf-1 to regulate the phosphorylation level of p65 protein in HUVECs. The data collected suggests that NONHSAT247851.1 regulates inflammation via interaction with raf-1 to control the inflammatory expression in PM2.5 exposure.


Assuntos
Poluentes Ambientais/toxicidade , Inflamação/induzido quimicamente , Material Particulado/toxicidade , Proteínas Proto-Oncogênicas c-raf/genética , RNA Longo não Codificante/genética , Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/imunologia , Células Endoteliais da Veia Umbilical Humana , Humanos , Inflamação/genética , Interleucina-1beta/genética , Proteínas Proto-Oncogênicas c-raf/metabolismo
10.
Arch Oral Biol ; 113: 104712, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32234582

RESUMO

OBJECTIVE: Chronic stress hormone norepinephrine (NE) has been previously reported to play a role in the development of cancer, but the correlation between NE and oral squamous cell carcinoma (OSCC) progression is not well understood. METHOD: To address this, the expression of adrenergic receptors (ARs) in human OSCC cell lines and clinic OSCC samples was detected, and the role of NEin vivo and in vitro was further investigated. RESULTS: It was found that ß2-AR was the main AR of NE in OSCC. Stimulation of OSCC cells with NE significantly increased the OSCC proliferation and invasion, which was, however, blocked by ß2-AR inhibitor. NE could induce the phosphorylation of extracellular regulated protein kinases (ERK) and cAMP-response element binding protein (CREB). Inhibition of ERK and CREB pathway abrogated NE-induced OSCC invasion and proliferation. NE could enhance cancer stem cells (CSCs)-like phenotype and up-regulate the expression of stemness marker. In tumor-bearing nude mice, it was found that consecutive administration of NE significantly promoted the tumor growth, while daily injection of ß2-AR inhibitor blocked this phenomenon. CONCLUSIONS: Those findings indicated a critical role of the chronic stress hormone NE in OSCC progression. Inhibition of ß2-AR may serve as a potential therapeutic strategy for protecting OSCC patients from chronic stress related deleterious effect.

11.
J Tradit Chin Med ; 40(1): 121-127, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32227773

RESUMO

OBJECTIVE: To investigate the effectiveness of electroacupuncture at Jiaji acupoints (EX-B 2) plus moxibustion and intermediate frequency on postherpetic neuralgia (PHN). METHODS: A total of 140 outpatients who satisfied the inclusion criteria and volunteered for this treatment were randomly divided into treatment (n = 70) and control (n = 70) groups. Both groups received a localized lesion area and electroacupuncture treatment combined with moxibustion and intermediate frequency. The treatment group (TG) increased acupuncture at Jiaji acupoints (EX-B 2) and electroacupuncture. Pain and anxiety were assessed before and after 5, 10, 15, and 20 treatments by using visual pain simulation score (VAS) and Hamilton anxiety scale (HAMA), respectively. Clinical efficacy was also evaluated. RESULTS: The baseline between the two groups did not significantly differ (P > 0.05). The VAS and HAMA scores of the two groups after treatment significantly decreased compared with those of various treatment stages (P > 0.05). The HAMA score (P < 0.01) of TG was lower than that of the control group (CG). The VAS score of TG was lower than that of CG in the 5th and 10th treatments (P < 0.01). In the 15th and 10th scores, CG was also superior to TG (P < 0.05). CONCLUSION: The combined treatment of electroacupuncture at Jiaji acupoints (EX-B 2), moxibustion, and intermediate frequency can relieve the pain and anxiety symptoms of PHN. The efficacy of the combined treatment was superior to traditional acupuncture.

