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Int J Sport Nutr Exerc Metab ; 27(1): 18-24, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27616716


The purpose of this investigation was to quantify the effects of storage temperature, duration, and the urinary sediment on urinary hydration markers. Thirty-six human urine samples were analyzed fresh and then the remaining sample was separated into 24 separate vials, six in each of the following four temperatures: 22 °C, 7 °C, -20 °C, and -80 °C. Two of each sample stored in any given temperature, were analyzed after 1, 2, and 7 days either following vortexing or centrifugation. Each urine sample was analyzed for osmolality (UOsm), urine specific gravity (USG), and urine color (UC). UOsm was stable at 22 °C, for 1 day (+5-9 mmol∙kg-1, p > .05) and at 7 °C, UOsm up to 7 days (+8-8 mmol∙kg-1, p > .05). At -20 and -80 °C, UOsm decreased after 1, 2, and 7 days (9-61 mmol∙kg-1, p < .05). Vortexing the sample before analysis further decreased only UOsm in the -20 °C and -80 °C storage. USG remained stable up to 7 days when samples were stored in 22 °C or 7 °C (p > .05) but declined significantly when stored in -20 °C, and -80 °C (p < .001). UC was not stable in any of the storing conditions for 1, 2, and 7 days. In conclusion, these data indicate that urine specimens analyzed for UOsm or USG remained stable in refrigerated (7 °C) environment for up to 7 days, and in room temperature for 1 day. However, freezing (-20 and -80 °C) samples significantly decreased the values of hydration markers.

Biomarcadores/urina , Desidratação/urina , Manejo de Espécimes , Urinálise , Adulto , Peso Corporal , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Concentração Osmolar , Análise de Regressão , Gravidade Específica , Temperatura , Fatores de Tempo
J Strength Cond Res ; 30(3): 621-5, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26907836


Swimming, either competitively or leisurely, is a unique activity that involves prolonged exercise while immersed in stable water temperatures. This environment could have an influence on the hydration status of swimmers independently of fluid balance. Forty-six healthy adolescent swimmers (26 males and 20 females; 12.8 ± 2.3 years; 50.6 ± 13.4 kg) were studied during a typical training session in an indoor swimming pool. First morning, prepractice and postpractice urine samples were tested for osmolality and specific gravity, whereas all athletes consumed fluids ad libitum. Sixty-seven percent of the athletes were hypohydrated (urine osmolality [Uosm] ≥700 mmol·kg(-1)) based on their first morning urine sample, which increased to 78% immediately before training. During the 2-hour swimming practice, the minimal sweat loss (0.39 ± 0.27 L) combined with ad libitum fluid availability resulted in unchanged body weight (0.1 ± 0.3 kg). Additionally, thirst was similar (before practice: 46 ± 26, after practice: 55 ± 33 mm on a 100-mm visual analog scale) at pretraining and posttraining time points (p > 0.05). Interestingly, postpractice Uosm was reduced significantly compared with the prepractice value (630 vs. 828 mmol·kg(-1); p = 0.001), without any significant change in body weight (0.1 ± 0.3 kg; p > 0.05). In conclusion, the present data indicated that more than two-thirds of the young swimmers appeared in their practice suboptimally hydrated. Although no changes in body mass were observed during the swimming practice, the decrease in urine hydration markers after swimming might less accurately reflect hydration state.

Desidratação/urina , Condicionamento Físico Humano/fisiologia , Natação/fisiologia , Equilíbrio Hidroeletrolítico , Adolescente , Peso Corporal , Criança , Ingestão de Líquidos , Feminino , Humanos , Masculino , Concentração Osmolar , Gravidade Específica , Sudorese , Sede