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1.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3133-3143, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467705

RESUMO

To study the effect of mineral Chloriti Lapis on pulmonary metabolites and metabolic pathways in lung tissues of rats with acute exacerbation of chronic obstructive pulmonary disease(AECOPD). The AECOPD rat model of phlegm heat syndrome was replicated by the method of smoking combined with Klebsiella pneumoniae infection. Except for using UPLC-Q-TOF-MS analysis, SPSS 18.0, SIMCA 13.0 and other software were also used for statistical analysis. Through literature search and online database comparison, the differential metabolites were identified, and the possible metabolic pathways were analyzed. After 15 days of administration, PLS-DA analysis was carried out on lung tissue samples of rats in each group. The results showed that the metabolic profiles of lung tissues of rats in each group could be well separated, which indicated that Chloriti Lapis and aminophylline had significant intervention effect on the lung metabolic profile of rats with AECOPD. Moreover, the metabolic profile of Chloriti Lapis group was closer to that of control group, and the intervention effect was better than that of aminophylline group. As a result, 15 potential differential metabolites were identified: phytosphingosine, sphinganine, tetradecanoylcarnitine, L-palmitoylcarnitine, elaidic carnitine, lysoPC[18∶2(9Z,12Z)], lysoPC(16∶0), lysoPC[18∶1(9Z)], lysoPC(18∶0), stearic acid, lysoPC(15∶0), arachidonic acid, docosapentaenoic acid, linoleic acid and palmitic acid. Among them, Chloriti Lapis could significantly improve the levels of 10 differential metabolites of phytosphingosine, tetradecanoylcarnitine, L-palmitoylcarnitine, elaidic carnitine, lysoPC[18∶2(9Z,12Z)], lysoPC(16∶0), lysoPC[18∶1(9Z)], stearic acid, lysoPC(15∶0), and palmitic acid(P<0.05). The intervention effect of Chloriti Lapis group was better than that of aminophylline group. Analysis of metabolic pathways showed that there were 8 possible metabolic pathways that could be affected, and three of the most important metabolic pathways(pathway impact>0.1) were involved: linoleic acid metabolism, arachidonic acid metabolism, and sphingolipid metabolism. Chloriti Lapis had obvious intervention effects on lung tissue-related metabolites and metabolic pathways in rats with AECOPD, and the effect was better than that of aminophyllinne.


Assuntos
Medicina Tradicional Chinesa , Doença Pulmonar Obstrutiva Crônica , Animais , Pulmão , Metabolômica , Minerais , Ratos
2.
Zhongguo Zhong Yao Za Zhi ; 46(14): 3694-3704, 2021 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-34402294

RESUMO

The effects of Chloriti Lapis on metal elements in plasma and lung tissue of acute exacerbation of chronic obstructive pulmonary disease( AECOPD) rats were studied. The rat AECOPD model with phlegm heat syndrome was established by smoking combined with Klebsiella pneumoniae infection. After the rats were treated by Chloriti Lapis,the contents of metal elements in plasma and lung tissue were determined by inductively coupled plasma-optical emission spectroscopy( ICP-OES) and inductively coupled plasma mass spectrometry( ICP-MS). The changes in the contents of metal elements were analyzed by SPSS 18. 0. Further,the correlations of differential metal elements( including Cu/Zn ratio) with differential metabolites in plasma,lung tissue and urine of AECOPD rats treated with Chloriti Lapis were analyzed. The results showed that Chloriti Lapis significantly up-regulated the contents of Fe,Al,Mn,Cu,Zn,Sn( P<0. 05),V,Co( P< 0. 01) and Cu/Zn ratio( P< 0. 05),and significantly down-regulated the contents of Ti( P< 0. 05)and Pb( P<0. 05) in the model rat plasma. It significantly increased the content of Be( P<0. 05) and decreased the contents of Mg,Ti and Al( P<0. 01) in model rat lung tissue. The element profiles of normal group,model group and Chloriti Lapis group can be well separated. Chloriti Lapis group and other groups were clustered into two categories. The taurine in plasma and phytosphingosine in lung tissue had the strongest correlations with differential metal elements. The Fe,Al,Mg,Be,Ti,V,Mn,Cu,Zn,Sn,and Co in Chloriti Lapis may directly or indirectly participate in the intervention of AECOPD rats. This group of metal elements may be the material basis of Chloriti Lapis acting on AECOPD rats,and reduce the Cu/Zn value in vivo. It was further confirmed that Chloriti Lapis could interfere with the metabolic pathways of taurine and hypotaurine in plasma and urine as well as the sphingolipid metabolism pathway in lung tissue of AECOPD rats. In addition,this study confirmed that long-term smoking can cause high-concentration Cd accumulation in the lung and damage the lung tissue.


