Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 82
Filtrar
1.
Cell Biol Int ; 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34747545

RESUMO

Androgenetic alopecia (AGA) is the most common pattern of hair loss resulting from the effects of androgen on hair follicle. MicroRNAs (miRs) serve imperative roles in the regulation of many biological processes of hair follicle. However, the exact molecular mechanism of AGA remains to be elucidated. In the present study, we found miR-122, which is mainly recognized as a tumor suppressor, was highly overexpressed in the bulb of balding HFs in comparison with non-balding ones in AGA. Moreover, miR-122 induces apoptosis of human dermal papilla cells (hDPCs) with miR-122 mimics in vitro, and the expression of insulin-like growth factor 1 receptor (IGF1R) in hDPCs was reduced following upregulation of miR-122. Mechanistically, dual luciferase reporter assay confirmed that miR-122 directly targeted the 3'-untranslated region of IGF1R.These findings suggested that upregulation of miR-122 induces apoptosis, potentially via the repression of IGF1R in hDPCs of AGA, providing a novel insight into the potential pathological mechanism of miR-122 in AGA DPCs. This article is protected by copyright. All rights reserved.

2.
J Mater Chem B ; 2021 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-34842263

RESUMO

As an incurable metabolic disease, gouty arthritis (GA) requires long-term treatment with frequent drug administration several times per day. Compared to non-specific small organic medications, interleukin-1ß (IL-1ß) blocking therapies, such as IL-1 receptor antagonist (IL-1Ra), show great therapeutic potential in clinical trials of GA. However, IL-1Ra application is starkly limited due to its short half-life and poor bioavailability. Herein, we demonstrate a new type of nanotherapeutic formulation via noncovalent assembly of an engineered IL-1Ra chimera protein. PEGylation was employed to induce such assembly by exploiting electrostatic complexation and hydrophobic interactions. The engineered protein nanoparticles had a combination of biocompatibility, improved bioavailability and therapeutic performance. It showed extraordinary long-term anti-inflammatory effect and robust bio-efficacy for GA therapy in acute GA rat models. Strikingly, this nanoprotein system possesses an ultralong half-life of 27 hours and a bioavailability 7 times higher than that of pristine IL-1Ra, thus extending the dosing interval from several hours to more than 3 days. Therefore, our noncovalent assembly strategy via an engineered chimeric protein empowers the construction of potent delivery nanosystems for efficient GA treatment, and this might be adapted for other therapeutics to form long-acting formulations.

3.
Front Cell Dev Biol ; 9: 728188, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34722509

RESUMO

Hair follicle stem cells are extensively reprogrammed by the aging process, manifesting as diminished self-renewal and delayed responsiveness to activating cues, orchestrated by both intrinsic microenvironmental and extrinsic macroenvironmental regulators. Dermal white adipose tissue (dWAT) is one of the peripheral tissues directly adjacent to hair follicles (HFs) and acts as a critical macroenvironmental niche of HF. dWAT directly contributes to HF aging by paracrine signal secretion. However, the altered interrelationship between dWAT and HF with aging has not been thoroughly understood. Here, through microdissection, we separated dWAT from the skin of aged mice (18 months) and young mice (2 months) in telogen and depilation-induced anagen for transcriptome comparing. Notably, compared with young dWAT, aberrant inflammatory regulators were recapitulated in aging dWAT in telogen, including substantial overexpressed inflammatory cytokines, matrix metalloproteinases, and prostaglandin members. Nonetheless, with anagen initiation, inflammation programs were mostly abolished in aging dWAT, and instead of which, impaired collagen biosynthesis, angiogenesis, and melanin synthesis were identified. Furthermore, we confirmed the inhibitory effect on hair growth of CXCL1, one of the most significantly upregulated inflammation cytokines in aging dWAT. Besides this, we also identified the under-expressed genes related to Wnt signaling fibroblast growth factor family members and increased BMP signaling in aging dWAT, further unraveling the emerging role of dWAT in aging HFs malfunction. Finally, we proved that relieving inflammation of aging dWAT by injecting high-level veratric acid stimulated HF regenerative behavior in aged mice. Concomitantly, significantly decreased TNF-a, CCL2, IL-5, CSF2, and increased IL10 in dWAT was identified. Overall, the results elaborated on the complex physiological cycling changes of dWAT during aging, providing a basis for the potential regulatory effect of dWAT on aging HFs.

