Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 59
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Chem Inf Model ; 60(1): 17-21, 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31851496

RESUMO

Taking hydrogen bonds as a basis to explore biomolecular properties and interactions, we constructed the lone-pair electron (LPE) index and a molecular orbital fingerprint based on molecular hybrid orbitals to represent the ability of molecules to form hydrogen bonds. Then, a computational model was constructed to predict molecular interactions. The LPE and orbital fingerprint could effectively predict the biological properties and bioactivities of molecules. This study revealed the significance of hybrid orbitals for understanding cell biochemistry.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31831179

RESUMO

Alzheimer's disease (AD) as the first most neurodegenerative disease in the elderly still has no effective therapy, suggesting that the intervention toolbox for AD should be expanded. One newly developed strategy involves the use of photobiomodulation, such as near infrared or far infrared light, which has proven to attenuate AD-associated pathology. However, the efficacy of mid infrared light (MIR) in treating AD is under investigated. With this in mind, we assessed the benefits of MIR light of peak wavelength 7.7-10 µm treatment on APP/PS1 transgenic mice. We found that APP/PS1 mice treated with MIR light had improved learning and memory abilities and reduced amyloid-ß (Aß) plaque load in the brain. We also surprisingly found that the gut microbiota composition in APP/PS1 mice treated with MIR light returned to normal (wild type mice) levels. Together, these findings suggested a novel non-invasive and promising avenue for AD treatment via photobiomodulation, and also proposed that future target for AD might be the gut microbiota via the brain-gut-skin axis.

3.
JAMA ; 322(24): 2444-2445, 2019 12 24.
Artigo em Inglês | MEDLINE | ID: mdl-31860038
4.
J Food Sci ; 84(12): 3601-3607, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31730276

RESUMO

Sturgeon meat has been found to be suitable as surimi raw materials. The present study determined the modori phenomenon in sturgeon surimi gels and identified its relationship with cathepsins. In all heat-treated gels (25 to 90 °C, at 5 °C intervals), the 40 °C-incubated sturgeon surimi gel showed the weakest gel properties and water-holding capacity (P < 0.05), a rough protein gel network under SEM, and the highest protein solubility and trichloroacetic acid-soluble peptides content (P < 0.05). SDS-PAGE indicated that the myosin heavy chain band of sturgeon surimi gels was almost completely degraded at 40 °C. Moreover, the highest cathepsin L activity was observed in 40 °C-treated sturgeon surimi gels (P < 0.05). Our results suggested that the modori phenomenon in sturgeon surimi gels occurred at 40 °C, which was partially attributed to cathepsin L, thereby allowing for the better exploitation and utilization of sturgeon surimi.

5.
Molecules ; 24(16)2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31430953

RESUMO

Schizochytrium limacinum residue was hydrolyzed with various proteases (papain, trypsin, Flavourzyme, Protamex, and Alcalase 2.4L) to obtain antioxidative peptides. The results showed that the S. limacinum hydrolysates (SLHs) prepared with compound proteases (Protamex and Alcalase 2.4L) had the highest antioxidant activity, which was measured using methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability (IC50 = 1.28 mg/mL), hydroxyl radical scavenging ability (IC50 = 1.66 mg/mL), and reducing power (1.42 at 5.0 mg/mL). The hydrolysates were isolated and purified by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). Through analysis of electrospray ionization-mass spectrometer (ESI-MS/MS), the purified antioxidant peptide was identified as Pro-Tyr-Lys (406 Da). Finally, the identified peptide was synthesized for evaluating its antioxidant activity. The •OH scavenging ability and reducing power of Pro-Tyr-Lys were comparable to those of reduced L-glutathione (GSH). These results demonstrated that the antioxidant peptides from SLHs could potentially be used as effective antioxidants.


