Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 16 de 16
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
J Clin Endocrinol Metab ; 105(3)2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31606738

RESUMO

CONTEXT: Oncostatin M (OSM) plays a key role in inflammation, but its regulation and function during obesity is not fully understood. OBJECTIVE: The aim of this study was to evaluate the relationship of OSM with the inflammatory state that leads to impaired glucose homeostasis in obesity. We also assessed whether OSM immunoneutralization could revert metabolic disturbances caused by a high-fat diet (HFD) in mice. DESIGN: 28 patients with severe obesity were included and stratified into two groups: (1) glucose levels <100 mg/dL and (2) glucose levels >100 mg/dL. White adipose tissue was obtained to examine OSM gene expression. Human adipocytes were used to evaluate the effect of OSM in the inflammatory response, and HFD-fed C57BL/6J mice were injected with anti-OSM antibody to evaluate its effects. RESULTS: OSM expression was elevated in subcutaneous and visceral fat from patients with obesity and hyperglycemia, and correlated with Glut4 mRNA levels, serum insulin, homeostatic model assessment of insulin resistance, and inflammatory markers. OSM inhibited adipogenesis and induced inflammation in human adipocytes. Finally, OSM receptor knockout mice had increased Glut4 mRNA levels in adipose tissue, and OSM immunoneutralization resulted in a reduction of glucose levels and Ccl2 expression in adipose tissue from HFD-fed mice. CONCLUSIONS: OSM contributes to the inflammatory state during obesity and may be involved in the development of insulin resistance.


Assuntos
Glucose/metabolismo , Homeostase , Obesidade/metabolismo , Oncostatina M/fisiologia , Adipócitos/citologia , Adulto , Animais , Feminino , Transportador de Glucose Tipo 4/genética , Humanos , Resistência à Insulina , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Receptores de Oncostatina M/fisiologia
2.
Artif Cells Nanomed Biotechnol ; 48(1): 77-83, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31852325

RESUMO

Type 1 diabetes (T1D) is an autoimmune disease caused by the destruction of insulin-producing cells. Due to the ability of apoptotic cells clearance to induce tolerance, we previously generated liposomes rich in phophatidylserine (PS) -a feature of apoptotic cells- loaded with insulin peptides to mimic apoptotic beta-cells. PS-liposomes arrested autoimmunity in experimental T1D through the induction of tolerance. The aim of this study was to investigate the potential of several peptides from different T1D autoantigens encapsulated in (PS)-liposomes for T1D prevention and to assess its safety. T1D autoantigens (Insulin, C-peptide, GAD65 and IA2) were encapsulated in PS-liposomes. Liposomes were administered to the 'gold-standard' model for the study of autoimmune T1D, the Non-Obese Diabetic mouse, that spontaneously develop the disease. Safety and toxicity of liposomes were also determined. Only PS-liposomes encapsulating insulin peptides decrease T1D incidence in the Non-Obese Diabetic mouse model. Disease prevention correlates with a decrease in the severity of the autoimmune islet destruction driven by leukocytes. PS-liposomes neither showed toxic effect nor secondary complications. Among the here referred autoantigens, insulin peptides are the best candidates to be encapsulated in liposomes, like an artificial apoptotic cell, for the arrest of autoimmunity in T1D in a safe manner.

