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1.
Langmuir ; 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31565937

RESUMO

The synthesis of organic-solvent-dispersible gold nanoparticles in reverse micelles of didodecyldimethylammonium bromide (DDAB) is revisited in the present investigation. Some parameters of synthesis, specifically the reaction volume and the concentration of the reducing agent, were slightly modified obtaining directly monodisperse gold nanocrystals (AuNCs) without the need to use additional active surfactants or additional treatments such as digestive ripening. Interestingly, most of the obtained AuNCs display the same exposed crystalline faces composed of six bounding facets (four {111} faces and two {002} faces), corresponding to single-crystalline face-centered cubic (fcc) nanoparticles with a cuboctahedron shape. When these AuNCs are subsequently functionalized with 1-decanethiol (C10H21SH) or 1-dodecanethiol (C12H25SH) they don´t experience significant changes in their size or crystalline texture, however, they self-aggregate directly in the suspension at room temperature into faceted supramolecular structures, and exhibit collective plasmonic excitations. Such self-organization is reversible under heating treatments allowing the observation of the influence of the AuNCs aggregation state on their plasmonic properties. Fast Fourier infrared spectroscopy reveals that thiols only replace partially the DDAB molecules, thus, DDAB molecules remain present in the thiol-capped AuNCs. In order to turn the thiol-capped nanocrystals into water-dispersible nanocrystals and extend their technological potential, they are stabilized with poloxamer 407 obtaining highly stable purple colloids in water.

2.
Reumatol Clin ; 2019 Aug 06.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31399351

RESUMO

BACKGROUND/OBJECTIVES: Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by swelling, tenderness and destruction of synovial joints, leading to severe disability and premature mortality. The aim of the study was to determine the diagnostic accuracy of the 3 isotypes of rheumatoid factor (RF), anti-citrullinated peptide antibodies (ACPA) and the combination of both, for the diagnosis of rheumatoid arthritis (RA) in non-selected patients with inflammatory arthralgia. METHODS: We include 129 patients with inflammatory Arthalgia from a third level reference Center of rheumatic diseases in Monterrey, México. Their samples were analyzed for RF isotypes (IgA, IgG, and IgM) by ELISA (EUROINMUN), using a cut-off of 20IU/ml, and for ACPA's 5IU/ml; a medical examination was performed to obtain the definitive diagnoses of the patients. Data analysis was carried out using ROC curves for the measurement of sensitivity, specificity, for diagnostic accuracy to verify if the use of 3 RF isotypes and ACPA had a better prediction for the diagnosis of RA than use only one isotype and the ACPA alone. RESULTS: The ROC showed a sensitivity and specificity of the different antibodies with different cut-off points, being the best for the IgM with 0.802 followed by ACPA, IgA and IgG with 0.771, 0.63, and 0.728 respectively without statistical difference, the sensitivity and specificity of the combination of the 4 antibodies were 81.4 and 73.66%. CONCLUSION: In non-selected patients with inflammatory arthralgia, the combination of ACPA and isotypes of RF did not demonstrate more sensibility and specificity than IgM isoform of rheumatoid factor measurement only. We recommend that in the clinical scenario of arthralgia, where the diagnoses are Lupus, Sjogren syndrome and Osteoarthritis, RF IgM isoform is used followed by ACPA.