4.
Front Cell Dev Biol ; 9: 724310, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34604224

RESUMO

Dermal papillae are a target of androgen action in patients with androgenic alopecia, where androgen acts on the epidermis of hair follicles in a paracrine manner. To mimic the complexity of the dermal papilla microenvironment, a better culture model of human dermal papilla cells (DPCs) is needed. Therefore, we evaluated the inhibitory effect of dihydrotestosterone (DHT)-treated two-dimensional (2D)- and 3D-cultured DPCs on hair follicle growth. 2D- and 3D-cultured DPC proliferation was inhibited after co-culturing with outer root sheath (ORS) cells under DHT treatment. Moreover, gene expression levels of ß-catenin and neural cell adhesion molecules were significantly decreased and those of cleaved caspase-3 significantly increased in 2D- and 3D-cultured DPCs with increasing DHT concentrations. ORS cell proliferation also significantly increased after co-culturing in the control-3D model compared with the control-2D model. Ki67 downregulation and cleaved caspase-3 upregulation in DHT-treated 2D and 3D groups significantly inhibited ORS cell proliferation. Sequencing showed an increase in the expression of genes related to extracellular matrix synthesis in the 3D model group. Additionally, the top 10 hub genes were identified, and the expression of nine chemokine-related genes in DHT-treated DPCs was found to be significantly increased. We also identified the interactions between transcription factor (TF) genes and microRNAs (miRNAs) with hub genes and the TF-miRNA coregulatory network. Overall, the findings indicate that 3D-cultured DPCs are more representative of in vivo conditions than 2D-cultured DPCs and contribute to our understanding of the molecular mechanisms underlying androgen-induced alopecia.

5.
J Pept Sci ; : e3365, 2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34467600

RESUMO

Long-chain scorpion toxin AaH-II isolated from Androctonus australis Hector can selectively inhibit mammalian voltage-gated sodium ion channel Nav 1.7 responsible for pain sensation. Efficient chemical synthesis of AaH-II and its derivatives is beneficial to the study of the function and mechanism of Nav 1.7 and the development of potential peptide inhibitors. Herein, we compared three different strategies, namely, direct solid-phase peptide synthesis, hydrazide-based two-segment native chemical ligation, and hydrazide-based three-segment native chemical ligation for the synthesis of AaH-II. The hydrazide-based two-segment native chemical ligation affords the target toxin with the optimal efficiency, which provides a practically robust procedure for the preparation of tool molecules derived from AaH-II to study the biological functions and modulation of Nav 1.7. Our work highlights the importance of selecting suitable segment condensation approach in the chemical synthesis of protein toxins.

6.
Int J Biol Macromol ; 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34509522

RESUMO

Hair loss remains a challenging clinical problem that influences the quality of life. Three-dimensional (3D) bioprinting has become a valuable tool for fabricating tissue constructs for transplantation and other biomedical applications. Although some simple organs, such as skin and cartilage, have been successfully simulated, it remains challenging to make hair follicles (HFs), which are highly complex organs. The tissue engineering of human HFs has been a long-standing challenge, and progress with this has lagged behind that with other lab-grown tissues. This is principally due to a lack of availability of a platform that can successfully recapitulate the microenvironmental cues required to maintain the requisite cellular interactions for hair neogenesis. In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multilayer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body. This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo. In conclusion, our 3D-bioprinted multilayer composite scaffold prepared using a gelatin/alginate hydrogel provides a suitable 3D microenvironment for DPCs to induce HF formation. The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss. This method may also have critical applications for skin tissue engineering, with its appendages, for other purposes.

