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1.
J Contemp Dent Pract ; 22(3): 268-272, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34210927

RESUMO

AIM: To evaluate the active tactile sensitivity in individuals with complete natural dentition, determining the smallest thickness detected by the participants, and clarifying if there is a difference between the thicknesses analyzed. MATERIALS AND METHODS: Active tactile sensitivity was evaluated in 40 research participants. Inclusion criteria included participants with complete natural dentition, without active or history of periodontal disease, absence of temporomandibular disorders, bruxism, and restorations in the evaluated area. Exclusion criteria included age below 18 years. The active tactile perception threshold was evaluated by using carbon sheets of different thicknesses (0, 12, 24, 40, 80, 100, and 200 µm), which were inserted in the participants' premolars, bilaterally. The carbon sheet was inserted so as not to come into contact with the oral soft tissues. Subsequently, the participant occluded and was asked about the perception of the intraocclusal object 20 times in each occlusal contact. The collected data were tabulated considering the amount of positive and negative responses for each carbon thickness. Values of p < 0.05 were considered significant. RESULTS: The results showed that there was linearity in perception, on both sides, besides, the natural dentition was able to perceive difference in thickness from 12 µm. CONCLUSION: We conclude that the 12 µm thickness is noticeable in occlusion and can be differentiated from other thicknesses in natural dentition and that there is no difference between the tactile sensitivity of the right and left sides. CLINICAL SIGNIFICANCE: A better understanding of active oral tactile sensitivity will contribute to numerous clinical applications in dentistry, including occlusal adjustment in dental rehabilitation, dental implants prosthesis design, and survival of prosthetic rehabilitation.


Assuntos
Bruxismo , Boca Edêntula , Adolescente , Oclusão Dentária , Dentição , Humanos , Tato
2.
Cranio ; : 1-11, 2019 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-31264537

RESUMO

Objective: To evaluate the association between polymorphisms in genes and comorbid presence of arthralgias and TMD. Methods: This is a case-control study. The groups formed were individuals with chronic arthralgia and 1) myofascial pain (n = 42); 2) articular (n = 16); 3) multiple diagnoses (n = 69); 4) with TMD and without some other arthralgia (n = 16); 5) without TMD but with pain in other joints (n = 82); and 6) a control group (n = 72). SNPs in COMT, ADRB2, and HTR1A genes were investigated. Results: The CT genotype for the COMT (rs9332377) gene was associated with the absence of myofascial pain (p = .05). In the ADRB2 (rs1042713) gene, the AA genotype was associated with the absence of myofascial pain (p = .03). Discussion: This study supports the hypothesis that alterations in the COMT, ADRB2, and HTR1A genes influence the presence of chronic pain and TMD.

3.
Mol Genet Genomic Med ; 6(5): 689-701, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30078197

RESUMO

Dentistry constitutes the basic nucleus of professionals of higher level of health in Brazil with one of the largest concentrations of dentists per capita in the world. However, the genetic in dentistry in Brazil is explored, basically, in research field. Future actions need to be performed in order to deep the whole knowledge about diagnosis and treatment of diseases with genetic basis in dentistry, in Brazil.


Assuntos
Genética Médica , Doenças Dentárias/genética , Brasil , Humanos
4.
Braz. dent. j ; 29(1): 14-22, Jan.-Feb. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-888726

RESUMO

Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.


