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1.
Rev Port Cir Cardiotorac Vasc ; 27(1): 43-45, 2020.
Artigo em Português | MEDLINE | ID: mdl-32239825

RESUMO

The authors present the case of a previously healthy, 22-year-old male nonsmoker who sought emergency room treatment complaining of retrosternal pain. He reported a history of odynophagia two days before, followed by produc- tive cough, fever and dyspnea. On chest radiography, a line could be observed surrounding the heart and the continuous diaphragm sign. The chest computed tomography scan confirmed the presence of pneumomediastinum and soft tissue emphysema. The case was discussed in a multidisciplinary team, and the possibility of surgical intervention was rejected. Conservative treatment was decided with complete resolution of the pneumomediastinum.

2.
Biol Trace Elem Res ; 2020 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-32266641

RESUMO

Ruthenium(II)/benzonitrile complexes have demonstrated promising anticancer properties. Considering that there are no specific therapies for treating sarcoma, we decided to evaluate the cytotoxic, genotoxic, and lethal effects of cis-[RuCl(BzCN)(phen)(dppb)]PF6 (BzCN = benzonitrile; phen = 1,10-phenanthroline; dppb = 1,4-bis-(diphenylphosphino)butane), as well as the mechanism of cell death induction that occurs against murine sarcoma-180 tumor. Thus, MTT assay was applied to assess the ruthenium cytotoxicity, showing that the compound is a more potent inhibitor for the sarcoma-180 tumor cell viability than normal cells (lymphocytes). The comet assay indicated low genotoxic for normal cells. cis-[RuCl(BzCN)(phen)(dppb)]PF6 also showed moderate lethality in Artemia salina. The complex induced cell cycle arrest in the G0/G1 phase in sarcoma-180 cells. In addition, the complex caused S180 cells to die by apoptosis by an increase in Annexin-V-positive cells and morphological changes typical of apoptotic cells. Additionally, cis-[RuCl(BzCN)(phen)(dppb)]PF6 increased the gene expression of Bax, Casp3, and Tp53 in S180 cells. By using a western blot, we observed an increased protein level of TNF-R2, Bax, and p21. In conclusion, cis-[RuCl(BzCN)(phen)(dppb)]PF6 is active and selective for sarcoma-180 cells, leading to cell cycle arrest at the G0/G1 and cell death through a caspases-mediated and Tp53/p21-mediated pathway.

3.
Int J Mol Sci ; 21(5)2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32155742

RESUMO

Acacia dealbata biomass, either from forest exploitation or from the management of invasive species, can be a strategic topic, namely as a source of high-value compounds. In this sense, the present study aimed at the detailed characterization of the lipophilic components of different morphological parts of A. dealbata and the evaluation of their cytotoxicity in cells representative of different mammals' tissues. The chemical composition of lipophilic extracts from A. dealbata bark, wood and leaves was evaluated using gas chromatography-mass spectrometry (GC-MS). Terpenic compounds (representing 50.2%-68.4% of the total bark and leaves extracts, respectively) and sterols (60.5% of the total wood extract) were the main components of these extracts. Other constituents, such as fatty acids, long-chain aliphatic alcohols, monoglycerides, and aromatic compounds were also detected in the studied extracts. All the extracts showed low or no cytotoxicity in the different cells tested, demonstrating their safety profile and highlighting their potential to be used in nutraceutical or pharmaceutical applications. This study is therefore an important contribution to the valorization of A. dealbata, demonstrating the potential of this species as a source of high value lipophilic compounds.

4.
Carbohydr Polym ; 230: 115604, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31887959

RESUMO

Conductive natural-based separators for application in polymer electrolyte fuel cells (PEFCs) were fabricated by combining a bacterial polysaccharide, i.e. bacterial cellulose (BC), and an algae sulphated polysaccharide, i.e. fucoidan (Fuc). The diffusion of fucoidan aqueous solution containing a natural-based cross-linker, viz. tannic acid, into the wet BC nanofibrous three-dimensional network, followed by thermal cross-linking, originated fully bio-based proton exchange membranes (PEMs). The PEMs present thermal-oxidative stability in the range of 180-200 °C and good dynamic mechanical performance (storage modulus ≥ 460 MPa). Additionally, the BC/Fuc membranes exhibit protonic conductivity that increases with increasing relative humidity (RH), which is a typical feature for numerous water-mediated proton conductors. The traditional Arrhenius-type plots demonstrate a linear behaviour with a maximum protonic conductivity of 1.6 mS cm-1 at 94 °C and 98 % RH. The results showed that these fully bio-based conductive membranes have potential as eco-friendly alternatives to other PEMs for application in PEFCs.

