Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 65
Filtrar
1.
Braz. j. biol ; 83: e246450, 2023.
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1278532
2.
Braz. j. biol ; 83: e246579, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1278558

RESUMO

Abstract Water magnetization and geoprocessing are increasingly utilized tools in weed management. Our objective was to study the influence of water magnetization on herbicide efficiency and to verify whether there is a relationship between control scores and the normalized difference vegetation index (NDVI). In the laboratory experiment, water was subjected to magnetization and evaluated with respect to four characteristics. In the field experiment, plots of Brachiaria grass were subjected to treatments in a factorial scheme (6 × 2 + 1). Six herbicidal factors (doses of glyphosate and glyphosate + 2,4-D) and the magnetization or absence of magnetization of the spray solution were evaluated and compared against the control treatment (without spraying). Weed control assessments were carried out six times. Images were obtained using an embedded multispectral camera to determine the NDVI values. Data related to water characteristics were analyzed using the t test. Weed control and NDVI data were subjected to analysis of variance and are presented in regression graphs. Dispersion analysis of NDVI data was performed according to the control scores. The magnetization process decreased the pH of the water and increased the surface tension, but it did not influence the control scores or the NDVI. As the glyphosate dose was increased, the control scores were higher and the NDVI values were lower. Magnetized water did not affect the biological efficiency of the herbicides, and there was a strong correlation between the control scores and the NDVI values.


Resumo A magnetização da água e o geoprocessamento são ferramentas cada vez mais utilizadas no manejo de ervas daninhas. Nosso objetivo foi estudar a influência da magnetização da água na eficiência do herbicida e verificar se existe uma relação entre os escores de controle e o índice de vegetação por diferença normalizada (NDVI). No experimento de laboratório, a água foi submetida à magnetização e avaliada em relação a quatro características. No experimento de campo, parcelas de capim-braquiária foram submetidas a tratamentos em esquema fatorial (6 × 2 + 1). Seis fatores herbicidas (doses de glyphosate e glyphosate + 2,4-D) e a magnetização ou ausência de magnetização da calda foram avaliados e comparados com o tratamento controle (sem pulverização). Avaliações de controle de plantas daninhas foram realizadas seis vezes. As imagens foram obtidas usando uma câmera multiespectral incorporada para determinar os valores de NDVI. Os dados relacionados às características da água foram analisados ​​por meio do teste t. O controle de plantas daninhas e os dados de NDVI foram submetidos à análise de variância e são apresentados em gráficos de regressão. A análise de dispersão dos dados NDVI foi realizada de acordo com os escores de controle. O processo de magnetização diminuiu o pH da água e aumentou a tensão superficial, mas não influenciou nos escores de controle ou no NDVI. Conforme a dose de glyphosate foi aumentada, os escores de controle foram maiores e os valores de NDVI foram menores. A água magnetizada não afetou a eficiência biológica dos herbicidas, e houve uma forte correlação entre os escores de controle e os valores de NDVI.

3.
Braz J Biol ; 83: e246579, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34190763

RESUMO

Water magnetization and geoprocessing are increasingly utilized tools in weed management. Our objective was to study the influence of water magnetization on herbicide efficiency and to verify whether there is a relationship between control scores and the normalized difference vegetation index (NDVI). In the laboratory experiment, water was subjected to magnetization and evaluated with respect to four characteristics. In the field experiment, plots of Brachiaria grass were subjected to treatments in a factorial scheme (6 × 2 + 1). Six herbicidal factors (doses of glyphosate and glyphosate + 2,4-D) and the magnetization or absence of magnetization of the spray solution were evaluated and compared against the control treatment (without spraying). Weed control assessments were carried out six times. Images were obtained using an embedded multispectral camera to determine the NDVI values. Data related to water characteristics were analyzed using the t test. Weed control and NDVI data were subjected to analysis of variance and are presented in regression graphs. Dispersion analysis of NDVI data was performed according to the control scores. The magnetization process decreased the pH of the water and increased the surface tension, but it did not influence the control scores or the NDVI. As the glyphosate dose was increased, the control scores were higher and the NDVI values were lower. Magnetized water did not affect the biological efficiency of the herbicides, and there was a strong correlation between the control scores and the NDVI values.


