Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 56
Filtrar
1.
Braz. j. biol ; 82: e239219, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153471

RESUMO

Abstract Salmo trutta fario is the most important fish species of family Salmonidae inhibited in cool waters all over the world including the nortern parts of Pakistan. This study was aimed to find out the prey selection and feeding habits of the species. One hundred and eighty five Salmo trutta fario were captured from March 2016 to April 2017. Feeding intensity as represented by a fullness index (FI), changing with various length groups and season. Out of 185 Brown trout 24 fish stomaches were found empty. The most important and common food items of brown trout were Brachycentridae, Blepharocera, Hydropsychidae, ephemerella spp. Kruskal Wallis H-test were applied on feeding intensity groups consisting of three month group. The test statistic for K.W-H-test were (H=8.13 with df =3) had a p-value of 0.043 < (alpha=0.05 indicates favor of the alternative hypothesis of at least one difference among the feeding intensity groups. The linear relationship of N with index of relative importance and %IRI denoted by least square regression line (N = 35.2 + 22.1%IRI), shows that for 22 prey there is 1% change in IRI. The relationship between total length (mm) and fish body weight (gm) is expressed by Pearson correlation coefficient (r = 0.976), showed that total length (mm) and fish body weight (gm.) is highly correlated. Descriptive statistics are used for the stomach fullness, which shows that feeding intensity was recorded higher from March to May. A total of 2289 preys was recorded including the most common were; Trichoptera, Hydropsychidae, Brachycentridae, diptera, blepharocera, ephemerala, chironomida, honey bees, grass hopper, locust, trout egg, trochanter, plant tissue, stones were retrieved from the gut contents of brown trout. According to index of relative importance IRI% four preys represents major components of the diet. The highest IRI% was recorded in Brachycentridae (39.38%), followed by blepharocera (13.23%), Hydropsychidae (10.76%) and ephemerella spp (8.28%). The relationship between IRI and FO is (r =0.556) is moderately correlated with coefficient of determination (r2=0.31). This study will help in the development of an artificial diet for the species for better growth performance in captivity.


Resumo Salmo trutta fario é a espécie de peixe mais importante da família dos salmonídeos, inibida em águas frias em todo o mundo, incluindo as partes do noroeste do Paquistão. Este estudo teve como objetivo conhecer a seleção de presas e os hábitos alimentares da espécie. Cento e oitenta e cinco Salmo trutta fario foram capturados de março de 2016 a abril de 2017. Intensidade de alimentação representada por um índice de plenitude (FI), mudando com vários grupos de comprimento e estação. De 185 trutas, 24 estômagos de peixes foram encontrados vazios. Os itens alimentares mais importantes e comuns da truta marrom foram brachycentridae, blepharocera, hydropsychidae, ephemerellaspp. O teste H de Kruskal Wallis foi aplicado em grupos de intensidade de alimentação consistindo de um grupo de três meses. A estatística de teste para o teste KW-H foi (H = 8,13 com df = 3) teve um valor de p de 0,043 <(alfa = 0,05 indica favor da hipótese alternativa de pelo menos uma diferença entre os grupos de intensidade de alimentação. relação de N com o índice de importância relativa e% IRI denotado pela linha de regressão do mínimo quadrado (N = 35,2 + 22,1% IRI), mostra que para 22 presas há 1% de mudança no IRI. A relação entre comprimento total (mm) e peixes o peso corporal (gm) é expresso pelo coeficiente de correlação de Pearson (r = 0,976), mostrou que o comprimento total (mm) e o peso corporal dos peixes (gm.) são altamente correlacionados. Estatísticas descritivas são usadas para a plenitude do estômago, o que mostra que a intensidade da alimentação foi registrado mais alto de março a maio. Um total de 2.289 presas foi registrado, incluindo os mais comuns foram; trichoptera, hydropsychidae, brachycentridae, diptera, blepharocera, efemérala, chironomida, abelhas, funil de grama, gafanhoto, ovo de truta, trocânter, tecido vegetal, pedras foram retiradas do intestino conteúdo de truta marrom. De acordo com o índice de importância relativa IRI%, quatro presas representam os principais componentes da dieta. O maior IRI% foi registrado em brachycentridae (39,38%), seguido por blepharocera (13,23%), hydropsychidae (10,76%) e ephemerellaspp (8,28%). A relação entre IRI e FO é (r = 0,556) está moderadamente correlacionada com o coeficiente de determinação (r2 = 0,31). Este estudo auxiliará no desenvolvimento de uma dieta artificial para a espécie para melhor desempenho de crescimento em cativeiro.

