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1.
Oncogene ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31485019

RESUMO

Exosomes are emerging as important elements that participate in intercellular communication and tumor microenvironment modulation, but the exact mechanisms by which tumor exosomes facilitate the generation of the immunosuppressive microenvironment remain unclear. Here we investigated the effects of glioma-derived exosomes (GDEs) on macrophage polarization and glioma progression. We also performed microRNA sequencing analysis of GDEs to identify the microRNA that mediated macrophage polarization. The microRNA-associated intracellular signaling pathway in macrophages was further investigated. Compared with normoxic glioma-derived exosomes (N-GDEs), hypoxic glioma-derived exosomes (H-GDEs) markedly induced M2 macrophage polarization, which subsequently promoted glioma proliferation, migration and invasion in vitro and in vivo. MicroRNA sequencing analysis identified miR-1246 as the most enriched microRNA in H-GDEs. Moreover, miR-1246 was enriched in the CSF of GBM patients and decreased after tumor resection. Further investigation determined that miR-1246 mediated H-GDE-induced M2 macrophage polarization by targeting TERF2IP to activate the STAT3 signaling pathway and inhibit the NF-κB signaling pathway. Our study elucidated a mechanism by which hypoxia and glioma influence M2 macrophage polarization via exosomes, which could facilitate the formation of the immunosuppressive microenvironment. Moreover, our results suggested that miR-1246 in the CSF of GBM patients may be a novel biomarker for GBM diagnosis and that treatment targeting microRNA-1246 may contribute to antitumor immunotherapy.

2.
Appl Opt ; 58(19): 5257-5261, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31503622

RESUMO

In this paper, we propose a new pumping scheme-a dual-frequency laser-pumped cascade structure of Er3+-doped optical fiber based on the theory of coherent population oscillation. While realizing slow light transmission, the loss of output signal light power is reduced. Using numerical simulation, we compare the effects of a single fiber without pumping, a 980 nm single pumping single fiber, and a dual-frequency laser-pumped cascade structure of Er3+-doped optical fiber on signal light intensity loss coefficient τ and maximum time delay with the increase of input signal light power. The results show that the signal light intensity loss coefficient τ of the three structures decreases gradually with the increase of input signal light power. However, the transmission of lower signal light intensity loss coefficient τ=0.0823 and a larger time delay of 0.15 ms can be obtained under the dual-frequency laser-pumped cascade structure of Er3+-doped optical fiber. On the basis of the dual-frequency laser-pumped cascade structure of Er3+-doped optical fiber, the signal light intensity loss coefficient τ decreases gradually with the increase of pump ratio M (M=P1480∶P980) and the decrease of length ratio N (N=L2∶L1; L1 and L2 represent the length of the first and second cascaded optical fibers, respectively). Moreover, we compare the effects of ion density of the Er3+-doped optical fiber on the time delay of three structures. The dual-frequency laser-pumped cascade structure of Er3+-doped optical fiber can provide a larger time delay and smaller signal light intensity loss coefficient τ at low ion density of the Er3+-doped optical fiber.

3.
Asian J Surg ; 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-31402083

RESUMO

OBJECTIVE: This study is aim to summarize the experience of robotic thyroidectomy via bilateral axillo-breast approach of our center and also to find out the learning curve of this technique. METHODS: In total 220 initial patients who have undergone robotic thyroidectomy via bilateral axillo-breast approach from May 2015 to September 2017 were involved in this study. The data of operation time, clinical characteristics, surgical outcomes and oncological outcomes were collected. The moving average method is use to explore the learning curve. RESULTS: All patients had undergone robotic thyroidectomy successfully without conversion to other surgical approaches. The mean age of the enrolled subjects was 34.4 ± 7.8 years old, while the sex ratio (male/female) was 38/182. There were 50 benign tumor cases and 170 malignant tumor cases. The mean total operation time was 105.3 ± 37.6 min. Lymph node metastasis was observed in 61 (35.9%) patients. The mean retrieved lymph node count was 5.1 ± 3.8 while the mean metastatic lymph node count was 0.7 ± 1.5. The operation time decreased significantly after about 30-35 cases and formed the plateau. After 80 cases, the operation time significantly decreased again. CONCLUSION: For skilled endocrine surgeons, robotic thyroidectomy has proved to be safe and feasible, which could be applied extensively in patients strictly selected in high-volume centers, with a relatively short learning curve of about 30-35 cases. While the surgeons getting more experienced, this technique would be more efficient.

