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1.
Food Chem ; 400: 134062, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36075165

RESUMO

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are emerging pollutants that threaten food safety. Herein, a rapid, accurate, and selective method for determination of PFOA and PFOS in milk was established by using new molecularly imprinted phenolic resin (MIP-PR) as the adsorbent of dispersive filter extraction (DFE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). MIP-PR was synthesized at room-temperature using m-aminophenol, glutaraldehyde, and perfluorononanoic acid as the monomers, cross-linkers, and virtual templates, respectively, and exhibited rapid mass transfer (30 s), high selectivity (imprinted factors > 3.7), and large adsorption capacity (>54.6 mg/g). Compared with reported methods, the developed MIP-PR-DFE method is fast, selective, inexpensive, and shows good purification and enrichment efficiency. The proposed MIP-PR-DFE-LC-MS/MS exhibited low limits of detection (0.006-0.022 ng/mL), high recoveries (94.7-109 %), and good precision (RSDs ≤ 9.5 %). This study provides a new idea for the development of imprinted resin adsorbents for perfluorinated compound, and a new method for sample pretreatment for monitoring of PFOA and PFOS in food.


Assuntos
Poluentes Ambientais , Impressão Molecular , Ácidos Alcanossulfônicos , Animais , Caprilatos , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida , Poluentes Ambientais/análise , Fluorcarbonetos , Formaldeído , Glutaral , Leite/química , Impressão Molecular/métodos , Fenóis , Polímeros , Extração em Fase Sólida , Espectrometria de Massas em Tandem/métodos
2.
Opt Lett ; 47(18): 4834-4837, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36107102

RESUMO

Color-tunable upconversion luminescence has wide prospects for anti-counterfeiting and disease diagnosis/treatment. To date, achieving high-quality tunable red and blue emissions using a single excitation wavelength remains a formidable challenge, due to the large energy difference between the red and blue photons. In this Letter, based on Tm3+ upconversion luminescence, blue dominant and red dominant emissions are generated upon 980-nm excitations using a short and long pulse, respectively. The corresponding color tuning mechanisms are investigated based on the spectral observations. The proposed color tuning strategy is particularly useful for in vivo applications as the red and blue lights play important roles in biological imaging and drug release, respectively.


Assuntos
Luz , Luminescência , Fótons
3.
Food Chem ; 401: 134133, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36113217

RESUMO

Improving detection sensitivity is still a major research emphasis for lateral flow immunoassay (LFIA). Increasing the binding efficiency and stability of the probe is an achievable and effective solution. In this work, we developed a highly sensitive lateral flow immunoassay for clenbuterol detection by using bismuth sulfide nanoparticle (Bi2S3) nanoparticles (NPs) as a novel marker. Here, Bi2S3 NPs can link with the antibody by hydrogen bonding to improve the performance of the probe, e.g., stability and sensitivity. Benefiting from the direct hydrogen bonding between Bi2S3 NPs and the monoclonal antibody (mAb), high sensitivity is obtained by the proposed LFIA with a lower visible detection limit of 0.1 ng mL-1 and a cut-off value of 4 ng·mL-1 for CLE detection, which is 5-fold and 7.5-fold improved than the conventional Au NPs based LFIA. In addition, the encouraging practical application results in milk, pork, and beef show that the bismuth sulfide nanoparticle has a great popularizing potential in the performance promotion of LFIAs for food safety monitoring.

