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1.
Support Care Cancer ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31773272

RESUMO

PURPOSE: The objective of this multicentre study was to verify the relationship between the scores of quality of life (QoL) and the decayed, missing and filled teeth (DMFT) and radiation caries (RC) in patients treated with radiation therapy (RT) for head and neck cancer, and through this to determine if RC is capable of causing a significant decrease in the QoL. METHODS: One hundred patients were divided into 2 groups: patients with at least 1 year of RT completion who developed RC (study group, n = 50); and patients with at least 1 year of RT completion who did not develop RC (control group, n = 50). All patients answered the Brazilian-Portuguese version of the University of Washington quality of life (UW-QoL) questionnaire, which was divided into physical and social-emotional functioning domains and evaluated the DMFT index score. RESULTS: The mean score of QoL was 927.2 in the control group and 878.1 in the study group (P = 0.24). The mean score of DMFT was 30.5 in the study group and 20.7 in the control group (P = 0.001). The items recreation and saliva, which belong to the physical function domain, showed a statistically significant difference between the study and control groups (P = 0.031 and P = 0.047, respectively). Saliva was the item with the higher number of patient complaints in both groups. CONCLUSION: RC had a negative impact on the QoL of HNC patients.

2.
Cien Saude Colet ; 24(9): 3529-3537, 2019 Sep 09.
Artigo em Português | MEDLINE | ID: mdl-31508770

RESUMO

Caries Assessment Spectrum and Treatment (CAST) is an instrument developed for caries detection to be used in epidemiological surveys; it has been validated and is believed to provide a clearer picture of the oral health status than the criteria provided by the World Health Organization (WHO). This article aims to compare the epidemiological survey results using the CAST instrument and the WHO criteria in the same population. 680 schoolchildren aged 6-8 years from Federal District, Brazil, were evaluated by two examiners trained to use the CAST. The visible plaque index (VPI) and gingival bleeding index (GBI) were also evaluated. The maximum CAST codes per tooth were converted into the dmf/DMF, the mean scores for primary and permanent dentition were calculated. The mean age was 7.45(± 0.91) years. The prevalence of caries differed when CAST and the WHO criteria were applied. In the primary dentition, it was 65.44% and 61.61%, and for the permanent dentition, 38.19% and 10.2%, respectively. It was possible to calculate the mean dmft [2.4(± 2.7)] and the DMFT [0.16(± 0.53)] using CAST. VPI was associated with higher maximum CAST scores p < 0,005. The way CAST results are presented showed a higher sensibility to identify the presence and severity of carious lesions in comparison to the WHO criteria.

3.
Ciênc. Saúde Colet ; 24(9): 3529-3537, set. 2019. tab, graf
Artigo em Português | LILACS-Express | ID: biblio-1019677

RESUMO

Resumo O CAST (Caries Assessment Spectrum and Treatment) é um instrumento desenvolvido para a detecção de cárie a ser utilizado em levantamentos epidemiológicos. Foi validado e tem se mostrado efetivo, fornecendo um diagnóstico mais preciso do estado de saúde bucal do que o critério OMS, recomendado pela Organização Mundial da Saúde. O objetivo deste artigo é comparar a apresentação dos resultados de cárie dentária utilizando o instrumento CAST e o critério OMS, numa mesma população. Foram avaliados por dois examinadores treinados na utilização do instrumento CAST 680 escolares de 6 a 8 anos do Distrito Federal, Brasil. A avaliação constou do índice de placa visível (IPV) e do índice de sangramento gengival (ISG). Os escores CAST dente foram convertidos em componentes ceo/CPO e calculados os ceod/CPOD. Os pais responderam a um questionário sociodemográfico. A idade média foi 7,45 anos (± 0,91). A prevalência de cárie na dentição decídua foi de 65,44% e 61,61%, considerando o CAST e o critério da OMS, respectivamente; na dentição permanente: 38,19% e 10,2%, respectivamente. A média do ceod foi de 2.4 (± 2.7) e a média do CPOD 0.16 (± 0.53). o IPV foi associado a maiores CAST máximos p < 0,005. O instrumento CAST demonstrou maior sensibilidade em identificar a presença e gravidade de lesões cariosas quando comparado ao critério OMS.


