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1.
Artigo em Chinês | MEDLINE | ID: mdl-35511620

RESUMO

Objective:This study sought to explore the effect of age on arousal index in patients with OSAHS, and the significance of arousal index among different phenotypes identified through cluster analysis according to clinical symptoms and complications. Methods:A total of 607 adult patients with OSAHS who received polysomnography in the Second Affiliated Hospital of Xi'an Jiaotong University from July 2020 to July 2021 were selected. All patients registered basic human data, symptoms, complaints and complications, completed the Epworth Sleepiness Scale. We explored the distribution of several PSG parameters in different age groups, and included typical symptoms and complications into cluster analysis to explore the parameter differences in patients with different phenotypes. Results:Young patients had the lowest arousal index and arousal frequency in NREM stage, in middle-aged patients, the arousal index was relatively higher, the arousal times during NREM with oxygen desaturation were the highest. Among elderly patients, the wakefulness after sleep onset(WASO) was the longest, the arousal times in REM sleep was the lowest, and the spontaneous arousal times in NREM sleep were the highest(P<0.05). Among the 3 types according to the cluster analysis, phenotype Ⅰwas characterised by maximally complications, excessive daytime sleepiness and obesity, while memory and attention impairment were obvious in phenotype Ⅱ. Phenotype Ⅲhad minimal complications, relatively better mental state with shorter time-course of snoring and apnea. Phenotype I differed significantly by higher severity, more severe hypoxemia, higher arousal index and longer WASO time(P<0.05). Conclusion:the arousal index distribution varies among OSAHS patients with different age, and cluster analysis shows that patients with severe symptoms and more complications tend to have higher arousal index.


Assuntos
Distúrbios do Sono por Sonolência Excessiva , Apneia Obstrutiva do Sono , Idoso , Nível de Alerta , Humanos , Pessoa de Meia-Idade , Polissonografia , Apneia Obstrutiva do Sono/complicações , Ronco/complicações
2.
J Sens Stud ; : e12745, 2022 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-35601744

RESUMO

Cultural differences have been reported between the taste sensitivity of persons of Asian and European ancestry, although findings have been mixed. This study sought to determine whether American and Chinese adults perform differently on a novel taste test that requires no water, can be self-administered, and employs a representative of umami as one of its tastants. This 53-trial test was administered to 113 Chinese and 214 Americans. The subjects orally sampled monomer cellulose pads containing one of four dried concentrations of sucrose, citric acid, NaCl, caffeine, and monosodium glutamate and indicated whether a sweet, sour, bitter, salty, brothy, or no taste sensation was perceived. Separate gender by culture analyses of covariance with age as the covariate were performed on the total score and the scores of each taste stimulus. For all taste qualities, women outperformed men and test scores declined with age. No difference between American and Chinese subjects was found for the total taste score (p = .129) or for the sucrose (p = .129) or NaCl (p = .368) scores. However, for monosodium glutamate, the scores were 28.40% higher for the Chinese than for the American subjects (p = .024), and for citric acid and caffeine, the scores were 24.12 and 21.79% higher for the American subjects (p's = .001 and .029). The basis for these differences is unclear, although both anatomical (e.g., differences in density or distribution of taste buds) and cultural factors may be involved. Future work is needed to determine the cause of these largely novel findings and whether they generalize to other Chinese and American samples. Practical applicationsIn this study, a practical self-administered quantitative taste test that requires no water was found to be sensitive to quality-specific differences in test scores between Chinese and American subjects, as well as to age and gender. The Chinese subjects outperformed the American subjects in correctly identifying the quality of monosodium glutamate (umami), whereas the American subjects outperformed Chinese subjects in correctly identifying the bitter and sour qualities of caffeine and citric acid, respectively. Experiential factors related to culture-specific cuisines may explain some of these differences. This research indicates that a relatively rapid taste test, which can be sent through the mail and which requires no test administrator or source of water, can be used in cross-cultural studies to elucidate individual differences in taste perception.

3.
Powder Technol ; 405: 117520, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35602760

RESUMO

The coronavirus disease 2019 (COVID-19) pandemic has led to severe social and economic disruption worldwide. Although currently no consent has been reached on a specific therapy that can treat COVID-19 effectively, several inhalation therapy strategies have been proposed to inhibit SARS-CoV-2 infection. These strategies include inhalations of antiviral drugs, anti-inflammatory drugs, and vaccines. To investigate how to enhance the therapeutic effect by increasing the delivery efficiency (DE) of the inhaled aerosolized drug particles, a patient-specific tracheobronchial (TB) tree from the trachea up to generation 6 (G6) with moderate COVID-19 symptoms was selected as a testbed for the in silico trials of targeted drug delivery to the lung regions with pneumonia alba, i.e., the severely affected lung segments (SALS). The 3D TB tree geometry was reconstructed from spiral computed tomography (CT) scanned images. The airflow field and particle trajectories were solved using a computational fluid dynamics (CFD) based Euler-Lagrange model at an inhalation flow rate of 15 L/min. Particle release maps, which record the deposition locations of the released particles, were obtained at the inlet according to the particle trajectories. Simulation results show that particles with different diameters have similar release maps for targeted delivery to SALS. Point-source aerosol release (PSAR) method can significantly enhance the DE into the SALS. A C++ program has been developed to optimize the location of the PSAR tube. The optimized simulations indicate that the PSAR approach can at least increase the DE of the SALS by a factor of 3.2× higher than conventional random-release drug-aerosol inhalation. The presence of the PSAR tube only leads to a 7.12% change in DE of the SALS. This enables the fast design of a patient-specific treatment for reginal lung diseases.

4.
J Inflamm Res ; 15: 603-612, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35115808

RESUMO

BACKGROUND: Allergic rhinitis (AR) is a common inflammatory airway disease, and allergen-specific immunotherapy (AIT) is the only disease-modifying treatment for it. However, not all AR patients respond to AIT, and early prediction of patient response is extremely important. This study aimed to example serum levels of multiple cytokines in AR and explore their association with the efficacy of AIT. METHODS: A total of 74 AR patients treated with sublingual immunotherapy (SLIT) were prospectively recruited. Serum samples were obtained before the onset of SLIT and cytokine levels detected by multiplex analysis. All patients were followed for >1 year, and associations between cytokine levels and the early efficacy of SLIT were evaluated. Significantly distinctive cytokines were further verified in another independent cohort. RESULTS: Sixty patients completed the visit schedule set: 35 patients were put into a responder group and 25 a nonresponder group. Multiple-cytokine profiling showed that cytokine levels differed significantly between the two groups. The responder group had higher concentrations of BAFF and CCL11 and lower levels of CCL2, CCL7, IFNγ, IL8, IL10, IL16, and IL33 than the nonresponder group (P<0.05). Receiver-operating characteristic curves highlighted that serum BAFF, IFNγ, IL10, and IL33 levels were strongly predictive of the efficacy of SLIT (area under the curve <0.7, P<0.05). Serum IL10 and IL33 were overexpressed in nonresponders in the validation cohort. Patients in the responder group exhibited significantly higher IL10 levels and lower IL33 post-SLIT than pre-SLIT (P<0.05), but no statistical difference was found in nonresponders (P<0.05). CONCLUSION: Our data indicated that serum multiple-cytokine profiling was associated with response to SLIT and that IL10 and IL33 might serve as novel biomarkers for early prediction of efficacy and be involved in the therapeutic mechanisms of SLIT in AR patients.

5.
Artigo em Chinês | MEDLINE | ID: mdl-35172551

RESUMO

Objective:The aim of this study is to analyze the application value of NBI endoscopy in finding the concealed primary lesions of misdiagnosis of oropharyngeal cancer. Methods:The clinical data of patients with missed oropharyngeal cancer treated in the Department of Otolaryngology Head and neck surgery, the Second Affiliated Hospital of Xi'an Jiaotong University from May 2018 to June 2021, were retrospectively studied, and the missed diagnosis was also analyzed combined with results of NBI endoscopy. Results:In 31 cases of misdiagnosis of oropharyngeal cancer patients, including 25 males and 6 females, there was no significant difference in age, BMI index, course of disease and TNM stage (P> 0.05), and the pharyngeal or cervical symptoms were the first clinical manifestations of them, containing pharyngeal pain in 17 cases(54.8%) , pharyngeal foreign body sensation in 4 cases(12.9%) and unilateral cervical mass in 10 cases (32.3%). No laryngoscopy was performed (21 cases) or no primary lesion was found by laryngoscopy (10 cases) at initial diagnosis. Among them, "inflammatory lesions" were given anti-inflammatory treatment with ineffective results or surgical resection was explored for suspicious lesions (17 cases), or imaging examination (9 cases, including 6 cases with CT and MRI, 3 cases with PET-CT) and cervical lymph node biopsy (5 cases) were carried out for further diagnosis. According to these results, they were given ordinary laryngoscope (2 cases) or NBI endoscopy (29 cases) subsequently, finally they were confirmed as oropharyngeal squamous cellcarcinoma after localized biopsy at the suspicious lesions, indicating that the accuracy of NBI endoscopy in finding the concealed primary lesions of oropharyngeal cancer (93.55%) is significantly higher than that of ordinary electronic laryngoscope (6.45%)(χ²=43.613, P<0.01). Conclusion:NBI endoscopy has unique advantages in finding oropharyngeal cancer in concealed parts such as tonsil, root of tongue, soft palate and lateral wall of oropharynx, which could reduce misdiagnosis of oropharyngeal cancer.


Assuntos
Neoplasias Orofaríngeas , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Erros de Diagnóstico , Endoscopia/métodos , Feminino , Humanos , Masculino , Imagem de Banda Estreita/métodos , Neoplasias Orofaríngeas/diagnóstico por imagem , Estudos Retrospectivos
6.
Artigo em Chinês | MEDLINE | ID: mdl-34979610