Assuntos
Doença Pulmonar Obstrutiva Crônica , Oligoelementos , Animais , Pulmão , Medicina Tradicional Chinesa , Minerais , Ratos , Análise Espectral , Oligoelementos/análise
3.
J Chromatogr Sci ; 56(10): 895-902, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-29982351

RESUMO

Leaves of Platycladus orientalis have been used as blood cooling and homeostatic therapy for thousands of years in traditional Chinese medicine. Emerging evidences of modern pharmacology have proved flavonoids as the key elements responsible for the efficacies. However, there has been no report on pharmacokinetic study of the flavonoids from Platycladus orientalis leaves extract. In this study, a sensitive and rapid ultra-flow liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of amentoflavone, afzelin, hinokiflavone and quercitrin in rat plasma. The four flavonoids and luteolin (internal standard, IS) were recovered from rat plasma by methanol-ethyl acetate (v:v, 50:50). Chromatographic separation was performed on a C18 column with gradient elution. Our results showed that the recoveries from spiked control samples were more than 85% for all analytes and IS. The relative standard deviations of intra-day and inter-day precision were within 15% while the REs ranged from -6.6% to 8.0%. The validated method in this study was successfully applied to pharmacokinetic study in healthy rats after oral administration of P. orientalis leaves extract.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/sangue , Flavonoides/farmacocinética , Traqueófitas/química , Animais , Estabilidade de Medicamentos , Flavonoides/química , Limite de Detecção , Modelos Lineares , Masculino , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacocinética , Folhas de Planta/química , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem/métodos
4.
Artigo em Inglês | MEDLINE | ID: mdl-29738966

RESUMO

Liguzinediol (2,5-dihydroxymethyl-3,6-dimethylpyrazine, LZDO) is a potential agent for the low-risk treatment of heart failure. 2-N-acetylcysteine-LZDO (2-NAC-LZDO) and 2-cysteine-LZDO (2-Cys-LZDO) are major LZDO metabolites found in the pharmacokinetic studies of rats and beagle dogs. To elucidate the biotransformation pathway and related enzymes, an incubation system with 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as a cofactor and N-acetylcysteine (NAC) as a trapping agent was established using liver cytosol. An ultra-flow liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UFLC-QTOF-MS) method was used to identify the major metabolites. 2-NAC-LZDO could be detected among four species (humans, monkeys, dogs, and rats) and is the dominant metabolite in human liver cytosol (HLC). The sulfotransferase (SULT) inhibitors 2,6-dichloro-4-nitrophenol (DCNP) and quercetin at a concentration of 1 µM, suppressed 2-NAC-LZDO formation in HLC by 87 and 46%, respectively. This result suggested that sulfotransferase was involved in 2-NAC-LZDO formation. The metabolism of LZDO in different species indicated that SULT activity in dogs, rats, and monkeys was higher than that in humans. Further SULT phenotyping revealed that SULT1A1 is the predominant enzyme involved in the sulfation of LZDO. The underlying mechanism for the biotransformation of LZDO was demonstrated. The potential pathway is via the sulfation of LZDO to form sulfate, and the spontaneous cleavage of the sulfate group to generate highly reactive electrophilic cations, which can bind to NAC to form the major metabolites.


Assuntos
Pirazinas/metabolismo , Sulfotransferases/metabolismo , Espectrometria de Massas em Tandem/métodos , Acetilcisteína/química , Animais , Biotransformação , Catálise , Técnicas de Cultura de Células/métodos , Cromatografia Líquida de Alta Pressão/métodos , Cães , Haplorrinos , Humanos , Fígado/citologia , Fígado/metabolismo , Metaboloma , Metabolômica/métodos , Estrutura Molecular , Pirazinas/química , Ratos , Transdução de Sinais
5.
Anal Chem ; 90(9): 5678-5686, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29644847

RESUMO

The currently utilized ligand fishing for bioactive molecular screening from complex matrixes cannot perform imaging screening. Here, we developed a new solid-phase ligand fishing coupled with an in situ imaging protocol for the specific enrichment and identification of heat shock protein 90 (Hsp 90) inhibitors from Tripterygium wilfordii, utilizing a multiple-layer and microkernel-based mesoporous nanostructure composed of a protective silica coating CdTe quantum dot (QD) core and a mesoporous silica shell, i.e., microkernel-based mesoporous (SiO2-CdTe-SiO2)@SiO2 fluorescent nanoparticles (MMFNPs) as extracting carries and fluorescent probes. The prepared MMFNPs showed a highly uniform spherical morphology, retention of fluorescence emission, and great chemical stability. The fished ligands by Hsp 90α-MMFNPs were evaluated via the preliminary bioactivity based on real-time cellular morphology imaging by confocal laser scanning microscopy (CLSM) and then identified by mass spectrometry (MS). Celastrol was successfully isolated as an Hsp 90 inhibitor, and two other specific components screened by Hsp 90α-MMFNPs, i.e., demecolcine and wilforine, were preliminarily identified as potential Hsp 90 inhibitors through the verification of strong affinity to Hsp 90 and antitumor bioactivity. The approach based on the MMFNPs provides a strong platform for imaging screening and discovery of plant-derived biologically active molecules with high efficiency and selectivity.


