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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117675, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31670047

RESUMO

By modifying the 10-butyl-2-methoxy-10H-phenothiazine-3-carbaldehyde with malonontrile group, a new fluorescent sensor PBM for selective detection of hydrazine in ratiometric mode has been developed. Probe PBM owned the advantages of quick response (10 min), remarkable Stokes shift (168 nm for PBM, 161 nm for PBM-NH2), excellent selectivity, high sensitivity (detection limit of 63.2 nM was obtained from in vitro experiment), profound ratiometric change (82-fold) and low cytotoxicity in response to hydrazine. Additionally, it could be utilized to monitor hydrazine in gas state with various concentrations through vivid color changes and imaged hydrazine in living MCF-7 cells with excellent performance.

2.
Sci Rep ; 5: 12010, 2015 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-26131602

RESUMO

The lipocalin proteins (lipocalins) are a large family of small proteins characterized by low sequence similarity and highly conserved crystal structures. Lipocalins have been found to play important roles in many human diseases. For this reason, a systemic analysis of the molecular properties of human lipocalins is essential. In this study, human lipocalins were found to contain four structurally conserved regions (SCRs) and could be divided into two subgroups. A human lipocalin protein-protein interaction network (PPIN) was constructed and integrated with their expression data in esophageal carcinoma. Many lipocalins showed obvious co-expression patterns in esophageal carcinoma. Their subcellular distributions also suggested these lipocalins may transfer signals from the extracellular space to the nucleus using the pathway-like paths. These analyses also expanded our knowledge about this human ancient protein family in the background of esophageal carcinoma.


Assuntos
Neoplasias Esofágicas/metabolismo , Lipocalinas/metabolismo , Sequência de Aminoácidos , Expressão Gênica , Humanos , Lipocalinas/química , Lipocalinas/genética , Anotação de Sequência Molecular , Dados de Sequência Molecular , Mapas de Interação de Proteínas , Transporte Proteico , Proteína do Retinoblastoma/metabolismo , Homologia de Sequência de Aminoácidos , Transdução de Sinais
3.
Asian Pac J Cancer Prev ; 16(13): 5445-51, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26225692

RESUMO

Fascin-1 (FSCN1) is an actin-bundling protein that induces cell membrane protrusions, increases cell motility, and is overexpressed in various human epithelial cancers, including esophageal squamous cell carcinoma (ESCC). We analyzed various protein-protein interactions (PPI) of differentially-expressed genes (DEGs), in fascin knockdown ESCC cells, to explore the role of fascin overexpression. The node-degree distributions indicated these PPI sub-networks to be characterized as scale-free. Subcellular localization analysis revealed DEGs to interact with other proteins directly or indirectly, distributed in multiple layers of extracellular membrane-cytoskeleton/ cytoplasm-nucleus. The functional annotation map revealed hundreds of significant gene ontology (GO) terms, especially those associated with cytoskeleton organization of FSCN1. The Random Walk with Restart algorithm was applied to identify the prioritizations of these DEGs when considering their relationship with FSCN1. These analyses based on PPI network have greatly expanded our comprehension of the mRNA expression profile following fascin knockdown to future examine the roles and mechanisms of fascin action.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma de Células Escamosas/genética , Proteínas de Transporte/antagonistas & inibidores , Neoplasias Esofágicas/genética , Regulação Neoplásica da Expressão Gênica , Redes Reguladoras de Genes , Proteínas dos Microfilamentos/antagonistas & inibidores , RNA Interferente Pequeno/genética , Biomarcadores Tumorais/metabolismo , Carcinoma de Células Escamosas/metabolismo , Proteínas de Transporte/genética , Neoplasias Esofágicas/metabolismo , Perfilação da Expressão Gênica , Humanos , Proteínas dos Microfilamentos/genética , Mapas de Interação de Proteínas , Células Tumorais Cultivadas
4.
Asian Pac J Cancer Prev ; 15(16): 6899-904, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25169543

RESUMO

NGAL (neutrophil gelatinase-associated lipocalin) is a novel cancer-related protein involves multiple functions in many cancers and other diseases. We previously overexpressed NGAL to analyze its role in esophageal squamous cell carcinoma (ESCC). In this study, a protein-protein interaction (PPI) was constructed and the shortest paths from NGAL to transcription factors in the network were analyzed. We found 28 shortest paths from NGAL to RELA, most of them obeying the principle of extracellular to cytoplasm, then nucleus. These shortest paths were also prioritized according to their normalized intensity from the microarray by the order of interaction cascades. A systems approach was developed in this study by linking differentially expressed genes with publicly available PPI data, Gene Ontology and subcellular localizaton for the integrated analyses. These shortest paths from NGAL to DEG transcription factors or other transcription factors in the PPI network provide important clues for future experimental identification of new pathways.


Assuntos
Proteínas da Fase Aguda/genética , Carcinoma de Células Escamosas/patologia , Neoplasias Esofágicas/patologia , Lipocalinas/genética , Mapas de Interação de Proteínas/genética , Proteínas Proto-Oncogênicas/genética , Fatores de Transcrição/genética , Proteínas da Fase Aguda/biossíntese , Proteínas da Fase Aguda/metabolismo , Linhagem Celular Tumoral , Carcinoma de Células Escamosas do Esôfago , Humanos , Lipocalina-2 , Lipocalinas/biossíntese , Lipocalinas/metabolismo , Proteínas Proto-Oncogênicas/biossíntese , Proteínas Proto-Oncogênicas/metabolismo , RNA Mensageiro/biossíntese
5.
Bioorg Med Chem ; 21(2): 448-55, 2013 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-23245802

RESUMO

A series of novel thiazolyl-pyrazoline derivatives containing benzodioxole (C1-C20) have been designed and synthesized. Among of the synthesized compounds, 2-(5-(benzo[d][1,3]dioxol-5-yl)-3-(4-bromophenyl)-4,5-dihydro-1H-pyrazol-1-yl)-4-(4-bromophenyl)thiazole (C6) displayed the most potent inhibitory activity for HER-2 (IC(50) = 0.18 µM for HER-2). Antiproliferative assay results indicated that compound C6 owned high antiproliferative activity against MCF-7 and B16-F10 in vitro, with IC(50) value of 0.09 and 0.12 µM, respectively, being comparable with the positive control Erlotinib. Docking simulation was further performed to determine the probable binding model. Based on the preliminary results, compound C6 with potent inhibitory activity in tumor growth would be a potential anticancer agent.


Assuntos
Antineoplásicos/síntese química , Benzodioxóis/química , Pirazóis/química , Tiazóis/química , Antineoplásicos/química , Antineoplásicos/toxicidade , Sítios de Ligação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Humanos , Células MCF-7 , Simulação de Acoplamento Molecular , Inibidores de Proteínas Quinases/síntese química , Inibidores de Proteínas Quinases/química , Inibidores de Proteínas Quinases/toxicidade , Estrutura Terciária de Proteína , Pirazóis/síntese química , Pirazóis/toxicidade , Receptor ErbB-2/antagonistas & inibidores , Receptor ErbB-2/metabolismo , Relação Estrutura-Atividade
6.
Acta Crystallogr Sect E Struct Rep Online ; 65(Pt 5): o975, 2009 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-21584015

RESUMO

The title Schiff base compound, C(14)H(11)ClN(2)O(3), was prepared by the reaction of 5-chloro-salicylaldehyde and 4-hydroxy-benzohydrazide. The mol-ecule exists in a trans configuration with respect to the methyl-idene group. The dihedral angle between the two benzene rings is 40.1 (2)°. An intra-molecular O-H⋯N hydrogen bond helps to stabilize the mol-ecular conformation. In the crystal structure, mol-ecules are linked into a three-dimensional network by inter-molecular N-H⋯O and O-H⋯O hydrogen bonds.