Resumo A história de periodontite crônica (CP) é um fator de risco para falhas na osseointegração. O sistema de osteoclastogênese (RANK, RANKL e OPG) é crucial para o controle da homeostase óssea. O objetivo deste estudo foi investigar os níveis de OPG e RANKL no tecido peri-implantar de voluntários com e sem histórico de CP e sua associação com inflamação da mucosa. Este é um estudo tipo caso-controle. O exame para diagnóstico de CP e na região peri-implantar foi realizado em 46 voluntários, divididos em controle (sem história CP, n=26) e grupo CP (com histórico de CP, n=20). Descartes gengivais foram obtidos durante a exposição do implante. PCR quantitativo avaliou a expressão do RNAm de OPG/RANKL. As proteínas OPG e RANKL foram analisadas por western blot e imunohistoquímica. O teste do qui-quadrado analisou a significância entre as variáveis nominais enquanto as variáveis contínuas foram analisadas pelo teste-t e Mann-Whitney, após o teste de Shapiro-wilk. O método do Livak 2--ΔΔCT avaliou a expressão gênica. Os voluntários com CP apresentaram 23 vezes mais chances de desenvolver inflamação da mucosa. Expressão elevada no RNAm de RANKL (p=0.04) e RANKL/OPG (p=0.001) foram observadas no grupo CP. Voluntários com CP mostraram aumento dos níveis da proteína RANKL em contraste com diminuída expressão de OPG. Mesmo sem periodontite ativa, voluntários com histórico de CP apresentaram elevado nível gengival de RANKL/OPG e alta correlação com inflamação peri-implantar e perda do implante.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Periodontite Crônica/metabolismo , Implantes Dentários , Mucosa Bucal/patologia , Osteoprotegerina/metabolismo , Ligante RANK/metabolismo , Western Blotting , Periodontite Crônica/patologia , Imuno-Histoquímica , Osteoprotegerina/genética , Reação em Cadeia da Polimerase , Ligante RANK/genética , RNA Mensageiro/genética
5.
Braz Dent J ; 29(1): 14-22, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29267518

RESUMO

tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.


Assuntos
Periodontite Crônica/metabolismo , Implantes Dentários , Mucosa Bucal/patologia , Osteoprotegerina/metabolismo , Ligante RANK/metabolismo , Idoso , Western Blotting , Periodontite Crônica/patologia , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Osteoprotegerina/genética , Reação em Cadeia da Polimerase , Ligante RANK/genética , RNA Mensageiro/genética
6.
J Oral Maxillofac Surg ; 76(2): 314.e1-314.e9, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29175417

RESUMO

PURPOSE: The high prevalence of painful temporomandibular disorders (TMDs) in women suggests that estrogen and its receptors play a fundamental etiologic role in the development of this joint pathology through complex action mechanisms. The aim of this study was to evaluate the possible association between polymorphisms in the ESR1 (estrogen receptor-1) and ESRRB (estrogen-related receptor-ß) genes and the risk of simultaneous development of TMDs and pain in other joints in the body. MATERIALS AND METHODS: All participants were clinically evaluated for the presence of TMD (Research Diagnostic Criteria for TMD) and asked about the presence of chronic joint pain. The control group consisted of 72 patients without TMD and without pain. Participants with arthralgia were divided into 3 groups: with muscular TMD (n = 42), with articular TMD (n = 16), and without TMD and with systemic arthralgia (n = 82). Eight single-nucleotide polymorphisms in the ESR1 (rs12154178, rs1884051, rs2273206, rs7774230) and ESRRB (rs1676303, rs4903399, rs10132091, rs7151924) genes were investigated. The χ2 test and Student t and Mann-Whitney tests were used to assess the relevance of nominal and continuous variables, respectively. A P value less than .05 was considered significant. RESULTS: The TT (timin/timin) genotype for the ESR1 (rs2273206) gene was strongly associated with the risk of developing muscle TMDs and temporomandibular joint pain (P = .04). For the ESRRB (rs1676303) gene, an association was observed between the CC (cytosine/cytosine) genotype and the presence of articular TMDs associated with other chronic arthralgia (P = .02). These results were confirmed by the increased risk of developing articular TMDs associated with the C allele (P = .04). CONCLUSIONS: This study supports the hypothesis that changes in the ESR1 and ESRRB genes influence the presence of TMDs associated with chronic joint pain.


Assuntos
Artralgia/genética , Receptor alfa de Estrogênio/genética , Polimorfismo de Nucleotídeo Único , Receptores de Estrogênio/genética , Transtornos da Articulação Temporomandibular/genética , Adulto , Alelos , Estudos Transversais , Feminino , Genótipo , Haplótipos , Humanos , Masculino
7.
Braz Dent J ; 27(4): 367-74, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27652695

