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1.
Anim Biotechnol ; : 1-7, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31424334

RESUMO

Detection of reactive oxygen species (ROS) is of great interest in semen analysis since their excess is detrimental to sperm function and male fertility. Fluorescence microscopy has achieved attention for providing broad possibilities of sperm evaluations and also for presenting substantial accessibility. In this context, this study investigated the efficiency of CellROX Deep Red® and Orange® probes in detecting ROS in bovine sperm cells and assessed their relationship with sperm fertility potential. First, 16 ejaculates were assigned in three treatments: T0 (no ROS production induced), T1x (ROS production induced once) and T2x (ROS production induced twice). Samples were incubated with Red and Orange probes and percentages of cells producing ROS were evaluated using fluorescence microscopy. Coefficient of determination was 0.61 for Red and 0.56 for Orange. Afterwards, frozen-thawed semen samples from high and low fertility bulls were evaluated regarding percentages of cells producing ROS detected by Red and Orange. Higher levels of ROS assessed by Red were detected in low fertility bovine samples. In conclusion, CellROX Red® and Orange® are both efficient in detecting ROS in bovine spermatozoa. Furthermore, higher sperm ROS detection by CellROX Red® might be associated with low fertility samples.

2.
Braz Dent J ; 30(1): 36-42, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30864645

RESUMO

Maltodextrins, derived from corn starch, have been added to industrialized food combined with sucrose. However it is not clear the diffusion properties of the dental biofilm matrix and the tridimensional structure of multispecies biofilms formed in the presence of these carbohydrates. Therefore, the aim of study was to investigate by confocal laser scanning microscopy (CLSM) the structural organization of the multispecies dental biofilm formed in situ under exposure to sucrose associated to maltodextrin. Adult volunteers wore an intraoral palatal appliance containing bovine enamel blocks. They were instructed to remove the appliance 8 times per day and drop the following solutions on the enamel blocks: deionized distilled water (DDW), maltodextrin, sucrose + maltodextrin or sucrose. Biofilms formed were stained and the percentage of extracellular polysaccharide (%EPS) and thickness were determined by CLSM. Biofilm formed in the presence of sucrose and sucrose + maltodextrin presented similar %EPS and higher than DDW and maltodextrin. Regarding to the biofilm thickness, sucrose and sucrose + maltodextrin treatments were thicker than DDW and maltodextrin and similar between them. The structural organization of the multispecies dental biofilm formed in situ in the presence of sucrose does not change when this carbohydrate is associated to maltodextrin.


Assuntos
Biofilmes , Polissacarídeos/metabolismo , Sacarose/metabolismo , Adulto , Estudos Cross-Over , Método Duplo-Cego , Humanos , Microscopia Confocal , Aparelhos Ortodônticos , Adulto Jovem
3.
Int J Paediatr Dent ; 29(4): 456-463, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30807677

RESUMO

BACKGROUND: Asthma is a chronic disease that involves several healthcare services that need attention in relation to its treatment and prevention. AIM: The objective of this study was to evaluate caries, erosion, and enamel defects in children with and without asthma. DESIGN: Cross-sectional study, with a sample of 228 children ages 6-12 years, stratified as asthmatic (n = 112) and non-asthmatic (n = 116), at two Primary Health Units of the Community Health Service, Brazil. The evaluation consisted of an oral examination and a structured interview with parents/guardians, in addition to data from medical records. Data were analysed by Poisson regression with a robust error variance, all of them at a level of significance of P < 0.05. RESULTS: Of 112 asthmatic children, 63 (51.2%) had dental caries and 25 (53.2%) enamel defects. In the adjusted analysis, dental caries and use of salbutamol were associated (PR = 1.32, 95% CI = 1.01-1.72). In addition, children who performed oral hygiene more than three times a day showed a greater prevalence (PR = 2.36, 95% CI = 1.02-4.85) of dental erosion compared with children who performed it only once a day. CONCLUSIONS: There is no evidence for an association between asthma, caries, erosion, and enamel defect in children aged from 6 to 12 years. There was, however, an association between dental caries and use of salbutamol.

4.
Braz. dent. j ; 30(1): 36-42, Jan.-Feb. 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-989435

RESUMO

Abstract Maltodextrins, derived from corn starch, have been added to industrialized food combined with sucrose. However it is not clear the diffusion properties of the dental biofilm matrix and the tridimensional structure of multispecies biofilms formed in the presence of these carbohydrates. Therefore, the aim of study was to investigate by confocal laser scanning microscopy (CLSM) the structural organization of the multispecies dental biofilm formed in situ under exposure to sucrose associated to maltodextrin. Adult volunteers wore an intraoral palatal appliance containing bovine enamel blocks. They were instructed to remove the appliance 8 times per day and drop the following solutions on the enamel blocks: deionized distilled water (DDW), maltodextrin, sucrose + maltodextrin or sucrose. Biofilms formed were stained and the percentage of extracellular polysaccharide (%EPS) and thickness were determined by CLSM. Biofilm formed in the presence of sucrose and sucrose + maltodextrin presented similar %EPS and higher than DDW and maltodextrin. Regarding to the biofilm thickness, sucrose and sucrose + maltodextrin treatments were thicker than DDW and maltodextrin and similar between them. The structural organization of the multispecies dental biofilm formed in situ in the presence of sucrose does not change when this carbohydrate is associated to maltodextrin.