3.
Dev Med Child Neurol ; 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31410843

RESUMO

The aim of the study was to redefine the phenotype of Allan-Herndon-Dudley syndrome (AHDS), which is caused by mutations in the SLC16A2 gene that encodes the brain transporter of thyroid hormones. Clinical phenotypes, brain imaging, thyroid hormone profiles, and genetic data were compared to the existing literature. Twenty-four males aged 11 months to 29 years had a mutation in SLC16A2, including 12 novel mutations and five previously described mutations. Sixteen patients presented with profound developmental delay, three had severe intellectual disability with poor language and walking with an aid, four had moderate intellectual disability with language and walking abilities, and one had mild intellectual disability with hypotonia. Overall, eight had learned to walk, all had hypotonia, 17 had spasticity, 18 had dystonia, 12 had choreoathetosis, 19 had hypomyelination, and 10 had brain atrophy. Kyphoscoliosis (n=12), seizures (n=7), and pneumopathies (n=5) were the most severe complications. This study extends the phenotypic spectrum of AHDS to a mild intellectual disability with hypotonia. Developmental delay, hypotonia, hypomyelination, and thyroid hormone profile help to diagnose patients. Clinical course depends on initial severity, with stable acquisition after infancy; this may be adversely affected by neuro-orthopaedic, pulmonary, and epileptic complications. WHAT THIS PAPER ADDS: Mild intellectual disability is associated with SLC16A2 mutations. A thyroid hormone profile with a free T3 /T4 ratio higher than 0.75 can help diagnose patients. Patients with SLC16A2 mutations present a broad spectrum of neurological phenotypes that are also observed in other hypomyelinating disorders. Axial hypotonia is a consistent feature of Allan-Herndon-Dudley syndrome and leads to specific complications.

4.
Genes (Basel) ; 10(9)2019 08 22.
Artigo em Inglês | MEDLINE | ID: mdl-31443423

RESUMO

Neurofibromatosis type 1 (NF1) is an autosomal dominant disease with complete penetrance but high variable expressivity. NF1 is caused by loss-of-function mutations in the NF1 gene, a negative regulator of the RAS-MAPK pathway. The NF1 gene has one of the highest mutation rates in human disorders, which may explain the outbreak of independent de novo variants in the same family. Here, we report the co-occurrence of pathogenic variants in the NF1 and SPRED1 genes in six families with NF1 and Legius syndrome, using next-generation sequencing. In five of these families, we observed the co-occurrence of two independent NF1 variants. All NF1 variants were classified as pathogenic, according to the American College of Medical Genetics and Genomics and the Association for Molecular Pathology (ACMG-AMP) guidelines. In the sixth family, one sibling inherited a complete deletion of the NF1 gene from her mother and carried a variant of unknown significance in the SPRED1 gene. This variant was also present in her brother, who was diagnosed with Legius syndrome, a differential diagnosis of NF1. This work illustrates the complexity of molecular diagnosis in a not-so-rare genetic disease.

5.
Hacia promoc. salud ; 24(1): 97-111, ene.-jun. 2019. tab
Artigo em Espanhol | LILACS-Express | ID: biblio-1002031

RESUMO

Resumen Objetivo: Determinar la capacidad predictiva de la felicidad sobre el funcionamiento familiar del adulto mayor en tres ciudades de Colombia. Materiales y métodos: Estudio analítico, transversal, de fuente primaria, con 1514 encuestas aplicadas a personas de 60 años en adelante, en 2016, residentes en las ciudades de Medellín, Barranquilla y Pasto. Se indagó por la funcionalidad familiar a través del APGAR familiar; aunado a las características demográficas, sociales y del estado de salud físico, mental y funcional de la persona mayor. Se realizó análisis univariado, bivariado y multivariado con el fin de ajustar por las variables confusoras y se calculó un modelo predictivo con el método backward (Wald). Resultados: Las características asociadas al funcionamiento familiar fueron: ciudad de residencia; sexo femenino; tener pareja sentimental; alto nivel educativo; no padecer trastornos mentales; percepción de felicidad, sin riesgo de depresión; baja vulnerabilidad y contar con apoyo social. Las que aportan a su predicción fueron ser mujer, ser feliz, no tener depresión, no ser vulnerable, tener apoyo instrumental y social adecuado y no haber sido maltratado. La felicidad es el mayor predictor, según el modelo calculado, con sensibilidad del 92,6 % y especificidad del 40,8 %. Conclusión: Si se fortalece el bienestar subjetivo de la persona mayor se permitirá ver la vejez con una imagen positiva como facilitador de buenas relaciones familiares e integrante activo de la sociedad.