2.
Braz J Biol ; 82: e239219, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33787749

RESUMO

Salmo trutta fario is the most important fish species of family Salmonidae inhibited in cool waters all over the world including the nortern parts of Pakistan. This study was aimed to find out the prey selection and feeding habits of the species. One hundred and eighty five Salmo trutta fario were captured from March 2016 to April 2017. Feeding intensity as represented by a fullness index (FI), changing with various length groups and season. Out of 185 Brown trout 24 fish stomaches were found empty. The most important and common food items of brown trout were Brachycentridae, Blepharocera, Hydropsychidae, ephemerella spp. Kruskal Wallis H-test were applied on feeding intensity groups consisting of three month group. The test statistic for K.W-H-test were (H=8.13 with df =3) had a p-value of 0.043 < (alpha=0.05 indicates favor of the alternative hypothesis of at least one difference among the feeding intensity groups. The linear relationship of N with index of relative importance and %IRI denoted by least square regression line (N = 35.2 + 22.1%IRI), shows that for 22 prey there is 1% change in IRI. The relationship between total length (mm) and fish body weight (gm) is expressed by Pearson correlation coefficient (r = 0.976), showed that total length (mm) and fish body weight (gm.) is highly correlated. Descriptive statistics are used for the stomach fullness, which shows that feeding intensity was recorded higher from March to May. A total of 2289 preys was recorded including the most common were; Trichoptera, Hydropsychidae, Brachycentridae, diptera, blepharocera, ephemerala, chironomida, honey bees, grass hopper, locust, trout egg, trochanter, plant tissue, stones were retrieved from the gut contents of brown trout. According to index of relative importance IRI% four preys represents major components of the diet. The highest IRI% was recorded in Brachycentridae (39.38%), followed by blepharocera (13.23%), Hydropsychidae (10.76%) and ephemerella spp (8.28%). The relationship between IRI and FO is (r =0.556) is moderately correlated with coefficient of determination (r2=0.31). This study will help in the development of an artificial diet for the species for better growth performance in captivity.


Assuntos
Rios , Truta , Animais , Abelhas , Dieta , Hábitos , Paquistão
3.
ACS Nano ; 15(1): 1331-1341, 2021 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-33395272

RESUMO

The biofunctionalization of particles with specific targeting moieties forms the foundation for molecular recognition in biomedical applications such as targeted nanomedicine and particle-based biosensing. To achieve a high precision of targeting for nanomedicine and high precision of sensing for biosensing, it is important to understand the consequences of heterogeneities of particle properties. Here, we present a comprehensive methodology to study with experiments and simulations the collective consequences of particle heterogeneities on multiple length scales, called superpositional heterogeneities, in generating reactivity variability per particle. Single-molecule techniques are used to quantify stochastic, interparticle, and intraparticle variabilities, in order to show how these variabilities collectively contribute to reactivity variability per particle, and how the influence of each contributor changes as a function of the system parameters such as particle interaction area, the particle size, the targeting moiety density, and the number of particles. The results give insights into the consequences of superpositional heterogeneities for the reactivity variability in biomedical applications and give guidelines on how the precision can be optimized in the presence of multiple independent sources of variability.