RESUMO

Despite the success of osseointegrated implants, failures have increased significantly, associated with development of peri-implantitis. Multiple factors influence the peri-implant bone loss, including environmental and genetic causes. BMPs (Bone morphogenetic proteins) are growth factors that induce bone formation. FGF (fibroblast growth factors) and their receptors (FGFRs) play important roles by controlling the levels of cell proliferation, differentiation and migration. BMP/FGF relationship is responsible for promoting bone regeneration and bone loss. The aim of this study was to analyze the correlation between BMP4, FGF3, FGF10 and FGFR1 genes and peri-implant bone loss. Two hundred and fifteen volunteers, with 754 dental implants, were submitted to oral examination and divided in healthy group (n=129) and peri-implantitis group (n=86). Thirteen polymorphisms in BMP4, FGF3, FGF10 and FGFR1 genes were analyzed individually and in haplotype. The chi-square test correlated genotypes, allelic and haplotype frequencies. Values of p<0.05 were considered significant. Volunteers with peri-implantitis demonstrated high incidence of total edentulism (p<0.0001) and thin peri-implant phenotype (p<0.04). Higher incidence of spontaneous bleeding, plaque and implant mobility was observed in peri-implantitis group (p<0.0001 for all). The TT polymorphic genotype for BMP4 rs2761884 was associated with healthy peri-implant (p=0.01). FGF3 rs4631909 (TT+CT genotype) also showed association with the control group (p=0.04). The frequency of C allele for FGF3 rs4631909 showed a tendency for association with peri-implantitis (p=0.08). FGF10 CCTG (p=0.03), BMP4 GAAA (p=0.05) and GGGA (p=0.02) haplotypes were associated with peri-implantitis (p=0.03). Therefore, it may be concluded that BMP4 and FGF10 haplotypes are associated with peri-implantitis.


Assuntos
Proteína Morfogenética Óssea 4/genética , Fatores de Crescimento de Fibroblastos/genética , Predisposição Genética para Doença , Haplótipos , Peri-Implantite/genética , Adulto , Estudos Transversais , Método Duplo-Cego , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade
8.
Braz. dent. j ; 27(4): 367-374, July-Aug. 2016. tab
Artigo em Inglês | LILACS | ID: lil-794608

RESUMO

Abstract Despite the success of osseointegrated implants, failures have increased significantly, associated with development of peri-implantitis. Multiple factors influence the peri-implant bone loss, including environmental and genetic causes. BMPs (Bone morphogenetic proteins) are growth factors that induce bone formation. FGF (fibroblast growth factors) and their receptors (FGFRs) play important roles by controlling the levels of cell proliferation, differentiation and migration. BMP/FGF relationship is responsible for promoting bone regeneration and bone loss. The aim of this study was to analyze the correlation between BMP4, FGF3, FGF10 and FGFR1 genes and peri-implant bone loss. Two hundred and fifteen volunteers, with 754 dental implants, were submitted to oral examination and divided in healthy group (n=129) and peri-implantitis group (n=86). Thirteen polymorphisms in BMP4, FGF3, FGF10 and FGFR1 genes were analyzed individually and in haplotype. The chi-square test correlated genotypes, allelic and haplotype frequencies. Values of p<0.05 were considered significant. Volunteers with peri-implantitis demonstrated high incidence of total edentulism (p<0.0001) and thin peri-implant phenotype (p<0.04). Higher incidence of spontaneous bleeding, plaque and implant mobility was observed in peri-implantitis group (p<0.0001 for all). The TT polymorphic genotype for BMP4 rs2761884 was associated with healthy peri-implant (p=0.01). FGF3 rs4631909 (TT+CT genotype) also showed association with the control group (p=0.04). The frequency of C allele for FGF3 rs4631909 showed a tendency for association with peri-implantitis (p=0.08). FGF10 CCTG (p=0.03), BMP4 GAAA (p=0.05) and GGGA (p=0.02) haplotypes were associated with peri-implantitis (p=0.03). Therefore, it may be concluded that BMP4 and FGF10 haplotypes are associated with peri-implantitis.


