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1.
Braz Dent J ; 31(1): 63-68, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32159708

RESUMO

The present study evaluated polymorphisms in RANK, RANKL and OPG-encoding genes to assess whether they are associated with mucositis and peri-implantitis in a population from the Brazilian Amazon region. One hundred and fourteen patients with dental implants were included in the study. After clinical and radiographic examination, the sample was categorized into 4 groups, according to the peri-implant status: Healthy (n=71), Mucositis (n=30), Peri-implantitis (n=13) and Diseased (Mucositis + Peri-implantitis, n=43). Genomic DNA was extracted from buccal cells from saliva, and the genetic polymorphism in osteoprotegerin (OPG), Kappa nuclear factor activator receptor (RANKL) and nuclear kappa factor activator receptor (RANK) were genotyped by the real time PCR. Univariate and multivariate statistical analyses were performed to compare clinical variables among groups and to evaluate genotypes and alleles distributions and the established alpha was 5%. Age, peri-implant biotype, diabetes and presence of peri-implant biofilm were associated with mucositis (p<0.05) and peri-implantitis (p<0.05). Smoking, alcoholism, and periodontal biofilms were also associated with the presence of peri-implantitis (p<0.05). Univariate and multivariate analysis did not demonstrate an association of peri-implantitis or mucositis with any genetic polymorphism in RANK (rs3826620), RANKL (rs9594738) and OPG (rs2073618) (p>0.05). The studied genetic polymorphism in RANK, RANKL and OPG were not associated with mucositis and peri-implantitis in a Brazilian population from the Amazon region.


Assuntos
Implantes Dentários , Osteoprotegerina/genética , Peri-Implantite , Ligante RANK/genética , Receptor Ativador de Fator Nuclear kappa-B/genética , Brasil , Humanos , Mucosa Bucal , Polimorfismo Genético
2.
Braz. dent. j ; 31(1): 63-68, Jan.-Feb. 2020. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1089266

RESUMO

Abstract The present study evaluated polymorphisms in RANK, RANKL and OPG-encoding genes to assess whether they are associated with mucositis and peri-implantitis in a population from the Brazilian Amazon region. One hundred and fourteen patients with dental implants were included in the study. After clinical and radiographic examination, the sample was categorized into 4 groups, according to the peri-implant status: Healthy (n=71), Mucositis (n=30), Peri-implantitis (n=13) and Diseased (Mucositis + Peri-implantitis, n=43). Genomic DNA was extracted from buccal cells from saliva, and the genetic polymorphism in osteoprotegerin (OPG), Kappa nuclear factor activator receptor (RANKL) and nuclear kappa factor activator receptor (RANK) were genotyped by the real time PCR. Univariate and multivariate statistical analyses were performed to compare clinical variables among groups and to evaluate genotypes and alleles distributions and the established alpha was 5%. Age, peri-implant biotype, diabetes and presence of peri-implant biofilm were associated with mucositis (p<0.05) and peri-implantitis (p<0.05). Smoking, alcoholism, and periodontal biofilms were also associated with the presence of peri-implantitis (p<0.05). Univariate and multivariate analysis did not demonstrate an association of peri-implantitis or mucositis with any genetic polymorphism in RANK (rs3826620), RANKL (rs9594738) and OPG (rs2073618) (p>0.05). The studied genetic polymorphism in RANK, RANKL and OPG were not associated with mucositis and peri-implantitis in a Brazilian population from the Amazon region.


Resumo O presente estudo avaliou a associação da predisposição clínica e dos fatores genéticos com a presença de doenças peri-implantares. Cento e quatorze pacientes com implantes dentais instalados na Clínica de Especialização do Amazonas, Brazil, foram incluidos no estudo. Após exame clínico e radiográfico, a amostra foi categorizada em 4 grupos, de acordo com o Status peri-implantar: saúde (n=71), mucosite (n=30), peri-implantite (n=13) e doentes (mucosite + peri-implantite). DNA genômico foi extraído de células orais da saliva, e o polimorfismo genético em osteoprotegerina (OPG), ligante do receptor ativador do fator Kappa nuclear (RANKL) e receptor ativador do fator Kappa nuclear (RANK) foram genotipados por PCR em tempo real. O estudo se propôs a avaliar se os polimorfismos em RANK, RANKL e OPG estão envolvidos na patogênese da mucosite e da peri-implantite, e avaliar também a presença de fatores de risco moduladores da resposta em uma população brasileira. Idade, biotipo peri-implantar, diabetes e presença de biofilme peri-implantar foram associados a mucosite (p<0.05) e peri-implantite (p<0.05). Tabagismo, alcoolismo e biofilme periodontal também foram associados com a presença de peri-implantite (p<0.05). Análise univariada e multivariada não demonstraram associação de peri-implantite ou mucosite com os polimorfismos genéticos em RANK (rs3826620), RANKL (rs9594738) e OPG (rs2073618) (p>0.05). Os polimorfismos genéticos estudados não foram associados com mucosite e peri-implantite em uma população brasileira da região Amazônica.

