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1.
Clin Oral Investig ; 2020 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-32728940

RESUMO

OBJECTIVES: To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation. MATERIALS AND METHODS: Thirty-six rats' right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin. RESULTS: Denosumab 60 mg/mL reducted ankylosis (p < 0.0001), replacement resorption (p < 0.0001), and tooth resorption, 60 days after replantation, compared to untreated replanted teeth (p < 0.005). Lower bacterial contamination in root surface in the denosumab treatment groups was found, regardless of the concentration used (p < 0.001). Also, denosumab treatment inhibited the expression of RANKL without modulating OPG. Periostin was observed in periodontal ligament of replanted tooth, although this labelling was absent in the ankylosis areas, in both experimental periods. CONCLUSION: Treatment of the root surface with denosumab at 60 mg/mL of rat teeth before delayed replantation reduced dental root resorption compared with the untreated teeth after 60 days. CLINICAL RELEVANCE: Survival of a replanted tooth has been a challenge in clinical practice. The use of a medication, such as denosumab, to limit dental root resorption represents an important therapeutical approach.

2.
Orthod Craniofac Res ; 23(3): 342-350, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32246880

RESUMO

OBJECTIVE: The role of oestrogen in craniofacial growth still remains unclear. Therefore, the present study aimed to assess the effect of oestrogen deficiency on maxilla and mandible dimensions. SETTING AND SAMPLE POPULATION: The study was conducted at the Department of Pediatric Dentistry at the School of Dentistry of Ribeirão Preto, University of São Paulo, and used forty female Wistar rats. MATERIAL AND METHODS: Ovariectomy (OVX) and placebo surgery (Sham) were performed when animals were twenty-one days old (prepubertal stage). Dimensions of the maxilla and mandible were assessed by craniometric analysis using radiographs, during and after puberty of the animals (45 and 63 days old, respectively). Quantitative real-time PCR and immunohistochemical analyses were performed to determine the expression and localization, respectively, of oestrogen receptor alpha (ERα) and oestrogen receptor beta (ERß) in different growth sites of the evaluated structures at puberty. The differences between the groups for each outcome were evaluated using the t test with an established alpha error of 5%. RESULTS: There were significant differences between the OVX and Sham groups for horizontal and vertical linear measurements in the maxilla and the mandible at both pubertal and post-pubertal stages (P < .05). The ovariectomized rats showed significantly greater measures for all dimensions assessed. No differences in gene expression of ERα and ERß were identified at the different growth sites between the OVX and Sham groups (P > .05). Immunohistochemical analyses revealed the presence of both oestrogen receptors in osteoblasts and chondrocytes in the midpalatal suture and mandibular condyle, respectively, in the OVX and Sham groups. CONCLUSION: Our results suggest that oestrogen deficiency from the prepubertal stage might increase the growth of the maxilla and mandible in female rats.


Assuntos
Maxila , Maturidade Sexual , Animais , Criança , Feminino , Humanos , Mandíbula , Ovariectomia , Ratos , Ratos Wistar
3.
Braz Dent J ; 31(1): 57-62, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32159707

RESUMO

The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.


Assuntos
Fumar Cigarros , Terapia com Luz de Baixa Intensidade , Animais , Masculino , Ratos , Ratos Wistar , Extração Dentária , Cicatrização
4.
J Orthod ; 47(1): 65-71, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32000574