3.
Front Immunol ; 10: 2811, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31849983

RESUMO

Type 1 diabetes (T1D) is prompted by defective immunological tolerance, an event in which dendritic cells (DCs) are crucial as immune response orchestrators. In fact, they contribute to maintaining tolerance to self-antigens, but they can also prompt an immunogenic response against them, leading to autoimmunity. Countless factors can potentially impact on the proper functionality of the DCs, which range from altered subset distribution, impaired phagocytic function to abnormal gene expression. Moreover, in T1D, metabolic dysregulation could impair DC functions as well. Indeed, since T1D clinical course is likely to be more aggressive in children and adolescents and entails severe dysglycemia, the aim of this study was to analyze circulating DCs subpopulations in pediatric T1D at different stages, as well as to characterize their phagocytosis ability and tolerance induction potential. Thus, pediatric patients newly diagnosed with T1D, with established disease and control subjects were recruited. Firstly, DCs subsets from peripheral blood were found quantitatively altered during the first year of disease, but recovered in the second year of progression. Secondly, to study the tolerogenic functionality of DCs, liposomes with phosphatidylserine (PS) were designed to mimic apoptotic beta cells, which are able to induce tolerance, as previously demonstrated by our group in DCs from adult patients with T1D. In this study, monocyte-derived DCs from pediatric patients with T1D and control subjects were assessed in terms of PS-liposomes capture kinetics, and transcriptional and phenotypic changes. DCs from pediatric patients with T1D were found to phagocyte PS-liposomes more slowly and less efficiently than DCs from control subjects, inversely correlating with disease evolution. Nonetheless, the transcription of PS receptors and immunoregulatory genes, cytokine profile, and membrane expression of immunological markers in DCs was consistent with tolerogenic potential after PS-liposomes phagocytosis. In conclusion, T1D progression in childhood entails altered peripheral blood DCs subsets, as well as impaired DCs phagocytosis, although tolerance induction could still function optimally. Therefore, this study provides useful data for patient follow-up and stratification in immunotherapy clinical trials.

4.
Transl Res ; 210: 8-25, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30953609

RESUMO

Type 1 diabetes (T1D) is a chronic metabolic disease of unknown etiology that results from ß-cell destruction. The onset of the disease, which arises after a long asymptomatic period of autoimmune attack, may be followed by a relapsing and remitting progression, a phenomenon that is most evident during the partial remission phase (PR). This stage lasts for a few months, shows minor requirements of exogenous insulin and could be explained by a recovery of immunological tolerance. This study aims to identify new biomarkers at early stages of pediatric T1D that reflect immunoregulatory changes. To that end, pediatric patients with T1D (n = 52) and age-related control subjects (n = 30) were recruited. Immune response-related molecules and lymphocyte subsets were determined starting at T1D onset and until the second year of progression. Results showed that circulating TGF-ß levels decreased during PR, and that betatrophin concentration was increased in all the considered stages without differing among studied checkpoints. Moreover, an increase of regulatory T, B and NK subsets was found during T1D progression, probably reflecting an attempt to restore self-tolerance. By contrast, a reduction in monocyte levels was observed at the early stages of diabetes. The results reveal significant changes in immunological parameters during the different early stages of T1D in children, which could ultimately serve as potential biomarkers to characterize the progression of T1D.


Assuntos
Diabetes Mellitus Tipo 1/imunologia , Diabetes Mellitus Tipo 1/patologia , Proteínas Semelhantes a Angiopoietina/sangue , Biomarcadores/sangue , Índice de Massa Corporal , Estudos de Casos e Controles , Criança , Diabetes Mellitus Tipo 1/sangue , Progressão da Doença , Feminino , Humanos , Memória Imunológica , Subpopulações de Linfócitos/metabolismo , Masculino , Monócitos/metabolismo , Hormônios Peptídicos/sangue , Projetos Piloto , Indução de Remissão , Fator de Crescimento Transformador beta/sangue
5.
Sci Rep ; 9(1): 1235, 2019 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-30718757