4.
Comput Methods Programs Biomed ; 190: 105350, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32078958

RESUMO

Background and Objective The positivity property of the non-linear dynamical systems is one of the essential features in different fields of bio-medical engineering, science and many more. The state variables, involving in the models, describing the natural phenomenon such as concentration, density and population size etc. must be positive. Therefore, the computing techniques used to solve the system of non-linear differential equations must be consisted with the continuous nature of the models. But, unfortunately there are some existing techniques in the literature that do not preserve the positivity property, especially for the multi-space dimensional models. So there is a gap in the literature that should be filled up, by constructing the positivity preserving numerical algorithms. In this study, we consider a susceptible-infected-recovered (SIR) reaction diffusion epidemic model in two space dimensions from biomedical engineering and solved numerically to observe the behavior of the model. Since the state variables involved in this system are population densities therefore we design a novel computational method which is time efficient because of its splitting structure and holds the positivity as well as other important structure of epidemic system. Methods Three different computational techniques are designed to examine the numerical solution of SIR model of infectious disease. Two approaches are well-known existing computing methods named as forward Euler finite difference (FD) method and backward Euler operator splitting finite difference (OS-FD) method. The third approach is operator splitting nonstandard finite difference (OS-NSFD) method which is devised by using the NSFD rules. Results The proposed OS-NSFD technique retains efficiently the stability of equilibria as well as the positivity. Graphical behavior depicts that the existing computing methods can not get success to preserve the structure of the epidemic system of whooping cough dynamics. At the same time OS-NSFD computing method is proven to be reliable and suitable for the system of bio-medical engineering mathematically and graphically. Conclusion A reliable and novel computing technique is developed for the solution of two dimensional reaction diffusion problem. This technique preserves all the imperative characteristics of the model under study. Also the time efficiency of this method makes it easy to find the solution of physical system in two space dimension. The comparison with other techniques shows the efficacy and reliability of the designed technique.

5.
Nano Lett ; 20(4): 2296-2302, 2020 04 08.
Artigo em Inglês | MEDLINE | ID: mdl-32091908

RESUMO

Single-molecule techniques have become impactful in bioanalytical sciences, though the advantages for continuous biosensing are yet to be discovered. Here we present a multiplexed, continuous biosensing method, enabled by an analyte-sensitive, single-molecular nanoswitch with a particle as a reporter. The nanoswitch opens and closes under the influence of single target molecules. This reversible switching yields binary transitions between two highly reproducible states, enabling reliable quantification of the single-molecule kinetics. The multiplexing functionality is encoded per particle via the dissociation characteristics of the nanoswitch, while the target concentration is revealed by the association characteristics. We demonstrate by experiments and simulations the multiplexed, continuous monitoring of oligonucleotide targets, at picomolar concentrations in buffer and in filtered human blood plasma.

6.
Sci Rep ; 10(1): 2775, 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32066818

RESUMO

This work represents the nature of conduction mechanism in bismuth silicate (BiSiO) nanofibers as a function of temperature and frequency. Scanning electron micrographs and X-rays diffraction patterns exhibited the formation of cubic phases of Bi4(SiO4)3 and Bi12SiO20 nanofibers respectively with an average diameter of ~200 nm. Temperature dependent (300 K-400 K) electrical characterization of fibers was carried out in frequency range of ~20 Hz-2 MHz. The complex impedance analysis showed contribution from bulk and intergranular parts of nanofibers in conduction. Moreover, analysis of the Cole-Cole plot confirmed the space charge dependent behavior of BiSiO nanofibers. Two types of relaxation phenomena were observed through Modulus analysis. In ac conductivity curve, step like feature of plateau and dispersive regions were described by Maxwell-Wagner effect while the dc part obeyed the Arrhenius law. However, frequency dependent ac conductivity revealed the presence of conduction mechanism in diverse regions that was ascribed to large polaron tunneling model. Detailed analysis of complex Impedance and ac conductivity measurement showed negative temperature coefficient of resistance for the BiSiO nanofibers. Current-voltage (IV) characteristics represented ohmic conduction; followed by space charge limited current conduction at intermediate voltages. Results from both ac and dc measurements were in good agreement with each other.

7.
Chaos ; 29(10): 103101, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31675795

RESUMO

In this work, we investigate the numerical solution of the susceptible exposed infected and recovered measles epidemic model. We also evaluate the numerical stability and the bifurcation value of the transmission parameter from susceptibility to a disease of the proposed epidemic model. The proposed method is a chaos free finite difference scheme, which also preserves the positivity of the solution of the given epidemic model.