RESUMO

OBJECTIVE: To investigate the association of genetic markers in ESR1 and ESR2 with craniofacial measurements. DESIGN: Cross-sectional study. SETTING: School of Dentistry of Ribeirão Preto, University of São Paulo. PARTICIPANTS: A total of 146 biologically unrelated, self-reported Caucasian Brazilians with no syndromic conditions were included. METHODS: Sagittal and vertical measurements (ANB, S-N, Ptm'-A', Co-Gn, Go-Pg, N-Me, ANS-Me, S-Go and Co-Go) from lateral cephalograms were examined for craniofacial evaluation. DNA was extracted from saliva and genetic markers in ESR1 (rs2234693 and rs9340799) and in ESR2 (rs1256049 and rs4986938) were analysed by real-time polymerase chain reaction. Hardy-Weinberg equilibrium was evaluated using the Chi-square test within each marker. The associations between craniofacial dimensions and genotypes were analysed by linear regression and adjusted by sex and age. The established alpha was 5%. RESULTS: Individuals carrying CC in ESR1 rs2234693 had a decrease of -3.146 mm in ANS-Me (P = 0.044). In addition, rs4986938 in ESR2 was associated with S-N measurement (P = 0.009/ ß = -3.465). This marker was also associated with Go-Pg measurement, in which the CC genotype had a decrease of -3.925 mm in the length of the mandibular body (P = 0.043). CONCLUSION: The present study suggests that in ESR1 and ESR2 are markers for variations in the craniofacial dimensions. However, further research should confirm the results.


Assuntos
Receptor alfa de Estrogênio , Receptor beta de Estrogênio , Estudos Transversais , Marcadores Genéticos , Humanos , Polimorfismo de Nucleotídeo Único
5.
Braz. dent. j ; 31(1): 57-62, Jan.-Feb. 2020. graf
Artigo em Inglês | LILACS-Express | ID: biblio-1089270

RESUMO

Abstract The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.


Resumo O objetivo deste estudo foi investigar os efeitos da terapia a laser de baixo nível (LLLT) e a fumaça de cigarro na sinalização da osteoclastogênese do alvéolo após extração dentária, em ratos. Sessenta ratos Wistar machos foram distribuídos aleatoriamente em quatro grupos com 15 animais cada: Grupo de controle (com extração do molar superior direito (EM), Experimental I (EM e LLLT), Experimental II (EM e fumaça de cigarro) e Grupo Experimental III (EM, LLLT e fumaça de cigarro). A eutanásia foi realizada aos 3, 7 e 14 dias após a extração. O qRT-PCR foi utilizado para avaliar a expressão de Tnfrsf11a (RANK), Tnfsf11 (RANKL) e Tnfrsf11b (OPG). Os dados foram submetidos à análise estatística usando two-way ANOVA seguido do teste de Bonferroni (α=0,05). Houve um aumento na expressão dos genes RANK, RANKL e OPG ao longo do tempo de cicatrização no grupo Exp I em comparação com o grupo controle. O grupo Exp II mostrou uma expressão diminuída de todos os genes ao longo do tempo, enquanto a expressão dos genes do grupo Exp III foi superior aos valores observados em Exp II, mas inferiores aos valores do grupo Controle e Exp I ao longo do tempo. Os resultados deste estudo concluíram que o LLLT tem um efeito positivo, enquanto a fumaça de cigarro possui efeito prejudicial na expressão gênica de RANK, RANKL e OPG no processo de remodelação óssea.

6.
Arch Oral Biol ; 110: 104604, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31756673

RESUMO

OBJECTIVE: The present study evaluated the association between genetic variants in WNT3A and WNT11, and palatal rugae phenotypes. DESIGN: Eighty-five biological unrelated orthodontic patients were included. Dental casts were assessed and data regarding the length, shape, direction and unification of rugae were recorded. The individuals were subsequently classified for each of the following rugae traits: total amount of rugae; bilateral symmetry in the amount, length and shape of the rugae; presence of secondary or fragmentary rugae; presence of unifications; predominant shape; and, direction of the rugae. Genetic variants in WNT3A (rs708111) and WNT11 (rs1533767) were genotyped by real-time PCR. Genotype and allele distributions were compared with an established alpha of 5 %. RESULTS: The wavy and curve rugae were the most common. Genotype/phenotype analyses identified that the presence of the rs708111 A allele (OR = 2.2, 95 % CI: 1.1-4.4, p = 0.01) and the rs1533767 G allele (OR = 2.3, 95 % CI: 1.0-5.3, p = 0.05) increased in more than two times the chance of having bilateral asymmetry in the amount of the rugae. In the recessive model, individuals carrying two risk alleles (AA) of WNT3A rs708111 had a higher risk of presenting this phenotype. SNP-SNP interaction analysis revealed that individuals carrying one rs708111 A allele and rs1533767 G allele showed even a higher chance of having bilateral asymmetry in the amount of rugae (OR = 5.6, 95 % CI: 1.1-28.8, p = 0.03). No associations were identified for other rugae phenotype (p > 0.05). CONCLUSION: Genetic variants in WNT3A and WNT11 were associated with the left-right asymmetry in the amount of palatal rugae.


