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1.
Vet Res ; 50(1): 50, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31227007

RESUMO

Nervous necrosis virus (NNV), Genus Betanodavirus, is the causative agent of viral encephalopathy and retinopathy (VER), a neuropathological disease that causes fish mortalities worldwide. The NNV genome is composed of two single-stranded RNA molecules, RNA1 and RNA2, encoding the RNA polymerase and the coat protein, respectively. Betanodaviruses are classified into four genotypes: red-spotted grouper nervous necrosis virus (RGNNV), striped jack nervous necrosis virus (SJNNV), barfin flounder nervous necrosis virus (BFNNV) and tiger puffer nervous necrosis virus (TPNNV). In Southern Europe the presence of RGNNV, SJNNV and their natural reassortants (in both RNA1/RNA2 forms: RGNNV/SJNNV and SJNNV/RGNNV) has been reported. Pathology caused by these genotypes is closely linked to water temperature and the RNA1 segment encoding amino acids 1-445 has been postulated to regulate viral adaptation to temperature. Reassortants isolated from sole (RGNNV/SJNNV) show 6 substitutions in this region when compared with the RGNNV genotype (positions 41, 48, 218, 223, 238 and 289). We have demonstrated that change of these positions to those present in the RGNNV genotype cause low and delayed replication in vitro when compared with that of the wild type strain at 25 and 30 °C. The experimental infections confirmed the impact of the mutations on viral replication because at 25 °C the viral load and the mortality were significantly lower in fish infected with the mutant than in those challenged with the non-mutated virus. It was not possible to challenge fish at 30 °C because of the scarce tolerance of sole to this temperature.


Assuntos
Substituição de Aminoácidos , Linguados/virologia , Temperatura Alta , Mutação/genética , Nodaviridae/genética , Adaptação Fisiológica , Animais , Encéfalo/virologia , Linhagem Celular , Mutagênese Sítio-Dirigida , Nodaviridae/fisiologia , Replicação Viral
2.
Eur J Intern Med ; 65: 69-77, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31076345

RESUMO

BACKGROUND: Little is known about the prevalence and impact of risk of malnutrition on short-term mortality among seniors presenting with acute heart failure (AHF) in emergency setting. The objective was to determine the impact of risk of malnutrition on 30-day mortality risk among older patients who attended in Emergency Departments (EDs) for AHF. MATERIAL AND METHODS: We performed a secondary analysis of the OAK-3 Registry including all consecutive patients ≥65 years attending in 16 Spanish EDs for AHF. Risk of malnutrition was defined by the Mini Nutritional Assessment Short Form (MNA-SF) < 12 points. Unadjusted and adjusted logistic regression models were used to assess the association between risk of malnutrition and 30-day mortality. RESULTS: We included 749 patients (mean age: 85 (SD 6); 55.8% females). Risk of malnutrition was observed in 594 (79.3%) patients. The rate of 30-day mortality was 8.8%. After adjusting for MEESSI-AHF risk score clinical categories (model 1) and after adding all variables showing a significantly different distribution among groups (model 2), the risk of malnutrition was an independent factor associated with 30-day mortality (adjusted OR by model 1 = 3.4; 95%CI 1.2-9.7; p = .020 and adjusted OR by model 2 = 3.1; 95%CI 1.1-9.0; p = .033) compared to normal nutritional status. CONCLUSIONS: The risk of malnutrition assessed by the MNA-SF is associated with 30-day mortality in older patients with AHF who were attended in EDs. Routine screening of risk of malnutrition may help emergency physicians in decision-making and establishing a care plan.