3.
Rev. Cient. CRO-RJ (Online) ; 4(2): 60-66, May-Aug. 2019.
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1024938

RESUMO

Introduction: Robinow syndrome is a rare genetic disorder of skeletal development. It is characterized by short stature, facial dysmorphisms and orodental anomalies, underdeveloped genitalia, mesomelic brachymelia. Case Report: A 6 year old girl with Robinow Syndrome was referred in the Center for Formation of Human Resources Specialized in Dental Care to Special Needs Patients (Ribeirão Preto, São Paulo, Brazil) for evaluation. Medical history investigation and clinicalexamination were observed in short stature, facial dimorphism and hypoplastic genitalia. Orofacial findings showed retrognathism, a mid-line hemangioma approximately 1 cm wide with upturned borders, bifid tongue, ankyloglossia, general gingival hypertrophy, deep bite and mild tooth crowding. Cardiac abnormality and neuromotor developmental delay consisted of systemic manifestations present which demanded individualized dental care. The dental treatment consisted of preventive and restoratives procedures to adequate the oral health condition of the patient and orthodontic treatmentwas planned. The patient has been in follow-up for six years. Conclusion: Orofacial anomalies andother alterations found in the present case contributed to complement the orofacial findings described in the literature and to assist in diagnosis of the syndrome. In this case, amultiprofessional team and integral treatment were essential to rescue oral health and improvelife quality of the patient.


Introdução: A síndrome de Robinow é uma doença genética rara caracterizada por baixa estatura, dismorfismos faciais e anomalias orodentais, genitália subdesenvolvida e falhas no desenvolvimento esquelético. Relato de Caso: Uma paciente de 6 anos com Síndrome de Robinow foi encaminhada ao Centro de Formação de Recursos Humanos Especializado no Atendimento Odontológicode Pacientes com Necessidades Especiais (Ribeirão Preto, São Paulo, Brasil). Na investigação da história médica e ao exame clínico foram observados baixa estatura, dismorfismo facial e genitália hipoplásica. Os achados orofaciais mostraram retrognatismo, hemangioma de linha média de aproximadamente 1 cm de largura com bordas voltadas para cima, língua bífida, anquiloglossia, hipertrofia gengival geral, mordida profunda e apinhamento dentário leve. A anormalidade cardíaca e o atraso no desenvolvimento neuromotor demandaram cuidados individualizados. O tratamento odontológico consistiu em procedimentos preventivos e restauradores para adequar a condição de saúde bucal da paciente. Além disso, foi realizada avaliação ortodôntica e planejamento do tratamento. A paciente está em acompanhamento há seis anos. Conclusão: Anomalias orofaciais e outras alterações encontradas no presente caso contribuíram para complementar os achados orofaciais na literatura e auxiliar no diagnóstico da síndrome. Neste caso, um plano de tratamento integral e equipe multiprofissional foram essenciais para resgatar a saúde bucal e propiciar melhor qualidade de vida à paciente.


Assuntos
Saúde Bucal , Equipe de Assistência ao Paciente , Retrognatismo , Criança , Assistência Odontológica
4.
Braz Dent J ; 30(2): 123-132, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30970054

RESUMO

The aim of this study was to evaluate the radiographic periapical repair and the synthesis of inflammatory mediators after endodontic treatment in a single session, using different irrigation protocols, in teeth with apical periodontitis. Experimental apical periodontitis were induced in dog's teeth randomly assigned into 4 groups: G1 - Irrigation by Negative Apical Pressure (n= 20); G2 - Passive Ultrasonic Irrigation (n= 20), G3 - Positive Pressure Irrigation (n= 20); G4 - apical periodontitis without treatment (n= 20). After 180 days, the animals were euthanized, the tissues removed and submitted to histotechnical processing for immunohistochemical analysis of osteopontin (OPN), tumor necrosis factor-a (TNF-a) and interleukin 1-a (IL-1a). Radiographic analysis was performed using the Periapical Index (PAI), obtained prior to and 180 days following endodontic treatment. Data were analyzed using Wilcoxon signed-rank test, Fisher's Exact test or Kruskal-Wallis test and Dunn's post-test (a = 5%). Radiographically, after endodontic treatment, apical periodontitis persisted in 35% of G1 specimens, 40% of G2 and 40% of G3 (p>0.05), although a PAI reduction was observed (p<0.05). By immunohistochemical evaluation, endodontic treatment resulted in lower synthesis of TNF-a and OPN in periapical region, compared to apical periodontitis without treatment (p<0.05). Production of IL-1 was not modulated by endodontic treatment (p>0.05). Periapical healing was observed in approximately 60% of the cases after endodontic treatment performed in a single session with lower synthesis of TNF-a and OPN in the periapical region, regardless of the irrigation protocol used.


Assuntos
Periodontite Periapical , Fotoquimioterapia , Dente , Animais , Cavidade Pulpar , Cães , Tratamento do Canal Radicular
5.
Rev. Cient. CRO-RJ (Online) ; 4(1): 61-72, Jan.-Apr. 2019.
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1024216

RESUMO

Objectives: To evaluate the inflammatory tissue response and matrix metalloproteinase (MMP)-2 and -9 expression in the pulp-dentin complex in response to RelyX TM Unicem (RU) and Ketac Cem TM Easymix (KC) cements. Methods : Class V cavities were prepared in 56 teeth from six dogs, and indirect pulp capping was performed using RU (n=20), KC (n=20), zinc oxide, and eugenol cement (control, n=16). At 7 and 70 days following indirect pulp capping, the animals were euthanized, and tissues were removed for histological evaluation. The distance from the cavity floor to the odontoblastic layer was measured, and the numbers of inflammatory cells, fibroblasts, and odontoblasts were counted in pulp tissue. MMP-2 and -9 expression levels were immunohistochemically assessed. Statistical analyses were performed for all experiments (significance level=5%). Results : The dentin remnant thickness between the cavity floor and the pulp chamber was similar for all materials, ranging from 469 to 739 µm (p>0.05). At 7 days, KC and RU induced a small inflammatory response in the pulp-dentin complex, similar to the control (p>0.05). At 70 days, RU induced a tissue response characterized by fewer odontoblasts and more mononuclear cells (p<0.05), whereas KC induced a response similar to the control (p>0.05). Lutingagents induced low levels of MMP-2 and MMP-9 expression, similar to the control (p>0.05). Conclusion : KC and RU luting agents are compatible materials for use in deep cavities close to dental pulp tissue, although RU led to a slightly diminished odontoblastic population with a higher percentage of mononuclear cells.