Assuntos
Dessecação , Herbicidas , Ácido 2,4-Diclorofenoxiacético , Glicina/análogos & derivados , Controle de Plantas Daninhas
5.
Arq. bras. med. vet. zootec. (Online) ; 72(1): 79-86, Jan.-Feb. 2020. tab, ilus
Artigo em Português | LILACS, VETINDEX | ID: biblio-1088942

RESUMO

Dos atendimentos ortopédicos realizados em aves no HCV-UFRGS, 86% são fraturas, sendo aproximadamente 30% delas cominutivas com perda óssea expressiva, justificando a importância da utilização de enxertos em fraturas de aves. O objetivo deste trabalho foi avaliar dois aloenxertos e enxerto sintético de hidroxiapatita em defeito ósseo de galinhas. Utilizaram-se 30 galinhas separadas em três grupos: aloenxerto congelado em ultra-freezer (GUF), aloenxerto congelado em nitrogênio líquido (GNL) e enxerto sintético de hidroxiapatita deficiente em cálcio (GHA). Nos três grupos, os enxertos foram aplicados com placas e parafusos bloqueados de 2mm na ulna direita das aves, avaliando-se a evolução por meio de exames radiográficos até serem completados 90 dias de pós-operatório e o resultado final mediante exame histológico. A média e desvio-padrão relacionando o tempo de consolidação óssea radiográfica foi: GNL 61,67±21,79 dias (90% de consolidação), GUF 47,14±13,50 dias (70% de consolidação) e GHA 70±18,17 dias (60% de consolidação). Houve diferença significativa no tempo de consolidação óssea entre o GUF e o GHA. Histologicamente, os enxertos do GUF foram os que estavam em consolidação mais avançada. Os aloenxertos do GNL foram superiores no preenchimento de falha óssea ulnar de galinhas.(AU)


Of the orthopedic visits performed on birds at HCV-UFRGS, 86% are fractures, and approximately 30% of them are comminuted with expressive bone loss, justifying the importance of the use of grafts in bird fractures. The objective of this work was to test two allografts and a synthetic HADC graft on finishing in Gallus gallus domesticus. 30 laying hens were used, divided in three groups: frozen allograft in ultrafreezer (UFG); frozen allograft in liquid nitrogen (LNG); calcium deficient synthetic hydroxyapatite graft (HAG). The three graft groups were exposed to serial radiographs until the 90 postoperative days, as well as the histological examination at the end of the experiment were: LNG 61.67±21.79 days (90% consolidation), UFG 47.14±13.50 days (70% consolidation) and HAG 70±18.17 days (60% consolidation). There was a significant difference in bone healing time between GUF and GHA. Histologically, GUF grafts were the ones that were in the most advanced consolidation. LNG allografts were superior in filling ulnar bone failure of fowl.(AU)


Assuntos
Animais , Fraturas da Ulna/diagnóstico por imagem , Galinhas/cirurgia , Transplante Ósseo/veterinária , Aloenxertos , Hidroxiapatitas/uso terapêutico
6.
Neotrop Entomol ; 49(1): 1-11, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31749122

RESUMO

Insecticidal gene silencing by RNA interference (RNAi) involves a post-transcriptional mechanism with great potential for insect control. Here, we aim to summarize the progress on RNAi research toward control of insect pests in the Neotropical region and discuss factors determining its efficacy and prospects for pest management. We include an overview of the available RNAi information for Neotropical pests in the Lepidoptera, Coleoptera, Diptera, and Hemiptera orders. Emphasis is put on significant findings in the use of RNAi against relevant Neotropical pests, including diamondback moth (Plutella xylostella L.), Asian citrus psyllid (Diaphorina citri Kuwayama), and the cotton boll weevil (Anthonomus grandis Boheman). We also examine the main factors involved in insecticidal RNAi efficiency and major advances to improve screening of lethal genes, formulation, and delivery. Few studies detail resistance mechanisms to RNAi, demonstrating a need for more research. Advances in formulation, delivery, and resistance management tools for insecticidal RNAi in the Neotropics can provide a basis for efficient field application.


