Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 65
Filtrar
1.
Arch. Soc. Esp. Oftalmol ; 95(12): 586-590, dic. 2020. tab
Artigo em Espanhol | IBECS | ID: ibc-194908

RESUMO

OBJETIVO: Reportar una experiencia piloto de atención por telemedicina en la especialidad de oftalmología, en el periodo de confinamiento por la pandemia por COVID-19. MÉTODOS: Estudio descriptivo. Se describen características demográficas y clínicas de pacientes atendidos en periodo de confinamiento de 10 semanas. Se evalúa la satisfacción de los pacientes y médicos participantes mediante una encuesta en línea. RESULTADOS: En las primeras 10 semanas, se realizaron 291 atenciones de telemedicina oftalmológica. Los principales motivos de consulta fueron afecciones inflamatorias de la superficie ocular y párpados (79,4%), seguido de requerimientos administrativos (6,5%), afecciones no inflamatorias de la superficie ocular (5,2%), sospecha de estrabismo (3,4%) y síntomas vitreorretinales (3,1%); 22 pacientes (7,5%) fueron derivados a atención presencial inmediata. El nivel de satisfacción con la prestación fue alto, tanto en médicos (100%), como en pacientes (93,4%). CONCLUSIONES: La atención oftalmológica por telemedicina en periodo de pandemia es un instrumento de utilidad para realizar un filtro de potenciales consultas presenciales, ya sea electivas o de urgencia, y para reducir potencialmente el riesgo de contagio por COVID-1


BACKGROUND: To report a pilot experience of telemedicine in ophthalmology in open-care modality (i.e. direct video call), in a confinement period due to the COVID-19 pandemic. METHODS: Descriptive study of the demographic and clinical characteristics of patients attended in a 10-week confinement period. Reported satisfaction of the participating patients and doctors was evaluated through an online survey. RESULTS: In the 10-week period, 291 ophthalmologic telemedicine consultations were performed. The main reasons for consultation were inflammatory conditions of the ocular surface and eyelids (79.4%), followed by administrative requirements (6.5%), non-inflammatory conditions of the ocular surface (5.2%), strabismus suspicion (3.4%) and vitreo-retinal symptoms (3.1%). According to previously defined criteria, 22 patients (7.5%) were referred to immediate face-to-face consultation. The level of satisfaction was high, both in doctors (100%) and in patients (93.4%). CONCLUSIONS: Open-care modality of telemedicine in ophthalmology during the pandemic period is a useful instrument to filter potential face-to-face consultations, either elective or emergency, and potentially reduce the risk of COVID-19 infection


Assuntos
Humanos , Feminino , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Oftalmopatias/diagnóstico , Oftalmopatias/terapia , Telemedicina/instrumentação , Projetos Piloto , Pandemias/prevenção & controle , Infecções por Coronavirus/prevenção & controle , Pneumonia Viral/prevenção & controle , Teleoftalmologia , Betacoronavirus , Satisfação do Paciente , Garantia da Qualidade dos Cuidados de Saúde/métodos , Garantia da Qualidade dos Cuidados de Saúde/estatística & dados numéricos
2.
Arch Soc Esp Oftalmol ; 95(12): 586-590, 2020 Dec.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-33160746

RESUMO

BACKGROUND: To report a pilot experience of telemedicine in ophthalmology in open-care modality (i.e. direct video call), in a confinement period due to the COVID-19 pandemic. METHODS: Descriptive study of the demographic and clinical characteristics of patients attended in a 10-week confinement period. Reported satisfaction of the participating patients and doctors was evaluated through an online survey. RESULTS: In the 10-week period, 291 ophthalmologic telemedicine consultations were performed. The main reasons for consultation were inflammatory conditions of the ocular surface and eyelids (79.4%), followed by administrative requirements (6.5%), non-inflammatory conditions of the ocular surface (5.2%), strabismus suspicion (3.4%) and vitreo-retinal symptoms (3.1%). According to previously defined criteria, 22 patients (7.5%) were referred to immediate face-to-face consultation. The level of satisfaction was high, both in doctors (100%) and in patients (93.4%). CONCLUSIONS: Open-care modality of telemedicine in ophthalmology during the pandemic period is a useful instrument to filter potential face-to-face consultations, either elective or emergency, and potentially reduce the risk of COVID-19 infection.

3.
Neoplasma ; 67(2): 410-414, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31884802

RESUMO

Keratin-15 (KRT15) is a type I keratin lacking a defined type II partner and plays a key role in maintaining cytoplasmic stability. Recently, studies have reported that KRT15 was correlated with tumor formation and progression. However, the clinical significance of KRT15 in colorectal cancer is unclear. In this study, we aimed to investigate the expression of KRT15 and its clinical significance in colorectal cancer. KRT15 expression was examined in 98 cases of colorectal cancer and matched adjacent normal tissues by quantificational real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), respectively. Then, the clinical significance of KRT15 expression was evaluated in colorectal cancer. QRT-PCR results revealed that the mRNA levels of KRT15 in colorectal cancer tissues were significantly higher compared with those in normal tissues (p<0.0001). The rates of KRT15 high-expression in colorectal cancer and normal tissues were 57.1% and 8.9%, respectively, and the difference was statistically significant (p<0.0001). KRT15 high-expression correlated with differentiation, T stage, lymph node metastasis and clinical stage in colorectal cancer (p<0.05). Meanwhile, KRT15 overexpression predicted poor prognosis and could be used as an independent prognostic factor. These data indicate KRT15 is highly expressed in colorectal cancer and may serve as a prognostic biomarker.


