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1.
Chem Senses ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32010937

RESUMO

Neuropeptide S (NPS) is an endogenous peptide recently recognized to be presented in the brainstem, and believed to play an important role in maintaining memory. The deletion of NPS or NPS receptor (NPSR) in mice shows a deficit in memory formation. Our recent studies have demonstrated that central administration of NPS facilitates olfactory function and ameliorates olfactory spatial memory impairment induced by muscarinic cholinergic receptor antagonist and N-methyl-D-aspartate receptor antagonist. However, it remains to be determined if endogenous NPS is an indispensable neuromodulator in the control of the olfactory spatial memory. In this study, we examined the effects of NPSR peptidergic antagonist [D-Val5]NPS (10 and 20 nmol, i.c.v.) and nonpeptidergic antagonist SHA 68 (10 and 50 mg/kg, i.p.) on the olfactory spatial memory using computer-assisted 4-hole-board olfactory spatial memory test in mice. Furthermore, immunofluorescence was employed to identify the distributions of c-Fos and NPSR immunoreactive (-ir) neurons in olfactory system and hippocampal formation known to closely relate to the olfactory spatial memory. [D-Val5]NPS dosing at 20 nmol and SHA 68 dosing at 50 mg/kg significantly decreased the number of visits to the two odorants interchanged spatially, switched odorants, in recall trial, and simultaneously reduced the percentage of Fos-ir in NPSR-ir neurons which were densely distributed in the anterior olfactory nucleus (AON), piriform cortex (Pir), subiculum (S), presubiculum (PrS) and parasubiculum (PaS). These findings suggest that endogenous NPS is a key neuromodulator in olfactory spatial memory.

2.
Math Biosci Eng ; 16(5): 4559-4580, 2019 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-31499677

RESUMO

In this paper, we propose a robust video steganographic method, which can efficiently hide confidential messages in video sequences, and ensure that these messages are perfectly reconstructed by recipient. To apply proposed scheme to video sequences, we must be faced with two nontrivial problems: (a) how to effectively minimize the total steganographic distortion for each video frame? (b) how to recover the hidden messages if some frames are lost or damaged? We tackle the first question by designing a new distortion function, which employs two continuous adjacent frames with the same scene as side-information. The second question is addressed by data sharing. In this mechanism, the original data is expanded and split into multiple shares by using multi-ary Vandermonde matrix. Since these shares contain a lot of data redundancy, the recipient can recover the hidden data even if some frames are damaged or lost during delivery. Extensive experiments show that proposed scheme outperforms the state-of-the-arts in terms of robustness and diverse attacks.

3.
J Therm Biol ; 83: 134-141, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31331511

RESUMO

The rainbow trout is a cold-water fish cultured in China. Heat stress has a serious impact on the summer survival and the yield of rainbow trout. A better understanding of the regulatory response of rainbow trout to heat stress will help in determining the relationship between heat stress signaling pathways and adaption mechanisms and help contribute to breeding new high-temperature tolerant strains of rainbow trout. In this study, the 48-h median lethal temperature (48h-LT50) of rainbow trout was determined as 22.5°C. We developed control (16°C) and heat-treated (22.5°C) groups and extracted RNA from the head kidney tissues for high-throughput sequencing to study the microRNA (miRNA) expression profiles. Twelve up-regulated and five down-regulated miRNAs were identified between the control and heat-treated groups. A total of 22 target genes were predicted for 6 of the differentially expressed miRNAs, including 31 negative miRNA-mRNA interactions. Important regulatory pathways under heat stress are related to the metabolism and immune responses of the rainbow trout. Our findings provide preliminary data for investigating the high-temperature molecular mechanism of the rainbow trout and can help producers to reduce the economic losses caused by high temperature weather.


Assuntos
Resposta ao Choque Térmico , Rim/metabolismo , MicroRNAs/genética , RNA Mensageiro/genética , Truta/metabolismo , Animais , MicroRNAs/metabolismo , RNA Mensageiro/metabolismo , Truta/genética
4.
J Zhejiang Univ Sci B ; 20(1): 23-31, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30614227

RESUMO

With the rapid development of immunology, molecular biology, and associated technologies such as next-generation sequencing, cellular immunotherapy has recently become the fourth major cancer treatment. Immunotherapies based on T cells, natural killer cells, and dendritic cells play key roles in cancer immunotherapy. However, their application in clinical practice raises several ethical issues. Thus, studies should focus on proper adherence to basic ethical principles that can effectively guide and solve related clinical problems in the course of treatment, improve treatment effects, and protect the rights and interests of patients. In this review, we discuss cellular immunotherapy-related ethical issues and highlight the ethical practices and current status of cellular immunotherapy in China. These considerations may supplement existing ethical standards in cancer immunotherapy.