Assuntos
Antioxidantes/química , Microalgas/química , Peptídeos/química , Hidrolisados de Proteína/química , Compostos de Bifenilo/química , Depuradores de Radicais Livres/química , Glutationa/química , Hidrólise , Radical Hidroxila/química , Peroxidação de Lipídeos , Peptídeo Hidrolases/química , Picratos/química , Espectrometria de Massas por Ionização por Electrospray , Superóxidos/química
7.
Mar Drugs ; 17(6)2019 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-31181804

RESUMO

Zinc-binding peptides from oyster (Crassostrea gigas) have potential effects on zinc supplementation. The aim of this study was to prepare efficient zinc-binding peptides from oyster-modified hydrolysates by adding exogenous glutamate according to the plastein reaction and to further explore the zinc absorption mechanism of the peptide-zinc complex (MZ). The optimum conditions for the plastein reaction were as follows: pH 5.0, 40 °C, substrate concentration of 40%, pepsin dosage of 500 U/g, reaction time of 3 h and l-[1-13C]glutamate concentration of 10 mg/mL. The results of 13C isotope labelling suggested that the addition of l-[1-13C]glutamate contributed to the increase in the zinc-binding capacity of the peptide. The hydrophobic interaction was the main mechanism of action of the plastein reaction. Ultraviolet spectra and scanning electronic microscopy (SEM) revealed that the zinc-binding peptide could bind with zinc and form MZ. Furthermore, MZ could significantly enhance zinc bioavailability in the presence of phytic acid, compared to the commonly used ZnSO4. Additionally, MZ significantly promoted the intestinal absorption of zinc mainly through two pathways, the zinc ion channel and the small peptide transport pathway. Our work attempted to increase the understanding of the zinc absorption mechanism of MZ and to support the potential application of MZ as a supplementary medicine.


Assuntos
Técnicas de Química Analítica/métodos , Absorção Intestinal/efeitos dos fármacos , Ostreidae/química , Peptídeos/química , Peptídeos/farmacologia , Zinco/química , Zinco/metabolismo , Animais , Disponibilidade Biológica , Quelantes/química , Hidrolisados de Proteína/química
8.
Mol Nutr Food Res ; 63(18): e1900326, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31237989

RESUMO

SCOPE: Decreasing ß-amyloid (Aß) accumulation is of significance in finding therapeutic candidates for cognitive impairments in Alzheimer's disease (AD). The aim of this study is to investigate the potency of the active components of walnut protein in decreasing Aß aggregation and ameliorating cognitive impairments. METHODS AND RESULTS: Cell model of intracellular Aß42 aggregation is used to explore the active ingredients in walnut protein hydrolysate (WPH). A bioactive peptide (Pro-Pro-Lys-Asn-Trp, PW5) with great anti-Aß42 aggregation activity identified from the WPH is synthesized for in vitro and in vivo experiments. Using classic APP/PS1 mouse model, it is validated that PW5 exerts its effects on cognitive improvement through reducing Aß plaques accumulation. Moreover, metabolomic analysis reveals that serum norepinephrine (NE) and isovalerate levels are significantly increased in response to PW5 intervention, with decreased serum levels of acetylcholine (AChe) and valerate, compared with the vehicle-treated APP/PS1 mice. PW5 feeding also improves gut dysbiosis in APP/PS1 transgenic mice by increasing the relative abundance of Firmicutes and decreasing Proteobacteria and Verrucomicrobia as displayed by 16s rRNA analyses. CONCLUSIONS: These promising results support the utilization of peptide PW5 as an active ingredient in functional foods or potential drug candidate for the prevention and/or treatment of AD.