RESUMO

Non-genetic factors are crucial in the pathogenesis of type 1 diabetes (T1D), a disease caused by autoimmunity against insulin-producing ß-cells. Exposure to medications in the prenatal period may influence the immune system maturation, thus altering self-tolerance. Prenatal administration of betamethasone -a synthetic glucocorticoid given to women at risk of preterm delivery- may affect the development of T1D. It has been previously demonstrated that prenatal betamethasone administration protects offspring from T1D development in nonobese diabetic (NOD) mice. The direct effect of betamethasone on the immature and mature immune system of NOD mice and on target ß-cells is analysed in this paper. In vitro, betamethasone decreased lymphocyte viability and induced maturation-resistant dendritic cells, which in turn impaired γδ T cell proliferation and decreased IL-17 production. Prenatal betamethasone exposure caused thymus hypotrophy in newborn mice as well as alterations in immune cells subsets. Furthermore, betamethasone decreased ß-cell growth, reduced C-peptide secretion and altered the expression of genes related to autoimmunity, metabolism and islet mass in T1D target tissue. These results support the protection against T1D in the betamethasone-treated offspring and demonstrate that this drug alters the developing immune system and ß-cells. Understanding how betamethasone generates self-tolerance could have potential clinical relevance in T1D.

6.
Front Immunol ; 9: 253, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29491866

RESUMO

Type 1 diabetes (T1D) is a metabolic disease caused by the autoimmune destruction of insulin-producing ß-cells. With its incidence increasing worldwide, to find a safe approach to permanently cease autoimmunity and allow ß-cell recovery has become vital. Relying on the inherent ability of apoptotic cells to induce immunological tolerance, we demonstrated that liposomes mimicking apoptotic ß-cells arrested autoimmunity to ß-cells and prevented experimental T1D through tolerogenic dendritic cell (DC) generation. These liposomes contained phosphatidylserine (PS)-the main signal of the apoptotic cell membrane-and ß-cell autoantigens. To move toward a clinical application, PS-liposomes with optimum size and composition for phagocytosis were loaded with human insulin peptides and tested on DCs from patients with T1D and control age-related subjects. PS accelerated phagocytosis of liposomes with a dynamic typical of apoptotic cell clearance, preserving DCs viability. After PS-liposomes phagocytosis, the expression pattern of molecules involved in efferocytosis, antigen presentation, immunoregulation, and activation in DCs concurred with a tolerogenic functionality, both in patients and control subjects. Furthermore, DCs exposed to PS-liposomes displayed decreased ability to stimulate autologous T cell proliferation. Moreover, transcriptional changes in DCs from patients with T1D after PS-liposomes phagocytosis pointed to an immunoregulatory prolife. Bioinformatics analysis showed 233 differentially expressed genes. Genes involved in antigen presentation were downregulated, whereas genes pertaining to tolerogenic/anti-inflammatory pathways were mostly upregulated. In conclusion, PS-liposomes phagocytosis mimics efferocytosis and leads to phenotypic and functional changes in human DCs, which are accountable for tolerance induction. The herein reported results reinforce the potential of this novel immunotherapy to re-establish immunological tolerance, opening the door to new therapeutic approaches in the field of autoimmunity.


Assuntos
Apoptose/imunologia , Células Dendríticas/imunologia , Diabetes Mellitus Tipo 1/imunologia , Tolerância Imunológica/imunologia , Fosfatidilserinas/imunologia , Adolescente , Adulto , Autoantígenos/imunologia , Células Cultivadas , Feminino , Humanos , Imunoterapia/métodos , Lipossomos , Masculino , Pessoa de Meia-Idade , Mimetismo Molecular/imunologia , Fagocitose , Adulto Jovem
7.
Nanomedicine (Lond) ; 12(11): 1231-1242, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28593827

RESUMO

AIM: Based on the ability of apoptosis to induce immunological tolerance, liposomes were generated mimicking apoptotic cells, and they arrest autoimmunity in Type 1 diabetes. Our aim was to validate the immunotherapy in other autoimmune disease: multiple sclerosis. MATERIALS & METHODS: Phosphatidylserine-rich liposomes were loaded with disease-specific autoantigen. Therapeutic capability of liposomes was assessed in vitro and in vivo. RESULTS: Liposomes induced a tolerogenic phenotype in dendritic cells, and arrested autoimmunity, thus decreasing the incidence, delaying the onset and reducing the severity of experimental disease, correlating with an increase in a probably regulatory CD25+ FoxP3- CD4+ T-cell subset. CONCLUSION: This is the first work that confirms phosphatidylserine-liposomes as a powerful tool to arrest multiple sclerosis, demonstrating its relevance for clinical application.


