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1.
Pan Afr Med J ; 33: 289, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31692808

RESUMO

Introduction: This study explores why resuscitation is withheld when mobile emergency medical team arrive at the scene of a cardiac arrest. Methods: We conducted a prospective, observational study in pre hospital emergency services. We included adults' patients, with a suspicion of non-traumatic cardiac arrest (CA) in an out of hospital environment, who received or not cardiopulmonary resuscitation (CPR) by our mobile emergency medical service teams. An analytic study was conducted in order to identify independent factors that could influence the decision to resuscitate OHCA. Results: During study, 228 patients were enrolled, the mean age was 64 +/- 14 years and 59% were men. Eighteen patients (8%) received bystander CPR by witnesses. The median time elapsed to arrive at the scene was 13 [8-25] min. The median "noflow" was 22 [10-34] min. The resuscitation decision was taken by the mobile EMS staff for 106 patients (46.5%). For other patients, the decision not to resuscitate was motivated solely by the finding of a confirmed state of death in an elderly patient (p = 0.045). The predictive decision factor for resuscitation was the no flow time less than 18.5 min, Odds Ratio adjusted with 95% confidence interval to: 1.38 (1.24 - 3.55) (p <0.001). Overall out of hospital survival rate was 17% of resuscitated patients. Conclusion: The decision to resuscitate a cardiac arrest outside of the hospital depends more on the "no flow" time than on the presumed etiologies.


Assuntos
Reanimação Cardiopulmonar/métodos , Tomada de Decisões , Serviços Médicos de Emergência/estatística & dados numéricos , Parada Cardíaca Extra-Hospitalar/terapia , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Taxa de Sobrevida , Fatores de Tempo
2.
Acta Biochim Pol ; 63(3): 581-7, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27382647

RESUMO

As part of the contribution to the global efforts in research of thermostable enzymes being of industrial interest, we focus on the isolation of thermophilic bacteria from Tunisian hot springs. Among the collection of 161 strains of thermophilic Bacillus isolated from different samples of thermal water in Tunisia, 20% are capable of growing at 100°C and the rest grow at 70°C or above. Preliminary activity tests on media supplemented with enzyme-substrates confirmed that 35 strains produced amylases, 37 - proteases, 43 - cellulases, 31 - xylanases and 37 - mannanases. The study of the effect of temperature on enzyme activity led to determination of the optimal temperatures of activities that vary between 60 and 100°C. Several enzymes were active at high temperatures (80, 90 and 100°C) and kept their activity even at 110°C. Several isolated strains producing enzymes with high optimal temperatures of activity were described for the first time in this study. Both strains B62 and B120 are producers of amylase, protease, cellulase, xylanase, and mannanase. The sequencing of 16S DNA identified isolated strains as Geobacillus kaustophillus, Aeribacillus pallidus, Geobacillus galactosidasus and Geobacillus toebii.


Assuntos
Bacillus/enzimologia , Proteínas de Bactérias/biossíntese , Geobacillus/enzimologia , Amilases/biossíntese , Amilases/química , Bacillus/genética , Proteínas de Bactérias/química , Celulase/biossíntese , Celulase/química , Endopeptidases/biossíntese , Endopeptidases/química , Estabilidade Enzimática , Geobacillus/genética , Fontes Termais/microbiologia , Temperatura Alta , Tipagem Molecular , Filogenia , RNA Ribossômico 16S , Tunísia , Microbiologia da Água , Xilosidases/biossíntese , Xilosidases/química
3.
Biomed Res Int ; 2016: 9178962, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27069928

RESUMO

A new thermostable, haloalkaline, solvent stable SDS-induced serine protease was purified and characterized from a thermophilic Geobacillus toebii LBT 77 newly isolated from a Tunisian hot spring. This study reveals the potential of the protease from Geobacillus toebii LBT 77 as an additive to detergent with spectacular proprieties described for the first time. The protease was purified to homogeneity by ammonium sulfate precipitation followed by Sephadex G-75 and DEAE-Cellulose chromatography. It was a monomeric enzyme with molecular weight of 30 kDa. The optimum pH, temperature, and NaCl for maximum protease activity were 13.0, 95°C, and 30%, respectively. Activity was stimulated by Ca(2+), Mg(2+), DTNB, ß-mercaptoethanol, and SDS. The protease was extremely stable even at pH 13.25, 90°C, and 30% NaCl and in the presence of hydrophilic, hydrophobic solvents at high concentrations. The high compatibility with ionic, nonionic, and commercial detergents confirms the utility as an additive to cleaning products. Kinetic and thermodynamic characterization of protease revealed K m = 1 mg mL(-1), V max = 217.5 U mL(-1), K cat/K m = 99 mg mL(-1) S(-1), E a = 51.5 kJ mol(-1), and ΔG (⁎) = 56.5 kJ mol(-1).


Assuntos
Proteínas de Bactérias/química , Proteínas de Bactérias/isolamento & purificação , Geobacillus/enzimologia , Serina Proteases/química , Serina Proteases/isolamento & purificação , Proteínas de Bactérias/metabolismo , Estabilidade Enzimática , Temperatura Alta , Compostos Orgânicos , Serina Proteases/metabolismo , Tensoativos
4.
Therapie ; 63(1): 43-7, 2008.
Artigo em Francês | MEDLINE | ID: mdl-18387275

RESUMO

Local Bacillus Calmette-Guerin (BCG) immunotherapy is an effective and widely used treatment for superficial bladder carcinoma. Local side effects are frequent, whereas systemic side effects are rare, but more serious. We report four cases of systemic BCG reaction. Although uncommon, this infectious complication of BCG therapy should always be considered in the appropriate clinical setting. The best approach to minimize this complication is a strict compliance with precautions and a close and rigorous surveillance of this drug.


Assuntos
Adjuvantes Imunológicos/efeitos adversos , Adjuvantes Imunológicos/uso terapêutico , Vacina BCG/efeitos adversos , Vacina BCG/uso terapêutico , Adulto , Idoso , Vacina BCG/administração & dosagem , Humanos , Masculino , Neoplasias da Bexiga Urinária/tratamento farmacológico
5.
Therapie ; 63(1): 43-7, 2008.
Artigo em Francês | MEDLINE | ID: mdl-27392997

RESUMO

Local Bacillus Calmette-Guerin (BCG) immunotherapy is an effective and widely used treatment for superficial bladder carcinoma. Local side effects are frequent, whereas systemic side effects are rare, but more serious. We report four cases of systemic BCG reaction. Although uncommon, this infectious complication of BCG therapy should always be considered in the appropriate clinical setting. The best approach to minimize this complication is a strict compliance with precautions and a close and rigorous surveillance of this drug.

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