Assuntos
Neoplasias Colorretais/diagnóstico , Queratina-15/genética , Biomarcadores Tumorais/genética , Neoplasias Colorretais/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Metástase Linfática , Estadiamento de Neoplasias , Prognóstico , Reação em Cadeia da Polimerase em Tempo Real
4.
Zhonghua Nei Ke Za Zhi ; 58(2): 125-132, 2019 Feb 01.
Artigo em Chinês | MEDLINE | ID: mdl-30704199

RESUMO

Objective: To explore the role of lung dendritic cells (DCs) and Th17/regulatory T cells (Treg) pathway in the pathogenesis of chronic obstructive pulmonary disease(COPD). Methods: COPD patients who received lobectomy from Sep. 2015 to Mar. 2016 in our hospital were enrolled and classified into non-smoking non-COPD group, smoking without COPD group and COPD group. The expression of CD(80), chemokine recepter-6 (CCR6), interleukin-17A (IL-17A) and fork-head transcription factor P3 (FoxP3) were detected by immunohistochemistry (IHC) in lung tissue. Mature DCs (mDCs), immature DCs (imDCs), Th17 cells and Treg cells in lung tissue were detected by flow cytometry (FCM) and the correlation between Th17/Treg cells with lung function was analyzed. Results: (1) The expression of CD(80) and FoxP3 in COPD group was decreased, while the expression of CCR6 and IL-17A was increased (P<0.05). (2) The percentage of mDCs and Treg in lung tissue of COPD group was significantly decreased. In contrast, the proportion of imDCs and Th17 cells in COPD group was significantly increased (P<0.05). (3) The imbalance of Th17/Treg ratio in lung tissue was seen in patients with COPD, suggesting the potential mechanism of Th17 cell-mediated proinflammatory response. (4) The percentage of Th17 cells and Th17/Treg ratio in COPD patients was negatively correlated with forced expiratory volume in the first second (FEV(1)) as a percentage of predicted value (FEV(1)% pred), forced vital capacity(FVC) as a percentage of predicted value (FVC% pred), FEV(1)/FVC. On the other hand, the percentage of Treg cells was positively correlated with FEV(1)% pred, FVC% pred, FEV(1)/FVC. Conclusions: The data in this study demonstrate the maturation disorder of dendritic cells in lung tissue of COPD patients. The imbalance of Th17/Treg ratio suggests that Th17 cell-mediated proinflammatory response may be involved in the pathogenesis of COPD.


Assuntos
Células Dendríticas/fisiologia , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Transdução de Sinais , Linfócitos T Reguladores/fisiologia , Células Th17/fisiologia , Humanos , Pulmão/citologia , Doença Pulmonar Obstrutiva Crônica/terapia
5.
Beijing Da Xue Xue Bao Yi Xue Ban ; 50(6): 1098-1101, 2018 Dec 18.
Artigo em Chinês | MEDLINE | ID: mdl-30562789

RESUMO

Aggressive angiomyxoma is a rare mesenchymal tumor. To discuss the clinicopathological characteristics, treatment and prognosis of aggressive angiomyxoma, four cases of aggressive angiomyxoma of soft tissue in abdominopelvic cavity were collected from January 2015 to August 2017 in Peking University International Hospital. The clinical data, imaging examination, histopathological features, immunophenotype, therapy and prognosis were analysed. The related literatures were reviewed. All of the patients were adult females, age range from 27 to 49 years and mean 33 years. The clinical complaint was abdominal distention with no definite predisposing factor, or occasional physical-exam finding with no obvious discomfort. Three cases were primary and one case was recurrent. Typical layered or swirled structural sign was presented by CT and MRI scanning of three cases. All tumors located in the pelvic cavity, and attached to the uterus, vagina, rectum, bladder or ureter. One case was involved in the abdominal cavity simultaneously,adhesive to the spine, inferior vena cava and spleen. The gross appearance of tumors was from 5 to 22 cm in maximum diameter. The sectioned surfaces were soft, solid, white or yellow-gray, focally accompanied by edema, mucoid degeneration or cystic change. Microscopic observation showed that tumor cells were short spindle shaped and little atypical, the stroma was loose like edematous mucus or collagen, and the vessels were rich in thin and thick-wall. Partially the vessel wall expressed hyaline degeneration. Also tumors might infiltrate surrounding tissue, such as fat or nerve. The immunohistochemistry results of all cases were estrogen receptor and progesterone receptor diffusely moderate positive, Desmin and smooth muscle actin mostly positive, whereas CD34 expressed only in vessel and S-100 protein, CD117 and Dog1 all negative. All the tumors were complete surgical excision. During follow-up, one case recurred the second time. Our conclusions are the diagnosis of aggressive angiomyxoma is based on pathological morphology supplemented by immunohistochemistry, and the tumor may relapse after surgical resection.