Objetivo : Avaliar a resposta tecidual inflamatória e a expressão de metaloproteinase de matriz (MMP) -2 e -9 no complexo polpa-dentina em resposta aos cimentos RelyX TM Unicem (RU) e Ketac Cem TM Easymix (KC). Métodos : Cavidades classe V foram preparadas em 56 dentes de seis cães, e capeamento pulpar indireto foi realizado com cimento de RU (n = 20), KC (n = 20), óxido de zinco e eugenol (controle, n = 16). Aos 7 e 70 dias após o capeamento pulpar indireto, os animais foram eutanasiados, e os tecidos foram removidos para avaliação histológica. A distância entre o assoalho da cavidade e a camada odontoblástica foi medida, e os números de células inflamatórias, fibroblastos e odontoblastos foram contados no tecido pulpar. Os níveis de expressão de MMP-2 e -9 foram avaliados por imuno-histoquímica. Análises estatísticas foram realizadas para todos os experimentos (nível de significância = 5%). Resultados : A espessura da dentina remanescente entre o assoalho da cavidade e a câmara pulpar foi semelhante para todos os materiais, variando de 469 a 739 µm (p> 0,05). Aos 7 dias, KC e RU induziram uma pequena resposta inflamatória no complexo polpa-dentina, semelhante ao controle (p> 0,05). Aos 70 dias, a RU induziu uma resposta tecidual caracterizada por menos odontoblastos e mais células mononucleares (p <0,05), enquanto o KC induziu uma resposta semelhante ao controle (p> 0,05). Os agentes cimentantes induziram baixos níveis de expressão de MMP-2 e MMP-9, semelhantes ao controle (p> 0,05). Conclusão : Os agentes cimentantes KC e RU são materiais compatíveis para uso em cavidades profundas próximas ao tecido da polpa dentária, embora a UR tenha levado a uma população odontoblástica levemente diminuída, com maior porcentagem de células mononucleares.


Assuntos
Cimentos de Resina , Metaloproteinases da Matriz , Polpa Dentária , Capeamento da Polpa Dentária
6.
Braz. dent. j ; 30(2): 123-132, Mar.-Apr. 2019. graf
Artigo em Inglês | LILACS-Express | ID: biblio-1001434

RESUMO

Abstract The aim of this study was to evaluate the radiographic periapical repair and the synthesis of inflammatory mediators after endodontic treatment in a single session, using different irrigation protocols, in teeth with apical periodontitis. Experimental apical periodontitis were induced in dog's teeth randomly assigned into 4 groups: G1 - Irrigation by Negative Apical Pressure (n= 20); G2 - Passive Ultrasonic Irrigation (n= 20), G3 - Positive Pressure Irrigation (n= 20); G4 - apical periodontitis without treatment (n= 20). After 180 days, the animals were euthanized, the tissues removed and submitted to histotechnical processing for immunohistochemical analysis of osteopontin (OPN), tumor necrosis factor-a (TNF-a) and interleukin 1-a (IL-1a). Radiographic analysis was performed using the Periapical Index (PAI), obtained prior to and 180 days following endodontic treatment. Data were analyzed using Wilcoxon signed-rank test, Fisher's Exact test or Kruskal-Wallis test and Dunn's post-test (a = 5%). Radiographically, after endodontic treatment, apical periodontitis persisted in 35% of G1 specimens, 40% of G2 and 40% of G3 (p>0.05), although a PAI reduction was observed (p<0.05). By immunohistochemical evaluation, endodontic treatment resulted in lower synthesis of TNF-a and OPN in periapical region, compared to apical periodontitis without treatment (p<0.05). Production of IL-1 was not modulated by endodontic treatment (p>0.05). Periapical healing was observed in approximately 60% of the cases after endodontic treatment performed in a single session with lower synthesis of TNF-a and OPN in the periapical region, regardless of the irrigation protocol used.


Resumo O objetivo deste estudo foi avaliar o reparo periapical e a síntese de mediadores inflamatórios após tratamento endodôntico em dentes de cães com lesão periapical, em sessão única, utilizando diferentes protocolos de irrigação. Lesões periapicais foram induzidas experimentalmente em dentes de cães e aleatoriamente divididas em 4 grupos: G1 - Irrigação por Pressão Apical Negativa (n = 20); G2 - Irrigação Ultrassônica Passiva (n = 20), G3 - Irrigação por Pressão Positiva (n = 20); G4 - Lesão periapical sem tratamento (n = 20). Após 180 dias, os animais foram eutanasiados, as peças removidas e submetidas ao processamento histotécnico para análise imunohistoquímica para osteopontina (OPN), fator de necrose tumoral-a (TNF-a) e interleucina 1-a (IL-1a). A análise radiográfica do reparo das lesões periapicais foi realizada por meio do Índice Periapical, obtido antes e 180 dias após o tratamento endodôntico. Os resultados obtidos foram submetidos à análise estatística por meio dos testes de sinais de Wilcoxon, Exato de Fisher ou Kruskal-Wallis seguido pelo pós-teste de Dunn (a = 5%). O exame radiográfico após o tratamento endodôntico, mostrou a persistência de áreas radiolúcidas periapicais e descontinuidade da lâmina dura em 35% dos espécimes do G1, 40% do G2 e 40% do G3, embora uma redução no PAI tenha sido observada (p<0,05). Pela análise imuno-histoquímica, o tratamento endodôntico resultou na menor síntese de TNF-a e de OPN na região periapical, comparativamente à lesão periapical sem tratamento (p<0,05). A produção de IL-1a não foi modulada pelo tratamento endodôntico (p>0,05). Reparo da lesão periapical foi observado em cerca de 60% dos casos após tratamento endodôntico realizado em sessão única e menor síntese de TNF-a e de OPN na região periapical, independente do protocolo de irrigação utilizado.