9.
Phytomedicine ; 61: 152842, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31048127

RESUMO

BACKGROUND: Parkinson's disease (PD) is an age-dependent progressive movement disorder characterized by a profound and selective loss of nigrostriatal dopaminergic neurons. Accumulation of -synuclein (-syn) positive protein aggregates in the substantia nigra is a pathological hallmark of PD, indicating that protein turnover defect is implicated in PD pathogenesis. PURPOSE: This study aims to identify neuroprotective compounds which can alleviate the accumulation of -syn in neuronal cells and dissect the underlying mechanisms. METHODS: High throughput screening was performed by dot blot assay. The degradation of different forms of -syn by candidate compounds were assessed by western blot. The autophagy lysosome pathway and ubiquitin-proteasome system were examined to dissect the degradation pathway. The UPS activity was assessed by cellular UPS substrates degradation assay and biochemical proteasome activity assay. Q-PCR was performed to test the mRNA level of different proteasome subunits. Furthermore, Neuroprotective effect of candidate compound was tested by LDH assay and PI staining. RESULTS: Through the high throughput screening, harmine was identified as a potent -syn lowering compound. The time-dependent and dose-dependent effects of harmine on the degradation of different forms of -syn were further confirmed. Harmine could dramatically promote the degradation of UPS substrates GFP-CL1, Ub-R-GFP and Ub-G76V-GFP, and activate cellular proteasome activity. Mechanistically, harmine dramatically enhanced PKA phosphorylation to enhance proteasome subunit PSMD1 expression. PKA inhibitor blocked the effects of harmine in activating UPS, up regulating PSMD1 and promoting -syn degradation, indicating that harmine enhances UPS function via PKA activation. Moreover, harmine efficiently rescued cell death induced by over-expression of -syn, via UPS-dependent manner. CONCLUSION: Harmine, as a new proteasome enhancer, may have potential to be developed into therapeutic agent against neurodegenerative diseases associated with UPS dysfunction and aberrant proteins accumulation.


Assuntos
Harmina/farmacologia , Neurônios/efeitos dos fármacos , Complexo de Endopeptidases do Proteassoma/metabolismo , Ubiquitina/metabolismo , alfa-Sinucleína/metabolismo , Animais , Autofagia/efeitos dos fármacos , Células Cultivadas , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Feminino , Camundongos Transgênicos , Neurônios/metabolismo , Células PC12 , Fosforilação/efeitos dos fármacos , Ratos , alfa-Sinucleína/genética
10.
Free Radic Biol Med ; 136: 135-145, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-30980888

RESUMO

Hyperuricemia is a metabolic disease caused by disorders of purine metabolism, the prevalence of which has increased worldwide. Here, a cell model for high uric acid production was established in vitro employing cultured human kidney cells (HK-2 cells), and its molecular basis was analyzed using gene expression profile. High performance liquid chromatography (HPLC) was used to monitor the content of metabolites in cell culture media. Adenosine addition was found to induce HK-2 cells to produce uric acid precursors (inosine and hypoxanthine). Furthermore, the cell model was verified by confirming the antihyperuricemic effect of the widely used antihyperuricemic drugs allopurinol, probenecid, and febuxostat, as well as reported bioactive peptides and amino acids, encompassing glutathione, tryptophan and carnosine, which significantly reduced uric acid production in the HK-2 cells (p < 0.05). RNA-Seq technology was used to perform a wide transcriptome analysis of the hyperuricemic cell model, and the results demonstrated that it has the potential to be used as a rapid and valid in vitro model to screen antihyperuricemic compounds that mimics in vivo cell growth patterns.

11.
Carbohydr Polym ; 213: 276-285, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30879670

RESUMO

The structure and bioactivity of a novel polysaccharide from Platycladus orientalis (L.) Franco leaves (POP2) were investigated in the present study. Structure characterization demonstrated that the average molecular weight of POP2 was 9.69 kDa and consisted of arabinose (14.39%), mannose (10.24%), glucose (63.95%) and galactose (11.42%). The main linkage types of POP2 consisted of (1→4)-linked α-d-Glc and (1→6)-linked α-d-Glc based on methylation and NMR analysis. Bioactivity evaluation showed that POP2 could effectively promote the secretion of inflammatory cytokines (IL-6 and TNF-α), as well as the anti-inflammatory cytokines (IL-10) in LPS-induced cells. Besides, the secretion of NO was significantly inhibited by POP2 in M1 model. POP2 could enhance the level of inflammatory cytokines (NO, IL-6 and TNF-α), while the secretion of the anti-inflammatory cytokine TGF-ß was markedly suppressed in IL-4 induced cells. Our work attempted to elucidate the regulation of macrophage polarization and support the potential application of POP2 as bioactive ingredient for functional foods.