Abstract Caries Assessment Spectrum and Treatment (CAST) is an instrument developed for caries detection to be used in epidemiological surveys; it has been validated and is believed to provide a clearer picture of the oral health status than the criteria provided by the World Health Organization (WHO). This article aims to compare the epidemiological survey results using the CAST instrument and the WHO criteria in the same population. 680 schoolchildren aged 6-8 years from Federal District, Brazil, were evaluated by two examiners trained to use the CAST. The visible plaque index (VPI) and gingival bleeding index (GBI) were also evaluated. The maximum CAST codes per tooth were converted into the dmf/DMF, the mean scores for primary and permanent dentition were calculated. The mean age was 7.45(± 0.91) years. The prevalence of caries differed when CAST and the WHO criteria were applied. In the primary dentition, it was 65.44% and 61.61%, and for the permanent dentition, 38.19% and 10.2%, respectively. It was possible to calculate the mean dmft [2.4(± 2.7)] and the DMFT [0.16(± 0.53)] using CAST. VPI was associated with higher maximum CAST scores p < 0,005. The way CAST results are presented showed a higher sensibility to identify the presence and severity of carious lesions in comparison to the WHO criteria.

4.
Clin Oral Investig ; 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31372829

RESUMO

OBJECTIVES: This study assessed the human pulp response after adhesive restoration of cavities by indirect pulp capping with a conventional or a resin-modified glass ionomer cement. MATERIALS AND METHODS: Deep cavities prepared in 26 human premolars were lined with Riva Light Cure (Riva LC), Riva Self Cure (Riva SC), or Dycal, and then restored with composite resin. Four teeth were used as intact control. After time intervals of 7 or 30 days, the teeth were extracted, processed for histological evaluation of the pulp, and the remaining dentin thickness (RDT) between the cavity floor and the pulp was measured. RESULTS: At 7 days, a slight pulp inflammation associated with discrete tissue disorganization was observed in most of t the teeth lined with Riva LC and Riva SC. Moderate pulp inflammation occurred in one tooth lined with Riva LC. Bacteria were identified in one specimen of the same group that exhibited no pulp damage. At 30 days, slight pulp inflammation and discrete tissue disorganization persisted in two specimens treated with Riva LC, in which a thin layer of tertiary dentin was deposited. Mean RDTs ranged from 383.0 to 447.8 µm. CONCLUSIONS: Riva LC produced more damage to the pulp than Riva SC. However, the initial pulp damage decreased over time and after 30 days both GICs were labeled as biocompatible. CLINICAL RELEVANCE: In this study conducted with human teeth, the conventional and the resin-modified glass ionomer cements investigated were shown not to cause post-operative sensitivity or persistent pulp damage when applied as liners in very deep cavities, thereby indicating their biocompatibility.

5.
Int J Paediatr Dent ; 29(4): 422-428, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30803096

RESUMO

BACKGROUND: Dental caries diagnosis and management have changed over time. AIM: To identify the treatment decision-making process performed by paediatric dentists, after caries detection using CAST instrument (Caries Assessment Spectrum and Treatment), for enamel (CAST 3) and non-cavitated dentin caries lesions (CAST 4) before and after analysing radiographic images. DESIGN: Seventy-four paediatric dentists were invited to participate. Twelve clinical cases were presented online, and treatment decisions before and after the analysis of bitewing radiographs were evaluated. RESULTS: Sixty-one specialists answered the questionnaire. The necessity for radiographs was significantly associated with the presence and depth of the caries lesion (P < 0.0001). CAST 3 lesions were preferentially monitored before and after the radiographic assessment. For CAST 4 lesions limited to the outer half of dentin, treatments indicated before and after radiographic analysis were sealant (33%) and restoration (40%). For the lesions in the inner half of dentin, restoration was the most cited before (45%) and after (84%) radiographs. The radiographic depth was the only significant independent variable when "change in the treatment option" was analysed by the regression model (P < 0.001). CONCLUSIONS: Enamel lesions were treated through less invasive treatments. Radiographs influenced the decision, especially for the lesions that involved the inner half of dentin.