Assuntos
Palato Duro , Proteínas Wnt , Via de Sinalização Wnt , Proteína Wnt3A/genética , Variação Genética , Genótipo , Humanos , Mucosa Bucal , Palato Duro/anatomia & histologia , Fenótipo , Proteínas Wnt/genética , Via de Sinalização Wnt/genética
7.
Dental Press J Orthod ; 23(1): 97-107, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29791689

RESUMO

Tooth transposition is one of the most difficult dental anomalies to treat in the dental clinic. Several factors must be taken into account with a view of making the best decision. The aim of this study was to discuss treatment modalities for tooth transposition, their advantages and disadvantages. Additionally, it aims at presenting a clinical case of transposition between canine and lateral incisor in the upper quadrant on the right side. The treatment of choice was extraction of one transposed tooth. A multidisciplinary approach involving Orthodontics, Cosmetic Dentistry, and Periodontology was necessary to allow proper esthetic and functional outcomes to be achieved.


Assuntos
Ortodontia Corretiva , Erupção Ectópica de Dente/terapia , Adolescente , Feminino , Humanos , Planejamento de Assistência ao Paciente , Radiografia Panorâmica , Erupção Ectópica de Dente/diagnóstico por imagem
8.
Dental press j. orthod. (Impr.) ; 23(1): 97-107, Jan.-Feb. 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-891121

RESUMO

ABSTRACT Tooth transposition is one of the most difficult dental anomalies to treat in the dental clinic. Several factors must be taken into account with a view of making the best decision. The aim of this study was to discuss treatment modalities for tooth transposition, their advantages and disadvantages. Additionally, it aims at presenting a clinical case of transposition between canine and lateral incisor in the upper quadrant on the right side. The treatment of choice was extraction of one transposed tooth. A multidisciplinary approach involving Orthodontics, Cosmetic Dentistry, and Periodontology was necessary to allow proper esthetic and functional outcomes to be achieved.


RESUMO A transposição é considerada uma das anomalias dentárias mais difíceis de se administrar clinicamente. Vários fatores devem ser considerados para que a melhor decisão seja tomada. Neste artigo, o objetivo foi discutir as modalidades de tratamento da transposição dentária, suas vantagens e desvantagens, e apresentar um caso clínico de transposição entre canino e incisivo lateral na hemiarcada superior direita. A opção terapêutica de escolha foi a extração de um dos dentes transpostos, tendo-se a necessidade de uma abordagem multidisciplinar com a participação da Ortodontia, Dentística e Periodontia, a fim de que adequados resultados estéticos e funcionais fossem alcançados.

9.
Braz. dent. j ; 28(3): 296-300, May-June 2017. graf
Artigo em Inglês | LILACS | ID: biblio-888648

RESUMO

Abstract The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (a=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME.


Resumo O objetivo deste estudo foi avaliar a sinalização osteoclastogenese na sutura palatina após a expansão rápida da maxila (ERM) em ratos. Um total de 30 ratos Wistar machos foram divididos aleatoriamente em dois grupos com 15 animais cada: controle (C) e grupo ERM. ERM foi realizada através da inserção de um anel de metal circular de 1,5 mm de espessura entre os incisivos superiores. Os animais foram sacrificados aos 3, 7 e 10 dias após a RME. qRT-PCR foi utilizado para avaliar a expressão de Tnfsf11 (RANKL), Tnfrsf11a (RANK) e TNFRSF11b (OPG). Os dados foram submetidos à análise de variância de duas vias, seguido pelo teste de Tukey (a=0,05). Houve uma regulação positiva de genes RANK e RANKL aos 7 e 10 dias e uma regulação positiva do gene OPG aos 3 e 7 dias de tratamento. Curiosamente, foi observado um aumento na expressão de todos os genes ao longo do tempo nos grupos ERM e C. O RANKL/OPG mostrou um aumento na sinalização favorecendo a reabsorção óssea no ERM em comparação com o C nos períodos de 3 e 7 dias. Foi observada uma sinalização contra a reabsorção óssea, assim como, uma regulação favorável da expressão do gene OPG no grupo ERM, comparado ao grupo C aos 10 dias. Os resultados deste estudo permitem concluir que o sistema RANK, RANK-L e OPG participa de remodelação óssea após a ERM.