12.
Cancer Chemother Pharmacol ; 85(5): 881-897, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32246190

RESUMO

PURPOSE: High-dose methotrexate (HD-MTX) is widely used in the treatment of non-Hodgkin lymphoma (NHL), but the pharmacokinetic properties of HD-MTX in Chinese adult patients with NHL have not yet been established through an approach that integrates genetic covariates. The purposes of this study were to identify both physiological and pharmacogenomic covariates that can explain the inter- and intraindividual pharmacokinetic variability of MTX in Chinese adult patients with NHL and to explore a new sampling strategy for predicting delayed MTX elimination. METHODS: A total of 852 MTX concentrations from 91 adult patients with NHL were analyzed using the nonlinear mixed-effects modeling method. FPGS, GGH, SLCO1B1, ABCB1 and MTHFR were genotyped using the Sequenom MassARRAY technology platform and were screened as covariates. The ability of different sampling strategies to predict the MTX concentration at 72 h was assessed through maximum a posteriori Bayesian forecasting using a validation dataset (18 patients). RESULTS: A two-compartment model adequately described the data, and the estimated mean MTX clearance (CL) was 6.03 L/h (9%). Creatinine clearance (CrCL) was identified as a covariate for CL, whereas the intercompartmental clearance (Q) was significantly affected by the body surface area (BSA). However, none of the genotypes exerted a significant effect on the pharmacokinetic properties of MTX. The percentage of patients with concentrations below 0.2 µmol/L at 72 h decreased from 65.6 to 42.6% when the CrCL decreased from 90 to 60 ml/min/1.73 m2 with a scheduled dosing of 3 g/m2, and the same trend was observed with dose regimens of 1 g/m2 and 2 g/m2. Bayesian forecasting using the MTX concentrations at 24 and 42 h provided the best predictive performance for estimating the MTX concentration at 72 h after dosing. CONCLUSIONS: The MTX population pharmacokinetic model developed in this study might provide useful information for establishing personalized therapy involving MTX for the treatment of adult patients with NHL.

13.
Org Biomol Chem ; 18(18): 3512-3521, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32334424

RESUMO

Early evaluation of the therapy efficiency can promote the development of anti-tumor drugs and optimization of the treatment method. Caspase-3 is a key biomarker for early apoptosis. Detection of caspase-3 activity is essential for quick assessment of the curative effect. We have reported a PET probe that could image drug-induced tumor apoptosis in vivo. However, high liver uptake limits its application. In order to optimize the pharmacokinetics of the previous probe, we introduced a hydrophilic peptide sequence to minimize liver uptake. The structure of the new probe was confirmed by mass spectrometry and nuclear magnetic resonance. This probe was able to cross the cell membrane freely and could be converted into a dimer through the condensation reaction of 2-cyano-6-aminobenzothiazole (CBT) and cysteine in response to intracellular activated caspase-3 and glutathione (GSH). The hydrophobic dimers further self-assembled into nanoparticles, which could enhance the probe aggregation in apoptotic tumor tissues. In vivo experiments showed that the tumor uptake of the new probe was higher than that of the previous probe, while the liver uptake of the new probe was significantly reduced. The new probe might be promising in imaging apoptotic tumors with suitable pharmacokinetics.

14.
J Steroid Biochem Mol Biol ; 200: 105672, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32311429

RESUMO

Vitamin D deficiency is a global health problem in all age groups, especially in the elderly population. Serum 25(OH)D is the biomarker to assess vitamin D nutrition status. However, the free hormone hypothesis proposes that free vitamin D might be a more reliable marker of vitamin D nutrition status. Thus, the aims of this study were to (1) evaluate the distribution of free 25OHD in elderly individuals, and (2) to assess the association between free 25OHD and total 25(OH)D, 1,25(OH)2D, 24,25(OH)2D, calcium (Ca), alkaline phosphatase (ALP), and phosphorus (P) in elderly population. A total of 312 healthy elderly individuals were enrolled in this study and residual serum samples were collected. Free 25OHD, total 25(OH)D, 24,25(OH)2D, and 1,25(OH)2D were measured using LC-MS/MS. Other biochemical analytes were measured using automatic analyzers. Our results showed that with an increase in the levels of total 25(OH)D, the levels of 25(OH)D3, 1,25(OH)2D, 24,25(OH)D, and free 25OHD increased, whereas the levels of 25(OH)D/24,25(OH)2D decreased. Further, we observed that the level of free 25OHD was significantly positively correlated with the total 25(OH)D (r = 0.226, P < 0.001), 25(OH)D (r = 0.221, P < 0.001), and 24,25(OH)2D (r = 0.231, P < 0.001) but was negatively correlated with 25(OH)D/24,25(OH)2D (r = -0.185, P < 0.01). Moreover, the total 25(OH)D, 25(OH)D3, 24,25(OH)2D, and 25(OH)D/24,25(OH)2D were correlated with 1,25(OH)2D. Furthermore, free 25OHD was positively correlated with creatinine (Cr) (r = 0.227, P <0.001). Our results showed a narrower distribution for free 25OHD than that reported by direct measurement techniques and confirmed the correlation between free 25OHD and total 25(OH)D.