Assuntos
Cárie Dentária , Criança , Esmalte Dentário , Restauração Dentária Permanente , Dentina , Humanos , Dente Decíduo
6.
J Public Health Dent ; 78(4): 306-312, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29752807

RESUMO

OBJECTIVES: To evaluate whether an association exists between school performance, quality of life, dental caries, and dental pain in children between 6 and 8 years of age. METHODS: All 6- to 8-year-old children enrolled in the second year of the six public schools in Paranoá-FD were invited to participate in this cross-sectional study. In total, 374 consented. Dental caries was recorded according to the Caries Assessment Spectrum and Treatment (CAST) index by two trained examiners. Dental pain was assessed during clinical examination. The Child Perceptions Questionnaire (CPQ) was used to assess the oral health-related quality of life, and sociodemographic data were collected. The child's school performance was measured through Provinha Brasil according to the regulations of the Ministry of Education. Descriptive statistics, bivariate analysis, and a multivariate regression model were used for data analysis. RESULTS: 185 boys and 189 girls participants with a mean age of 7.2 (±0.5) years were included in the study. The prevalence of dentin carious lesions for this group was 40.6 and 49.2 percent, including enamel-only lesions. The prevalence of dental pain was 2.8 percent. A high performance in reading (63.6 percent) and in mathematics (79.4 percent) was observed and were not associated with the presence of cavitated dentin carious lesions or dental pain. A significant association was found between school performance and the final CPQ score. CONCLUSION: School performance as measured by the Provinha Brasil examination was not associated with carious lesions or dental pain. Students with lower school performance had higher CPQ scores.


Assuntos
Cárie Dentária , Saúde Bucal , Brasil , Criança , Estudos Transversais , Índice CPO , Feminino , Humanos , Masculino , Prevalência , Qualidade de Vida
7.
BMC Oral Health ; 18(1): 65, 2018 04 18.
Artigo em Inglês | MEDLINE | ID: mdl-29669561

RESUMO

BACKGROUND: This study aimed to evaluate the survival rate of glass hybrid restorations placed under the atraumatic restorative treatment (ART) technique in first permanent molars affected by molar incisor hypomineralization (MIH). METHODS: Sixty teeth with severe MIH associated to carious dentin lesions without pulp involvement were included. Treatments were performed by one trained dentist using the ART approach and restored with a glass hybrid restorative system (Equia Forte, GC®) on school premises. Treatments were evaluated after 6 and 12 months by an independent examiner using the modified ART criterion. Data analysis involved descriptive statistics and actuarial success analysis. RESULTS: The sample comprised 24 (54.54%) girls and 20 (45.45%) boys with a mean age of 10.55 (±1.25) years. In regard to the number of surfaces involved in the restorations, 29 (48.3%) comprised one surface and 31 (51.7%) two or more surfaces. Considering cavity extent, 25 (41%) presented dentin cavitation without cusp weakness, 23 (37.7%) with large dentin cavitation with cusp weakness and 13 (21.3%) with large dentin cavitation with the breakdown of one or more cusps. Only 4 teeth required local anesthesia. A success rate of 98.3% after 6 and 12 months was observed, as only one restoration failed. The only failure occurred in a restoration involving three or more sur-faces presenting the breakdown of all cusps. CONCLUSION: Restorations using a glass hybrid restorative system and performed in the field with the ART technique proved, after 12 months of evaluation, to be an effective approach to preserving first permanent molars affected by MIH. TRIAL REGISTRATION: REBEC-RBR-8drccq (17/06/15).


Assuntos
Tratamento Dentário Restaurador sem Trauma , Hipoplasia do Esmalte Dentário/terapia , Cimentos de Ionômeros de Vidro/uso terapêutico , Adolescente , Criança , Tratamento Dentário Restaurador sem Trauma/métodos , Cárie Dentária/terapia , Feminino , Humanos , Masculino , Resultado do Tratamento
8.
Pediatr Dent ; 40(1): 12-17, 2018 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-29482676

RESUMO

PURPOSE: The purpose of this systematic review was to verify whether child and adolescent oral health affected academic performance. METHODS: A literature search conducted in March 2017 on PubMed, Lilacs, Web of Science, and Scopus databases identified 2,009 papers, six of which were included in the final review. Quality appraisal and risk of bias were evaluated using the quality assessment tool for observational cohort and cross-sectional studies. RESULTS: Two papers were classified as being of good quality, one as fair, and three as poor. In four publications, oral health conditions were measured by taking only dental caries into account, while in two others treatment needs and dental trauma were also considered. Although four papers concluded that children's academic performance and poor oral health were associated, the results were not considered reliable because of the high risk of bias. The two papers classified as being of good quality did not show an association between oral health and academic performance, unless mediated by socioeconomic factors. CONCLUSION: Further well-designed studies are required to demonstrate whether children's oral health can have a negative influence on their academic performance.