Resumo Maltodextrinas, derivadas do amido de milho, tem sido adicionadas a alimentos industrializados combinadas à sacarose. Entretanto não estão esclarecidas as propriedades de difusão na matriz do biofilme dental e a estrutura tridimensional de biofilmes multiespécies formados na presença destes carboidratos. Portanto o objetivo deste estudo foi avaliar, através da microscopia confocal de escaneamento a laser (MCEL), a organização estrutural do biofilme dentário multiespécie formado in situ exposto à sacarose associada a maltodextrina. Voluntários adultos utilizaram dispositivos intraorais palatinos contendo blocos de esmalte bovino. Eles foram instruídos a remover os dispositivos 8 vezes por dia e gotejar as seguintes soluções sobre os blocos de esmalte: água destilada e deionizada (ADD), maltodextrina, sacarose+maltodextrina ou sacarose. Os biofilmes formados foram corados e o percentual de polissacarídeos extracelulares (%PEC) e suas espessuras foram determinados através da MCEL. Os biofilmes formados na presença de sacarose e sacarose+maltodextrina apresentaram os %PEC similares entre si, entretanto maiores do que os grupos submetidos a ADD e maltodextrina. Em relação à espessura do biofilme formado, os tratamentos sacarose e sacarose+maltodextrina apresentaram espessuras similares entre si, e maiores quando comparados aos grupos ADD e maltodextrina. A organização estrutural do biofilme dentário multiespécie formado in situ na presença de sacarose não é alterado quando este carbiodrato é associado a maltodextrina.

5.
Cad. Bras. Ter. Ocup ; 27(1): 61-71, Jan.-Mar. 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-989494

RESUMO

Abstract: Introduction: Communication, social interactions and health-related quality of life (HRQoL) of the person affected by head and neck cancer (HNC) are affected by both diagnosis and treatment. Objective: The aim of this study was to evaluate the use of alternative communication software as a resource for the application of HRQoL assessment tools for people with HNC who underwent laryngectomy. Method: An exploratory, cross-sectional study with 100 adult individuals of both genders. A Sociodemographic identification form was used and the Brazilian Criteria for Economic Classification was applied the Functional Assessment of Cancer Therapy-Head and Neck-FACT-H&N scale. These instruments were inserted, in their original format, into the alternative communication software Livox®, which is an auxiliary communication resource that favors the communication of people with speech difficulties providing a conversion of text into sounds. Results: The dimensions of functional well-being and emotional well-being were the most affected in the evaluation of HRQoL. Most interviewees did not have difficulty in using Livox® to respond to the questionnaires. However, there was an association between the age and professional activity in relation to the use of the software, since 100% of the over-58s and non-active retirees reported some difficulties in their use. Conclusion: The use of technological resources may facilitate access to services and treatments by laryngectomized individuals, however, the elderly present greater difficulties in the use of modern communication technologies due to sociocultural contexts, cognitive and emotional difficulties. Occupational therapy can facilitate this adaptation through the use of resources, strategies, and techniques for the use of technology instruments as facilitators for communication in intra- and extra-hospital contexts, providing autonomy and independence for the subjects.