7.
Sci Total Environ ; 797: 149015, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34346373

RESUMO

Over 40% of herbicides used today are chiral. Dichlorprop (2, 4-DCPP) is a widely used typical broad-spectrum chiral aryloxyphenoxy propionic acid (AOPP) herbicide. However, the molecular mechanism of the enantioselectivity of DCPP enantiomers (S-DCPP and R-DCPP) and their effects on non-target organisms are remain unclear. In the present study, the model plant Arabidopsis thaliana was treated by DCPP enantiomers to directly reveal the effects of DCPP enantiomers on plant growth, as well as metabolic profile. Results showed that the enantioselectivity embodied in that R-DCPP treatment led to the decrease of shoot weight, the significantly variation on morphology of shoot and root, oxidative damage, et al., while the plant morphology also changes to a certain extent associated oxidative damage after treated by S-DCPP. By using metabolomic analysis, it was found that R-DCPP had significant effects on A. thaliana leaf metabolism, including lactose metabolism, starch and sucrose metabolism, TCA cycle, fatty acid biosynthesis pathway and pentose phosphate pathway, and accumulated a lot of antioxidants in plant leaves, while the amino acids and some terpenoids increased in S-DCPP group. Our study provides a new direction to explore the relationship between chiral herbicides on leaf metabolism, and the effect of this relationship on the plant growth.


Assuntos
Arabidopsis , Herbicidas , Ácido 2,4-Diclorofenoxiacético/análogos & derivados , Herbicidas/toxicidade , Folhas de Planta , Estereoisomerismo
8.
Indian J Microbiol ; 61(3): 338-347, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34290462

RESUMO

Gut microbiome, as the largest and most important micro-ecosystem, plays a critical role in health. The purpose of this study was to evaluate whether heat stress modulates the composition and diversity of the gut microbiome in rats. The heat stress model was prepared in rats with the heating temperature maintained at 35-38°C. Cecum contents were collected after heat stress for 3 h and days 1, 3 and 7. Total DNA was extracted for 16 S rRNA sequencing and analysis of intestinal microbiome composition and diversity. The study showed that the composition of the intestinal microbiome of heat stress group was changed. And the heat stress modulated key phylotypes of gut microbiota at the level of phylum and genus. In particular, the genus of Lactobacillus and Bacteroides were significantly reduced, whereas the Oscillospira and Clostridium were increased by heat stress. Meanwhile, the rats under the heat stress encountered the change in carbohydrate metabolism, amino acid metabolism, and membrane transport to defense against stress. Taken together, the composition and structure of gut microbiome were affected by heat stress and some key phylotypes were also significantly altered. We conclude that the heat stress could impact multiple biological functions, via altering the gut microbiome.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34331648

RESUMO

A new Fe-C porous filter material was prepared with dredged sediment of river as raw material. The orthogonal test L9(34) and component ratio experiment of raw material were conducted to investigate the optimum technological condition. Further, the filter obtained was characterized by Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), energy dispersive X-ray spectroscope (EDS), and X-ray diffraction (XRD). Results showed that the optimal technological condition was sludge: straw: starch: iron powder: foam: iron powder 74.5: 10: 7.5: 3: 5, preheating temperature 280 °C, preheating 15 min, sintering temperature 1080 °C, and sintering 11 min. The BET surface area of the filter was 3.32 m2 g-1, and average pore size was 10.05 nm. Phase composition mainly included SiO2, Fe3O4, Fe2O3, and muscovite (KAl2(Si3Al)O10(OH)2). Average effluent concentrations of total phosphorus (TP), total organic carbon (TOC), and total nitrogen (TN) of the biofilter system filled with the filter obtained were decreased to 0.08, 3.43, and 3.76 mg L-1, separately, at hydraulic retention time 4 h. Thus, the filter prepared with dredged sediment of rive as raw material is an alternative material for polluted river water purification.

