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1.
J Vet Diagn Invest ; 35(5): 535-542, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37377189

RESUMO

Although bovine respiratory syncytial virus (BRSV) infection has been reported in cattle in Argentina, it has not been associated with pneumonia in Argentina. We report here 5 cases of bovine pneumonia associated with BRSV. Autopsies were performed on 35 beef cattle with gross and/or microscopic lesions of pneumonia from 3 commercial feedlots. Lung samples in 5 of 35 animals were BRSV-positive by reverse-transcription nested PCR. The lungs of 2 of these 5 animals were coinfected with Mannheimia haemolytica, and 1 with bovine viral diarrhea virus 1. Microscopically, the lungs of 3 of the 5 BRSV PCR-positive animals had fibrinosuppurative bronchopneumonia, with or without pleuritis; 2 of the 5 had interstitial pneumonia. We conclude that BRSV is part of the bovine respiratory disease complex in Argentina.


Assuntos
Complexo Respiratório Bovino , Doenças dos Bovinos , Infecções por Vírus Respiratório Sincicial , Vírus Sincicial Respiratório Bovino , Bovinos , Animais , Infecções por Vírus Respiratório Sincicial/epidemiologia , Infecções por Vírus Respiratório Sincicial/veterinária , Infecções por Vírus Respiratório Sincicial/patologia , Argentina/epidemiologia , Doenças dos Bovinos/patologia , Pulmão/patologia
2.
Arch Cardiol Mex ; 91(4): 470-479, 2021 Nov 01.
Artigo em Espanhol | MEDLINE | ID: mdl-34491249

RESUMO

Atrial isomerism describes complex anatomical findings with defects in the determination of lateralization; being a rare situation, with a prevalence of 1 in every 10.000 to 20.000 live births, with an incidence of up to 4% of all cardiac malformations. The diagnosis can be made in the neonatal age; however, clinical presentation is nonspecific. Depending on the spectrum of malformations, complex and invasive diagnostic tools may be required. Treatment is varied and can range from palliative surgery in view of univentricular physiology to total correction surgery for biventricular repair.


El isomorfismo cardiaco describe hallazgos anatómicos complejos con defectos en la determinación de la lateralización. Es una situación poco frecuente, con prevalencia de 1 en cada 10,000 a 20,000 nacidos vivos, con incidencia hasta del 4% de todas las malformaciones cardiacas. El diagnóstico puede realizarse en la etapa neonatal; sin embargo, el cuadro clínico es inespecífico. De acuerdo con el espectro de malformaciones se pueden necesitar medios diagnósticos complejos e invasivos. El tratamiento es variado y puede ir desde la cirugía paliativa en vista de una fisiología univentricular hasta una cirugía de corrección total para una reparación biventricular.


Assuntos
Cardiopatias Congênitas , Síndrome de Heterotaxia , Cardiopatias Congênitas/cirurgia , Humanos
3.
Vet Pathol ; 54(4): 620-628, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28622490

RESUMO

The purpose of this study was to follow the progression of gross and histologic lesions and apoptosis events in Lawsonia intracellularis-infected enterocytes through the course of the disease, proliferative enteropathy (PE). Thirty 5-week-old pigs were divided into 2 groups: 20 challenged and 10 control animals. Groups of 3 pigs, 2 challenged and 1 control, were euthanized at 1, 3, 5, 8, 11, 15, 19, 24, 29, and 35 days after inoculation. Complete necropsies were performed with gross evaluation. Tissue samples from different sites of the gastrointestinal tract and other visceral organs were collected for routine histologic staining and for immunohistochemistry (IHC) for L. intracellularis. In addition, caspase-3, terminal deoxyuridine nick-end labeling assay, and electron microscopy were performed in ileum samples. Macroscopic and histologic lesions suggestive of PE were first detected 11 days after infection and continued through day 24. L. intracellularis antigen was first detected in the intestine by IHC on day 5 after inoculation, and the bacterium was first detected by transmission electron microscopy on day 15. Positive IHC staining for [L. intracellularis] and enterocyte proliferation, but no gross lesion, were detected on day 29. All 3 pigs euthanized on day 35 were grossly and histologically normal and IHC negative. Hyperplastic crypts in challenge pigs had more apoptotic cells on days 15, 19, and 24 postinfection ( P < .05) compared to control pigs. Our results demonstrated the progression of lesions and infection by L. intracellularis and that inhibition of enterocyte apoptosis is not involved in the pathogenesis of proliferative enteropathy.