Assuntos
Animais , Masculino , Maxila/cirurgia , Osteogênese , Osteoprotegerina/genética , Técnica de Expansão Palatina/instrumentação , Ligante RANK/genética , Receptor Ativador de Fator Nuclear kappa-B/genética , Remodelação Óssea , Expressão Gênica , Maxila/metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Regulação para Cima , Cicatrização
10.
Braz Dent J ; 28(3): 296-300, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29297549

RESUMO

The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (a=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME.


Assuntos
Maxila/cirurgia , Osteogênese , Osteoprotegerina/genética , Técnica de Expansão Palatina/instrumentação , Ligante RANK/genética , Receptor Ativador de Fator Nuclear kappa-B/genética , Animais , Remodelação Óssea , Expressão Gênica , Masculino , Maxila/metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Regulação para Cima , Cicatrização
11.
Braz Oral Res ; 30(1): e115, 2016 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-27901199

RESUMO

This study aimed to perform a systematic review and meta-analysis to evaluate the effectiveness of chlorhexidine varnish on the reduction of caries incidence during fixed orthodontic treatment. The literature searches involved The Cochrane Library, Medline, Scopus, OpenSigle databases and manual searches. The search on OpenSigle did not produce any additional articles. Clinical studies conducted in patients with orthodontic fixed appliances that used professional application of chlorhexidine varnish were included. The effect-size was calculated and a meta-analysis was performed. From 182 abstracts, a total of six articles fulfilled the inclusion criteria. After reading the full articles, one was excluded because of lack of a control group. Three articles were used for continuous data analysis, and two articles were used for the dichotomous data analysis. The pooled meta-analysis with continuous data demonstrated chlorhexidine varnish effectiveness on caries reduction (p = 0.003), with a mean difference and confidence interval of -1.49 [-2.47, -0.51]. On the basis of the pooled meta-analysis of continuous data, we were able to conclude that professional application of chlorhexidine varnish is effective in caries incidence reduction during fixed orthodontic treatment.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Cariostáticos/uso terapêutico , Clorexidina/uso terapêutico , Cárie Dentária/prevenção & controle , Aparelhos Ortodônticos/microbiologia , Adolescente , Criança , Feminino , Humanos , Masculino , Reprodutibilidade dos Testes , Resultado do Tratamento
12.
Braz. oral res. (Online) ; 30(1): e115, 2016. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-951950

RESUMO

Abstract This study aimed to perform a systematic review and meta-analysis to evaluate the effectiveness of chlorhexidine varnish on the reduction of caries incidence during fixed orthodontic treatment. The literature searches involved The Cochrane Library, Medline, Scopus, OpenSigle databases and manual searches. The search on OpenSigle did not produce any additional articles. Clinical studies conducted in patients with orthodontic fixed appliances that used professional application of chlorhexidine varnish were included. The effect-size was calculated and a meta-analysis was performed. From 182 abstracts, a total of six articles fulfilled the inclusion criteria. After reading the full articles, one was excluded because of lack of a control group. Three articles were used for continuous data analysis, and two articles were used for the dichotomous data analysis. The pooled meta-analysis with continuous data demonstrated chlorhexidine varnish effectiveness on caries reduction (p = 0.003), with a mean difference and confidence interval of −1.49 [−2.47, −0.51]. On the basis of the pooled meta-analysis of continuous data, we were able to conclude that professional application of chlorhexidine varnish is effective in caries incidence reduction during fixed orthodontic treatment.