Assuntos
Mixoma , Receptores Estrogênicos , Adulto , Desmina/análise , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Mixoma/diagnóstico , Mixoma/patologia , Recidiva Local de Neoplasia , Receptores Estrogênicos/análise
6.
Colorectal Dis ; 20(6): O135-O142, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29577541

RESUMO

AIM: This study aimed to analyse the potential significance of metastasis to the inferior mesenteric artery lymph node (IMA-LN) in patients with malignancy of the left colon and rectum. METHOD: A retrospective analysis of a cohort of 890 patients collected prospectively who underwent radical resection of a primary tumour of the descending colon, sigmoid colon and rectum in our department from 1 January 2009 to 31 December 2015 was performed. Patients were divided into an IMA-LN metastasis (IMA-LN (+)) group (n = 51) and a non IMA-LN metastasis (IMA-LN (-)) group (n = 839). A total of 801 patients were followed by a designated member of the study staff. Clinical features, pathological characteristics, recurrence patterns and survival rates were compared between the two groups. RESULTS: In the IMA-LN (+) group, the risk ratio of overall recurrence and tumour related death was 7.786 (95% CI 4.142-14.637) and 7.756 (95% CI 4.142-14.525) respectively. Significant differences were found in overall survival (log-rank: χ2  = 69.06, P < 0.0001) and disease-free survival (log-rank: χ2  = 69.06, P < 0.0001) between the two groups. Furthermore, there were significant differences in overall survival (log-rank: χ2  = 18.47, P < 0.0001) and disease-free survival (log-rank: χ2  = 16.99, P < 0.0001) between the IMA-LN (-) and IMA-LN (+) subgroups of patients with Stage N2 disease. Multivariate survival analysis indicated that IMA-LN (+) was an independent risk factor of poor prognosis. There was no difference in the prognosis between high tie and low tie with IMA-LN dissection. CONCLUSION: Inferior mesenteric artery lymph node metastasis was an independent predictive factor for high systemic recurrence. Low ligation of the IMA with IMA-LN dissection was not inferior to high ligation.


Assuntos
Adenocarcinoma/patologia , Neoplasias Colorretais/patologia , Linfonodos/patologia , Adenocarcinoma/mortalidade , Adenocarcinoma/cirurgia , Adenocarcinoma Mucinoso/mortalidade , Adenocarcinoma Mucinoso/patologia , Adenocarcinoma Mucinoso/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Colo Descendente/patologia , Colo Descendente/cirurgia , Colo Sigmoide/patologia , Colo Sigmoide/cirurgia , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/cirurgia , Intervalo Livre de Doença , Feminino , Humanos , Estimativa de Kaplan-Meier , Excisão de Linfonodo , Metástase Linfática , Masculino , Artéria Mesentérica Inferior , Mesentério , Pessoa de Meia-Idade , Análise Multivariada , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Análise de Sobrevida , Taxa de Sobrevida
7.
Zhonghua Bing Li Xue Za Zhi ; 47(2): 94-98, 2018 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-29429159

RESUMO

Objective: To investigate the clinicopathological features, differential diagnosis, treatment and prognosis of dedifferentiated liposarcoma with rhabdomyoblastic differentiation. Methods: Six cases of retroperitoneal dedifferentiated liposarcoma with rhabdomyoblastic features were collected from December 2014 to August 2017 at Peking University International Hospital. The clinical manifestations, histomorphology, immunophenotype, treatment and follow-up data were analyzed, and relevant literature reviewed. Results: The six patients included two males and four females, with age range of 47 to 66 years (mean 56 years). One case was primary and the five cases were recurred; four cases received radiotherapy and/or chemotherapy. The tumor diameters were 10 to 30 cm. Microscopically, the dedifferentiated areas were well demarcated from the well-differentiated areas, and resembled malignant fibrous histiocytoma, fibrosarcoma or solitary fibrous tumor with obvious mitotic figures or necrosis. Rhabdomyoblastic cells made up 10% to 30% of dedifferentiated area, and were scattered or focally distributed, being rounded, band-like or spindled, mostly with abundant eosinophilic cytoplasm. No striated structure was found, and the nucleis were rounded, oval or irregular shape with central or eccentric prominent nucleoli. Rare rhabdomyoblastic cells were lymphocytoid. The tumors encroached the muscular layer of intestinal wall in two cases and perirenal adipose tissue in one case. By immunohistochemical staining, the rhabdomyoblastic cells of all cases were all positive for desmin, myogenin, myoD1 and SMA; S-100 protein was expressed in one case (1/6). Well-differentiated area in two cases and dedifferentiated areas in all six cases were positive for MDM2, CDK4 and p16. After resection of the tumor and adjacent organs, one case recurred three months later, but there was no distant metastasis. Conclusions: Dedifferentiated liposarcoma with rhabdomyoblastic differentiation is a rare dedifferentiated liposarcoma. Pathological diagnosis is based on morphology, with supplementary immunohistochemical or molecular evaluation for further differential diagnosis. Multiple relapses may occur after surgical ablation plus adjuvant therapy.