Resumo Introdução: A comunicação, as interações sociais e a qualidade de vida relacionada à saúde (QVRS) da pessoa acometida pelo câncer de cabeça e pescoço (CCP), são afetadas tanto pelo diagnóstico como pelos tratamentos. Objetivo: Avaliar o uso de um software de comunicação alternativa como recurso para aplicação de instrumentos de avaliação da QVRS de pessoas com CCP, submetidas à laringectomia. Método: Estudo exploratório, transversal, realizado com 100 pessoas adultas, de ambos os sexos. Foi utilizada uma ficha de identificação Sociodemográfica e foram aplicados o Critério de Classificação Econômica Brasil e a escala Functional Assessment of Cancer Therapy - Head and Neck - FACT - H & N. Esses instrumentos foram inseridos, em seu formato original, no software de comunicação alternativa Livox®, que é um recurso auxiliar de comunicação que favorece a comunicação de pessoas com dificuldades na fala, fornecendo uma conversão de texto em sons. Resultados: As dimensões do Bem-Estar Funcional e Bem-Estar Emocional foram as mais comprometidas na avaliação da QVRS. Os entrevistados, em sua maioria, não demonstraram dificuldade em utilizar o Livox® para responder aos questionários. Porém, observou-se associação entre a idade e atividade profissional exercida em relação à utilização do software, pois 100% das pessoas com mais de 58 anos e os aposentados não ativos referiram alguma dificuldade em sua utilização. Conclusão: O uso de recursos tecnológicos pode facilitar acessos à serviços e tratamentos por parte das pessoas laringectomizadas, mas os idosos apresentam maiores dificuldades no uso de tecnologias modernas de comunicação, devido à contextos socioculturais, dificuldades cognitivas e emocionais. A terapia ocupacional pode facilitar essa adaptação através do uso de recursos, estratégias e técnicas de uso de instrumentos de tecnologia como facilitadores para comunicação em contextos intra e extra-hospitalares, proporcionando autonomia e independência aos sujeitos.

6.
RGO (Porto Alegre) ; 66(3): 257-262, July-Sept. 2018.
Artigo em Inglês | LILACS-Express | ID: biblio-984910

RESUMO

ABSTRACT Carbohydrates are largely present in our diet. Sucrose the most commonly consumed carbohydrat and presents a high cariogenic potential. Starch has low cariogenic potential but this effect may be increased if it is consumed in combination with a sucrose-rich diet due to a prolonged retention on tooth surfaces. Maltodextrin is derived from the acid hydrolysis and/or enzymatic hydrolysis of corn starch and it is increasingly present in a variety of industrialized foods such as infant formulas, sports drinks and energy supplements. Yet, its role in the development of dental caries is not clear. The objective of this study was to conduct a literature review of the association between maltodextrin and dental caries. Based on the studies included in this review it can be concluded that maltodextrin has an acidogenic potential lower than sucrose, and that there is a lack of studies about the association between maltodextrin and sucrose and it may not be possible to assess the relationship to dental caries.


RESUMO Os carboidratos são amplamente presentes em nossa dieta. A sacarose é o carboidrato mais comumente consumido e apresenta um alto potencial cariogênico. O amido apresenta um baixo potencial cariogênico, mas este efeito pode ser aumentado se for consumido em combinação com uma dieta rica em sacarose, devido a uma retenção prolongada nas superfícies dos dentes. A maltodextrina é derivada a partir da hidrólise ácida e/ou enzimática do amido de milho e está cada vez mais presente em uma variedade de alimentos industrializados, como fórmulas infantis, bebidas esportivas e suplementos energéticos. Contudo, o seu papel no desenvolvimento da cárie dentária ainda não está esclarecido. O objetivo deste estudo foi realizar uma revisão da literatura sobre a associação entre a maltodextrina e a cárie dentária. Com base nos estudos incluídos nesta revisão, pode-se concluir que a maltodextrina apresenta um potencial acidogênico menor do que a sacarose, e que estudos sobre a associação entre maltodextrina e sacarose são escassos não podendo ser possível avaliar sua relação com cárie dentária.

7.
Rev. bras. oftalmol ; 77(4): 175-179, jul.-ago. 2018. tab, graf
Artigo em Português | LILACS-Express | ID: biblio-959099

RESUMO

Resumo Objetivo: Avaliar o perfil epidemiologico de disfuncao da acuidade visual (AV), possiveis disturbios oculares e a frequencia ao atendimento oftalmologico, de escolares do municipio de Itauna, Minas Gerais, Brasil. Posteriormente objetivou-se o encaminhamento ao especialista para correcao das anormalidades. Métodos: Estudo transversal, com populacao avaliada de 432 alunos da rede publica de ensino. Os individuos foram avaliados pelo metodo Snellen e a faixa etaria da amostra variou de 4 a 17 anos. Os dados foram colhidos e sistematizados. Foram encaminhados para o servico de Oftalmologia aqueles que possuiam AV ≤ 0,7 em pelo menos um dos olhos. Resultados: Dos 432 alunos avaliados neste estudo 14,5% apresentaram baixa AV, quando avaliados pelo teste de Snellen. Destes, 61,9 % pertenciam ao sexo feminino. A faixa etaria em que houve maior prevalencia de baixa visao foram escolares de 15 a 17 anos. A maioria apresentou alteracao em ambos os olhos e 60% dos alunos avaliados declararam nunca terem passado por uma consulta oftalmologica. Dos alunos que compareceram as consultas, a maioria foi diagnosticada com erros de refracao e necessitou de correcao otica. Conclusão: Os resultados encontrados demonstram que a parcela significativa dos individuos avaliados apresentou baixa visao e necessitou de encaminhamento oftalmologico. Alem disso, observou-se a inexistencia de consultas oftalmológicas anteriores em grande parte dos escolares. Esse fato reforca, diante dos orgaos publicos de saude, a necessidade de implantar sistemas de triagem visual nas escolas e oferecer assistencia a esses estudantes, objetivando melhorias em seu aprendizado e qualidade de vida.