Assuntos
Cupressaceae/química , Macrófagos/efeitos dos fármacos , Polissacarídeos/farmacologia , Animais , Polaridade Celular/efeitos dos fármacos , Células Cultivadas , Macrófagos/metabolismo , Camundongos , Folhas de Planta/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Células RAW 264.7
12.
J Food Sci ; 84(3): 667-677, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30779137

RESUMO

The polyphenols (PF) from Platycladus Orientalis (L.) Franco leaves were purified by using 10 different macroporous adsorption resins. HPD-722 resin showed the best adsorption and desorption capacities. The static and dynamic adsorption and desorption of PF on HPD-722 resin were studied and the total polyphenols were separated into two fractions, PF-A and PF-B. PF-A and PF-B demonstrated similar scavenging activity of free radical (DPPH, ABTS, hydroxyl radical, superoxide anion). The scavenging activity of PF-A and PF-B on hydroxyl radical and superoxide anion radical reached the equal levels of vitamin C and gallic acid. The IC50 value of PF-A for hydroxyl radical scavenging activity and superoxide anion radical scavenging activity were 0.50 and 0.56 mg/mL, while those of PF-B were 0.61 and 0.64 mg/mL. PF-A and PF-B could reduce the overproduction of inflammatory cytokines (TNF-α, Pro-IL-1ß, and IL-6) induced by lipopolysaccharide and their protein expression in THP-1 cells. PF-B exhibited better anti-inflammatory effect than PF-A in the dosage range of 1.0-4.0 µg/mL. Structural identification of PF-A and PF-B were conducted by HPLC-MS/MS. Ten polyphenol compounds were identified in PF-A and PF-B, respectively, by HPLC-MS/MS, including quercetin, apigenin, myricetin, and so on. Molecular docking studies indicated that apigenin, myricetin, luteolin, kaempferol, and quercetin effectively inhibit xanthine oxidase by forming hydrogen bonds with the amino acid residues and binding to the active site of the enzyme. The results might supply useful information for better understanding the chemical structure, antioxidant, and anti-inflammatory activities of Platycladuso (L.) Franco leaves polyphenols. PRACTICAL APPLICATION: This study demonstrated that polyphenols from P. orientalis (L.) Franco leaves have the potential applications as functional food ingredient for the prevention and treatment of gout and inflammation, hyperuricemia and gout.


Assuntos
Cupressaceae/química , Polifenóis/química , Antioxidantes , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Citocinas/genética , Citocinas/metabolismo , Depuradores de Radicais Livres , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Simulação de Acoplamento Molecular , Extratos Vegetais , Folhas de Planta/química , Superóxidos , Espectrometria de Massas em Tandem
13.
Front Pharmacol ; 10: 16, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30745870

RESUMO

Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the accumulation of protein aggregates (namely Lewy bodies) in dopaminergic neurons in the substantia nigra region of the brain. Alpha-synuclein (α-syn) is the major component of Lewy bodies in PD patients, and impairment of the ubiquitin-proteasome system has been linked to its accumulation. In this work, we developed a tetracycline-inducible expression system, with simultaneous induced expression of α-syn-EGFP and a bright red fluorescent protein marker (mCherry) to screen for potential compounds for degrading α-syn. We identified canthin-6-one as an α-syn lowering compound which promoted both wild type and mutants α-syn degradation in an ubiquitin-proteasome-system (UPS) dependent manner. By CRISPR/Cas9 genome-wide screening technology, we identified RPN2/PSMD1, the 26S proteasome non-ATPase regulatory subunit 1, as the targeting gene for pharmacological activity of canthin-6-one. Finally, we showed that canthin-6-one up-regulates PSMD1 and enhances UPS function by activating PKA.