10.
Sci Total Environ ; 797: 149200, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34303973

RESUMO

Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials in industrial and agricultural production. Glyphosate is a broad-spectrum systemic herbicide, which mainly acts in the phloem of weeds that compete with crop growth and is widely used in agriculture. This study investigated the interactive effects of AgNPs and glyphosate on the physiological morphology, gene transcription, and rhizosphere microorganisms of wheat. Our results demonstrated that wheat growth, and the structure and diversity of rhizosphere microorganisms were slightly influenced by AgNPs and glyphosate single treatment at the test concentration. However, AgNPs and glyphosate (Gly) combined treatment (AgNPs + Gly) strongly inhibited wheat growth and influenced gene transcription. In total, 955, 601, and 1336 genes were determined to be differentially expressed in AgNPs, glyphosate, and combined treatment, respectively. According to KEGG analysis, the combined groups induced an antioxidant response by upregulating the transcription of phenylpropanoid biosynthesis-related genes. In addition, more energy was needed, and disrupted cell membrane was shown in the combined treatment, which displayed in the upregulation of sucrose, starch, and lipid synthesis. Moreover, the relative abundance of Bradyrhizobium, Devosia, Kribbella, Sphingopyxis (nitrogen-fixing bacteria), and Streptomyces (plant growth-promoting bacteria) in soil microbiota were decreased, implicated that nitrogen fixation and some beneficial substance secretions were inhibited by the combined treatment. This study emphasized that the synergetic effects of AgNPs and glyphosate exerted a negative impact on wheat growth.


Assuntos
Nanopartículas Metálicas , Prata , Glicina/análogos & derivados , Nanopartículas Metálicas/toxicidade , Rizosfera , Prata/toxicidade , Microbiologia do Solo , Triticum
11.
Microb Biotechnol ; 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-34180582

RESUMO

Staphylococcus xylosus (S. xylosus)-induced cow mastitis is an extremely serious clinical problem. However, antibiotic therapy does not successfully treat S. xylosus infection because these bacteria possess a strong biofilm formation ability, which significantly reduces the efficacy of antibiotic treatments. In this study, we developed ceftiofur-loaded chitosan grafted with ß-cyclodextrins (CD-g-CS) nanoparticles (CT-NPs) using host-guest interaction. These positively charged nanoparticles improved bacterial internalization, thereby significantly improving the effectiveness of antibacterial treatments for planktonic S. xylosus. Moreover, CT-NPs effectively inhibited biofilm formation and eradicated mature biofilms. After mammary injection in a murine model of S. xylosus-induced mastitis, CT-NPs significantly reduced bacterial burden and alleviated inflammation, thereby achieving optimized therapeutic efficiency for S. xylosus infection. In conclusion, this treatment strategy could improve the efficiency of antibiotic therapeutics and shows great potential in the treatment of S. xylosus infections.

12.
Angew Chem Int Ed Engl ; 60(31): 17171-17177, 2021 07 26.
Artigo em Inglês | MEDLINE | ID: mdl-34021957

RESUMO

Activity-based E2 conjugating enzyme (E2)-ubiquitin (Ub) probes have recently emerged as effective tools for studying the molecular mechanism of E3 ligase (E3)-catalyzed ubiquitination. However, the preparation of existing activity-based E2-Ub probes depends on recombination technology and bioconjugation chemistry, limiting their structural diversity. Herein we describe an expedient total chemical synthesis of an E2 enzyme variant through a hydrazide-based native chemical ligation, which enabled the construction of a structurally new activity-based E2-Ub probe to covalently capture the catalytic site of Cys-dependent E3s. Chemical cross-linking coupled with mass spectrometry (CXMS) demonstrated the utility of this new probe in structural analysis of the intermediates formed during Nedd4 and Parkin-mediated transthiolation. This study exemplifies the utility of chemical protein synthesis for the development of protein probes for biological studies.