13.
Dental press j. orthod. (Impr.) ; 17(1): 101-107, Jan.-Feb. 2012. ilus, tab
Artigo em Inglês | LILACS-Express | ID: lil-623177

RESUMO

INTRODUCTION: Rapid maxillary expansion (RME) for the treatment of maxillary deficiency and posterior crossbite may induce changes in the vertical dimension. Expanders with occlusal splints have been developed to minimize unwanted vertical effects. OBJECTIVE: This preliminary study used cephalometri radiographs to evaluate the vertical effects of RME using a Hyrax appliance in children with maxillary deficiency. METHOD: Twenty-six patients (11 boys; mean age = 8 years and 5 months) with maxillary deficiency and posterior crossbite were treated using a Hyrax appliance with an acrylic occlusal splint. Radiographs and cephalometric studies were performed before the beginning of the treatment (T1) and after RME active time (T2), at a mean interval of 7 months. Results were compared with normative values. RESULTS AND CONCLUSIONS: At the end of treatment, there were no statistically significant changes, and measurements were similar to the normative values. Data showed that there were no significant effects on vertical growth, which suggests that appliances with occlusal splints may be used to correct transverse deficiencies regardless of the patient's growth pattern.

14.
Microsc Res Tech ; 75(2): 117-25, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21761492

RESUMO

This study analyzed the newly formed bone tissue after application of recombinant human BMP-2 (rhBMP-2) and P-1 (extracted from Hevea brasiliensis) proteins, 2 weeks after the creation of a critical bone defect in male Wistar rats treated or not with a low-intensity laser (GaAlAs 780 nm, 60 mW of power, and energy density dose of 30 J/cm(2)). The animals were divided into two major groups: (1) bone defect plus low-intensity laser treatment and (2) bone defect without laser irradiation. The following subgroups were also analyzed: (a) 5 µg of pure rhBMP-2; (b) 5 µg of pure P-1 fraction; (c) 5 µg of rhBMP-2/monoolein gel; (d) 5 µg of P-1 fraction/monoolein gel; (e) pure monoolein gel. Comparisons of the groups receiving laser treatment with those that did not receive laser irradiation show differences in the areas of new bone tissue. The group treated with 5 µg of rhBMP-2 and laser irradiation was not significantly different (P >0.05) than the nonirradiated group that received the same treatment. The irradiated, rhBMP-2/monoolein gel treatment group showed a lower area of bone formation than the nonirradiated, rhBMP-2/gel monoolein treatment group (P < 0.001). The area of new bone tissue in the other nonirradiated and irradiated groups was not significantly different (P > 0.05). Furthermore, the group that received the 5 µg of rhBMP-2 application showed the greatest bone formation. We conclude that the laser treatment did not interfere with the area of new bone tissue growth and that the greatest stimulus for bone formation involved application of the rhBMP-2 protein.


Assuntos
Proteína Morfogenética Óssea 2/farmacologia , Hevea/química , Terapia com Luz de Baixa Intensidade , Osteogênese/efeitos dos fármacos , Osso Parietal/efeitos dos fármacos , Proteínas de Plantas/farmacologia , Fator de Crescimento Transformador beta/farmacologia , Animais , Proteína Morfogenética Óssea 2/administração & dosagem , Glicerídeos/administração & dosagem , Humanos , Imuno-Histoquímica , Masculino , Osteogênese/efeitos da radiação , Osso Parietal/lesões , Proteínas de Plantas/administração & dosagem , Ratos , Ratos Wistar , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/farmacologia , Fraturas Cranianas/tratamento farmacológico , Fraturas Cranianas/radioterapia , Fator de Crescimento Transformador beta/administração & dosagem
15.
Eur J Orthod ; 34(3): 367-73, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21441407