5.
Mol Pharm ; 17(3): 837-851, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-31977228

RESUMO

Delivery efficiencies of theranostic nanoparticles (NPs) based on passive tumor targeting strongly depend either on their blood circulation time or on appropriate modulations of the tumor microenvironment. Therefore, predicting the NP delivery efficiency before and after a tumor microenvironment modulation is highly desirable. Here, we present a new erythrocyte membrane-camouflaged magnetofluorescent nanocarrier (MMFn) with long blood circulation time (92 h) and high delivery efficiency (10% ID for Ehrlich murine tumor model). MMFns owe their magnetic and fluorescent properties to the incorporation of manganese ferrite nanoparticles (MnFe2O4 NPs) and IR-780 (a lipophilic indocyanine fluorescent dye), respectively, to their erythrocyte membrane-derived camouflage. MMFn composition, morphology, and size, as well as optical absorption, zeta potential, and fluorescent, magnetic, and magnetothermal properties, are thoroughly examined in vitro. We then present an analytical pharmacokinetic (PK) model capable of predicting the delivery efficiency (DE) and the time of peak tumor uptake (tmax), as well as changes in DE and tmax due to modulations of the tumor microenvironment, for potentially any nanocarrier. Experimental PK data sets (blood and tumor amounts of MMFns) are simultaneously fit to the model equations using the PK modeling software Monolix. We then validate our model analytical solutions with the numerical solutions provided by Monolix. We also demonstrate how our a priori nonmechanistic model for passive targeting relates to a previously reported mechanistic model for active targeting. All in vivo PK studies, as well as in vivo and ex vivo biodistribution studies, were conducted using two noninvasive techniques, namely, fluorescence molecular tomography (FMT) and alternating current biosusceptometry (ACB). Finally, histopathology corroborates our PK and biodistribution results.

6.
Int J Mol Sci ; 21(2)2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31963392

RESUMO

The Warburg effect is an emerging hallmark of cancer, which has the tumor suppressor p53 as its major regulator. Herein, we unveiled that p53 activation by (S)-tryptophanol-derived oxazoloisoindolinone (SLMP53-1) mediated the reprograming of glucose metabolism in cancer cells and xenograft human tumor tissue, interfering with angiogenesis and migration. Particularly, we showed that SLMP53-1 regulated glycolysis by downregulating glucose transporter 1 (GLUT1), hexokinase-2 (HK2), and phosphofructokinase-2 isoform 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-3 (PFKFB3) (key glycolytic enzymes), while upregulating the mitochondrial markers synthesis of cytochrome c oxidase 2 (SCO2), cytochrome c oxidase subunit 4 (COX4), and OXPHOS mitochondrial complexes. SLMP53-1 also downregulated the monocarboxylate transporter 4 (MCT4), causing the subsequent reduction of lactate export by cancer cells. Besides the acidification of the extracellular environment, SLMP53-1 further increased E-cadherin and reduced metalloproteinase-9 (MMP-9) expression levels in both cancer cells and xenograft human tumor tissue, which suggested the interference of SLMP53-1 in extracellular matrix remodeling and epithelial-to-mesenchymal transition. Consistently, SLMP53-1 depleted angiogenesis, decreasing endothelial cell tube formation and vascular endothelial growth factor (VEGF) expression levels. SLMP53-1 also exhibited synergistic growth inhibitory activity in combination with the metabolic modulator dichloroacetic acid. These data reinforce the promising application of the p53-activating agent SLMP53-1 in cancer therapy, by targeting p53-mediated pathways of growth and dissemination.