ABSTRACT Objective: To evaluate the epidemiological profile of visual acuity (VA) dysfunction, the possible ocular disorders and the frequency of ophthalmologic care of school children from the city of Itaúna, Minas Gerais, Brazil. Methods: A cross-sectional study with a population of 432 students in the public schools. The individuals were evaluated by the Snellen method and the sample age ranged from 4 to 17 years. Data were collected and systematized. Those who had VA ≤ 0.7 in at least one eye were referred to the ophthalmology service. Results: Of the 432 students evaluated in this study, 14.5% presented low VA, when evaluated by the Snellen test. Of these, 61.9% were female. The age group with the highest prevalence of low vision were schoolchildren aged 15 to 17 years. The majority presented alteration in both eyes and 60% of the evaluated students stated that they had never had an ophthalmological consultation. Of the students who attended the consultations, most were diagnosed with refractive errors and needed optical correction. Conclusion: The results showed that a significant portion of the individuals evaluated had low vision and required ophthalmologic referral. In addition, there was no previous ophthalmological consultation in most of the schoolchildren. This fact reinforces to public health agencies, the need to implement visual screening systems in schools and offer assistance to these students, with the aim of improvements in their learning and quality of life

8.
J Glob Infect Dis ; 10(2): 42-46, 2018 Apr-Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29910563

RESUMO

Introduction: Sepsis is a systemic inflammatory response to suspected or confirmed infection. Clinical evaluations are essential for its early detection and treatment. Blood cultures may take as long as 2 days to yield a result and are not always reliable. However, recent studies have suggested that neutrophil CD64 expression may be a sensitive and specific alternative for the diagnosis of systemic infection. Objective: The objective of the study was to analyze the difference in CD64 values between subjects with systemic inflammatory response syndrome (SIRS), suspected or confirmed sepsis, who meet diagnostic criteria for SIRS upon arriving at an emergency department. Materials and Methods: This was a prospective observational cohort study, an accuracy study of CD64 prospectively evaluated. The sample consisted of 109 patients aged 18 years with criteria for SIRS on arrival to emergency department. CD64 expression was measured within 6 h of hospital admission and once again after 48 h. Results: ROC curve analysis suggested that a cutoff of 1.45 for CD64 expression could diagnose sepsis with a sensitivity of 0.85, a specificity of 0.75, an accuracy of 82.08%, a positive predictive value of 0.96, a negative predictive value of 0.38 and a positive likelihood ratio of 3.33. The area under the curve was 0.83. Conclusion: CD64 seems to be a useful, sensitive, and specific biomarker in discriminating between SIRS and sepsis.

9.
Pesqui. bras. odontopediatria clín. integr ; 18(1): 3870, 15/01/2018. ilus, tab
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-965747

RESUMO

Objective: To assess the relationship between asthma, malocclusion and mouth breathing. Material and Methods: This investigation was a cross-sectional study of 228 children between 6 and 12 years of age, of whom 112 were asthmatic and 116 were not, performed in two Primary Health Units of Porto Alegre, Brazil. The assessment consisted of a mouth exam performed by two calibrated dentists, an interview with parents/caregivers and medical chart data. Mouth breathing was determined through oral-facial changes related to Mouth Breathing Syndrome. Occlusion was assessed according to Angle's Classification for permanent or mixed teeth and regarding primary teeth were based on the canine relationships. The data were assessed by the Chi-square test and Poisson regression, with robust variation, at a p<0.05 significant level. Results: Asthma [PR = 2.12 (95% CI: 1.46-3.08), p<0.001] and the use of pacifiers [PR = 1.98 (95% CI: 1.27-3.07), p<0.001] were associated with mouth breathing, in the final multivariate model. Age [PR = 1.02 (95% CI: 1.00-1.03), p=0.039] and thumb sucking [PR = 1.08 (95% CI: 1.03-1.13), p=0.001] were associated with malocclusion in the final multivariate model, while there was no relationship between asthma and malocclusion (PR = 1.00; 95% CI: 0.94-1.07). Conclusion: This study provides evidence of the relationship between asthma and mouth breathing in children, demonstrating that knowledge regarding the oral health of populations with chronic diseases is fundamental for developing health programmes suitable to their needs and risks.