Assuntos
Autoantígenos/administração & dosagem , Imunoterapia/métodos , Lipossomos/química , Esclerose Múltipla/terapia , Glicoproteína Mielina-Oligodendrócito/administração & dosagem , Peptídeos/administração & dosagem , Fosfatidilserinas/química , Animais , Autoantígenos/imunologia , Autoantígenos/uso terapêutico , Feminino , Camundongos Endogâmicos C57BL , Esclerose Múltipla/imunologia , Glicoproteína Mielina-Oligodendrócito/imunologia , Glicoproteína Mielina-Oligodendrócito/uso terapêutico , Peptídeos/imunologia , Peptídeos/uso terapêutico , Linfócitos T Reguladores/imunologia
8.
Curr Pharm Des ; 23(18): 2623-2643, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28201972

RESUMO

Cell-based tolerogenic therapy is a promising approach for the treatment of autoimmune diseases and transplant rejection. Regulatory T cells and tolerogenic dendritic cells have been particularly explored in the treatment of various autoimmune disorders in experimental models of disease. Although some of these cells have already been tested in a limited number of clinical trials, there is still a need for preclinical research on tolerogenic cells in animal models of autoimmunity. This review will focus on the relevance of data obtained from studies in experimental animal models for the use of tolerogenic cell-based therapy in humans. Also, perspectives for further improvement of tolerogenic cell preparation towards enhanced suppressive activity and stability of the cells will be discussed.


Assuntos
Artrite Reumatoide/terapia , Terapia Baseada em Transplante de Células e Tecidos/métodos , Diabetes Mellitus Tipo 1/terapia , Modelos Animais de Doenças , Esclerose Múltipla/terapia , Animais , Artrite Reumatoide/imunologia , Autoimunidade/efeitos dos fármacos , Autoimunidade/imunologia , Diabetes Mellitus Tipo 1/imunologia , Humanos , Tolerância Imunológica/efeitos dos fármacos , Tolerância Imunológica/imunologia , Imunossupressores/administração & dosagem , Esclerose Múltipla/imunologia , Compostos Orgânicos/administração & dosagem , Linfócitos T Reguladores/efeitos dos fármacos , Linfócitos T Reguladores/imunologia
9.
Mol Cell Endocrinol ; 426: 101-12, 2016 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-26911933

RESUMO

The transmembrane glycoprotein CD26 or dipeptidyl peptidase IV (DPPIV) is a multifunctional protein. In immune system, CD26 plays a role in T-cell function and is also involved in thymic maturation and emigration patterns. In preclinical studies, treatment with DPPIV inhibitors reduces insulitis and delays or even reverses the new -onset of type 1 diabetes (T1D) in non-obese diabetic (NOD) mice. However, the specific mechanisms involved in these effects remain unknown. The aim of the present study was to investigate how DPPIV inhibition modifies the expression of genes in the thymus of NOD mice by microarray analysis. Changes in the gene expression of ß-cell autoantigens and Aire in thymic epithelial cells (TECs) were also evaluated by using qRT-PCR. A DPPIV inhibitor, MK626, was orally administered in the diet for 4 and 6 weeks starting at 6-8 weeks of age. Thymic glands from treated and control mice were obtained for each study checkpoint. Thymus transcriptome analysis revealed that 58 genes were significantly over-expressed in MK626-treated mice after 6 weeks of treatment. Changes in gene expression in the thymus were confined mainly to the immune system, including innate immunity, chemotaxis, antigen presentation and immunoregulation. Most of the genes are implicated in central tolerance mechanisms through several pathways. No differences were observed in the expression of Aire and ß-cell autoantigens in TECs. In the current study, we demonstrate that treatment with the DPPIV inhibitor MK626 in NOD mice alters the expression of the immune response-related genes in the thymus, especially those related to immunological central tolerance, and may contribute to the prevention of T1D.