7.
Antioxidants (Basel) ; 8(12)2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31801290

RESUMO

Salix spp. have been exploited for energy generation, along with folk medicine use of bark extracts for antipyretic and analgesic benefits. Bark phenolic components, rather than salicin, have demonstrated interesting bioactivities, which may ensure the sustainable bioprospection of Salix bark. Therefore, this study highlights the detailed phenolic characterization, as well as the in vitro antioxidant, anti-hypertensive, Staphylococcus aureus growth inhibitory effects, and biocompatibility of Salix atrocinerea Brot., Salix fragilis L., and Salix viminalis L. bark polar extracts. Fifteen phenolic compounds were characterized by ultra-high-performance liquid chromatography-ultraviolet detection-mass spectrometry analysis, from which two flavan-3-ols, an acetophenone, five flavanones, and a flavonol were detected, for the first time, as their bark components. Salix bark extracts demonstrated strong free radical scavenging activity (5.58-23.62 µg mL-1 IC50 range), effective inhibition on angiotensin-I converting enzyme (58-84%), and S. aureus bactericidal action at 1250-2500 µg mL-1 (6-8 log CFU mL-1 reduction range). All tested Salix bark extracts did not show cytotoxic potential against Caco-2 cells, as well as S. atrocinerea Brot. and S. fragilis L. extracts at 625 and 1250 µg mL-1 against HaCaT and L929 cells. These valuable findings can pave innovative and safer food, nutraceutical, and/or cosmetic applications of Salix bark phenolic-containing fractions.

8.
Biomolecules ; 9(12)2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31835386

RESUMO

The current interest of the scientific community for the exploitation of high-value compounds from macroalgae is related to the increasing knowledge of their biological activities and health benefits. Macroalgae phenolic compounds, particularly phlorotannins, have gained particular attention due to their specific bioactivities, including antioxidant, antiproliferative, or antidiabetic. Notwithstanding, the characterization of macroalgae phenolic compounds is a multi-step task, with high challenges associated with their isolation and characterization, due to the highly complex and polysaccharide-rich matrix of macroalgae. Therefore, this fraction is far from being fully explored. In fact, a critical revision of the extraction and characterization methodologies already used in the analysis of phenolic compounds from macroalgae is lacking in the literature, and it is of uttermost importance to compile validated methodologies and discourage misleading practices. The aim of this review is to discuss the state-of-the-art of phenolic compounds already identified in green, red, and brown macroalgae, reviewing their structural classification, as well as critically discussing extraction methodologies, chromatographic separation techniques, and the analytical strategies for their characterization, including information about structural identification techniques and key spectroscopic profiles. For the first time, mass spectrometry data of phlorotannins, a chemical family quite exclusive of macroalgae, is compiled and discussed.

9.
Mar Drugs ; 17(10)2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31569367

RESUMO

Marine resources are considered as a very promising source of bioactive molecules, and macroalgae in particular have gained special attention, due to their structurally diverse composition. Particular interest has been devoted to the brown macroalga Bifurcaria bifurcata, due to their abundance in bioactive linear diterpenes. In this appraisal, a thorough review concerning the methodologies used in the extraction, fractionation, and identification of diterpenes from B. bifurcata is provided and discussed in detail. An exhaustive compilation of the mass spectra and nuclear magnetic resonance (NMR) data are also provided. The in vitro and in chemico assays already performed to assess different biological activities attributed to B. bifurcata diterpenes are also reviewed, emphasizing the use of isolated components, enriched fractions, or crude extracts. The associated major strengths and challenges for the exploitation of B. bifurcata diterpenes for high-value applications are critically discussed.

10.
Int J Mol Sci ; 20(17)2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31480214

RESUMO

Substantial amounts of vine wastes are produced during vineyard management, and the chemical profiling of high-value lipophilic phytochemicals is becoming crucial in order to find a complementary route towards their integrated valorisation. The prospection of bioactive phytochemicals from unripe grape, vine shoot, vine cane, stalk and leaf dichloromethane extracts was carried out by gas chromatography-mass spectrometry (GC-MS), analysing samples from a mixture of four red Vitis vinifera L. varieties (Baga, Aragonez, Água Santa and Shiraz), collected at Bairrada Appellation, as a representative case study of typical multi-variety Portuguese vineyards. Vine wastes showed distinct amounts of lipophilic extract, ranging from 0.68% (vine canes) to 13.35% (vine leaves) at dry weight (dw). Thirty-three components were identified, including fatty acids and alcohols, sterols and triterpenoids accounting for amounts from 118.9 mg/100 g dw to 1512.0 mg/100 g dw. The integrated study revealed that unripe grape, stalk and leaf dichloromethane extracts stood out as possible sources of triterpenic compounds (103.2 to 653.5 mg/100 g dw), with lupeol, ursolic and oleanolic acids prevailing. Leaf extract is also reported as an undervalued source of α-tocopherol, as the major component detected in this matrix (300.5 mg/100 g dw). These exploratory results are a relevant contribution for the exploitation of undervalued vine residues as a source of health-promoting components with the potential to be used as supplements or nutraceutical ingredients.