15.
Int J Mol Sci ; 21(8)2020 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-32295035

RESUMO

Autophagy is a highly conserved intracellular degradation pathway that breaks down damaged macromolecules and/or organelles. It is involved in plant development and senescence, as well as in biotic and abiotic stresses. However, the autophagy process and related genes are largely unknown in citrus. In this study, we identified 35 autophagy-related genes (CsATGs-autophagy-related genes (ATGs) of Citrus sinensis, Cs) in a genome-wide manner from sweet orange (Citrus sinensis). Bioinformatic analysis showed that these CsATGs were highly similar to Arabidopsis ATGs in both sequence and phylogeny. All the CsATGs were randomly distributed on nine known (28 genes) and one unknown (7 genes) chromosomes. Ten CsATGs were predicted to be segmental duplications. Expression patterns suggested that most of the CsATG were significantly up- or down-regulated in response to drought; cold; heat; salt; mannitol; and excess manganese, copper, and cadmium stresses. In addition, two ATG18 members, CsATG18a and CsATG18b, were cloned from sweet orange and ectopically expressed in Arabidopsis. The CsATG18a and CsATG18b transgenic plants showed enhanced tolerance to osmotic stress, salt, as well as drought (CsATG18a) or cold (CsATG18b), compared to wild-type plants. These results highlight the essential roles of CsATG genes in abiotic stresses.

16.
BMC Genomics ; 21(1): 233, 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32171259

RESUMO

BACKGROUND: Iron (Fe) deficiency is a common problem in citrus production. As the second largest superfamily of transcription factors (TFs), the basic/helix-loop-helix (bHLH) proteins have been shown to participate in the regulation of Fe homeostasis and a series of other biological and developmental processes in plants. However, this family of members in citrus and their functions in citrus Fe deficiency are still largely unknown. RESULTS: In this study, we identified a total of 128 CgbHLHs from pummelo (Citrus grandis) genome that were classified into 18 subfamilies by phylogenetic comparison with Arabidopsis thaliana bHLH proteins. All of these CgbHLHs were randomly distributed on nine known (125 genes) and one unknown (3 genes) chromosomes, and 12 and 47 of them were identified to be tandem and segmental duplicated genes, respectively. Sequence analysis showed detailed characteristics of their intron-exon structures, bHLH domain and conserved motifs. Gene ontology (GO) analysis suggested that most of CgbHLHs were annotated to the nucleus, DNA-binding transcription factor activity, response to abiotic stimulus, reproduction, post-embryonic development, flower development and photosynthesis. In addition, 27 CgbHLH proteins were predicted to have direct or indirect protein-protein interactions. Based on GO annotation, RNA sequencing data in public database and qRT-PCR results, several of CgbHLHs were identified as the key candidates that respond to iron deficiency. CONCLUSIONS: In total, 128 CgbHLH proteins were identified from pummelo, and their detailed sequence and structure characteristics and putative functions were analyzed. This study provides comprehensive information for further functional elucidation of CgbHLH genes in citrus.