Assuntos
Compostos de Sulfidrila/metabolismo , Ubiquitina-Proteína Ligases/análise , Ubiquitina/química , Biocatálise , Humanos , Estrutura Molecular , Compostos de Sulfidrila/química , Ubiquitina/síntese química , Ubiquitina-Proteína Ligases/metabolismo
13.
Front Cell Dev Biol ; 9: 661116, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33859987

RESUMO

Dental pulp stem cell (DPSC) transplantation has shown new prospects in dental pulp regeneration, and is of great significance in the treatment of pulpitis and pulp necrosis. The fate and regenerative potential of stem cells are dependent, to a great extent, on their microenvironment, which is composed of various tissue components, cell populations, and soluble factors. N-cadherin-mediated cell-cell interaction has been implicated as an important factor in controlling the cell-fate commitment of mesenchymal stem cells. In this study, the effect of N-cadherin on odontogenic differentiation of DPSCs and the potential underlying mechanisms, both in vitro and in vivo, was investigated using a cell culture model and a subcutaneous transplantation mouse model. It was found that the expression of N-cadherin was reversely related to the expression of odontogenic markers (dentin sialophosphoprotein, DSPP, and runt-related transcription factor 2, Runx2) during the differentiation process of DPSCs. Specific shRNA-mediated knockdown of N-cadherin expression in DPSCs significantly increased the expression of DSPP and Runx2, alkaline phosphatase (ALP) activity, and the formation of mineralized nodules. Notably, N-cadherin silencing promoted nucleus translocation and accumulation of ß-catenin. Inhibition of ß-catenin by a specific inhibitor XAV939, reversed the facilitating effects of N-cadherin downregulation on odontogenic differentiation of DPSCs. In addition, knockdown of N-cadherin promoted the formation of odontoblast-like cells and collagenous matrix in ß-tricalcium phosphate/DPSCs composites transplanted into mice. In conclusion, N-cadherin acted as a negative regulator via regulating ß-catenin activity during odontogenic differentiation of DPSCs. These data may help to guide DPSC behavior by tuning the N-cadherin-mediated cell-cell interactions, with implications for pulp regeneration.

14.
J Hazard Mater ; 411: 125137, 2021 06 05.
Artigo em Inglês | MEDLINE | ID: mdl-33858101

RESUMO

S-metolachlor (S-ME) is a common chloroacetanilide herbicide. Here, we investigated the effects of S-ME on wheat seedling growth and explored via metabolomics the driver through which S-ME changes the rhizosphere microbiome. The results indicated that 4 mg/kg S-ME had a strong inhibitory effect on plant growth by inducing hydrogen peroxide (H2O2) levels. The richness of the rhizosphere microbiome markedly decreased after S-ME treatment, although the abundance of some potential beneficial rhizobacteria, such as Rhizobiaceae and Burkholderiaceae, increased suggesting that plants recruited potential beneficial microorganisms to resist S-ME-induced stress. Spearman correlation analysis revealed that Rhizobiaceae and Burkholderiaceae were positively correlated with organic acids secreted by plants after S-ME treatment, implying that potential beneficial microorganisms may be attracted mainly by organic acids. Our results demonstrated the phytotoxicity of S-ME on crop growth and indicated both that S-ME could influence rhizosphere microorganism abundance and that recruitment of potential beneficial microorganisms could be the result of root exudate regulation.


Assuntos
Microbiota , Rizosfera , Acetamidas , Exsudatos e Transudatos , Peróxido de Hidrogênio , Raízes de Plantas , Plântula , Microbiologia do Solo , Triticum
15.
J Nanobiotechnology ; 19(1): 94, 2021 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-33789665

RESUMO

BACKGROUND: Periodically regenerated hair follicles provide an excellent research model for studying tissue regeneration and stem cell homeostasis. Periodic activation and differentiation of hair follicle stem cells (HFSCs) fuel cyclical bouts of hair regeneration. HFSCs represent an excellent paradigm for studying tissue regeneration and somatic stem cell homeostasis. However, these crucial studies are hampered by the lack of a culture system able to stably expand human HFSCs and regulate their fate. RESULTS: Here, we use layer-by-layer (LbL) self-assembly with gelatin/alginate to construct a nanoscale biomimetic extracellular matrix (ECM) for an HFSC population. The LbL coating provides ECM and mechanical support for individual cells, which helps to maintain the CD200+α6+ HFSC population to a certain extent. Addition of key signal molecules (FGF-7 and VEGF-A) simulates the minimum essential components of the stem cell microenvironment, thereby effectively and stably expanding HFSCs and maintaining the CD200+α6+ HFSC population. Subsequently, BMP2 loaded to the nanocoated layer, as a slow-release signal molecule, activates BMP signaling to regulate HFSCs' fate in order to obtain a purified CD200+α6+ HFSC population. CONCLUSION: This system can minimize the microenvironment of HFSCs; thus, stably amplifying HFSCs and revealing their plasticity. Our study thus provides a new tool for studies of hair follicle reconstruction and stem cell homeostasis.


Assuntos
Folículo Piloso/fisiologia , Nanotecnologia/métodos , Células-Tronco/fisiologia , Engenharia Tecidual/métodos , Alginatos , Proteína Morfogenética Óssea 2 , Proliferação de Células , Sobrevivência Celular , Gelatina , Cabelo , Folículo Piloso/citologia , Homeostase , Humanos , Transdução de Sinais , Células-Tronco/citologia
16.
Microbiol Res ; 248: 126743, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33713869

RESUMO

The interaction between plants and microorganisms directly affects plant health and sustainable agricultural development. Leaves represent a wide-area habitat populated by a variety of microorganisms, whose impact on host environmental adaptability could influence plant growth and function. The driving factors for phyllosphere microbiota assemblage are the focus of current research. Here, we investigated the effect of growth stage (i.e., bolting, flowering, and maturation) and genotype of Arabidopsis thaliana (wild-type and the two photosynthetic mutants ndf4 and pgr5) on the composition of phyllosphere microbiota. Our results show that species abundance varied significantly between the three genotypes at different growth stages, whereas species richness and evenness varied only for ndf4. The leaf surface shared a core microbiota dominated by Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes in all tested growth stages and genotypes. Phyllosphere specificity varied more with respect to growth stage than to genotype. In summary, both the growth stage and genotype of A. thaliana are crucial in shaping phyllosphere bacterial composition, with the former being a stronger driver. Our findings provide a novel for investigating whether the host properties influence the phyllosphere community and favor healthy development of plants.


Assuntos
Arabidopsis/crescimento & desenvolvimento , Arabidopsis/microbiologia , Bactérias/isolamento & purificação , Microbiota , Arabidopsis/genética , Bactérias/classificação , Bactérias/genética , Genótipo , Filogenia , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/microbiologia , Microbiologia do Solo
17.
J Cosmet Dermatol ; 20(10): 3227-3235, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33752252

RESUMO

BACKGROUND: Platelet rich plasma (PRP) has been accepted as a potential therapy for treating androgenetic alopecia (AGA). OBJECTIVE: To fully clarify the underling molecular mechanisms of PRP action on hair growth and promote its clinical applications. METHODS: In this study, we used mice models and protein biochip to explore the specific mechanisms of PRP regulating hair growth. Then, we performed a randomized, placebo-controlled, double-blind, half-head study of 52 AGA patients to verify the therapeutic efficacy of PRP in Chinese AGA patients. RESULTS: The results confirmed that PRP treatment boosted hair regrowth, accelerated hair cycling, and the effect sustained for more than one hair cycle in mice. Protein biochip evaluation confirmed remarkably upregulated ß-Catenin, PDGF, and AKT signaling and repressed p53 signaling in PRP injection group. Clinically, mean hair count, density, diameter, and anagen hair ratio in PRP group showed a significant improvement at 6 month comparing to control side. CONCLUSIONS: Overall, we elucidated the specific molecular mechanism of PRP action on hair growth and proved the therapeutic efficacy and safety of PRP in Chinese AGA patients.