Assuntos
Dipeptidil Peptidase 4/metabolismo , Inibidores da Dipeptidil Peptidase IV/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Indinavir/farmacologia , Animais , Apresentação do Antígeno/genética , Feminino , Regulação da Expressão Gênica/imunologia , Redes Reguladoras de Genes , Imunomodulação/genética , Camundongos Endogâmicos NOD , Timo/efeitos dos fármacos , Timo/metabolismo , Transcriptoma
10.
PLoS One ; 10(11): e0142186, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26555789

RESUMO

CD26 is a T cell activation marker consisting in a type II transmembrane glycoprotein with dipeptidyl peptidase IV (DPPIV) activity in its extracellular domain. It has been described that DPPIV inhibition delays the onset of type 1 diabetes and reverses the disease in non-obese diabetic (NOD) mice. The aim of the present study was to assess the effect of MK626, a DPPIV inhibitor, in type 1 diabetes incidence and in T lymphocyte subsets at central and peripheral compartments. Pre-diabetic NOD mice were treated with MK626. Diabetes incidence, insulitis score, and phenotyping of T lymphocytes in the thymus, spleen and pancreatic lymph nodes were determined after 4 and 6 weeks of treatment, as well as alterations in the expression of genes encoding ß-cell autoantigens in the islets. The effect of MK626 was also assessed in two in vitro assays to determine proliferative and immunosuppressive effects. Results show that MK626 treatment reduces type 1 diabetes incidence and after 6 weeks of treatment reduces insulitis. No differences were observed in the percentage of T lymphocyte subsets from central and peripheral compartments between treated and control mice. MK626 increased the expression of CD26 in CD8+ T effector memory (TEM) from spleen and pancreatic lymph nodes and in CD8+ T cells from islet infiltration. CD8+TEM cells showed an increased proliferation rate and cytokine secretion in the presence of MK626. Moreover, the combination of CD8+ TEM cells and MK626 induces an immunosuppressive response. In conclusion, treatment with the DPPIV inhibitor MK626 prevents experimental type 1 diabetes in association to increase expression of CD26 in the CD8+ TEM lymphocyte subset. In vitro assays suggest an immunoregulatory role of CD8+ TEM cells that may be involved in the protection against autoimmunity to ß pancreatic islets associated to DPPIV inhibitor treatment.


Assuntos
Linfócitos T CD8-Positivos/efeitos dos fármacos , Diabetes Mellitus Tipo 1/prevenção & controle , Dipeptidil Peptidase 4/efeitos dos fármacos , Inibidores da Dipeptidil Peptidase IV/farmacologia , Fosfato de Sitagliptina/análogos & derivados , Animais , Autoantígenos/genética , Linfócitos T CD8-Positivos/imunologia , Diabetes Mellitus Tipo 1/imunologia , Ilhotas Pancreáticas/efeitos dos fármacos , Ilhotas Pancreáticas/imunologia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos NOD , Fosfato de Sitagliptina/farmacologia , Fator de Crescimento Transformador beta/sangue
11.
PLoS One ; 10(6): e0127057, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26039878