Assuntos
Desempenho Acadêmico , Saúde Bucal , Odontopatias/complicações , Adolescente , Criança , Cárie Dentária/complicações , Hemorragia Gengival/complicações , Humanos , Má Oclusão/complicações , Fatores Socioeconômicos , Traumatismos Dentários/complicações
9.
Lasers Med Sci ; 33(4): 709-717, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29134404

RESUMO

The purpose of this study was to evaluate the effectiveness of anti-microbial photodynamic therapy (aPDT) mediated by curcumin (Cur) associated with LED light against biofilms of Candida dubliniensis, and further, investigate cellular uptake and drug penetration through the biofilms under confocal laser scanning microscopy (CLSM). Four C. dubliniensis strains were tested: three clinical isolates from HIV-positive patients and one reference strain (CBS 7987). Biofilms were treated with three Cur concentrations (20.0, 30.0, and 40.0 µM). All samples were incubated in the dark for 20 min and exposed to a 5.28 J/cm2 of LED light fluence. Additional samples of each strain were treated either with Cur or LED light only. Control samples had neither Cur nor light. After aPDT, results were read using the XTT salt reduction method. The data were statistically analyzed by two-way ANOVA followed by Games-Howell post-hoc test (α = 0.05). Confocal laser scanning microscopy was used to verify both the uptake of Cur by yeast cells and its penetration through the biofilm. The results showed that aPDT promoted significant reduction on the metabolism of the biofilm-organized cells of C. dubliniensis. Further, while Cur was rapidly taken up by C. dubliniensis cells, a longer time interval was required to allow Cur penetration into biofilm cells. Based on these results, aPDT associating LED and Cur presents promising potential on fungal control of biofilms of C. dubliniensis.


Assuntos
Anti-Infecciosos/farmacologia , Biofilmes/efeitos dos fármacos , Candida/fisiologia , Curcumina/farmacologia , Fotoquimioterapia , Candida/efeitos dos fármacos , Candida/isolamento & purificação , Contagem de Colônia Microbiana , Humanos , Microscopia Confocal , Plâncton/efeitos dos fármacos
10.
Caries Res ; 51(5): 500-506, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28968606

RESUMO

Caries detection is fundamental to understanding the oral health status of a population and is the basis for caries diagnosis for individual patients. Although different caries detection/diagnosis criteria are available, none of them include the total spectrum of dental caries (which ranges from a sound tooth to a tooth lost due to caries) other than the Caries Assessment Spectrum and Treatment (CAST) instrument. The CAST codes and descriptions were submitted to experienced epidemiologists from across the world for obtaining face and content validity. Its construct validity and reproducibility under field conditions were tested in child and adult populations, and showed a high level of agreement between examiners. Compared to what is usually reported in the literature, CAST provides more relevant information on caries prevalence, experience, and severity. CAST is straightforward and easy to use. A manual with valuable information about how to apply CAST and report its results has been published in order to facilitate communication among researchers, oral health planners, and medical professionals. Feedback from researchers indicates that CAST is considered an asset and that reporting results after using CAST is uncomplicated. More research about the effects of CAST in different cultures and age groups is required.


Assuntos
Cárie Dentária/diagnóstico , Cárie Dentária/epidemiologia , Inquéritos de Saúde Bucal/métodos , Saúde Global , Humanos , Prevalência , Reprodutibilidade dos Testes , Organização Mundial da Saúde
11.
J Craniomaxillofac Surg ; 45(3): 364-370, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28169044

RESUMO

Although ozone therapy is extensively applied when wound repair and antimicrobial effect are necessary, little is known about cellular mechanisms regarding this process. Thus, this study aimed to evaluate ozone cytotoxicity in fibroblasts (L929) and keratinocytes (HaCaT) cell lines, its effects on cell migration and its antimicrobial activity. Cells were treated with ozonated phosphate-buffered saline (8, 4, 2, 1, 0.5 and 0.25 µg/mL ozone), chlorhexidine 0.2% or buffered-solution, and cell viability was determined through MTT assay. The effect of ozone on cell migration was evaluated through scratch wound healing and transwell migration assays. The minimum inhibitory concentrations for Candida albicans and Staphylococcus aureus were determined. Ozone showed no cytotoxicity for the cell lines, while chlorhexidine markedly reduced cell viability. Although no significant difference between control and ozone-treated cells was observed in the scratch assay, a considerable increase in fibroblasts migration was noticed on cells treated with 8 µg/mL ozonated solution. Ozone alone did not inhibit growth of microorganisms; however, its association with chlorhexidine resulted in antimicrobial activity. This study confirms the wound healing and antimicrobial potential of ozone therapy and presents the need for studies to elucidate the molecular mechanisms through which it exerts such biological effects.