4.
Water Res ; 164: 114957, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31421513

RESUMO

Iron chelators are often used to improve the performance of Fe(II) activated peroxides (e.g., peroxydisulfate (PDS) and hydrogen peroxide (H2O2)) for oxidative water treatment over a wide pH range due to the enhanced solubility of iron in the presence of chelators at high pH. In this study, we compared the effect of various chelators on the production and nature of the reactive intermediate formed in Fe(II)/PDS and Fe(II)/H2O2 systems by using methyl phenyl sulfoxide (PMSO) as a probe, which could distinguish ferryl ion (Fe(IV)) from free radicals (•OH and SO4•-) due to their marked difference in product formation. Six representative chelators (oxalate acid (OA), citric acid (CA), nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), pyrophosphate (PPP), and tetrapolyphosphate (TPP)) which covered the commonly used polycarboxylates, aminocarboxylates, and polyphosphates ligands were selected. In chelator assisted Fe(II)/PDS systems, the highest PMSO transformation efficiency at pH 3-9 was obtained in cases with polycarboxylates, due to their higher reactivity to PDS activation, lower steric hindrance, and stronger ability in promoting Fe(II)/Fe(III) cycle. Comparatively, in chelator assisted Fe(II)/H2O2 systems, TPP addition achieved the best performance in PMSO transformation at pH > 5. Moreover, the yield of Fe(IV) indicative product (methyl phenyl sulfone, PMSO2) decreased with increasing chelator/Fe(II) molar ratio, but was independent on pH in cases of PDS, indicating that chelator altered reactive intermediate nature from Fe(IV) to SO4•- and Fe(IV) yield was not sensitive to pH. In cases of H2O2, chelator decreased PMSO2 production while promoting PMSO loss at near-neutral pH, suggesting that Fe(II)-chelator complexes also tended to catalyze H2O2 to generate •OH rather than Fe(IV).

5.
Artigo em Inglês | MEDLINE | ID: mdl-31434633

RESUMO

Dr. Cyprian's study show no significant different in electrochemical skin conductance between Parkinson's disease patients and controls, which is inconsistent with our recently published data. We propose that this difference may caused by the bias of patient selection and levodopa application.

6.
J Sci Food Agric ; 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31435952

RESUMO

BACKGROUND: Oyster polypeptides have various biofunctions, such as the anti-cancer and the anti-oxidative stress, but whether it has the protective effects to primary ovarian failure (POF) remains poorly understand. To address this issue, daily gavage of oyster polypeptides was performed to investigate their protective effect for POF, basing on D-galactose-reduced POF model in C57BL/6 female mice. RESULTS: Oyster polypeptides restored the irregular estrous cycles and the abnormal serum FSH, LH and P levels as well as the decreased mRNA expression level of Amh that were induced by D-gal. The follicle development of POF mice was improved by increasing the primordial follicle ratio and decreasing the atretic follicle number after oral administration of oyster polypeptides. Moreover, in the oyster polypeptides treated mice, the total SOD (T-SOD) activity was significantly increased, while the MDA levels were significantly decreased. The mRNA expression levels of stress-related genes (SOD2, SIRT1 and FOXO3a) were remarkably up-regulated after D-gal induction, but the up-regulation was weakened or disappeared by the gavage of oyster polypeptides. In addition, oyster polypeptides treatment also reduced the apoptosis of the ovarian granulosa cells and down-regulated the mRNA expression levels of apoptosis-related genes (p53 and Bad but not Bcl-2). CONCLUSION: This study reveals that oyster polypeptides may protect ovary against D-gal-induced POF by their anti-oxidative stress activity to rescue D-gal-induced ovarian oxidative damage and therefore to prevent ovarian cells apoptosis, thereby tipping the abnormality trigged by POF to get close to the normal levels. This article is protected by copyright. All rights reserved.