Resumo Apesar do alto índice de sucesso em implantodontia, falhas tem aumentado drasticamente, estando associadas ao desenvolvimento de peri-implantite. A perda óssea peri-implantar é influenciada por múltiplos fatores, incluindo causas genéticas e ambientais. As BMPs (proteínas ósseas morfogenéticas) são fatores de crescimento indutores da formação óssea. Os FGFs (fatores de crescimento dos fibroblastos) e seus receptores (FGFRs) desenvolvem importante função na proliferação, diferenciação e migração celular. A relação BMP/FGF é responsável pela regeneração e perda óssea. O objetivo deste estudo foi estudar a possível correlação entre os genes BMP4, FGF3, FGF10 e FGFR1 e a perda óssea peri-implantar. Duzentos e quinze voluntários, com 754 implantes, foram submetidos ao exame oral e divididos em grupo saúde (n=129) e peri-implantite (n=86). Treze polimorfismos nos genes BMP4, FGF3, FGF10 e FGFR1 foram analisados individualmente e como haplótipos. O teste do qui-quadrado correlacionou as frequências dos genótipos, alelos e haplótipos. Valores de p<0,05 foram considerados estatisticamente significantes. Voluntários com peri-implantite mostraram alta incidência de edentulismo total (p<0,0001) e biotipo periodontal fino (p<0,04). Sangramento espontâneo, placa e mobilidade do implante foram altamente incidentes no grupo peri-implantite (p<0,0001). O genótipo polimórfico TT para BMP4 rs2761884 foi associado com saúde peri-implantar (p=0,01). FGF3 rs4631909 (genótipos TT+CT) mostraram associação com o grupo controle (p=0,04). A frequência do alelo C para FGF3 rs4631909 mostrou uma tendência de associação com peri-implantite (p=0,08). Os haplótipos FGF10 CCTG (p=0,03), BMP4 GAAA (p=0,05) e GGGA (p=0,02) foram associados com peri-implantite (p=0,03). Sendo assim, conclui-se que os haplótipos BMP4 e FGF10 estão associados com peri-implantite.


Assuntos
Humanos , Masculino , Feminino , Lactente , Adulto , Pessoa de Meia-Idade , Proteína Morfogenética Óssea 4/genética , Estudos Transversais , Fatores de Crescimento de Fibroblastos/genética , Predisposição Genética para Doença , Haplótipos , Peri-Implantite/genética , Método Duplo-Cego
9.
Braz Dent J ; 27(2): 128-34, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27058373

RESUMO

Subjects susceptible to chronic periodontitis (CP) show a high risk for the development of periimplantitis (PI). Both diseases are multifactorial, presenting similarities in their pathophysiology and polygenic profile. MMP-13 (matrix metalloproteinases 13/ collagenase 3) is a collagenolytic enzyme, which expression is induced by TGF beta 3 (transforming growth factor type 3) in human gingival fibroblasts and inhibited by TIMP-2 (tissue inhibitor of metalloproteinase type 2). The aim of this study was to investigate the occurrence of periimplantitis (PI) in subjects with history of chronic periodontitis (CP) and polymorphisms frequency in MMP13, TIMP2 and TGFB3 genes. One hundred and sixty-three volunteers received dental implant placement were submitted to oral and radiographic examination in order to identify past history of CP or presence of PI. Volunteers were divided into 4 groups: Control (without PI and CP, n=72), CP (with CP and without PI, n=28), PI (with PI and without CP, n=28) and diseased (with CP and PI, n=35). The chi-square test correlated genotypes in specific regions of MMP13 (rs2252070), TIMP2 (rs7501477) and TGFB3 (rs2268626) genes, considering the interaction between CP and PI. The results showed that volunteers with CP had 3.2 times more susceptibility to develop PI (p=0.0004) compared to those without CP. No significant association was observed in MMP13, TIMP2 and TGFB3 genes with CP or PI. CP is a risk factor to develop PI, however, there is no association of both diseases with polymorphisms in the MMP13, TIMP2 and TGFB3 genes.


Assuntos
Peri-Implantite/genética , Periodontite/genética , Polimorfismo de Nucleotídeo Único , Idoso , Brasil , Estudos de Casos e Controles , Doença Crônica , Feminino , Humanos , Masculino , Metaloproteinase 13 da Matriz , Pessoa de Meia-Idade , Inibidor Tecidual de Metaloproteinase-2 , Fator de Crescimento Transformador beta3
10.
J Contemp Dent Pract ; 17(1): 58-62, 2016 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-27084864