4.
Eur J Radiol ; 156: 110504, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36108474

RESUMO

OBJECTIVE: To evaluate and compare the diagnostic performance of apparent diffusion coefficient (ADC) values and MRI-based radiomics analysis for lymph node metastasis (LNM) detection in patients with cervical cancer (CC). METHODS: We searched relevant databases for studies on ADC values and MRI-based radiomics analysis for LNM detection in CC between January 2001 and December 2021. Methodological quality assessment of risk of bias using Quality Assessment of Diagnostic Accuracy Studies 2 and radiomics quality score (RQS) of the studies was conducted. The pooled sensitivity, specificity, positive likelihood ratio (LR+), negative likelihood ratio (LR-), diagnostic odds ratio (DOR), and area under the curve (AUC) were calculated. Diagnostic performance was compared between the two quantitative analyses using a two-sample Z-test. RESULTS: In total, 22 studies including 2314 patients were included. Unclear risk of bias was observed in 4.5-36.4% of the studies. The 8 radiomics studies exhibited a median (interquartile range) RQS of 13.5 (5.5-15.75). The pooled sensitivity, specificity, LR+, LR-, DOR, and AUC of the ADC values vs radiomics analysis were 0.86 vs 0.84, 0.85 vs 0.73, 5.7 vs 3.1, 0.17 vs 0.22, 34 vs 14, and 0.91 vs 0.86, respectively. There was no threshold effect or publication bias, but significant heterogeneity existed among the studies. No significant difference was detected in the diagnostic performance of the two quantitative analyses using the Z-test. CONCLUSION: ADC values are more clinically promising because they are more easily accessible and widely applied, and exhibit a non-statistically significant trend to outperform radiomics analysis.

5.
Front Neurol ; 13: 937417, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119700

RESUMO

Purpose: To identify the most important factors affecting physician decision-making regarding antiplatelet therapy. Methods: We retrospectively gathered data from minor ischemic stroke patients with NIHSS scores ≤ 5 within 72 h of onset from 2010 to 2018. The population was divided into four groups by initial antiplatelet therapy: aspirin monotherapy (AM), dual antiplatelet therapy with aspirin and a loading dose of clopidogrel (clopidogrel loading dose of 300 mg on the first day; DAPT-ALC), dual antiplatelet therapy with aspirin and no loading dose of clopidogrel (clopidogrel 75 mg daily, no loading dose; DAPT-AUC), and clopidogrel monotherapy (CM). Results: In total, 1,377 patients were included in the analysis (excluding patients who accepted thrombolytic drugs, participated in other clinical trials, or had not used antiplatelet drugs). The mean ± S.D. age was 62.0 ± 12.7 years; 973 (70.7%) patients were male. The four groups were AM (n = 541, 39.3%), DAPT-ALC (n = 474, 34.4%), DAPT- AUC (n = 301, 21.9%), and CM (n = 61, 4.4%). Patients receiving antiplatelet monotherapy were older than those receiving dual antiplatelet therapy (63.7-65.7 vs. 59.6-61.4 years), and the median initial systolic blood pressure level was higher in the DAPT-ALC group than in the other groups (all P < 0.05). Patients under 75 years old with an admission SBP lower than 180 mmHg, a history of AM, coronary heart disease, no history of intracerebral hemorrhage, stroke onset occurring after guideline recommendations were updated (the year of 2015), onset-to-arrival time within 24 h, and initial NIHSS score ≤ 3 were more likely to take DAPT-ALC than AM. Compared with DAPT-ALC, DAPT-AUC was associated with an initial SBP level lower than 180 mmHg, a history of smoking, hypertension, no history of ICH, previous treatment with antihypertensives, and onset year after the recommendations were updated. Conclusions: Many factors affect doctors' decisions regarding antiplatelet therapy, especially guidelines, age, admission SBP level, and hypertensive disease.