RESUMO

INTRODUCTION: The development of new therapies to induce self-tolerance has been an important medical health challenge in type 1 diabetes. An ideal immunotherapy should inhibit the autoimmune attack, avoid systemic side effects and allow ß-cell regeneration. Based on the immunomodulatory effects of apoptosis, we hypothesized that apoptotic mimicry can help to restore tolerance lost in autoimmune diabetes. OBJECTIVE: To generate a synthetic antigen-specific immunotherapy based on apoptosis features to specifically reestablish tolerance to ß-cells in type 1 diabetes. METHODS: A central event on the surface of apoptotic cells is the exposure of phosphatidylserine, which provides the main signal for efferocytosis. Therefore, phosphatidylserine-liposomes loaded with insulin peptides were generated to simulate apoptotic cells recognition by antigen presenting cells. The effect of antigen-specific phosphatidylserine-liposomes in the reestablishment of peripheral tolerance was assessed in NOD mice, the spontaneous model of autoimmune diabetes. MHC class II-peptide tetramers were used to analyze the T cell specific response after treatment with phosphatidylserine-liposomes loaded with peptides. RESULTS: We have shown that phosphatidylserine-liposomes loaded with insulin peptides induce tolerogenic dendritic cells and impair autoreactive T cell proliferation. When administered to NOD mice, liposome signal was detected in the pancreas and draining lymph nodes. This immunotherapy arrests the autoimmune aggression, reduces the severity of insulitis and prevents type 1 diabetes by apoptotic mimicry. MHC class II tetramer analysis showed that peptide-loaded phosphatidylserine-liposomes expand antigen-specific CD4+ T cells in vivo. The administration of phosphatidylserine-free liposomes emphasizes the importance of phosphatidylserine in the modulation of antigen-specific CD4+ T cell expansion. CONCLUSIONS: We conclude that this innovative immunotherapy based on the use of liposomes constitutes a promising strategy for autoimmune diseases.


Assuntos
Autoantígenos/imunologia , Autoimunidade , Diabetes Mellitus Tipo 1/tratamento farmacológico , Diabetes Mellitus Tipo 1/imunologia , Fosfatidilserinas/uso terapêutico , Animais , Linfócitos T CD4-Positivos/imunologia , Diferenciação Celular , Citocinas/metabolismo , Células Dendríticas/imunologia , Imunoterapia , Injeções Intraperitoneais , Insulina/uso terapêutico , Lipossomos , Camundongos Endogâmicos NOD , Fenótipo
12.
Apoptosis ; 20(3): 263-72, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25604067

RESUMO

Type 1 diabetes (T1D) is a metabolic disease that results from the autoimmune attack against insulin-producing ß-cells in the pancreatic islets of Langerhans. Currently, there is no treatment to restore endogenous insulin secretion in patients with autoimmune diabetes. In the last years, the development of new therapies to induce long-term tolerance has been an important medical health challenge. Apoptosis is a physiological mechanism that contributes to the maintenance of immune tolerance. Apoptotic cells are a source of autoantigens that induce tolerance after their removal by antigen presenting cells (APCs) through a process called efferocytosis. Efferocytosis will not cause maturation in dendritic cells, one of the most powerful APCs, and this process could induce tolerance rather than autoimmunity. However, failure of this mechanism due to an increase in the rate of ß-cells apoptosis and/or defects in efferocytosis results in activation of APCs, contributing to inflammation and to the loss of tolerance to self. In fact, T1D and other autoimmune diseases are associated to enhanced apoptosis of target cells and defective apoptotic cell clearance. Although further research is needed, the clinical relevance of immunotherapies based on apoptosis could prove to be very important, as it has translational potential in situations that require the reestablishment of immunological tolerance, such as autoimmune diseases. This review summarizes the effects of apoptosis of ß-cells towards autoimmunity or tolerance and its application in the field of emerging immunotherapies.


Assuntos
Apoptose/imunologia , Autoimunidade , Células Dendríticas/imunologia , Diabetes Mellitus Tipo 1/imunologia , Tolerância Imunológica , Células Secretoras de Insulina/imunologia , Animais , Proteínas Reguladoras de Apoptose/genética , Proteínas Reguladoras de Apoptose/imunologia , Autoantígenos/genética , Autoantígenos/imunologia , Citocinas/biossíntese , Citocinas/imunologia , Células Dendríticas/patologia , Diabetes Mellitus Tipo 1/genética , Diabetes Mellitus Tipo 1/patologia , Diabetes Mellitus Tipo 1/terapia , Regulação da Expressão Gênica , Humanos , Imunoterapia , Células Secretoras de Insulina/patologia , Camundongos , Linfócitos T/imunologia , Linfócitos T/patologia
13.
Medisan ; 15(4)abr. 2011.
Artigo em Espanhol | LILACS | ID: lil-616192