RESUMO

The aim of this study was to evaluate the short- and long-term treatment effects of rapid maxillary expansion (RME) on the soft tissue facial profile of subjects treated with a modified acrylic-hyrax device. The sample comprised 10 males and 10 females in the mixed dentition. Their average age was 9.3 years ± 10 months pre-treatment (T1), with a narrow maxilla and posterior crossbite, treated with a modified fixed maxillary expander with an occlusal splint. Lateral cephalometric radiographs obtained at T1, immediately post-expansion (T2), and after retention (T3) were used to determine possible changes in the soft tissue facial profile. The means and standard deviations for linear and angular cephalometric measurements were analysed statistically using analysis of variance and Tukey's test (α = 0.05). The measurements at T2 differed significantly from those at T1 and T3. However, RME did not produce any statistically significant alteration (P > 0.05) in the soft tissue profile for any of the cephalometric landmarks evaluated when compared at T1 and T3. The use of a fixed expander associated with an occlusal splint did not cause significant alterations in the soft tissue facial profile at T3. This modified device is effective for preventing the adverse vertical effects of RME such as an increase anterior face height in patients with a crossbite.


Assuntos
Face/anatomia & histologia , Desenvolvimento Maxilofacial , Placas Oclusais , Técnica de Expansão Palatina/instrumentação , Análise de Variância , Cefalometria , Criança , Feminino , Humanos , Masculino , Estudos Prospectivos , Estatísticas não Paramétricas , Dimensão Vertical
16.
Lasers Med Sci ; 27(4): 777-83, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21842225

RESUMO

The aim of this study was to investigate the osteoblastic activity of cells derived from the midpalatal suture upon treatment with low-level laser therapy (LLLT) after rapid maxillary expansion (RME). A total of 30 rats were divided into two groups: experimental I (15 rats with RME without LLLT) and experimental II (15 rats with RME + LLLT). The rats were euthanized at 24 h, 48 h, and 7 days after RME, when the osteoblastic cells derived from the rats' midpalatal suture were explanted. These cells were cultured for periods up to 17 days, and then in vitro osteogenesis parameters and gene expression markers were evaluated. The cellular doubling time in the proliferative stage (3-7 days) was decreased in cultured cells harvested from the midpalatal suture at 24 and 48 h after RME + LLLT, as indicated by the increased growth of the cells in a culture. Alkaline phosphatase activity at days 7 and 14 of the culture was increased by LLLT in cells explanted from the midpalatal suture at 24 and 48 h and 7 days after RME. The mineralization at day 17 was increased by LLLT after RME in all periods. Results from the real-time PCR demonstrated that cells harvested from the LLLT after RME group showed higher levels of ALP, Runx2, osteocalcin, type I collagen, and bone sialoprotein mRNA than control cells. More pronounced effects on ALP activity, mineralization, and gene expression of bone markers were observed at 48 h after RME and LLLT. These results indicate that the LLLT applied after RME is able to increase the proliferation and the expression of an osteoblastic phenotype in cells derived from the midpalatal suture.


Assuntos
Fosfatase Alcalina/metabolismo , Diferenciação Celular/efeitos da radiação , Proliferação de Células/efeitos da radiação , Terapia com Luz de Baixa Intensidade/métodos , Osteoblastos/citologia , Animais , Calcificação Fisiológica , Técnicas de Cultura de Células , Expressão Gênica , Masculino , Osteoblastos/metabolismo , Osteoblastos/efeitos da radiação , Osteogênese/efeitos da radiação , Técnica de Expansão Palatina , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real
17.
RPG rev. pos-grad ; 18(1): 52-56, Jan.-Mar. 2011. tab
Artigo em Inglês | LILACS | ID: lil-679738

RESUMO

Scientific journals represent the main disclosure tools for the advancement and development of Science. The importance of scientific journals can be usually defined in several ways including its prestige, which may be reflected in its citedness as measured by the impact factor (IF), which is a classic parameter useful to researchers. Due to controversial issues involving scientific publication, the purpose of this update article was to clarify and explain what is the impact factor of a journal and how it can guide faculty advisors and postgraduate students to publish their papers. The IF of a journal describes both journal and author impacts. It is based on two elements: the numerator, which is the number of citations in the current year to any items published in a journal in the previous two years, and the denominator, which is the number of substantive articles (source items) published in the same two years. Although IF is the only measure of journalÆs quality, it is important to remember that the calculation of the IF is biased by many factors. These include factors as inclusion of review articles, commentaries, errata and letters in numerator, but not in the denominator of the equation used to calculate the IF. Impact factor is the foremost tool to measure and indirectly indicate the research quality. Although it is not a perfect method and has several limited interpretation power, it remains a valuable measurement technique for scientific evaluation.