3.
Diaeta (B. Aires) ; 36(165): 28-36, dic. 2018. tab
Artigo em Espanhol | LILACS-Express | ID: biblio-1011983

RESUMO

Introducción: la parálisis cerebral (PC) es la afección neuromotriz más común de la primera infancia siendo la principal causa de discapacidad física en niños. Esta patología afecta el estado nutricional de quienes la padecen y, a su vez, la malnutrición exacerba las alteraciones neurológicas preexistentes. Objetivos: determinar la distribución de frecuencias del tipo de alimentación y el tipo de fórmula o leche seleccionada en los pacientes con diagnóstico de PC con soporte enteral exclusivamente. Determinar el aporte calórico, proteico, de fibra, líquidos, calcio y hierro. Determinar la concordancia entre el estado nutricional de los pacientes y el percibido por sus cuidadores. Materiales y Método: estudio observacional, transversal, descriptivo y analítico. Muestreo consecutivo, por conveniencia. Resultados: la muestra quedó conformada por 30 pacientes y sus cuidadores. El 70% de la muestra utilizaba fórmula y/o leche, el 3% alimentos modificados y el 27% realizaba una alimentación mixta; el tipo de fórmula más utilizado fue la infantil (33%). El 60% de la muestra fue normopeso, 33% bajo peso y el 7% tuvo exceso de peso. El aporte calórico promedio fue de 11,1 kcal/cm (DS + 6,5 kcal/cm) en los niños con bajo peso, 11,6 kcal/cm (DS + 6,4 kcal/cm) en aquellos con normopeso y 9,8 kcal/cm (+ 2,4 kcal/cm) en los niños con exceso de peso; no se observaron diferencias según estado nutricional (p=0,13). El 90% cubrió el requerimiento de proteínas, el 80% el de hierro, 73% el hídrico, el 70% el de calcio y un 3% el de fibra. Se halló concordancia moderada entre el estado nutricional real del niño y el percibido por su cuidador. (kappa 0,44; p=0,0013; IC95 0,22-0,62%). Conclusión: Los pacientes con PC requieren el acompañamiento de Licenciados en nutrición para adecuar el aporte a sus requerimientos nutricionales considerando las características de la PC y su tratamiento.


Introduction: cerebral palsy (CP) is the most common neuromotor condition during early childhood, being the main cause of physical disability in children. This pathology affects the nutritional status of those affected, and in turn, malnutrition exacerbates pre-existing neurological disorders. Objectives: to determine the distributional frequency of feeding type and milk or formula type selected within patients diagnosed with PC and enteral support exclusively. To determine the caloric, protein, fiber, fluids, calcium and iron intake. To determine concordance between the nutritional status of patients and that perceived by their caregivers. Materials and Methods: an observational, transversal, prospective, descriptive, analytical study with a consecutive sampling was conducted. Results: the study included 30 patients and their caregivers. 70% of the sample used formula and/or milk, 3% modified foods and 27% a mixed diet; infant formula was the one mostly used (33%). 60% of the sample was normal weight, 33% low weight and 7% overweight. The average caloric intake was 11.1 kcal/cm (SD +6.5 kcal/cm) in children with low weight, 11.6 kcal/cm (SD +6.4 kcal/cm) in those with normal weight and 9.8 kcal/cm (SD +2.4 kcal/cm) in children with overweight; no differences were observed following nutritional status (p=0.13). 90% covered protein requirement, 80% iron, 73% water, 70% calcium and 3% fiber. Moderate agreement was found between the real nutritional status of the child and that perceived by the caregiver (kappa 0.44, p=0.0013, IC95 0.22-0.62%). Conclusion: patients with CP require the accompaniment of graduates in nutrition to adapt nutritional requirements, taking into consideration the characteristics of the CP and its treatment.

4.
Vet Res ; 49(1): 86, 2018 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-30185222

RESUMO

Nervous necrosis virus (NNV), G. Betanodavirus, is the causative agent of viral encephalopathy and retinopathy, a disease that causes mass mortalities in a wide range of fish species. Betanodaviruses are neurotropic viruses and their replication in the susceptible fish species seems to be almost entirely restricted to nerve tissue. However, none of the cell lines used for NNV propagation has a nervous origin. In this study, first we established a protocol for the primary culture of neurons from Senegalese sole, which made it possible to further study virus-host cell interactions. Then, we compared the replication of three NNV strains with different genotypes (SJNNV, RGNNV and a RGNNV/SJNNV reassortant strain) in sole neuron primary cultures and E-11 cells. In addition, to study how two amino acid substitutions at the c-terminal of the capsid protein (positions 247 and 270) affect the binding to cell receptors, a recombinant strain was also tested. The results show that sole neural cells enabled replication of all the tested NNV strains. However, the recombinant strain shows a clearly delayed replication when compared with the wt strain. This delay was not observed in virus replicating in E-11 cells, suggesting a viral interaction with different cell receptors. The establishment of a sole primary neuronal culture protocol provides an important tool for research into betanodavirus infection in sole.


Assuntos
Proteínas do Capsídeo/genética , Doenças dos Peixes/virologia , Linguados , Neurônios/virologia , Nodaviridae/fisiologia , Infecções por Vírus de RNA/veterinária , Replicação Viral/genética , Animais , Proteínas do Capsídeo/metabolismo , Células Cultivadas/virologia , Mutação , Cultura Primária de Células/métodos , Cultura Primária de Células/veterinária , Infecções por Vírus de RNA/virologia
5.
Int J Mol Sci ; 16(12): 30470-82, 2015 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-26703581

