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1.
Rev Environ Contam Toxicol ; 251: 1-24, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31011831

RESUMO

Heavy metal pollution in surface water is a global environmental problem. This study analyzed the trends, health risks, and sources of eight dissolved heavy metal species in river and lake water across five continents (Africa, Asia, Europe, North America, and South America; Oceania was excluded owing to a lack of data) for the period 1970-2017. We wanted to assess the effects of various implemented countermeasures to pollution and to determine those that could be adopted worldwide. Collectively, the water system showed increasing trends for Cd, Cr, Cu, Ni, Mn, and Fe and decreasing trends for Pb and Zn. The mean dissolved concentrations of most heavy metals were highest in Asia and lowest in Europe. Most heavy metals had low non-carcinogenic risks over this period. The cancer risks associated with Pb were lower than the hazardous level on all five continents over the five decades, whereas the cancer risks related to Cr exceeded the hazardous level in the 1970s, 2000s, and 2010s, and in Africa, Asia, and North America over the entire period. Mining and manufacturing were consistently found to be critical sources of metal pollution from 1970 to 2017. However, the heavy metal sources differed significantly by continent, with waste discharge and rock weathering dominant in Africa; mining and manufacturing, along with rock weathering, are dominant in Asia and South America; fertilizer and pesticide use, along with rock weathering, are dominant in North America; and mining and manufacturing, waste discharge, and rock weathering are dominant in Europe. Global trends in the metal loadings in water and in relevant pollution-control measures suggest that countermeasures in Europe have successfully controlled heavy metal pollution. The successful measures include implementing rigorous standards for metal emissions, limiting the metal concentrations in products, and rigorously treating metal-contaminated waste. Therefore, the measures implemented in Europe should be extended worldwide to treat heavy metal pollution in water.


Assuntos
Exposição Ambiental/análise , Metais Pesados/análise , Poluentes Químicos da Água/análise , Poluição Química da Água/estatística & dados numéricos , Exposição Ambiental/estatística & dados numéricos , Monitoramento Ambiental , Humanos , Lagos , Rios
2.
Biomed Mater ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31574486

RESUMO

Reconstruction of osteochondral tissues remains a challenging task in clinical therapy because of their heterogeneous structure. The best way to face the challenge is to develop a biomimetic construct to mimic the multilayered gradient from cartilage, to calcified cartilage and subchondral bone. In this study, bone marrow mesenchymal stromal cells (BMSCs) were cultured on electrospun fibrous meshes and cell sheets were incubated. The fibrous meshes were composed of 50% poly(L-lactide) and 50% gelatin, displaying excellent biocompatibility, cell affinity and degradability. Differentiation of BMSC sheets on fibrous meshes was induced chondrogenically or osteogenically. In particular, the BMSC sheets were able to be efficiently induced differentiating towards calcified cartilage by using a 1:1 (v/v) mixed medium of chondrogenic and osteogenic inductive media. Thus, a gradient three-dimensional construct was built by stacking the differently pre-differentiated cell/mesh complexes layer-by-layer from top-to-down to mimic the cartilage-to-bone transition. With this gradient construct being implanted in the rabbit knee osteochondral defect, it was confirmed that it could promote the tissue regeneration with intact cartilage layer formation in comparison with the multilayered construct while without a gradient. The strategy of using properly pre-differentiated BMSC sheet on fibrous mesh to build the osteochondral interface was thus suggested being feasible and effective in mimicking its hierarchical complexity, and favored the repairing of injured joint cartilage.

3.
ACS Appl Mater Interfaces ; 11(43): 40585-40591, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31589394

RESUMO

Developing new filters for the effective removal of high-temperature particulate matter is of great importance but still remains a challenge. Herein, we demonstrate a novel and facile strategy for producing hierarchical ceramic foams with three-dimensional interconnected porous architecture via the combination of chemical grafting of pore-forming agent and polyurethane foaming technique. Carbamate groups are directly grafted onto carbon black surface to enhance its dispersion. Abundant micrometer-sized pores are generated on the cell walls of porous frameworks to form three-dimensional interconnected porous architectures, resulting in the mullite foam with high particulate matter removal efficiency and relatively low pressure drop. The optimized mullite foam exhibits integrated properties of high particulate matter removal efficiency (96.7%), ultralow pressure drop (35 Pa), and outstanding recyclability. Our results open new opportunities for fabricating efficient particulate matter filters used in high-temperature environmental fields.