Assuntos
Hemípteros/genética , Controle de Insetos/métodos , Mariposas/genética , Interferência de RNA , Gorgulhos/genética , Animais , América Central , Genes Letais , América do Sul , Clima Tropical
7.
Arq. bras. med. vet. zootec. (Online) ; 70(2): 511-516, mar.-abr. 2018. tab, ilus
Artigo em Português | LILACS, VETINDEX | ID: biblio-910681

RESUMO

O presente estudo relata o caso de um veado-catingueiro jovem, provindo de vida livre e com histórico de atropelamento. Posteriormente à realização de exames, o animal foi diagnosticado com fratura exposta distal de tíbia (Salter-Harris tipo I) e fratura cominutiva de metáfise distal de fêmur, ambas em membro pélvico esquerdo. Para a osteossíntese da tíbia, foram utilizados pinos intramedulares de Steinmann inseridos pelos maléolos lateral e medial, de forma cruzada. A osteossíntese do fêmur foi realizada com associação de dois pinos de Steinmann, conforme técnica modificada de Rush, e placa de compressão dinâmica. As técnicas cirúrgicas adotadas foram satisfatórias para estabilização, consolidação de ambas as fraturas e retorno funcional do membro. Após 90 dias da cirurgia, o animal foi reintroduzido à natureza.(AU)


The present study reports the case of a juvenile wild brown brocket deer, with car accident history. After a complete examination, the animal was diagnosed with open distal tibial fracture (Salter-Harris type I) and comminuted fracture of the distal metaphysis of the femur, both in the left hindlimb. For the tibial osteosynthesis, Steinmann pins were used intramedullary, inserted by the lateral and medial malleolus, crosswise. The osteosynthesis of the femur was performed with a combination of two Steinmann pins, as modified Rush technique, and a dynamic compression plate. The surgical techniques used were satisfactory for stabilization, consolidation of both fractures and function return of the member. Ninety days after surgery the animal was reintroduced to nature.(AU)


Assuntos
Animais , Cães , Cervus brasilicus/antagonistas & inibidores , Fixação Interna de Fraturas/estatística & dados numéricos , Fixação Interna de Fraturas/veterinária , Fêmur/anormalidades , Tíbia
8.
Arq. bras. med. vet. zootec. (Online) ; 70(4): 1036-1044, jul.-ago. 2018. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-916262

RESUMO

Physical activity alters the cardiovascular system of dogs, depending on the exercise characteristics and the animal's physical conditioning. Little is known about the cardiovascular changes in rescue-trained dogs. This study evaluated the cardiovascular responses to a search and rescue exercise session to differentiate these alterations from cases of exhaustion or some possible pathology. Nine healthy rescue-trained dogs that trained for at least one year were used. Seven German Shepherds and two Belgian Shepherd Malinois were evaluated twice, immediately before exercise (M0) and immediately after a 20-minute training (M1). Electrocardiographic, echocardiographic, and systemic blood pressure (SBP) measurements were performed at each evaluation. Heart rate was evaluated in three moments, M0, M1 and five minutes after the end of the physical activity (M2). The results indicated that training increased oxygen demand and significantly increased cardiac output, left ventricular volume in diastole and aortic artery diameter, and the contraction force with the increased mitral annular motion without impairing systolic and diastolic cardiac functions. Heart rate values immediately and five minutes after exercise were similar to baseline values. Training did not alter SBP and the electrocardiographic parameters. The present study indicated good cardiac performance to the physical effort of rescue-trained dogs and reduced the chances of poor performance and the occurrence of sudden death caused by exercise in response to the pattern of activity performed.(AU)


A atividade física acarreta diversas mudanças no sistema cardiovascular dos cães, dependendo das características do exercício realizado e do condicionamento físico do animal. Pouco se sabe sobre as alterações cardiovasculares causadas pelo treinamento de busca, resgate e salvamento. Objetivou-se, com este estudo, avaliar as respostas cardiovasculares após uma sessão desse tipo de treino, a fim de diferenciar essas alterações de casos de exaustão ou de alguma possível patologia. Foram utilizados nove cães saudáveis em treinamento de busca, resgate e salvamento há pelo menos um ano, sendo sete da raça Pastor Alemão e dois da raça Pastor Belga Malinois. Os cães foram submetidos a dois momentos de avaliação: M0 imediatamente antes do exercício e M1 imediatamente após um treinamento de 20 minutos. Em cada avaliação, foram realizados os exames eletrocardiográfico e ecocardiográfico e a aferição da pressão arterial sistólica sistêmica. Apenas a frequência cardíaca foi avaliada em três momentos, em M0, M1 e após cinco minutos do término da atividade física (M2). Os resultados obtidos indicam que o treinamento causa um aumento na demanda de oxigênio, provocando um aumento significativo no débito cardíaco, no volume ventricular esquerdo em diástole e no diâmetro da artéria aorta, assim como um aumento em um dos parâmetros de contratilidade cardíaca (movimento anular de mitral), sem causar prejuízo às funções cardíacas sistólicas e diastólicas. Os valores da FC imediatamente após e cinco minutos após o exercício foram similares aos valores basais. A PASS e os parâmetros eletrocardiográficos não se alteraram após o treinamento. Os achados encontrados indicam um bom desempenho cardíaco ao esforço físico dos cães de busca, resgate e salvamento, reduzindo-se as chances de má performance e de ocorrência de morte súbita causada pelo exercício, em resposta ao padrão da atividade realizada.(AU)