RESUMO

Se realizó un estudio descriptivo y transversal para determinar el estado de salud bucal de 98 escolares de 5-6 años de la Escuela Primaria 30 de Noviembre de Santiago de Cuba, en el período comprendido de enero a diciembre del 2007. Tanto el examen físico intrabucal y extrabucal efectuado como la revisión de cada historia clínica de los educandos proporcionaron la información necesaria al respecto, que luego fue procesada con la versión 6.0 del programa Epi Info y resumida mediante índices y porcentajes. Los resultados evidenciaron que la salud bucal deficiente podía ser atribuida a la presencia de periodontitis, que en ambas denticiones (temporal y permanente) predominó el componente de dientes obturados y que los estudiantes debían recibir los beneficios de la periodoncia, la ortodoncia y el tratamiento conservador, en ese orden.


A descriptive and cross-sectional study was carried out with the purpose of determining the oral health in 98 schoolcildren between 5-6 years of age at 30 de Noviembre Primary School of Santiago de Cuba, from January to December 2007. Both, physical intraoral and extraoral examination and the review of each medical record of the pupils provided the necessary information in this respect, which was then processed with the program Epi Info 6.0 and summarized by means of indexes and percentages. Results revealed that poor oral health could be attributed to periodontitis, that in both teethings (deciduous and permanent) the component of filled teeth prevailed and schoolchildren should receive the benefits of the periodontics, orthodontics and conservative treatment in that order.


Assuntos
Humanos , Masculino , Feminino , Criança , Serviços de Saúde da Criança , Ensino Fundamental e Médio , Promoção da Saúde , Saúde Bucal , Estudos Transversais , Epidemiologia Descritiva
14.
Medisan ; 15(4)abr. 2011.
Artigo em Espanhol | CUMED | ID: cum-47492

RESUMO

Se realizó un estudio descriptivo y transversal para determinar el estado de salud bucal de 98 escolares de 5-6 años de la Escuela Primaria 30 de Noviembre de Santiago de Cuba, en el período comprendido de enero a diciembre del 2007. Tanto el examen físico intrabucal y extrabucal efectuado como la revisión de cada historia clínica de los educandos proporcionaron la información necesaria al respecto, que luego fue procesada con la versión 6.0 del programa Epi Info y resumida mediante índices y porcentajes. Los resultados evidenciaron que la salud bucal deficiente podía ser atribuida a la presencia de periodontitis, que en ambas denticiones (temporal y permanente) predominó el componente de dientes obturados y que los estudiantes debían recibir los beneficios de la periodoncia, la ortodoncia y el tratamiento conservador, en ese orden(AU)


A descriptive and cross-sectional study was carried out with the purpose of determining the oral health in 98 schoolcildren between 5-6 years of age at 30 de Noviembre Primary School of Santiago de Cuba, from January to December 2007. Both, physical intraoral and extraoral examination and the review of each medical record of the pupils provided the necessary information in this respect, which was then processed with the program Epi Info 6.0 and summarized by means of indexes and percentages. Results revealed that poor oral health could be attributed to periodontitis, that in both teethings (deciduous and permanent) the component of filled teeth prevailed and schoolchildren should receive the benefits of the periodontics, orthodontics and conservative treatment in that order(AU)


Assuntos
Humanos , Masculino , Feminino , Criança , Saúde Bucal , Promoção da Saúde , Ensino Fundamental e Médio , Serviços de Saúde da Criança , Epidemiologia Descritiva , Estudos Transversais
15.
Medisan ; 14(1)ene.-feb. 2010.
Artigo em Espanhol | LILACS | ID: lil-576457

