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1.
Forensic Sci Int Genet ; 2(2): e5-8, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19083801

RESUMO

Allelic frequencies and haplotypic composition of 305 male unrelated individuals from the Caribbean Colombian states of Atlántico, Bolívar, Cesar, Córdoba, Guajira, Magdalena and Sucre, were determined using 16 Y-chromosome STR loci. Two hundred and ninety three (293) haplotypes were identified, of which 283 were unique and the other 10 were found twice or thrice in the Caribbean population tested. Haplotypic diversity surpassed the values obtained in other populations, ranging from 99.66% in the population of Sucre to 99.99% in the population of Córdoba. We also calculated the overall haplotypic diversity (99.97%) and the discrimination power of these haplotypes (96.1%) in these groups. Analysis of molecular variance (AMOVA) for 10 Colombian and Spanish populations (3139 haplotypes) reveals low differentiation between the Colombian populations of mainly European descent and large distance to Afroamerican populations living in Colombia.


Assuntos
Cromossomos Humanos Y , Genética Populacional , Repetições de Microssatélites/genética , Grupo com Ancestrais do Continente Africano/genética , Alelos , Colômbia , DNA/genética , DNA/isolamento & purificação , Impressões Digitais de DNA/métodos , Grupo com Ancestrais do Continente Europeu/genética , Frequência do Gene , Geografia , Haplótipos , Humanos , Masculino , Reação em Cadeia da Polimerase , Controle de Qualidade
2.
Forensic Sci Int ; 137(1): 67-73, 2003 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-14550617

RESUMO

Genotype polymorphism studies at the 13 loci STRs included in the combined DNA index system [CODIS and PCR-based short tandem repeat loci, in: Proceedings of the Second European Symposium on Human Identification, Promega Corporation, Madison, WI, 1998, pp. 73-88; J. Forensic Sci. 46 (2001) 453] (CODIS: D3S1358, HUMvWA31, HUMFGA, D8S1179 D21S11, D18S51, D5S818, D13S317, D7S820, HUMTH01, HUMTPOX, HUMCSF1PO and D16S539) were carried out in a sample of 1429 unrelated Colombian individuals belonging to 25 different departments. As many other countries in Latino-America, Colombia shows an important admixture component, basically integrated by Amerindians, European-descendants and African-descendants. Due to the fact that only partial population analyses have been carried out in the country, the main aim of the present analysis is to establish a database of forensic interest based on the widely used CODIS systems covering the main Colombian regions.


Assuntos
Frequência do Gene , Genética Populacional , Polimorfismo Genético , Sequências de Repetição em Tandem , Colômbia , Impressões Digitais de DNA/métodos , Grupos Étnicos/genética , Genótipo , Humanos , Reação em Cadeia da Polimerase
3.
Mem. Inst. Oswaldo Cruz ; 97(8): 1157-1163, Dec. 15, 2002. ilus, tab
Artigo em Inglês | LILACS | ID: lil-326328

RESUMO

In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74 percent being detected by PCR technique, 58 percent by culture and 44 percent by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases


Assuntos
Humanos , Antígenos de Bactérias , Proteínas de Bactérias , Mycobacterium tuberculosis , Tuberculose , Antígenos de Bactérias , Proteínas de Bactérias , Colômbia , Estudo de Avaliação , Amplificação de Genes , Mycobacterium tuberculosis , Reação em Cadeia da Polimerase , Sensibilidade e Especificidade
4.
Mem Inst Oswaldo Cruz ; 97(8): 1157-63, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12563484

RESUMO

In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74% being detected by PCR technique, 58% by culture and 44% by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases.


Assuntos
Antígenos de Bactérias/análise , Mycobacterium tuberculosis/isolamento & purificação , Tuberculose Pulmonar/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos de Bactérias/genética , Criança , Pré-Escolar , Colômbia , Amplificação de Genes , Humanos , Lactente , Pessoa de Meia-Idade , Mycobacterium tuberculosis/genética , Reação em Cadeia da Polimerase , Estudos Retrospectivos , Sensibilidade e Especificidade , Tuberculose/diagnóstico
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