Assuntos
Lipídeos/química , Compostos Fitoquímicos/farmacologia , Vitis/química , Resíduos/análise , Álcoois/análise , Ácidos Graxos/análise , Extratos Vegetais/química , Esteróis/análise , Triterpenos/análise
11.
J Proteome Res ; 18(8): 3174-3183, 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31290314

RESUMO

Obesity is a public health problem and a risk factor for pathologies such type 2 diabetes mellitus, cardiovascular diseases, and nonalcoholic fatty liver disease. Given these clinical implications, there is a growing interest to understand the pathophysiological mechanism of obesity. Changes in lipid metabolism have been associated with obesity and obesity-related complications. However, changes in the lipid profile of obese children have been overlooked. In the present work, we analyzed the serum phospholipidome of overweight and obese children by HILIC-MS/MS and GC-MS. Using this approach, we have identified 165 lipid species belonging to the classes PC, PE, PS, PG, PI, LPC, and SM. The phospholipidome of overweight (OW) and obese (OB) children was significantly different from normal-weight children (control). Main differences were observed in the PI class that was less abundant in OW and OB children and some PS, PE, SM, and PC lipid species are upregulated in obese and overweight children. Although further studies are needed to clarify some association between phospholipid alterations and metabolic changes, our results highlight the alteration that occurs in the serum phospholipid profile in obesity in children.

12.
Biodivers Data J ; 7: e34327, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31182911

RESUMO

Background: The data presented here come from field observations of Aves between August 2013 and October 2018 as part of a LIFE research project aiming to preserve and restore three coastal wetlands from Praia da Vitória (Terceira Island, Azores, Portugal). Systematic monthly observations were carried out for five years in order to provide a checklist and monitoring of bird species and subspecies observed in three sites: Paul da Praia da Vitória (PPV), Paul do Belo Jardim (PBJ) and Paul da Pedreira do Cabo da Praia (PPCP). Main objectives were to determine their ornithological richness while also adding data to the overall knowledge of Azorean Avifauna and to monitor seasonal and between-year variation on species abundance. New information: During a five-year observation period (2013-2018), a total of 82,985 birds belonging to 108 species/subspecies were observed. From this, 16,663 were in PPV, 11,793 from PBJ and 54,529 from PPCP. The total richness was 55, 40 and 85, respectively. Three species are first records for the Azores: Aythya americana (Eyton, 1838); Chlidonias leucopterus (Temminck, 1815) and Tringa brevipes (Vieillot, 1816). One species is a new record for Terceira Island: Lophodytes cucullatus (Linnaeus, 1758).

13.
Odontology ; 107(4): 530-535, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31030294

RESUMO

The aim of this study was to evaluate self-reported periodontitis (PD) prevalence in migraineurs as well as to investigate the association between both diseases. A cross-sectional survey was carried out including patients diagnosed with migraine attending 12 Spanish Headache Units. We determined diagnosis of PD administering a validated self-reported questionnaire. Socio-demographic, clinical and medical information, comorbidities, daily habits, migraine characteristics and medication were collected using a questionnaire. Of the 651 consecutive migraineurs included in the study, 393 suffered from chronic migraine (CM). Self-reported PD was detected in 327 patients with migraine (50.2%). Migraineurs with self-reported PD were significantly older and had a previous history of fibromyalgia, stress, anxiety, depression, and allodynia (all P < 0.001). Additionally, this group of patients consumed more topiramate (P = 0.008) and simple analgesics (P < 0.001) than patients with migraine and without self-reported PD. Also, they were less active physically and belonged to a low education level (both P < 0.001). Prevalence of self-reported PD was significantly higher in chronic migraineurs compared to those diagnosed with episodic migraine (EM) (53.9% vs. 44.6%, P = 0.019). Logistic regression analyses showed that self-reported PD was associated with CM (OR 1.456; 95% CI 1.062-1.997, P = 0.020). However, after adjusting for significant confounders, the association was attenuated (OR 1.100; 95% CI 0.784-1.543, P = 0.581). We concluded that self-reported PD was significantly more frequent in CM compared to EM. Self-reported PD was associated with the presence of CM, although some comorbidities shared by both diseases could have an effect on this association.