Assuntos
Anti-Infecciosos/farmacologia , Testes de Sensibilidade Microbiana , Ozônio/farmacologia , Cicatrização/efeitos dos fármacos , Anti-Infecciosos/uso terapêutico , Candida albicans/efeitos dos fármacos , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Fibroblastos/citologia , Humanos , Técnicas In Vitro , Queratinócitos/citologia , Testes de Sensibilidade Microbiana/métodos , Ozônio/uso terapêutico , Staphylococcus aureus/efeitos dos fármacos
12.
Pediatr Dent ; 39(7): 445-449, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-29335050

RESUMO

PURPOSE: The purpose of this study was to evaluate the prevalence of and relationship between hypomineralized second primary molars (HSPM) and hypomineralized primary canines (HPC) with molar-incisor hypomineralization (MIH) in 1,963 schoolchildren. METHODS: The European Academy of Paediatric Dentistry (EAPD) criterion was used for scoring HSPM/HPC and MIH. Only children with four permanent first molars and eight incisors were considered in calculating MIH prevalence (n equals 858); for HSPM/HPC prevalence, only children with four primary second molars (n equals 1,590) and four primary canines (n equals 1,442) were considered. To evaluate the relationship between MIH/HSPM, only children meeting both criteria cited were considered (n equals 534), as was true of MIH/HPC (n equals 408) and HSPM/HPC (n equals 360; chi-square test and logistic regression). RESULTS: The prevalence of MIH was 14.69 percent (126 of 858 children). For HSPM and HPC, the prevalence was 6.48 percent (103 of 1,592) and 2.22 percent (32 of 1,442), respectively. A significant relationship was observed between MIH and both HSPM/HPC (P<0.001). The odds ratio for MIH based on HSPM was 6.31 (95 percent confidence interval [CI] equals 2.59 to 15.13) and for HPC was 6.02 (95 percent CI equals 1.08 to 33.05). CONCLUSION: The results led to the conclusion that both hypomineralized second primary molars and hypomineralized primary canines are associated with molar-incisor hypomineralization, because children with HSPM/HPC are six times more likely to develop MIH.


Assuntos
Hipoplasia do Esmalte Dentário/epidemiologia , Incisivo , Dente Molar , Criança , Estudos Transversais , Feminino , Humanos , Masculino , Prevalência , Dente Decíduo
13.
Int J Paediatr Dent ; 27(5): 372-379, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27748991

RESUMO

BACKGROUND: Very little is known about how patients perceive molar-incisor hypomineralization (MIH). AIM: The aim of this study was to identify whether parents and children perceive opacities associated with MIH as an oral health problem. DESIGN: A case-control study was designed in which 131 children diagnosed with MIH were matched by age, sex, and school to 131 children without MIH. The Child and Parent Questionnaire about Tooth Appearance was used to assess reports from parents, who were interviewed by telephone, and children, who were interviewed on school premises. The Spearman correlation and Wilcoxon and Fisher's exact tests were used to assess the data. RESULTS: For the first part of the questionnaire, children avoided smiling because of the appearance of their teeth, and mothers seemed to be distressed by the appearance of their children's teeth (P < 0.05). For the second part, children showed dissatisfaction with their 'tooth alignment' and 'tooth discoloration' compared with the controls, while mothers were not satisfied with their children's tooth discoloration in comparison with controls (P < 0.05). Children and mothers were concerned about tooth discoloration (P < 0.05). CONCLUSION: Poor agreement was found between the reports of children and their mothers, but both children and mothers in the MIH group perceived MIH opacities negatively in comparison with controls.


Assuntos
Hipoplasia do Esmalte Dentário , Saúde Bucal , Pais/psicologia , Percepção , Psicologia da Criança , Adolescente , Brasil , Estudos de Casos e Controles , Criança , Feminino , Humanos , Entrevistas como Assunto , Masculino , Inquéritos e Questionários , Descoloração de Dente
14.
Braz Dent J ; 26(2): 160-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25831108