7.
JAMA Netw Open ; 2(8): e199826, 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31433485

RESUMO

Importance: Preclinical studies suggest that amylin has a U-shaped dose-response association with risk of Alzheimer disease (AD). The association of plasma amylin with AD in humans is unknown. Objectives: To measure amylin concentration in plasma by using enzyme-linked immunosorbent assay and to study the association between plasma amylin, incidence of AD, and brain structure in humans. Design, Setting, and Participants: This cohort study used data from the Framingham Heart Study offspring cohort from 1998 to 2015. Using a Monte Carlo approach, participants were divided into 3 plasma amylin concentration groups: (1) low (<75 pmol/L), (2) high (75-2800 pmol/L), and (3) extremely high (≥2800 pmol/L). Data analyses were conducted October 5, 2017, to December 18, 2018. Exposures: Baseline plasma amylin concentrations at examination 7. Main Outcomes and Measures: Incidence of dementia or AD and brain volumetric measures from structural magnetic resonance imaging data. Results: From the Framingham Heart Study offspring cohort, 3061 participants (mean [SD] age at baseline, 61.0 [9.5] years; 1653 [54.0%] women) who had plasma amylin measurements, dementia incidence, and brain volume measurements on record were included in this study. The distribution of plasma amylin concentrations was highly skewed (median [interquartile range], 7.5 [4.6-18.9] pmol/L; mean [SD], 302.3 [1941.0] pmol/L; range, 0.03-44 623.7 pmol/L). Compared with the low plasma amylin concentration group, the high plasma amylin concentration group had a lower rate of AD incidence (2.3% vs 5.6%; P = .04), but the extremely high plasma amylin concentration group had a higher rate of AD incidence (14.3%; P < .001). After adjusting for age, sex, education, body mass index, diabetes, cardiovascular disease, high-density lipoprotein level, and APOE4, high plasma amylin was not associated with decreased AD risk (hazard ratio, 0.42 [95% CI, 0.16-1.14]; P = .09) but was positively associated with volume of gray matter in the temporal lobe (ß = 0.17 [SE, 0.05]; P < .001). In contrast, extremely high plasma amylin concentration was associated with a higher AD risk (hazard ratio, 2.51 [95% CI, 1.38-4.57]; P = .003) but not associated with temporal lobe volume (ß = 0.02 [SE, 0.07]; P = .82). Conclusions and Relevance: This study found that plasma amylin concentration was associated with AD incidence and brain structure with a U-shaped pattern. These findings are consistent with preclinical findings that suggest amylin is a neuropeptide that is physiological; however, at extremely high concentrations, it may lead to amylin aggregation and therefore may be a risk factor for AD.

8.
J Neurol Sci ; 405: 116430, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31465985

RESUMO

Few studies have compared radiological features obtained on magnetic resonance imaging (MRI) between myelin oligodendrocyte glycoprotein antibody (MOG-ab)- and aquaporin 4 antibody (AQP4-ab)-positive patients. In this study, 77 MOG-ab and 92 AQP4-ab patients were enrolled. The results demonstrated that the brain MRI-based incidence of subcortical white matter lesions was higher in MOG-ab patients (p < .000) than in AQP4-ab patients and that the former therefore had a higher incidence of periventricular lesions (p = .003). The posterior limb of the internal capsule was more prone to lesions in MOG-ab patients (p = .019). Large lesions and U- or S-shaped lesions were also more frequent in MOG-ab (p < .000 and p = .013, respectively). Half of the MOG-ab patients had spinal cord involvement, and 36.5% presented with longitudinally extensive transverse myelitis (LETM). However, among the MOG-ab and AQP4-ab patients with spinal attack, there was no significant difference in the proportion with LETM (p = .057). In conclusion, a higher proportion of MOG-ab patients than AQP4-ab patients had brain lesions in white matter. Among MOG-ab patients who had an attack in the spinal cord, 65.5% also had LETM during the disease course. Conus medullaris lesions were rare in Chinese MOG-ab patients.