14.
Food Funct ; 10(2): 1191-1202, 2019 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-30741289

RESUMO

Two novel peptides WW4 and WW7 were evaluated for their antioxidant activity, membrane penetrance and inhibiting activity of amyloid-ß protein (Aß) aggregation. The results showed that both WW7 (10.38 ± 0.22 µmol TE per µmol) and WW4 (6.32 ± 0.77 µmol TE per µmol) possessed a significant oxygen radical absorption capacity (ORAC) and strong 1,1-diphenyl-2-picrylhydrazyl (DPPH˙) scavenging capacity (WW7, IC50 0.05 ± 0.002; WW4, 1.06 ± 0.07). Interestingly, WW7 exhibited relatively higher antioxidant activity than WW4. In addition, both WW4 and WW7 showed high cell membrane penetrance characteristics in HEK293 cells. To measure the metabolic stability of WW4 and WW7 in cells, we labelled the peptides with FITC and then analyze the co-localization with lysosomes by imaging Flow-cytometry. We found that WW7 had a lower co-localization rate (1.39%) than WW4 (8.44%), indicating that WW7 was more stable than WW4. In vivo imaging assay demonstrated that WW7 presented higher metabolic stability with a much longer stability time (2687.33 ± 54.01 min) in BALB-c nude mice than WW4 (148 ± 26.85 min), which was consistent with the in vitro result. To illustrate the potential function of antioxidant capacity, an Aß aggregation cell model was applied to examine anti-Aß aggregation ability of WW4 and WW7. Surprisingly, WW7 (23.04 ± 13.64%) had stronger anti-Aß aggregation ability but WW4 did not show obvious potential, which was due to their structure difference. The present work would offer novel insight into the activity of antioxidants and anti-Aß aggregation, and uncover the under-appreciated function of peptides in effective application in AD therapy.


Assuntos
Peptídeos beta-Amiloides/química , Antioxidantes/farmacologia , Peptídeos/farmacologia , Sequência de Aminoácidos , Animais , Antioxidantes/química , Feminino , Células HEK293 , Humanos , Camundongos , Camundongos Nus , Peptídeos/química
15.
Langmuir ; 35(10): 3710-3716, 2019 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-30727729

RESUMO

Conventional chemotherapy, because of the high dose to keep the drug above the minimum effective concentration, possesses severe side effects and brings extra pain to patients. A controlled release drug delivery system, which is a bilayer self-assembled nanoparticle (NP) in this study, can solve this problem. Zein, a biodegradable natural protein from corn, was selected for the first layer of the drug encapsulation. The second layer was formed via the reversible ionic hydrogen bonds between zein and folic acid (FA), which was selected because of the two carboxylic acids and one amine group in its simple structure. Doxorubicin (DOX), a popular anticancer drug, was selected as the drug model to form the bilayer drug nanoencapsulation FA-NP-DOX. The in vitro controlled release profile of FA-NP-DOX was obtained. The in vivo pharmacokinetics and anticancer activity of FA-NP-DOX in tumor-xenografted animal models were also conducted. Compared to the zein nanoencapsulation of DOX (NP-DOX) and pure DOX, FA-NP-DOX showed comparable in vitro cytotoxicity but much longer in vitro controlled release time and in vivo circulation time. Both FA-NP-DOX and NP-DOX showed enhanced therapeutical efficiency in vivo than pure DOX. It is concluded that the bilayer self-assembled NP of zein and FA highly prolonged the controlled release and enhanced the therapeutic efficiency of the anticancer drug.

16.
Carcinogenesis ; 40(10): 1269-1277, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-30805585

RESUMO

Gastric cancer (GC) has high morbidity and mortality rates worldwide. Abundant literature has reported several individual genes and their related pathways intimately involved in tumor progression. However, little is known about GC progression at the gene network level. Therefore, understanding the underlying mechanisms of pathological transition from early stage to late stage is urgently needed. This study aims to identify potential vital genes and modules involved in the progression of GC. To understand the gene regulatory network of GC progression, we analyzed micro RNAs and messenger RNA s expression profiles by using a couple of bioinformatics tools. miR-205 was identified by differentially expressed analysis and was further confirmed through using multiple kernel learning-based Kronecker regularized least squares. Using weighted gene co-expression network analysis, the gastric cancer progression-related module, which has the highest correlation value with cancer progression, was obtained. Kyoto Encyclopedia of Genes and Genomes pathways and biological processes of the GCPR module genes were related to cell adhesion. Meanwhile, large-scale genes of GCPR module were found to be targeted by miR-205, including two hub genes SORBS1 and LPAR1. In brief, through multiple analytical methods, we found that miR-205 and the GCPR module play critical roles in GC progression. In addition, miR-205 might maintain cell adhesion by regulating SORBS1 and LPAR1. To screen the potential drug candidates, the gene expression profile of the GCPR module was mapped connectivity map (Cmap), and the mTOR inhibitor (Sirolimus) was found to be the most promising candidate. We further confirmed that Sirolimus can suppress cell proliferation of GC cell in vitro.

