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J Sci Food Agric ; 101(13): 5574-5582, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33709417


BACKGROUND: The optimal use of feed resources must be considered by most livestock farmers. The use of low-cost agricultural by-products and the processing of these materials is one possible solution in this respect. One such compound is edible button mushroom waste (EM), a large amount of which is produced annually in the mushroom production cycle worldwide. RESULTS: Bulk density 100 of EM was smaller than the other groups. These changes also applied to alfalfa for bulk density, which was higher than the replaced waste. The dry matter solubility of EM was higher than that of alfalfa hay, whereas the ash solubility rate for EM was greater compared to alfalfa. Replacing up to 210 g kg-1 alfalfa with EM did not affect the production of purine derivatives, microbial protein, nitrogen excreted in urine and feces, and retained nitrogen, although the organic matter digestibility (OMD) increased, whereas the crude protein digestibility and neutral detergent fiber (NDF) decreased (P < 0.05). Fermentation potential, gas production rate, metabolizable energy and short-chain fatty acids were increased. On replacing up to 210 g kg-1 alfalfa with EM, the diet OMD increased, whereas the crude protein and NDF digestibility decreased (P < 0.05). CONCLUSION: EM usage in the experimental diets did not affect the production of purine derivatives, microbial protein, nitrogen excreted in urine and feces, and retained nitrogen. The physical properties, chemical composition and nutritional value of EM, as well as its low cost, show that it can be used as an alternative part of the diet forage in the ruminant's diet. © 2021 Society of Chemical Industry.

Agaricus/química , Nitrogênio/metabolismo , Ovinos/metabolismo , Resíduos/análise , Agaricales/química , Agaricales/metabolismo , Agaricus/metabolismo , Ração Animal/análise , Animais , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Bactérias/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Dieta/veterinária , Digestão , Ácidos Graxos Voláteis/metabolismo , Microbioma Gastrointestinal , Medicago sativa/química , Medicago sativa/metabolismo , Nutrientes/química , Nutrientes/metabolismo , Biossíntese de Proteínas , Ovinos/crescimento & desenvolvimento , Ovinos/microbiologia
Cell Mol Biol (Noisy-le-grand) ; 64(1): 47-51, 2018 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-29412793


Citrus Limon Oil (CLO) is known as antioxidant resource and contains limonoids, flavonoids and phenolic compounds. This study was conducted to investigate the effects of different levels of CLO on blood parameters and antioxidant status in Afshari ewes. Six adults Afshari ewes (3-4 years old and 51±5 kg) were randomly allocated to 3×3 Latin square design with three diets in 21 days period. Dietary treatments included:1) control diet, 2) control diet with 200 mg/day CLO, and 3) control diet with 400 mg/day CLO. To evaluate the antioxidant effect of the CLO, sustainable elimination of free radicals by DPPH and ABTS methods were used. The antioxidant activity of essential oils in DPPH method at doses of 32.5, 45, 130, 260 and 520 mg/ml were 9, 16, 31, 49 and 89%, respectively. Also, antioxidant activity of essential oils in ABTS method at doses of 32.5, 45, 130, 260 and 520 mg/ml were 49, 73, 81, 89 and 95%, respectively. CLO treatments did not affect glucose, blood urea nitrogen, albumin, total protein, low density lipoprotein, while improved the concentration of high-density lipoprotein (P>0.01). Results showed that supplementation with CLO significantly decreased (P<0.01) cholesterol, triglycerides and very low density lipoprotein concentrations compared with control. There was no significant difference in analyzed blood bio-chemicals and serum enzymes level between different antioxidant activity methods and groups, suggesting general well-being of ewes. These results suggest that, CLO supplementation had a positive impact on blood traits and antioxidant status of the Afshari ewes.

Antioxidantes/farmacologia , Citrus , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Ovinos/sangue , Animais , Enzimas/sangue , Feminino , Lipídeos/sangue
J Equine Vet Sci ; 64: 107-111, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-30973145


The source and history of horse domestication have been studied for decades in biology and archeology. The aim of this study was to evaluate the genetic structure and the genetic relationship between six breeds of native horses using mitochondrial D-loop region. Blood samples were collected from 96 horses. Total DNA was extracted, and 430 bp of D-Loop region (hyper variable) was amplified and sequenced using Sanger sequencing methods. The analysis of data led to identify 48 polymorphic sites that create 52 haplotypes. The plotted phylogenic tree for haplotypes of Iranian native horses is placed in the 11 haplogroups including A, B, C, E, G, I, L, M, N, P, and Q. Genetic and haplotype diversity values obtained were 0.0233 and 0.980, respectively. Nucleotide diversity (Pi) was observed between 0.0172 and 0.0242 in populations. In addition, the average number of nucleotide differences (k) ranged from 6.0 (Darehshori or Qashqai horse [DAH]) to 8.5 (Kord horse [KOH]) with an average value of 7.8. The highest and lowest genetic differentiates were observed between KOH and DAH (Fst = 0.193) and between North West native horse and horse riding club in North West (Fst = 0.003), respectively. Tajima D of 0.441 was obtained for all samples which was not significant (P > .01). The results indicate high genetic variety and numerous maternal lines in native horses of Iran.