Assuntos
Bases de Dados de Citações , Bases de Dados Bibliográficas , Fator de Impacto , Fator de Impacto de Revistas
18.
Pediatria (Säo Paulo) ; 33(2): 107-116, 2011. tab
Artigo em Português | LILACS | ID: lil-610177

RESUMO

Objetivo: Apresentar uma revisão da literatura e descrever os diferentes métodos de sedação consciente utilizados para o tratamento odontológico em pacientes com necessidades especiais e odontopediátricos, dando ênfase na terapia com benzodiazepínicos e na inalação da mistura dos gases óxido nitroso e oxigênio. Fontes Pesquisadas: Livre escolha de artigos científicos e livros relacionados à sedação consciente. Síntese dos Dados: Existem diversas técnicas usadas como recursos alternativos à anestesia geral para o controle e manejo da ansiedade, da dor, do medo e do comportamento em pacientes odontopediátricos e pacientes especiais. Estes métodos incluem modalidades do tipo psicológicas (não-farmacológicas) e farmacológicas. Contudo, em alguns pacientes os métodos não farmacológicos ainda não são suficientes para conseguir a compreensão e a cooperação desejada para possibilitar a realização do tratamento odontológico. Desta maneira, o cirurgião-dentista pode utilizar diversos recursos farmacológicos, como agentes sedativos, analgésicos e outros fármacos úteis para sedação destes pacientes. Dentre os métodos farmacológicos conhecidos de sedação consciente, os mais empregados em Odontologia são os que utilizam os benzodiazepínicos por via oral e a técnica inalatória por meio da mistura de óxido nitroso e oxigênio. Conclusões: A sedação consciente deve ser utilizada para procedimentos odontológicos específicos e situações em que o paciente se beneficie da sedação consciente como método farmacológico de abordagem no controle da ansiedade, da dor e do medo.


Objective: To perform a literature review and describe the several methods of conscious sedation used for the dental treatment in patients with special needs and children with emphasis in benzodiazepines therapy and the mixture of nitrous oxide and oxygen gases. Data Source: Free choice of pertinent literature scientific articles and books related to conscious sedation. Data Synthesis: There are several techniques as alternative resources to the general anesthesia for the control and handling of the anxiety, pain, fear and the behavior in children and patients with special needs. These methods include psychological (non-pharmacological) and pharmacological approaches. However, in several subjects, the psychological methods are not still enough to achieve the desirable cooperation and understanding to perform dental treatment. In this way, the dentist has available several pharmacological resources such as sedative, analgesic agents and other useful drugs for the handling and control of these patients. Among the most known pharmacological methods of conscious sedation, the most used in Dentistry are the oral benzodiazepines and the mixture of nitrous oxide and oxygen gases. Conclusions: Conscious sedation should be used for dental procedures and specific situations in which patients will benefit from conscious sedation as a method of approach in the pharmacological control of anxiety, pain and fear.


Assuntos
Humanos , Masculino , Feminino , Criança , Analgesia , Criança , Sedação Consciente , Odontopediatria
19.
Pediatria (Säo Paulo) ; 32(4): 288-292, out.-dez. 2010. ilus
Artigo em Português | LILACS | ID: lil-610161