17.
J Agric Food Chem ; 66(44): 11812-11822, 2018 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-30339011

RESUMO

Prodrug, in which the inactive parent drug with good bioavailability is metabolized into an active drug in the body, is one of the main strategies to target the disease site to improve the drug efficiency and reduce the adverse effects of chemotherapy. Because of the good capability of chemical modification, zein, a plant derived protein, and drugs can be conjugated through environmentally sensitive links to form prodrugs capable of triggered drug release. In this study, a novel prodrug was synthesized using paclitaxel (PTX), zein, and a disulfide linker, and nanoparticles were formed by self-assembly of the prodrug. An effective in vitro triggered release, 80-90% in 5 min, of the prodrug based nanoparticles (zein-S-S-PTX_NP) was successfully approached. The cytotoxicity of zein-S-S-PTX_NP as well as the zein encapsulation of PTX (zein_PTX_NP) and pure PTX on HeLa cells and NIH/3T3 fibroblast cells was tested using MTS assay. It showed that, after the treatment of zein-S-S-PTX_NP at the equivalent PTX concentrations of 0.1, 0.5, 1, and 5 µg/mL, respectively, zein-S-S-PTX_NP had zero damage to normal cells but a similar cytotoxicity to cancer cells as pure PTX. In the animal study, the tumor was 50% of the original size after the treatment of zein-S-S-PTX_NP for 9 days with 3 doses. This study suggested that the novel prodrug based nanoparticle zein-S-S-PTX_NP could be a promising approach in chemotherapy with targeted delivery, improved efficacy, and reduced side effects.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Sistemas de Liberação de Medicamentos/métodos , Paclitaxel/administração & dosagem , Paclitaxel/química , Pró-Fármacos/administração & dosagem , Pró-Fármacos/química , Zeína/química , Animais , Linhagem Celular Tumoral , Portadores de Fármacos/farmacologia , Sistemas de Liberação de Medicamentos/instrumentação , Liberação Controlada de Fármacos , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Nanopartículas/química , Oxirredução
18.
Peptides ; 107: 45-53, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30077718

RESUMO

Traditional drugs used to treat hyperuricemia have adverse effects. In this study, to identify safer anti-hyperuricemic bioactive peptides isolated from food-derived protein hydrolysates, a hyperuricemia rat model induced by potassium oxonate (PO) was used to evaluate the activity of bonito hydrolysates (BH), dephenolised walnut hydrolysates (DWH), and soybean hydrolysates (SH). The serum uric acid level of rats in the BH group (95.4 ± 27.4 µM, p < 0.01) was significantly reduced compared to that in the model group (212.00 ± 30.00 µM) to a level even lower than that in allopurinol group (114.3 ± 53.0 µM). Furthermore, BH alleviated renal impairment caused by PO in vivo and exhibited the greatest xanthine oxidase (XOD) inhibitory activity (65.5 ± 8.0%) in vitro compared to the other hydrolysates. Two peptides identified from BH bound the catalytic site of XOD, among which the hydrophobic peptide WML entered the active site of XOD more easily compared to the hydrophilic peptide PGACSN, possibly because of hydrophobic interactions. The chemically synthesized WML demonstrated high XOD inhibitory effect compared to PGACSN and a significant change in the secondary structure of XOD. Therefore, hexapeptide PGACSN and tripeptide WML are partially responsible for the anti-hyperuricemic activity of BH, and hydrophobic amino acids play important roles in the XOD inhibitory activity of peptides.