RESUMO

BACKGROUND: Alternative pretreatment strategies of dentin and adhesionare constantly being developed and studied with the goal of improving the adhesion of resin restorative materials with this tissue. The objectives of the present study were to evaluate the ability of airborne-particle abrasion (APA) with aluminum oxide on dentin to remove the smear layer and the effects produced on the dentin microstructure. MATERIALS AND METHODS: The phosphoric acid (PA) was used for a comparison. For that, 20 human third molars were randomly allocated into two experimental groups, according to the dentin pretreatment method used: G1 (N = 10) - PA, G2 (N = 10) -APA. For dentin surface analyses, an environmental scanning electron microscope (ESEM) was employed to observe dentin surfaces before and after the procedures. Before pretreatment, the specimens of both groups were smear covered. RESULTS: After pretreatment, the G1 images revealed dentin tubule orifices opened, enlarged and some erosive effects. (G2) exposed tubule orifices without enlargement, but crack-like alterations were observed on the surfaces. In this way, APA with aluminum oxide was able to remove the smear layer. CONCLUSION: The influences of the dentin roughness on adhesion and the consequences on dentin integrity and hardness need further investigations. CLINICAL SIGNIFICANCE: A good conditioning of the dentin before cementation is necessary in order to obtain a satisfactory rehabilitation in adhesive dentistry. So, it is necessary to know all methods to do it.


Assuntos
Condicionamento Ácido do Dente , Colagem Dentária , Dentina , Cimentos de Resina , Materiais Dentários , Adesivos Dentinários , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Camada de Esfregaço , Propriedades de Superfície
11.
Braz. dent. j ; 27(2): 128-134, Mar.-Apr. 2016. tab
Artigo em Inglês | LILACS | ID: lil-778338

RESUMO

Abstract Subjects susceptible to chronic periodontitis (CP) show a high risk for the development of peiimplantitis (PI). Both diseases are multifactorial, presenting similarities in their pathophysiology and polygenic profile. MMP-13 (matrix metalloproteinases 13/ collagenase 3) is a collagenolytic enzyme, which expression is induced by TGF beta 3 (transforming growth factor type 3) in human gingival fibroblasts and inhibited by TIMP-2 (tissue inhibitor of metalloproteinase type 2). The aim of this study was to investigate the occurrence of peiimplantitis (PI) in subjects with history of chronic periodontitis (CP) and polymorphisms frequency in MMP13, TIMP2 and TGFB3 genes. One hundred and sixty-three volunteers received dental implant placement were submitted to oral and radiographic examination in order to identify past history of CP or presence of PI. Volunteers were divided into 4 groups: Control (without PI and CP, n=72), CP (with CP and without PI, n=28), PI (with PI and without CP, n=28) and diseased (with CP and PI, n=35). The chi-square test correlated genotypes in specific regions of MMP13 (rs2252070), TIMP2 (rs7501477) and TGFB3 (rs2268626) genes, considering the interaction between CP and PI. The results showed that volunteers with CP had 3.2 times more susceptibility to develop PI (p=0.0004) compared to those without CP. No significant association was observed in MMP13, TIMP2 and TGFB3 genes with CP or PI. CP is a risk factor to develop PI, however, there is no association of both diseases with polymorphisms in the MMP13, TIMP2 and TGFB3 genes.


Resumo Indivíduos susceptíveis à periodontite crônica (CP) apresentam alto risco para o desenvolvimento de periimplantite (PI). Ambas doenças são multifatoriais e apresentam similaridades na patofisiologia e perfil poligênico. A MMP-13 (metaloproteinase da matriz tipo 13) é uma enzima colagenolítica cuja expressão é induzida por TGF beta 3 (fator transformador do crescimento tipo 3) nos fibroblastos gengivais humanos e inibida por TIMP-2 (inibidor tecidual de metaloproteinase tipo 2). O objetivo deste estudo foi investigar a ocorrência de periimplantite em sujeitos com periodontite crônica e a frequência dos polimorfismos nos genes MMP13, TIMP2 e TGFB3. Cento e sessenta e três voluntários submetidos à instalação de implantes endósseos foram analisados clínica e radiograficamente quanto à presença de histórico de CP e PI, sendo divididos em 4 grupos: Controle (sem história de CP e PI, n=72), CP (com CP e sem PI, n=28), PI (com PI e sem CP, n=28) e Doentes (com CP e PI, n=35). O teste do qui-quadrado correlacionou os genótipos nas regiões dos genes MMP13 (rs2252070), TIMP2 (rs7501477) e TGFB3 (rs2268626), considerando a interação entre CP e PI. Os resultados mostraram que voluntários com CP possuem 3.2 vezes mais chances de desenvolver PI (p=0.0004) comparados aos sem CP. Nenhuma associação significativa foi observada entre os genes MMP13, TIMP2 e TGFB3 e CP ou PI. A CP é um fator de risco ao desenvolvimento de PI, no entanto, não há associação entre ambas as doenças com polimorfismos nos genes MMP13, TIMP2 e TGFB3.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Peri-Implantite/genética , Periodontite/genética , Polimorfismo de Nucleotídeo Único , Brasil , Estudos de Casos e Controles , Doença Crônica , Metaloproteinase 13 da Matriz , Inibidor Tecidual de Metaloproteinase-2 , Fator de Crescimento Transformador beta3
12.
J Sci Med Sport ; 18(2): 150-5, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24661680