RESUMO

Objective:To investigate the clinical features of diffuse large B-cell lymphoma (DLBCL) of head and neck. Methods:A retrospective study was conducted among patients with DLBCL in the Department of otolaryngology and head and neck surgery of the Second Affiliated Hospital of Xi'an Jiaotong University from July 2011 to September 2021. The disease location, clinical manifestations, diagnosis, treatment and prognosis of DLBCL patients in head and neck were analyzed retrospectively. Results:Oropharynx(27 cases, including 25 cases in tonsil), neck(29 cases), nasopharynx and nasal cavity (7 cases)were included in 63 cases of DLBCL in head and neck. Pharyngalgia, pharyngeal foreign body sensation and dysphagia were the most common manifestations of oropharyngeal DLBCL, while nasal obstruction, runny nose and hyposmia were the initial manifestations of nasal and nasopharyngeal DLBCL.Under the NBI endoscopy, locally uplifted neoplasm with rough surface mucosa was observed in 34 cases DLBCL patients of oropharynx, nasopharynx and nasal cavity. Among them, 16 cases were covered with yellow-white and patchy pseudomembrane on the surface of the neoplasm, and 5 cases were detected with abnormal new vessels, including 3 cases of tonsils, 1 case of root of tongue, and 1 case of nasopharynx. Painless progressive lymphadenectasis was the common manifestation of DLBCL in head and neck, and the maximum diameter([21.3±6.7]mm) of neck lymph nodes in the same side of DLBCL was significantly larger than that in the opposite side([16.0±7.2]mm, P=0.009). Sixty-three cases of DLBCL in head and neck, including 27 cases of germinal center type(GCB), 33 cases of nongerminal center type(non-GCB), and 3 cases of non-specific DLBCL, were confirmed the diagnosis by needle biopsy(33 cases, 52.4%) and surgical resection(30 cases, 47.6%). The imaging features of DLBCL in head and neck were mostly showed as local soft tissue masses with uniform density and uneven enhancement, and the surrounding structures were often compressed and displaced. All the patients were treated with standard R-CHOP chemotherapy regimens, and overall survival was longer in normal LDH, and overall survival of the patients at low risk of IPI was longer than those at medium-high or high risk of IPI(PLDH=0.011, PIPI=0.022, P<0.05). Conclusion:DLBCL mainly occurs in oropharynx, especially the unilateral tonsil. When flake yellow-white pseudomembrane adhesion and abnormal neovessels on the surface of the mass are detected under endoscopy, and the ultrasound suggested multiple enlarged lymph nodes in the neck with large iplateral lymph nodes, the possibility of DLBCL should be considered. Surgical resection could be performed for diagnosis if necessary, and early diagnosis would have a better prognosis.


Assuntos
Linfoma Difuso de Grandes Células B , Protocolos de Quimioterapia Combinada Antineoplásica , Centro Germinativo , Humanos , Pescoço , Prognóstico , Estudos Retrospectivos
7.
Artigo em Chinês | MEDLINE | ID: mdl-34886597

RESUMO

Objective:To investigate the effect of proton pump inhibitor(PPI) treatment on salivary pepsin concentration in laryngopharyngeal reflux(LPR). Methods:152 patients with suspected LPR complaining non-specific symptoms such as foreign body sensation, dry throat, phlegm and other non-specific symptoms were enrolled, in the Second Affiliated Hospital of Xi'an Jiaotong University from August 2019 to December 2020. According to the scores of reflux symptom index(RSI) and reflux finding score(RFS), all the patients were divided into LPR (+) group and LPR (-) group, RSI (+) group and RSI (-) group, RFS (+) group and RFS (-) group . Patients in the LPR (+) group were reassessed after 1 month of PPI treatment. Saliva samples were collected from all the patients at initial diagnosis and follow-up diagnosis after treatment. The salivary pepsin concentration was determined by enzyme linked immunosorbent assay (ELISA). The differences of RSI, RFS scores and salivary pepsin concentrations before and after treatment were compared. Results:The median concentration of salivary pepsin in LPR (+) group was significantly higher than that in LPR (-) group, and (73.01 ng/mL vs 25.66 ng/mL, P<0.01), the median concentration of salivary pepsin in RFS (+) group were significantly higher than that in RFS (-) group(78.00 ng/mL vs 35.79 ng/mL, P<0.01) Furthermore, the median scores of RSI (11.00 vs 7.00, P<0.05) and RFS (9.00 vs 7.00, P<0.01) of LPR (+) patients notably decreased after PPI treatment for 1 month, and the salivary pepsin median concentration was memorably lower than that before treatment(53.60 ng/mL vs 46.49 ng/mL, P<0.05). Meanwhile, the scores of symptoms such as pharyngeal paraesthesia, heartburn, chest pain, stomachache, and the scores of signs such as false vocal fold, erythema or congestion, vocal fold edema, posterior commissure hypertrophy and thick endolaryngeal mucus were conspicuously lower after treatment than those before treatment(P<0.05). Conclusion:After 1 month of PPI treatment, the scores of partial symptoms and signs, and the salivary pepsin concentrations of LPR patients decreased significantly, suggesting that pepsin plays an important role in the pathogenesis of LPR, and pepsin may be closely related to the symptoms and signs such as pharyngeal paraesthesia and vocal fold edema.


Assuntos
Refluxo Laringofaríngeo , Pepsina A , Humanos , Refluxo Laringofaríngeo/tratamento farmacológico , Pepsina A/metabolismo , Estudos Prospectivos , Inibidores da Bomba de Prótons/uso terapêutico , Bombas de Próton
8.
Materials (Basel) ; 14(23)2021 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-34885534

RESUMO

Coating on the surface is one of the main ways to improve the corrosion resistance and wear resistance of materials. In this work, the corrosion, erosion, and wear resistance of WC-10Co4Cr coating and 27CrMoV substrate were compared by simulating the actual working conditions of the drill pipe. The simulation results show that the most serious corrosion occurred at the pipe body and the dominating erosion arose at the pipe joint closing to the inlet of the flow field. WC-10Co4Cr coating has excellent protection to 27CrMoV substrate, resulting in a 400 mV increase in corrosion potential, a two-orders-of-magnitude decrease in the corrosion current, and four times the improvement of the impedance value. The erosion resistance of the WC-10Co4Cr coating increased to more than 30% higher than that of the 27CrMoV substrate. The friction coefficient of the WC-10Co4Cr coating was much lower than that of the 27CrMoV substrate, and the wear resistance of the coating was higher than that of the substrate.

9.
Otol Neurotol ; 42(10): e1698-e1705, 2021 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-34766954

RESUMO

OBJECTIVE: To report the clinical characteristics and treatment outcomes, as well as endoscopic-assisted ear surgery techniques used in patients with advanced external auditory canal cholesteatoma (EACC). STUDY DESIGN: Retrospective case series. SETTING: University hospital. METHODS: From October 2014 to September 2017, adult patients (age > 18) with advanced EACC (Naim's classification: stage III or IV) who underwent transcanal endoscopic ear surgery (TEES) were enrolled. The presenting features, extent of the lesion, and reconstruction techniques used were assessed. The healing time which was defined as the time required to develop a dry, re-epithelialized, and self-cleaning external auditory canal, was compared between stage III and IV. RESULTS: Twenty-three patients were included. EACC was categorized as stage III in 11 ears and stage IV in 12 ears. Cholesteatoma involved the mastoid (30%), middle ear (26%), chorda tympani (22%), temporomandibular joint, antrum, and facial nerve (17% for each). In 96% of patients, a dry and self-cleaning external auditory canal (EAC) was maintained after a mean follow-up of 15 months. The median healing time was 8 weeks in stage III, which was significantly shorter than the 12 weeks required for stage IV (p < 0.05). There was no significant difference in the median healing time between TEES and the canal wall up mastoidectomy for stage IV EACC (14 weeks) performed by the same surgeon over the same period (p > 0.05). CONCLUSIONS: TEES is a feasible and safe technique for the exposure and eradication of advanced EACC. Some critical endoscopic techniques for resecting disease and reconstructing the defect in the EAC and middle ear should be mastered before performing this operation.


Assuntos
Colesteatoma da Orelha Média , Colesteatoma , Procedimentos Cirúrgicos Otológicos , Adulto , Colesteatoma/cirurgia , Colesteatoma da Orelha Média/cirurgia , Meato Acústico Externo/cirurgia , Humanos , Processo Mastoide/cirurgia , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Otológicos/métodos , Estudos Retrospectivos
10.
Front Neurol ; 12: 748990, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777221

RESUMO

Background: The Head Impulse Paradigm (HIMP) and Suppression Head Impulse Paradigm (SHIMP) are objective, quantitative methods that directly test the vestibulo-ocular reflex (VOR) and are increasingly becoming a standard in evaluating patients with vestibular disorders. Objective: The main objective was to assess the correlations between HIMP and SHIMP parameters in patients with superior vestibular neuritis (VN) and healthy participants. Additionally, the correlations between the parameters of each method were analyzed. Methods: A retrospective cohort, non-randomized study was designed. HIMP and SHIMP were performed on 40 patients with VN and 20 healthy participants (40 ears). HIMP and SHIMP parameters were measured and calculated. Pearson's or Spearson's correlations were used to establish the associations among them. Results: A strong positive correlation was found between HIMP and SHIMP gain (Pearson's r = 0.957, p = 0.000), while strong negative correlations were detected between HIMP and SHIMP saccade amplitudes (r = -0.637, p = 0.000) and percentages of overt saccades (r = -0.631, p = 0.000). In HIMP, strong and moderate positive correlations were identified between gain and saccade amplitude (R 2 = 0.726, p = 0.000) and gain and saccade percentage (R 2 = 0.558, p = 0.000), respectively. By contrast, an extremely weak positive correlation was observed between gain and latency (R 2 = 0.053, p = 0.040). In SHIMP, strong and moderate positive correlations were found between gain and saccade percentage (R 2 = 0.723, p = 0.000) and gain and saccade amplitude (R 2 = 0.525, p = 0.000), respectively, but no correlation was detected between gain and latency (R 2 = 0.006, p = 0.490). Conclusions: HIMP and SHIMP-related parameters were highly correlated (inter-method). Within each method (intra-method), moderate to strong correlations in VOR assessment were observed. These results further contribute to our understanding of the relationship between HIMP and SHIMP as well as to the diagnosis.

11.
Int J Pediatr Otorhinolaryngol ; 150: 110901, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34450546

RESUMO

OBJECTIVE: To explore the relationship between obstructive sleep apnea (OSA) and cognitive impairment by combining event-related evoked potentials (ERPs) and China-Wechsler Younger Children Scale (C-WISC) in children with sleep-disordered breathing (SDB) with vs. without OSA. METHODS: This was a retrospective case-control study of all consecutive children (n = 148) with adenoid tonsil hypertrophy between July 2017 and March 2019 at the Hospital. RESULTS: The children were divided into the OSA (n = 102) and non-OSA (n = 46) groups. The apnea-hypopnea index (AHI), obstructive apnea index (OAI), and obstructive apnea-hypopnea index (OAHI) in the OSA group were elevated compared with those of the non-OSA group (all P < 0.001). The mean oxygen saturation (SaO2) and SaO2 nadir were lower in the OSA group compared with the non-OSA group (both P < 0.001). The respiratory arousal index (RAI) values in the OSA group were larger than those of the non-OSA group (P < 0.001). The P300 and N100 latencies in the OSA group were longer than those of the non-OSA group (both P < 0.001). Pearson's correlation analysis revealed correlations of the P300 peak latency with full-scale intelligence quotient (FIQ) (P < 0.001 and r = -0.527), verbal intelligence quotient (VIQ) (P < 0.001 and r = -0.448), and performance intelligence quotient (PIQ) (P < 0.001 and r = -0.515). There was a correlation between the N100 peak latency and PIQ (P = 0.026 and r = -0.183). CONCLUSION: ERPs, as an objective measurement, might help assess cognitive impairment in children with OSA.


Assuntos
Disfunção Cognitiva , Apneia Obstrutiva do Sono , Estudos de Casos e Controles , Criança , China , Disfunção Cognitiva/diagnóstico , Disfunção Cognitiva/etiologia , Potenciais Evocados , Humanos , Polissonografia , Estudos Retrospectivos , Apneia Obstrutiva do Sono/complicações , Apneia Obstrutiva do Sono/diagnóstico , Escalas de Wechsler
12.
Mediators Inflamm ; 2021: 5576596, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34194284

RESUMO

BACKGROUND: Suppressor of tumorigenicity 2 (ST2) is a key biomarker in inflammation and cardiovascular diseases, but limited data is available on its role in allergic rhinitis (AR). OBJECTIVE: The aim of this study is to explore the role of serum soluble ST2 (sST2) in evaluating disease severity and predicting the efficacy of sublingual immunotherapy (SLIT) in house dust mite- (HDM-) induced AR patients. METHODS: Eighty healthy controls (HC group) and 160 HDM-induced AR patients, including 40 mild patients (MAR group) and 120 moderate-severe patients (MSAR group), were recruited in this study. Serum was collected from all participants and levels of sST2 were determined by ELISA and the relationship between sST2 levels and disease severity was assessed. In the MSAR group, 109 patients received 3 years of SLIT, and the relationship between serum levels of sST2 and efficacy of SLIT was exampled. RESULTS: Serum sST2 levels were increased in HDM-induced AR patients compared to the HC group (P < 0.001), and the concentrations were higher in the MSAR group than in the MAR group and HC group (all P < 0.05). Moreover, sST2 levels positively correlated with the total nasal symptom score (TNSS), visual analogue scale (VAS), and specific IgE levels (P < 0.05). Seventy-eight MSAR patients accomplished SLIT, and they were divided into an effective group (n = 40) and an ineffective group (n = 38). The serum sST2 levels in the effective group were lower than those in the ineffective group (P < 0.001). In addition, patients in the effective group levels exhibited significantly lower sST2 levels post-SLIT than pre-SLIT (P < 0.001), but no statistic difference was observed in the ineffective group (P > 0.05). Receiver operating characteristic (ROC) curve showed promising accuracy for predicting clinical efficacy of SLIT in AR patients (area under the curve = 0.839, P < 0.001). CONCLUSION: Serum sST2 is a potential biomarker for assessing disease severity and may serve as a sensitive biomarker for predicting the therapeutic response of SLIT in HDM-induced AR patients.


Assuntos
Biomarcadores/metabolismo , Imunoterapia/métodos , Proteína 1 Semelhante a Receptor de Interleucina-1/sangue , Rinite Alérgica/sangue , Rinite Alérgica/imunologia , Imunoterapia Sublingual/métodos , Administração Sublingual , Adulto , Alérgenos , Animais , Doenças Cardiovasculares/sangue , Estudos de Casos e Controles , Feminino , Humanos , Inflamação , Masculino , Estudos Prospectivos , Pyroglyphidae , Curva ROC , Sensibilidade e Especificidade
13.
Cancer Prev Res (Phila) ; 14(8): 767-778, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34021022

RESUMO

Although the principle of systemic treatment to prevent the progression of oral premalignant lesions (OPL) has been demonstrated, there remains a lack of consensus about an optimal approach that balances clinical efficacy with toxicity concerns. Recent advances in cancer therapy using approaches targeting the tumor immune microenvironment (TIME) including immune-checkpoint inhibitors indicate that these agents have significant clinically activity against different types of cancers, including oral cancer, and therefore they may provide an effective oral cancer prevention strategy for patients with OPLs. Our past work showed that systemic delivery of a monoclonal antibody to the programmed death receptor 1 (PD-1) immune checkpoint can inhibit the progression of OPLs to oral cancer in a syngeneic murine oral carcinogenesis model. Here we report a novel approach of local delivery of a PD-1 immune-checkpoint inhibitor loaded using a hydrogel, which significantly reduces the progression of OPLs to carcinomas. In addition, we detected a significant infiltration of regulatory T cells associated with oral lesions with p53 mutation, and a severe loss of expression of STING, which correlated with a decreased infiltration of dendritic cells in the oral lesions. However, a single local dose of PD-1 inhibitor was found to restore stimulator of interferon response cGAMP interactor 1 (STING) and CD11c expression and increase the infiltration of CD8+ T cells into the TIME irrespective of the p53 mutational status. Overall, we provide evidence for the potential clinical value of local delivery of biomaterials loaded with anti-PD-1 antibodies to prevent malignant progression of OPLs. PREVENTION RELEVANCE: Oral cancer is an aggressive disease, with an overall survival rate of 50%. Preinvasive histologic abnormalities such as tongue dysplasia represent an early stage of oral cancer; however, there are no treatments to prevent oral carcinoma progression. Here, we combined biomaterials loaded with an immunotherapeutic agent preventing oral cancer progression.


Assuntos
Inibidores de Checkpoint Imunológico/administração & dosagem , Neoplasias Bucais/prevenção & controle , Lesões Pré-Cancerosas/tratamento farmacológico , Receptor de Morte Celular Programada 1/imunologia , 4-Nitroquinolina-1-Óxido , Animais , Anticorpos Monoclonais/administração & dosagem , Carcinogênese/induzido quimicamente , Carcinogênese/efeitos dos fármacos , Modelos Animais de Doenças , Sistemas de Liberação de Medicamentos , Feminino , Genes p53/genética , Neoplasias de Cabeça e Pescoço/induzido quimicamente , Neoplasias de Cabeça e Pescoço/genética , Neoplasias de Cabeça e Pescoço/patologia , Neoplasias de Cabeça e Pescoço/prevenção & controle , Humanos , Injeções Intralesionais , Masculino , Camundongos , Camundongos Transgênicos , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/induzido quimicamente , Lesões Pré-Cancerosas/genética , Lesões Pré-Cancerosas/patologia , Receptor de Morte Celular Programada 1/antagonistas & inibidores , Quinolonas , Carcinoma de Células Escamosas de Cabeça e Pescoço/induzido quimicamente , Carcinoma de Células Escamosas de Cabeça e Pescoço/genética , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/prevenção & controle
14.
Materials (Basel) ; 14(6)2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33803508

RESUMO

The effects of titanium (Ti) ion-implanted doses on the chemical composition, surface roughness, mechanical properties, as well as tribological properties of 316L austenitic stainless steel are investigated in this paper. The Ti ion implantations were carried out at an energy of 40 kV and at 2 mA for different doses of 3.0 × 1016, 1.0 × 1017, 1.0 × 1018, and 1.7 × 1018 ions/cm2. The results showed that a new phase (Cr2Ti) was detected, and the concentrations of Ti and C increased obviously when the dose exceeded 1.0 × 1017 ions/cm2. The surface roughness can be significantly reduced after Ti ion implantation. The nano-hardness increased from 3.44 to 5.21 GPa at a Ti ion-implanted dose increase up to 1.0 × 1018 ions/cm2. The friction coefficient decreased from 0.78 for un-implanted samples to 0.68 for a sample at the dose of 1.7 × 1018 ions/cm2. The wear rate was slightly improved when the sample implanted Ti ion at a dose of 1.0 × 1018 ions/cm2. Adhesive wear and oxidation wear are the main wear mechanisms, and a slightly abrasive wear is observed during sliding. Oxidation wear was improved significantly as the implantation dose increased.

15.
Medicine (Baltimore) ; 100(12): e25154, 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33761685

RESUMO

ABSTRACT: During last decade, bioinformatics analysis has provided an effective way to study the relationship between various genes and biological processes. In this study, we aimed to identify potential core candidate genes and underlying mechanisms of progression of lung and gastric carcinomas which both originated from endoderm. The expression profiles, GSE54129 (gastric carcinoma) and GSE27262 (lung carcinoma), were collected from GEO database. One hundred eleven patients with gastric carcinoma and 21 health people were included in this research. Meanwhile, there were 25 lung carcinoma patients. Then, 75 differentially expressed genes were selected via GEO2R online tool and Venn software, including 31 up-regulated genes and 44 down-regulated genes. Next, we used Database for Annotation, Visualization, and Integrated Discovery and Metascpe software to analyze Kyoto Encyclopedia of Gene and Genome pathway and gene ontology. Furthermore, Cytoscape software and MCODE App were performed to construct complex of these differentially expressed genes . Twenty core genes were identified, which mainly enriched in extracellular matrix-receptor interaction, focal adhesion, and PI3K-Akt pathway (P < .01). Finally, the significant difference of gene expression between cancer tissues and normal tissues in both lung and gastric carcinomas was examined by Gene Expression Profiling Interactive Analysis database. Twelve candidate genes with positive statistical significance (P < .01), COMP CTHRC1 COL1A1 SPP1 COL11A1 COL10A1 CXCL13 CLDN3 CLDN1 matrix metalloproteinases 7 ADAM12 PLAU, were picked out to further analysis. The Kaplan-Meier plotter website was applied to examine relationship among these genes and clinical outcomes. We found 4 genes (ADAM12, SPP1, COL1A1, COL11A1) were significantly associated with poor prognosis in both lung and gastric carcinoma patients (P  < .05). In conclusion, these candidate genes may be potential therapeutic targets for cancer treatment.


Assuntos
Biomarcadores Tumorais/genética , Biologia Computacional , Neoplasias Pulmonares/genética , Neoplasias Gástricas/genética , Proteína ADAM12/genética , Colágeno Tipo I/genética , Colágeno Tipo XI/genética , Bases de Dados Genéticas , Perfilação da Expressão Gênica , Humanos , Estimativa de Kaplan-Meier , Análise em Microsséries , Osteopontina/genética , Prognóstico , Mapas de Interação de Proteínas
16.
Oncol Rep ; 44(6): 2503-2516, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33125127

RESUMO

Aberrant methylation is one of the most frequent epigenetic alterations that regulate the expression levels of genes, including long non­coding RNAs (lncRNAs), in tumors. However, to the best of our knowledge, the expression and function of hepatic nuclear factor 1α antisense RNA 1 (HNF1A­AS1) and its methylation condition have not yet been reported in the development and progression of laryngeal squamous cell carcinoma (LSCC). In the present study, the expression and methylation of HNF1A­AS1 were first examined by reverse transcription­quantitative PCR, bisulfite genomic sequencing and methylation­specific polymerase chain reaction in samples from patients with LSCC, which were based on the in silico analysis using The Cancer Genome Atlas data, and were then further verified in LSCC cell lines with and without 5­Aza­2'­deoxycytidine (5­Aza­dC) treatment. Subsequently, proliferation, cell cycle distribution, migration and invasion of LSCC cells following either knockdown or overexpression of HNF1A­AS1 were determined in vitro. Furthermore, the characteristic of HNF1A­AS1 on epithelial­mesenchymal transition (EMT) changes was investigated in vitro and in vivo. The associations between the expression levels of HNF1A­AS1 and tumorigenicity and cervical lymph node metastasis were assessed in a xenograft model in nude mice. In the present study, downregulation and hypermethylation in CpG sites of HNF1A­AS1 were detected in LSCC tissues as well as metastatic cervical lymph nodes samples when compared with those in the adjacent non­tumor tissues. Additionally, HNF1A­AS1 inhibited proliferation, migration and invasion of LSCC cells in vitro by regulating the process of EMT. Furthermore, HNF1A­AS1 inhibited tumor growth and metastasis by regulating EMT in vivo. Additionally, the migration and invasion abilities, and the expression levels of HNF1A­AS1 and EMT markers in LSCC cells were significantly reversed by treatment with 5­Aza­dC. In summary, HNF1A­AS1 was downregulated by hypermethylation in LSCC and laryngeal cancer cells. These findings suggested that HNF1A­AS1 could serve as a tumor suppressor lncRNA in LSCC by regulating the EMT process, leading to the discovery of novel therapeutic targets and strategies for the treatment of patients with LSCC.


Assuntos
Transição Epitelial-Mesenquimal/genética , Neoplasias Laríngeas/genética , Metástase Linfática/genética , RNA Longo não Codificante/genética , Carcinoma de Células Escamosas de Cabeça e Pescoço/genética , Idoso , Animais , Azacitidina/farmacologia , Azacitidina/uso terapêutico , Linhagem Celular Tumoral , Quimioterapia Adjuvante/métodos , Ilhas de CpG , Metilação de DNA/efeitos dos fármacos , Progressão da Doença , Regulação para Baixo , Epigênese Genética/efeitos dos fármacos , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Técnicas de Silenciamento de Genes , Humanos , Neoplasias Laríngeas/diagnóstico , Neoplasias Laríngeas/patologia , Neoplasias Laríngeas/terapia , Laringectomia , Laringe/cirurgia , Metástase Linfática/patologia , Masculino , Camundongos , Pessoa de Meia-Idade , RNA Longo não Codificante/metabolismo , Carcinoma de Células Escamosas de Cabeça e Pescoço/diagnóstico , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/terapia , Ensaios Antitumorais Modelo de Xenoenxerto
17.
Oncol Rep ; 44(5): 2080-2092, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33000261

RESUMO

Emerging studies have demonstrated that long non­coding RNAs (lncRNAs) play essential roles in tumorigenesis. However, the role and function of lncRNAs in hypopharyngeal squamous cell carcinoma (HSCC) have not been completely elucidated. The present study explored the function of a novel lncRNA, RP11­156L14.1, in HSCC. RP11­156L14.1 was revealed to be highly expressed in HSCC tissues and cell lines. Knockdown of RP11­156L14.1 inhibited proliferation, migration, and invasion in HSCC cells. Furthermore, RP11­156L14.1 regulated epithelial­mesenchymal transition (EMT) by controlling EMT­related protein expression. Mechanistically, RP11­156L14.1 exerted its function as a competing endogenous RNA (ceRNA) and directly interacted with miR­548ao­3p. The present study also demonstrated that miR­548ao­3p regulated signal sequence receptor subunit 1 (SSR1) expression by targeting SSR1 3'­UTR. Moreover, the xenograft HSCC tumor model revealed that knockdown of RP11­156L14.1 markedly suppressed HSCC tumor growth in vivo. In summary, these findings indicated that the lncRNA RP11­156L14.1 functions as an oncogene in HSCC by competing with miR­548ao­3p in regulating SSR1 expression. The RP11­156L14.1/miR­548ao­3p/SSR1 axis could be utilized as a potential novel biomarker and therapeutic target for HSCC.


Assuntos
Proteínas de Ligação ao Cálcio/genética , Neoplasias Hipofaríngeas/genética , Glicoproteínas de Membrana/genética , MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Receptores Citoplasmáticos e Nucleares/genética , Receptores de Peptídeos/genética , Carcinoma de Células Escamosas de Cabeça e Pescoço/genética , Adulto , Idoso , Animais , Carcinogênese/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Neoplasias Hipofaríngeas/patologia , Neoplasias Hipofaríngeas/cirurgia , Hipofaringe/patologia , Hipofaringe/cirurgia , Laringectomia , Masculino , Camundongos , Pessoa de Meia-Idade , Prognóstico , RNA Longo não Codificante/genética , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/cirurgia , Ensaios Antitumorais Modelo de Xenoenxerto
18.
J Int Med Res ; 48(9): 300060520957536, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32967513

RESUMO

OBJECTIVE: To determine the effect of personalized psychological interventions on the recovery of patients with sudden deafness. METHODS: Participants were 438 patients with sudden deafness who were randomly allocated to observation and control groups. Patients in the control group received routine nursing care and drug treatment. Patients in the observation group received personalized psychological interventions, routine nursing care and drug treatment. The Self-Rating Anxiety Scale (SAS) and the Self-Rating Depression Scale (SDS) were used to measure anxiety and depression. The Simple Coping Style Questionnaire (SCSQ) and the Satisfaction With Life Scale (SWLS) were used to evaluate coping styles, attitude toward life and quality of life. RESULTS: Patients in both groups had similar SAS and SDS scores at the time of admission. After treatment, there was a significant difference in SAS and SDS scores between the observation and control groups, indicating that the observation group had less anxiety and depression. Both groups had increased SCSQ and SWLS scores following treatment, indicating improvements in positive coping and satisfaction with life. CONCLUSION: Effective psychological interventions may change negative thoughts, increase response rate and improve quality of life.


Assuntos
Perda Auditiva Súbita , Qualidade de Vida , Ansiedade , Depressão , Perda Auditiva Súbita/terapia , Humanos , Intervenção Psicossocial
19.
Artigo em Chinês | MEDLINE | ID: mdl-32791598

RESUMO

Objective:To explore the main influencing factors of excessive daytime sleepiness(EDS) in adults with different degrees of sleep-disordered breathing(SDB), which will provide the scientific evidences for the individualized diagnosis and treatment. Method:Retrospective analysis was performed on the clinical data of 361 young and middle-aged snoring patients monitored by polysomnography. According to the presence or absence of obstructive sleep apnea(OSA) and apnea hypopnea index(AHI) levels, they were divided into primary snoring(AHI<5), mild OSA(AHI 5-<15), moderate OSA(AHI 15-<30) and severe OSA(AHI≥30). From sleep efficiency(ES), different sleep stages(REM, NREM1, NREM2, NREM3) ratio of total sleep, oxygen reduction index(ODI), blood oxygen saturation<90% of total sleep time(TS90%), the average saturation of blood oxygen(MSaO2) and the lowest blood oxygen saturation(LSaO2) of all-night sleep, and AHI in different sleep stages(REM-AHI, NREM-AHI), MSaO2 in different sleep stages(REM-MSaO2, NREM-MSaO2) and LSaO2 in different sleep stages(REM-LSaO2, NREM-LSaO2), the main influencing factors of EDS were discussed. Result:Among the 361 patients, 23 patients suffered from the primary snoring, 47 patients with mild OSA, 56 patients with moderate OSA, and 235 patients with severe OSA. REM-AHI, ODI and TS90% in EDS group were 10.9, 9.6 and 0.2 respectively in patients with primary snoring, which were significantly higher than those without EDS(P<0.01). The main influencing factors of EDS were REM-AHI, ODI, and TS90%. However, among patients with mild OSA, REM-AHI was the main influencing factor of EDS, REM-AHI of the EDS group was 29.6, which was significantly higher than that of the non-EDS(P<0.05). In patients with moderate OSA, LSaO2 of the EDS group was 76.2, significantly lower than the group without EDS(P<0.05), the main influencing factor of EDS is LSaO2. In patients with severe OSA, BMI, ODI and TS90% in EDS group were significantly higher than those without EDS(P<0.05 or P<0.01), while NREM-MSaO2, MSaO2 and LSaO2 were significantly lower than those without EDS(P<0.05 or P<0.01). The main influencing factors of EDS were BMI, NREM-MSaO2, MSaO2, LSaO2, ODI and TS90%. Conclusion:Frequent apnea during REM stage may be one of the important factors causing EDS in patients with primary snoring and mild OSA. For patients with moderate and severe OSA, intermittent hypoxia at night may be the main factor leading to EDS, and obesity may promote the development of the disease and the occurrence of sleepiness.


Assuntos
Síndromes da Apneia do Sono , Apneia Obstrutiva do Sono , Adulto , Humanos , Pessoa de Meia-Idade , Polissonografia , Estudos Retrospectivos , Ronco
20.
Physiol Meas ; 41(7): 075009, 2020 08 11.
Artigo em Inglês | MEDLINE | ID: mdl-32559754

RESUMO

OBJECTIVE: Polysomnography is typically used to evaluate the severity of obstructive sleep apnea (OSA) but the inconvenience of application and high cost considerably affect the diagnostics. In this study, sleep sound signals are used to detect OSA in patients. APPROACH: A deep feature transfer-based OSA detection approach is proposed. First, a deep convolutional neural network is trained on large-scale labeled audio data sets to distinguish respiration sounds from environmental noise. Second, the trained model is transferred to recognize respiration sounds in sleep sound signals. Third, the deep features of the detected respiration sounds are used to train a logistic regression classifier to identify OSA patients from potential patients. Polysomnography-based diagnosis is used as a reference. MAIN RESULTS: A self-collected data set of 132 potential OSA patients is applied in OSA detection experiments. The OSA detection performances are tested on four models for different apnea-hypopnea index thresholds and sexes resulting in accuracies of 80.17%, 80.21%, 81.63% and 77.22%. The corresponding areas under the receiver operating characteristic curves are 0.82, 0.80, 0.81 and 0.79. In addition, the proposed method presented a significant performance improvement compared with the state-of-the-art methods. SIGNIFICANCE: Big data, deep learning and transfer learning can be successfully applied to improve diagnostic accuracy in OSA detection. The performance of the proposed approach is superior to that of traditional audio analysis technology. The proposed method significantly reduces difficulties in OSA detection and diagnosis, such that potential OSA patients can perform initial inspections by themselves at home.


Assuntos
Sons Respiratórios , Processamento de Sinais Assistido por Computador , Apneia Obstrutiva do Sono , Humanos , Redes Neurais de Computação , Polissonografia , Sono , Apneia Obstrutiva do Sono/diagnóstico
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