Abstract Objective: To determine the predictive capacity of happiness on the family functioning of the elderly in three cities of Colombia. Materials and methods: Analytical, cross sectional, primary source study with 1514 surveys applied to people aged 60 and older, residents in the cities of Medellín, Barranquilla and Pasto in 2016. Family functionality was explored with the family APGAR scale, along with demographic, social and the physical, mental, functional and health status of the elderly. Univariate, bivariate and multivariate analyzes were carried out to adjust the confounding variables and a predictive model was calculated with the backward method (Wald). Results: The characteristics associated to family functioning were: city of residence, female gender, having a sentimental partner, high educational level, not suffering from mental disorders, perception of happiness without risk of depression, low vulnerability, and having social support. The characteristics that contributed to this prediction were: being a woman, being happy, not having depression, not being vulnerable, having adequate instrumental and social support, and not having been mistreated. Happiness is the greatest predictor according to the calculated model with sensitivity of 92.6% and specificity of 40.8%. Conclusion: if the subjective well-being of the elder is strengthened, it will be possible to see old age with a positive image as a facilitator of good family relationships and an active member of society.


Resumo Objetivo: Determinar a capacidade preditiva da felicidade sobre o funcionamento familiar do adulto maior em três cidades de Colômbia. Materiais e métodos: Estudo analítico, transversal, de fonte primaria, com 1514 enquetes aplicadas a pessoas de 60 anos em adiante, em 2016, residentes nas cidades de Medellín, Barranquilla e Pasto. Pesquisou-se pela funcionalidade familiar a través do APGAR familiar; aunado às caraterísticas demográficas, sociais e do estado de saúde físico, mental e funcional dos idosos. Realizou-se analise uni variado, bivariado e multivariado com o fim de ajustar as variáveis confessoras e se calculou um modelo indutivo com o método backward (Wald). Resultados: As características associadas ao funcionamento familiar foram: cidade de residência; sexo feminino; ter casal sentimental; alto nível educativo; não padecer transtornos mentais; percepção da felicidade, sem risco de depressão; baixa vulnerabilidade e contar com apoio social. As que ajudam a sua predição foram ser mulher, ser feliz, não ter depressão, não ser vulnerável, ter apoio instrumental e social adequado e não ter sido maltratado. A felicidade é o maior indutor, segundo o modelo calculado, com sensibilidade do 92,6 % e especificidade do 40,8 %. Conclusão: Sim se fortalece o bem estar subjetivo do idoso se permitirá ver a velhice com uma imagem positiva como facilitador de boas relações familiares e integrante ativo da sociedade.

6.
Enferm. clín. (Ed. impr.) ; 29(3): 178-185, mayo-jun. 2019. graf, tab
Artigo em Espanhol | LILACS-Express | ID: ibc-ET2-2008

RESUMO

Objetivos: Evaluar si la aplicación de una terapia de relajación reduce las cifras tensionales en pacientes hipertensos y si existe mejora en varios parámetros que pueden ejercer influencia sobre la presión arterial tales como ansiedad, calidad de vida y sueño. Métodos: Estudio cuasiexperimental (medidas antes-después). Participaron 25 pacientes de Atención Primaria con hipertensión arterial mal controlada con fármacos. La intervención consistió en terapia de relajación constituida por 3 técnicas: relajación pasiva de Schwartz-Haynes, respiración diafragmática y visualización imaginaria. Se realizaron un total de 14 sesiones grupales (2/semana) de 30 min. Se analizaron los valores de presión arterial sistólica y diastólica al inicio y final del programa de relajación implantado y después de cada una de las sesiones del programa. Para la medición de los parámetros se emplearon los siguientes cuestionarios: Calidad del Sueño de Pittsburgh, Calidad de Vida en Hipertensión Arterial, Ansiedad Estado-Rasgo. Resultados: Tras la intervención, observamos una reducción de la presión arterial sistólica de 20mmHg (p<0,001) y de la diastólica de 8mmHg (p<0,001). En cuanto a los otros parámetros analizados, la calidad del sueño mejoró significativamente (p<0,001), así como la calidad de vida (p<0,001) y la ansiedad estado (p=0,004). Conclusiones: La terapia de relajación tuvo efectos positivos en los parámetros de presión arterial, así como en los demás factores evaluados. En nuestra opinión, estrategias de este tipo deben ser estudiadas de forma más exhaustiva para considerar su inclusión en Atención Primaria