RESUMO

OBJECTIVES: To investigate whether genetic variants can be correlated with tendinopathy in elite male volleyball athletes. DESIGN: Case-control study. METHODS: Fifteen single nucleotide polymorphisms within BMP4, FGF3, FGF10, FGFR1 genes were investigated in 138 elite volleyball athletes, aged between 18 and 35 years, who undergo 4-5h of training per day: 52 with tendinopathy and 86 with no history of pain suggestive of tendinopathy in patellar, Achilles, shoulder, and hip abductors tendons. The clinical diagnostic criterion was progressive pain during training, confirmed by magnetic resonance image. Genomic DNA was obtained from saliva samples. Genetic markers were genotyped using TaqMan real-time PCR. Chi-square test compared genotypes and haplotype differences between groups. Multivariate logistic regression analyzed the significance of covariates and incidence of tendinopathy. RESULTS: Statistical analysis revealed participant age (p=0.005) and years of practice (p=0.004) were risk factors for tendinopathy. A significant association between BMP4 rs2761884 (p=0.03) and tendinopathy was observed. Athletes with a polymorphic genotype have 2.4 times more susceptibility to tendinopathy (OR=2.39; 95%CI=1.10-5.19). Also, association between disease and haplotype TTGGA in BMP4 (p=0.01) was observed. The FGF3 TGGTA haplotype showed a tendency of association with tendinopathy (p=0.05), and so did FGF10 rs900379. FGFR1 showed no association with disease. CONCLUSIONS: These findings indicate that haplotypes in BMP4 and FGF3 genes may contribute to the tendon disease process in elite volleyball athletes.


Assuntos
Proteína Morfogenética Óssea 4/genética , Fator 3 de Crescimento de Fibroblastos/genética , Tendinopatia/genética , Voleibol/lesões , Adulto , Estudos de Casos e Controles , Predisposição Genética para Doença , Haplótipos , Humanos , Masculino , Adulto Jovem
13.
Periodontia ; 25(2): 7-12, 2015. ilus, tab, graf
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-772741

RESUMO

Diversos estudos têm demonstrado que indivíduos com susceptibilidade genética à doença peri-implantar podem apresentar maior risco de perda do implante dentário. Objetivo: O objetivo deste estudo foi correlacionar os aspectos clínicos e radiográficos no tecido peri-implantar, apresentando condições saudáveis e doentes, com o polimorfismo genético da interleucina-10 (IL-10). Material e métodos: 109 pacientes foram divididos em três grupos. O grupo controle foi caracterizado pela saúde peri-implantar (n=51). Os grupos teste foram divididos em mucosite (n=21; indivíduos com inflamação da mucosa peri-implantar) e peri-implantite (n = 37; perda óssea patológica peri-implantar). Os tecidos peri-implantares foram examinados clinicamente considerando o sangramento à sondagem, a cor da mucosa e a mobilidade do implante. Amostras de saliva foram coletadas para análise do polimorfismo genético da IL-10, na região promotora 592, utilizando a enzima de restrição Rsal. Resultados: Clinicamente, as regiões do grupo mucosite foram caracterizadas pela cor vermelha da mucosa e presença de sangramento à sondagem em todos os pacientes. O grupo peri-implantite mostrou mucosa avermelhada em 15 pacientes (40,5%), mas sangramento à sondagem em 9 pacientes (24,3%). Mobilidade do implante foi observada em quatro regiões (10,8%). A média de perda óssea patológica radiográfica foi de 3,8 ± 1,5 exposições de roscas. Análise do polimorfismo genético para IL-10-592, por meio do teste qui-quadrado, não apresentou diferença significativa entre os grupos saudável e testes. Conclusões: Polimorfismo na IL10-592 não está associado com o desenvolvimento da doença peri-implantar. A presença de sangramento à sondagem não caracteriza a doença peri-implantar.