Assuntos
Lipossarcoma/patologia , Neoplasias Retroperitoneais/patologia , Idoso , Contagem de Células , Diferenciação Celular , Diagnóstico Diferencial , Feminino , Fibrossarcoma/patologia , Histiocitoma Fibroso Maligno/patologia , Humanos , Imunofenotipagem , Lipossarcoma/terapia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Prognóstico , Neoplasias Retroperitoneais/terapia , Tumores Fibrosos Solitários/patologia
8.
J Viral Hepat ; 25(7): 842-852, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29406564

RESUMO

The relation between hepatitis B virus (HBV) infection and fatty liver has been addressed by several observational studies, but their results remain controversial. To date, no study has precisely investigated the association of current and past HBV infection with the risk of nonalcoholic fatty liver disease (NAFLD) in the Chinese population. Therefore, we conducted a hospital-based case-control study in southwestern China to clarify this issue. A total of 631 newly ultrasound-diagnosed NAFLD cases and 2357 controls were selected from 123 243 consecutive patients admitted to a tertiary-care hospital between January 2015 and December 2016. Multivariate logistic regression was employed to calculate adjusted odds ratios (ORs) and 95% confidence intervals (CIs). A propensity score was developed for adjustment and matching. Subgroup analysis was conducted to identify potential effect modifiers. Current and past HBV infection had an overall prevalence of 9.7% and 55.2%, respectively. In the fully adjusted model, current HBV infection was associated with a decreased risk of NAFLD (OR 0.64; 95% CI 0.42-0.95). A similar inverse association was observed in both propensity-score-adjusted (OR 0.58; 95% CI 0.40-0.86) and propensity-score-matched analyses (OR 0.61; 95% CI 0.40-0.92).The inverse association was stronger in patients with hypertension than in those without (Pinteraction  = .018).No significant association between past HBV infection and NAFLD risk was found. In conclusion, current but not past HBV infection is associated with a decreased risk of NAFLD in the Chinese population. The corresponding biological mechanisms remain to be elucidated.


Assuntos
Hepatite B/complicações , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Idoso , Estudos de Casos e Controles , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/diagnóstico por imagem , Prevalência , Pontuação de Propensão , Medição de Risco , Ultrassonografia
9.
Insect Mol Biol ; 26(6): 665-676, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-28703893

RESUMO

Peptidoglycan is the major bacterial component recognized by the insect immune system. Peptidoglycan recognition proteins (PGRPs) are a family of pattern-recognition receptors that recognize peptidoglycans and modulate innate immune responses. Some PGRPs retain N-acetylmuramoyl-L-alanine amidase (Enzyme Commission number: 3.5.1.28) activity to hydrolyse bacterial peptidoglycans. Others have lost the enzymatic activity and work only as immune receptors. They are all important modulators for innate immunity. Here, we report the cloning and functional analysis of PGRP-S4, a short-form PGRP from the domesticated silkworm, Bombyx mori. The PGRP-S4 gene encodes a protein of 199 amino acids with a signal peptide and a PGRP domain. PGRP-S4 was expressed in the fat body, haemocytes and midgut. Its expression level was significantly induced by bacterial challenges in the midgut. The recombinant PGRP-S4 bound bacteria and different peptidoglycans. In addition, it inhibited bacterial growth and hydrolysed an Escherichia coli peptidoglycan in the presence of Zn2+ . Scanning electron microscopy showed that PGRP-S4 disrupted the bacterial cell surface. PGRP-S4 further increased prophenoloxidase activation caused by peptidoglycans. Taken together, our data suggest that B. mori PGRP-S4 has multiple functions in immunity.


Assuntos
Bombyx/imunologia , Proteínas de Transporte/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Bombyx/genética , Bombyx/metabolismo , Proteínas de Transporte/isolamento & purificação , Proteínas de Transporte/metabolismo , Catecol Oxidase/metabolismo , Precursores Enzimáticos/metabolismo , Proteínas de Insetos/genética , Proteínas de Insetos/isolamento & purificação , Proteínas de Insetos/metabolismo , Larva/metabolismo , Peptidoglicano/metabolismo , Análise de Sequência de DNA
10.
Artigo em Chinês | MEDLINE | ID: mdl-27480302