7.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 9): o1829-30, 2008 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-21201804

RESUMO

The title Schiff base compound, C(19)H(16)N(2)O(3), prepared by the reaction of 2-meth-oxy-1-naphthyl-aldehyde and 2-hydroxy-benzohydrazide, crystallizes with two independent mol-ecules in the asymmetric unit. Each mol-ecule exists in a trans configuration with respect to the methyl-idene group. The naphthyl ring system make dihedral angles of 65.0 (2)° and 55.8 (2)° with the planes of the benzene rings. Intra-molecular N-H⋯O and O-H⋯O hydrogen bonds help to stabilize the mol-ecular conformations. In the crystal structure, mol-ecules are linked into one-dimensional chains parallel to the c axis by inter-molecular O-H⋯N and O-H⋯O hydrogen bonds.

8.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 9): o1831, 2008 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-21201805

RESUMO

The mol-ecule of the title Schiff base compound, C(20)H(18)N(2)O(3), prepared by the reaction of 2-meth-oxy-1-naphthyl-aldehyde and 4-methoxy-benzohydrazide, exists in a trans configuration with respect to the imine group. The naphthyl ring system makes a dihedral angle of 71.4 (2)° with the mean plane of the benzene ring. In the crystal structure, mol-ecules are linked into one-dimensional chains parallel to the c axis by inter-molecular N-H⋯O hydrogen bonds.

9.
World J Gastroenterol ; 11(28): 4305-10, 2005 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-16038025

RESUMO

AIM: To investigate the relationship between the superoxide dismutase (SOD), malondialdehyde (MDA) metabolic changes and the gastric carcinogenesis. METHODS: The SOD activity and MDA content were measured in the gastric tissues from the focus center, peripheral and far-end areas of gastric carcinoma (n = 52) and gastric ulcer (n = 10). All the tissues were subjected to routine histological examinations and classifications. RESULTS: The SOD activity was greatly reduced but the MDA content was markedly increased in the center areas of the non-mucous gastric carcinoma (non-MGC); and the poorly differentiated gastric carcinoma varied. The SOD activity was gradually decreased and the MDA content was gradually increased in the tissues from the focus far-end, peripheral to center areas of non-MGC. Both of the SOD activity and the MDA content were significantly declined and were respectively at same low level in the tissues from the focus center, peripheral, and far-end area with the mucous gastric carcinoma (MGC). In contrast to the gastric ulcer and grade I or II of non-MGC, the same level of the SOD activity and the MDA content were found in the focus center areas. Between non-MGC (groups A-D) and gastric ulcer (group F), the differences of SOD activity and MDA content were very noticeable in the gastric tissues from the focus peripheral and far-end areas, in which the SOD activity showed noticeable increase and the MDA content showed noticeable decrease in the gastric ulcer. CONCLUSION: The active free radical reaction in the gastric tissues can induce the carcinogenesis of non-MGC. The utmost low ability of antioxidation in the gastric tissues can induce the carcinogenesis of MGC. The metabolic change of the free radicals centralized mostly in the center of ulcerated lesions only, which suggested the ability of antioxidation was declined only in these lesions. However, the metabolism of free radicals varied significantly and the ability of antioxidation declined not only in the local focus area but also in the abroad gastric tissues with gastric carcinoma.


Assuntos
Carcinoma Papilar/metabolismo , Malondialdeído/metabolismo , Neoplasias Gástricas/metabolismo , Superóxido Dismutase/metabolismo , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Adenocarcinoma Mucinoso/metabolismo , Adenocarcinoma Mucinoso/patologia , Adulto , Idoso , Carcinoma Papilar/patologia , Carcinoma de Células em Anel de Sinete/metabolismo , Carcinoma de Células em Anel de Sinete/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Gástricas/patologia , Úlcera Gástrica/metabolismo , Úlcera Gástrica/patologia , Superóxidos/metabolismo
10.
Acta Crystallogr C ; 60(Pt 4): m170-1, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15071207

RESUMO

The title compound, [Ag(2)(C(7)H(4)ClO(2))(2)(C(5)H(6)N(2))(2)], lies about an inversion centre and the Ag atom is three-coordinated by two O atoms and one N atom from three different ligands. The 4-chlorobenzoate anion acts as a monodonor ligand, bridging two inversion-related Ag atoms of the compound into a dimer. There are weak intermolecular N-H.O hydrogen bonds in the structure.

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