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1.
Mar Pollut Bull ; 157: 111357, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32658706

RESUMO

In this study, Bacillus cereus was cultivated in a mineral medium composed of 2% frying oil and 0.12% peptone to produce a biosurfactant. The production was scaled up from flasks to 1.2-, 3.0- and 50-L bioreactors, where surface tension achieved 28.7, 27.5 and 32 mN/m and biosurfactant concentration 4.3, 4.6 and 4.7 g/L, respectively. The biosurfactant was characterized as anionic, while nuclear magnetic resonance, thin-layer chromatography and gas chromatography analyses revealed its lipopeptide nature. Toxicity tests showed survival rates of the fish Poecilia vivipara and the bivalve Anomalocardia brasiliana higher than 90% and 55%, respectively, thus suggesting the use of this biosurfactant in marine environment depollution. Moreover, the biosurfactant stimulated the growth of autochthonous microorganisms independently of the presence of motor oil in bioassays performed in seawater. These results demonstrate that the biosurfactant is biocompatible and has potential for industrial-scale production and application to bioremediation of oil spills-polluted marine environment.


Assuntos
Poluição por Petróleo , Petróleo , Bacillus cereus , Biodegradação Ambiental , Tensoativos
2.
Braz. j. infect. dis ; 22(5): 438-441, Sept.-Oct. 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1039218

RESUMO

ABSTRACT Acinetobacter baumannii is one of the most frequent Gram-negative opportunistic pathogens associated with hospital-acquired infection worldwide. We briefly describe A. baumannii isolates that were recovered from surrounding ICU bed surfaces, exhibiting multidrug resistance phenotype and belonging to some widely spread clonal complexes of clinical A. baumannii isolates.

3.
Langmuir ; 34(37): 11121-11125, 2018 09 18.
Artigo em Inglês | MEDLINE | ID: mdl-30169040

RESUMO

The work presents a full physicochemical characterization of sulfonated cellulose beads prepared from Cladophora nanocellulose intended for use in biological systems. 2,3-Dialdehyde cellulose (DAC) beads were sulfonated, and transformation of up to 50% of the aldehyde groups was achieved, resulting in highly charged and porous materials compared to the compact surface of the DAC beads. The porosity could be tailored by adjusting the degree of sulfonation, and a subsequent reduction of the aldehyde groups to hydroxyl groups maintained the bead structure without considerable alteration of the surface properties. The thermal stability of the DAC beads was significantly increased with the sulfonation and reduction reactions. Raman spectroscopy also showed to be a useful technique for the characterization of sulfonated cellulose materials.

4.
Braz J Infect Dis ; 22(5): 438-441, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30265856

RESUMO

Acinetobacter baumannii is one of the most frequent Gram-negative opportunistic pathogens associated with hospital-acquired infection worldwide. We briefly describe A. baumannii isolates that were recovered from surrounding ICU bed surfaces, exhibiting multidrug resistance phenotype and belonging to some widely spread clonal complexes of clinical A. baumannii isolates.


Assuntos
Acinetobacter baumannii/isolamento & purificação , Leitos/microbiologia , Farmacorresistência Bacteriana Múltipla , Unidades de Terapia Intensiva , Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/genética , Bactérias/efeitos dos fármacos , Bactérias/isolamento & purificação , Brasil , Infecção Hospitalar/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Genes Bacterianos , Testes de Sensibilidade Microbiana , Centros de Atenção Terciária
5.
Brain Res ; 1687: 60-65, 2018 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-29496478

RESUMO

Chronic constriction injury (CCI) of the sciatic nerve elicits changes in neuropeptide expression on the dorsal root ganglia (DRG). The neural mobilization (NM) technique is a noninvasive method that has been proven clinically effective in reducing pain. The aim of this study was to analyze the expression of substance P, transient receptor potential vanilloid 1 (TRPV1) and opioid receptors in the DRG of rats with chronic constriction injury and to compare it to animals that received NM treatment. CCI was performed on adult male rats. Each animal was submitted to 10 sessions of neural mobilization every other day, starting 14 days after the CCI injury. At the end of the sessions, the DRG (L4-L6) were analyzed using Western blot assays for substance P, TRPV1 and opioid receptors (µ-opioid receptor, δ-opioid receptor and κ-opioid receptor). We observed a decreased substance P and TRPV1 expression (48% and 35%, respectively) and an important increase of µ-opioid receptor expression (200%) in the DRG after NM treatment compared to control animals. The data provide evidence that NM promotes substantial changes in neuropeptide expression in the DRG; these results may provide new options for treating neuropathic pain.