Assuntos
Transtornos de Enxaqueca , Periodontite , Estudos Transversais , Humanos , Autorrelato , Espanha , Inquéritos e Questionários
14.
Int J Mol Sci ; 20(4)2019 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-30795544

RESUMO

Worldwide, foodborne diseases are a growing public health problem. Among the infectious bacteria, non-typhoidal Salmonella enterica serovars (NTS) are the major cause of hospitalization and death, and the emergence and spread of their antibiotic-resistance is becoming a worldwide health issue. This, coupled with the restrictions of antibiotics use in agriculture and animal production, calls for alternative approaches to solve this problem. Plant-derived aqueous extracts compounds could provide novel straightforward approaches to control pathogenic bacteria. This review discusses the antimicrobial activity of aqueous plant extracts against Salmonella serovars, the possible mechanisms of action involved, which components/structures might be responsible for such activity, and the current challenges for the use of these extracts/components in Salmonella infection management and their application perspectives.


Assuntos
Antibacterianos/uso terapêutico , Extratos Vegetais/uso terapêutico , Infecções por Salmonella/tratamento farmacológico , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Humanos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Salmonella enterica/efeitos dos fármacos
15.
Int J Mol Sci ; 20(1)2019 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-30625994

RESUMO

Three different high performance liquid chromatography columns were accessed for phenolic compounds (PC) separation in the hydrophilic fraction of extra virgin olive oil (EVOO). Two fully porous C18 bonded silica phases and one partially porous biphenyl column were used. Biphenyl column allowed for an increase of more than 30% in peak capacity (nc), higher selectivity (α) (1.045), and improved retention (k), with a reduction of 22.1% in the retention time. The higher resolution (Rs) was obtained by using the biphenyl column, with a fair separation of oleuropein aglycone isomers (OAI) and a good identification of caffeic acid (CA). Tyrosol (T), hydroxytyrosol (HT), and dihydroxyphenyl glycol (DHPG) were also well separated and identified. Moreover, the method using a biphenyl column was fully validated according to the requirements for new methods. For all parameters, the method applying the biphenyl column proved to be a reliable, accurate, and robust tool for separation, identification, and quantification of the main PCs in EVOOs.


Assuntos
Compostos de Bifenilo/química , Cromatografia Líquida de Alta Pressão/métodos , Azeite de Oliva/química , Fenóis/isolamento & purificação , Interações Hidrofóbicas e Hidrofílicas , Limite de Detecção , Espectrometria de Massas , Fenóis/química , Álcool Feniletílico/análogos & derivados , Álcool Feniletílico/análise
16.
Cien Saude Colet ; 24(1): 77-86, 2019 Jan.
Artigo em Português | MEDLINE | ID: mdl-30698242

RESUMO

The fear of falling constitutes a real risk factor for falls. It has also been associated with functional decline, decreased quality of life and increased social isolation among the elderly population. This study analyzed predictors of the fear of falling in elderly Portuguese people that live in the community. A cross-sectional study with a convenience sample of 98 participants (57.1% women; mean age 74.07 ± 8.74 years) was conducted. Data were collected with a protocol which includes a questionnaire for sociodemographic and health information, the Activity Specific Balance Confidence Scale, the Hospital Anxiety and Depression Scale, the Lubben Social Network Scale-6, the Timed Up and Go, and Five Times Sit to Stand Test. Data were analyzed using descriptive and inferential statistics. The results showed that female gender (p = 0.01), the perception of good (p = 0.01) and moderate (p = 0.02) physical health and the symptoms of depression (p ≈ 0.00) are predictors of fear of falling. Controlling these predictors is a fundamental aspect for promoting the independence of elderly people minimizing the consequences that are associated with the fear of falling.