6.
J Ethnopharmacol ; : 115695, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36108894

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Veronicastrum Heist. ex Fabr. (Plantaginaceae) is a multifunctional plant in China and other parts of the continent. It has traditionally been used in the treatment of ascites, edema, blood stasis, pain relief, chronic nephritis injury, fever, cough, headache, arthritis, dysentery, rheumatism, pleural effusion, liver damage, and other disorders. Although research has confirmed that the genus Veronicastrum contain many active compounds, no review of its traditional uses, phytochemistry or pharmacology has been conducted to date. AIM: This review aims to systematically evaluate the traditional uses, phytochemistry, and pharmacology of the genus Veronicastrum, discuss its medicinal potential, modern scientific research, and the relationship between them, and put forward some suggestions to promote further development and utilization of Veronicastrum. MATERIALS AND METHODS: The traditional uses, phytochemical and pharmacological data related to the genus Veronicastrum from 1955 to date was compiled by surveying the ethnomedicinal books and published papers, and searching the online databases including Google Scholar, China National Knowledge Infrastructure (CNKI), Science Direct, Web of Science and World Flora Online. RESULTS: Species of the genus Veronicastrum are widely used in folkloric medicine and some of their uses have been confirmed in modern pharmacological activities. A total of 89 chemical constituents have been isolated from the genus Veronicastrum, including flavonoids, carbohydrates, iridoids, terpenoids, phytosterols, phenolic acids, and other constituents. Among the compounds isolated, iridoids, flavonoids, and terpenoids are responsible for the biological activities of this genus with significant pharmacological activities both in vitro and in vivo. The extracts and compounds isolated from this genus have been reported to contain a wide range of pharmacological activities such as immunosuppressive, antioxidant, anti-cancer, anti-inflammatory, gastro protective, and antimicrobial activity. CONCLUSION: The genus Veronicastrum is not only a great herbal remedy, but also has numerous bioactive chemicals with potential for new drug discovery. In the literature, phytochemical investigations have been undertaken on five species. Detailed scientific research is still needed to fully understand this genus. Furthermore, its bioactive chemicals' structure-activity connection, in vivo activity, and mechanism of action ought to be investigated further.

7.
Can Respir J ; 2022: 4201786, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36060828

RESUMO

Impairment of pulmonary function was evaluated in chronic bronchitis patients with preserved ratio impaired spirometry (PRISm). We retrospectively collected clinical data from 157 chronic bronchitis (CB) and 186 chronic obstructive pulmonary disease (COPD) patients between October 2014 and September 2017. These patients were assigned to three groups: control (normal pulmonary function), PRISm (forced expiratory volume in 1 second [FEV1]/forced vital capacity [FVC] ≥ 0.7, FEV1 < 80% of predicted value), and COPD (FEV1/FVC <0.7) groups. Because small airway function was the main focus, in the COPD group, only patients in accordance with the Global Initiative for Chronic Obstructive Lung Disease (GOLD) grades 1 and 2 were included. Evaluation of pulmonary function (including impulse oscillometry) was performed and compared among these groups. Compared with the control group, the PRISm and COPD groups showed statistically significant differences in the predicted FEV1% (p < 0.001), maximal expiratory flow (MEF) 25% (p < 0.001), MEF50% (p < 0.001), maximal midexpiratory flow (MMEF) 25-75% (p < 0.001), residual volume (RV)/total lung capacity (TLC; p < 0.001), FVC% (p < 0.001), total respiratory resistance and proximal respiratory resistance (R5-R20; p < 0.001), respiratory system reactance at 5 Hz (X5; p < 0.001), resonant frequency (Fres; p < 0.001), and area of reactance (Ax; p < 0.001). However, the predicted FEV1% and RV/TLC were similar between the PRISm and COPD groups (p=0.992 and 0.122, respectively). PRISm is a nonspecific pattern of pulmonary function that indicates small airway dysfunction and may increase the risk of transformation to obstructive ventilation dysfunction. This trial is registered with ChiCTR-OCH-14004904.


Assuntos
Bronquite Crônica , Doença Pulmonar Obstrutiva Crônica , Volume Expiratório Forçado , Humanos , Pulmão , Oscilometria , Estudos Retrospectivos , Espirometria
8.
Artigo em Inglês | MEDLINE | ID: mdl-36064144