RESUMO

UNLABELLED: The aim of this study is to describe the results obtained after growth hormone (GH) treatment and neurorehabilitation in a young man that suffered a very grave traumatic brain injury (TBI) after a plane crash. METHODS: Fifteen months after the accident, the patient was treated with GH, 1 mg/day, at three-month intervals, followed by one-month resting, together with daily neurorehabilitation. Blood analysis at admission showed that no pituitary deficits existed. At admission, the patient presented: spastic tetraplegia, dysarthria, dysphagia, very severe cognitive deficits and joint deformities. Computerized tomography scanners (CT-Scans) revealed the practical loss of the right brain hemisphere and important injuries in the left one. Clinical and blood analysis assessments were performed every three months for three years. Feet surgery was needed because of irreducible equinovarus. RESULTS: Clinical and kinesitherapy assessments revealed a prompt improvement in cognitive functions, dysarthria and dysphagia disappeared and three years later the patient was able to live a practically normal life, walking alone and coming back to his studies. No adverse effects were observed during and after GH administration. CONCLUSIONS: These results, together with previous results from our group, indicate that GH treatment is safe and effective for helping neurorehabilitation in TBI patients, once the acute phase is resolved, regardless of whether or not they have GH-deficiency (GHD).


Assuntos
Acidentes Aeronáuticos , Lesões Encefálicas/tratamento farmacológico , Hormônio do Crescimento/uso terapêutico , Adolescente , Lesões Encefálicas/etiologia , Lesões Encefálicas/reabilitação , Hormônio do Crescimento/administração & dosagem , Humanos , Cinesiologia Aplicada , Masculino
6.
Rev. fac. cienc. méd. (Impr.) ; 10(1): 30-37, ene.-jun. 2013. graf
Artigo em Espanhol | LILACS | ID: lil-750073

RESUMO

Los Trastornos Hipertensivos del Embarazo (THE) se caracterizan por presión arterial sistólica mayor o iguala 140 mm Hg o diastólica mayor o igual a 90 mm Hg. Representan la segunda causa de mortalidad materna en Honduras y provocan elevada morbimortalidad perinatal. Objetivo: Describir los datos clínico-epidemiológicos, evaluación, manejo y complicaciones materno-fetales de los trastornos hipertensivos del embarazo en el Hospital Escuela. Métodos:Estudio transversal, descriptivo, realizado del 16 de marzo al 26 de julio de 2010, se revisaron los expedientes clínicos de mujeres con diagnóstico de trastorno hipertensivo del embarazo, excluyendo los casos de hipertensión gestacional. Resultados: La prevalencia de los trastornos hipertensivos del embarazo es 7.8%, de estos 47% presentaron preeclampsia leve y 38% preeclampsia severa; se les realizó inducción y cesárea al 40% respectivamente. Se practicó cardiotocografía en reposo al 10.4% y prueba de provocación con oxitocina al 9%; se presentó crisis hipertensiva en el 35% de los casos. La principal complicación materna fue el Síndrome de HELLP y la principal complicación neonatal el Síndrome de Distrés Respiratorio (SDR), aproximadamente el 50% de los recién nacidos fueron pequeños para la edad gestacional. Se presentaron 8 muertes fetales, 11 muertes neonatales y una muerte materna. Conclusión: las mujeres con THE tienen el doble de riesgo de terminar su embarazo en cesárea comparado con las mujeres sin este trastorno. La mortalidad materna se asocia con eclampsia y Síndrome deHELLP (por sus siglas en inglés: H de hemolysis, EL de elevated liver enzymes y LP de low platelet count); la mortalidad perinatal con preeclampsia severa, APGAR menor a 7 al primer minuto, prematurez y restricción del crecimiento intrauterino...


Assuntos
Humanos , Feminino , Gravidez , Hipertensão Induzida pela Gravidez/diagnóstico , Complicações do Trabalho de Parto , Pré-Eclâmpsia , Mortalidade Materna/tendências , Fatores de Risco
7.
Rio de Janeiro; IPEA; 2003. 52 p. (Texto para Discussão / IPEA).
Monografia em Português | LILACS, ECOS | ID: biblio-991934

RESUMO

Examina o projeto de reforma do Estado empreendido pelo governo Fernando Henrique, representado pela criação de agências regulatórias após a privatização de serviços públicos. Constata-se a existência de dois tipos distintos de agências regulatórias: o primeiro, representado pelas agências de governo, que executam diretrizes de governo, e o segundo, equivalentes ao modelo anglo-saxão, que podem ser denominados agências de Estado e que regulam a oferta de serviços públicos por meio de aplicação de legislação própria específica. Apresenta propostas de aperfeiçoamento institucional do modelo.


Assuntos
Regulamentação Governamental , Saúde Suplementar , Serviços de Saúde , Tomada de Decisões , Vigilância Sanitária , Brasil
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