Assuntos
Imunoterapia/ética , Neoplasias/terapia , China , Células Dendríticas/imunologia , Humanos , Imunidade Celular , Imunoterapia/métodos , Células Matadoras Naturais/imunologia , Neoplasias/imunologia , Seleção de Pacientes/ética , Linfócitos T/imunologia
5.
Cell Physiol Biochem ; 41(1): 91-100, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28214858

RESUMO

BACKGROUND: Hepatitis B virus (HBV) causes both acute and chronic liver injury. Viral proteins are involved in the pathological progress. Hepatitis B core antigen (HBcAg), a component of viral nucleocapsid, is not only essential for HBV lifecycle, but also exhibits strong immunogenicity. The cytoplasmic location of HBcAg in liver biopsy is associated with liver injury and inflammation, but the exact mechanisms remain to be elaborated. METHODS: Huh7, SMMC-7721 and L-02 cells were transfected with pEGFP-N1-HBcAg to establish an intracellular HBcAg expression model. The mRNA and protein levels of Interleukin (IL)-6 were detected by qPCR and ELISA respectively. The signaling pathway-related proteins were investigated by western blot and immunofluorescence assay. RESULTS: HBcAg increased the expression and secretion of IL-6 through activating extracellular signal-related kinase (ERK), p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor-kappa B (NF-κB). These activations can be blocked by specific inhibitors of the three pathways. CONCLUSIONS: HBcAg actives p38, ERK1/2 and NF-κB to enhance the production of IL-6 in hepatocytes. This provides a molecular mechanism to explain the association of cytoplasmic HBcAg with severe liver injury and inflammation.


Assuntos
Antígenos do Núcleo do Vírus da Hepatite B/metabolismo , Interleucina-6/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , NF-kappa B/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Linhagem Celular , Ensaio de Imunoadsorção Enzimática , Antígenos do Núcleo do Vírus da Hepatite B/genética , Vírus da Hepatite B/metabolismo , Hepatócitos/citologia , Hepatócitos/metabolismo , Humanos , Interleucina-6/análise , Interleucina-6/genética , Microscopia de Fluorescência , RNA Mensageiro/metabolismo , Transdução de Sinais
6.
PLoS One ; 11(7): e0159089, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27434097

RESUMO

During hepatitis B virus (HBV) infection, three viral envelope proteins of HBV are overexpressed in the endoplasmic reticulum (ER). The large S protein (LHBs) and truncated middle S protein (MHBst) have been documented to play roles in regulating host gene expression and contribute to hepatic disease development. As a predominant protein at the ultrastructural level in biopsy samples taken from viremic patients, the role of the middle S protein (MHBs) remains to be understood despite its high immunogenicity. When we transfected hepatocytes with an enhanced green fluorescent protein (EGFP)-tagged MHBs expressing plasmid, the results showed that expression of MHBs cause an upregulation of IL-6 at the message RNA and protein levels through activating the p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor-kappa B (NF-κB) pathways. The use of specific inhibitors of the signaling pathways can diminish this upregulation. The use of BAPTA-AM attenuated the stimulation caused by MHBs. We further found that MHBs accumulated in the endoplasmic reticulum and increased the amount of glucose regulated protein 78 (GRP78/BiP). Our results provide a possibility that MHBs could be involved in liver disease progression.


Assuntos
Vírus da Hepatite B/genética , Hepatite B/genética , Hepatite B/virologia , Interações Hospedeiro-Patógeno/genética , Proteínas do Envelope Viral/genética , Linhagem Celular , Retículo Endoplasmático/genética , Retículo Endoplasmático/virologia , Estresse do Retículo Endoplasmático/genética , Regulação Viral da Expressão Gênica , Proteínas de Fluorescência Verde/genética , Hepatite B/patologia , Vírus da Hepatite B/patogenicidade , Humanos , Interleucina-6/biossíntese , NF-kappa B/genética , Fosforilação/genética , Transdução de Sinais , Fator de Transcrição RelA/genética , Proteínas do Envelope Viral/biossíntese , Proteínas Quinases p38 Ativadas por Mitógeno/genética
7.
Chem Biodivers ; 13(5): 596-601, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27088891

RESUMO

Following an in vitro bioactivity-guided fractionation procedure, 14 compounds including eight flavonoids and six phenylpropanoids were isolated and identified from the AcOEt fraction of Clinopodium chinense (Benth.) O. Kuntze. All constituents were tested for α-glucosidase and high glucose-induced injury in human umbilical vein endothelial cells (HUVECs) inhibitory activities. All constituents exhibited varying degrees α-glucosidase inhibitory activity and protective activity on HUVECs. Among them, luteolin (2), eriodictyol (5), ethyl rosmarinate (13), and clinopodic acids B (14) were proved to be potent α-glucosidase inhibitors with IC50 value ranging from 0.6 to 2.0 µm. Additionally, luteolin (2), naringenin (4), eriodictyol (5), ethyl (2R)-3-(3, 4-dihydroxyphenyl)-2-hydroxypropanate (9), caffeic acid (11), ethyl rosmarinate (13), and clinopodic acids B (14) significantly ameliorate HUVECs injury induced by high glucose with an approximate EC50 value of 3 - 36 µm. These results suggest that the 14 bioactive constituents were responsible for hypoglycemic and protective vascular endothelium effect of C. chinense (Benth.) O. Kuntze and their structure-activity relationship was also analyzed briefly. Eriodictyol, luteolin, ethyl rosmarinate, and clinopodic acids B were the potential lead compounds of antidiabetic drugs.


Assuntos
Inibidores de Glicosídeo Hidrolases/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Lamiaceae/química , Fenóis/farmacologia , alfa-Glucosidases/metabolismo , Relação Dose-Resposta a Droga , Glucose/farmacologia , Inibidores de Glicosídeo Hidrolases/química , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Células Endoteliais da Veia Umbilical Humana/patologia , Humanos , Estrutura Molecular , Fenóis/química , Fenóis/isolamento & purificação , Relação Estrutura-Atividade
8.
Zhongguo Dang Dai Er Ke Za Zhi ; 17(5): 440-4, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26014691

RESUMO

OBJECTIVE: To study the clinical and imaging features of premature infants with different degrees of bronchopulmonary dysplasia (BPD). METHODS: A prospective study was performed on the clinical data of 59 premature infants (gestational age <32 weeks) with BPD. Among the 59 premature infants, 37 cases had mild BPD and the other 22 cases had moderate to severe BPD. The clinical and imaging data were compared between these premature infants with different degrees of BPD. RESULTS: The durations of mechanical ventilation, oxygen therapy, antibiotic therapy, parenteral nutrition, and hospitalization in the moderate to severe group were significantly longer than those in the mild group (P<0.05). The incidence of nosocomial infection and number of times of red blood cell transfusion in the moderate to severe group were significantly higher than that in the mild group. The rates of X-ray changes, including grade I respiratory distress syndrome (1 day after birth) and hypolucency of lungs (4-10 days and ≥ 28 days after birth) were significantly higher in the mild group than in the moderate to severe group. The rates of X-ray changes in classical BPD stage III (4-10 days after birth) and IV (≥ 28 days after birth) were significantly higher in the moderate to severe group than in the mild group. CONCLUSIONS: The durations of mechanical ventilation, oxygen therapy, and antibiotic therapy and the incidence of nosocomial infection are correlated with the severity of BPD. The premature infants with severer BPD need a longer duration of parenteral nutrition and more times of red blood cell transfusion and have more typical imaging changes of BPD. Imaging examination has a predictive value for the severity of BPD.


Assuntos
Displasia Broncopulmonar/diagnóstico por imagem , Feminino , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Masculino , Radiografia Torácica , Tomografia Computadorizada por Raios X
9.
Sci Rep ; 5: 9231, 2015 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-25782417

RESUMO

Quantum digital signatures can be used to authenticate classical messages in an information-theoretically secure way. Previously, a novel quantum digital signature for classical messages has been proposed and gave an experimental demonstration of distributing quantum digital signatures from one sender to two receivers. Some improvement versions were subsequently presented, which made it more feasible with present technology. These proposals for quantum digital signatures are basic building blocks which only deal with the problem of sending single bit messages while no-forging and non-repudiation are guaranteed. For a multi-bit message, it is only mentioned that the basic building blocks must be iterated, but the iteration of the basic building block still does not suffice to define the entire protocol. In this paper, we show that it is necessary to define the entire protocol because some attacks will arise if these building blocks are used in a naive way of iteration. Therefore, we give a way of defining an entire protocol to deal with the problem of sending multi-bit messages based on the basic building blocks and analyse its security.

10.
Bing Du Xue Bao ; 24(4): 249-54, 2008 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-18780625

RESUMO

The cross reactivity of mimotopes of hepatitis C virus (HCV) hypervariable region 1 (HVR1) was investigated to obtain epitopes that have high cross reactivity. Five expression vectors encoding B cell mimotopes fused with Trx were constructed, and the mimotope proteins were purified. The cross reactivity of mimotope proteins with HCV positive sera was determined by ELISA. HCV pseudotype particles (HCVpp) were generated and applied to evaluate neutralization effects of the sera of BALB/c mice immuned with the mimotope proteins on infection of Huh7. 5 cells. Our data showed that the mimotope proteins (P1, P2, P5, P6, P8) could react to the HCV positive sera. The HCVpp infection inhibition of the sera of BALB/c mice immuned with P6 or P8 was detectable. These results suggest that the mimotopes may be valuable in the studies of anti-HCV infection and development of HCV vaccines.


Assuntos
Regiões Determinantes de Complementaridade/imunologia , Hepacivirus/imunologia , Sequência de Aminoácidos , Animais , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , Epitopos , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular
11.
Org Lett ; 10(10): 1905-8, 2008 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-18429615

RESUMO

Cipadonoid A ( 1), a novel limonoid with an unprecedented skeleton, was isolated from the leaves of Cipadessa cinerasecns. Its structure and relative configuration were determined by spectroscopic analysis and computer modeling. 1 represents a new type of limonoid, characterized by a rearranged tetrahydropyranyl ring B incorporating usually exocyclic C-30. A possible biosynthetic pathway of 1 was also proposed.


Assuntos
Medicamentos de Ervas Chinesas/química , Limoninas/química , Meliaceae/química , Plantas Medicinais/química , Dicroísmo Circular/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Limoninas/isolamento & purificação , Espectroscopia de Ressonância Magnética/métodos , Espectroscopia de Ressonância Magnética/normas , Modelos Químicos , Folhas de Planta/química , Padrões de Referência , Espectrofotometria Ultravioleta/métodos
12.
J Nat Prod ; 70(8): 1352-5, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17655260

RESUMO

Four new trijugin-type limonoids, cipatrijugins A-D (1-4), together with the known cipadesin A (5), were isolated from the leaves of Cipadessa cinerascens, and their structures were elucidated on the basis of spectroscopic and computational methods. The ability of compounds 1-5 to inhibit the growth of the A549 and K562 tumor cell lines was evaluated.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Limoninas/isolamento & purificação , Meliaceae/química , Plantas Medicinais/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Limoninas/química , Limoninas/farmacologia , Estrutura Molecular , Folhas de Planta/química
13.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 26(3): 298-301, 2004 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-15266834

RESUMO

OBJECTIVE: To explore the role of eotaxin in the pathogenesis of bronchial asthma and the clinical value in the diagnosis of asthma. METHODS: Serum eotaxin were measured by ELISA in 38 patients with asthma, 28 patients with non-asthma allergy, and 30 healthy controls. RESULTS: The levels of serum eotaxin in the asthma group were higher than those in the non-asthma allergic and control group (P<0.01). Furthermore, eotaxin levels in patients with acute asthma were significantly higher than those in patients with stable asthma (P<0.001). It was also found that the eotaxin levels of the acute asthma group were positively correlated to the amounts of eosinophils in peripheral blood (r=0.4196, P<0.001), and inversely correlated to the forced expiratory volume in one second (FEV1) (r=-0.3746, P<0.001). CONCLUSION: It suggests that eotaxin may play a crucial pathogenic role in the asthmatic process possibly by activating the allergic inflammatory cells and controlling the recruitment of eosinophils from blood to bronchial epithelium of the airway. The concentration of eotaxin is significantly associated with the attack of acute asthma and its severity. Eotaxin may be a potential therapeutic target in patients with asthma.


Assuntos
Asma/diagnóstico , Quimiocinas CC/sangue , Adulto , Asma/fisiopatologia , Contagem de Células , Quimiocina CCL11 , Quimiocinas CC/fisiologia , Eosinofilia/patologia , Feminino , Volume Expiratório Forçado , Humanos , Masculino , Pessoa de Meia-Idade
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