Assuntos
Epidemias , Sarampo/epidemiologia , Modelos Biológicos , Suscetibilidade a Doenças , Humanos , Dinâmica Populacional
8.
ACS Omega ; 4(1): 2030-2039, 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-31459454

RESUMO

Orthorhombic phase bismuth sulfide (Bi2S3) nanobelts were prepared via  liquid-solid phase reaction method. Bi2S3 nanobelts were observed to be preferentially oriented along the (101) plane. Direct band gap (2.95 eV) and characteristic wavelength (λmax = 342 nm) were extracted through UV-visible spectroscopy. Specific surface area (9.8 m2/g) and pore size (2.5-120 nm) were evaluated through Brunauer-Emmett-Teller (BET) analysis. Relative humidity (RH) sensing properties were studied in the range of 11-97% RH at ambient conditions. The response of the sensor increases linearly with increase in RH. Fast response time (8-10 s) and recovery time (15 s) were observed. Reproducible and large response was also observed between 11 and 97% RH. Small hysteresis (<5%) and long-term stability during 30 days were confirmed. As a function of frequency, capacitance, alternating current conductivity, and electrical complex modulus in the frequency range of 20-2 MHz were studied at 11-97% RH. The sensing mechanism was also studied.

9.
Heliyon ; 4(5): e00631, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29872764

RESUMO

This work is concerned with the numerical modeling of susceptible-latent-breakingout-quarantine-susceptible (SLBQRS) computer virus dynamics. The SLBQRS epidemic system is solved with three finite difference methods, one is proposed nonstandard finite difference (NSFD) method and the other two are well known forward Euler finite difference (FD) method and Runge-Kutta finite difference method of order 4 (RK-4). The proposed NSFD method preserves all the essential conditions of the continuous system while RK-4 method and forward Euler method fail to preserve some of its essential conditions like positivity, convergence to the true steady states of the continuous system. The convergence analysis of the proposed NSFD method is also performed. Bifurcation value of infection coefficient for the system is also find out.

10.
J Appl Microbiol ; 125(2): 457-467, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29729069

RESUMO

AIM: Thiss study was conducted to investigate the possible role of a compatible solute from radio-halophilic bacterium against desiccation and ultra-violet radiation-induced oxidative stress. METHODS AND RESULTS: Nine different radio-resistant bacteria were isolated from desert soil, where strain WMA-LM19 was chosen for detailed studies on the basis of its high tolerance to ultraviolet radiation among all these isolates. Here, 16S rRNA gene sequencing indicated the bacterium was closely related to Stenotrophomonas sp. (KT008383). A bacterial milking strategy was applied for extraction of intracellular compatible solutes in 70% (v/v) ethanol, which were purified by High Performance Liquid Chromatography (HPLC). The compound was characterized as ectoine by 1 H and 13 C Nuclear Magnetic Resonance (NMR), and Mass Spectrometry (MS). Ectoine inhibited oxidative damage to proteins and lipids in comparison to the standard ascorbic acid. It also demonstrated more efficient prevention (54·80%) against lysis to erythrocytes membrane by surface active agents than lecithin. Furthermore, a high level of ectoine-mediated protection of bovine serum albumin against ionizing radiation (1 500-2 000Jm-2 ) was observed, as indicated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. CONCLUSION: The results indicated that ectoine from Stenotrophomonas sp. WMA-LM19 can be used as a potential mitigator and radio-protective agent to overcome radiation- and salinity-mediated oxidative damages in extreme environment. SIGNIFICANCE AND IMPACT OF THE STUDY: Due to its anti-oxidant properties, ectoine from a radio-halophilic bacterium might be used in sunscreen formulation for protection against UV-induced oxidative stress.


Assuntos
Diamino Aminoácidos/farmacologia , Protetores contra Radiação/farmacologia , Stenotrophomonas/química , Raios Ultravioleta/efeitos adversos , Animais , Membrana Eritrocítica/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Microbiologia do Solo , Stenotrophomonas/genética
11.
Mol Psychiatry ; 23(4): 973-984, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-28397838