9.
Water Res ; 165: 114969, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31434015

RESUMO

In this paper, the kinetics of bisphenol S (BPS) degradation in the presence of peroxydisulfate (PDS) or dissolved oxygen (DO) in ultrasound (US) system were investigated. For PDS (US/PDS), increased PDS concentration result in faster BPS degradation, but the enhancement was not remarkable with multiplying PDS dosages. Therefore, heterogeneous PDS activation model based on a Langmuir-type adsorption mechanism was proposed to explain the trait of BPS abatement. The equilibrium constant of PDS (KPDS) was calculated to be 2.91 × 10-4/µM, which was much lower than that of BPS, suggesting that PDS was hard to adsorb on the gas-liquid interface of the cavitation bubble following by activation. Besides, the formation of •OH and SO4•- in US/PDS system was reinvestigated. The result showed that SO4•- rather than •OH was the predominant radical, which was quite different from previous study. Dissolved oxygen largely improve the degradation of BPS in US system and •OH rather than O2•- was proved to be the main reactive oxygen species (ROS). The improvement of •OH generation possibly caused by the reaction of DO with •H so that it cannot recombine with •OH. The transformation of the BPS in US system mainly included BPS radical polymerization, hydroxylation and hydrolysis. Frustratingly, the acute toxicity assay of Vibrio fischeri suggests that the degradation products of BPS are more toxic. These results will improve the understanding on the activation mechanisms of PDS and the role of dissolved oxygen play in US. Further investigations may need to explore other treatment ways of BPS and evaluate the acute toxicity of degradation products.

10.
J Alzheimers Dis ; 70(4): 1025-1040, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31306122

RESUMO

 Recent studies demonstrate that peripheral amylin treatment reduces pathology in mouse models of Alzheimer's disease (AD). However, soluble and aggregated amylin are distinct species; while amylin is a physiological neuropeptide, amylin aggregation is a pathological factor for diabetes. We thus hypothesized that because of their similarity in secondary structures, amylin antagonizes amyloid-ß peptide (Aß)-induced AD pathology in neurons with a dose-dependent pattern. To test the hypothesis, we conducted both in vitro and in vivo experiments with different doses of amylin and with its analog, pramlintide. Here we report that a high concentration of either Aß or amylin alone induced tau phosphorylation (pTau) in primary neurons. Interestingly, with a low concentration, amylin had direct effects to reverse the Aß-induced pTau, as well as damaged neuronal synapses and neurite disorganization. However, when the concentration was high (10.24 µM), amylin lost the effects against the Aß-induced cellular AD pathology and, together with Aß, worsened tauopathy in neurons. In the 5XFAD AD mouse model, daily peripheral amylin treatment with a low dose (200 µg/kg) more effectively reduced amyloid burden, and increased synapse, but with a high dose (800 µg/kg), it more effectively reduced tauopathy. Correspondingly, amylin treatment improved learning and memory in these mice. It demonstrates that amylin has a dose-dependent U-shape effect against AD pathogenesis. Within a physiological range, amylin is a neuroprotective hormone against AD in neurons; but when both Aß and amylin concentrations are elevated, imbalance of Aß and amylin may contribute to brain AD pathogenesis.

11.
Int J Biol Sci ; 15(7): 1396-1403, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31337970

RESUMO

Dendritic cells (DCs) are the most potent specialized antigen-presenting cells as now known, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. Immunologically, the motilities and T cell activation capabilities of DCs are closely related to the resulting immune responses. However, due to the complexity of the immune system, the dynamic changes in the number of cells during the peripheral tissue (e.g. skin and mucosa) immune response induced by DCs are still poorly understood. Therefore, this study simulated dynamic number changes of DCs and T cells in this process by constructing several ordinary differential equations and setting the initial conditions of the functions and parameters. The results showed that these equations could simulate dynamic numerical changes of DCs and T cells in peripheral tissue and lymph node, which was in accordance with the physiological conditions such as the duration of immune response, the proliferation rates and the motilities of DCs and T cells. This model provided a theoretical reference for studying the immunologic functions of DCs and practical guidance for the clinical DCs-based therapy against immune-related diseases.

12.
BMC Infect Dis ; 19(1): 645, 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31324230

RESUMO

BACKGROUND: Long-term outcome of DAAs therapy in kidney transplant recipients was unknown. Thus, we aimed to evaluate it in a Chinese cohort of HCV-infected kidney transplant recipients. METHODS: Single-center and retrospective study of HCV-infected kidney transplant recipients initiating an DAAs regimen between January 2015 and December 2017 was conducted. Totally 26 KTX recipients were divided into three groups, including KTX-HD Group, DAA-KTX Group and KTX-DAA Group. On-treatment response was defined as target not detected within 12 weeks. SVR 48, 96 were defined as HCV-RNA negativity 48, 96 weeks after treatment cessation, respectively. RESULTS: HCV genotype was predominantly 1b (80.8%), followed by 2a. All (100%) patients achieved on-treatment response. Time to first TnD was 1.9 ± 0.6 weeks, with no significant difference among the three groups. All patients achieved SVR, with an SVR rate of 100.0% (26/26) among the patients who were followed up over 48 weeks after treatment cessation, and the same SVR rate (24/24) among the patients who were followed up over 96 weeks. Trough levels of Tac remained stable under DAAs therapy, without any dose adjustment. Two patients with abnormal GFR before treatment experienced serum creatinine elevation. Other adverse events included nausea, diarrhea, acid regurgitation, bilirubin elevation and edema of lower limbs. All patients recovered after treatment cessation without reductions in dose, or withdrawal of DAAs or immunosuppressive agents. CONCLUSIONS: HCV genotype 1b and 2a are the only genotypes and 1b is predominant in our center. Antiviral treatment with DAAs in HCV-infected kidney transplant recipients is persistently effective and well tolerated during long-term follow-up. A regular monitoring of renal function in patients who receive DAAs regimens with preexisting impaired renal function is strongly recommended. Furthermore, the trough CNIs levels were recommended to be frequently monitored.

13.
Int J Clin Oncol ; 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31309383

RESUMO

BACKGROUND: PLEKHG5, a Rho-specific guanine-nucleotide exchange factor, is involved in tumor cell migration, invasion and angiogenic potential. In this study, the expression pattern, prognostic value and function of PLEKHG5 in gliomas were investigated. METHODS: Immunohistochemistry was used to determine the expression pattern of PLEKHG5 in 61 glioma patients after curative resection. Statistical analysis was performed to evaluate the diagnostic and prognostic significance of PLEKHG5. Gene ontology (GO) analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis and Gene set enrichment analysis (GSEA) were used to predict potential functions of PLEKHG5. Migration assay and western blot analysis determined PLEKHG5 function in glioma migration and invasion. RESULTS: Increased PLEKHG5 expression levels were associated with higher glioma grades (P < 0.05). In addition, glioblastomas multiforme have higher ratio and stronger intensity of PLEKHG5 expression compared with low-grade gliomas. High expression level of PLEKHG5 indicated poorer prognosis and shorter survival time in all glioma patients (P < 0.001). GO analysis, KEGG pathway analysis and GSEA analysis suggested that PLEKHG5 was involved in glioma migration, invasion and epithelial-mesenchymal transition. Migration assay and western blot analysis revealed PLEKHG5 promoted glioma migration and invasion. CONCLUSION: Our results demonstrated PLEKHG5 could be used as a novel prognostic biomarker and anti-tumor target for glioma patients.

14.
Biosci Rep ; 39(7)2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31273056

RESUMO

Resistance to doxorubicin (DOX) is the most common clinical problem in breast cancer therapy, and the underlying molecular mechanism remains to be investigated. MicroRNAs (miRNAs) exhibit important regulatory functions in various malignant tumors including breast cancer. The aim of the present study was to find the relationship between miR-222 and DOX resistance. We found that miR-222 was highly expressed in patients' serum and DOX-resistant cell line MCF-7-R and that miR-222 could promote proliferation and migration of breast cancer cells. Our results also showed that inhibition of miR-222 in MCF-7-R significantly increased Bcl-2 interacting mediator (Bim) expression both in mRNA and protein levels by using quantitative real-time PCR (qRT-PCR) and Western blot. MTT and flow cytometry suggested that lower expressed miR-222 enhanced apoptosis and decreased IC50 of MCF-7-R cells. Conversely, in MCF-7 cells transfected with miR-222 mimics, up-regulation of miR-222 was associated with decreased Bim level accompanied by less apoptosis and higher IC50 Moreover, miR-222 inhibitors reversed DOX resistance via miR-222-Bim-caspase pathway. Collectively, these data first elucidated that miR-222 could function as an oncogene and was able to reduce the sensitivity of breast cancer cells to DOX through miR-222-Bim-caspase pathway, which provided a potential target to increase DOX sensitivity in clinical breast cancer treatment.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31329559

RESUMO

Inverse Synthetic Aperture Radar (ISAR) imaging technique of moving target with sparse sampling data has attracted wide attention, due to its ability to reduce the data collection burden. However, traditional low-rank or 2-D Compressive Sensing (CS) based ISAR imaging methods can handle the random sampling or the separable sampling data only. When the specific data collection condition cannot be satisfied, low-rank or 2-D CS based methods cannot provide satisfactory imaging results any more. To remedy this problem, in this paper, we proposed a joint low-rank and sparsity priors constrained model for ISAR imaging with various sparse data patterns. This model is inspired by the facts that the received radar data has a lowrank property and the ISAR image is sparse on the specific dictionary. Two reconstruction algorithms to solve the double priors constrained optimization problem are developed under the alternative direction method of multipliers (ADMM) framework with the help of augmented Lagrange multipliers (ALM). Results on simulation data and real data show that the proposed methods are quite effective in recovering missing samples and focused image, and perform better than the matrix completion based method and the sparse representation based method when dealing with the various kinds of sparse sampling data.

16.
Hepatology ; 2019 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-31297851

RESUMO

We appreciate you and your colleagues' positive comments on our recent publication (1) . In this study, we found that overexpression of both FAK and constitutively active forms of ß-catenin (CAT), but neither FAK nor CAT alone, in mouse livers was sufficient to lead to tumorigenesis. Further, we found that overexpression of FAK dramatically enhances binding of ß-catenin to the promoter of androgen receptor (AR), which leads to increased expression of AR in mouse livers. This article is protected by copyright. All rights reserved.

17.
BMC Neurol ; 19(1): 156, 2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31291907

RESUMO

BACKGROUND: Loss-of-function mutations in the CLCN2 gene were recently discovered to be a cause of a type of leukodystrophy named CLCN2-related leukoencephalopathy (CC2L), which is characterized by intramyelinic edema. Herein, we report a novel mutation in CLCN2 in an individual with leukodystrophy. CASE PRESENTATION: A 38-year-old woman presented with mild hand tremor, scanning speech, nystagmus, cerebellar ataxia in the upper limbs, memory decline, tinnitus, and dizziness. An ophthalmologic examination indicated macular atrophy, pigment epithelium atrophy and choroidal capillary atrophy. Brain magnetic resonance imaging (MRI) showed the diffuse white matter involvement of specific white matter tracts. Decreased diffusion anisotropy was detected in various brain regions of the patient. Diffusion tensor tractography (DTT) showed obviously thinner tracts of interest than in the controls, with a decreased fiber number (FN), increased radial diffusivity (RD) and unchanged axial diffusivity (AD). A novel homozygous c.2257C > T (p.Arg753Ter) mutation in exon 20 of the CLCN2 gene was identified. CONCLUSION: CC2L is a rare condition characterized by diffuse edema involving specific fiber tracts that pass through the brainstem. The distinct MRI patterns could be a strong indication for CLCN2 gene analysis. The findings of our study may facilitate the understanding of the pathophysiology and clinical symptoms of this disease.

18.
J Occup Health ; 61(5): 387-397, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31183937

RESUMO

OBJECTIVE: Data on noise-induced hearing loss (NIHL) in the automotive industry are rare. This pilot study aimed to investigate the prevalence and determinants of NIHL among workers in the automotive industry in China. METHODS: A cross-sectional survey was conducted with 6557 participants from the automotive industry. The questionnaire survey was administered, and individual noise exposure level (LAeq.8h ) and hearing loss level were measured. RESULTS: Of participants, 96.43% were male; the median age was 27.0 years and 28.82% had NIHL defined as adjusted high-frequency noise-induced hearing loss (AHFNIHL). Concerning individual noise levels (LAeq.8h ), 62.53% exceeded 85 dB(A), which were mainly concentrated in various jobs, including metal cutting, surface treatment, stamping, welding, grinding, assembly, plastic molding, and forging. Each typical noise source generated its own unique temporal waveform shape with the type of non-Gaussian noise. Of workers, 53.15% regularly used hearing protector devices (HPD), and the proportion of regular HPD use increased with LAeq.8h . The trend test showed that the prevalence of AHFNIHL in male workers significantly increased with an increase in LAeq.8h at <94 dB(A) and cumulative noise exposure (CNE) in each age group (P < 0.05 or P < 0.01). A logistic regression analysis showed that CNE and HPD usage frequency were important factors contributing to AHFNIHL. CONCLUSIONS: CNE and HPD usage frequency were the determinants for NIHL. Much more human surveys are needed to understand the prevalence and determinants of NIHL in the automotive industry in China.

19.
BMC Bioinformatics ; 20(Suppl 8): 289, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182017

RESUMO

BACKGROUND: Gene selection is one of the critical steps in the course of the classification of microarray data. Since particle swarm optimization has no complicated evolutionary operators and fewer parameters need to be adjusted, it has been used increasingly as an effective technique for gene selection. Since particle swarm optimization is apt to converge to local minima which lead to premature convergence, some particle swarm optimization based gene selection methods may select non-optimal genes with high probability. To select predictive genes with low redundancy as well as not filtering out key genes is still a challenge. RESULTS: To obtain predictive genes with lower redundancy as well as overcome the deficiencies of traditional particle swarm optimization based gene selection methods, a hybrid gene selection method based on gene scoring strategy and improved particle swarm optimization is proposed in this paper. To select the genes highly related to out samples' classes, a gene scoring strategy based on randomization and extreme learning machine is proposed to filter much irrelevant genes. With the third-level gene pool established by multiple filter strategy, an improved particle swarm optimization is proposed to perform gene selection. In the improved particle swarm optimization, to decrease the likelihood of the premature of the swarm the Metropolis criterion of simulated annealing algorithm is introduced to update the particles, and the half of the swarm are reinitialized when the swarm is trapped into local minima. CONCLUSIONS: Combining the gene scoring strategy with the improved particle swarm optimization, the new method could select functional gene subsets which are significantly sensitive to the samples' classes. With the few discriminative genes selected by the proposed method, extreme learning machine and support vector machine classifiers achieve much high prediction accuracy on several public microarray data, which in turn verifies the efficiency and effectiveness of the proposed gene selection method.


Assuntos
Algoritmos , Genes , Bases de Dados Genéticas , Humanos , Aprendizado de Máquina , Neoplasias/genética
20.
Cancer Sci ; 110(8): 2357-2367, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31169331

RESUMO

Dendritic cells (DCs) are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses against cancer. Tumor cells can escape from immune attack by secreting suppressive cytokines that solely or cooperatively impair the immune function of DCs. However, the underlying mechanisms are not fully defined. Vascular endothelial growth factor (VEGF) has been identified as a major cytokine in the tumor microenvironment. To elucidate the effects of VEGF on the motility and immune function of mature DCs (mDCs), the cells were treated with 50 ng/mL VEGF and investigated by proteomics and molecular biological technologies. The results showed that VEGF can impair the migration capacity and immune function of mDCs through the RhoA-cofilin1 pathway mediated by the VEGF receptor 2, suggesting impaired motility of mDCs by VEGF is one of the aspects of immune escape mechanisms of tumors. It is clinically important to understand the biological behavior of DCs and the immune escape mechanisms of tumor as well as how to improve the efficiency of antitumor therapy based on DCs.


Assuntos
Fatores de Despolimerização de Actina/metabolismo , Movimento Celular/imunologia , Células Dendríticas/metabolismo , Transdução de Sinais/imunologia , Fator A de Crescimento do Endotélio Vascular/imunologia , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Proteína rhoA de Ligação ao GTP/metabolismo , Fatores de Despolimerização de Actina/imunologia , Células Cultivadas , Citocinas/imunologia , Células Dendríticas/imunologia , Células Endoteliais da Veia Umbilical Humana , Humanos , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/imunologia , Proteína rhoA de Ligação ao GTP/imunologia
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