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Cardiovasc Hematol Agents Med Chem ; 18(2): 124-134, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32660409


OBJECTIVE: The aim of the present study is to isolate and characterize the bioactive compounds from Pleurotus djamor against human breast cancer (MDA-MD-231) and mouse T cell lymphoma (EL4) cell lines. MATERIALS AND METHODS: Sequential fractionization and column chromatography methods were involved in compound isolation. The structures of the isolated compound were determined by NMR, GC/MS, and X-ray crystallography studies. RESULTS: The isolated compounds 1- 4 [D-mannitol (C1), ergosta-5,7,22-trien-3ß-ol (C2), 5,8- epidioxy-ergosta-6-22-dien-3ß-ol (C3), and palmitic acid (C4)] are white crystal and amorphous powder in nature. All these compounds were isolated from this mushroom for the first time. In vitro lipid peroxidation activities of isolated compounds were determined by ferric thiocyanate (FTC) and thiobarbituric acid (TBA) method. The sterol derivatives C2 and C3 compounds displayed strong antioxidant activity and were not significantly different (p<0.05) to α-tocopherol. This finding elaborates on the isolation of a cytotoxic compound C2 and C3 from P. djamor via a rapid elution method. CONCLUSION: The compound C3 has exhibited better cytotoxic activity against MDA-MD-231 and EL4 cells. The present finding and data might provide new insights into the possible therapeutic and pharmaceutical use for the design of anti-cancer drugs from this edible mushroom.

Antineoplásicos/química , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Pleurotus/química , Esteróis/química , Esteróis/farmacologia , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Neoplasias/tratamento farmacológico , Esteróis/isolamento & purificação
Carbohydr Res ; 346(15): 2362-7, 2011 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-21903205
Carbohydr Res ; 345(14): 1988-97, 2010 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-20688320


Regioselective facile one-pot synthesis of 16 different sugar-based quinoline, naphthyridine, and xanthone derivatives is reported. The compounds are characterized by NMR spectroscopy and elemental analysis. The beta-Anomeric form of the sugar moiety was identified from (1)H NMR studies. Antimicrobial studies of these sugar-heterocyclic derivatives, 3a, 3b, 3f, 5c, 7a, 7b, and 7c show excellent activity against different microbes.

Anti-Infecciosos/síntese química , Naftiridinas/síntese química , Quinolinas/síntese química , Xantonas/síntese química , Anti-Infecciosos/farmacologia , Glicosídeos/química , Lactonas/química , Testes de Sensibilidade Microbiana , Naftiridinas/farmacologia , Ressonância Magnética Nuclear Biomolecular , Xantonas/farmacologia
Carbohydr Res ; 345(12): 1649-57, 2010 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-20579978


BF(3).Et(2)O-catalysed O-glycosylation of 1,2,3-tri-O-acetyl-4,6-O-butylidene- and ethylidene-beta-d-glucopyranose with different aliphatic and aromatic alcohols proceeds for the most part with complete retention of anomeric configuration. Antioxidant activity of O-glycosides shows significant inhibition (IC(50) approximately 77%). 1,3-Dipolar cycloaddition of terminal alkyne derivatives of O-glycosides with glycosyl azide results in disaccharides.

Antioxidantes/síntese química , Dissacarídeos/síntese química , Glicosídeos/síntese química , Álcoois/química , Alcinos/química , Antioxidantes/química , Azidas/química , Boranos/química , Catálise , Ciclização , Dissacarídeos/química , Óxido de Etileno/química , Glicosídeos/química , Glicosilação , Conformação Molecular , Estereoisomerismo
Indian J Exp Biol ; 47(4): 289-97, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19382726


Casuarina equisetifolia is one of the ecologically and economically important tropical coastal trees nodulated by nitrogen-fixing actinomycete Frankia and forming symbiotic associations with both ecto- and endomycorrhizal fungi. The present study aims at the ultrastructural study of interactions between C. equisetifolia, Frankia, and mycorrhiza. C. equisetifolia seeds were sterilised and germinated under in vitro condition. The seedlings were transferred to conical flasks containing vermiculite and saw dust with Hoagland's solution. After 30 days, the inoculum of AM fungus--Glomusfasciculatum (A), ectomycorrhizal fungus-Pisolithus tinctorius (E) and actinorhizal Frankia (F) were inoculated individually and in various combinations, (A+E), (A+F), E+F) and (A+E+F). After 90 days, the experimental plant roots and nodules were harvested for assessment of growth characters of mycorrhizal and actinorhizal association by light and scanning electron microscope methods. C. equisetifolia roots were infected with arbuscles and vesicles of G. fasciculatum; P. tinctorius formed fungal sheath but no Hartig net. Large number of cortical cells were seen infected with Frankia, hyphae of Frankia were frequently seen penetrating from cell to cell directly through cell walls and Frankia occupied majority of the cell volume.

Basidiomycota/fisiologia , Frankia/fisiologia , Glomeromycota/fisiologia , Magnoliopsida/microbiologia , Micorrizas/fisiologia , Nódulos Radiculares de Plantas/microbiologia , Nódulos Radiculares de Plantas/ultraestrutura , Frankia/ultraestrutura , Hifas/ultraestrutura