7.
Braz Oral Res ; 32: e121, 2018 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-30517430

RESUMO

The aim of this study was to evaluate the influence of radiation therapy on root canal sealer push-out bond strength (BS) to dentin and the sealer/dentin interface after different final irrigation solutions (NaOCl, EDTA, and chitosan). Sixty-four maxillary canines were distributed into two groups (n=30): non-irradiated and irradiated with 60 Gy. Canals were prepared with Reciproc-R50 and subdivided (n=10) for final irrigation (NaOCl, EDTA, chitosan) and filled. Three dentin slices were obtained from each root third. The first slice of each third was selected for BS evaluation, and the failure mode was determined by stereomicroscopy. SEM analysis of the sealer-dentin interface was performed in the remaining slices. Two-way ANOVA and Tukey's tests (α=0.05) were used. Lower BS (P<0.0001) was obtained after irradiation (2.07±0.79 MPa), regardless of the final irrigation solution used. The NaOCl group (P<0.001) had the lowest BS in the irradiated (1.68±0.72) and non-irradiated (2.39±0.89) groups, whereas the EDTA (irradiated: 2.14±0.77 and non-irradiated: 3.92±1.54) and chitosan (irradiated: 2.37±0.73 and non-irradiated: 3.51±1.47) groups demonstrated a higher BS (P<0.05). The highest values were observed in the coronal third (3.17±1.38) when compared to the middle (2.74±1.36) and apical ones (2.09±0.97)(P<0.0001). There were more cohesive failures and more gaps in irradiated specimens, regardless of the final solution. The present study showed that radiation was associated with a decrease in BS, regardless of the final solution used, whereas chitosan increased BS in teeth subjected to radiation therapy.


Assuntos
Colagem Dentária/métodos , Dentina/efeitos dos fármacos , Dentina/efeitos da radiação , Resinas Epóxi/efeitos da radiação , Materiais Restauradores do Canal Radicular/efeitos da radiação , Irrigantes do Canal Radicular/efeitos da radiação , Análise de Variância , Quitosana/química , Falha de Restauração Dentária , Ácido Edético/química , Resinas Epóxi/química , Neoplasias de Cabeça e Pescoço/radioterapia , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Reprodutibilidade dos Testes , Materiais Restauradores do Canal Radicular/química , Irrigantes do Canal Radicular/química , Hipoclorito de Sódio/química , Estatísticas não Paramétricas , Propriedades de Superfície/efeitos dos fármacos , Propriedades de Superfície/efeitos da radiação , Raiz Dentária/efeitos dos fármacos , Raiz Dentária/efeitos da radiação
8.
Braz Dent J ; 29(5): 465-468, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30517445

RESUMO

The aim of this study was to evaluate the association between timing of permanent tooth emergence with overweight and obesity in children from Brazilian Amazon region. The studied population consisted of 192 children, 09 to 12 year-old, from public schools at Manaus, Amazonas-Brazil. Clinical examination was performed and the tooth emergence was evaluated according to the number of permanent erupted teeth. Body mass index z-score was calculated. For the statistical analysis 'Overweight/obese' group was compared with 'Normal weight' group in a case to control ratio 1:2. The t-test based on age was used for means comparison between the groups. A linear regression analysis using age and gender as co-variants was used. The established alpha was 5%. One hundred twenty-seven children were classified as normal weight and 65 were classified as overweight/obese (49 were overweight and 16 were obese). Overweight/obesity condition was associated with the gender, in which boys had a higher chance to present higher weight conditions (OR=1.84; CI 95% 1.06-3.37; p=0.04). The mean number of permanent teeth was higher in the overweight/obesity group (p<0.001). Linear regression analysis demonstrated that nutritional status, gender and age were strongly associated with number of permanent erupted teeth (p<0.05). In conclusion, our study demonstrated that timing of permanent tooth emergence is associated with overweigh/obesity in children from Manaus, Brazil.


Assuntos
Dentição Permanente , Sobrepeso/fisiopatologia , Obesidade Pediátrica/fisiopatologia , Erupção Dentária/fisiologia , Índice de Massa Corporal , Brasil , Criança , Feminino , Humanos , Masculino
9.
Braz. dent. j ; 29(5): 465-468, Sept.-Oct. 2018. tab
Artigo em Inglês | LILACS-Express | ID: biblio-974180

RESUMO

Abstract The aim of this study was to evaluate the association between timing of permanent tooth emergence with overweight and obesity in children from Brazilian Amazon region. The studied population consisted of 192 children, 09 to 12 year-old, from public schools at Manaus, Amazonas-Brazil. Clinical examination was performed and the tooth emergence was evaluated according to the number of permanent erupted teeth. Body mass index z-score was calculated. For the statistical analysis 'Overweight/obese' group was compared with 'Normal weight' group in a case to control ratio 1:2. The t-test based on age was used for means comparison between the groups. A linear regression analysis using age and gender as co-variants was used. The established alpha was 5%. One hundred twenty-seven children were classified as normal weight and 65 were classified as overweight/obese (49 were overweight and 16 were obese). Overweight/obesity condition was associated with the gender, in which boys had a higher chance to present higher weight conditions (OR=1.84; CI 95% 1.06-3.37; p=0.04). The mean number of permanent teeth was higher in the overweight/obesity group (p<0.001). Linear regression analysis demonstrated that nutritional status, gender and age were strongly associated with number of permanent erupted teeth (p<0.05). In conclusion, our study demonstrated that timing of permanent tooth emergence is associated with overweigh/obesity in children from Manaus, Brazil.