RESUMO

OBJECTIVE: To evaluate the practice of peripheral blood eosinophil in the diagnosis of eosinophilic chronic rhinosinusitis(ECRS). METHODS: The correlation between eosinophil count and percentage in peripheral blood and that in topic tissue in 787 patients (the first affiliated hospital of Wenzhou medical university, from Jan. 2013 to Jun. 2016) with chronic rhinosinusitis (CRS) were retrospectively analysed. The optimal cutoff value of blood eosinophil count and percentage as predictors for ECRS was determined by receiver operating characteristic curves (ROC) and their diagnostic ability was compared. RESULTS: The positive correlation between eosinophil count and percentage in blood and that in tissue was found respectively in 787 patients with CRS (r=0.450, 0.499, 0.463, 0.465, P<0.01). Although the significant correlation between blood eosinophil count and its count and percentage in tissue was not found after blood leukocyte and tissue eosinophilic inflammation was controlled respectively (r=0.041, P=0.380; r=0.046, P=0.329 and r=0.023, P=0.618; r=0.032, P=0.499), blood eosinophil percentage still showed significant correlation with tissue eosinophil count and percentage, but reduced unequally after that(r=0.383, 0.436 and r=0.153, 0.169, P<0.01). With ROC analysis, the diagnostic ability of optimal cut off values of eosinophil count and percentage varied as the histological criteria for ECRS differed. CONCLUSIONS: Eosinophil in peripheral blood showed significant positive correlation with its tissue infiltration, which may be not strong and easily effected by individual factors.Theoretically, blood eosinophil may have a diagnostic significance as a predictor for ECRS but not practically.


Assuntos
Eosinófilos , Rinite/diagnóstico , Sinusite/diagnóstico , Doença Crônica , Humanos , Contagem de Leucócitos , Curva ROC , Estudos Retrospectivos , Rinite/sangue , Sinusite/sangue
11.
Genet Mol Res ; 15(2)2016 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-27420975

RESUMO

A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of auraptene, a constituent isolated from Fructus aurantii with potential to combat Alzheimer's disease, in rat plasma. Rat plasma samples were pretreated by protein precipitation with methanol. The analytes were separated by a Waters Sun Fire C18 column (50 mm x 2 mm, 5 µm) and eluted with 1:1000 methanol and formic acid/water (v/v) mobile phase with a flow rate of 0.5 mL/min. Multiple reaction monitoring was used to monitor the transition of the deprotonated auraptene molecule with an m/z of 299.3 [M+H](+), to the product ion with an m/z of 162.9 [M+H](+). Progesterone, with an m/z of 315.2→ 96.9 was used as an internal standard. The limits of detection and of quantification of auraptene in the rat plasma were 1 and 5 ng/mL, respectively. The method was linear in the concentration range of 20- 2000 ng/mL with coefficient correlation of 0.9956. After auraptene (100 mg/kg, p.o.) administration, the maximum plasma concentration and the time taken to reach maximum concentration were 1719.5 ± 384.3 g/mL and 108.0 ± 25.3 min, respectively. The elimination half-life was 108.0 ± 25.3 for auraptene (100 mg/kg, p.o.) and 3.0 ± 0 min for auraptene (2 mg/kg, i.v.). The oral bioavailability was about 8.5%.


Assuntos
Análise Química do Sangue/métodos , Cumarínicos/sangue , Espectrometria de Massas/métodos , Animais , Disponibilidade Biológica , Análise Química do Sangue/normas , Cromatografia Líquida/métodos , Cromatografia Líquida/normas , Cumarínicos/farmacocinética , Masculino , Espectrometria de Massas/normas , Ratos , Ratos Sprague-Dawley , Sensibilidade e Especificidade
12.
Genet Mol Res ; 15(2)2016 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-27173242

RESUMO

Published studies on the association between the C677T and A1298C polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene and male infertility risk are controversial. To obtain a more precise evaluation, we performed a meta-analysis based on published case-control studies. We conducted an electronic search of PubMed, EMBASE, the Cochrane Library, the Web of Science, and the China Knowledge Resource Integrated Database for papers on MTHFR gene C677T and A1298C polymorphisms and male infertility risk. Pooled odds ratios (ORs) with 95% confidence intervals (95%CIs) were used to assess the strength of association in homozygote, heterozygote, dominant, recessive, and additive models. Statistical heterogeneity, test of publication bias, and sensitivity analysis were carried out using the STATA software (Version 13.0). Overall, 21 studies of C677T (4505 cases and 4024 controls) and 13 studies of A1298C (2785 cases and 3094 controls) were included in this meta-analysis. For C677T, the homozygote comparison results were OR = 1.629, 95%CI (1.215- 2.184), and the recessive model results were OR = 1.462 (1.155- 1.850). For A1298C, the homozygote comparison results were OR = 1.289 (1.029-1.616), and the recessive model results were OR = 1.288 (1.034-1.604). In conclusion, the current meta-analysis showed that the MTHFR C677T polymorphism was associated with a significantly increased male infertility risk in the Asian and overall populations, but not in the Caucasian population, and there was a significant association between the A1298C polymorphism and male infertility risk in the Asian, Caucasian, and overall groups.