Objectives: To evaluate whether the application of a relaxation therapy reduces the blood pressure in hypertensive patients and whether there is improvement in several parameters which can influence blood pressure such as anxiety, quality of life and sleep. Methods: A quasi-experimental study (measures before-after) was performed in 25 Primary Care patients with hypertension poorly controlled by pharmacological treatment. The intervention consisted of relaxation therapy composed of 3techniques: passive relaxation of Schwartz-Haynes, diaphragmatic breathing and imaginary visualization. A total of 14 group sessions of 30min each (2/week) were conducted. Systolic and diastolic blood pressure were taken at the beginning and end of the relaxation programme implemented and after each of the programme sessions. The Pittsburgh Sleep Quality, Quality of Life Hypertension, State-Trait Anxiety and Perceived Stress questionnaires were used to measure psychosocial parameters. Results: After intervention, a reduction in systolic blood pressure of 20mmHg (p<.001) and of 8mmHg (p<.001) in diastolic blood pressure was observed. Regarding other factors, sleep quality (p<.001), quality of life (p<.001) and state anxiety (p=.004) were significantly improved. Conclusions: Relaxation therapy had positive effects in improving blood pressure parameters, as well as the other factors evaluated. In our opinion, such strategies should be evaluated more thoroughly to consider their inclusion in Primary Care

8.
Phys Chem Chem Phys ; 21(21): 11214-11225, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31099372

RESUMO

This work reports the experimental study of the ozonolysis of indene in the presence of SO2 and the reaction conditions leading to the formation of secondary aerosols. The reactions have been carried out in a Teflon chamber filled with synthetic air mixtures at atmospheric pressure and room temperature. As in the case of styrene, SO2 plays a key role in the oxidation of the Criegee intermediates and enhances the formation of particulate matter. Thus, for the ozonolysis of indene, nucleation was observed for reacted indene concentrations above (4.5 ± 0.8) × 1011 molecule cm-3 in the absence of SO2 while new particle formation was observed for concentrations one order of magnitude lower, (3 ± 1) × 1010 molecule cm-3, in the presence of SO2. Within the detection limit of the system, SO2 concentrations remained constant during the experiments. The formation of secondary aerosols in the smog chamber was inhibited by H2O and so the potential formation of secondary aerosols under atmospheric conditions depends on the concentration of SO2 and relative humidity. Computational calculations have been performed for the ozonolysis of both indene and styrene in the presence of SO2 and water to identify the reaction channels and species responsible for new particle formation. The release of SO3 and its subsequent conversion into H2SO4 from the reaction of the Criegee intermediate H2COO in the ozonolysis of styrene makes this aromatic have a high potential of aerosol formation in the atmosphere. On the other hand, quantitative conversion of SO2 into SO3 does not occur following the ozonolysis of indene.

9.
Medicine (Baltimore) ; 98(19): e15620, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31083258

RESUMO

RATIONALE: Diffusion tensor imaging (DTI), diffusion tensor tractography (DTT), as well as resting-state-functional magnetic resonance imaging (rsfMRI) are promising methods for assessing patients with disorders of consciousness (DOCs). PATIENT CONCERNS: This work describes the main findings using DTI, DTT, and rsfMRI in a patient with a DOC secondary to an anoxic encephalopathy who had a fatal outcome. She was an 85-year-old woman who presented a cardiac arrest and underwent cardiopulmonary resuscitation for 20 minutes then returning to spontaneous circulation. After sedation withdrawal, 2 days after the event, she remained with a Glasgow Coma Scale score of 3/15 and with an absence of brainstem reflexes. DIAGNOSES: DOC secondary to an anoxic encephalopathy after cardiovascular resuscitation. INTERVENTIONS: A complete brain MRI scan was performed 72 hours after the initial event, including DTI, DTT, and rsfMRI. DTT demonstrated disruption of both ventral and dorsal tegmental tracts bilaterally. DTI showed a reduction of fractional anisotropic level in the mesencephalic nuclei. Moreover, changes in the number of fiber tracts were not evidenced in any portions of the ascending reticular activating system (ARAS). Finally, an increase in the anticorrelated and correlated association among the nuclei in the ARAS and the cortex was evidenced. OUTCOMES: Patient deceased. LESSONS: Neuroimaging demonstrated low FA values in the ARAS, destruction of dorsal and ventral tegmental tracts, as well as hyper-connective (highly correlated or anti-correlated) association among ARAS and cortical nuclei compared with 3 healthy control subjects.


Assuntos
Encéfalo/diagnóstico por imagem , Encéfalo/fisiopatologia , Transtornos da Consciência/diagnóstico por imagem , Transtornos da Consciência/fisiopatologia , Parada Cardíaca/complicações , Imagem por Ressonância Magnética , Idoso de 80 Anos ou mais , Transtornos da Consciência/etiologia , Evolução Fatal , Feminino , Neuroimagem Funcional , Parada Cardíaca/diagnóstico por imagem , Parada Cardíaca/fisiopatologia , Humanos , Imagem por Ressonância Magnética/métodos , Vias Neurais/diagnóstico por imagem , Vias Neurais/fisiopatologia
10.
Chemosphere ; 231: 276-286, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31129409

RESUMO

The formation of secondary organic aerosol (SOA) generated by irradiating styrene in the presence and/or absence of OH, NOx, H2O vapour and seed aerosol has been investigated for the first time. Experiments were conducted in a smog chamber at 298 K and atmospheric pressure. Styrene decay was measured by gas chromatography with a mass spectrometric detector (GC-MS), and the temporal evolution of the aerosol was monitored using a fast mobility particle sizer (FMPS). The SOA yield increases as the initial styrene concentration increases, leading to yields ranging from 1.8% to 3.5% for styrene photolysis, and from 2.4% to 5.0% for its photooxidation. In both cases, the organic aerosol formation can be expressed by a one-product gas/particle partitioning absorption model. The particle number concentration, mass and yield decrease in the presence of NOx and seed aerosol but increase at higher relative humidity (RH). The gas phase and SOA composition were analysed offline using a filter/denuder sampling system simultaneously collecting gas- and particle-phase products. Benzaldehyde was confirmed as the main gas-phase product of the reaction. However, although products in the particle phase were detected, they could not be identified. Moreover, the aqueous filter extracts were analysed using UV-Visible spectrophotometry to determine differences in the optical properties of SOA produced in the presence and absence of NOx. The results from this work may be used to discuss the implications of atmospheric SOA generation from styrene degradation.


Assuntos
Aerossóis/química , Radical Hidroxila/química , Estireno/química , Gases , Modelos Químicos , Processos Fotoquímicos , Fotólise , Água
11.
Artigo em Inglês | MEDLINE | ID: mdl-31129870

RESUMO

High-fat diet (HFD) consumption induces obesity and increases blood glucose, insulin resistance, and metabolic disorders. Recent studies suggest that probiotics might be a novel approach to counteract these effects in the treatment of obesity. Here, we evaluated the effect of Leuconostoc mesenteroides subsp. mesenteroides SD23 on obesity-related metabolic dysfunction. In the present study, mice were randomly divided into four dietary groups: standard diet (C), HFD (OB), standard diet with L. mesenteroides SD23 (CP), and HFD with L. mesenteroides SD23 (OBP). Diets were maintained for 14 weeks. Animal weight was monitored and biochemical and histological analyses were performed after intervention. OB showed metabolic dysfunction, and increased the number of larger adipocytes compared to C. OB induced liver tumor necrosis factor-α (TNF-α) expression, increased cholesterol, leptin, and glucose levels compared to C. OBP reduced body weight, glucose, cholesterol, and leptin levels and improved glucose tolerance compared to OB. OBP also reduced liver steatosis, the number of larger adipocytes in adipose tissue, and reduced the villus height in the small intestine. OBP decreased expression of TNF-α and increased expression of IL-10 in liver. The parameters evaluated in the CP were similar to the C. This study provides novel evidence that dietary intervention with L. mesenteroides SD23 improves metabolic dysfunction related to obesity in HFD-fed mice.

12.
Int J Mol Med ; 43(4): 1559-1574, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30816503

RESUMO

Genome editing reemerged in 2012 with the development of CRISPR/Cas9 technology, which is a genetic manipulation tool derived from the defense system of certain bacteria against viruses and plasmids. This method is easy to apply and has been used in a wide variety of experimental models, including cell lines, laboratory animals, plants, and even in human clinical trials. The CRISPR/Cas9 system consists of directing the Cas9 nuclease to create a site­directed double­strand DNA break using a small RNA molecule as a guide. A process that allows a permanent modification of the genomic target sequence can repair the damage caused to DNA. In the present study, the basic principles of the CRISPR/Cas9 system are reviewed, as well as the strategies and modifications of the enzyme Cas9 to eliminate the off­target cuts, and the different applications of CRISPR/Cas9 as a system for visualization and gene expression activation or suppression. In addition, the review emphasizes on the potential application of this system in the treatment of different diseases, such as pulmonary, gastrointestinal, hematologic, immune system, viral, autoimmune and inflammatory diseases, and cancer.


Assuntos
Proteína 9 Associada à CRISPR/metabolismo , Sistemas CRISPR-Cas/genética , Edição de Genes , Humanos , Modelos Genéticos
14.
J Clin Invest ; 129(3): 1240-1256, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30620337

RESUMO

Sphingolipid imbalance is the culprit in a variety of neurological diseases, some affecting the myelin sheath. We have used whole-exome sequencing in patients with undetermined leukoencephalopathies to uncover the endoplasmic reticulum lipid desaturase DEGS1 as the causative gene in 19 patients from 13 unrelated families. Shared features among the cases include severe motor arrest, early nystagmus, dystonia, spasticity, and profound failure to thrive. MRI showed hypomyelination, thinning of the corpus callosum, and progressive thalamic and cerebellar atrophy, suggesting a critical role of DEGS1 in myelin development and maintenance. This enzyme converts dihydroceramide (DhCer) into ceramide (Cer) in the final step of the de novo biosynthesis pathway. We detected a marked increase of the substrate DhCer and DhCer/Cer ratios in patients' fibroblasts and muscle. Further, we used a knockdown approach for disease modeling in Danio rerio, followed by a preclinical test with the first-line treatment for multiple sclerosis, fingolimod (FTY720, Gilenya). The enzymatic inhibition of Cer synthase by fingolimod, 1 step prior to DEGS1 in the pathway, reduced the critical DhCer/Cer imbalance and the severe locomotor disability, increasing the number of myelinating oligodendrocytes in a zebrafish model. These proof-of-concept results pave the way to clinical translation.

15.
Rev. biol. trop ; 66(4): 1649-1663, oct.-dic. 2018. tab
Artigo em Inglês | LILACS-Express | ID: biblio-1003353

RESUMO

Abstract One way of reducing the input of pollutants into the marine environment is to enforce the use of non-toxic antifouling paints in marine protected areas. Thus, the purpose of this study was to detect marine microorganisms that secrete inhibitory substances against bacteria and microalgae to avoid biofouling on manmade structures in La Paz bay, B.C.S., Mexico. The inhibitory potential of 125 bacteria was evaluated against biofilm-forming bacteria. Crude extracts were obtained with methanol and ethyl acetate from 16 bacterial strains that exhibited antagonistic and antibacterial activity in a preliminary screening. Antibacterial and antimicroalgal assays were performed using crude extracts, the minimum inhibitory concentration (MIC) was determined. The highest activity against bacteria and microalgae was found in two strains, Shewanella algae and Staphylococcus sp. The results of this study suggest that extracts of bacteria from the Gulf of California with antimicrobial properties against biofilm-forming bacteria can also prevent the adhesion of microalgae, which may control the development of biofilm formation and, as a consequence, biofouling.


Resumen Una alternativa para reducir la contaminación en el medio marino es el uso de pinturas anti-incrustantes no tóxicas en áreas marinas protegidas. En el presente estudio se propuso encontrar microorganismos marinos que secreten sustancias capaces de inhibir la adhesión de bacterias y microalgas, de esta manera evitar la bioincrustación en estructuras marinas en la bahía de La Paz, B.C.S., México. Un total de 125 bacterias fueron evaluadas por su capacidad para inhibir el desarrollo de bacterias formadoras de biopelículas. En una selección preliminar de actividad antagónica y antibacteriana, 16 cepas bacterianas mostraron potencial actividad inhibitoria, de las que se obtuvieron los extractos crudos con metanol y acetato de etilo. Se realizaron ensayos antibacterianos y anti-microalgales utilizando los extractos crudos, se determinó la concentración mínima inhibitoria (MIC). Dos cepas mostraron la mayor actividad contra bacterias y microalgas: Shewanella algae y Staphylococcus sp. Los resultados de este estudio sugieren que los extractos de bacterias aisladas en el Golfo de California que poseen propiedades antimicrobianas contra las bacterias formadoras de biofilm y también pueden prevenir la adhesión de microalgas, con lo que se podría controlar el desarrollo de la formación de biopelículas y como consecuencia, el biofouling.