RESUMO

This in vitro study evaluated in fibroblast cultures the direct cytotoxicity of universal, self-etching and etch-and-rinse adhesive systems according to the polymerization time. Paper discs were impregnated with adhesives and light-cured (10, 20 or 40 s). The discs were then immersed in culture medium to obtain the eluates for the experimental groups (A1-Single Bond 2; A2-Scotchbond Multi-purpose; A3-Clearfil SE Bond; A4 Scotchbond Universal). As a negative control, paper discs were immersed in culture medium only. After 24 h or 7 days, the eluate obtained was applied on fibroblast culture. Cell viability, cell morphology, membrane damage and the presence of residual monomers were evaluated by MTT assay, SEM, flow cytometry and high-performance liquid chromatography (HPLC), respectively. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (=0.05). All adhesive systems significantly reduced 33-51% cell metabolism when compared to the negative control, regardless of polymerization time, storage period and adhesive system. Moreover, the adhesives caused intense morphological alterations and cell membrane damage. Toxicity was directly related to the presence of residual monomers in the eluates. Residual monomers and additional components are capable of reducing mitochondrial activity, causing morphological alterations and disruption of the cell membrane in fibroblasts, regardless of the polymerization time. This study highlights that despite the more complex composition of the universal adhesive system, its biological response was not more toxic when compared with other systems, even when the shortest polymerization time was tested in cell culture.


Assuntos
Corrosão Dentária/métodos , Adesivos Dentinários/toxicidade , Fibroblastos/efeitos dos fármacos , Bis-Fenol A-Glicidil Metacrilato , Cromatografia Líquida de Alta Pressão , Citometria de Fluxo , Técnicas In Vitro , Cura Luminosa de Adesivos Dentários , Microscopia Eletrônica de Varredura , Polimerização , Cimentos de Resina , Propriedades de Superfície , Fatores de Tempo
15.
Braz. dent. j ; 26(2): 160-168, Mar-Apr/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-741210

RESUMO

This in vitro study evaluated in fibroblast cultures the direct cytotoxicity of universal, self-etching and etch-and-rinse adhesive systems according to the polymerization time. Paper discs were impregnated with adhesives and light-cured (10, 20 or 40 s). The discs were then immersed in culture medium to obtain the eluates for the experimental groups (A1-Single Bond 2; A2-Scotchbond Multi-purpose; A3-Clearfil SE Bond; A4 Scotchbond Universal). As a negative control, paper discs were immersed in culture medium only. After 24 h or 7 days, the eluate obtained was applied on fibroblast culture. Cell viability, cell morphology, membrane damage and the presence of residual monomers were evaluated by MTT assay, SEM, flow cytometry and high-performance liquid chromatography (HPLC), respectively. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (=0.05). All adhesive systems significantly reduced 33-51% cell metabolism when compared to the negative control, regardless of polymerization time, storage period and adhesive system. Moreover, the adhesives caused intense morphological alterations and cell membrane damage. Toxicity was directly related to the presence of residual monomers in the eluates. Residual monomers and additional components are capable of reducing mitochondrial activity, causing morphological alterations and disruption of the cell membrane in fibroblasts, regardless of the polymerization time. This study highlights that despite the more complex composition of the universal adhesive system, its biological response was not more toxic when compared with other systems, even when the shortest polymerization time was tested in cell culture.


O presente estudo in vitro avaliou a citotoxicidade direta dos sistemas adesivos convencionais, autocondicionantes e universais de acordo com o tempo de polimerização em cultura de fibroblastos. Discos de papel foram impregnados com adesivos e fotoativados (10, 20 e 40 s). Os discos foram posteriormente imersos em meio de cultura para obtenção dos eluatos dos grupos experimentais (A1-Single Bond 2; A2-Scotchbond Multi-purpose; A3-Clearfil SE Bond; A4 Scotchbond Universal). Para o controle negativo, os discos de papel foram imersos somente em meio de cultura. Após 24 h ou 7 dias, o eluato obtido foi aplicado na cultura de fibroblastos. O metabolismo celular, morfologia, dano de membrana e presença de monômeros residuais foram avaliados por teste de MTT, MEV, citometria de fluxo e HPLC, respectivamente. Os dados foram analisados estatisticamente por Kruskal-Wallis e Mann-Whitney. Todos os sistemas adesivos reduziram significativamente o metabolismo celular em 33 a 51% quando comparados ao grupo controle, independente do tempo de polimerização, período de armazenamento e tipo de sistema adesivo. O eluato do adesivos causou ainda intensas alterações morfológicas e danos à membrana celular. A toxicidade foi diretamente relacionada à presença de monômeros residuais nos eluatos experimentais. Monômeros residuais e componentes adicionais dos sistemas adesivos foram capazes de reduzir a atividade mitocondrial, causar alterações morfológicas e danos à membrana citoplasmática de fibroblastos, independente do tempo de polimerização. Esse estudo evidencia que apesar de uma composição mais complexa do sistema adesivo universal, sua resposta biológica não apresentou maior toxicidade quando comparado aos demais sistemas, mesmo no menor tempo de polimerização quando testados em cultura celular.