RESUMO

OBJECTIVE: The purpose of this study is to investigate the clinical effect of Dynamic real-time navigation to assist immediate implant without flapping in the esthetic zone. METHODS: Eight patients who underwent immediate implantation in the aesthetic area were included. A total of 11 implants were implanted using dynamic real-time navigation system combined with non-flap technology. Clinical indicators including implant deviation, initial stability, alveolar bone absorption, implant success rate, pink esthetic score (PES), Papilla index score (PIS), and the thickness of labial side bone plate of the implant were recorded. RESULTS: The deviation between the actual implant position and the preoperative design was (0.76±0.08) mm at the top, (1.11±0.18) mm at the root, (0.90±0.16) mm at the depth, and (1.48±0.91)°at the Angle. ISO values of all implants were greater than 59. PES was greater than 8. PIS index was 2 or 3. The average alveolar bone absorption was (0.34±0.09) mm and the thickness of bone plate on the lip of implant was greater than 1.6 mm. The success rate of implantation was 100%. CONCLUSION: The use of dynamic real-time navigation assisted non-flap implantation in the aesthetic area can effectively reduce implant deviation and improve the aesthetic effect.

9.
Sci Total Environ ; 854: 158713, 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36113791

RESUMO

Heavy metal exposure via food consumption is inadequately investigated and deserves considerable attention. We collected hundreds of food ingredients and daily meals and assessed their probabilistic health risk using a Monte Carlo simulation based on an ingestion rate investigation. The detected concentrations of four heavy metals (Cr, Cd, Pb, and Hg) in all daily meal samples were within the limits stipulated in the National Food Safety Standard (GB 2762-2017), while that for As level was excessive in 0.3 % of daily meal samples. The same results were also observed in most food ingredient samples, and a standard-exceeding ratio of 23 % of As was observed in aquatic food or products, especially seafood, which was with the highest concentration reaching 1.24 mg/kg. Combining the detected heavy metal amounts with the ingestion rate investigation, the hazard quotients (HQs) of As, Cr, Cd, Pb, and Hg in daily meals and food ingredients were all calculated as lower than 1 (no obvious harm), while the incremental lifetime cancer risk (ILCR) of As and Cr (>1 × 10-4), indicating that the residual As posed potential health effects to human health. It was noteworthy that the proportion of aquatic foods only accounted for 6.3 % of daily meals, but they occupied 41.1 % of the heavy metal exposure, which could be attributed to the high amounts of heavy metals in aquatic foods. This study not only provided basic data of heavy metal exposure and potential health risks through daily oral dietary intake, but also illuminated the contribution of different kinds of food ingredients. Specifically, the study highlighted the contamination of aquatic foods with As, especially seafood such as shellfish and bivalves.

11.
Colloids Surf B Biointerfaces ; 218: 112743, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35961113

RESUMO

In this study, novel active materials based on cellulose acetate (CA) and gelatin (Gel) for the release of eugenol (Eg) were developed by fabricating CA/Gel-Eg core-shell structured nanofiber films using the coaxial electrospinning technique. These electrospun nanofibers were coated with a CA shell, which has excellent water stability. Eg was encapsulated on the core part of the Gel. CA/Gel-Eg fiber morphology, structure, thermal, surface wettability, mechanical, encapsulation efficiency, release profile, and biological activity were evaluated. Results showed that the Eg-loaded nanofibers had uniform fiber morphologies and average fiber diameters ranging from 302.5 to 166.6 nm. Eg could be efficiently encapsulated in nanofibers and released for 120 h. Additionally, the CA/Gel-Eg core-shell structured nanofiber films displayed good Eg dose-dependent antibacterial activity. These findings could lead to a new strategy for developing electrospun fibers with a core-shell structure for active food packaging applications.


Assuntos
Nanofibras , Antibacterianos/farmacologia , Celulose/análogos & derivados , Eugenol , Gelatina , Nanofibras/química , Água
12.
Virol Sin ; 2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-35961502

RESUMO

Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is one of the most devastating diseases in the global pig industry due to its high mortality rate in piglets. Maternal vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV challenge. From 2017 to 2021, we collected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of PEDV. The result showed that about 52.15% (158/303) of the farms were positive for PEDV with an overall detection rate of 63.95% (564/882) of the samples. The S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis. A total of 71 PEDV strains (68.27%) sequenced in this study were clustered into the predominant G2c subgroup, while the newly-defined G2d strains (9.62%) were identified in three provinces of China. The NH-TA2020 strain of G2c subgroup was isolated and cultured, and its infection to piglets caused watery diarrhea within 24 â€‹h, indicating its strong pathogenicity. Oral administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in colostrum. The piglets fed by the gilts above were challenged with NH-TA2020 strain or CH-HeB-RY-2020 strain from G2d subgroup, and the clinical symptoms and virus shedding were significantly reduced compared to the mock group. Our findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to 2021. Oral administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses, which confer protection against the homologous and emerging G2d PEDV strains challenges in neonates.

13.
Artigo em Inglês | MEDLINE | ID: mdl-36004621

RESUMO

INTRODUCTION: The present study aimed to investigate the effect of homeodomain interacting protein kinase 2 (HIPK2) on pulmonary fibrosis and the probable mechanisms. MATERIAL AND METHODS: We constructed a mouse model of bleomycin-induced pulmonary fibrosis and up-regulated the expression of HIPK2 in the lung by in vivo transfection. Lung tissues were collected for the detection of mesenchymal markers (α-SMA, collagen I, collagen III, etc.) and the expression of ß-catenin by RT-PCR, western blot, and immunohistochemistry. Mouse lung fibroblasts (MLFs) with upregulation or downregulation of HIPK2 were successfully constructed and XAV939 was used to downregulate ß-catenin expression. Then, we evaluated the activation of MLFs and the Wnt/ß-catenin pathway under various conditions. RESULTS: The results showed that in the bleomycin-induced mouse model group, the lung alveolar structure was severely damaged, the number of collagen fibers was increased in alveolar speta, and the expression of HIPK2 in the fibrotic area was found to be reduced. After upregulating HIPK2 in the lungs of the mouse fibrosis model we found that pulmonary fibrosis was attenuated and the expression of ß-catenin and mesenchymal markers was reduced. The upregulation of HIPK2 inhibited the proliferation and migration of MLFs induced by TGF-ß1, promoted apoptosis of MLFs, and reduced the expression of mesenchymal markers and ß-catenin. Meanwhile, downregulation of HIPK2 promoted the proliferation and migration of MLFs, inhibited apoptosis, and promoted mesenchymal markers and ß-catenin expression. XAV939 treatment of MLFs silencing HIPK2 inhibited their proliferation and activation via silencing HIPK2, promoted apoptosis, and reduced interstitial markers and ß-catenin expression. CONCLUSIONS: HIPK2 can attenuate bleomycin-induced pulmonary fibrosis by inhibiting the Wnt/ß-catenin pathway in mouse lung fibroblasts.

14.
Zhongguo Zhen Jiu ; 42(8): 923-6, 2022 Aug 12.
Artigo em Chinês | MEDLINE | ID: mdl-35938337

RESUMO

With 12 representative states such as Ohio and Illinois as examples, the disciplinary system for acupuncturists in the United States is introduced. The disciplinary system mainly covers several aspects such as the subject of the implementation, the types of illegal acts, the form of disciplinary responsibility, and the hearing procedure. The acupuncture industry association has the responsibility of disciplinary action in most states. Corresponding disciplinary measures will be taken depending on the illegal activities of acupuncturists according to the types of illegal practice specified by each state. The hearing procedure provides procedural guarantee for the subsequent rights protection activities of acupuncturists.


Assuntos
Terapia por Acupuntura , Acupuntura , Terapia por Acupuntura/métodos , Ohio , Estados Unidos
15.
Int J Biol Macromol ; 220: 1031-1048, 2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-35961559

RESUMO

Natural products (+)-nootkatone is an important sesquiterpene compound and is widely used in pharmaceutical, cosmetic, agricultural and food industries. The aim of this study was to analyze the differentially expressed proteins (DEPs) during citrus aroma compound (+)-valencene biotransformation to (+)-nootkatone by Yarrowia lipolyticaby with high-throughput LC-MS/MS. A total of 778 proteins were differentially expressed, 385 DEPs were significantly up-regulated and 393 DEPs were markedly down-regulated. It was found that the enzymes transformed (+)-valencene to (+)-nootkatone were mainly existed in yeast intracellular and precipitated under the condition of 30-40 % ammonium sulfate. Most DEPs involved in amino acid and fatty acid metabolism were down-regulated during (+)-valencene biotransformation. The DEPs related to the carbohydrate metabolism, energy metabolism and most of transporter proteins were significantly up-regulated. Furthermore, the key enzymes involved in (+)-valencene transformation might be related to cytochrome P450s (gene2215 and gene2911) and dehydrogenases (gene6493). This is the first time that proteomics was used to investigate the metabolism mechanism of Yarrowia lipolytica during (+)-valencene biotransformation. The proteomic analysis of Yarrowia lipolytica provided a foundation for the molecular regulatory mechanism in the biotransformation to (+)-nootkatone from (+)-valencene.

16.
Neural Netw ; 154: 521-537, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35987063

RESUMO

How to obtain good retrieval performance in the case of few-shot labeled samples is the current research focus of Person Re-Identification. To facilitate formal analysis, we formally put forward the concept of Pseudo-Supervised Learning (PSL) to represent a series of research works based on label generation under few-shot condition. Through extensive investigations, we find that the main problem that needs to be solved of PSL is how we can improve the quality of pseudo-label. To solve this problem, in this work, we proposed a simple yet effective Heterogeneous Pseudo-Supervised Learning (H-PSL) framework based on classical PSL to implement asynchronous match, which boosts the feature expression and then a better label prediction in the following. Specifically, a novel isomer is constructed as the feature extractor and is trained with a much larger amount of pseudo-supervised data, i.e., samples with pseudo-labels. In this way, the isomer obtains advanced feature expression. We then deliberately implement a cross-level asynchronous match mechanism between model and pseudo-supervised data. As a result, the quality of pseudo-label is greatly improved and the feature expression performance also be optimized accordingly. In addition, to make better use of pseudo-supervised data, we also designed a knowledge fusion strategy to integrate the pseudo labels and their confidence which are easily obtained by the base model and isomer. Encouragingly, knowledge fusion strategy further removes the noise-labeled samples from candidate data. We conduct experiments on four popular datasets to fully verify the universality of the proposed method. The experimental results show that the proposed method improves the performance of all compared baseline works.

17.
Am J Transl Res ; 14(7): 5098-5106, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35958468

RESUMO

OBJECTIVE: To analyze the effect of transvaginal specimen removal on sexual function, life quality and short-term efficacy of patients diagnosed with colorectal cancer. METHOD: We retrospectively analyzed the clinical data of 100 patients with colorectal cancer treated in the Cancer Hospital Chinese Academy of Medical Sciences from September 2017 to December 2019. Among them, 52 patients who underwent transvaginal laparoscopic mid-to-high rectal cancer radical resection without assisted abdominal incision who were chosen as the observation group, and 48 patients with conventional laparoscopic-assisted mid-to-high rectal cancer radical resection were set as the control group. Intraoperative blood loss, number of lymph nodes retrieved, operation time, time of urinary catheter removal, drainage tube removal time and postoperative hospital stay were recorded and compared between the two groups. During the process, the Visual Analogue Scale (VAS) was used to assess the pain status of both groups of patients 24 hours after the operation, and the Female Sexual Function Index was used to evaluate the sexual function quality of patients before and 3 months after surgery. Postoperative complications, 2-year survival rate and 6-month postoperative quality of life of both groups of patients were evaluated and compared. RESULTS: In comparison with the control group, the amount of intraoperative blood loss, catheter removal time, drainage tube removal time, and length of postoperative hospital stay were significantly reduced in the observation group, while the number of lymph nodes retrieved was significantly greater. The VAS score 24 hours after operation was lower in the observation group compared with the control group, with statistical significance. There was no significant difference in postoperative sexual function and complication rates between the two groups. However, the 2-year survival rate and the quality of life 6 months after surgery were comparatively higher in the observation group. CONCLUSION: Natural orifice specimen extraction surgery (NOSES) is effective for transvaginal specimen removal of patients with colorectal cancer, and can significantly improve the prognosis and life quality of patients without affecting their sexual function, with a high safety profile, which is worthy of clinical promotion.

18.
Proteomics Clin Appl ; : e2100132, 2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36006834

RESUMO

PURPOSE: To develop a robust microarray platform to detect thousands of serological autoantibodies (AAbs) simultaneously in different diseases. EXPERIMENTAL DESIGN: An AAbMap microarray was prepared by printing a total of 4032 purified His-tagged human proteins and peptide probes on a chemically-modified slide. The sensitivity, dynamic range, and the inter- and intra-array reproducibility of the AAb microarray were then systematically tested and optimized. Finally, the large-scale profiling of AAbs in the serum of patients with different human diseases using the AAbMap microarray was demonstrated. RESULTS: The dynamic range of antibody (Ab) detection was 2 to 3 orders of magnitude with the lowest limit of detection (LOD) of 68 pg/mL. The intra-array (r) correlation of duplicate spots was 1.00, whereas the inter-array correlations between different arrays and batches were 0.99 and 0.97 to 0.98, respectively. Notably, 132, 266, 171, and 84 AAbs were detected in pooled serum from healthy controls (HCs) or patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), or lung cancer (LC), respectively. These AAbs included antibodies that target well-known disease biomarkers, such as anti-cyclic citrullinated peptide, anti-ribonucleoprotein, and anti-nucleosome. CONCLUSIONS AND CLINICAL RELEVANCE: We developed a microarray platform to measure thousands of serological AAbs simultaneously with high sensitivity and reproducibility. The array can help study autoimmunity and complement genomics, proteomics, and metabolomics data for systematic investigations of human diseases.

19.
iScience ; 25(9): 104870, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36034217

RESUMO

Time-restricted eating (TRE) is known to improve metabolic health, whereas very few studies have compared the effects of early and late TRE (eTRE and lTRE) on metabolic health. Overweight and obese young adults were randomized to 6-h eTRE (eating from 7 a.m. to 1 p.m.) (n = 21), 6-h lTRE (eating from 12 p.m. to 6 p.m.) (n = 20), or a control group (ad libitum intake in a day) (n = 19). After 8 weeks, 6-h eTRE and lTRE produced comparable body weight loss compared with controls. Compared with control, 6-h eTRE reduced systolic blood pressure, mean glucose, fasting insulin, insulin resistance, leptin, and thyroid axis activity, whereas lTRE only reduced leptin. These findings shed light on the promise of 6-h eTRE and lTRE for weight loss. Larger studies are needed to assess the promise of eTRE to yield better thyroid axis modulation and overall cardiometabolic health improvement.

20.
Front Artif Intell ; 5: 922589, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35795012

RESUMO

Fake reviews have become prevalent on various social networks such as e-commerce and social media platforms. As fake reviews cause a heavily negative influence on the public, timely detection and response are of great significance. To this end, effective fake review detection has become an emerging research area that attracts increasing attention from various disciplines like network science, computational social science, and data science. An important line of research in fake review detection is to utilize graph learning methods, which incorporate both the attribute features of reviews and their relationships into the detection process. To further compare these graph learning methods in this paper, we conduct a detailed survey on fake review detection. The survey presents a comprehensive taxonomy and covers advancements in three high-level categories, including fake review detection, fake reviewer detection, and fake review analysis. Different kinds of fake reviews and their corresponding examples are also summarized. Furthermore, we discuss the graph learning methods, including supervised and unsupervised learning approaches for fake review detection. Specifically, we outline the unsupervised learning approach that includes generation-based and contrast-based methods, respectively. In view of the existing problems in the current methods and data, we further discuss some challenges and open issues in this field, including the imperfect data, explainability, model efficiency, and lightweight models.

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