17.
Biochem Biophys Res Commun ; 525(4): 997-1003, 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32178876

RESUMO

C6-ceramide is an exogenous short-chain ceramide which can induce apoptosis of multiple cancer cells. Salivary adenoid cystic carcinoma (SACC) is a common salivary gland cancer, which possesses of high rate of local recurrence and distant metastasis. The mechanism of ceramide-induced SACC-83 and SACC-LM cell apoptosis has not been revealed. In our study, gene expression microarray was used to discover that the unfolded protein response (UPR) pathway, especially PRKR-like endoplasmic reticulum kinase (PERK) pathway, was the major activated pathway after treatment of c6-ceramide. D1ER, an endoplasmic-reticulum-targeted Ca2+ indicator, was used to measure Ca2+ release from endoplasmic reticulum (ER) dynamically. We found that inositol 1,4,5-trisphosphate receptor 3 (IP3R3) was activated, leading to Ca2+ release from ER, soon after c6-ceramide treatment. IP3R3 silencing could block UPR, although it could not prevent SACC-83 and SACC-LM cells from apoptosis. Moreover, we found that C/EBP-homologous protein could upregulate in a UPR-independent way. Mitochondria outer membrane permeabilization might play an important role in inducing SACC cell apoptosis.

18.
BMB Rep ; 53(5): 254-259, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32172731

RESUMO

Increasing evidence suggests the role of miR-449a in the regulation of tumorigenesis and autophagy. Autophagy plays an important role in the malignancy of T-cell lymphoma. However, it is still unknown whether miR-449a is associated with autophagy to regulate the malignancy of T-cell lymp homa. In this study, we for the first time demonstrated that miR-449a enhanced apoptosis of T-cell lymphoma cells by decreasing the degree of autophagy. Further, miR-449a downregulated autophagy-associated 4B (ATG4B) expression, which subsequently reduced the autophagy of T-cell lymphoma cells. Mechanistically, miR-449a decreased ATG4B protein level by binding to its mRNA 3'UTR, thus reducing the mRNA stability. In addition, studies with nude mice showed that miR-449a significantly inhibited lymphoma characteristics in vivo. In conclusion, our results demonstrated that the "miR-449a/ATG4B/autophagy" pathway played a vital role in the malignancy of T-cell lymphoma, suggesting a novel therapeutic target. [BMB Reports 2020; 53(5): 254-259].

19.
BMC Public Health ; 20(1): 297, 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32143667

RESUMO

BACKGROUND: Metabolic syndrome (MetS) is common in China, which has a multi-ethnic population of 1·3 billion. We set out to determine the prevalence of MetS and its components in different ethnic groups. METHODS: This nationwide cross-sectional survey involved 24,796 participants from eight ethnicities in six provinces in China from 2008 to 2011. MetS was defined using the modified National Cholesterol Education Program Adult Treatment Panel III criteria. Results were analysed using SPSS version 22·0 in 2018. Logistic regression was used for deriving odds ratios and 95% confidence intervals of risk factors for the MetS. RESULTS: The prevalence of MetS increased with age from 3·60% to 21·68%. After age standardization, the prevalence of MetS, in descending order, was 35·42% (Korean), 22·82% (Hui), 19·80% (Han), 13·72% (Miao), 12·90% (Tujia), 12·04% (Li), 11·61% (Mongolian), 6·17% (Tibetan). Korean ethnicity was associated with a higher prevalence in five components of MetS, while Tibetan ethnicity was associated with lower prevalence except decreased HDL cholesterol. Logistic regression analyses showed that age, drinking and being non-Tibetan were associated with a higher risk of MetS. CONCLUSIONS: Within one country, albeit a large one, the prevalence of MetS can vary greatly. Chinese of Korean ethnicity had a much higher prevalence than Tibetan ethnicity. Measures to tackle MetS should be tailored to the ethnic groups within a population.


Assuntos
Grupos Étnicos/estatística & dados numéricos , Síndrome Metabólica/etnologia , Adolescente , Adulto , Idoso , Criança , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Adulto Jovem
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