Assuntos
Corrosão Dentária/métodos , Adesivos Dentinários/toxicidade , Fibroblastos/efeitos dos fármacos , Bis-Fenol A-Glicidil Metacrilato , Cromatografia Líquida de Alta Pressão , Citometria de Fluxo , Técnicas In Vitro , Cura Luminosa de Adesivos Dentários , Microscopia Eletrônica de Varredura , Polimerização , Cimentos de Resina , Propriedades de Superfície , Fatores de Tempo
16.
J Dent ; 43(5): 537-45, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25748671

RESUMO

OBJECTIVES: To evaluate the short-term response of human pulps to ethanol-wet bonding technique. METHODS: Deep class V cavities were prepared on 17 sound premolars and divided into three groups. After acid-etching, the cavities from groups 1 (G1) and 2 (G2) were filled with 100% ethanol or distilled water, respectively, for 60 s before the application of Single Bond 2. In group 3 (G3, control), the cavity floor was lined with calcium hydroxide before etching and bonding. All cavities were restored with resin composite. Two teeth were used as intact control. The teeth were extracted 48h after the clinical procedures. From each tooth serial sections were obtained and stained with haematoxylin and eosin (H/E) and Masson's trichrome. Bacteria microleakage was assessed using Brown & Brenn. All sections were blindly evaluated for five histological features. RESULTS: Mean remaining dentine thickness was 463±65µm (G1); 425±184µm (G2); and 348±194µm (G3). Similar pulp reactions followed ethanol- or water-wet bonding techniques. Slight inflammatory responses and disruption of the odontoblast layer related to the cavity floor were seen in all groups. Stained bacteria were not detected in any cavities. Normal pulp tissue was observed in G3 except for one case. CONCLUSIONS: After 48h, ethanol-wet bonding does not increase pulpal damage compared to water-wet bonding technique. CLINICAL SIGNIFICANCE: Ethanol-wet bonding may increase resin-dentine bond durability. This study reported the in vivo response of human pulp tissue when 100% ethanol was applied previously to an etch-and-rinse simplified adhesive system.


Assuntos
Colagem Dentária/métodos , Preparo da Cavidade Dentária , Polpa Dentária/efeitos dos fármacos , Adesivos Dentinários/farmacologia , Etanol/farmacologia , Adolescente , Dente Pré-Molar , Materiais Biocompatíveis/uso terapêutico , Forramento da Cavidade Dentária , Infiltração Dentária/microbiologia , Polpa Dentária/microbiologia , Polpa Dentária/patologia , Polpa Dentária/ultraestrutura , Exposição da Polpa Dentária/terapia , Dentina/microbiologia , Dentina/ultraestrutura , Adesivos Dentinários/química , Etanol/química , Humanos , Odontoblastos/patologia
17.
J Adhes Dent ; 17(2): 155-61, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25763410

RESUMO

PURPOSE: To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. MATERIALS AND METHODS: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (a = 5%). RESULTS: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. CONCLUSION: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred.


Assuntos
Polpa Dentária/efeitos dos fármacos , Restauração Dentária Permanente/métodos , Peróxido de Hidrogênio/toxicidade , Clareadores Dentários/toxicidade , Clareamento Dental/métodos , Ataque Ácido Dentário/métodos , Animais , Bovinos , Técnicas de Cultura de Células , Linhagem Celular , Forma Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Resinas Compostas/química , Meios de Cultura , Esmalte Dentário/efeitos dos fármacos , Materiais Dentários/química , Polpa Dentária/citologia , Dentina/efeitos dos fármacos , Cura Luminosa de Adesivos Dentários , Camundongos , Odontoblastos/efeitos dos fármacos , Temperatura Ambiente , Fatores de Tempo
18.
Lasers Med Sci ; 30(2): 549-59, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23748800