4.
Sci Rep ; 9(1): 13021, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31506559

RESUMO

A novel adsorbent (Fe3O4/HCO) was prepared via co-precipitation from a mix of ferriferrous oxide and a Ce-rich waste industrial sludge recovered from an optical polishing activity. The effect of system parameters including reaction time, pH, dose, temperature as well as initial concentration on the adsorption of Sb(III) were investigated by sequential batch tests. The Sb(III)/Fe3O4/HCO system quickly reached adsorption equilibrium within 2 h, was effective over a wide pH (3-7) and demonstrated excellent removal at a 60 mg/L Sb(III) concentration. Three isothermal adsorption models were assessed to describe the equilibrium data for Sb(III) with Fe3O4/HCO. Compared to the Freundlich and dubinin-radushkevich, the Langmuir isotherm model showed the best fit, with a maximum adsorption capacity of 22.853 mg/g, which exceeds many comparable absorbents. Four kinetic models, Pseudo-first-order, Pseudo-second-order, Elovich and Intra-particle, were used to fit the adsorption process. The analysis showed that the mechanism was pseudo-second-order and chemical adsorption played a dominant role in the adsorption of Sb(III) by Fe3O4/HCO (correlation coefficient R2 = 0.993). Thermodynamic calculations suggest that adsorption of Sb(III) ions was endothermic, spontaneous and a thermodynamically feasible process. The mechanism of the adsorption of Sb(III) on Fe3O4/HCO could be described by the synergistic adsorption of Sb (III) on Fe3O4, FeCe2O4 and hydrous ceric oxide. The Fe3O4/HCO sorbent appears to be an efficient and environment-friendly material for the removal of Sb(III) from wastewater.

5.
ACS Nano ; 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31518116

RESUMO

Nanofibrous aerogels constructed solely by ceramic components with temperature-invariant hyperelasticity could have broad technological implications in extreme environments. However, creating such materials has proven to be extremely challenging. Despite the results from laboratory, those aerogels are, unfortunately, still plagued with issues that would retard their further application: inferior structural integrity, failure at large compressive deformation, high production cost, and inability to withstand rigorous working conditions. To tackle these challenges, we report a facile strategy combining the chemical vapor deposition process and layer-by-layer self-assembly to construct hyperelastic SiC nanofibrous aerogels with three-dimensional porous architecture and improved structural integrity. The resultant aerogels outperform their natural counterparts and most state-of-the-art ceramic nanofibrous aerogels in their capability to quickly recover from large compressive deformation (50% strain), function in a wide range of temperatures, from -196 °C to 1100 °C in air, maintain high particle matter removal efficiency of >99.96%, and rapidly absorb various organic solvents and oils with high capacity and robust recoverability. Nanofibrous aerogels constructed by such a versatile method could provide fresh insights into the exploration of multifunctional nanofibrous aerogels for a variety of applications in extreme environments.

6.
Sensors (Basel) ; 19(19)2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31557982

RESUMO

Many kinds of weapon systems and launching equipment on the deck of large ships are easily affected by deck deformation. In order to ensure the accuracy of weapon systems and the safety of taking off and landing of carrier aircraft, a dynamic estimation method combining the main inertial navigation systems (INS) and the sub-inertial navigation systems (SINS) is designed to estimate the curvature and torsion of any trajectory on the deck. Our contributions start from the fact that the area of concern extends from the fixed points to any trajectory on the deck. The dynamic filter algorithm of wavelet combined with Kalman filter is used to process the acquired data. The wavelet method is used to remove the outliers in the acquired data, and the Kalman filter effectively reduces the influence of white noise, so that the estimation accuracy is guaranteed. The simulation results clearly show that the deck deformation of large ships can be obtained accurately in real-time over the observed area which proved that this dynamic inertial measurement method is feasible in practical engineering application.

7.
Soft Matter ; 15(38): 7583-7589, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31465079

RESUMO

Injectable hydrogels are adapted to irregularities in the desired location by injection as a liquid and gelation in situ. However, traditional slow-gelling injectable hydrogels may result in loss of cargo (cells/drugs) as well as diffusion at the target site, and extremely rapid gelation may lead to undesired premature coagulation. These practical problems can be solved by using self-healing hydrogels. Herein, through the reduction of disulfide bonds in BSA protein by using a reducing agent, the disulfide bonds between the individual BSA protein molecules are re-matched to form a network structure, thereby forming a protein hydrogel. This hydrogel shows an efficient and rapid self-healing property, and the broken protein hydrogel can be fast repaired within 1-2 minutes in response to H2O2 stimulation, and the repair efficiency reached up to 100%. The hydrogel can be extruded using only a pinhole syringe, and cytotoxicity experiments have demonstrated excellent biocompatibility of the protein hydrogel. This non-toxic, injectable, fast self-healing protein hydrogel is expected to be widely used in biomedical, tissue engineering, injectable gel, 3D bioprinting, and other applications.

8.
Cell Mol Life Sci ; 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31432232

RESUMO

Pancreatic ductal adenocarcinoma is prone to distant metastasis and is expected to become the second leading cause of cancer-related death. In an extremely nutrient-deficient and hypoxic environment resulting from uncontrolled growth, vascular disturbances and desmoplastic reactions, pancreatic cancer cells utilize "metabolic reprogramming" to satisfy their energy demand and support malignant behaviors such as metastasis. Notably, pancreatic cancer cells show extensive enhancement of glycolysis, including glycolytic enzyme overexpression and increased lactate production, and this is caused by mitochondrial dysfunction, cancer driver genes, specific transcription factors, a hypoxic tumor microenvironment and stromal cells, such as cancer-associated fibroblasts and tumor-associated macrophages. The metabolic switch from oxidative phosphorylation to glycolysis in pancreatic cancer cells regulates the invasion-metastasis cascade by promoting epithelial-mesenchymal transition, tumor angiogenesis and the metastatic colonization of distant organs. In addition to aerobic glycolysis, oxidative phosphorylation also plays a critical role in pancreatic cancer metastasis in ways that remain unclear. In this review, we expound on the intracellular and extracellular causes of the enhancement of glycolysis in pancreatic cancer and the strong association between glycolysis and cancer metastasis, which we expect will yield new therapeutic approaches targeting cancer metabolism.

9.
Medicine (Baltimore) ; 98(35): e16885, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31464917

RESUMO

BACKGROUND: This study aims to systematically assess the efficacy and safety of fasudil for the treatment of aneurysmal subarachnoid hemorrhage (ASH). METHODS: This study will include all of randomized controlled trials on the efficacy and safety of fasudil for the treatment of ASH. Ten electronic databases of PubMed, Embase, Cochrane Library, Google Scholar, Web of Science, Ovid, Cumulative Index to Nursing and Allied Health Literature, Allied and Complementary Medicine Database, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure will be searched from inception to the May 1, 2019 without language restrictions. We will also search gray literatures to avoid missing any other potential studies. Two authors will independently perform study selection, data extraction and management, and methodologic quality assessment. The primary outcome is limbs function. The secondary outcomes comprise of muscle strength, muscle tone, quality of life, and adverse events. RESULTS: This study will provide a comprehensive literature search on the current evidence of fasudil for the treatment of ASH from primary and secondary outcomes. CONCLUSION: The results of this study will present evidence to determine whether fasudil is an effective and safety treatment for patients with ASH. SYSTEMATIC REVIEW REGISTRATION: PROSPERO CRD42019136215.


Assuntos
1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/análogos & derivados , Aneurisma Intracraniano/complicações , Hemorragia Subaracnóidea/tratamento farmacológico , 1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/efeitos adversos , 1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/uso terapêutico , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto , Projetos de Pesquisa/normas , Hemorragia Subaracnóidea/etiologia , Resultado do Tratamento
10.
Arch Orthop Trauma Surg ; 139(11): 1617-1624, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31372713

RESUMO

PURPOSE: To identify the efficacy of isolated trochleoplasty (TP) as an independent treatment for severe trochlear dysplasia compared with TP combined with medial patellofemoral ligament (MPFL) reconstruction. METHODS: Search of current literature using terms (trochleoplasty and medial patellofemoral ligament reconstruction) in the electronic search engines PubMed and Embase, and Medline databases was performed on February 25, 2018, and it yielded 515 abstracts for review. At the end of the search, six articles met specific inclusion criteria and were included in this review. Means were calculated for population size, age and follow-up time. The Kujala score was analyzed as the primary clinical outcome parameter in the meta-analysis. Pooled estimates were calculated for postoperative complications. RESULTS: Six studies with a total of 192 knees (168 patients) were included in this analysis. The isolated TP group comprised of 3 articles with a total of 111 knees, and the TP combined with MPFL group comprised of 3 articles with a total of 81 knees. At the final follow-up, the preoperative Kujala score increased significantly by 21.39 (95% CI 18.94, 23.84; P < 0.00001) points in the isolated TP group and by 24.91 (95% CI 15.47, 34.36; P < 0.00001) points in the TP combined with MPFL group. The rates of subjective patellar instability including subluxation and anterior knee pain were 1.03% and8.45% respectively. Meanwhile, the rate of objective patellar redislocation was 2.06% in isolated TP group and 0% in TP combined with MFPL group. A total of 8.24% returned to the operating room for additional procedures in the isolated TP group and 7.04% in the TP combined with MPFL group. CONCLUSION: Trochleoplasty is a useful and reliable surgical technique to improve patellofemoral instability in patients with a dysplastic trochlea. However, it as isolated treatment for patients has lower outcome and higher residual instability compared with combined MPFL and trochleoplasty.

11.
Science ; 365(6456): 919-922, 2019 08 30.
Artigo em Inglês | MEDLINE | ID: mdl-31346137

RESUMO

Rhizobial infection and root nodule formation in legumes require recognition of signal molecules produced by the bacteria and their hosts. Here, we show that rhizobial transfer RNA (tRNA)-derived small RNA fragments (tRFs) are signal molecules that modulate host nodulation. Three families of rhizobial tRFs were confirmed to regulate host genes associated with nodule initiation and development through hijacking the host RNA-interference machinery that involves ARGONAUTE 1. Silencing individual tRFs with the use of short tandem target mimics or by overexpressing their targets represses root hair curling and nodule formation, whereas repressing these targets with artificial microRNAs identical to the respective tRFs or mutating these targets with CRISPR-Cas9 promotes nodulation. Our findings thus uncover a bacterial small RNA-mediated mechanism for prokaryote-eukaryote interaction and may pave the way for enhancing nodulation efficiency in legumes.

12.
Chin J Nat Med ; 17(6): 461-468, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31262458

RESUMO

In the present study, we investigated anti-inflammatory effect of Cardamine komarovii flower (CKF) on lipopolysaccharide (LPS)-induced acute lung injury (ALI). We determined the effect of CKF methanolic extracts on LPS-induced pro-inflammatory mediators NO and prostaglandin E2 (PGE2), production of pro-inflammatory cytokines (IL-1ß, TNF-α, and IL-6), and related protein expression levels of MyD88/TRIF signaling pathways in peritoneal macrophages (PMs). Nuclear translocation of NF-κB-p65 was analyzed by immunofluorescence. For the in vivo experiments, an ALI model was established to detect the number of inflammatory cells and inflammatory factors (IL-1ß, TNF-α, and IL-6) in bronchoalveolar lavage fluid (BALF) of mice. The pathological damage in lung tissues was evaluated through H&E staining. Our results showed that CKF can decrease the production of inflammatory mediators, such as NO and PGE2, by inhibiting their synthesis-related enzymes iNOS and COX-2 in LPS-induced PMs. In addition, CKF can downregulate the mRNA levels of IL-1ß, TNF-α, and IL-6 to inhibit the production of inflammatory factors. Mechanism studies indicated that CKF possesses a fine anti-inflammatory effect by regulating MyD88/TRIF dependent signaling pathways. Immunocytochemistry staining showed that the CKF extract attenuates the LPS-induced translocation of NF-kB p65 subunit in the nucleus from the cytoplasm. In vivo experiments revealed that the number of inflammatory cells and IL-1ß in BALF of mice decrease after CKF treatment. Histopathological observation of lung tissues showed that CKF can remarkably improve alveolar clearance and infiltration of interstitial and alveolar cells after LPS stimulation. In conclusion, our results suggest that CKF inhibits LPS-induced inflammatory response by inhibiting the MyD88/TRIF signaling pathways, thereby protecting mice from LPS-induced ALI.

13.
J Nanosci Nanotechnol ; 19(12): 7785-7789, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31196290

RESUMO

In the present work, NiO@NiO and NiO@Co³O4 core/shell composites have been fabricated by a facile hydrothermal process and investigated as electrodes for supercapacitors. The structure and the morphology of as-prepared samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results indicated that the NiO hollow spheres were decorated by NiO nanosheets and Co³O4 nanowires. Electrochemical properties were evaluated by cyclic voltammetry, galvanostatic charge/discharge measurements, and electrochemical impedance spectroscopy. According to the results NiO@NiO and NiO@Co³O4 core/shell composites exhibited high specific capacitance, being promising for high-performance supercapacitors.

14.
Zhongguo Zhong Yao Za Zhi ; 44(8): 1668-1673, 2019 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-31090333

RESUMO

To analyze the clinical characteristics of Maixuekang Capsules combined with traditional Chinese medicines in the treatment of patients with nephrotic syndrome,and provide references for improving rationality of clinical drug use. Based on the database of hospital information system(HIS) in 15 hospitals in China,the electrical medical records of the patients diagnosed as nephrotic syndrome and treated with Maixuekang Capsules were collected. Their diagnostic information and characteristics of combined traditional Chinese medicines were analyzed by using association rules. The results showed that 1 588 patients of nephrotic syndrome who used Maixuekang Capsules were often complicated with hypertension(863 cases,accounting for 7. 54%),anemia(551 cases,accounting for 4. 81%),and coronary heart disease(349 cases,accounting for 3. 05%). Maixuekang Capsules were mainly combined with Tabellae Rhei et Natrii Bicarbonatis,Baining Capsules,tanshinone,Ganmao Qingre Granule,Shuxuening Injection in treating nephrotic syndrome. The results indicated that in the real world,Maixuekang Capsules was mainly used in combination with traditional Chinese medicines such as blood-activating and stasis-removing agents,pathogens eliminating and supporting healthy Qi agents,digestants,anti-bacterial and anti-inflammatory agents,wind-dispersing and antipyretic agents for patients with nephropathy. By the pharmacological effect,it was suitable for nephropathy patients based on combined diagnosis. The association rules of combination were specific,and can provide reference for subsequent studies and rational clinical medication of traditional Chinese medicines.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Síndrome Nefrótica/tratamento farmacológico , Anemia/complicações , Cápsulas , China , Doença das Coronárias/complicações , Humanos , Hipertensão/complicações , Medicina Tradicional Chinesa
15.
Nat Commun ; 10(1): 1914, 2019 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-31015473

RESUMO

Degradation of extracellular matrix (ECM) underlies loss of cartilage tissue in osteoarthritis, a common disease for which no effective disease-modifying therapy currently exists. Here we describe BNTA, a small molecule with ECM modulatory properties. BNTA promotes generation of ECM components in cultured chondrocytes isolated from individuals with osteoarthritis. In human osteoarthritic cartilage explants, BNTA treatment stimulates expression of ECM components while suppressing inflammatory mediators. Intra-articular injection of BNTA delays the disease progression in a trauma-induced rat model of osteoarthritis. Furthermore, we identify superoxide dismutase 3 (SOD3) as a mediator of BNTA activity. BNTA induces SOD3 expression and superoxide anion elimination in osteoarthritic chondrocyte culture, and ectopic SOD3 expression recapitulates the effect of BNTA on ECM biosynthesis. These observations identify SOD3 as a relevant drug target, and BNTA as a potential therapeutic agent in osteoarthritis.


Assuntos
Anti-Inflamatórios/farmacologia , Benzamidas/farmacologia , Cartilagem Articular/efeitos dos fármacos , Matriz Extracelular/efeitos dos fármacos , Depuradores de Radicais Livres/farmacologia , Fatores Imunológicos/farmacologia , Osteoartrite/tratamento farmacológico , Sulfonamidas/farmacologia , Animais , Cartilagem Articular/imunologia , Cartilagem Articular/patologia , Condrócitos/efeitos dos fármacos , Condrócitos/imunologia , Condrócitos/patologia , Citocinas/genética , Citocinas/imunologia , Modelos Animais de Doenças , Progressão da Doença , Matriz Extracelular/imunologia , Matriz Extracelular/patologia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Humanos , Injeções Intra-Articulares , Masculino , Osteoartrite/genética , Osteoartrite/imunologia , Osteoartrite/patologia , Cultura Primária de Células , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/genética , Superóxido Dismutase/imunologia , Superóxidos/antagonistas & inibidores , Superóxidos/metabolismo , Transcriptoma/imunologia
16.
Arthroscopy ; 35(5): 1486-1497, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30979627

RESUMO

PURPOSE: To investigate platelet-rich plasma (PRP) combined with gelatin sponge (GS) to improve tendon-bone interface healing and structure formation. METHODS: Characterization of the GS scaffold was performed with a scanning electron microscope, and the release curve after loading with PRP was evaluated. A real-time reverse transcription quantitative polymerase chain reaction assay was performed to test the levels of tendon-to-bone healing-related gene expression. Finally, 18 New Zealand white rabbits were randomly divided into 3 groups and underwent semitendinosus autograft anterior cruciate ligament reconstruction: autograft group without PRP, PRP group, and PRP-GS group. All rabbits were killed 8 weeks after the operation. Magnetic resonance imaging scans, biomechanical testing, and histologic evaluation were performed. RESULTS: An enzyme-linked immunosorbent assay and cell counting kit-8 assay showed that the GS could control the release of PRP and prolong its bioactivity time, as well as promote bone marrow mesenchymal stem cell proliferation. In the PRP-GS group, the levels of related genes were upregulated compared with the PRP group (P < .05). Lower signal in the magnetic resonance images indicated fibrocartilage formation in the 2 groups with PRP. In addition, histologic staining showed that the tendon-bone connection had a greater fibrocartilaginous transition region in the PRP-GS group, and the histologic scores were higher (vs the PRP group, P = .039). The maximum failure load and stiffness were higher in the PRP-GS group than in the other 2 groups. CONCLUSIONS: GS loading with PRP could prolong the bioactivity time of PRP and promote bone marrow mesenchymal stem cell proliferation and osteogenic gene expression in vitro. It also promoted the early healing process at the tendon-bone junction in a rabbit anterior cruciate ligament reconstruction model. CLINICAL RELEVANCE: GS is a natural material and offers satisfactory biocompatibility. Using GS as a scaffold to control the release of bioactive factors in bone tunnels may be useful, but additional studies in human subjects will be necessary to evaluate its clinical prospects.

17.
Molecules ; 24(6)2019 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-30934545

RESUMO

Sonchus arvensis L. is a nutritious vegetable and herbal medicine that is consumed worldwide. The aim of this study was to evaluate the anti-fatigue effects and underlying effects of aqueous extract of Sonchus arvensis L. (SA). Male C57BL/6 mice from four groups designated vehicle, exercise, exercise with low dose (250 mg/kg) or high dose of SA (500 mg/kg), were trained by swimming exercise and orally administrated with SA every other day for 28 days. The anti-fatigue activity was determined by exhaustive swimming test, as well as the muscle structure, levels of blood hemoglobin, and metabolites including lactate and urea nitrogen. SA alleviated mice fatigue behaviors by eliminating metabolites, while improving muscle structure and hemoglobin levels. Moreover, SA enhanced glycogen synthesis of liver but not muscle via increasing GCK and PEPCK gene expressions. Importantly, SA improved antioxidant enzymes expression and activities in both liver and muscle, which was possibly related to its primary components polysaccharides and the antioxidant components including chlorogenic acid, luteolin, and chicoric acid. Taken together, the anti-fatigue effects of SA could be partly explained by its antioxidant activity and mediating effects on glycogen synthesis and metabolites elimination. Therefore, SA could be a potential nutraceutical for improving exercise performance and alleviating physical fatigue.


Assuntos
Fadiga/metabolismo , Extratos Vegetais/farmacologia , Sonchus/química , Animais , Biomarcadores , Fadiga/tratamento farmacológico , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Glicogênio/metabolismo , Histocitoquímica , Fígado/efeitos dos fármacos , Fígado/metabolismo , Imagem por Ressonância Magnética , Masculino , Camundongos , Atividade Motora/efeitos dos fármacos , Músculo Esquelético/diagnóstico por imagem , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Consumo de Oxigênio/efeitos dos fármacos , Condicionamento Físico Animal , Extratos Vegetais/química
18.
Knee Surg Sports Traumatol Arthrosc ; 27(11): 3543-3551, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30877317

RESUMO

PURPOSE: The purpose of this study was to evaluate whether a flattened bone tunnel has a positive effect on the tendon-bone healing (TBH) process in the early period after anterior cruciate ligament (ACL) reconstruction. METHODS: Seventy-two New Zealand White rabbits were randomly allocated into two groups, the flattened tunnel (FT) group and the conventional round tunnel (RT) group. We compared the cross-sectional areas and diameters of the bone tunnels between the two groups through computed tomography (CT) scanning. TBH results between the two groups were assessed by histological analysis, micro-CT scanning and biomechanical tests at 4 weeks, 8 weeks and 12 weeks after operation. RESULTS: The cross-sectional areas of the bone tunnels between the two groups were almost the same. However, the shape of bone tunnels in the FT group was more flattened. A faster cellular and collagen remoulding process were found in the FT group. Semiquantitative histological analysis of Safranin O staining showed that there was more fibrocartilage formation in the interface region in the FT group (P < 0.05). Sirius Red staining showed that the tissues in the interface areas were more intense in the FT group. Micro-CT scanning showed that more new bone formation could be found in the interface region in the FT group. The biomechanical tests also showed that FT ACL reconstruction will result in a stronger regenerated tendon-bone interface. CONCLUSIONS: Our study found that a flattened bone tunnel accelerated TBH in the early period after ACL reconstruction surgery in a rabbit model, which lays the groundwork for further clinical practice of this ACL reconstruction method.

19.
Life Sci ; 223: 110-119, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30878262

RESUMO

PURPOSE: Gastric cancer is a common malignancy worldwide, and is associated with high morbidity and mortality rates. Cordycepin is a 3'-deoxyadenosine drug with significant anti-cancer effects. The aim of this study was to determine the molecular mechanisms underlying cordycepin action on gastric cancer cell proliferation and migration. METHODS: The human gastric cancer cell lines MGC-803 and HGC-27 were treated with different concentrations of cordycepin (25 µM, 50 µM, 100 µM and 5 µM, 25 µM and 50 µM) for 48 h. Cell proliferation was assessed by MTT and colony formation assays, and in vitro migration by the wound healing and transwell assays. In addition, Flow Cytometry was used to detect the cell cycle and apoptosis. RT-PCR and Western blotting were used to evaluate the expression levels of key factors. RESULTS: Cordycepin significantly inhibited gastric cancer cell proliferation and migration in a dose-dependent manner, in addition to inducing apoptosis and arresting the cell cycle at the G2 phase. Mechanistically, cordycepin targeted the PI3K/Akt signaling pathway by significantly altering the expression levels/activation of several key mediators, and upregulated the anti-metastatic factor CLEC2. CONCLUSION: Cordycepin inhibited the proliferation and migration of gastric cancer cells by upregulating CLEC2 via the Akt signaling pathway.


Assuntos
Antineoplásicos/farmacologia , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Desoxiadenosinas/farmacologia , Lectinas Tipo C/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Gástricas/patologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Humanos , Transdução de Sinais , Neoplasias Gástricas/metabolismo
20.
J Hazard Mater ; 371: 233-242, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-30852275

RESUMO

The identification of hyperaccumulators is a key step for the phytoextraction of contaminated soils. However, few cadmium (Cd) hyperaccumulators have been identified in the plant kingdom. In our previous field investigations, Lantana camara L. plants exhibited some traits of hyperaccumulators. To confirm whether this species is a Cd hyperaccumulator, laboratory dose-gradient experiments and field sample analysis experiments were first designed and implemented in an integrated manner. The results showed that lantana plants did not exhibit any visible damage or marked reduction in shoot biomass when grown in Cd-contaminated soil with less than 100 mg kg-1 Cd. Moreover, the lantana plants exhibited high Cd tolerance with effective coordination of photosynthesis and rapid reactive oxygen species scavenging. Most importantly, the bioaccumulation factors (BFs) and translocation factors (TFs) were greater than 1.0 in all the Cd treatments, while the Cd concentrations in the shoots were all greater than those in the roots and were also greater than 100 mg kg-1, the threshold value for a Cd hyperaccumulator. Our data provide comprehensive evidence that lantana plants have the typical characteristics of a Cd hyperaccumulator and thus can be regarded as potential Cd-hyperaccumulating plants for the restoration of Cd-polluted soils.

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