RESUMO

Approximately 1% of the global population is affected by intellectual disability (ID), and the majority receive no molecular diagnosis. Previous studies have indicated high levels of genetic heterogeneity, with estimates of more than 2500 autosomal ID genes, the majority of which are autosomal recessive (AR). Here, we combined microarray genotyping, homozygosity-by-descent (HBD) mapping, copy number variation (CNV) analysis, and whole exome sequencing (WES) to identify disease genes/mutations in 192 multiplex Pakistani and Iranian consanguineous families with non-syndromic ID. We identified definite or candidate mutations (or CNVs) in 51% of families in 72 different genes, including 26 not previously reported for ARID. The new ARID genes include nine with loss-of-function mutations (ABI2, MAPK8, MPDZ, PIDD1, SLAIN1, TBC1D23, TRAPPC6B, UBA7 and USP44), and missense mutations include the first reports of variants in BDNF or TET1 associated with ID. The genes identified also showed overlap with de novo gene sets for other neuropsychiatric disorders. Transcriptional studies showed prominent expression in the prenatal brain. The high yield of AR mutations for ID indicated that this approach has excellent clinical potential and should inform clinical diagnostics, including clinical whole exome and genome sequencing, for populations in which consanguinity is common. As with other AR disorders, the relevance will also apply to outbred populations.


Assuntos
Consanguinidade , Deficiência Intelectual/genética , Adulto , Mapeamento Cromossômico/métodos , Variações do Número de Cópias de DNA , Família , Feminino , Genes Recessivos , Heterogeneidade Genética , Homozigoto , Humanos , Deficiência Intelectual/metabolismo , Irã (Geográfico) , Mutação com Perda de Função , Masculino , Análise em Microsséries/métodos , Pessoa de Meia-Idade , Mutação , Paquistão , Linhagem , Sequenciamento Completo do Exoma/métodos
12.
Saudi J Anaesth ; 10(4): 453-455, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27833494

RESUMO

Sanjad-Sakati syndrome is an autosomal recessive genetic disorder first described in Saudi Arabia. Anesthetic management of these patients is challenging due to airway difficulties, electrolyte imbalance, growth and mental retardation, and seizures. The anesthetic management of the syndrome is described in this case report.

13.
Eur J Neurol ; 23(6): 1071-8, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27029589

RESUMO

BACKGROUND AND PURPOSE: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative condition for which there is no single diagnostic test or biomarker. The level of the creatine kinase (CK) enzyme in serum may be mild to moderately elevated in some patients with ALS, the precise cause of which and its behaviour with disease progression is unknown. The aim of this study was to examine the usefulness of monitoring CK serially during the ALS disease trajectory and to determine whether CK levels mirror disease progression. METHODS: This was a prospective observational cohort study, using the clinical database of the olesoxime (TRO19622) investigational medicinal product trial. RESULTS: The baseline CK was raised in 52% of the trial participants with the mean CK ± SD being 257 ± 239 U/l. The mean CK was significantly higher in male participants than in female participants (P < 0.001) and amongst participants with limb onset ALS compared to participants with bulbar onset ALS (P < 0.001). There was no significant difference in the CK levels between upper limb and lower limb onset disease (P = 0.746). The CK level co-related positively with serum creatinine and estimated lean body mass but there was no relationship between CK and muscle scores and limb function. A higher CKlog was associated with significantly better survival, even when adjusted for prognostic co-variants (P = 0.013). CONCLUSIONS: The serum CK level seems to be an independent prognostic factor for survival in ALS. The cellular mechanism of CK enzyme suggests that it may be upregulated to provide energy in the face of metabolic stress in ALS.


Assuntos
Esclerose Amiotrófica Lateral/sangue , Índice de Massa Corporal , Creatina Quinase/sangue , Creatinina/sangue , Biomarcadores/sangue , Progressão da Doença , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos
14.
Pak J Med Sci ; 32(1): 151-4, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27022365

RESUMO

OBJECTIVE: This article compares the study design and statistical methods used in 2005, 2010 and 2015 of Pakistan Journal of Medical Sciences (PJMS). METHODS: Only original articles of PJMS were considered for the analysis. The articles were carefully reviewed for statistical methods and designs, and then recorded accordingly. The frequency of each statistical method and research design was estimated and compared with previous years. RESULTS: A total of 429 articles were evaluated (n=74 in 2005, n=179 in 2010, n=176 in 2015) in which 171 (40%) were cross-sectional and 116 (27%) were prospective study designs. A verity of statistical methods were found in the analysis. The most frequent methods include: descriptive statistics (n=315, 73.4%), chi-square/Fisher's exact tests (n=205, 47.8%) and student t-test (n=186, 43.4%). There was a significant increase in the use of statistical methods over time period: t-test, chi-square/Fisher's exact test, logistic regression, epidemiological statistics, and non-parametric tests. CONCLUSION: This study shows that a diverse variety of statistical methods have been used in the research articles of PJMS and frequency improved from 2005 to 2015. However, descriptive statistics was the most frequent method of statistical analysis in the published articles while cross-sectional study design was common study design.

