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1.
J Cancer Res Ther ; 17(2): 547-550, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34121706

RESUMO

Purpose: Health emergency due to COVID-19 started in Uruguay on March 13, 2020; our mastology unit tried to ensure adequate oncological care, and protect patients from the virus infection and complications. Objective: To assess the health care activities in the "peak" of the pandemic during 3 months. Materials and Methods: we collected data from the electronic health record. Results: There were a total of 293 medical appointments from 131 patients (221 face-to-face), that decreased by 16.7% compared to the same period in 2019 (352 appointments). The medical appointments were scheduled to evaluate the continuity of systemic treatment or modifications (95 patients; 72.5%), follow-up (17; 12.9%), first-time consultation (12; 9.1%), and assess paraclinical studies (7; 5.3%). The patients were on hormone therapy (81 patients; 74%), chemotherapy (CT) (21; 19%), and anti-HER2 therapies (9; 8%). New twenty treatments were initiated. Of the 14 patients that were on adjuvant/neoadjuvant CT, 9 (64.3%) continued with the same regimen with the addition of prophylactic granulocyte-colony-stimulating factors (G-CSF), and 5 (35.7%), who were receiving weekly paclitaxel, continued the treatment with no changes. Of the seven patients that were on palliative CT, 2 (28.5%) continued the treatment with the addition of G-CSF, 3 (42.8%) continued with weekly capecitabine or paclitaxel with no treatment changes, and 2 (28.5%) changed their treatment regimen (a less myelosuppressive regimen was selected for one and due to progression of the disease in the other patient). The ninety patients who were receiving adjuvant, neoadjuvant, or palliative criteria hormone therapy and/or anti-HER2 therapies, continued the treatment with no changes. Conclusions: The evidence suggests that, although medical appointments decreased by approximately 17%, we could maintain healthcare activities, continued most of the treatments while the most modified was CT with G-CSF to avoid myelosuppression.


Assuntos
Neoplasias da Mama/tratamento farmacológico , COVID-19/epidemiologia , Continuidade da Assistência ao Paciente/estatística & dados numéricos , Atenção à Saúde/estatística & dados numéricos , Oncologia/estatística & dados numéricos , Adulto , Idoso , Idoso de 80 Anos ou mais , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Medula Óssea/efeitos dos fármacos , Neoplasias da Mama/complicações , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/imunologia , COVID-19/imunologia , COVID-19/prevenção & controle , COVID-19/transmissão , Controle de Doenças Transmissíveis/normas , Continuidade da Assistência ao Paciente/organização & administração , Atenção à Saúde/organização & administração , Atenção à Saúde/normas , Registros Eletrônicos de Saúde/estatística & dados numéricos , Feminino , Fator Estimulador de Colônias de Granulócitos/administração & dosagem , Hematopoese/efeitos dos fármacos , Hematopoese/imunologia , Humanos , Oncologia/organização & administração , Oncologia/normas , Pessoa de Meia-Idade , Pandemias/prevenção & controle , Encaminhamento e Consulta/normas , Encaminhamento e Consulta/estatística & dados numéricos , Estudos Retrospectivos , Telemedicina/organização & administração , Telemedicina/normas , Telemedicina/estatística & dados numéricos , Triagem/organização & administração , Triagem/normas , Uruguai/epidemiologia
2.
An Acad Bras Cienc ; 93(2): e20190841, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34190842

RESUMO

To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson's trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.


Assuntos
Anuros , Reprodução , Animais , Feminino , Gametogênese , Masculino , Ovário , Testículo
3.
Anat Rec (Hoboken) ; 304(7): 1420-1438, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33099895

RESUMO

The objective of this study was to characterize the external morphology of Salvator meriane embryos in different stages of embryonic development and establish a relationship with the ultrastructure of the shell from oviductal transit to hatching. A total of 120 embryos were analyzed to describe their external morphology, and 78 eggs were used for the analysis of the shell. For embryonic development, the series was established according to the total length of the body. We established 40 embryonic stages from the primitive streak. In the early stages, the external morphological features are the C-shaped body, the maxillary, and mandibular fusion processes with the frontal process and the fusion of the forelimb with the digital plate. In the middle stages, the eyelid appears, and there are claws on the toes, cornification of fingers, and the onset of pigmentation. The last stage of embryonic development is characterized by the beginning of the formation of the scales, appear the toenails, and finalize the entire pigmentation. Regarding the relationship that exists with the ultrastructure of the egg during development, it was possible to observe a marked change in the composition of the shell and well-marked compaction during embryonic development, which may be related to the transport of calcium during embryonic ossification. Our results allowed us to show the complete sequence of embryonic development, determining the laying stage for this species. It was possible to establish a relationship with the ultrastructure of the eggshell from the oviductal transit to the moment of hatching.

4.
Transcription ; 11(3-4): 117-133, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32748694

RESUMO

Plants have a high level of developmental plasticity that allows them to respond and adapt to changes in the environment. Among the environmental cues, light controls almost every aspect of A. thaliana's life cycle, including seed maturation, seed germination, seedling de-etiolation and flowering time. Light signals induce massive reprogramming of gene expression, producing changes in RNA polymerase II transcription, alternative splicing, and chromatin state. Since splicing reactions occur mainly while transcription takes place, the regulation of RNAPII transcription has repercussions in the splicing outcomes. This cotranscriptional nature allows a functional coupling between transcription and splicing, in which properties of the splicing reactions are affected by the transcriptional process. Chromatin landscapes influence both transcription and splicing. In this review, we highlight, summarize and discuss recent progress in the field to gain a comprehensive insight on the cross-regulation between chromatin state, RNAPII transcription and splicing decisions in plants, with a special focus on light-triggered responses. We also introduce several examples of transcription and splicing factors that could be acting as coupling factors in plants. Unravelling how these connected regulatory networks operate, can help in the design of better crops with higher productivity and tolerance.

5.
Anat Rec (Hoboken) ; 301(9): 1527-1543, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30312031

RESUMO

We presented a comparative study of two species of South American bats, Myotis albescens and Eumops patagonicus, about prenatal development. This study was carried out using 60 specimens, which were measured and photographed, and the embryonic stage was assigned by the staging system for Carollia perspicillata. We observed that the chorionic vesicle showed similarities in the disposition of the extraembryonic membranes, but they differed in characteristics of their yolk sac; in E. patagonicus, it was more glandular than M. albescens. M. albescens presented a well-developed discoid placenta with a caudal antimesometrial position, but E. patagonicus presented a diffuse placenta, which persists until the end of gestation and a discoid placenta in the uterus-tubal junction. In the embryogenesis, early stages, middle stages, and late stages were defined. In the early stage, the embryonic morphology is similar in the two species. The middle stage is characterized by the muzzle and pinna formation, fore and hind limb regionalization, and the formation of the patagium primordium. In the late stage, the overall growth of the embryo occurs. Its fore and hind limbs, patagium, and the typical craniofacial features are configured. We conclude that in early stages of development, the embryonic morphology of M. albescens and E. patagonicus is similar, while in late stages differences are evident; mainly the craniofacial structures and uropatagium configuration characteristics that allow their classification at the family level. Moreover, differences in time of fusion of maxillary and mandibular process were registered. This could be related to the morphology of the muzzle of each species. Anat Rec, 301:1527-1543, 2018. © 2018 Wiley Periodicals, Inc.


Assuntos
Quirópteros/embriologia , Desenvolvimento Embrionário/fisiologia , Membranas Extraembrionárias/embriologia , Animais , Feminino , Placenta/fisiologia , Gravidez
6.
Front Plant Sci ; 9: 1275, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30214454

RESUMO

The megagametophyte of mature seeds of Araucaria angustifolia consists of cells with thin walls, one or more nuclei, a central vacuole storing proteins, and a cytoplasm rich in amyloplasts, mitochondria and lipid bodies. In this study, we describe the process of mobilization of reserves and analyzed the dismantling of the tissue during germination, using a range of well-established markers of programmed cell death (PCD), including: morphological changes in nuclei and amyloplasts, DNA degradation, and changes in nuclease profiles. TUNEL reaction and DNA electrophoresis demonstrate that DNA fragmentation in nuclei occurs at early stages of germination, which correlates with induction of specific nucleases. The results of the present study add knowledge on the dismantling of the megagametophyte of genus Araucaria, a storage tissue that stores starch as the main reserve substance, as well as on the PCD pathway, by revealing new insights into the role of nucleases and the expression patterns of putative nuclease genes during germination.

7.
J Bacteriol ; 185(5): 1634-41, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12591881

RESUMO

A recombinant clone encoding enzymes for Klebsiella pneumoniae O12-antigen lipopolysaccharide (LPS) was found when we screened for serum resistance of a cosmid-based genomic library of K. pneumoniae KT776 (O12:K80) introduced into Escherichia coli DH5alpha. A total of eight open reading frames (ORFs) (wb(O12) gene cluster) were necessary to produce K. pneumoniae O12-antigen LPS in E. coli K-12. A complete analysis of the K. pneumoniae wb(O12) cluster revealed an interesting coincidence with the wb(O4) cluster of Serratia marcescens from ORF5 to ORF8 (or WbbL to WbbA). This prompted us to generate mutants of K. pneumoniae strain KT776 (O12) and to study complementation between the two enterobacterial wb clusters using mutants of S. marcescens N28b (O4) obtained previously. Both wb gene clusters are examples of ABC 2 transporter-dependent pathways for O-antigen heteropolysaccharides. The wzm-wzt genes and the wbbA or wbbB genes were not interchangeable between the two gene clusters despite their high level of similarity. However, introduction of three cognate genes (wzm-wzt-wbbA or wzm-wzt-wbbB) into mutants unable to produce O antigen allowed production of the specific O antigen. The K. pneumoniae O12 WbbL protein performs the same function as WbbL from S. marcescens O4 in either the S. marcescens O4 or E. coli K-12 genetic background.


Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Transportadores de Cassetes de Ligação de ATP/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Klebsiella pneumoniae/metabolismo , Antígenos O/biossíntese , Sequência de Aminoácidos , Clonagem Molecular , Escherichia coli/genética , Teste de Complementação Genética , Klebsiella pneumoniae/genética , Dados de Sequência Molecular , Família Multigênica , Mutação , Análise de Sequência de Proteína , Homologia de Sequência do Ácido Nucleico , Serratia marcescens/genética
8.
Buenos Aires; Nueva Visión; 2a. ed; 1984. 211 p. ^e20cm.(Psicología contemporánea).
Monografia em Espanhol | LILACS-Express | BINACIS | ID: biblio-1196708
9.
Buenos Aires; Tarshis; 1a. ed; 19?. 108 p. ^e22 cm.
Monografia em Espanhol | LILACS-Express | BINACIS | ID: biblio-1198941
10.
Buenos Aires; Nueva Vision; 1a. ed; 1979. 211 p. 20 cm.(Psicología Contemporánea). (73907).
Monografia em Espanhol | BINACIS | ID: bin-73907
11.
Buenos Aires; Tarshis; 1a. ed; 19?. 108 p. 22 cm. (73863).
Monografia em Espanhol | BINACIS | ID: bin-73863
12.
Buenos Aires; Nueva Visión; 2a. ed; 1984. 211 p. 20cm.(Psicología contemporánea). (71220).
Monografia em Espanhol | BINACIS | ID: bin-71220
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