17.
Nat Commun ; 9(1): 4619, 2018 11 05.
Artigo em Inglês | MEDLINE | ID: mdl-30397230

RESUMO

Chromatin remodeling is of crucial importance during brain development. Pathogenic alterations of several chromatin remodeling ATPases have been implicated in neurodevelopmental disorders. We describe an index case with a de novo missense mutation in CHD3, identified during whole genome sequencing of a cohort of children with rare speech disorders. To gain a comprehensive view of features associated with disruption of this gene, we use a genotype-driven approach, collecting and characterizing 35 individuals with de novo CHD3 mutations and overlapping phenotypes. Most mutations cluster within the ATPase/helicase domain of the encoded protein. Modeling their impact on the three-dimensional structure demonstrates disturbance of critical binding and interaction motifs. Experimental assays with six of the identified mutations show that a subset directly affects ATPase activity, and all but one yield alterations in chromatin remodeling. We implicate de novo CHD3 mutations in a syndrome characterized by intellectual disability, macrocephaly, and impaired speech and language.

18.
Hacia promoc. salud ; 24(1): [97]-[111], 13-noviembre-2018.
Artigo em Inglês, Espanhol, Português | LILACS-Express | ID: biblio-968767

RESUMO

Objetivo: Determinar la capacidad predictiva de la felicidad sobre el funcionamiento familiar del adulto mayor en tres ciudades de Colombia. Materiales y métodos: Estudio analítico, transversal, de fuente primaria, con 1514 encuestas aplicadas a personas de 60 años en adelante, en 2016, residentes en las ciudades de Medellín, Barranquilla y Pasto. Se indagó por la funcionalidad familiar a través del APGAR familiar; aunado a las características demográficas, sociales y del estado de salud físico, mental y funcional de la persona mayor. Se realizó análisis univariado, bivariado y multivariado con el fin de ajustar por las variables confusoras y se calculó un modelo predictivo con el método backward (Wald). Resultados: Las características asociadas al funcionamiento familiar fueron: ciudad de residencia; sexo femenino; tener pareja sentimental; alto nivel educativo; no padecer trastornos mentales; percepción de felicidad, sin riesgo de depresión; baja vulnerabilidad y contar con apoyo social. Las que aportan a su predicción fueron ser mujer, ser feliz, no tener depresión, no ser vulnerable, tener apoyo instrumental y social adecuado y no haber sido maltratado. La felicidad es el mayor predictor, según el modelo calculado, con sensibilidad del 92,6 % y especificidad del 40,8 %. Conclusión: Si se fortalece el bienestar subjetivo de la persona mayor se permitirá ver la vejez con una imagen positiva como facilitador de buenas relaciones familiares e integrante activo de la sociedad.


Objective: To determine the predictive capacity of happiness on the family functioning of the elderly in three cities of Colombia. Materials and methods: Analytical, cross sectional, primary source study with 1514 surveys applied to people aged 60 and older, residents in the cities of Medellín, Barranquilla and Pasto in 2016. Family functionality was explored with the family APGAR scale, along with demographic, social and the physical, mental, functional and health status of the elderly. Univariate, bivariate and multivariate analyzes were carried out to adjust the confounding variables and a predictive model was calculated with the backward method (Wald). Results: The characteristics associated to family functioning were: city of residence, female gender, having a sentimental partner, high educational level, not suffering from mental disorders, perception of happiness without risk of depression, low vulnerability, and having social support. The characteristics that contributed to this prediction were: being a woman, being happy, not having depression, not being vulnerable, having adequate instrumental and social support, and not having been mistreated. Happiness is the greatest predictor according to the calculated model with sensitivity of 92.6% and specificity of 40.8%. Conclusion: if the subjective well-being of the elder is strengthened, it will be possible to see old age with a positive image as a facilitator of good family relationships and an active member of society.


