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Mycoses ; 61(2): 96-103, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28945326


A clear differentiation between pneumonia due to Pneumocystis jirovecii and "colonisation" is required for optimal case management. A quantification of fungal burden using major surface glycoprotein (MSG) gene-based real-time PCR was undertaken for the same. Lower respiratory tract samples collected from 104 patients of clinically suspected Pneumocystis pneumonia (PCP) were subjected to quantitative PCR using MSG gene. Based on whether or not the cases were treated for PCP, the efficacy of qPCR to differentiate between "diseased" and "colonised" was evaluated. Standard curve of plasmid-cloned gene and receiver operating characteristic curve defined a cut-off of Ct ≤ 25 to diagnose PCP and Ct within 26-39.3 range to depict colonisation. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the qPCR was 100%, each for diagnosing PCP. MSG-gene-based qPCR is a robust tool for the reliable differentiation of Pneumocystis pneumonia from colonisation.

Portador Sadio/diagnóstico , Glicoproteínas de Membrana/análise , Técnicas de Diagnóstico Molecular/métodos , Infecções por Pneumocystis/diagnóstico , Pneumocystis carinii/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Sistema Respiratório/microbiologia , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Glicoproteínas de Membrana/genética , Pessoa de Meia-Idade , Pneumocystis carinii/genética , Sensibilidade e Especificidade , Adulto Jovem
Phys Chem Chem Phys ; 15(39): 16686-92, 2013 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-23985964


In the present investigation, we report the effect of ytterbium (Yb) impurity band on charge transport and thermoelectric properties in PbTe1-xIx. The temperature dependent interaction of Yb-states with charge carriers and host energy-bands is found to significantly affect the electrical transport parameters in all the investigated samples. Our result indicates that, in the presence of Yb band, the carrier concentration did not increase as effectively as it was found in pristine PbTe with increasing iodine content. An anomalous switching of positive thermopower into negative was found in the samples with lower iodine content. Such phenomena were explained by the redistribution of charge carriers within the different available bands and promotion of vacancy like defects via substitutional impurity. Due to the optimum doping levels, the sample with the highest iodine content showed a decent figure of merit with a peak value of 0.69 at 575 K in the sample with x = 0.007.