Assuntos
Humanos , Masculino , Feminino , Criança , Atenção Primária à Saúde , Asma/patologia , Criança , Má Oclusão/diagnóstico , Respiração Bucal/diagnóstico , Brasil , Distribuição de Qui-Quadrado , Estudos Transversais/métodos
10.
Caries Res ; 51(2): 129-135, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28122364

RESUMO

Maltodextrin is a hydrolysate of cornstarch and has been widely used in the food industry associated with sucrose. The addition of starch can increase the cariogenic potential of sucrose; however, there are sparse data regarding the cariogenicity of sucrose associated with maltodextrin. Therefore, the aim of this study was to test in situ if maltodextrin could increase the cariogenic potential of sucrose. This was an in situ, randomized, crossover, split-mouth, and double-blind study. Volunteers wore palatal appliances containing bovine enamel blocks for 2 periods of 14 days. They dripped the following solutions on the enamel blocks 8 times per day: deionized distilled water (DDW), maltodextrin (M), sucrose + maltodextrin (S+M), or sucrose (S). At the end of each experimental period, biofilms were collected and analyzed for microbiological (mutans streptococci, lactobacilli, and total microorganisms counts) and biochemical (calcium, inorganic phosphate, fluoride, and insoluble extracellular polysaccharides concentrations) compositions. The enamel demineralization was assessed by microhardness. Treatments S and S+M resulted in a lower inorganic composition and higher concentration of insoluble extracellular polysaccharides in the biofilms, and higher enamel mineral loss compared to DDW and M. It can be concluded that the cariogenic potential of sucrose is not changed when this carbohydrate is associated with maltodextrin (dextrose equivalent 13-17).


Assuntos
Cárie Dentária/induzido quimicamente , Esmalte Dentário/efeitos dos fármacos , Polissacarídeos/efeitos adversos , Sacarose/efeitos adversos , Edulcorantes/efeitos adversos , Estudos Cross-Over , Método Duplo-Cego , Humanos , Polissacarídeos/administração & dosagem , Sacarose/administração & dosagem , Edulcorantes/administração & dosagem , Adulto Jovem
11.
Folia Primatol (Basel) ; 87(4): 244-261, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27802443

RESUMO

The black lion tamarin, Leontopithecus chrysopygus, is endemic to the Atlantic Forest of the interior of the state of São Paulo in Brazil. Since the discovery of the species, authors have described variations in the pelage of L. chrysopygus, but the nature of this variation has remained unknown. We examined museum specimens and living animals to see if the pelage of L. chrysopygus varies individually, geographically or ontogenetically. We reviewed all known locality records of the species, and concluded that the pelage of L. chrysopygus varies individually and ontogenetically, but not geographically. The extent of the golden chromogenetic fields is larger in younger individuals, and the pelage becomes darker after successive moults. We examined 2 juveniles that presented a pelage similar to adult L. caissara. Geographically, there is a clear break in the phenotypes attributed to L. caissara and L. chrysopygus, precluding clinal variation and corroborating the specific status of the former. The separation between these 2 species is coincident with a tectonic fault known as the 'Guapiara lineament', and may have been caused by a recent activation of this geological formation. We also suggest a historical contact zone between L. chrysopygus and the marmoset Callithrix aurita in the upper Rio Tietê region.


Assuntos
Geografia , Cabelo/anatomia & histologia , Leontopithecus/anatomia & histologia , Pigmentação , Fatores Etários , Animais , Brasil , Feminino , Leontopithecus/classificação , Masculino , Filogenia
12.
Hum Vaccin Immunother ; 12(2): 491-502, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26360663

RESUMO

A single vaccination of Yellow Fever vaccines is believed to confer life-long protection. In this study, results of vaccinees who received a single dose of 17DD-YF immunization followed over 10 y challenge this premise. YF-neutralizing antibodies, subsets of memory T and B cells as well as cytokine-producing lymphocytes were evaluated in groups of adults before (NVday0) and after (PVday30-45, PVyear1-4, PVyear5-9, PVyear10-11, PVyear12-13) 17DD-YF primary vaccination. YF-neutralizing antibodies decrease significantly from PVyear1-4 to PVyear12-13 as compared to PVday30-45, and the seropositivity rates (PRNT≥2.9Log10mIU/mL) become critical (lower than 90%) beyond PVyear5-9. YF-specific memory phenotypes (effector T-cells and classical B-cells) significantly increase at PVday30-45 as compared to naïve baseline. Moreover, these phenotypes tend to decrease at PVyear10-11 as compared to PVday30-45. Decreasing levels of TNF-α(+) and IFN-γ(+) produced by CD4(+) and CD8(+) T-cells along with increasing levels of IL-10(+)CD4(+)T-cells were characteristic of anti-YF response over time. Systems biology profiling represented by hierarchic networks revealed that while the naïve baseline is characterized by independent micro-nets, primary vaccinees displayed an imbricate network with essential role of central and effector CD8(+) memory T-cell responses. Any putative limitations of this cross-sectional study will certainly be answered by the ongoing longitudinal population-based investigation. Overall, our data support the current Brazilian national immunization policy guidelines that recommend one booster dose 10 y after primary 17DD-YF vaccination.


Assuntos
Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Linfócitos B/imunologia , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Vacina contra Febre Amarela/imunologia , Febre Amarela/prevenção & controle , Vírus da Febre Amarela/imunologia , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Brasil , Humanos , Memória Imunológica/imunologia , Interferon gama/sangue , Fator de Necrose Tumoral alfa/sangue , Vacinação , Febre Amarela/virologia
13.
BMC Infect Dis ; 14: 391, 2014 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-25022840

RESUMO

BACKGROUND: The live attenuated 17DD Yellow Fever vaccine is one of the most successful prophylactic interventions for controlling disease expansion ever designed and utilized in larger scale. However, increase on worldwide vaccine demands and manufacturing restrictions urge for more detailed dose sparing studies. The establishment of complementary biomarkers in addition to PRNT and Viremia could support a secure decision-making regarding the use of 17DD YF vaccine subdoses. The present work aimed at comparing the serum chemokine and cytokine kinetics triggered by five subdoses of 17DD YF Vaccine. METHODS: Neutralizing antibody titers, viremia, cytokines and chemokines were tested on blood samples obtained from eligible primary vaccinees. RESULTS AND DISCUSSION: The results demonstrated that a fifty-fold lower dose of 17DD-YF vaccine (587 IU) is able to trigger similar immunogenicity, as evidenced by significant titers of anti-YF PRNT. However, only subdoses as low as 3,013 IU elicit viremia kinetics with an early peak at five days after primary vaccination equivalent to the current dose (27,476 IU), while other subdoses show a distinct, lower in magnitude and later peak at day 6 post-vaccination. Although the subdose of 587 IU is able to trigger equivalent kinetics of IL-8/CXCL-8 and MCP-1/CCL-2, only the subdose of 3,013 IU is able to trigger similar kinetics of MIG/CXCL-9, pro-inflammatory (TNF, IFN-γ and IL-2) and modulatory cytokines (IL-5 and IL-10). CONCLUSIONS: The analysis of serum biomarkers IFN-γ and IL-10, in association to PRNT and viremia, support the recommendation of use of a ten-fold lower subdose (3,013 IU) of 17DD-YF vaccine.


Assuntos
Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Relação Dose-Resposta Imunológica , Vacina contra Febre Amarela/administração & dosagem , Febre Amarela/prevenção & controle , Adolescente , Adulto , Biomarcadores/sangue , Citocinas/sangue , Citometria de Fluxo , Humanos , Cinética , Masculino , Vacinação/métodos , Vacinas Atenuadas/administração & dosagem , Viremia/sangue , Adulto Jovem
14.
São Paulo; s.n; 2014. [74] p. ilus, tab.
Tese em Português | LILACS | ID: biblio-870806

RESUMO

Proteinúria é a principal manifestação da nefrite lúpica (NL) e reflete lesão no podócito. Análise dos biomarcadores do podócito foi realizada com o objetivo de identificar se o fenótipo podocitário é distinto na NL membranosa pura e proliferativa. Expressão de sinaptopodina, proteína 1 do tumor de Wilms (Wilms tumor protein 1 - WT1), proteína epitelial glomerular 1 (glomerular epitelial protein 1 - GLEPP1) e nefrina foi avaliada em 52 biópsias de NL por imunohistoquímica. Expressão preservada de sinaptopodina foi observada em apenas 10 (19,2%) de todas as biópsias enquanto que 42 (80,8%) apresentavam expressão reduzida. Ambos os grupos tinham proteinúria semelhante no momento da biópsia (p = 0,22), porém, no seguimento médio de quatro anos houve uma tendência para menores níveis médios de proteinúria nos pacientes com marcação preservada de sinaptopodina (0,26 ± 0,23 vs 0,84 ± 0,90 g/24 h, p = 0,05) do que naqueles com expressão reduzida. Trinta e nove (75%) biópsias foram classificadas como proliferativa e treze (25%) como membranosa pura. Comparação dos biomarcadores do podócito demonstrou predomíno de marcação preservada de sinaptopodina (69,2%), WT1 (69,2%), GLEPP1 (53,9%) e nefrina (60%) no grupo membranosa pura enquanto apenas < 10% das proliferativas apresentaram expressão preservada. Nossos dados sugerem que nas classes proliferativas parece haver lesão estrutural do podócito, enquanto que na membranosa pura o padrão predominantemente preservado sugere uma lesão funcional do podócito que pode ser responsável pelo melhor prognóstico a longo prazo do desfecho da proteinúria.