Objetivo: Determinar a capacidade preditiva da felicidade sobre o funcionamento familiar do adulto maior em três cidades de Colômbia. Materiais e métodos: Estudo analítico, transversal, de fonte primaria, com 1514 enquetes aplicadas a pessoas de 60 anos em adiante, em 2016, residentes nas cidades de Medellín, Barranquilla e Pasto. Pesquisou-se pela funcionalidade familiar a través do APGAR familiar; aunado às caraterísticas demográficas, sociais e do estado de saúde físico, mental e funcional dos idosos. Realizou-se analise uni variado, bivariado e multivariado com o fim de ajustar as variáveis confessoras e se calculou um modelo indutivo com o método backward (Wald). Resultados: As características associadas ao funcionamento familiar foram: cidade de residência; sexo feminino; ter casal sentimental; alto nível educativo; não padecer transtornos mentais; percepção da felicidade, sem risco de depressão; baixa vulnerabilidade e contar com apoio social. As que ajudam a sua predição foram ser mulher, ser feliz, não ter depressão, não ser vulnerável, ter apoio instrumental e social adequado e não ter sido maltratado. A felicidade é o maior indutor, segundo o modelo calculado, com sensibilidade do 92,6 % e especificidade do 40,8 %. Conclusão: Sim se fortalece o bem estar subjetivo do idoso se permitirá ver a velhice com uma imagem positiva como facilitador de boas relações familiares e integrante ativo da sociedade

19.
Int. j. psychol. psychol. ther. (Ed. impr.) ; 18(3): 301-313, oct. 2018. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-174407

RESUMO

No disponible


The aim of these paper was to evaluate the effect of three types of instructions (general, precise, and specific) on the performance of a problem solving task and its relation with verbal regulation using the Tower of London (TOL) as methodological tool. Participants (30 adults and 30 children) held a session of 24 trials of different complexity, carried out according to the number of movements required to reach the goal (4, 5 and 6). The results showed significant differences between children and adults in all dependent variables, acording on the number of movements required in trials. Although less strongly, differences were also observed as a result of the type of instruction to which the participants were exposed. The analysis of verbal reports served as indicators of the type of verbal regulation that mediated the performance. It is discussed the need to elaborate comparative studies that allow to systematically evaluate the types of verbal regulation deployed during problem solving tasks in participants of different ages


Assuntos
Humanos , Criança , Adulto , Comportamento Verbal , Pesquisa Comportamental/métodos , Escala de Avaliação Comportamental/estatística & dados numéricos , Resolução de Problemas , Comportamento Social , Tomada de Decisões , Modelos Educacionais , Controle Social Formal , Análise e Desempenho de Tarefas
20.
Enferm Clin ; 2018 Aug 09.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-30100138

RESUMO

OBJECTIVES: To evaluate whether the application of a relaxation therapy reduces the blood pressure in hypertensive patients and whether there is improvement in several parameters which can influence blood pressure such as anxiety, quality of life and sleep. METHODS: A quasi-experimental study (measures before-after) was performed in 25 Primary Care patients with hypertension poorly controlled by pharmacological treatment. The intervention consisted of relaxation therapy composed of 3techniques: passive relaxation of Schwartz-Haynes, diaphragmatic breathing and imaginary visualization. A total of 14 group sessions of 30min each (2/week) were conducted. Systolic and diastolic blood pressure were taken at the beginning and end of the relaxation programme implemented and after each of the programme sessions. The Pittsburgh Sleep Quality, Quality of Life Hypertension, State-Trait Anxiety and Perceived Stress questionnaires were used to measure psychosocial parameters. RESULTS: After intervention, a reduction in systolic blood pressure of 20mmHg (p<.001) and of 8mmHg (p<.001) in diastolic blood pressure was observed. Regarding other factors, sleep quality (p<.001), quality of life (p<.001) and state anxiety (p=.004) were significantly improved. CONCLUSIONS: Relaxation therapy had positive effects in improving blood pressure parameters, as well as the other factors evaluated. In our opinion, such strategies should be evaluated more thoroughly to consider their inclusion in Primary Care.

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