Assuntos
Hiperuricemia/tratamento farmacológico , Peptídeos/farmacologia , Xantina Oxidase/antagonistas & inibidores , Animais , Modelos Animais de Doenças , Produtos Pesqueiros/análise , Hiperuricemia/enzimologia , Juglans/química , Masculino , Hidrolisados de Proteína/farmacologia , Ratos , Ratos Sprague-Dawley , Soja/química
19.
Food Funct ; 9(8): 4508-4517, 2018 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-30083676

RESUMO

There are two long-standing issues that are holding back the full exploitation of food-based double emulsions: (i) unavailability of large-scale equipment to ensure efficient nondestructive two-step emulsification and (ii) limited food-grade ingredients available to replace polyglycerol polyricinoleate (PGPR) as the primary emulsifier. To overcome these, a facile one-step emulsification strategy was developed to generate a food-grade W/O/W double Pickering emulsion by using corn-peptide-functionalized calcium phosphate (CP-CaP) particles as the emulsifier. It was demonstrated that the wettability of such CP-CaP particles can be tuned through modulation of the oil phase composition. The incorporation of health benefiting ω-3 oils (algal oil) or essential polyunsaturated fatty acids (linoleic acid and linolenic acid) into common vegetable oils leads to the hydrophobization of a fraction of CP-CaP particles through in situ adsorption of the free fatty acids, which provide satisfactory stabilization of both O/W and W/O interfaces, thus generating stable double Pickering emulsions. Moreover, the algal oil-loaded double Pickering emulsions that incorporate water-soluble isoascorbic acid show improvement in both their oxidative stability and flavor properties. This study demonstrated that the edible CP-CaP particle based double Pickering emulsions have promising potential to be applied in the food industry.


Assuntos
Emulsificantes/química , Manipulação de Alimentos/instrumentação , Óleos Vegetais/química , Emulsões/química , Tamanho da Partícula , Molhabilidade
20.
J Antimicrob Chemother ; 73(10): 2652-2661, 2018 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-29986036

RESUMO

Objectives: To elucidate the phylogenetic relationships among ST9-MRSA-XII isolates from different sources and their genetic features in colonization of different hosts. Methods: We obtained whole-genome sequences of two ST9-MRSA-XII isolates from nasal swabs associated with live pigs in China, and compared them with 135 previously sequenced genomes of 78 human-associated, 39 bovine and 18 porcine Staphylococcus aureus consisting of 11 MRSA of SCCmecXII, 62 MRSA of other SCCmec types and 62 MSSA. The distribution of diverse mobile genetic elements (MGEs), resistance genes and virulence determinants was investigated in relation to isolate phylogeny. Comparisons of SNPs and small insertion/deletions (indels) were conducted to examine genome-level variation between porcine and bovine ST9-MRSA-XII. Results: Phylogenetic analysis revealed that both of our porcine ST9-MRSA-XII isolates clustered with porcine, bovine and human-associated ST9-MRSA-XII. All of these isolates possessed a novel type V pathogenicity island, νSaα, carrying the von Willebrand-binding protein gene vwb, the immune evasion complex gene scn, the aminoglycoside resistance gene aadE, staphylococcal superantigen-like genes (ssl1-ssl11) and lpl tandem genes. Compared with bovine ST9-MRSA-XII BA01611, our porcine isolates contain non-synonymous nucleotide substitutions in genes encoding adhesins and an indel located in a phosphonate ABC transporter pseudogene. Conclusions: The data suggest transmission of ST9-MRSA-XII among swine, cattle and humans. The extraordinary success of the ST9-MRSA-XII group in colonization of various hosts is likely due to acquisition of many MGEs harbouring functional antimicrobial resistance and virulence genes. Transmission of ST9-MRSA-XII between porcine and bovine hosts was accompanied by changes in binding profile and function in genes involved in metabolism.


Assuntos
Staphylococcus aureus Resistente à Meticilina/classificação , Filogenia , Infecções Estafilocócicas/veterinária , Doenças dos Suínos/transmissão , Sequenciamento Completo do Genoma , Animais , Antibacterianos/farmacologia , Técnicas de Tipagem Bacteriana , Bovinos/microbiologia , China , Humanos , Sequências Repetitivas Dispersas , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Testes de Sensibilidade Microbiana , Tipagem de Sequências Multilocus , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA , Infecções Estafilocócicas/transmissão , Suínos/microbiologia , Doenças dos Suínos/microbiologia , Fatores de Virulência
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA