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1.
BMC Microbiol ; 20(1): 75, 2020 04 03.
Artigo em Inglês | MEDLINE | ID: mdl-32245419

RESUMO

BACKGROUND: Oral microbiota is not only important for maintaining oral health but also plays a role in various oral diseases. However, studies regarding microbiome changes in oral lichen planus (OLP) are very limited. To the best of our knowledge, there has been only two studies investigating salivary microbiome changes in OLP. Therefore, the purpose of this study was to identify the characteristic microbial profile in the saliva of OLP patients, with or without erosive lesions, and compare that with recurrent aphthous ulcer (RAU), a common oral immunological disorder that also shows multiple erosive/ulcerative lesions. Whole saliva samples were collected from 20 patients with OLP (erosive E, n = 10 and non-erosive NE, n = 10), 10 patients with RAU (U) and 10 healthy controls (C). DNA was extracted from the saliva samples, and the 16S rDNA gene V4 hypervariable region was analyzed using Illumina sequencing. RESULTS: We obtained 4949 operational taxonomic units (OTUs) from the V4 region in all saliva samples. Community composition analysis showed a clear decreased relative abundance of genera Streptococcus and Sphingomonas in saliva from RAU patients when compared to the other three groups. Relative abundance of Lautropia and Gemella were higher in E group, whereas relative abundance of Haemophilus and Neisseria were higher in NE group when compared to C group. Abiotrophia and Oribacterium were higher in OLP (combining E and NE groups), while Eikenella and Aggregatibacter were lower when compared to C group. There was statistically significance in α-diversity between E and RAU groups(p < 0.05). Significant differences in ß-diversity were detected in bacteria between E and C; NE and C; as well as E and NE groups. The LDA effect size algorithm identified the g_Haemophilus might be the potential biomarker in NE group. CONCLUSIONS: We found that salivary microbiome in erosive OLP was significantly different from that found in RAU; and these changes may be related to the underlying disease process rather than presence of ulcerative/erosive lesions clinically. In addition, our findings in bacterial relative abundance in OLP were significantly different from the previously reported findings, which points to the need for further research in salivary microbiome of OLP.


Assuntos
Bactérias/classificação , Disbiose/microbiologia , Líquen Plano Bucal/microbiologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA/métodos , Estomatite Aftosa/diagnóstico , Algoritmos , Bactérias/genética , Bactérias/isolamento & purificação , Estudos de Casos e Controles , DNA Bacteriano/genética , DNA Ribossômico/genética , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Filogenia , Saliva/microbiologia
2.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(6): 589-593, 2019 Dec 01.
Artigo em Chinês | MEDLINE | ID: mdl-31875435

RESUMO

OBJECTIVE: To study the effect of periodontal non-surgical treatment on serum interleukin-6 (IL-6) expression and carotid vascular wall in a rat model of chronic periodontitis (CP) with type 2 diabetes mellitus (T2DM). METHODS: Twenty-eight 6-week-old SD rats were randomly divided into group A (control group, 7 rats) and group B (T2DM+CP group, 21 rats). According to different intervention measures, group B was randomly subdivided into B1 (natural process), B2 (periodontal mechanical treatment), and B3 (periodontal mechanical treatment + 2% minocycline hydrochloride + systemic metronidazole plus amoxicillin). Serum IL-6 was measured by enzyme-linked immunosorbent assay, and carotid tissue was observed under light microscopy after hematoxylin-eosin (HE) staining. RESULTS: The carotid artery wall of group A was normal. In group B1, the endothelial cells disappeared, the elastic fibers of the middle membrane were disordered, the local necrosis of smooth muscle tissue was amorphous particles, a small amount of calcium salt was deposited, and the wall thickening was obvious. The endothelial cells in groups B2 and B3 disappeared, and the smooth muscle cells in the middle membrane underwent denaturation. No significant thickening of the vessel wall was noted. The serum IL-6 level in group B1 continually increased with time, and it was higher than that in group A (P<0.001). The IL-6 levels of groups B2 and B3 peaked a week after the first intervention. Subsequently, IL-6 levels gradually decreased. At the last point (five weeks after the second intervention ), the IL-6 levels of groups B2 and B3 were significantly lower than that of group B1 (P<0.001). CONCLUSIONS: For rats with periodontitis and diabetes, periodontal mechanical treatment may elevate serum IL-6 levels in the short term but might reduce the whole inflammatory state in the long term. Thus, this treatment might be valuable to the improvement of vascular disease. The adjunctive benefits of systemic metronidazole and amoxicillin in non-surgical periodontal therapy are decreased serum IL-6 expression and normal carotid artery.


Assuntos
Periodontite Crônica , Diabetes Mellitus Tipo 2 , Animais , Artérias Carótidas , Células Endoteliais , Interleucina-6 , Ratos , Ratos Sprague-Dawley
3.
Chin J Dent Res ; 22(3): 203-209, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31508609

RESUMO

OBJECTIVE: To investigate the effects of nonsurgical periodontal therapy on the serum interleukin 6 (IL-6) and high-sensitivity C-reactive protein (hsCRP) and on the carotid artery in rats with chronic periodontitis (CP) and with or without hyperlipidemia (HL). METHODS: A total of 29 Sprague-Dawley rats were randomly divided into two groups: group A (CP, n = 14) and B (CP + HL, n = 15), and subjected to the corresponding treatment. Groups A and B were further divided into groups A1/B1 (without periodontal interventions) and A2/B2 (with periodontal interventions). The serum IL-6 and hsCRP levels were evaluated before periodontal intervention and at 1, 3, 5, and 7 weeks after periodontal intervention. The rats were euthanised 8 weeks after the periodontal intervention and the histopathologic changes in the carotid artery were observed. RESULTS: The serum hsCRP and serum IL-6 levels in groups A1 and B1 were elevated with time; they were significantly higher in group B1 than in group A1 (P < 0.001) at all time points. The hsCRP and IL-6 levels in groups A2 and B2 increased with time and reached the maximum level 1 week after the second intervention, and then gradually decreased. Atherosclerotic plaques, fibrous cap, and calcium salt deposits were apparent in the rats of group B1, whereas no obvious atherosclerotic changes were observed in the rats of groups A2 and B2. CONCLUSION: Periodontal interventions resulted in acute, short-term systemic inflammation. However, it was beneficial in long-term as it improved the carotid artery integrity.


Assuntos
Periodontite Crônica , Hiperlipidemias , Animais , Proteína C-Reativa , Artérias Carótidas , Interleucina-6 , Ratos , Ratos Sprague-Dawley
4.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(6): 1771-4, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-30052389

RESUMO

Ethanol concentration quantitative analysis of ethanol-water solution can be realized by measuring the ratio of Raman characteristic peak heights. The content of ethanol can be determined by linear relation between relative intensity ratio and ethanol concentration. However, this analytical method only applies to the ethanol solution at low concentration. Concerning this issue, relative intensity of characteristic peak of ethanol (asymmetric stretching vibration of CH2 2 924.0 cm-1) and peak background of water (3 350 cm-1) at different ethanol concentration is experimentally measured by using a self-developed laser Raman ethanol content detection system. According to the relationship between relative ratio of characteristic peak heights and ethanol concentration, the nonlinear regression analysis methods are proposed to apply in the measurement of ethanol concentration in a wide range. Adjacent region average method is utilized to remove mutational random noise in Raman spectra of ethanol solution. Combined with multi-point interpolation processing, the baseline of Raman spectra can be calibrated. The influences of mutational random noise and the strong fluorescence background can be effectively eliminated with baseline correction and normalization methods. Polynomial and exponential mathematical models are adopted for nonlinear regression analyses by the relation between ratio of characteristic peak heights and concentration of ethanol solution. The analysis results show that the correlation coefficient of linear fitting and nonlinear fitting is about 0.991 and higher than 0.997 respectively. The linear analytical method can be effectively applied when ethanol concentration range is 15%~60%. The nonlinear analytical method has higher measurement accuracy in a wider ethanol concentration range of 3%~97%. Nonlinear mathematical model will provide theoretical basis for analysis of ethanol concentration, which can be applied in laser Raman ethanol content detection system to calculate the relatively accurate ethanol concentration of ethanol-water solution. Rapid, real-time and accurate quantitative analysis of wide concentration range ethanol solution, which has mutational random noise and strong fluorescence background interference, can be achieved by these analytical methods.

5.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 47(2): 75-80, 2012 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-22490241

RESUMO

OBJECTIVE: To investigate the potential genetic mode of aggressive periodontitis (AgP) in Chinese Han nationality. METHODS: A total of 233 subjects from 73 nuclear families were recruited. All probands were diagnosed according to the criteria of AgP in 1999 classification of periodontal diseases. Ninety parents, 35 siblings and three grandparents and two offspring were examined based on full-mouth periodontal chartings (including parameter of probing depths, attachment loss, bleeding on probing at six sites per tooth) and full-mouth periapical radiographs. The genetic ratio was calculated and analyzed by the methods of Edwards and simple segregation. RESULTS: The prevalence of AgP in probands' siblings was close to the square root of the prevalence of general population. The segregation ratio was 0.2419, which was close to the theoretical ratio for autosomal recessive inheritance. However, autosomal dominant inheritance could not be rejected in families whose parent(s) suffered from severe chronic periodontitis. CONCLUSIONS: The genetic heterogeneity of AgP existed in Chinese Han nationality. The genetic mode was autosomal recessive inheritance in general, and autosomal dominant inheritance could not be excluded in families whose parent(s) suffered from severe chronical periodontitis. The results imply the genetic heterogeneity of AgP, and further demonstrate that AgP was a multifactorial disease with major genetic component in the disease etiology.


Assuntos
Periodontite Agressiva/genética , Genes Dominantes , Genes Recessivos , Heterogeneidade Genética , Periodontite Agressiva/epidemiologia , Periodontite Crônica/epidemiologia , Periodontite Crônica/genética , Feminino , Humanos , Masculino , Linhagem , Prevalência , Inquéritos e Questionários
6.
Beijing Da Xue Xue Bao Yi Xue Ban ; 42(1): 28-32, 2010 Feb 18.
Artigo em Chinês | MEDLINE | ID: mdl-20140038

RESUMO

OBJECTIVE: To analyze the correlation between polymorphisms of the vitamin D receptor gene (Taq I and Fok I) and the aggressive periodontitis by FBAT method. METHODS: 93 AgP nuclear families including 93 probands and their 155 relatives were recruited. The genotype frequency and polymorphism for VDR for the patients and their pedigree were detected by using polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP). RESULTS: The frequency of allele T and t accounted for 94.6 % and 5.4 % in the total populations. No tt genotype were detected. The fathers of probands carried more allele t than the mothers(9.8% vs 1.6%, P=0.005). The frequency of allele F and f accounted for 57.1 % and 42.9 % in the total populations. The result of family based associated test (FBAT) including additive model, dominant model and recessive model showed that different alleles of Taq I and Fok I had no correlation with the onset of AgP(P>0.05). CONCLUSION: This was the first time to detect the VDR gene polymorphisms in Chinese families. The result of FBAT analysis can not show the correlation between vitamin D receptor gene polymorphisms (Taq I and Fok I) and the onset of AgP.


Assuntos
Periodontite Agressiva/genética , Polimorfismo Genético , Receptores de Calcitriol/genética , Desoxirribonucleases de Sítio Específico do Tipo II/genética , Feminino , Frequência do Gene , Predisposição Genética para Doença/genética , Humanos , Masculino , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Taq Polimerase/genética
7.
Zhonghua Gan Zang Bing Za Zhi ; 16(12): 926-9, 2008 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-19105939

RESUMO

OBJECTIVE: To assess the value of color Doppler flow imaging (CDFI) in monitoring vascular complications following orthotopic liver transplantation (OLT). METHODS: Seven hundred ninety-two patients who received OLT from April 2002 to December 2006 in the Organ Transplantation Center, General Hospital of Chinese People's Armed Police Forces, Beijing, and underwent CDFI examinations in different periods after OLT were enrolled in this study. Their vascular complications were monitored by CDFI and confirmed by angiography or spiral CT. RESULTS: Of the 792 patients, 54 were diagnosed with vascular complications that occurred 1-360 days after their OLT operations. These complications occurred within 1-30 days, 31-60 days, 61-90 days, 91-180 days, 181-360 days, with the proportions of 46.30%, 22.22%, 14.81%, 9.26% and 7.41% respectively. The proportion of hepatic artery and portal vein complications and outflow occlusions were 61.11%, 35.19% and 3.70% respectively. CONCLUSION: Most vascular complications occurred within six months after the OLT operation. The continuous and careful monitoring by CDFI is beneficial in an early diagnosis of vascular complications after OLT.


Assuntos
Transplante de Fígado/efeitos adversos , Complicações Pós-Operatórias , Ultrassonografia Doppler em Cores/métodos , Doenças Vasculares/diagnóstico por imagem , Doenças Vasculares/etiologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Seguimentos , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Adulto Jovem
8.
Beijing Da Xue Xue Bao Yi Xue Ban ; 40(1): 28-33, 2008 Feb 18.
Artigo em Chinês | MEDLINE | ID: mdl-18278133

RESUMO

OBJECTIVE: To study whether interleukin-1( IL-1 )genotype and/or alleles were associated with aggressive periodontitis (AgP) patients and their relatives. METHODS: Sixty-six AgP nuclear families including 66 probands and their 114 relatives were recruited. All probands were diagnosed as AgP according to 1999 classification of periodontal diseases and conditions, the diagnoses of their relatives were using a system of assessment (including the current and retrospective diagnosis and reported case history). Anti-coagulated peripheral blood samples were collected from all of the 180 subjects, and DNA was extracted from each blood sample. Single nucleotide polymorphisms (SNPs) at IL-1A +4845 and IL-1B j 511 were analyzed by PCR-RFLP assay. The polymorphism of a variable number of tandem repeat (VNTR) in intron 2 of IL-1RN was detected by PCR amplification and fragment size analysis. The distributions of genotypes and/or alleles in all groups were analyzed by logistic regression analysis. Data were also analyzed using the family-based association test. RESULTS: The frequency of all markers (at IL-1A +4845 and IL-1B -511 and VNTR) showed no association wih AgP in relatives (P>0.05). Tests of association by Family-Based Association Test showed no difference in the distribution of the allele 1 of IL-1B-511 under all three genetic models tested (Additive: Z=0.389, P=0.6969; Dominant: Z=0.089, P=0.9287; Recessive: Z=0.089, P=0.9287). The distribution of the allele 1 of IL- 1A+4845 also showed no significant difference under all three models (P>0.05). But when all AgP patients were stratified by the clinical subtype, the frequencies of A1A1 genotype and allele 1 at IL-1B-511 were significantly increased in the localized subtype AgP patients compared to unaffected (genotype: 43.8% vs 22.8%,OR=7.32,95% CI=1.84-29.11,P=0.005; allele 1: 71.9% vs 41.2%,OR=3.64,95% CI=1.55-8.58,P=0.002), while no significant difference was observed between the generalized subtype and the unaffected (genotype: P=0.88, allele: P=0.64). CONCLUSION: The polymorphism of IL-1B-511 may have an effect on the susceptibility of localized AgP in Chinese population. It should be tested in larger family samples.