Several studies have shown that individuals with genetic susceptibility to peri-implant disease may be at greater risk of loss of the dental implant. Objective: The objective of this study was to correlate the clinical and radiographic findings in the peri-implant tissue, showing healthy conditions and patients with the genetic polymorphism of interleukin-10 (IL-10). Material and methods: 109 patients were divided into three groups. The control group was characterized by periimplant health (n = 51). The test groups were divided into mucositis (n = 21; patients with inflammation of the periimplant mucosa) and peri-implant (n = 37; pathological bone loss peri-implant). The peri-implant tissues were examined clinically considering bleeding on probing, the color of the mucosa and the mobility of the implant. Saliva samples were collected for analysis of genetic polymorphism of IL-10 in the promoter region 592, using the restriction enzyme RsaI. Results: Clinically, mucositis group regions were characterized by the color red and the presence of mucosal bleeding on probing in all patients. The peri-implant group showed reddish mucosa in 15 patients (40.5%), but bleeding on probing in 9 patients (24.3%). Mobility of the implant was observed in four regions (10.8%). The mean radiographic pathological bone loss was 3.8 ± 1.5 exhibition threads. Analysis of genetic polymorphism for IL-10-592, using the chi-square test showed no significant difference between the healthy group and testing. Conclusions: Polymorphism in IL10-592 is not associated with the development of peri-implant disease. The presence of bleeding on probing does not characterize the peri-implant disease.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Peri-Implantite , Polimorfismo Genético
14.
BMC Oral Health ; 14: 84, 2014 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-25008200

RESUMO

BACKGROUND: There is evidence for a genetic contribution to chronic periodontitis. In this study, we conducted a genome wide association study among 866 participants of the University of Pittsburgh Dental Registry and DNA Repository, whose periodontal diagnosis ranged from healthy (N = 767) to severe chronic periodontitis (N = 99). METHODS: Genotypingi of over half-million single nucleotide polymorphisms was determined. Analyses were done twice, first in the complete dataset of all ethnicities, and second including only samples defined as self-reported Whites. From the top 100 results, twenty single nucleotide polymorphisms had consistent results in both analyses (borderline p-values ranging from 1E-05 to 1E-6) and were selected to be tested in two independent datasets derived from 1,460 individuals from Porto Alegre, and 359 from Rio de Janeiro, Brazil. Meta-analyses of the Single nucleotide polymorphisms showing a trend for association in the independent dataset were performed. RESULTS: The rs1477403 marker located on 16q22.3 showed suggestive association in the discovery phase and in the Porto Alegre dataset (p = 0.05). The meta-analysis suggested the less common allele decreases the risk of chronic periodontitis. CONCLUSIONS: Our data offer a clear hypothesis to be independently tested regarding the contribution of the 16q22.3 locus to chronic periodontitis.


Assuntos
Mapeamento Cromossômico , Cromossomos Humanos Par 16/genética , Periodontite Crônica/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Cromossomos Humanos Par 21/genética , Periodontite Crônica/etnologia , Complicações do Diabetes , Grupos Étnicos/genética , Grupo com Ancestrais do Continente Europeu/genética , Feminino , Frequência do Gene/genética , Marcadores Genéticos/genética , Predisposição Genética para Doença/genética , Variação Genética/genética , Estudo de Associação Genômica Ampla , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco , Fumar
15.
Rev. bras. odontol ; 68(2): 225-228, jul.-dez. 2011.
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-857511

RESUMO

As desordens musculares geralmente estão presentes na Disfunção Temporomandibular (DTM). Sua principal sintomatologia é dor, edema, proveniente de injúria tecidual e inflamação. O tratamento das DTMs consiste em tratamento suporte para alívio da sintomatologia e “definitivo” para eliminar as causas e fatores que são perpetuantes. O uso de antiinflamatórios (AINEs), relaxantes musculares, placas oclusais, fitoterápicos com ação antiinflamatória como Arnica Montana são indicados como tratamento suporte. A Arnica montana tem sido usada para redução de inflamação e dor causada por entorse, contusões e ferimentos. Sua principal ação é inibição da ativaçãodo fator de transcrição celular NF- қB.


Assuntos
Arnica/uso terapêutico , Doenças Musculares/terapia , Dor Facial/terapia , Inflamação/terapia , Síndrome da Disfunção da Articulação Temporomandibular/terapia
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