RESUMO

Se realizó una investigación descriptiva y transversal de 98 niños de primer grado del Seminternado 30 de Noviembre de Santiago de Cuba, atendidos en la propia escuela por odontólogos de la Clínica Estomatológica de Vista Alegre durante el 2007, para determinar su estado de salud bucal, así como los hábitos bucales deformantes presentes. Entre las principales alteraciones del complejo bucomaxilofacial sobresalieron: gingivitis, caries, deformidad dentofacial (vestibuloversión y mordida abierta anterior), así como la lengua protráctil como hábito deformante, asociada a la higiene bucal deficiente. Predominaron la dieta cariogénica y la placa dentobacteriana como factores de riesgo. Se recomendó elaborar y ejecutar un programa de actividades de educación para la salud, cuyas acciones estratégicas sean dirigidas a niños, padres, familiares y educadores.


A descriptive and cross-sectional study was carried out in 98 first grade schoolchildren from 30 de Noviembre Day Boarding School of Santiago de Cuba, who were attended at their own school by odontologists from the Dental Clinics of Vista Alegre during 2007 to determine their oral health state and deforming habits. Among the main disorders of the oral and maxillofacial complex were gingivitis, decay, dentofacial deformity (vestibuloversion and anterior open bite), as well as protractile tongue as a deforming habit associated with poor oral hygiene. Cariogenic diet and dentobacterial plaque predominated as risk factors. It was recommended to develop and implement a program of educational activities for health, strategic actions of which are addressed to children, parents, relatives and educators.


Assuntos
Humanos , Pré-Escolar , Criança , Ensino Fundamental e Médio , Sucção de Dedo , Mordida Aberta/diagnóstico , Mordida Aberta/prevenção & controle , Saúde Bucal , Serviços de Odontologia Escolar , Serviços de Saúde Escolar , Hábitos Linguais , Estudos Transversais , Epidemiologia Descritiva
16.
Medisan ; 14(1)ene.-feb. 2010.
Artigo em Espanhol | CUMED | ID: cum-43100

RESUMO

Se realizó una investigación descriptiva y transversal de 98 niños de primer grado del Seminternado 30 de Noviembre de Santiago de Cuba, atendidos en la propia escuela por odontólogos de la Clínica Estomatológica de Vista Alegre durante el 2007, para determinar su estado de salud bucal, así como los hábitos bucales deformantes presentes. Entre las principales alteraciones del complejo bucomaxilofacial sobresalieron: gingivitis, caries, deformidad dentofacial (vestibuloversión y mordida abierta anterior), así como la lengua protráctil como hábito deformante, asociada a la higiene bucal deficiente. Predominaron la dieta cariogénica y la placa dentobacteriana como factores de riesgo. Se recomendó elaborar y ejecutar un programa de actividades de educación para la salud, cuyas acciones estratégicas sean dirigidas a niños, padres, familiares y educadores(AU)


A descriptive and cross-sectional study was carried out in 98 first grade schoolchildren from 30 de Noviembre Day Boarding School of Santiago de Cuba, who were attended at their own school by odontologists from the Dental Clinics of Vista Alegre during 2007 to determine their oral health state and deforming habits. Among the main disorders of the oral and maxillofacial complex were gingivitis, decay, dentofacial deformity (vestibuloversion and anterior open bite), as well as protractile tongue as a deforming habit associated with poor oral hygiene. Cariogenic diet and dentobacterial plaque predominated as risk factors. It was recommended to develop and implement a program of educational activities for health, strategic actions of which are addressed to children, parents, relatives and educators(AU)


Assuntos
Humanos , Pré-Escolar , Criança , Serviços de Odontologia Escolar , Serviços de Saúde Escolar , Hábitos Linguais , Sucção de Dedo , Mordida Aberta/diagnóstico , Mordida Aberta/prevenção & controle , Saúde Bucal , Ensino Fundamental e Médio , Epidemiologia Descritiva , Estudos Transversais
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA