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Huan Jing Ke Xue ; 39(6): 2600-2606, 2018 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965614


Many cities are located in the downstream areas of rivers. Rapid urbanization may result in drastic changes in the urban river ecosystem. Antibiotic resistant genes (ARGs) are considered to be emerging environmental pollutants, which may do harm to the health of humans and may pose a potential risk for urban ecological safety by the dissemination and enrichment of ARGs in urban rivers. In this study, a high-throughput quantitative PCR technique was used to investigate the diversity and abundance of ARGs at three sites in Lianjiang County and the estuary of the Aojiang River, Fujian Province. The results show the abundance of ARGs in the downstream urban area of Lianjiang County (3.9×1010 copies·L-1) is significantly higher than upstream of the urban area and the estuary of the Aojiang River. A total of 129 ARGs are detected in the downstream urban area, which is higher than in the upstream and the estuary. The results also suggest that ARGs are persistent and not easy to reduce in the natural river following the occurrence of ARGs and indicated that the urban river is an important reservoir of ARGs.

Huan Jing Ke Xue ; 37(11): 4402-4409, 2016 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964698


Sika deer breeding is rapidly developing in South China during the recent years; however, the impact of sika deer breeding on the abundance and diversity of antibiotic resistance genes (ARGs) in sika deer farm remains elusive. In this study, profiles of ARGs were investigated using high-throughput qPCR technique. The results showed that the abundance of ARGs varied among different samples in the following order, deer manure composting > deer manure > vegetable soil > pristine soil, and the profiles of ARGs in these samples were significantly different (P<0.05). The abundance of ARGs was significantly correlated to that of mobile genetic elements (MGEs), implicating the potential of horizontal transfer of ARGs, which might accelerate the dissemination and enrichment of ARGs in the environment of sika deer farm.

Farmacorresistência Bacteriana/genética , Genes Bacterianos , Microbiologia do Solo , Animais , Antibacterianos , China , Cervos , Fazendas , Esterco/microbiologia