Resumo O objetivo deste estudo foi avaliar se a erupção dentária está associada com sobrepeso/obesidade em crianças da região Amazônica brasileira. A população estudada foi composta por 192 crianças, de 9 a 12 anos, de escolas públicas de Manaus, Amazonas-Brasil. O exame clínico foi realizado e a erupção dentária foi avaliada de acordo com o número de dentes permanentes irrompidos. O índice de massa corporal escore-z foi calculado. Para a análise estatística, comparou-se o grupo "sobrepeso / obesidade" com o grupo "peso normal" em uma proporção de controle 1: 2. O teste t baseado na idade foi utilizado para comparação das médias entre os grupos. Uma análise de regressão linear usando idade e sexo como co-variáveis foi utilizada. O alfa estabelecido foi de 5%. Cento e vinte e sete crianças foram classificadas com peso normal e 65 foram classificados com sobrepeso / obesidade (49 com sobrepeso e 16 com obesidade). A condição de sobrepeso / obesidade associou-se ao gênero, no qual os meninos tiveram maior chance de apresentar condições de maior peso (OR = 1,84; IC 95% 1,06-3,37; p = 0,04). O número médio de dentes permanentes irrompidos foi maior no grupo sobrepeso / obesidade (p <0,001). A análise de regressão linear demonstrou que o estado nutricional, sexo e idade foram fortemente associados ao número de dentes permanentes irrompidos (p <0,05). Concluindo, nosso estudo demonstrou que o momento da erupção dos dentes permanentes é associado com sobrepeso / obesidade em crianças de Manaus, Brasil.

10.
Braz Dent J ; 29(2): 128-132, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29898057

RESUMO

Patients undergoing radiotherapy treatment present more susceptibility to dental caries and the use of an orthodontic device increases this risk factor due to biofilm accumulation around the brackets. The objective of this study was to evaluate the shear bond strength to irradiated permanent teeth of orthodontic brackets bonded with conventional glass ionomer cement and resin-modified glass ionomer cement due to the fluoride release capacity of these materials. Ninety prepared human premolars were divided into 6 groups (n=15), according to the bonding material and use or not of radiation: CR: Transbond XT composite resin; RMGIC: Fuji Ortho LC conventional glass ionomer cement; GIC: Ketac Cem Easymix resin-modified glass ionomer cement. The groups were irradiated (I) or non-irradiated (NI) prior to bracket bonding. The specimens were subjected to a fractioned radiation dose of 2 Gy over 5 consecutive days for 6 weeks. After the radiotherapy, the brackets were bonded on the specimens with Transbond XT, Fuji Ortho LC and Ketac Cem Easymix. After 24 h, the specimens were subjected to shear bond strength test. The image of enamel surface (classified by Adhesive Remnant Index - ARI) was also evaluated and its frequency was checked among groups/subgroups. The shear bond strength variable was evaluated with ANOVA and Tukey's post-hoc test. GIC group showed the lowest adhesion values among the groups (p<0.05). There was no statistically significant difference among non-irradiated and irradiated groups (p>0.05). As for the ARI, the CR-I group showed the highest material retention on enamel surface among the irradiated groups. RMGIC group showed the highest values for shear bond strength and presented ARI acceptable for clinical practices.


Assuntos
Dente Pré-Molar/efeitos da radiação , Colagem Dentária , Cimentos de Ionômeros de Vidro , Neoplasias/radioterapia , Braquetes Ortodônticos , Condicionamento Ácido do Dente , Resinas Compostas/química , Esmalte Dentário , Humanos , Técnicas In Vitro , Radiação Ionizante , Resistência ao Cisalhamento
11.
Rev. Cient. CRO-RJ (Online) ; 3(1): 8-15, Jan.-Apr. 2018.
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1021064

RESUMO

Objective: The objective of this study was to evaluate the tissue response and expression of matrix metalloproteinases (MMP) -2 and -9 to resinous and glass ionomer cements in direct contact with the subcutaneous connective tissue. Methods: RelyXTM Unicem resinous cement (RC; n=30), KetacTM Cem Easymix glass ionomer cement (GI; n=30), and polyethylene empty tubes (control; n=30) were implanted in the dorsal subcutaneous tissue of isogenic BALB/c mice, and the tissues were biopsied after 7, 21, and 63 days for histological analysis. The inflammatory cells and fibroblasts were counted, and the fibrous capsule thickness was measured. MMP -2 and MMP-9 expression levels were investigated by immunohistochemistry. Data were analyzed statistically (significance level=5%). Results: We found that RC induced a low inflammation at day 7 and 21, which was increased at day 63 (p<0.05). GI induced a more intense mononuclear inflammatory response at day 7 and 21 (p<0.05), which was reduced at day 63 to levels similar to the control (p>0.05). The fibrous capsule thickness was thin for RC, GI, and control (p>0.05).MMP-2 was detected early for GI and RC and decreased afterwards. MMP-9 presented a similar pattern for GI, whereas the MMP -9 expression was late for RC. Conclusion: Resinous cementRelyXTM Unicem induced an inflammatory response and late MMP -9 expression in the subcutaneous connective tissue that was different from that induced by KetacTM Cem Easymixglass ionomer cement.


Objetivo: Avaliar a resposta tecidual e a expressão de metaloproteinases da matriz (MMP) -2 e -9 frente a um cimento resinoso e um cimento ionomérico, após implantação no tecido conjuntivo subcutâneo de camundongos. Métodos: O cimento resinoso RelyXTM Unicem (CR; n=30), o cimento ionomérico KetacTM Cem Easymix (CI; n=30) e tubo de polietileno vazio (controle; n=30) foram implantados no tecido subcutâneo dorsal de camundongos isogênicos BALB/c e os tecidos removidos para análise histológica após 7, 21 e 63 dias. Foram analisadas a resposta celular local, por meio da contagem de células inflamatórias e a espessura da cápsula fibrosa. A expressão de MMP -2 e -9 foi investigada por meio de imunohistoquímica. Os dados foram submetidos à análise estatística (α=5%). Resultados: Foi observado que CR induziu uma inflamação leve aos 7 e 21 dias com aumento do número de células inflamatórias aos 63 dias (p<0,05). CI induziu uma resposta inflamatória mononuclear mais intensa aos 7 e 21 dias (p<0,05), com redução do infiltrado aos 63 dias, semelhante ao observado no controle (p>0,05). Em todos os grupos a espessura da cápsula foi considerada fina (p>0,05). MMP -2 foi detectada em períodos precoces para CR e CI, com diminuição com o passar do tempo. MMP -9 apresentou um padrão semelhante ao controle para o CI, enquanto para o CR houve aumento com o passar do tempo. Conclusão: O cimento resinoso RelyXTM Unicem induziu uma resposta inflamatória e a expressão de MMP -9 mais tardia no tecido conjuntivo subcutâneo que foi diferente da resposta induzida pelo cimento ionomérico KetacTM Cem Easymix.


Assuntos
Tecido Conjuntivo , Cimentos de Resina , Metaloproteinases da Matriz , Cimentos Dentários , Cimentos de Ionômeros de Vidro
12.
Braz. dent. j ; 29(2): 128-132, Mar.-Apr. 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-951540

RESUMO

Abstract Patients undergoing radiotherapy treatment present more susceptibility to dental caries and the use of an orthodontic device increases this risk factor due to biofilm accumulation around the brackets. The objective of this study was to evaluate the shear bond strength to irradiated permanent teeth of orthodontic brackets bonded with conventional glass ionomer cement and resin-modified glass ionomer cement due to the fluoride release capacity of these materials. Ninety prepared human premolars were divided into 6 groups (n=15), according to the bonding material and use or not of radiation: CR: Transbond XT composite resin; RMGIC: Fuji Ortho LC conventional glass ionomer cement; GIC: Ketac Cem Easymix resin-modified glass ionomer cement. The groups were irradiated (I) or non-irradiated (NI) prior to bracket bonding. The specimens were subjected to a fractioned radiation dose of 2 Gy over 5 consecutive days for 6 weeks. After the radiotherapy, the brackets were bonded on the specimens with Transbond XT, Fuji Ortho LC and Ketac Cem Easymix. After 24 h, the specimens were subjected to shear bond strength test. The image of enamel surface (classified by Adhesive Remnant Index - ARI) was also evaluated and its frequency was checked among groups/subgroups. The shear bond strength variable was evaluated with ANOVA and Tukey's post-hoc test. GIC group showed the lowest adhesion values among the groups (p<0.05). There was no statistically significant difference among non-irradiated and irradiated groups (p>0.05). As for the ARI, the CR-I group showed the highest material retention on enamel surface among the irradiated groups. RMGIC group showed the highest values for shear bond strength and presented ARI acceptable for clinical practices.


Resumo Pacientes submetidos a tratamento radioterápico apresentam maior suscetibilidade à cárie dentária e o uso de aparelho aumenta esse fator de risco devido ao acúmulo de biofilme dental ao redor dos bráquetes. Assim, o objetivo deste estudo foi avaliar a resistência ao cisalhamento de bráquetes ortodônticos colados com cimento de ionômero de vidro convencional (CIV) e o modificado por resina (CIVMR), devido à capacidade de liberação de flúor desse material em dentes permanentes irradiados. Noventa pré-molares humanos foram divididos em 6 grupos (n=15), de acordo com o materila de colagem e com o uso ou não de radiação: RC: Transbond XT; CIVMR: Fuji Ortho LC; RMGIC: Ketac Cem Easymix. Os grupos for irradiados (I) ou não-irradiados (NI) previamente à colagem dos bráquetes. Os espécimes foram submetidos a doses fracionadas de radiação de 2 Gy/dia, durante 5 dias consecutivos, por 6 semanas. Depois da termociclagem e radioterapia, os bráquetes foram colados sobre os espécimes com Transbond XT, Fuji Ortho LC e Ketac Cem Easymix. Depois de 24 h, os espécimes foram sumetidos ao teste de resistência ao cisalhamento. A imagem da superfície do esmalte (classificado pelo Índice de Remanescente Adesivo-IRA) também foi avaliada. O IRA foi distribuido em tabelas de contingência e analisado quanto à frequência entre grupos/subgrupos. O teste de resistência ao cisalhamento foi avaliado por ANOVA e pós-teste de Tukey. CIVMR apresentou os menores valores de adesão entre os grupos (p<0,05). Entre os grupos irradiados e não-irradiados não houve diferença estatística significante (p>0,05). Quanto ao IRA, RC-I apresentou maior retenção de compósito na superfície do esmalte do que os demais subgrupos. CIVMR pode ser utilizado para colagem de bráquetes metálico e apresentou IRA aceitável para práticas clínicas.

13.
Braz. oral res. (Online) ; 32: e121, 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-974461

RESUMO

Abstract The aim of this study was to evaluate the influence of radiation therapy on root canal sealer push-out bond strength (BS) to dentin and the sealer/dentin interface after different final irrigation solutions (NaOCl, EDTA, and chitosan). Sixty-four maxillary canines were distributed into two groups (n=30): non-irradiated and irradiated with 60 Gy. Canals were prepared with Reciproc-R50 and subdivided (n=10) for final irrigation (NaOCl, EDTA, chitosan) and filled. Three dentin slices were obtained from each root third. The first slice of each third was selected for BS evaluation, and the failure mode was determined by stereomicroscopy. SEM analysis of the sealer-dentin interface was performed in the remaining slices. Two-way ANOVA and Tukey's tests (α=0.05) were used. Lower BS (P<0.0001) was obtained after irradiation (2.07±0.79 MPa), regardless of the final irrigation solution used. The NaOCl group (P<0.001) had the lowest BS in the irradiated (1.68±0.72) and non-irradiated (2.39±0.89) groups, whereas the EDTA (irradiated: 2.14±0.77 and non-irradiated: 3.92±1.54) and chitosan (irradiated: 2.37±0.73 and non-irradiated: 3.51±1.47) groups demonstrated a higher BS (P<0.05). The highest values were observed in the coronal third (3.17±1.38) when compared to the middle (2.74±1.36) and apical ones (2.09±0.97)(P<0.0001). There were more cohesive failures and more gaps in irradiated specimens, regardless of the final solution. The present study showed that radiation was associated with a decrease in BS, regardless of the final solution used, whereas chitosan increased BS in teeth subjected to radiation therapy.

14.
J Appl Oral Sci ; 25(6): 612-619, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29211282

RESUMO

AIM: To compare the apical negative pressure irrigation (ANP) with conventional irrigation in the teeth of immature dogs with apical periodontitis. METHODS: Fifty-two immature pre-molar root canals were randomly assigned into 4 groups: ANP (n=15); conventional irrigation (n=17); healthy teeth (control) (n = 10); and teeth with untreated apical periodontitis (control) (n=10). After induction of apical periodontitis, teeth were instrumented using EndoVac® (apical negative pressure irrigation) or conventional irrigation. The animals were euthanized after 90 days. The sections were stained by HE and analyzed under conventional and fluorescence microscopy. TRAP histoenzymology was also performed. Statistical analyses were performed with the significance level set at 5%. RESULTS: There was difference in the histopathological parameters between ANP and conventional groups (p<0.05). The ANP group showed a predominance of low magnitude inflammatory infiltrate, a smaller periodontal ligament, and lower mineralized tissue resorption. There were no differences in the periapical lesion extensions between the ANP and conventional groups (p>0.05). However, a lower number of osteoclasts was observed in the ANP group (p<0.05). CONCLUSION: The EndoVac® irrigation system presented better biological results and more advanced repair process in immature teeth with apical periodontitis than the conventional irrigation system, confirming the hypothesis.


Assuntos
Periodontite Periapical/tratamento farmacológico , Irrigantes do Canal Radicular/farmacologia , Irrigação Terapêutica/métodos , Animais , Cães , Microscopia de Fluorescência , Periodontite Periapical/patologia , Distribuição Aleatória
15.
Braz. j. oral sci ; 16: e17065, jan.-dez. 2017. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-884055

RESUMO

Either obesity and underweight are public health concerns that affect the development of children. Aim: The aim of this study was to evaluate whether the nutritional status affects permanent tooth eruption chronology in Brazilian children. Methods: A hundred sixty children were examined by a pediatric dentistry and at the clinical examination, the number of erupted permanent teeth was evaluated. The anthropometric measurements of the children were determined, and they were divided into groups: underweight, eutrophic, overweight and obese. The association between delayed tooth eruption and nutritional status groups was evaluated using chi-square test. The Shapiro­Wilk test was used to verify the normality of the data. To compare the mean number of delayed teeth according to nutritional status' groups Kruskal-Wallis test with multiple comparison by Dunn's test was performed. Results: Fifty-six children had delayed tooth eruption in at least one permanent teeth and delayed tooth eruption was more common in underweight children than in eutrophic children (p=0.0091). Conclusion: In conclusion, our study suggests that underweight Brazilian children have a higher incidence of delayed eruption (AU)


Assuntos
Humanos , Masculino , Feminino , Criança , Criança , Odontologia , Estado Nutricional , Erupção Dentária
16.
J. appl. oral sci ; 25(6): 612-619, Nov.-Dec. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-893670

RESUMO

Abstract Aim: To compare the apical negative pressure irrigation (ANP) with conventional irrigation in the teeth of immature dogs with apical periodontitis. Methods: Fifty-two immature pre-molar root canals were randomly assigned into 4 groups: ANP (n=15); conventional irrigation (n=17); healthy teeth (control) (n = 10); and teeth with untreated apical periodontitis (control) (n=10). After induction of apical periodontitis, teeth were instrumented using EndoVac® (apical negative pressure irrigation) or conventional irrigation. The animals were euthanized after 90 days. The sections were stained by HE and analyzed under conventional and fluorescence microscopy. TRAP histoenzymology was also performed. Statistical analyses were performed with the significance level set at 5%. Results: There was difference in the histopathological parameters between ANP and conventional groups (p<0.05). The ANP group showed a predominance of low magnitude inflammatory infiltrate, a smaller periodontal ligament, and lower mineralized tissue resorption. There were no differences in the periapical lesion extensions between the ANP and conventional groups (p>0.05). However, a lower number of osteoclasts was observed in the ANP group (p<0.05). Conclusion: The EndoVac® irrigation system presented better biological results and more advanced repair process in immature teeth with apical periodontitis than the conventional irrigation system, confirming the hypothesis.