Assuntos
Acidentes por Quedas/estatística & dados numéricos , Depressão/epidemiologia , Medo/psicologia , Qualidade de Vida , Idoso , Idoso de 80 Anos ou mais , Estudos Transversais , Feminino , Nível de Saúde , Humanos , Vida Independente/estatística & dados numéricos , Masculino , Portugal/epidemiologia , Equilíbrio Postural , Fatores de Risco , Fatores Sexuais , Isolamento Social/psicologia , Inquéritos e Questionários
17.
Food Res Int ; 115: 167-176, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30599929

RESUMO

This study aimed to assess the effect of high pressure (300 and 600 MPa) and enzymatic extraction (pectinase and cellulase) on the phenolic compounds profile, antioxidant capacity and antimicrobial activity of extracts from pomegranate by-products. Antimicrobial activity against eight different strains of pathogenic and contaminant bacteria and against five beneficial bacteria including Lactobacillus and Bifidobacterium strains were determined. The maximum level of total phenolic content, as well as antioxidant capacity were observed at 300 MPa, however enzymatic extraction did not improve the extraction yields. Punicalagin isomers and bis-hexahydroxydiphenoyl-glucoside isomer were the most abundant phenolic compounds found in the extracts. All pomegranate peel extracts demonstrated selective antimicrobial activity against all pathogenic bacteria without affecting beneficial ones. Pressurized extracts presented lower minimum inhibitory concentration against Bacillus cereus and Pseudomonas aeruginosa and lower minimum bactericidal concentration against B. cereus, while, enzymatic extracts presented lower minimum bactericidal concentration for Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus and Listeria monocytogenes. Principal component analyses reveled that antioxidant activity and phenolic compounds content were strongly related with antimicrobial activity. Pomegranate peels extracts obtained by high pressure extraction could so be used as a source of high added-value bioactive compounds for antioxidant and antimicrobial applications.


Assuntos
Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Lythraceae/química , Extratos Vegetais/farmacologia , Antibacterianos/isolamento & purificação , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Antioxidantes/análise , Bactérias/efeitos dos fármacos , Depuradores de Radicais Livres , Frutas/química , Taninos Hidrolisáveis , Testes de Sensibilidade Microbiana , Fenóis/análise , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Pressão
18.
J Hazard Mater ; 370: 13-23, 2019 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-30503313

RESUMO

Porphyrins are known as effective photosensitizers and can be an interesting key in phototreatment of water contaminated with micropollutants such as pharmaceuticals. They already showed to be efficient photocatalysts for the degradation of dyes, chlorophenols and other pollutants. This work demonstrates the applicability of 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin (H2TF5PP) as photosensitizer for treatment of water contaminated with metoprolol, a highly prescribed ß-blocker, which is not completely removed in sewage treatment plants. Studies were firstly developed under homogeneous conditions with simulated solar radiation and porphyrin was found to be efficient in the photodegradation of metoprolol, following a pseudo-first order kinetics with ca. 90% metoprolol degradation after 12 h. Experiments in presence of scavengers confirmed the mechanism of degradation via singlet oxygen. Appearance of several new peaks in HPLC chromatograms indicates the formation of products, identified by HPLC-MSn. Furthermore, the porphyrin was immobilized on a silica support and used as heterogeneous photocatalyst in degradation of metoprolol. Experiments using this heterogeneous photocatalyst under real solar irradiation were also performed, and similar results were obtained. Kinetic comparison of metoprolol photodegradation in buffer solution and in real wastewater treatment plant effluent showed that the efficiency of the immobilized porphyrin was not decreased by the complex matrix of the effluent.


Assuntos
Antagonistas de Receptores Adrenérgicos beta 1/química , Metoprolol/química , Fármacos Fotossensibilizantes/efeitos da radiação , Porfirinas/efeitos da radiação , Luz Solar , Poluentes Químicos da Água/química , Fotólise , Fármacos Fotossensibilizantes/química , Porfirinas/química , Oxigênio Singlete/química , Purificação da Água/métodos
19.
Carbohydr Polym ; 206: 187-197, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30553312

RESUMO

The utilization of natural compounds, such as phenolic acids and biopolymers, in the healthcare domain is gaining increasing attention. In this study, bacterial nanocellulose (BC) membranes were loaded with ionic liquids (ILs) based on phenolic acids. These ionic compounds, with improved solubility and bioavailability, were prepared by combining the cholinium cation with anions derived from caffeic, ellagic and gallic acids. The obtained BC-ILs membranes were homogeneous, conformable and their swelling ability agreed with the solubility of each IL. These membranes revealed a controlled ILs dissolution rate in the wet state and high antioxidant activity. In vitro assays performed with Raw 264.7 macrophages and HaCaT keratinocytes revealed that these novel BC-ILs membranes are non-cytotoxic and present relevant anti-inflammatory properties. Diffusion studies with Hanson vertical diffusion cells showed a prolonged release profile of the ILs from the BC membranes. Thus, this work, successfully demonstrates the potential of BC-ILs membranes for skin treatment.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Celulose/química , Líquidos Iônicos/farmacologia , Administração Cutânea , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/química , Antioxidantes/administração & dosagem , Antioxidantes/química , Ácidos Cafeicos/administração & dosagem , Ácidos Cafeicos/química , Ácidos Cafeicos/farmacologia , Linhagem Celular , Colina/administração & dosagem , Colina/química , Colina/farmacologia , Liberação Controlada de Fármacos , Módulo de Elasticidade , Ácido Elágico/administração & dosagem , Ácido Elágico/química , Ácido Elágico/farmacologia , Feminino , Ácido Gálico/administração & dosagem , Ácido Gálico/química , Ácido Gálico/farmacologia , Humanos , Líquidos Iônicos/administração & dosagem , Líquidos Iônicos/química , Membranas Artificiais , Camundongos , Nanoestruturas/química , Pele/efeitos dos fármacos
20.
Ciênc. Saúde Colet ; 24(1): 77-86, ene. 2019. tab
Artigo em Português | LILACS-Express | ID: biblio-974813

RESUMO

Resumo O medo de cair é um fator de risco de queda. Também tem sido associado ao declínio funcional, diminuição da qualidade de vida e aumento do isolamento social na população idosa. Este estudo teve como objetivo analisar preditores do medo de cair em pessoas idosas portuguesas residentes na comunidade. Foi desenvolvido um estudo transversal com uma amostra de conveniência de 98 participantes (57,1% mulheres; média etária 74,07 ± 8,74 anos). O protocolo de coleta de dados incluiu um questionário sociodemográfico e de saúde, a Escala de Confiança no Equilíbrio específica para a Atividade, a Escala de Ansiedade e Depressão Hospitalar, a Escala Breve de Redes Sociais de Lubben, o Timed Up and Go, e o Teste de Sentar e Levantar Cinco Vezes. Os dados foram analisados com recurso à estatística descritiva e inferencial. Os resultados indicam que no gênero feminino (p = 0.01), a percepção de saúde física boa (p = 0.01) e moderada (p = 0.02) e os sintomas de depressão (p ≈ 0.00) são preditores do medo de cair. Controlar estes preditores é um aspecto fundamental para a promoção da independência das pessoas idosas, minimizando as consequências associadas ao medo de cair.


Abstract The fear of falling constitutes a real risk factor for falls. It has also been associated with functional decline, decreased quality of life and increased social isolation among the elderly population. This study analyzed predictors of the fear of falling in elderly Portuguese people that live in the community. A cross-sectional study with a convenience sample of 98 participants (57.1% women; mean age 74.07 ± 8.74 years) was conducted. Data were collected with a protocol which includes a questionnaire for sociodemographic and health information, the Activity Specific Balance Confidence Scale, the Hospital Anxiety and Depression Scale, the Lubben Social Network Scale-6, the Timed Up and Go, and Five Times Sit to Stand Test. Data were analyzed using descriptive and inferential statistics. The results showed that female gender (p = 0.01), the perception of good (p = 0.01) and moderate (p = 0.02) physical health and the symptoms of depression (p ≈ 0.00) are predictors of fear of falling. Controlling these predictors is a fundamental aspect for promoting the independence of elderly people minimizing the consequences that are associated with the fear of falling.

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