Assuntos
Regulação da Expressão Gênica/fisiologia , Manipulações Musculoesqueléticas/métodos , Neuralgia/reabilitação , Neuralgia/terapia , Neurônios/metabolismo , Neuropeptídeos/metabolismo , Animais , Modelos Animais de Doenças , Gânglios Espinais/patologia , Masculino , Força Muscular/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Neuralgia/patologia , Neuropeptídeos/genética , Ratos , Ratos Wistar , Receptores Opioides/metabolismo , Substância P/metabolismo , Canais de Cátion TRPV/metabolismo
6.
Molecules ; 23(3)2018 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-29518966

RESUMO

Sulfonated cellulose beads were prepared by oxidation of Cladophora nanocellulose to 2,3-dialdehyde cellulose followed by sulfonation using bisulfite. The physicochemical properties of the sulfonated beads, i.e., high surface area, high degree of oxidation, spherical shape, and the possibility of tailoring the porosity, make them interesting candidates for the development of immunosorbent platforms, including their application in extracorporeal blood treatments. A desired property for materials used in such applications is blood compatibility; therefore in the present work, we investigate the hemocompatibility of the sulfonated cellulose beads using an in vitro whole blood model. Complement system activation (C3a and sC5b-9 levels), coagulation activation (thrombin-antithrombin (TAT) levels) and hemolysis were evaluated after whole blood contact with the sulfonated beads and the results were compared with the values obtained with the unmodified Cladophora nanocellulose. Results showed that neither of the cellulosic materials presented hemolytic activity. A marked decrease in TAT levels was observed after blood contact with the sulfonated beads, compared with Cladophora nanocellulose. However, the chemical modification did not promote an improvement in Cladophora nanocellulose hemocompatibility in terms of complement system activation. Even though the sulfonated beads presented a significant reduction in pro-coagulant activity compared with the unmodified material, further modification strategies need to be investigated to control the complement activation by the cellulosic materials.


Assuntos
Materiais Biocompatíveis/química , Celulose/química , Clorófitas/química , Nanopartículas/química , Fenômenos Químicos , Nanopartículas/ultraestrutura , Tamanho da Partícula , Porosidade
7.
Sci Rep ; 7(1): 8670, 2017 08 17.
Artigo em Inglês | MEDLINE | ID: mdl-28819324

RESUMO

The efficient conversion of light energy into chemical energy is key for sustainable human development. Several photocatalytic systems based on photovoltaic electrolysis have been used to produce hydrogen via water reduction. However, in such devices, light harvesting and proton reduction are carried separately, showing quantum efficiency of about 10-12%. Here, we report a nano-hybrid photocatalytic assembly that enables concomitant reductive hydrogen production and pollutant oxidation with solar-to-fuel efficiencies up to 20%. The modular architecture of this plasmonic material allows the fine-tuning of its photocatalytic properties by simple manipulation of a reduced number of basic components.

9.
Lasers Med Sci ; 32(3): 495-501, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28138810

RESUMO

For better evaluation of the efficacy of low-level laser therapy in treating painful diabetic neuropathy and in protecting nerve fiber damage, we conducted a study with type 1 diabetic rats induced by streptozotocin. It is well known that diabetic peripheral neuropathy is the leading cause of pain in those individuals who suffer from diabetes. Despite the efficacy of insulin in controlling glucose level in blood, there is no effective treatment to prevent or reverse neuropathic damage for total pain relief.Male Wistar rats were divided into saline, vehicle, and treatment groups. A single intraperitoneal (i.p.) injection of streptozotocin (STZ) (85 mg/kg) was administered for the induction of diabetes. The von Frey filaments were used to assess nociceptive thresholds (allodynia). Behavioral measurements were accessed 14, 28, 48, and 56 days after STZ administration. Rats were irradiated with GaAs Laser (Gallium Arsenide, Laserpulse, Ibramed Brazil) emitting a wavelength of 904 nm, an output power of 45 mWpk, beam spot size at target 0.13 cm2, a frequency of 9500 Hz, a pulse time 60 ns, and an energy density of 6,23 J/cm2.The application of four sessions of low-level laser therapy was sufficient to reverse allodynia and protect peripheral nerve damage in diabetic rats.The results of this study indicate that low-level laser therapy is feasible to treat painful diabetic condition in rats using this protocol. Although its efficacy in reversing painful stimuli and protecting nerve fibers from damage was demonstrated, this treatment protocol must be further evaluated in biochemical levels to confirm its biological effects.