Proteinuria is a major feature of lupus nephritis (LN) and reflects podocyte injury. Analysis of podocyte biomarkers was performed attempting to identify if podocyte phenotype is distinct in pure membranous and proliferative LN. Expression of synaptopodin, Wilms tumor protein 1 (WT1), glomerular epithelial protein 1 (GLEPP1) and nephrin was evaluated in 52 LN biopsies by immunohistochemistry. Preserved synaptopodin expression was observed in only 10 (19,2%) of all biopsies while 42 (80,8%) had a reduced expression. Both groups had comparable proteinuria at the time of biopsy (p=0,22), however, in the mean follow-up of four years there was a tendency to lower mean levels of proteinuria in patients with preserved synaptopodin staining (0,26 ± 0,23 vs. 0,84 ± 0,90 g/24 h, p=0,05) than those with diminished expression. Thirty-nine (75%) biopsies were classified as proliferative and thirteen (25%) as pure membranous. Comparison of podocyte biomarkers demonstrated a predominance of preserved staining of synaptopodin (69,2%), WT1 (69,2%), GLEPP1 (53,9%) and nephrin (60%) in the pure membranous group whereas only < 10% of the proliferative showed preserved expression. Our data suggest that in proliferative forms there seems to occur structural podocyte damage, whereas in the pure membranous the predominant preserved pattern suggests a dysfunctional podocyte lesion that may account for the better long-term prognosis of proteinuria outcome.


Assuntos
Lúpus Eritematoso Sistêmico , Nefrite Lúpica , Podócitos , Proteinúria
15.
Dement. neuropsychol ; 7(4)dez. 2013.
Artigo em Inglês | LILACS-Express | ID: lil-696478

RESUMO

Dementia prevalence is increasing in developing countries due to population aging. Brief tests for assessing cognition and activities of daily living are very useful for the diagnosis of dementia by the clinician. Low education, particularly illiteracy, is a hindrance to the diagnosis of dementia in several regions of the world. OBJECTIVES: To compare the Brazilian version of the Cognitive Abilities Screening Instrument-Short Form (CASI-S) with the Mini-Mental State Examination (MMSE) and Pfeffer Functional Activities Questionnaire (PFAQ) for the diagnosis of dementia in illiterate elderly. METHODS: A cross-sectional study with illiterate elderly of both genders seen at the outpatient clinics of the Institute of Gerontology and Geriatrics Jundiaí, São Paulo state was performed. Spearman's correlation coefficient was used to correlate CASI-S, MMSE and PFAQ scores.RESULTS: The sample comprised 29 elderly over 57 years old whose mean scores on the CASI-S (scores ranging from 3 to 23) and the MMSE (scores ranging from 2 to 23) were 11.69 and 12.83, respectively. There was a strong significant correlation between the CASI-S and MMSE (r=0.75, p<0.001) and a moderate correlation coefficient that was significant and negative between the PFAQ and CASI-S (r= -0.53 p=0.003),similar to that between the MMSE and PFAQ (r= -0.41 p=0.025).CONCLUSION: The Brazilian version of the CASI-S demonstrates ease of application and correction in the illiterate elderly, and warrants further studies regarding its applicability for the diagnosis of dementia in populations with a heterogeneous educational background.


A prevalência de demência está aumentando nos países em desenvolvimento, devido ao envelhecimento da população. Testes breves para a avaliação da cognição e atividades de vida diária são muito úteis para o diagnóstico de demência pelo médico. Baixa escolaridade, especialmente analfabetismo, é um obstáculo para o diagnóstico de demência em diversas regiões do mundo. OBJETIVOS: Comparar a versão brasileira do teste Casi-S com o Mini Exame do Estado Mental (MMSE) e Questionário de Atividades Funcionais de Pfeffer (QAFP) para o diagnóstico de demência de analfabetos idosos. MÉTODOS: Estudo de corte transversal, com idosos de ambos os sexos, analfabetos, atendidos no Instituto de Geriatria e Gerontologia do município de Jundiaí, São Paulo. Foi utilizado o coeficiente de correlação de Spearman. RESULTADOS: A amostra foi composta por 29 idosos analfabetos, com médias de escore no Casi-S e no MEEM de 11,69 (variando de 3 a 23 pontos) e 12,83 (variando entre 2 a 23 pontos), respectivamente. Observou-se uma correlação forte e significativa entre o Casi-S e o MEEM (r=0,75; p<0,001) e um coeficiente de correlação moderado, significativo e negativo entre o Casi-S e o QAFP (r= -0,53; p=0,003), o que foi semelhante ao observado entre o MEEM e o QAFP (r= -0.41 p=0.025). Nota-se, portanto, uma correlação importante entre o MEEM e o Casi-S e valores moderados e significativos entre o desempenho cognitivo e o comprometimento nas atividades de vida diária.CONCLUSÃO: Observa-se que o Casi-S é um teste de fácil aplicação e correção, e que merece mais estudos sobre sua aplicabilidade no diagnóstico de demência em populações com heterogeneidade educacional.

16.
Dement Neuropsychol ; 7(4): 410-415, 2013 Oct-Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29213866

RESUMO

Dementia prevalence is increasing in developing countries due to population aging. Brief tests for assessing cognition and activities of daily living are very useful for the diagnosis of dementia by the clinician. Low education, particularly illiteracy, is a hindrance to the diagnosis of dementia in several regions of the world. OBJECTIVES: To compare the Brazilian version of the Cognitive Abilities Screening Instrument-Short Form (CASI-S) with the Mini-Mental State Examination (MMSE) and Pfeffer Functional Activities Questionnaire (PFAQ) for the diagnosis of dementia in illiterate elderly. METHODS: A cross-sectional study with illiterate elderly of both genders seen at the outpatient clinics of the Institute of Gerontology and Geriatrics Jundiaí, São Paulo state was performed. Spearman's correlation coefficient was used to correlate CASI-S, MMSE and PFAQ scores. RESULTS: The sample comprised 29 elderly over 57 years old whose mean scores on the CASI-S (scores ranging from 3 to 23) and the MMSE (scores ranging from 2 to 23) were 11.69 and 12.83, respectively. There was a strong significant correlation between the CASI-S and MMSE (r=0.75, p<0.001) and a moderate correlation coefficient that was significant and negative between the PFAQ and CASI-S (r= -0.53 p=0.003),similar to that between the MMSE and PFAQ (r= -0.41 p=0.025). CONCLUSION: The Brazilian version of the CASI-S demonstrates ease of application and correction in the illiterate elderly, and warrants further studies regarding its applicability for the diagnosis of dementia in populations with a heterogeneous educational background.

17.
Porto Alegre; s.n; 2012. s.p p p.
Tese em Português | Coleciona SUS | ID: biblio-939048

RESUMO

A asma é uma doença inflamatória crônica de alta prevalência na infância e com possíveis repercussões bucais em crianças e adolescentes. O presente trabalho teve como objetivo avaliar a relação existente entre a doença asma e a ocorrência de cárie dentária e defeitos de esmalte. O estudo é caracterizado como observacional analítico transversal e a amostra foi composta por 228 crianças, com idade entre 6 e 12 anos, divididas em um grupo de asmáticas (n= 112) e outro de não asmáticas (n= 116), de duas Unidades Básicas de Saúde de Porto Alegre-RS. A avaliação consistiu de um exame bucal, realizado por dois cirurgiões-dentistas treinados e calibrados (Kappa 0,69 a 1,00), e entrevista estruturada com pais ou responsáveis, além de dados de prontuários. Os dados foram analisados com o auxílio do programa SPSS, utilizando-se os testes Qui-quadrado para as variáveis categóricas e t de Student para variáveis contínuas, para as distribuições normais além da Regressão de Poisson, todos ao nível de significância estatística de p<0,05. Os resultados encontrados nas análises comparando a prevalência da cárie dentária e defeitos de esmalte nas crianças asmáticas e não asmáticas não mostraram diferenças estatisticamente significantes. A análise com as crianças asmáticas, apresentou diferença estatisticamente significante ao relacionar os tipos de medicações utilizadas com a experiência de cárie dentária. A partir do presente estudo pode-se verificar a relação entre a prevalência de cárie dentária e a gravidade da asma conforme os tipos e quantidade de medicações utilizadas. Contudo, não houve relação com o ceo-d/CPOD-D médios e com os defeitos de esmalte quando comparados o grupo de asmáticos e não asmáticos.


Assuntos
Masculino , Feminino , Humanos , Criança , Asma , Cárie Dentária , Esmalte Dentário , Odontologia , Saúde Bucal , Atenção Primária à Saúde , Saúde Pública , Sistema Único de Saúde
18.
JBP, j. bras. odontopediatr. odontol. bebê ; 4(22): 517-21, dez. 2001-jan. 2002. ilus, CD-ROM
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-852079

RESUMO

O objetivo deste trabalho foi realizar uma revisão bibliográfica sobre hipoplasia de esmalte e relatar o tratamento restaurador de uma paciente de dez anos de idade com este tipo de anomalia dentária. Os procedimentos restauradores seguiram os padrões atuais de promoção de saúde bucal, evitando-se o desgaste desnecessário de estrutura dental sadia e empregando-se material restaurador adesivo


Assuntos
Humanos , Feminino , Criança , Hipoplasia do Esmalte Dentário/terapia , Reabilitação Bucal/métodos
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