Assuntos
Infecções por Desulfovibrionaceae/veterinária , Lawsonia (Bactéria) , Doenças dos Suínos/microbiologia , Animais , Apoptose , Estudos de Casos e Controles , Infecções por Desulfovibrionaceae/patologia , Progressão da Doença , Enterócitos/microbiologia , Enterócitos/patologia , Feminino , Trato Gastrointestinal/microbiologia , Trato Gastrointestinal/patologia , Íleo/patologia , Íleo/ultraestrutura , Masculino , Microscopia Eletrônica de Transmissão/veterinária , Suínos , Doenças dos Suínos/patologia
4.
Foodborne Pathog Dis ; 12(8): 704-11, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26217917

RESUMO

Enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for neonatal diarrhea, postweaning diarrhea, and edema disease in pigs. Although it can be harmless, ETEC is also present in the intestines of other animal species and humans, causing occasional diarrhea outbreaks. The evaluation of this pathogen's presence in food sources is becoming an increasingly important issue in human health. In order to determine the prevalence of ETEC in nondiarrheic pigs, 990 animals from 11 pig farms were sampled. Using end-time polymerase chain reaction (PCR), eltA, estI genes, or both, were detected in 150 (15.2%) animals. From the positive samples, 40 (26.6%) ETEC strains were isolated, showing 19 antibiotic-resistance patterns; 52.5% of these strains had multiple antibiotic resistances, and 17.5% carried the intI2 gene. The most prevalent genotypes were rfb(O157)/estII/aidA (32.5%) and estI/estII (25.0%). The estII gene was identified most frequently (97.5%), followed by estI (37.5%), astA (20.0%), and eltA (12.5%). The genes coding the fimbriae F5, F6, and F18 were detected in three single isolates. The aidA gene was detected in 20 ETEC strains associated with the estII gene. Among the isolated ETEC strains, stx(2e)/estI, stx(2e)/estI/estII, and stx(2e)/estI/estII/intI2 genotypes were identified. The ETEC belonged to 12 different serogroups; 37.5% of them belonged to serotype O157:H19. Isolates were grouped by enterobacterial repetitive intergenic consensus-PCR into 5 clusters with 100.0% similarity. In this study, we demonstrated that numerous ETEC genotypes cohabit and circulate in swine populations without clinical manifestation of neonatal diarrhea, postweaning diarrhea, or edema disease in different production stages. The information generated is important not only for diagnostic and epidemiological purposes, but also for understanding the dynamics and ecology of ETEC in pigs in different production stages that can be potentially transmitted to humans from food animals.


Assuntos
Anti-Infecciosos/análise , Farmacorresistência Bacteriana Múltipla/genética , Escherichia coli Enterotoxigênica/isolamento & purificação , Genes Bacterianos , Carne Vermelha/microbiologia , Animais , Anti-Infecciosos/farmacologia , DNA Bacteriano/genética , Diarreia/microbiologia , Diarreia/veterinária , Edematose Suína/microbiologia , Escherichia coli Enterotoxigênica/efeitos dos fármacos , Escherichia coli Enterotoxigênica/genética , Proteínas de Escherichia coli/genética , Contaminação de Alimentos , Microbiologia de Alimentos , Técnicas de Genotipagem , Carne Vermelha/análise , Suínos , Doenças dos Suínos/microbiologia
5.
Rev Argent Microbiol ; 44(2): 85-8, 2012.
Artigo em Espanhol | MEDLINE | ID: mdl-22997765