Assuntos
Periodontite Agressiva/genética , Interleucina-1/genética , Adulto , Alelos , Feminino , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Linhagem , Polimorfismo de Nucleotídeo Único , Estudos Retrospectivos , Adulto Jovem
9.
Zhonghua Gan Zang Bing Za Zhi ; 14(4): 258-60, 2006 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-16635292

RESUMO

OBJECTIVES: To explore the diagnostic value of color Doppler flow imaging (CDFI) in monitoring portal vein complications (PVC) following orthotopic liver transplantation (OLT). METHODS: Five hundred and four patients received OLT and had CDFI examinations before and after their transplantations. CDFI monitoring parameters before the operation included portal vein diameter, blood flow velocity and the presence of thrombi within the portal vein. Monitoring parameters after the operation included portal vein diameters of donor and receiver sides, and the diameter of the mouth of anastomosis, inner side of blood vessel echo, the direction of blood flow and its speed. RESULTS: Of the 504 patients examined, the median velocity of the portal blood flow was 46.27 cm/s (range: 15.8 to 110.8) on the day of the operation. The blood flow speed of 358 cases (71.03%) was higher than 40 cm/s. Of the 358 patients, 347 (68.85%) had a blood flow speed lower than 40 cm/s one month after the operation. Sixty-four patients (12.70%) showed hepatofugal blood flow once, and only one case had a total hepatofugal blood flow. Thirteen patients (2.58%) had portal vein complications, including 4 cases with portal vein thrombosis and 9 with portal vein stenosis. CONCLUSION: Not all abnormal portal vein blood flow signals will lead to complications. It is worthwhile to monitor the portal blood flows. CDFI plays an important role in the diagnosis of portal vein complications after orthotopic liver transplantation.


Assuntos
Neoplasias Hepáticas/cirurgia , Transplante de Fígado/efeitos adversos , Veia Porta/diagnóstico por imagem , Ultrassonografia Doppler Dupla , Trombose Venosa/diagnóstico por imagem , Adolescente , Adulto , Idoso , Velocidade do Fluxo Sanguíneo/fisiologia , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Cirrose Hepática/cirurgia , Transplante de Fígado/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Ultrassonografia Doppler Dupla/métodos , Ultrassonografia de Intervenção , Trombose Venosa/etiologia
10.
J Opt Soc Am A Opt Image Sci Vis ; 22(2): 385-90, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15717571

RESUMO

We analyze a method for efficiently generating optical vortices by use of annular computer-generated holograms and a spatial light modulator. We found that there exists an optimal annular width by which the reconstructed vortex ring in the focal plane has the steepest gradient and the worthless subbright rings can be largely suppressed. We fitted a general formula for determining the value of this optimal annular width and propose a method for designing a multiring structure of optical vortices and specialized interferometric vortex patterns. Finally, we discuss the situation of a Gaussian beam as illuminated light and find that there exists an optimal beam waist that results in the best energy efficiency.

11.
Shanghai Kou Qiang Yi Xue ; 13(5): 365-6, 2004 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-15514856

RESUMO

PURPOSE: The goal of this study was to explore the relationship between feeding methods and deciduous caries. METHODS: An investigation on children's feeding methods, caries prevalence and dmft was made, using 260 cases which were provided with whole primary teeth. Chi-square test was used for statistical analysis on feeding methods and caries prevalence, and variance analysis was used on feeding methods and dmft. RESULTS: The results showed that the deciduous caries rate, mean dmft in breast feeding (BF) group were lower than those in mixed feeding (MF) group and artificial feeding (AF) group, there were significant differences between BF group and MF group,also between BF group and AF group. But there were no significant differences between MF group and AF group. CONCLUSION: It is concluded that proper breast feeding seems to be important for preventing deciduous caries.


Assuntos
Cárie Dentária/epidemiologia , Métodos de Alimentação , Dente Decíduo , Fatores Etários , Aleitamento Materno/estatística & dados numéricos , Criança , Humanos , Prevalência
12.
Opt Express ; 12(21): 5166-71, 2004 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-19484073

RESUMO

We report a new optical vortex phase-shifting method for digital holography, in which an optical vortex mode is taken as the reference beam for holographic recording, and the required phase shifts are directly generated by rotating the vortex mode. In digital reconstruction, the complex amplitude of the object wave can be retrieved by use of the conventional phase shifting algorithm on condition that the digital illumination beam is replaced by an vortex beam with the same topological charge as the reference used. Both the theoretical analysis and experimental results demonstrate the feasibility of this approach.

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