Assuntos
Infertilidade/genética , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Polimorfismo de Nucleotídeo Único , Grupo com Ancestrais do Continente Asiático , Estudos de Casos e Controles , Grupo com Ancestrais do Continente Europeu , Humanos , Infertilidade/etnologia , Masculino
13.
Mater Sci Eng C Mater Biol Appl ; 66: 297-305, 2016 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-27207066

RESUMO

Plasma electrolytic oxidation (PEO) was employed to grow different porous titania structures on Ti6Al4V alloy (TC4) substrate using various parameters. It was found that the PEO voltage and working frequency could affect the morphology, the pore size, the pore density, the thickness and the phase composition of titania structures. Thereafter, three typical porous titania structures with nanosize pores, microsize pores and microsize grooves were respectively selected to estimate their bioactivity using SBF immersion test. After soaking at different durations (3-28d), the surface morphology, the chemical composition as well as the phase structure of deposited apatite layers on porous titania were evaluated using SEM, EDS, and XRD. The formation of various biomimetic apatite layers indicated the different influence due to the characteristics of porous titania structures. The porous titania structure with nanosize pores could induce a fast apatite growth at the early immersion stage (~7d), while the one with microsize pores exhibited the best apatite inducing ability at long term immersion (~28d). Based on the experimental results, the formation mechanism of biomimetic apatite affected by the pore structure of titania was discussed as well.


Assuntos
Apatitas/química , Titânio/química , Microscopia Eletrônica de Varredura , Oxirredução , Gases em Plasma , Porosidade , Espectrometria por Raios X , Difração de Raios X
14.
Eur J Gynaecol Oncol ; 37(1): 30-5, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27048106

RESUMO

PURPOSE: Plumbagin, a naphthoquinone constituent of Plumbago zeylanica L. (Plumbaginaceae), is known to exhibit proapoptotic, antiangiogenic and antimetastatic effects in cancer cells. However, the effect of Plumbagin on breast cancer cells and the underlying molecular mechanism has not yet been elucidated. MATERIALS AND METHODS: MCF-7 (a human breast cancer cell line) was exposed different concentrations of Plumbagin (PG), and the anti-proliferative activity was evaluated by the MTT assay. The mechanism of action for the growth inhibitory activity of Plumbagin on MCF-7 cancer cells was evaluated using flow cytometry for cell cycle distribution, and western blot for assessment of expression of potential target proteins. RESULTS: Plumbagin exhibited a significant anti-proliferative activity against human breast cancer cells. Flow cytometric analysis revealed that Plumbagin caused cell cycle arrest at G1 phase. The cell cycle arrest was well correlated with the inhibition of cyclin D1, cyclin E, and upregulation of tumor suppressor protein p53. It further inhibited the expression of anti-apoptotic Bcl-2 family members such as Bcl-xL and Bcl-2, and activated pro-apoptotic proteins like Bax and Bak. CONCLUSION: These findings suggest that the anti-proliferative effect of Plumbagin is due to upregulation of p53 and p21 and suppression of G1 cell cycle regulators.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Inibidor de Quinase Dependente de Ciclina p21/genética , Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos , Naftoquinonas/farmacologia , Proteína Supressora de Tumor p53/genética , Proliferação de Células/efeitos dos fármacos , Humanos , Células MCF-7 , Proteínas Proto-Oncogênicas c-bcl-2/análise , Regulação para Cima
15.
Acta Virol ; 58(2): 167-72, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24957722

RESUMO

Watermelon silver mottle virus (WSMoV) is an emerging disease of cucurbit crops in South China. Production of high-quality antibodies is necessary for the development of serological methods for detection of this virus. The nucleocapsid protein (NP) gene of WSMoV was amplified from WSMoV-infected watermelon leaves by RT-PCR and cloned into vector pET-28a (+) for prokaryotic expression. After identification via enzyme digestion and sequencing, the recombinant clone was transformed into Escherichia coli Rosetta (DE3) for protein expression. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) results showed that the molecular weight of the WSMoV NP fusion protein was 34.1 kDa. The fusion protein was purified and used as antigen for the preparation of polyclonal antisera in rabbits. Results of indirect ELISA and western blot analysis showed that the antisera reacted specifically with WSMoV NP. In addition, sensitivity and specificity of the antisera were examined on a number of infected field samples by indirect ELISA. These findings will facilitate further immunological and serological studies of WSMoV. .


Assuntos
Anticorpos Antivirais/análise , Anticorpos/análise , Proteínas do Nucleocapsídeo/imunologia , Doenças das Plantas/virologia , Tospovirus/isolamento & purificação , Animais , Anticorpos/imunologia , Anticorpos Antivirais/imunologia , Western Blotting , Citrullus/virologia , Proteínas do Nucleocapsídeo/análise , Proteínas do Nucleocapsídeo/genética , Coelhos , Tospovirus/imunologia
16.
J Mech Behav Biomed Mater ; 34: 27-36, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24556322

RESUMO

Porous Ti-Nb-Zr alloys with different porosities from 6.06 to 62.8% are prepared by a two-step foaming powder metallurgy method using TiH2, Nb, and Zr powders together with 0 to 50wt% of NH4HCO3. The effects of the amounts of Nb and Zr as well as the sintering temperature (1473 to 1673K) on their phase composition, porosity, morphology, and mechanical characteristics are investigated. By controlling the porosity, Nb and Zr concentrations as well as the sintering temperature, porous Ti-Nb-Zr alloys with different mechanical properties can be obtained, for example, the hardness between 290 and 63HV, the compressive strength between 1530.5 and 73.4MPa, and the elastic modulus between 10.8 and 1.2GPa. The mechanical properties of the sintered porous Ti-Nb-Zr alloys can be tailored to match different requirements for the human bones and are thus potentially useful in the hard tissue implants.