Assuntos
Animais , Cães , Fármacos Cardiovasculares , Cães , Exercício Físico , Pressão Arterial , Eletrocardiografia/veterinária
9.
Arq. bras. med. vet. zootec. (Online) ; 70(4): 1221-1226, jul.-ago. 2018. ilus
Artigo em Português | LILACS, VETINDEX | ID: biblio-946456

RESUMO

Uma fêmea, adulta, de ouriço-cacheiro (Coendou spinosus, F. Cuvier, 1823), foi atendida com histórico de trauma medular. No exame neurológico, foi constatada paraplegia com nocicepção preservada e sinais compatíveis com choque espinhal. Exame radiográfico e mielografia apontaram presença de fratura e luxação vertebral torácica entre T10-11. O paciente foi submetido à técnica de estabilização vertebral segmentar modificada, com pinos de Steinmann e fios de cerclagem. A técnica utilizada mostrou-se eficaz na estabilização da fratura/luxação vertebral e na recuperação dos sinais neurológicos. O paciente apresentou recuperação progressiva da deambulação. Para o conhecimento dos autores, este é o primeiro relato utilizando estabilização vertebral segmentar modificada para o tratamento de fratura/luxação vertebral torácica em Coendou spinosus.(AU)


An female, adult Paraguayan hairy dwarf porcupine (Coendou spinosus, F. Cuvier, 1823) was referred to the Veterinary Hospital with clinical history of spinal cord injury. In the neurological examination, paraplegia with preserved nociception compatible with signs of spinal shock were observed. Radiographic and myelographic examinations showed evidence of a vertebral dislocation and fracture between the 10th and 11th thoracic vertebrae. The patient underwent surgical stabilization of the lesion using the modified segmental vertebral stabilization technique with Steinmann pins and cerclage wires. Under the conditions reported in this case, the technique was effective in stabilizing vertebral fracture/dislocation and in recovering neurological functions. The patient presented progressive recovery from ambulation. To the authors' knowledge, this is the first case report that performed modified segment stabilization for thoracic vertebral fracture/luxation treatment in Coendou spinosus.(AU)


Assuntos
Animais , Feminino , Fraturas Ósseas , Porcos-Espinhos/anatomia & histologia , Medula Espinal , Estabilização da Matéria Orgânica
10.
Arq. bras. med. vet. zootec. (Online) ; 70(6): 1767-1774, nov.-dez. 2018. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-970408

RESUMO

Considering that the use of tranquillizers could optimize the performance of the echocardiogram, this study aimed to evaluate the effect of protocols with acepromazine and fentanyl on the echocardiographic parameters of healthy dogs, besides their effect in systolic blood pressure (SBP), respiratory rate (RR), heart rate (HR), time spent for examination and sedation scale. Ten adult dogs were submitted to different tranquilizing protocols 20 minutes before the echocardiographic examination, totalling five treatments for each pair, performed at seven-day intervals between evaluations. The treatments were CT (control treatment), IAT (intramuscular acepromazine), OAT (oral acepromazine), FT (fentanyl) and AFT (acepromazine associated with fentanyl). In addition to the echocardiographic evaluation, SBP, degree of reassurance, duration of the exam, HR and RR in the different protocols were evaluated. There was a significant decrease of SBP in OAT. There was a significant reduction in left ventricular diameter during systole and diastole and mitral annular movement in IAT, OAT and AFT, compared with CT. There was a decrease in tricuspid annular plane systolic excursion and increase in mitral E/mitral A ratio in IAT and OAT when compared with CT. All the tranquillizer protocols studied were found to significantly reduce HR, that facilitated the echocardiographic examination.(AU)


Considerando que o uso de tranquilizantes poderia otimizar a realização do ecocardiograma, objetivou-se com este estudo avaliar o efeito da tranquilização com acepromazina e fentanil sobre os parâmetros ecocardiográficos em cães saudáveis, bem como o efeito na pressão arterial sistólica (PAS), na frequência respiratória (FR), na frequência cardíaca (FC), no tempo gasto para a realização do exame e na escala de sedação. Dez cães adultos foram submetidos a diferentes protocolos tranquilizantes, 20 minutos antes da avaliação ecocardiográfica, totalizando cinco tratamentos para cada dupla, realizados com intervalos de sete dias entre as avaliações. Os tratamentos foram: TC (tratamento controle), TAI (acepromazina intramuscular), TAO (acepromazina oral), TF (fentanil) e TAF (acepromazina associada ao fentanil). Além dos parâmetros ecocardiográficos, foram avaliados a PAS, o grau de tranquilização, o tempo de duração do exame e a FC e a FR nos diferentes protocolos. Houve diminuição significativa da PAS no TAO. Observou-se redução significativa do diâmetro do ventrículo esquerdo em sístole e diástole e do movimento anular de mitral nos protocolos TAI, TAO e TAF, comparados com o TC. Observou-se também uma redução da excursão sistólica do plano anular tricúspide e aumento da relação mitral E/mitral A nos protocolos TAI e TAO quando comparados ao TC. Todos os protocolos de tranquilização reduziram significativamente a FC, o que facilitou a realização do exame.(AU)


Assuntos
Animais , Cães , Ecocardiografia/estatística & dados numéricos , Fentanila/análise , Cães/anormalidades , Acepromazina/análise
11.
J Food Prot ; 80(10): 1605-1612, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28853626

RESUMO

International food transit is a risk to public and animal health when not subject to legal importation sanitation procedures. Due to the extensive border area, illegal food import in Brazil is a common practice, especially in Rio Grande do Sul (RS), a state that borders with Argentina and Uruguay. The objective of this study was to evaluate the profile of Brazilians living in cities in RS that border with Argentina (BR-AR) or Uruguay (BR-UR) regarding the practice of illegal import of products of animal origin and to determine associations between the population characteristics and illegal import. A questionnaire with information related to the personal profile, habits of acquisition of imported food, and knowledge of health risks deriving from the consumption of the imported products was elaborated. The questionnaire was administered in six cities in RS (three cities bordering Argentina and three cities bordering Uruguay) and responses were obtained from 744 individuals. The variables city, sex, level of education, and knowledge were subjected to the chi-square test to verify the association between these variables and food import. Part of the interviewees admitted to illegally importing products of animal origin at both BR-AR (65.17%) and BR-UR (76.28%) borders. Dairy products were the main imported goods, followed by raw and processed meat. The study revealed that illegal import is common at the frontier region of RS, especially that of products of animal origin, dairy, and raw and processed meat. Although illegal importation occurs at all the cities under study, it was higher at the BR-UR border. Also, knowledge of the health risks influences the decision to import food or not.


Assuntos
Comércio/legislação & jurisprudência , Carne , Animais , Argentina , Brasil , Cidades , Humanos , Uruguai
12.
Genet Mol Res ; 16(1)2017 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-28340267

RESUMO

Digital image analysis of seeds has been used for the identification of cultivars, determination of seed color and mechanical damage, and classification of different seed sizes. The aim of the present study was to evaluate the efficiency of digital image analysis of seeds for the quantification of genetic diversity among genotypes of inbred guava (Psidium guajava L.) families. The SAS Mini equipment, which consists of a capture module and a software program for analysis, was employed for the capture and analysis of the seed images. Different genetic diversity quantification strategies were tested using the Ward-Modified Location Model method. The set of variables related to geometry of the seeds was the largest contributor to divergence among the guava genotypes. The use of seed descriptors obtained by digital image analysis via the SAS system was efficient at quantifying the genetic diversity among genotypes of inbred guava families associated with the use of the Ward-Modified Location Model method.


Assuntos
Psidium/genética , Computadores , Frutas/química , Variação Genética , Genótipo , Processamento de Imagem Assistida por Computador/métodos , Fenótipo , Melhoramento Vegetal/métodos , Psidium/química , Sementes/química , Sementes/genética
13.
Genet Mol Res ; 14(4): 15376-89, 2015 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-26634503

RESUMO

The Ward-MLM procedure was used to evaluate genetic variation in four backcross progenies and in their parents, hybrid F1 HD13 and donor parent Passiflora sublanceolata. Sixteen quantitative descriptors and five qualitative characteristics of relevance to ornamental flower production were assessed. Using the pseudo-F and pseudo-T² criteria, we identified four groups among these plants in two evaluation periods. In both evaluations, the BC1 plants showed greater dissimilarity to their recurrent parent, but showed high genetic similarity with the P. sublanceolata parent. The first two canonical variables produced by the Ward-MLM procedure accounted for over 90% of the variation in both evaluation periods, enabling the representation of diversity through two-dimensional graphics. Groups II and IV were formed in the first assessment period. Groups I and IV formed in the second period and showed plants with selection potential. We found that it was essential to use both qualitative and quantitative variables for this analysis. Assessments of quantitative descriptors indicate that the selection of BC1 plants can be performed in any of the four progenies. Because of the similarities observed for some floral descriptors between BC1 and the P. sublanceolata parent, a second generation backcross was not recommended. However, the selection of BC1 plants for evaluation and direct use as an ornamental cultivar, or as a resource in other breeding programs, can be recommended.


Assuntos
Passiflora/genética , Passifloraceae/genética , Seleção Artificial/genética , Cruzamento/métodos , Cruzamentos Genéticos , Flores/genética , Variação Genética/genética , Genótipo , Análise Multivariada
14.
Genet Mol Res ; 13(4): 9828-45, 2014 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-25501192

RESUMO

Morphological characterization is the most accessible and used method to quantify the genetic diversity of the available germplasm. The multivariate statistical method is highly important for this purpose. This study aimed to characterize parents and hybrids of Passiflora according to morphoagronomic descriptors and estimate the genetic divergence between them based on the joint analysis of qualitative and quantitative variables using the Ward-modified location model (MLM) procedure. One hundred and thirty-eight individuals were assessed (10 P. edulis, 10 P. setacea, and 118 interspecific hybrids) using 23 quantitative and 12 qualitative descriptors. The values for the quantitative descriptors were measured and subjected to multivariate statistics using the Ward-MLM strategy. Large genetic variability was detected by the morphoagronomic data in the 138 genotypes that were evaluated, and the hybrids presented higher variability than the parents. Pseudo-F and pseudo-t2 criteria showed that the optimal number of groups was three. Group I was composed of 118 hybrid genotypes; group II was composed of the 10 P. setacea genotypes, and group III was composed of the 10 P. edulis genotypes. The longest distance was found between groups II and III (474.96). The shortest distance was detected between groups I and II (198.78), which indicates that the segregating population is genetically closer to P. setacea than to P. edulis. The Ward-MLM procedure is a useful tool to detect genetic diversity and group accessions using both qualitative and quantitative variables.


Assuntos
Hibridização Genética , Passiflora/anatomia & histologia , Passiflora/genética , Cruzamentos Genéticos , Flores/anatomia & histologia , Flores/genética , Frutas/anatomia & histologia , Frutas/genética , Genótipo , Funções Verossimilhança , Análise Multivariada , Fenótipo , Característica Quantitativa Herdável , Especificidade da Espécie
15.
Arq. bras. med. vet. zootec ; 66(6): 1623-1630, 12/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-735793

RESUMO

We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed in 5 L Styrofoam boxes, stored for 4h, 12h, 24h, and 36h, and then analyzed. For viability analysis a pair of ovaries from two additional females was divided into nine fragments; one fragment was immediately destined for viability analysis (Trypan blue test) and the other fragments were stored as previously described, until 24h and then analyzed. After 4h storage in ACP(r) medium, the follicular integrity was similar to control (87.8% vs 94.4%, respectively); however, ultrastructural analyses revealed swollen mitochondria as the first signals of PF degeneration. It was observed that ACP(r) (66.7%) was more efficient than PBS (49.4%) to preserve the morphological integrity after 36h storage (P<0.05); however, no differences were observed on follicular viability (P>0.05). In conclusion, the use of the ACP(r) is recommended for the short-term preservation of Pecari tajacu preantral follicles...


Compararam-se protocolos para a preservação por curtos períodos de folículos ovarianos pré-antrais (PFs) de catetos, utilizando meios à base de solução salina tamponada (PBS) ou água de coco em pó (ACP(r)). Para a análise morfológica, cada par de ovários coletados de seis fêmeas foi dividido em nove fragmentos. Um fragmento foi destinado para a análise da morfologia (histologia e microscopia eletrônica de transmissão - MET), constituindo o grupo controle, e os demais fragmentos foram colocados em tubos contendo PBS ou ACP(r), acondicionados em caixas térmicas de poliestireno expandido de 5L, armazenados durante quatro, 12, 24 e 36 horas, e, então, analisados. Para a análise da viabilidade, pares de ovários de duas fêmeas adicionais foram divididos em nove fragmentos; um deles foi imediatamente destinado à análise da viabilidade (teste com azul de Trypan), os outros fragmentos foram armazenados como descrito previamente até 24h e, então, foram analisados. Após quatro horas de armazenamento em meio ACP(r), a integridade folicular foi similar ao grupo controle (87,8% vs. 94,4%, respectivamente); contudo, a análise ultraestrutural revelou mitocôndrias edemaciadas como os primeiros sinais de degeneração dos PFs. Foi observado que o ACP(r) (66,7%) foi mais eficiente do que o PBS (49.4%) em preservar a integridade morfológica após 36h (p<0,05); entretanto, nenhuma diferença foi observada para a viabilidade folicular (P>0,05). Em conclusão, o uso da ACP(r) é recomendado para a preservação por curtos períodos de folículos pré-antrais de Pecari tajacu...


Assuntos
Animais , Folículo Ovariano , Ovário , Preservação da Fertilidade/instrumentação , Suínos , Protocolos Clínicos , Preservação da Fertilidade/veterinária
16.
Reproduction ; 147(6): 753-64, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24516176

RESUMO

This study was conducted to characterize the major proteins of the peccary seminal plasma, based on the semen samples collected from nine adult and reproductively sound animals. Our approach included the use of two-dimensional electrophoresis followed by Coomassie blue staining and analysis of polypeptide maps with PDQuest Software (Bio-Rad). Proteins were identified by tandem mass spectrometry (LC-MS/MS). We detected 179 protein spots per gel and 98 spots were identified by mass spectrometry, corresponding to 23 different proteins. The combined intensity of those spots accounted for 56.2±6% of the intensities of all spots and 60.9% of the intensities of spots presented in every protein map. Protein spots identified as clusterin represented 19.7±8.3% of the integrated optical densities of all spots detected in the seminal plasma maps. There was a negative association (r=-0.87; P<0.05) between the intensity of a clusterin spot and the percentage of sperm with functional membrane. Spermadhesin porcine seminal plasma protein 1 and bodhesin 2 comprised 5.4±1.9 and 8.8±3.9% of the total intensity of all spots respectively. Many proteins appeared in a polymorphic pattern, such as clusterin (27 spots), epididymal secretory glutathione peroxidase (ten spots), inter-α-trypsin inhibitor (12 spots), and IgG-binding protein (ten spots), among others. In conclusion, we presently describe the major seminal plasma proteome of the peccary, which exhibits a distinct high expression of clusterin isoforms. Knowledge of wild species reproductive biology is crucial for an understanding of their survival strategies and adaptation in a changing environment.


Assuntos
Artiodáctilos/metabolismo , Sêmen/química , Proteínas de Plasma Seminal/análise , alfa-Globulinas/análise , Animais , Cromatografia Líquida , Clusterina/análise , Conservação dos Recursos Naturais , Eletroforese em Gel Bidimensional , Glutationa Peroxidase/análise , Linfocinas/análise , Masculino , Mapas de Interação de Proteínas , Isoformas de Proteínas , Proteômica/métodos , Espectrometria de Massas em Tandem
17.
Arq. bras. med. vet. zootec ; 65(4): 1257-1260, Aug. 2013. graf, tab
Artigo em Inglês | LILACS | ID: lil-684488

RESUMO

Soluções hiposmóticas com diferentes concentrações (0, 50, 100, 150, 200mOsm/L) foram testadas para a avaliação funcional da membrana espermática de catetos (n=13). Foi verificado que o número de espermatozoides reagidos diminuía (P<0,05) de acordo com o aumento da osmolaridade do meio. A maior porcentagem (71,8%) de espermatozoides reagidos, bem como a menor variação nas respostas osmóticas, foi detectada com o uso de água destilada (0mOsm/L) (P<0,05), a qual também apresentou a menor variação nos resultados, de acordo com os erros padrão verificados. Em conclusão, a água destilada aparenta ser uma solução adequada para o uso no teste hiposmótico para sêmen de catetos.


Assuntos
Animais , Andrologia , Análise do Sêmen/métodos , Animais Selvagens/classificação , Suínos/classificação
18.
Anim Reprod Sci ; 140(3-4): 279-85, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23820069

RESUMO

We analyzed the sperm characteristics of captive six-banded armadillos (Euphractus sexcinctus), by the assessment of sperm morphology, morphometry, and ultrastructure. In general, armadillo's ejaculates present more than 80% of sperm within the range considered normal for sperm morphology currently accepted for other mammals. Coiled tails (3.9%) and detached heads (2.8%) were the defects most frequently verified. The morphometric analysis revealed that the total length of six-banded armadillo sperm is 77.6±1.2µm, and the length of the tail is 64.7±1.1µm on average. They also present a big head that corresponds to 16.6% of the entire sperm. Through transmission electron microscopy, we identified the presence of electron lucent points into the nucleus and the presence of about 45 mitochondria spirals in the mitochondrial sheath midpiece as a peculiarity of the six-banded armadillo sperm.


Assuntos
Tatus/anatomia & histologia , Espermatozoides/ultraestrutura , Animais , Núcleo Celular/ultraestrutura , Masculino , Microscopia Eletrônica de Transmissão/veterinária , Análise do Sêmen/veterinária
19.
Anat Histol Embryol ; 42(4): 304-11, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23278244

RESUMO

The aim of this research was to characterize the preantral ovarian follicular population in collared peccaries (Tayassu tajacu) using light and electron microscopy. Ovaries from six mature females were collected and further fixed for histological and ultrastructural analysis. A total of 33273.45 ± 5789.99 preantral follicles (PFs) were estimated for the population in each ovary. Most preantral follicles were primordial (91.56%), followed by primary (6.29%) and secondary (2.15%) ones. Most PFs were morphologically normal (94.4%), and only a few were atretic (5.6%). At histology assessment, amounts of lipid droplets were observed into the oocyte cytoplasm, which was confirmed through ultrastructural analysis. This work characterizes for the first time the ovarian population of preantral follicles, total and per category, in collared peccaries (Tayassu tajacu). The general follicles featured at primordial, primary and secondary categories are very similar to those described for other species.


Assuntos
Artiodáctilos/fisiologia , Folículo Ovariano/anatomia & histologia , Folículo Ovariano/fisiologia , Animais , Feminino
20.
Artigo em Inglês | MEDLINE | ID: mdl-22316345

RESUMO

A total of 230 samples of processed rice and its sub-products or derived products were analysed to establish the co-occurrence of several mycotoxins. Samples were analysed in the period 2007-2009 due to the outbreak of beriberi associated with the consumption of rice stored in inappropriate conditions in Brazil. According to data from the Ministry of Health, 323 cases of disease were registered in 2006, of which at least 47 cases resulted in death. The occurrence of total aflatoxin (AFT) (aflatoxin B(1) + B(2) + G(1) + G(2)), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON), and citreoviridin (CTV) was 58.7%, 40.0%, 45.2%, 8.3% and 22.5%, respectively. From 166 rice samples analysed, 55% had levels <0.11 µg kg(-1) for AFT. For OTA and ZON, of 165 rice samples analysed, 28% and 29% were contaminated with levels from 0.20 to 0.24 µg kg(-1) and from 3.6 to 290.0 µg kg(-1), respectively. One sample (0.6%) was contaminated with 4872.0 µg kg(-1) of ZON. A total of 91% of rice samples (n = 165) did not contain detectable DON (<30.00 µg kg(-1)), although the highest level of contamination was found to be 244 µg kg(-1). From the total of 65 samples analysed, 94% had no detectable CTV (<0.9 µg kg(-1)), with a range from 0.9 to 31.1 µg kg(-1) in 6% of the samples. The highest levels of contamination were found in rice sub-products or derived products from the husk and rice bran. Co-occurrence was observed for AFT and ZON in 17.0%, AFT and OTA in 24.2%, AFT and CTV in 6.2%, OTA and CTV in 4.6%, and ZON and CTV in 3.1%. These fractions were also the major contributors for the co-occurrence. The results found show the necessity of monitoring rice production.


Assuntos
Contaminação de Alimentos/análise , Micotoxinas/análise , Oryza/química , Aflatoxina B1/análise , Aflatoxinas/análise , Aurovertinas/análise , Brasil , Análise de Alimentos/métodos , Análise de Alimentos/normas , Micotoxinas/normas , Ocratoxinas/análise , Padrões de Referência , Tricotecenos/análise , Zearalenona/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...