15.
J Appl Microbiol ; 119(4): 1035-45, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26258580

RESUMO

AIM: This work was conducted to produce, purify and characterize biosurfactants from Aspergillus flavus AF612 isolated from citrus fruit. METHODS AND RESULTS: Biosurfactant named 'Uzmaq' was isolated from A. flavus AF612. The chemical characterization of the biosurfactant was conducted. Biosurfactant Uzmaq produced by A. flavus, was composed of methoxy phenyl oxime glycosides. Two molecular forms of the biosurfactant, Uzmaq-A and Uzmaq-B were isolated. Biological properties (antifungal activity) were evaluated. The fractions of the biosurfactant were isolated and their surface properties were analysed. Uzmaq-A and Uzmaq-B had critical micelle concentration (CMC) around 170 and 80 mg l(-1) , and lowered surface tension of water up to 20 and 25 m Nm(-1) respectively. The biosurfactants were stable at pH 3-12 and temperature up to 80°C. Growth and biosurfactant production kinetics were also analysed. CONCLUSION: Novel biosurfactant Uzmaq was produced from A. flavus, which was composed of methoxy phenyl oxime glycosides. The surface activity of Uzmaq was better than the maximum values of synthetic chemical surfactants. The biosurfactant showed antifungal activity and self-assembling properties. SIGNIFICANCE AND IMPACT OF THE STUDY: Aspergillus flavus AF612 can be used for commercial production of Uzmaq that may be employed for controlled drug release applications and bioremediation.


Assuntos
Aspergillus flavus/metabolismo , Tensoativos/química , Tensoativos/metabolismo , Aspergillus flavus/química , Cinética , Micelas , Propriedades de Superfície , Tensão Superficial , Temperatura
16.
Case Rep Surg ; 2015: 379258, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26167326

RESUMO

We report a rare case of bilateral vocal cord palsy following total thyroidectomy with successful extubation within 12 hours. The patient is a 33-year-old lady who underwent uneventful total thyroidectomy for compressive symptoms. Thirty minutes after extubation, she developed stridor and the flexible laryngoscopy showed bilaterally adducted vocal cords. The patient, thus, was reintubated and after 12 hours she met the extubation parameters and so she was extubated successfully. The repeated flexible laryngoscopy showed normal vocal cords. A review of the literature revealed limited information on this transient condition.

17.
Ortop Traumatol Rehabil ; 17(5): 481-7, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26751748

RESUMO

BACKGROUND: Hips can suffer severe damage due to untreated developmental dysplasia, septic arthritis, tuberculosis, a neglected fracture of the neck of femur and neglected hip dislocation. The Ilizarov technique offers an effective treatment by providing a stable hip with abolished Trendelenburg lurch and equalizing limb length discrepancy through distal lengthening realignment osteotomy. MATERIAL AND METHODS: 20 patients with hip instabilities due to various etiologies were treated with the Ilizarov technique of pelvic support osteotomy and distal lengthening realignment osteotomy. There were 12 females and 8 males in the study group and the age range was 13 to 30 years. Average limb length discrepancy was 5.95 cms (range 4-8.5 cms).The pre-operative and post-operative range of motion and Harris hip score was collected and data analyzed by Student's paired t test. A p value of < 0.05 was taken to be statistically significant Results. The functional Harris hip score improved in all the patients at final follow-up. The mean Harris hip score was 56.95 (range 33-71) pre-operatively, which improved to 83.25 (range 73-85) at final follow up and was statistically significant (P-value < 0.05). The mean length achieved was 5.53 cms (4-8 cms). The mean external fixation time was 8.6 months and the mean healing index was 1.54 months /cm. CONCLUSIONS: 1. Ilizarov hip reconstruction is an excellent method of salvage in patients with unstable hips diverse etiologies especially in this part of the world, where patients demand unrestricted range of motion at hip, and in the younger age group, where other procedures do not offer a long term solution. 2. It provides an excellent functional outcome in hips of different etiologies. 3. However, for an excellent outcome, surgical expertise, patient compliance and meticulous follow-up are mandatory.


Assuntos
Alongamento Ósseo/métodos , Articulação do Quadril/cirurgia , Técnica de Ilizarov , Instabilidade Articular/fisiopatologia , Instabilidade Articular/cirurgia , Osteotomia/métodos , Amplitude de Movimento Articular/fisiologia , Adolescente , Adulto , Feminino , Humanos , Masculino , Resultado do Tratamento , Adulto Jovem
19.
Biomed Res Int ; 2014: 975946, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24955374

RESUMO

Chromium (Cr) is present in our environment as a toxic pollutant, which needs to be removed using phytoremediation technology. In present study, two transgenic cotton cultivars (J208, Z905) and their hybrid line (ZD14) were used to explore their Cr uptake and tolerance potential using multiple biomarkers approach. Four different levels of Cr (CK, 10, 50, and 100 µM) were applied. Cr caused a significant reduction in root/shoot length, number of secondary roots, and root fresh and dry biomasses at 100 µM. Cr accumulated more in roots and was found higher in hybrid line (ZD14) as compared with its parent lines (J208, Z905) at all Cr stress levels (10, 50, and 100 µM). Cr translocation was less than 1 in all cultivars. Ultrastructural studies at 100 µM Cr showed an increase in number of nuclei and vacuoles and presence of Cr dense granules in dead parts of the cell (vacuoles/cell wall). Malondialdehyde (MDA), hydrogen peroxide (H2O2), total soluble proteins, superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR) as a whole were upregulated with elevated levels of Cr. Higher Cr uptake by roots, accelerated metabolism, and Cr sequestration in dead parts of the cell indicate that these cotton cultivars can be useful for Cr accumulation and tolerance.


Assuntos
Cromo/toxicidade , Gossypium/efeitos dos fármacos , Raízes de Plantas/efeitos dos fármacos , Plantas Geneticamente Modificadas/efeitos dos fármacos , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/ultraestrutura , Gossypium/crescimento & desenvolvimento , Estresse Oxidativo/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Poluentes do Solo/toxicidade , Vacúolos/efeitos dos fármacos , Vacúolos/ultraestrutura
20.
Biomed Res Int ; 2014: 839538, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24695876

RESUMO

Cadmium (Cd) is a widespread environmental toxic contaminant, which causes serious health-related problems. In this study, human intestinal cell line (Caco-2 cells) and normal human liver cell line (HL-7702 cells) were used to investigate the toxicity and bioavailability of Cd to both cell lines and to validate these cell lines as in vitro models for studying Cd accumulation and toxicity in human intestine and liver. Results showed that Cd uptake by both cell lines increased in a dose-dependent manner and its uptake by Caco-2 cells (720.15 µg mg(-1) cell protein) was significantly higher than HL-7702 cells (229.01 µg mg(-1) cell protein) at 10 mg L(-1). A time- and dose-dependent effect of Cd on cytotoxicity assays (LDH release, MTT assay) was observed in both Cd-treated cell lines. The activities of antioxidant enzymes and differentiation markers (SOD, GPX, and AKP) of the HL-7702 cells were higher than those of Caco-2 cells, although both of them decreased significantly with raising Cd levels. The results from the present study indicate that Cd above a certain level inhibits cellular antioxidant activities and HL-7702 cells are more sensitive to Cd exposure than Caco-2 cells. However, Cd concentrations <0.5 mg L(-1) pose no toxic effects on both cell lines.


Assuntos
Cádmio/metabolismo , Cádmio/toxicidade , Antioxidantes/metabolismo , Disponibilidade Biológica , Biomarcadores/metabolismo , Células CACO-2 , Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Humanos , L-Lactato Desidrogenase/metabolismo , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...