Assuntos
Ligas/química , Teste de Materiais , Fenômenos Mecânicos , Metalurgia , Materiais Biocompatíveis/química , Módulo de Elasticidade , Dureza , Humanos , Nióbio/química , Porosidade , Pós , Propriedades de Superfície , Titânio/química , Zircônio/química
17.
Oncogene ; 32(38): 4519-28, 2013 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-23128390

RESUMO

Caveolin-1 (Cav1) is an integral membrane, scaffolding protein found in plasma membrane invaginations (caveolae). Cav1 regulates multiple cancer-associated processes. In breast cancer, a tumor suppressive role for Cav1 has been suggested; however, Cav1 is frequently overexpressed in aggressive breast cancer subtypes, suggesting an oncogenic function in advanced-stage disease. To further delineate Cav1 function in breast cancer progression, we evaluated its expression levels among a panel of cell lines representing a spectrum of breast cancer phenotypes. In basal-like (the most aggressive BC subtype) breast cancer cells, Cav1 was consistently upregulated, and positively correlated with increased cell proliferation, anchorage-independent growth, and migration and invasion. To identify mechanisms of Cav1 gene regulation, we compared DNA methylation levels within promoter 'CpG islands' (CGIs) with 'CGI shores', recently described regions that flank CGIs with less CG-density. Integration of genome-wide DNA methylation profiles ('methylomes') with Cav1 expression in 30 breast cancer cell lines showed that differential methylation of CGI shores, but not CGIs, significantly regulated Cav1 expression. In breast cancer cell lines having low Cav1 expression (despite promoter CGI hypomethylation), we found that treatment with a DNA methyltransferase inhibitor induced Cav1 expression via CGI shore demethylation. In addition, further methylome assessments revealed that breast cancer aggressiveness associated with Cav1 CGI shore methylation levels, with shore hypermethylation in minimally aggressive, luminal breast cancer cells and shore hypomethylation in highly aggressive, basal-like cells. Cav1 CGI shore methylation was also observed in human breast tumors, and overall survival rates of breast cancer patients lacking estrogen receptor α (ERα) negatively correlated with Cav1 expression. Based on this first study of Cav1 (a potential oncogene) CGI shore methylation, we suggest this phenomenon may represent a new prognostic marker for ERα-negative, basal-like breast cancer.


Assuntos
Neoplasias da Mama/genética , Caveolina 1/genética , Ilhas de CpG , Metilação de DNA , Regulação Neoplásica da Expressão Gênica , Azacitidina/farmacologia , Neoplasias da Mama/metabolismo , Neoplasias da Mama/mortalidade , Caveolina 1/metabolismo , Linhagem Celular Tumoral , Receptor alfa de Estrogênio/genética , Receptor alfa de Estrogênio/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Proteínas Oncogênicas/genética , Proteínas Oncogênicas/metabolismo , Regiões Promotoras Genéticas
18.
Oncogene ; 30(9): 1082-97, 2011 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-21057537

RESUMO

Fulvestrant is a selective estrogen receptor downregulator (SERD) and highly effective antagonist to hormone-sensitive breast cancers following failure of previous tamoxifen or aromatase inhibitor therapies. However, after prolonged fulvestrant therapy, acquired resistance eventually occurs in the majority of breast cancer patients, due to poorly understood mechanisms. To examine a possible role(s) of aberrantly expressed microRNAs (miRNAs) in acquired fulvestrant resistance, we compared antiestrogen-resistant and -sensitive breast cancer cells, revealing the overexpression of miR-221/222 in the SERD-resistant cell lines. Fulvestrant treatment of estradiol (E2)- and fulvestrant-sensitive MCF7 cells resulted in increased expression of endogenous miR-221/222. Ectopic upregulation of miR-221/222 in estrogen receptor-α (ERα)-positive cell lines counteracted the effects of E2 depletion or fulvestrant-induced cell death, thus also conferring hormone-independent growth and fulvestrant resistance. In cells with acquired resistance to fulvestrant, miR-221/222 expression was essential for cell growth and cell cycle progression. To identify possible miR-221/222 targets, miR-221- or miR-222- induced alterations in global gene expression profiles and target gene expression at distinct time points were determined, revealing that miR-221/222 overexpression resulted in deregulation of multiple oncogenic signaling pathways previously associated with drug resistance. Activation of ß-catenin by miR-221/222 contributed to estrogen-independent growth and fulvestrant resistance, whereas TGF-ß-mediated growth inhibition was repressed by the two miRNAs. This first in-depth investigation into the role of miR-221/222 in acquired fulvestrant resistance, a clinically important problem, demonstrates that these two 'oncomirs' may represent promising therapeutic targets for treating hormone-independent, SERD-resistant breast cancer.


