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Int J Biol Macromol ; 131: 10-18, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-30851325


Marine bacteria secrete exopolysaccharides (EPS) with unique structural and functional properties and serve as a source of newer bioactive biopolymers. This study reports an EPS produced by a marine bacterium identified as Alteromonas sp. PRIM-28 for its bioactivities. The EPS was characterised using standard methods and tested for its bioactivities using in vitro models. EPS-A28 is an anionic heteropolysaccharide with a molecular weight of 780 kDa and exists as triple helical structure in aqueous solution. Monosaccharide composition is mannuronic acid, glucose and N-acetyl glucosamine repeating units in the ratio 1:3.67:0.93. The FT-IR spectra showed the presence of sulphate, phosphate and uronic acid residues. The thermal analysis showed partial degradation of the EPS-A28 at 190 °C and 40% of residues were stable up to 800 °C. It showed biocompatibility and induced proliferation and migration of dermal fibroblasts (HDF) and keratinocytes. EPS-A28 could increase the S-phase of cell cycle. The proliferative property of the EPS-A28 was established by the increased expression of fibroblast proliferation marker (Ki-67) also its capability of binding to cell surface. It also induced nitric oxide and arginase synthesis in macrophages. These findings suggest that EPS-A28 can be potentially used as a multifunctional bioactive polymer in wound care.

Alteromonas/química , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/farmacologia , Cicatrização/efeitos dos fármacos , Animais , Biomarcadores , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fibroblastos , Humanos , Queratinócitos , Camundongos , Peso Molecular , Monossacarídeos , Células RAW 264.7 , Termodinâmica
Mol Biol Rep ; 45(6): 2857-2867, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30094529


Wound is a growing healthcare challenge affecting several million worldwide. Lifestyle disorders such as diabetes increases the risk of wound complications. Effective management of wound is often difficult due to the complexity in the healing process. Addition to the conventional wound care practices, the bioactive polymers are gaining increased importance in wound care. Biopolymers are naturally occurring biomolecules synthesized by microbes, plants and animals with highest degree of biocompatibility. The bioactive properties such as antimicrobial, immune-modulatory, cell proliferative and angiogenic of the polymers create a microenvironment favorable for the healing process. The versatile properties of the biopolymers such as cellulose, alginate, hyaluronic acid, collagen, chitosan etc have been exploited in the current wound care market. With the technological advances in material science, regenerative medicine, nanotechnology, and bioengineering; the functional and structural characteristics of biopolymers can be improved to suit the current wound care demands such as tissue repair, restoration of lost tissue integrity and scarless healing. In this review we highlight on the sources, mechanism of action and bioengineering approaches adapted for commercial exploitation.

Biopolímeros/uso terapêutico , Engenharia Tecidual/métodos , Cicatrização/efeitos dos fármacos , Alginatos/uso terapêutico , Animais , Celulose/uso terapêutico , Quitosana/uso terapêutico , Colágeno/uso terapêutico , Humanos , Ácido Hialurônico/uso terapêutico , Medicina Regenerativa/métodos , Pele/metabolismo , Transplante de Pele/métodos