RESUMO

Antimicrobial photodynamic therapy represents an alternative method of killing resistant pathogens. Efforts have been made to develop delivery systems for hydrophobic drugs to improve the photokilling. This study evaluated the photodynamic effect of chloro-aluminum phthalocyanine (ClAlPc) encapsulated in nanoemulsions (NE) on methicillin-susceptible and methicillin-resistant Staphylococcus aureus suspensions and biofilms. Suspensions and biofilms were treated with different delivery systems containing ClAlPc. After the pre-incubation period, the drug was washed-out and irradiation was performed with LED source (660 ± 3 nm). Negative control samples were not exposed to ClAlPc or light. For the suspensions, colonies were counted (colony-forming units per milliliter (CFU/mL)). The metabolic activity of S. aureus suspensions and biofilms were evaluated by the XTT assay. The efficiency was dependent on the delivery system, superficial load and light dose. Cationic NE-ClAlPc and free-ClAlPc caused photokilling of the both strains of S. aureus. For biofilms, cationic NE-ClAlPc reduced cell metabolism by 80 and 73% of susceptible and resistant strains, respectively. Although anionic NE-ClAlPc caused a significant CFU/ml reduction for MSSA and MRSA, it was not capable of reducing MRSA biofilm metabolism. This therapy may represent an alternative treatment for eradicating resistant strains.


Assuntos
Anti-Infecciosos/farmacologia , Biofilmes/efeitos dos fármacos , Indóis/química , Compostos Organometálicos/química , Fotoquimioterapia/métodos , Sistemas de Liberação de Medicamentos , Emulsões , Humanos , Luz , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Suspensões
19.
J Am Dent Assoc ; 145(11): 1120-8, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25359643

RESUMO

BACKGROUND: Hemostatic agents have been used clinically in dentistry for many years to control bleeding. The authors reviewed scientific publications in which researchers investigated the effects of hemostatic agents on dentin and enamel surfaces and on bonding of adhesive systems and resin cements. TYPES OF STUDIES REVIEWED: The authors screened PubMed and Scopus databases for studies in English published from 1980 to 2013. They read the titles and abstracts to identify literature that fulfilled the inclusion criteria. The authors included studies in which researchers evaluated the hemostatic action on the dentin and enamel surfaces or its influence on the bond strength of adhesive systems or resin cements. They used cross-referencing to identify more articles. RESULTS: Twenty in vitro studies met the inclusion criteria. Investigators in 12 of these studies evaluated the bond strength to contaminated dentin. Investigators in 10 of these studies reported a significant decrease in bond strength. Those in two studies evaluated the influence of a hemostatic agent on the dental enamel and reported decreases in bond strength. Researchers also reported significant increases in microleakage of self-etching adhesives on contaminated dentin. Scanning electron microscopy revealed partial removal of the smear layer or an etching effect of dentin as a result of the application of hemostatic agents on dentin. PRACTICAL IMPLICATIONS: Adhesive procedures may be affected adversely when performed on dentin and enamel contaminated by hemostatic agents. Hemostatic agents may induce changes in the dentin surface morphology. The results of this review indicate that the bond strength of self-etching adhesive systems is affected more negatively than is that of etch-and-rinse systems. The authors found that a 60-second application of ethylenediaminetetraacetic acid followed by a water spray restored the bond strength of a self-etching adhesive to dentin; use of phosphoric acid for 15 seconds followed by a water spray also was an effective cleaning method. Direct comparison of selected studies was not possible, however, mainly because of methodological differences hampering definitive conclusions.


Assuntos
Colagem Dentária , Cimentos Dentários/química , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Hemostáticos/química , Ataque Ácido Dentário , Resinas Compostas/química , Análise do Estresse Dentário , Adesivos Dentinários/química , Microscopia Eletrônica de Varredura , Propriedades de Superfície
20.
Biomed Res Int ; 2014: 895049, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24587995

RESUMO

The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (H2O2) on dental pulp cells. The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mM α-T for 60 minutes. They were then exposed or not to H2O2 for 30 minutes. In positive and negative control groups, the cells were exposed to culture medium with or without H2O2 (0.018%), respectively. Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests; α = 5%). Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control. Cells pretreated with α-T at 1, 3, 5, and 10 mM concentrations and exposed to H2O2 had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively. These values were significantly lower than those observed in the positive control, thereby showing a protective effect of α-T against the H2O2 toxicity. Overall, the vitamin E α-T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H2O2. The most effective cell protection was provided by 5 and 10 mM concentrations of α-T.


Assuntos
Sobrevivência Celular/efeitos dos fármacos , Vitamina E/administração & dosagem , alfa-Tocoferol/administração & dosagem , Meios de Cultura , Polpa Dentária/citologia , Polpa Dentária/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/toxicidade , Vitamina E/química , alfa-Tocoferol/química
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