Assuntos
Antineoplásicos/farmacologia , Neoplasias da Mama/genética , Resistencia a Medicamentos Antineoplásicos , Estradiol/análogos & derivados , MicroRNAs/genética , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Estradiol/farmacologia , Antagonistas de Estrogênios/farmacologia , Receptor alfa de Estrogênio/metabolismo , Feminino , Fulvestranto , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , MicroRNAs/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Moduladores Seletivos de Receptor Estrogênico/farmacologia , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Fator de Crescimento Transformador beta/metabolismo , Regulação para Cima , beta Catenina/genética
19.
Neoplasma ; 57(6): 594-600, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20845999

RESUMO

Recently oncogenetic human papilloma virus(HPV) infection has been suggested to promote laryngeal squamous cell carcinoma(LSCC). To determine the prevalence and genotypes of HPV infection in laryngeal cancer specimens, 84 specimens from pathologically confirmed LSCC patients were studied for the presence of viral DNA and possible virus integration into the cellular genome. HPV genotyping was assayed prior to the integration analysis by using two PCR-based assays, including HPV-16 and-18 E7 type-specific and L1 general primers (GP5+/GP6+). Additionally, a quantitative real-time PCR (qRT-PCR) was used to examine the physical status of HPV-16 or-18 in HPV positive LSCC. Using HPV L1 general primer amplification, HPV DNA was detected in 23 (27.4%) of the 84 LSCC samples. When PCR products were cloned and sequenced, HPV16 were found in all 23 L1 positive samples. However, when specific primers for HPV- 16 or -18 were used to amplify E6 and E7 in all samples, 29 cases (34.5%) were positive for HPV-16, while 6 cases (7.1%) were positive for HPV 18. Coinfection of HPV-16 and -18 were found in 4 cases (4.8%). In addition, qRT-PCR assay found that HPV-16 was characterized as episomal in 51.7% of cases, mixed (i.e., episomal and integrated) in 34.5%, and integrated in 13.8%, while HPV-18 was similarly characterized as episomal in 83.3% of cases and mixed in 16.7%. In the present study, about 36.9% of patients with LSCC were found to be infected with HPV-16 and -18 and integration of HPV-16 and -18 DNA into the host genome was found.


Assuntos
Carcinoma de Células Escamosas/virologia , Papillomavirus Humano 16/isolamento & purificação , Papillomavirus Humano 18/isolamento & purificação , Neoplasias Laríngeas/virologia , Adulto , Idoso , Proteínas do Capsídeo/genética , DNA Viral/análise , Proteínas de Ligação a DNA/genética , Feminino , Papillomavirus Humano 16/genética , Papillomavirus Humano 18/genética , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas Oncogênicas Virais/genética , Proteínas E7 de Papillomavirus/genética , Reação em Cadeia da Polimerase
20.
Cancer Gene Ther ; 13(6): 572-83, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16341141

RESUMO

E1B55K-deleted dl1520 could selectively replicate in cancer cells and has been used in clinical trials as an antitumor agent. The mechanism of virus selective replication in cancer cells, including a possible role of p53, is unclear. Studies with established cancer cell lines have demonstrated that some cancer cells are resistant to dl1520 replication, regardless of the p53 status. Hep3B cells supported the E1b-deleted adenoviruses to replicate, whereas Saos2 cells were resistant to viral replication. We applied p53-null Hep3B and Saos2 cells as models to clarify the replication ability of E1B55K-deleted adenoviruses with different expression levels of E1a. We show that lower E1A expression in Saos2 may be the reason for the poor replication in some cancer cells due to the fact that E1a promoter was less activated in Saos2 than in Hep3B. We also demonstrate that the E1B55K protein can increase E1A expression in Saos2 cells for efficient virus replication. In addition, the upstream regions of the E1a promoter have transcriptional activity in Hep3B cells but not in Saos2 cells. The viral E1B55K protein may activate cancer cellular factor(s) that targets the upstream regions of the E1a gene to increase its expression. This is the first study demonstrating that E1B55K protein affects the E1A production levels that is related to cancer selective replication. Our studies have suggested that increase of E1A expression from E1b-deleted adenoviruses may enhance killing cancer cells that otherwise are resistant to viral replication.


Assuntos
Adenoviridae/genética , Proteínas E1A de Adenovirus/metabolismo , Terapia Genética/métodos , Neoplasias/terapia , Replicação Viral , Adenoviridae/fisiologia , Proteínas E1A de Adenovirus/genética , Proteínas E2 de Adenovirus/genética , Ciclo Celular , Linhagem Celular Tumoral , Deleção de Genes , Vetores Genéticos , Humanos , Neoplasias/classificação , Neoplasias/patologia , Regiões Promotoras Genéticas , Regulação para Cima
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...