Assuntos
Diabetes Mellitus Tipo 1/complicações , Neuropatias Diabéticas/radioterapia , Hiperalgesia/radioterapia , Lasers Semicondutores/uso terapêutico , Terapia com Luz de Baixa Intensidade/métodos , Animais , Diabetes Mellitus Experimental/fisiopatologia , Lasers Semicondutores/efeitos adversos , Terapia com Luz de Baixa Intensidade/efeitos adversos , Masculino , Dor/complicações , Ratos , Ratos Wistar , Estreptozocina
10.
Biomacromolecules ; 17(3): 1224-33, 2016 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-26886265

RESUMO

This work presents an insight into the relationship between cell response and physicochemical properties of Cladophora cellulose (CC) by investigating the effect of CC functional group density on the response of model cell lines. CC was carboxylated by electrochemical TEMPO-mediated oxidation. By varying the amount of charge passed through the electrolysis setup, CC materials with different degrees of oxidation were obtained. The effect of carboxyl group density on the material's physicochemical properties was investigated together with the response of human dermal fibroblasts (hDF) and human osteoblastic cells (Saos-2) to the carboxylated CC films. The introduction of carboxyl groups resulted in CC films with decreased specific surface area and smaller total pore volume compared with the unmodified CC (u-CC). While u-CC films presented a porous network of randomly oriented fibers, a compact and aligned fiber pattern was depicted for the carboxylated-CC films. The decrease in surface area and total pore volume, and the orientation and aggregation of the fibers tended to augment parallel to the increase in the carboxyl group density. hDF and Saos-2 cells presented poor cell adhesion and spreading on u-CC, which gradually increased for the carboxylated CC as the degree of oxidation increased. It was found that a threshold value in carboxyl group density needs be reached to obtain a carboxylated-CC film with cytocompatibility comparable to commercial tissue culture material. Hence, this study demonstrates that a normally bioinert nanomaterial can be rendered bioactive by carefully tuning the density of charged groups on the material surface, a finding that not only may contribute to the fundamental understanding of biointerface phenomena, but also to the development of bioinert/bioactive materials.


Assuntos
Adesão Celular , Celulose Oxidada/química , Nanofibras/química , Linhagem Celular , Celulose Oxidada/farmacologia , Fibroblastos/efeitos dos fármacos , Humanos , Membranas Artificiais , Osteoblastos/efeitos dos fármacos
11.
Rev Bras Ortop ; 50(3): 266-9, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26229928

RESUMO

OBJECTIVE: To evaluate the reproducibility of the radiographic classifications of Gartland and the Association for Osteosynthesis/Association for the Study of Internal Fixation (AO/ASIF) for supracondylar fractures of the humerus in children. METHODS: On two occasions, 50 radiographs in anteroposterior and lateral views were evaluated by three pediatric orthopedists in accordance with the Gartland and AO/ASIF pediatric classifications. Their responses were subjected to statistical analysis consisting of calculation of the κ coefficient to assess the intra- and interobserver concordance, in both classifications. RESULTS: The strength of the intraobserver concordance was high or near perfect for the three examiners in the two classification systems. The strength of the interobserver concordance was high in the two systems, with κ coefficients of 0.756 for the Gartland classification and 0.766 for the AO/ASIF classification. CONCLUSION: The Gartland and AO/ASIF classification systems showed similar reproducibility and performance. High strength of concordance was seen in the intra- and interobserver analyses.

12.
Acta paul. enferm ; 28(5): 433-439, jul.-ago. 2015. tab, graf
Artigo em Português | LILACS, BDENF - Enfermagem | ID: lil-766135

RESUMO

Objetivo: Avaliar a resistência microbiana a medicamentos de bactérias isoladas de equipamentos próximos aos pacientes da Unidade de Terapia Intensiva. Métodos Trata-se de um estudo transversal. As amostras foram coletadas com swabs umedecidos em Trypticase Soy Broth, semeados posteriormente em Ágar Sangue de Carneiro e MacConkey. A identificação fenotípica ocorreu com base na morfologia das cepas e resultados bioquímicos. A análise da resistência aos medicamentos foi baseada no método de disco-difusão de Kirby-Bauer. Resultados Apresentaram-se contaminados 94,4% dos equipamentos analisados. Os microrganismos isolados mais frequentes foram: Acinetobacter sp., Staphylococcus aureus e Pseudomonas sp. Cerca de 75% de Acinetobacter sp. apresentaram resistência à piperacilina associada a tazobactam, meropenem e levofloxacina. Da mesma forma, 36,3% de S. aureus apresentaram-se resistentes à oxacilina e 10% dos isolados de Pseudomonas sp. foram resistentes aos medicamentos testados. Conclusão A maioria dos isolados apresentaram elevadas taxas de resistência microbiana aos medicamentos.