Assuntos
Animais , Cães , Periodontite Periapical/tratamento farmacológico , Irrigantes do Canal Radicular/farmacologia , Irrigação Terapêutica/métodos , Microscopia de Fluorescência , Periodontite Periapical/patologia , Distribuição Aleatória
17.
J Appl Oral Sci ; 24(4): 338-43, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27556204

RESUMO

OBJECTIVE: The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. MATERIAL AND METHODS: Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey's post hoc test (α=0.05). RESULTS: KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). CONCLUSIONS: KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.


Assuntos
Resinas Compostas/toxicidade , Metacrilatos/toxicidade , Resinas de Silorano/toxicidade , Fator de Necrose Tumoral alfa/análise , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Células Cultivadas , Resinas Compostas/efeitos da radiação , Luzes de Cura Dentária , Ensaio de Imunoadsorção Enzimática , L-Lactato Desidrogenase , Teste de Materiais , Metacrilatos/efeitos da radiação , Camundongos , Valores de Referência , Reprodutibilidade dos Testes , Resinas de Silorano/efeitos da radiação , Fatores de Tempo
18.
J. appl. oral sci ; 24(4): 338-343, July-Aug. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-792602

RESUMO

ABSTRACT The successful use of composite resins in Dentistry depends on physicochemical properties, but also on the biological compatibility of resins, because of the close association between pulp and dentin. Objective The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages. Material and Methods Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s post hoc test (α=0.05). Results KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05). Conclusions KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.


Assuntos
Animais , Camundongos , Fator de Necrose Tumoral alfa/análise , Resinas Compostas/toxicidade , Resinas de Silorano/toxicidade , Metacrilatos/toxicidade , Valores de Referência , Fatores de Tempo , Teste de Materiais , Ensaio de Imunoadsorção Enzimática , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Células Cultivadas , Reprodutibilidade dos Testes , Resinas Compostas/efeitos da radiação , Luzes de Cura Dentária , Resinas de Silorano/efeitos da radiação , L-Lactato Desidrogenase , Metacrilatos/efeitos da radiação
19.
J Appl Oral Sci ; 23(5): 523-8, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26537723

RESUMO

OBJECTIVES: To evaluate the microbial contamination of pacifiers by Mutans Streptococci(MS) and the efficacy of different methods for their disinfection. METHODS: Twenty-eight children were assigned to a 4-stage changeover system with a 1-week interval. In each stage, children received a new pacifier and the parents were instructed to maintain their normal habits for 1 week. After this time, the pacifiers were subjected to the following 4 disinfection methods: spraying with 0.12% chlorhexidine solution, Brushtox or sterile tap water, and immersion in boiling tap water for 15 minutes. Microbiological culture for MS and Scanning Electron Microscopy (SEM) were performed. The results were analyzed statistically by Friedman's non-parametric test (a=0.05). RESULTS: The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox spray and control (p<0.05). The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture. CONCLUSIONS: Pacifiers become contaminated by MS after their use by children and should be disinfected routinely. Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox).


Assuntos
Clorexidina/farmacologia , Desinfecção/métodos , Antissépticos Bucais/farmacologia , Chupetas/microbiologia , Streptococcus mutans/efeitos dos fármacos , Água/química , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Pré-Escolar , Contagem de Colônia Microbiana , Desinfetantes/farmacologia , Temperatura Alta , Humanos , Imersão , Lactente , Microscopia Eletrônica de Varredura , Estatísticas não Paramétricas , Streptococcus mutans/crescimento & desenvolvimento , Fatores de Tempo
20.
J. appl. oral sci ; 23(5): 523-528, Sept.-Oct. 2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-764154

RESUMO

Objectives To evaluate the microbial contamination of pacifiers by Mutans Streptococci(MS) and the efficacy of different methods for their disinfection.Methods Twenty-eight children were assigned to a 4-stage changeover system with a 1-week interval. In each stage, children received a new pacifier and the parents were instructed to maintain their normal habits for 1 week. After this time, the pacifiers were subjected to the following 4 disinfection methods: spraying with 0.12% chlorhexidine solution, Brushtox® or sterile tap water, and immersion in boiling tap water for 15 minutes. Microbiological culture for MS and Scanning Electron Microscopy (SEM) were performed. The results were analyzed statistically by Friedman’s non-parametric test (a=0.05).Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox®spray and control (p<0.05). The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture.Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely. Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).


Assuntos
Pré-Escolar , Humanos , Lactente , Clorexidina/farmacologia , Desinfecção/métodos , Antissépticos Bucais/farmacologia , Chupetas/microbiologia , Streptococcus mutans/efeitos dos fármacos , Água/química , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Contagem de Colônia Microbiana , Desinfetantes/farmacologia , Temperatura Alta , Imersão , Microscopia Eletrônica de Varredura , Estatísticas não Paramétricas , Streptococcus mutans/crescimento & desenvolvimento , Fatores de Tempo
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