Assuntos
Alopecia , Plasma Rico em Plaquetas , Alopecia/terapia , Animais , Método Duplo-Cego , Cabelo , Humanos , Camundongos , Transdução de Sinais , Resultado do Tratamento
19.
Biomed Pharmacother ; 137: 111247, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33517191

RESUMO

Androgenic alopecia (AGA), also known as male pattern baldness, is one of the most common hair loss diseases worldwide. The main treatments of AGA include hair transplant surgery, oral medicines, and LDL laser irradiation, although no treatment to date can fully cure this disease. Animal models play important roles in the exploration of potential mechanisms of disease development and in assessing novel treatments. The present study describes androgen receptor (AR) in C57BL/6 mouse hair follicles that can be activated by dihydrotestosterone (DHT) and translocate to the nucleus. This led to the design of a mouse model of androgen-induced AGA in vivo and in vitro. DHT was found to induce early hair regression, hair miniaturization, hair density loss, and changes in hair morphology in male C57BL/6 mice. These effects of DHT could be partly reversed by the AR antagonist bicalutamide. DHT had similar effects in an ex vivo model of hair loss. Evaluation of histology, organ culture, and protein expression could explain the mechanism by which DHT delayed hair regrowth.


Assuntos
Alopecia/metabolismo , Di-Hidrotestosterona , Folículo Piloso/metabolismo , Receptores Androgênicos/metabolismo , Alopecia/induzido quimicamente , Alopecia/tratamento farmacológico , Alopecia/fisiopatologia , Antagonistas de Androgênios/farmacologia , Anilidas/farmacologia , Animais , Modelos Animais de Doenças , Folículo Piloso/efeitos dos fármacos , Folículo Piloso/crescimento & desenvolvimento , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Nitrilas/farmacologia , Técnicas de Cultura de Órgãos , Transdução de Sinais , Compostos de Tosil/farmacologia
20.
Sci Total Environ ; 769: 145156, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33477045

RESUMO

Agricultural chemicals have the potential to become pollutants that adversely affect plant growth. Interactions between these compounds are likely, but potential synergies are under-researched. Multiwall carbon nanotubes are increasingly finding novel uses in agriculture, as delivery mechanisms and as slow-release fertilizers. There is potential for nanotubes to interact with other agricultural chemicals in unpredictable ways. To investigate this possibility, we examined interactions with glyphosate, a widely used herbicide that is also attracting increasing concern over its potential for non-target effects. Here we examined potential synergistic effects on hydroponically grown Arabidopsis thaliana. Single treatments did not affect plant growth significantly, or did only mildly. However, combined treatment significantly affected both plant root and shoot growth. High-level content of malondialdehyde and up-regulated of metabolic antioxidant molecules in plant indicated that combined group caused the strong oxidative damage, while the decreased of antioxidant enzyme activities indicated an imbalance between reactive oxygen species (ROS)and the antioxidant defense system due to the continuously generated ROS. Besides, several intermediate metabolites of unsaturated fatty acids synthesis pathways were up-regulated in combined treatment, which clarified that combined group changed membrane components. The increase of intermediate metabolites in combined group also reflected more energy consumption in the repairment of the disrupt of combined treatment. The synergistic effect observed was attributed to the accumulation of glyphosate resulting from permeability and transportability of the carbon nanotubes. Overall, the risk of nanotube-herbicide interaction suggests a caution use of nanotubes in agricultural applications.


Assuntos
Arabidopsis , Nanotubos de Carbono , Glicina/análogos & derivados , Glicina/toxicidade , Nanotubos de Carbono/toxicidade , Estresse Oxidativo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...