Objective: Evaluate drug resistance of bacteria isolated from equipment placed close to patients in an Intensive Care Unit of a hospital in Caruaru/Pernambuco, Brazil. Methods This is a cross-sectional study. The samples were collected with swabs moistened with Trypticase Soy Broth, which were then cultured in sheep blood agar and MacConkey agar. The phenotypic identification performed was based on the morphology of the strains and biochemical results. The drugs resistance analysis was based on Kirby-Bauer’s Disk Diffusion protocol. Results A rate of 94.4% of the analyzed equipment was contaminated. The most frequently isolated microorganisms were: Acinetobacter sp., Staphylococcus aureus and Pseudomonas sp. Just about 75% of Acinetobacter sp. was resistant to piperacillin associated to tazobactam, meropenem and levofloxacin. Similarly, 36.3% of S. aureus showed resistance to oxacillin and 10% of Pseudomonas sp. was resistant to the drugs tested. Conclusion Most of the microorganisms presented high levels of resistance to the drugs.


Assuntos
Resistência Microbiana a Medicamentos , Contaminação de Equipamentos , Unidades de Terapia Intensiva , Infecção Hospitalar/prevenção & controle , Estudos Transversais , Epidemiologia Descritiva
13.
Rev. bras. ortop ; 50(3): 266-269, May-Jun/2015. tab
Artigo em Inglês | LILACS | ID: lil-753151

RESUMO

OBJETIVO:Avaliar a reprodutibilidade das classificações radiográficas de Gartland e Association for Osteosynthesis/Association for the Study of Internal Fixation (AO/ASIF) para fraturas supracondilianas de úmero em crianças.MÉTODOS:Em duas ocasiões foram avaliadas por três cirurgiões ortopedistas pediátricos 50 radiografias nas incidências anteroposteriores e perfil de acordo com as classificações de Gartland e AO/ASIF pediátrica. As respostas foram submetidas à análise estatística pelo cálculo do coeficiente κ para avaliar a concordância intra- e interobservador, em ambas as classificações.RESULTADOS:A força de concordância intraobservador foi grande ou quase perfeita para os três examinadores nos dois sistemas de classificação. A força de concordância interobservador foi grande nos dois sistemas, com coeficiente κ de 0,756 para classificação de Gartland e de 0,766 para classificação AO/ASIF.CONCLUSÃO:Os sistemas de classificação de Gartland e AO/ASIF mostraram reprodutibilidade e desempenho similar. Observou-se grande força de concordância nas análises intra- e interobservador.


OBJECTIVE: To evaluate the reproducibility of the radiographic classifications of Gartland and the Association for Osteosynthesis/Association for the Study of Internal Fixation (AO/ASIF) for supracondylar fractures of the humerus in children. METHODS: On two occasions, 50 radiographs in anteroposterior and lateral views were evaluated by three pediatric orthopedists in accordance with the Gartland and AO/ASIF pediatric classifications. Their responses were subjected to statistical analysis consisting of calculation of the κ coefficient to assess the intra- and interobserver concordance, in both classifications. RESULTS: The strength of the intraobserver concordance was high or near perfect for the three examiners in the two classification systems. The strength of the interobserver concordance was high in the two systems, with κ coefficients of 0.756 for the Gartland classification and 0.766 for the AO/ASIF classification. CONCLUSION: The Gartland and AO/ASIF classification systems showed similar reproducibility and performance. High strength of concordance was seen in the intra- and interobserver analyses.