RESUMO

Objetivo: Realizar breve revisão da literatura a respeito da etiologia, localização, prevalência, faixa etária, gênero, aspectos clínicos e opções de tratamentos para as mucoceles. Fontes pesquisadas: Livre escolha de artigos pertinentes da literatura relacionados a esta lesão cística. Síntese dos dados: As mucoceles são lesões comuns da mucosa bucal, originárias de glândulas salivares menores. Nos lábios inferiores, esta patologia apresenta altaprevalência, podendo aparecer na mucosa jugal, língua, palato mole, lábio superior e na região retromolar. Os aspectos clínicos da mucocele são importantes para o diagnóstico, contudo se faz necessária a análise anatomopatológica para confirmaro diagnóstico clínico, bem como para definir se a mucocele apresenta-se como um cisto de extravasamentoou de retenção mucoso. O tratamento é simples e pode ser realizado em ambiente ambulatorial, utilizando técnicas de manejo de comportamento. Conclusão: As mucoceles são lesões frequentemente encontradas na cavidade bucal de crianças e existem diversas formas de tratamentoque podem ser realizadas na clínica odontológica. É importante uma interação entre profissionais da saúde que prestam atendimento a crianças com a finalidade de identificar e encaminhar estas lesões para tratamento no consultório odontopediátrico.


Objective: To perform a brief literature review about the etiology, localization, prevalence, age, gender, clinical characteristics and options of treatmentfor mucoceles. Researched sources: Free choice of pertinent literature articles related to this cystic lesion. Data synthesis: Mucoceles are a common pathology at the oral cavity, originating from minor salivary glands. The prevalence of mucoceles is higher in the lower labial mucosa although these lesions can be found in the buccal mucosa, tongue, soft palate, upper labialand retromolar region. Clinical aspects of mucoceles are important for diagnosis, although histological evaluation is necessary to confirm clinical diagnosis and to determinate if the lesion represents a retention mucouscysts or an extravasation phenomenon. Treatment is simple and can be performed in dental office, using behavior management techniques. Conclusion: Mucoceles are common lesions found the mouth of children and several treatment alternatives can be used. Dental treatment will be more successful if there were instituted early in a multidisciplinary approach.


Assuntos
Humanos , Criança , Diagnóstico Diferencial , Mucocele/diagnóstico , Úlceras Orais
20.
Dental press j. orthod. (Impr.) ; 15(3): 62-70, jun. 2010. ilus, tab
Artigo em Inglês, Português | LILACS-Express | ID: lil-550663

RESUMO

INTRODUÇÃO: os aparelhos expansores maxilares com cobertura oclusal têm sido sugeridos para controlar o aumento na dimensão vertical da face após a expansão rápida da maxila, porém ainda não há um consenso na literatura sobre seus reais efeitos. OBJETIVO: o objetivo deste trabalho foi avaliar as alterações cefalométricas verticais e anteroposteriores associadas à expansão da maxila realizada com o aparelho expansor com cobertura oclusal. MÉTODOS: a amostra foi composta por 25 crianças, de ambos os gêneros, com idades entre 6 e 10 anos, portadoras de mordida cruzada posterior esquelética. Após a expansão maxilar, o próprio aparelho expansor foi utilizado como contenção fixa. Foram analisadas telerradiografias em norma lateral tomadas antes do início do tratamento e após a remoção do aparelho expansor. CONCLUSÃO: com base nos resultados, pôde-se concluir que o uso do aparelho expansor com cobertura oclusal não alterou significativamente as medidas cefalométricas verticais e anteroposteriores das crianças.


INTRODUCTION: Bonded rapid maxillary expansion appliances have been suggested to control increases in the vertical dimension of the face after rapid maxillary expansion but there is still no consensus in the literature concerning its actual effectiveness. OBJECTIVE: The purpose of this study was to evaluate the vertical and anteroposterior cephalometric changes associated with maxillary expansion performed using bonded rapid maxillary expansion appliances. METHODS: The sample consisted of 25 children of both genders, aged between 6 and 10 years old, with skeletal posterior crossbite. After maxillary expansion, the expansion appliance itself was used for fixed retention. Were analyzed lateral teleradiographs taken prior to treatment onset and after removal of the expansion appliance. CONCLUSION: Based on the results, it can be concluded that the use of bonded rapid maxillary expansion appliance did not significantly alter the children's vertical and anteroposterior cephalometric measurements.

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