RESUMO

The purpose of this work was to characterize 47 Escherichia coli strains isolated from 32 pigs diagnosed with postweaning diarrhea and three pigs with edema disease by PCR. Forty two (95.5 %) of the strains isolated from diarrheic pigs were characterized as enterotoxigenic E. coli (ETEC) and 2 (4.5 %) as Shiga toxin-producing E. coli (STEC). Fourteen (33.3 %) ETEC strains were positive for est/estII/fedA genes. The most complex genotype was eltA/estI/faeG/aidA. Strains isolated from pigs with ED were classified as porcine STEC and were stx2e/aidA carriers. Eleven (25 %) strains carried the gene encoding adhesin protein AIDA-I. However, genes coding for F5, F6, F41, intimin and Paa were not detected. The development of vaccines generating antibodies against prevalent E. coli adhesins in Argentina could be useful for the prevention of PWD and ED.


Assuntos
Diarreia/veterinária , Edematose Suína/microbiologia , Escherichia coli Enterotoxigênica/genética , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/genética , Genes Bacterianos , Escherichia coli Shiga Toxigênica/genética , Doenças dos Suínos/microbiologia , Adesinas de Escherichia coli/genética , Animais , Argentina/epidemiologia , Diarreia/epidemiologia , Diarreia/microbiologia , Surtos de Doenças , Edematose Suína/epidemiologia , Escherichia coli Enterotoxigênica/isolamento & purificação , Enterotoxinas/genética , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Genótipo , Escherichia coli Shiga Toxigênica/isolamento & purificação , Sus scrofa , Suínos , Doenças dos Suínos/epidemiologia , Desmame
6.
Rev. argent. microbiol ; Rev. argent. microbiol;44(2): 85-88, jun. 2012. tab
Artigo em Espanhol | LILACS | ID: lil-657616

RESUMO

El objetivo del trabajo fue caracterizar mediante PCR 47 aislamientos de Escheríchia coli recuperados de 32 cerdos con diagnóstico clínico de diarrea posdestete (DPD) y de 3 cerdos con enfermedad de los edemas (ED). Sobre 44 aislamientos provenientes de cerdos con DPD, 42 (95,5 %) fueron caracterizados como E. coli enterotoxigénicos (ETEC) y 2 (4,5 %) como E. coli productores de toxina Shiga (STEC). Catorce aislamientos de ETEC (33,3 %) fueron positivos para los genes estl/estlI/fedA. El genotipo más complejo fue eltA/estll/east1/faeG/aidA. Los aislamientos provenientes de cerdos con ED se clasificaron como STEC porcinos y fueron portadores de stxJaidA. Once aislamientos (25 %) fueron portadores del gen que codifica la expresión de la adhesina AIDA-I. Sin embargo, en ningún aislamiento se detectaron los genes que codifican la expresión de las adhesinas F5, F6, F41, de intimina y de "Paa". La prevención de la DPD y de la ED podría realizarse mediante el desarrollo de vacunas que generen anticuerpos contra las adhesinas de las cepas de E. coli prevalentes en la Argentina.


The purpose of this work was to characterize 47 Escherichia coli strains isolated from 32 pigs diagnosed with postweaning diarrhea and tree pigs with edema disease by PCR. Forty two (95.5 %) of the strains isolated from diarrheic pigs were characterized as enterotoxigenic E. coli (ETEC) and 2 (4.5 %) as Shiga toxin-producing E. coli (STEC). Fourteen (33.3 %) ETEC strains were positive for est/estll/fedA genes. The most complex genotype was eltA/estl/faeG/aidA. Strains isolated from pigs with ED were classified as porcine STEC and were stxjaidA carriers. Eleven (25 %) strains carried the gene encoding adhesln protein AIDA-I. However, genes coding for F5, F6, F41, intimin and Paa were not detected. The development of vaccines generating antibodies against prevalent E. coli adhesins in Argentina could be useful for the prevention of PWD and ED.


Assuntos
Animais , Diarreia/veterinária , Edematose Suína/microbiologia , Escherichia coli Enterotoxigênica/genética , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/genética , Genes Bacterianos , Escherichia coli Shiga Toxigênica/genética , Doenças dos Suínos/microbiologia , Adesinas de Escherichia coli/genética , Argentina/epidemiologia , Surtos de Doenças , Diarreia/epidemiologia , Diarreia/microbiologia , Edematose Suína/epidemiologia , Escherichia coli Enterotoxigênica/isolamento & purificação , Enterotoxinas/genética , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Genótipo , Sus scrofa , Suínos , Escherichia coli Shiga Toxigênica/isolamento & purificação , Doenças dos Suínos/epidemiologia , Desmame
7.
Rev. argent. microbiol ; Rev. argent. microbiol;44(2): 85-88, jun. 2012. tab
Artigo em Espanhol | BINACIS | ID: bin-129222

RESUMO

El objetivo del trabajo fue caracterizar mediante PCR 47 aislamientos de Escheríchia coli recuperados de 32 cerdos con diagnóstico clínico de diarrea posdestete (DPD) y de 3 cerdos con enfermedad de los edemas (ED). Sobre 44 aislamientos provenientes de cerdos con DPD, 42 (95,5 %) fueron caracterizados como E. coli enterotoxigénicos (ETEC) y 2 (4,5 %) como E. coli productores de toxina Shiga (STEC). Catorce aislamientos de ETEC (33,3 %) fueron positivos para los genes estl/estlI/fedA. El genotipo más complejo fue eltA/estll/east1/faeG/aidA. Los aislamientos provenientes de cerdos con ED se clasificaron como STEC porcinos y fueron portadores de stxJaidA. Once aislamientos (25 %) fueron portadores del gen que codifica la expresión de la adhesina AIDA-I. Sin embargo, en ningún aislamiento se detectaron los genes que codifican la expresión de las adhesinas F5, F6, F41, de intimina y de "Paa". La prevención de la DPD y de la ED podría realizarse mediante el desarrollo de vacunas que generen anticuerpos contra las adhesinas de las cepas de E. coli prevalentes en la Argentina.(AU)


The purpose of this work was to characterize 47 Escherichia coli strains isolated from 32 pigs diagnosed with postweaning diarrhea and tree pigs with edema disease by PCR. Forty two (95.5 %) of the strains isolated from diarrheic pigs were characterized as enterotoxigenic E. coli (ETEC) and 2 (4.5 %) as Shiga toxin-producing E. coli (STEC). Fourteen (33.3 %) ETEC strains were positive for est/estll/fedA genes. The most complex genotype was eltA/estl/faeG/aidA. Strains isolated from pigs with ED were classified as porcine STEC and were stxjaidA carriers. Eleven (25 %) strains carried the gene encoding adhesln protein AIDA-I. However, genes coding for F5, F6, F41, intimin and Paa were not detected. The development of vaccines generating antibodies against prevalent E. coli adhesins in Argentina could be useful for the prevention of PWD and ED.(AU)


Assuntos
Animais , Diarreia/veterinária , Edematose Suína/microbiologia , Escherichia coli Enterotoxigênica/genética , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/genética , Genes Bacterianos , Escherichia coli Shiga Toxigênica/genética , Doenças dos Suínos/microbiologia , Adesinas de Escherichia coli/genética , Argentina/epidemiologia , Diarreia/epidemiologia , Diarreia/microbiologia , Surtos de Doenças , Edematose Suína/epidemiologia , Escherichia coli Enterotoxigênica/isolamento & purificação , Enterotoxinas/genética , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Genótipo , Escherichia coli Shiga Toxigênica/isolamento & purificação , Sus scrofa , Suínos , Doenças dos Suínos/epidemiologia , Desmame
8.
Braz. j. vet. pathol ; 5(1): 25-30, mar. 2012. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1397656

RESUMO

An outbreak of proliferative hemorrhagic enteropathy in a commercial pig farm, characterized by granulomatous enteritis, was studied by slaughter checks and by histopathological, histochemical and immunohistochemical studies. Six of the postmortem examined pigs (117-122-day-old) with diagnosis of porcine proliferative enteropathy (PPE) showed granulomatous enteritis and 4.3% of the 465 slaughtered pigs showed gross lesions of PPE. A total of 33 of the 66 ileum samples had histopathological changes, whereas 17 of them had granulomatous enteritis and PPE lesions. Lawsonia intracellularis was immunolabeled in 52 of the ileum samples in epithelial cells and within granulomatous inflammation in Peyer´s patches and in 28 samples of mesenteric lymph nodes. Porcine circovirus type 2 was only detected by immunohistochemistry in 4 ileum samples and in 5 mesenteric lymph nodes. Since there was a strong statistically significant association between granulomatous enteritis and L. intracellularis infection, granulomatous enteritis could be considered as a part of the PPE complex.(AU)


Assuntos
Animais , Suínos , Doença de Crohn/veterinária , Gastroenterite/veterinária , Ileíte/veterinária , Imuno-Histoquímica , Lawsonia (Bactéria) , Infecções por Desulfovibrionaceae , Enteropatias/veterinária
9.
J Gen Virol ; 92(Pt 12): 2871-2878, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21849519

RESUMO

Sporadic outbreaks of human H3N2 influenza A virus (IAV) infections in swine populations have been reported in Asia, Europe and North America since 1970. In South America, serological surveys in pigs indicate that IAVs of the H3 and H1 subtypes are currently in circulation; however, neither virus isolation nor characterization has been reported. In November 2008, an outbreak of respiratory disease in pigs consistent with swine influenza virus (SIV) infection was detected in Argentina. The current study describes the clinical epidemiology, pathology, and molecular and biological characteristics of the virus. Phylogenetic analysis revealed that the virus isolate shared nucleotide identities of 96-98 % with H3N2 IAVs that circulated in humans from 2000 to 2003. Antigenically, sera from experimentally inoculated animals cross-reacted mainly with non-contemporary human-origin H3N2 influenza viruses. In an experimental infection in a commercial swine breed, the virus was of low virulence but was transmitted efficiently to contact pigs and caused severe disease when an infected animal acquired a secondary bacterial infection. This is the first report of a wholly human H3N2 IAV associated with clinical disease in pigs in South America. These studies highlight the importance of two-way transmission of IAVs and SIVs between pigs and humans, and call for enhanced influenza surveillance in the pig population worldwide.


Assuntos
Vírus da Influenza A Subtipo H3N2/patogenicidade , Influenza Humana/transmissão , Influenza Humana/virologia , Infecções por Orthomyxoviridae/transmissão , Infecções por Orthomyxoviridae/veterinária , Animais , Argentina/epidemiologia , Surtos de Doenças , Humanos , Vírus da Influenza A Subtipo H3N2/isolamento & purificação , Influenza Humana/epidemiologia , Dados de Sequência Molecular , Infecções por Orthomyxoviridae/epidemiologia , Infecções por Orthomyxoviridae/virologia , Filogenia , Filogeografia , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/transmissão , Doenças dos Suínos/virologia , Replicação Viral
11.
Foodborne Pathog Dis ; 6(8): 965-72, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19642916

RESUMO

The aim of this surveillance was to study both Salmonella spp. shedding patterns and the time course of serological response in farrow-to-finish reared pigs from a subclinically infected farm. Antimicrobial resistance profile, molecular subtyping, and the relationship among the isolates were determined by pulsed-field gel electrophoresis (PFGE). A farrow-to-finish farm of 6000 sows, with a history of Salmonella Typhimurium septicemia, was selected. A longitudinal bacteriological and serological study was conducted in 25 sows before farrowing (M/S1) and in 50 offspring at 21 (M/S2), 35 (M/S3), 65 (M/S4), 86 (M/S5), 128 (M/S6), and 165 (M/S7) days of age. Serum antibodies were tested using Herdcheck((R)) Swine Salmonella antibody test kit (Idexx Laboratories, ME). Bacteria were isolated from pooled fecal samples. Suspected isolates were confirmed by conventional biochemical assays, and those identified as Salmonella spp. were serotyped. A variation between seropositive percentages and positive fecal samples was observed. Serologically positive pigs decreased from S1 to S4, and subsequently increased from S4 to S7. The percentages of fecal positive culture increased from M1 to M3, and then declined in M4, increased in M5, and were negative in M6 and M7. In the study three serovars, Salmonella 3,10:e,h:-, Salmonella Muenster, and Salmonella Bovismorbificans, were identified with low pathogenicity for swine. Three multidrug resistance strains (one belonged to Salmonella 3,10:e,h:- and two belonged to Salmonella Muenster) were found. PFGE results showed three different but closely related patterns among the 13 isolates of Salmonella Bovismorbificans, and two patterns for the three Salmonella Muenster and Salmonella 3,10:e,h:- isolates. This longitudinal study established critical points of Salmonella spp. infection in the farm and the production stages, where appropriate control measures must be taken. PFGE showed clonal relationships in each serovar. Antibiotic resistance profiles should be periodically included due to public health concerns.


Assuntos
Farmacorresistência Bacteriana/genética , Salmonelose Animal/microbiologia , Salmonella enterica , Doenças dos Suínos/microbiologia , Envelhecimento , Criação de Animais Domésticos/métodos , Animais , Argentina/epidemiologia , Derrame de Bactérias , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Eletroforese em Gel de Campo Pulsado/veterinária , Fezes/microbiologia , Feminino , Variação Genética , Estudos Longitudinais , Masculino , Filogenia , Reto/microbiologia , Salmonelose Animal/epidemiologia , Salmonelose Animal/prevenção & controle , Salmonella enterica/classificação , Estudos Soroepidemiológicos , Sorotipagem/veterinária , Suínos , Doenças dos Suínos/epidemiologia , Fatores de Tempo
12.
Braz. j. vet. pathol ; 2(1): 8-11, 2009. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1435540

RESUMO

The objectives of this work were: 1) to investigate L. intracellularis antibodies in grower-finisher pigs from 30 herds by immunofluorescent antibody test (IFAT) 2) to evaluate the porcine proliferative enterophaty (PPE) lesions by histopathology, histochemistry (Warthin Starry) and immunohistochemistry (IHC) in ileum samples obtained at slaughterhouse from 10 selected herds, 9 seropositive and 1 control seronegative. At each farm, 30 grower-finisher pigs (18 to 20 weeks old pigs) were randomly selected for blood sampling. The sera were analyzed with a commercial IFAT kit (IleiTest, Elanco Animal Health). The overall percentage of positive pigs was 31.2% (281/900), whereas the percentage of positive farms was 76.7% (23/30). The within-herd mean percentage of positive sera ranged from 27.5 to 53.6%. Only 1 out of the 10 selected farms showed characteristic gross and histopathological lesions at slaughterhouse. On the other hand, 4 farms were positive by immunohistochemistry. In conclusion L. intracellularis infection is widespread among Argentinean farms even though the within-herd percentage was quite variable. Slaughter pigs without microscopic lesions showed immunoreactivity to L. intracellularis.


Assuntos
Animais , Suínos/microbiologia , Lawsonia (Bactéria)/isolamento & purificação , Infecções por Desulfovibrionaceae/epidemiologia , Enteropatias/veterinária , Argentina , Testes Sorológicos/veterinária
13.
Braz. j. vet. pathol ; 2(1): 16-22, 2009. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1435571

RESUMO

An experimental study was performed in order to analyze the histopathological findings of gastrointestinal mucosa and regional lymph-nodes in piglets intoxicated with T-2 toxin as well as the mechanism of T-2 induced apoptosis in the intestinal crypt epithelia. Sixteen piglets were divided into 4 groups receiving a single oral administration of T-2 toxin. Group A: 2.5 mg T-2/kg b.w.; group B: 2 mg T-2/kg b.w.; group C: 1.5 mg T-2/kg b.w.; group D: control group without toxin. Organ lesions were classified in grades 0 to 4 according with severity and were statistically analyzed. Apoptosis was examined in intestinal sections only in groups A and D. Group A piglets showed clinical signs and pathological changes related with those seen in circulatory shock. Microscopic lesions were observed only in pigs from groups A and B. In stomach, lesions consisted of congestion, hemorrhages and necrotic cells in the isthmus and neck regions. Submucosal edema and necrotic crypt epithelial cells were seen in duodenum, jejunum, ileum, cecum and colon. The greatest grade of lesions was observed in colon. In lymph node, grade of lymphocytes depletion and necrosis was significantly higher in cortex than paracortex. Apoptotic bodies were present in intestinal crypt cells, lymphoid cells from lamina propria and ileal Peyer's patches. Apoptosis seems to be the mechanism involved in the development of intestinal lesions.


Assuntos
Animais , Suínos , Toxina T-2/toxicidade , Apoptose , Mucosa Gástrica/anatomia & histologia , Imuno-Histoquímica/veterinária
14.
Braz. j. vet. pathol ; 2(1): 35-40, 2009. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1435589

RESUMO

Clostridium difficile is a Gram-positive opportunistic anaerobic bacterium found in the soil, water as well as the digestive tract of several species of mammals. Currently in high-health herds and not related with antibiotic treatment, C. difficile has been emerged in association with neonatal catarrhal, fibrinous or purulent colitis in 2 to 7 day-old piglets. Mesocolon edema and colitis with a "volcano" lesion e pathological key marks of C. difficile infection. In farm 1, the prevalence of neonatal piglet mesocolon edema and colitis was evaluated in an extensive study of postmortem preweaning mortality. Lectinhistochemistry pattern of normal colon and colon with edema and colitis were analyzed in order to provide more accurate information related with pathogenesis of C.difficile infection. In farm 2, a clinical description of an outbreak of neonatal colitis in piglets due to C. difficile infection was reported. A total 820 piglets were post-mortem examined, from them, 8 cases were classified as suspected of C. difficile infection (0.1%). Age of affected piglets varied between 3 to 14 days. In all of them, the key mark was the severe mesocolon edema and 2 cases were characterized by focal necrosis and loss of epithelial cells associated with a focal infiltration of neutrophils and macrophages in the lamina propria and lumen like erupted volcano. Lectins SBA and DBA that has affinity to a Gal epitope were negative in normal samples but reacted strongly positive at the glycocalix of the villi enterocytes of the affected samples indicating a change in the carbohydrates pattern of the cells surfaces that might favor the binding of C. difficile toxin A (TcdA). In farm 2, from 11 examined piglets, 6 of them (54%) showed different degree of mesocolon edema and in only 2 of them; volcano lesions were the main histopathological findings. Rectal swab from 8 diarrheic and 5 postmortem examined piglets were surveyed for eltA estl estll and stx2, virulence gens of E. coli and all samples were negative. Clostridium difficile toxins A and B were identified only in the sample with severe gross and microscopic changes. Isolation of difficile was unsuccessfully. Infection of C. difficile is present in Argentinean pig farms. Diagnosis of subclinical infection might be negligent if post-mortem studies of preweaning mortality at weekly intervals are not performed. However, when clinical disease appeared, mortality might be high as it was reported in the farm 2. Comparative lectinhistochemistry studies from field cases added further information of the carbohydrates present on glycocalix of villi enterocytes related with toxin receptors.


Assuntos
Animais , Suínos/microbiologia , Infecções por Clostridium/veterinária , Colite/veterinária , Edema/veterinária , Mesocolo/patologia , Animais Recém-Nascidos/microbiologia
15.
Emerg Infect Dis ; 14(3): 484-6, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18325268

RESUMO

We describe an outbreak of vomiting, wasting, and encephalomyelitis syndrome in piglets in Argentina, caused by porcine hemagglutinating encephalomyelitis coronavirus (PHE-CoV) infection. Diagnosis was made by epidemiologic factors, pathologic features, immunohistochemistry, reverse transcription-PCR, and genomic sequencing. This study documents PHE-CoV infection in South America.


Assuntos
Infecções por Coronavirus/veterinária , Coronavirus/isolamento & purificação , Doenças dos Suínos/virologia , Animais , Argentina/epidemiologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Surtos de Doenças/veterinária , Suínos , Doenças dos Suínos/epidemiologia
16.
Pesqui. vet. bras ; Pesqui. Vet. Bras. (Online);24(1): 1-5, jan.-mar. 2004. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-3182

RESUMO

A eperitrozoonose suína é uma doença hemotrópica causada por Eperitrozoon suis, atualmente denominado Mycoplasma suis, uma bactéria extracelular que, aparentemente, adere à membrana dos eritrócitos suínos, induzindo sua deformação e lesionando-os. O presente trabalho busca estabelecer os aspectos estruturais e ultra-estruturais, pouco conhecidos, deste microorganismo. O estudo ultra-estrutural revelou a presença de estruturas correspondentes a túbulos disseminados no soma bacteriano. Observou-se também uma separação variável entre a membrana do microorganismo e a parede do eritrócito. O estudo morfométrico e a localização de M. suis pode permitir especulação sobre seu mecanismo de ação. (AU)


Swine eperythrozoonosis is a haemotrophic disease caused by Eperythrozoon suis, actually called Mycoplasma suis, an extracellular bacterial organism that apparently adheres to pig erythrocyte membrane, inducing its deformation and damage. Since little is known about the ultrastructural and morphometrical aspects of this microorganism, the present work aimed to deal with these issues. The ultrastructural study revealed the presence of structures corresponding to tubules disseminated throughout the soma of M. suis. A variable separation between the microorganism membrane and that of the erythrocyte was also observed. The structural and positional attitude of M. suis could allow speculation about its mechanism of action.(AU)


Assuntos
Animais , Masculino , Feminino , Infecções por Mycoplasma , Suínos
17.
Pesqui. vet. bras ; Pesqui. Vet. Bras. (Online);24(1): 1-5, jan.-mar. 2004. ilus, tab
Artigo em Inglês | LILACS | ID: lil-364147

RESUMO

A eperitrozoonose suína é uma doença hemotrópica causada por Eperitrozoon suis, atualmente denominado Mycoplasma suis, uma bactéria extracelular que, aparentemente, adere à membrana dos eritrócitos suínos, induzindo sua deformação e lesionando-os. O presente trabalho busca estabelecer os aspectos estruturais e ultra-estruturais, pouco conhecidos, deste microorganismo. O estudo ultra-estrutural revelou a presença de estruturas correspondentes a túbulos disseminados no soma bacteriano. Observou-se também uma separação variável entre a membrana do microorganismo e a parede do eritrócito. O estudo morfométrico e a localização de M. suis pode permitir especulação sobre seu mecanismo de ação.


Assuntos
Animais , Masculino , Feminino , Infecções por Mycoplasma , Suínos
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