Assuntos
Humanos , Masculino , Feminino , Criança , Fraturas do Úmero/classificação , Fraturas do Úmero , Variações Dependentes do Observador , Reprodutibilidade dos Testes
14.
ABCD arq. bras. cir. dig ; 27(4): 275-279, Nov-Dec/2014. tab
Artigo em Inglês | LILACS | ID: lil-735683

RESUMO

BACKGROUND: Pancreatic cancer has a high mortality rate due to late diagnosis and aggressive behavior. The prognosis is poor, with 5-year survival occurring in less than 5% of cases. AIM: To analyze demographic characteristics, comorbidities, type of procedure and early postoperative complications of patients with pancreatic cancer submitted to surgical treatment. METHODS: Cross-sectional study with analysis of 28 medical records of patients with malignant tumors of the pancreas in a 62 month. Data collection was performed from the medical records of the hospital. RESULTS: Of the total, 53,6% were male and the mean age was 60.25 years. According to the procedure, 53,6% was submitted to duodenopancreactectomy the remainder to biliodigestive derivation or distal pancreatectomy. The ductal adenocarcinoma occurred in 82,1% and 92,9% of tumors were located in the pancreatic head. Early postoperative complications occurred in 64,3% of cases and the most prevalent was intra-abdominal abscess (32,1%). Among duodenopancreactectomies 77,8% had early postoperative complications. CONCLUSION: Its necessary to encourage early detection of tumors of the pancreas to raise the number operations with curative intent. Refinements in surgical techniques and surgical teams can diminish postoperative complications and, so, operative morbimortality can also decrease over time. .


RACIONAL: O câncer de pâncreas apresenta alta taxa de mortalidade por conta do diagnóstico tardio e comportamento agressivo. O prognóstico é reservado com sobrevida de cinco anos em menos de 5% dos casos. OBJETIVO: Analisar as características demográficas, as comorbidades, o tipo de procedimento e as intercorrências pós-operatórias precoces dos pacientes de câncer de pâncreas submetidos ao tratamento cirúrgico. MÉTODOS: Estudo transversal, que analisou 28 prontuários de pacientes portadores de tumores malignos do pâncreas, no período de 62 meses. A coleta de dados foi realizada a partir dos prontuários médicos do hospital. RESULTADOS: Do total de participantes 53,6% eram do sexo masculino. A média de idade foi de 60,25 anos. Em relação ao procedimento, 53,6% foram submetidos à duodenopancreactectomia e o restante à derivação biliodigestiva ou pancreatectomia corpo-caudal. O adenocarcinoma ductal ocorreu em 82,1% e 92,9% dos tumores estavam localizados na cabeça do pâncreas. As complicações pós-operatórias precoces ocorreram em 64,3%, e a mais prevalente foi abscesso intra-abdominal (32,1%). Entre as duodenopancreatectomias, 77,8% apresentaram complicações pós-operatórias precoces. CONCLUSÃO: Há necessidade de se incentivar a detecção precoce dos tumores de pâncreas para que se consiga realizar mais operações com intenção curativa. Também, é necessário o aprimoramento das técnicas operatórias e das equipes cirúrgicas para que as complicações pós-operatórias e a morbimortalidade operatória diminuam ao longo do tempo. .


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pancreatectomia , Neoplasias Pancreáticas/cirurgia , Complicações Pós-Operatórias/epidemiologia , Estudos Transversais , Neoplasias Pancreáticas/complicações
15.
Arq Bras Cir Dig ; 27(4): 275-9, 2014.
Artigo em Inglês, Português | MEDLINE | ID: mdl-25626938

RESUMO

BACKGROUND: Pancreatic cancer has a high mortality rate due to late diagnosis and aggressive behavior. The prognosis is poor, with 5-year survival occurring in less than 5% of cases. AIM: To analyze demographic characteristics, comorbidities, type of procedure and early postoperative complications of patients with pancreatic cancer submitted to surgical treatment. METHODS: Cross-sectional study with analysis of 28 medical records of patients with malignant tumors of the pancreas in a 62 month. Data collection was performed from the medical records of the hospital. RESULTS: Of the total, 53,6% were male and the mean age was 60.25 years. According to the procedure, 53,6% was submitted to duodenopancreactectomy the remainder to biliodigestive derivation or distal pancreatectomy. The ductal adenocarcinoma occurred in 82,1% and 92,9% of tumors were located in the pancreatic head. Early postoperative complications occurred in 64,3% of cases and the most prevalent was intra-abdominal abscess (32,1%). Among duodenopancreactectomies 77,8% had early postoperative complications. CONCLUSION: Its necessary to encourage early detection of tumors of the pancreas to raise the number operations with curative intent. Refinements in surgical techniques and surgical teams can diminish postoperative complications and, so, operative morbimortality can also decrease over time.


Assuntos
Pancreatectomia , Neoplasias Pancreáticas/cirurgia , Complicações Pós-Operatórias/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Pancreáticas/complicações
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