Assuntos
Compostos de Cádmio/química , Corantes Fluorescentes/química , Nanopartículas/química , Imagem Óptica , Dióxido de Silício/química , Telúrio/química , Tripterygium/química , Compostos de Cádmio/síntese química , Compostos de Cádmio/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/farmacologia , Proteínas de Choque Térmico HSP90/antagonistas & inibidores , Proteínas de Choque Térmico HSP90/metabolismo , Humanos , Células MCF-7 , Tamanho da Partícula , Porosidade , Dióxido de Silício/síntese química , Dióxido de Silício/farmacologia , Relação Estrutura-Atividade , Propriedades de Superfície , Telúrio/farmacologia
6.
J Pharm Biomed Anal ; 141: 19-31, 2017 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-28414971

RESUMO

In the present study, the chemical compositions of Atractylodes macrocephala Koidz. (AMK) were analyzed systematically and influence of sulfur fumigation on the chemical profiles was evaluated by ultrafast flow liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UFLC-QTOF-MS) combined with multivariate statistical analysis. 52 components were detected from non-fumigated AMK (NF-AMK) and 28 components were newly produced after sulfur fumigation, out of which 59 major peaks were identified. The concentrations of 20 compounds significantly decreased and 37 compounds obviously increased. The potential structural transformation mechanism of terpenoids was explored to illustrate the correlation of the components contents before and after sulfur fumigation. Eight sulfur-containing/dehydrated-integrated atractylenolides that evolved from the NF-AMK were screened out as potential characteristic chemical markers to examine the post-harvest handling procedures of commercial AMK with excessive sulfur fumigation and maintain consistent quality.


Assuntos
Espectrometria de Massas , Atractylodes , Cromatografia Líquida de Alta Pressão , Fumigação , Análise Multivariada , Enxofre
7.
Chem Commun (Camb) ; 52(79): 11779-11782, 2016 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-27722615

RESUMO

A combined specific enrichment and highly efficient solid-phase tagging approach is presented for heparin detection using boronic acid-functionalized mesoporous silica nanospheres as extraction sorbents and nanoscale reactors. It exhibits a faster reaction time (only 6 min), higher tagging-product purity and lower applicable sample concentration compared with liquid-phase tagging.

8.
Anal Chim Acta ; 932: 60-8, 2016 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-27286770

RESUMO

The current widely utilized polymer or C8, C18 end-capped material-based sorbents for solid-phase extraction could not capture alkaloids well only based on "like dissolves like" principle. In this paper, a layer-by-layer functionalized porous Zinc sulfide nanospheres-based solid-phase extraction (SPE) combined with liquid chromatography time-of-flight/mass spectrometry (LC-TOF/MS) and gas chromatography-mass spectrometry (GC-MS) was developed for the specific enrichment and identification of alkaloids from complex matrixes, Crinum asiaticum var. sinicum crude extracts. The functionalized porous Zinc sulfide nanospheres were prepared by the amidation reaction of poly-(acrylic acid) (PAA) homopolymer with amino groups onto the porous ZnS nanospheres. Tandem LC-TOF/MS spectrometry presented that the almost all of the twenty-three main peaks in elution fraction from the SPE could be inferred as alkaloids with ion of mass according to the nitrogen rule and hit formula with Peak View1.2@software from AB SCIEX, and seven alkaloids including two new found chemical entities were directly identified from their GC-MS spectra and retention indices. We believe that this SPE protocol can also be utilized in the future to selectively enrich alkaloids from extracts of other plant species.


Assuntos
Alcaloides/química , Alcaloides/isolamento & purificação , Crinum/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Nanosferas/química , Extração em Fase Sólida/métodos , Sulfetos/química , Compostos de Zinco/química , Porosidade
9.
J Ethnopharmacol ; 184: 187-95, 2016 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-26965366

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Annona squamosa Linn (Annonaceae) is a commonly used and effective traditional Chinese medicine (TCM) especially in the South China. The seeds of Annona squamosa Linn (SAS) have been used as a folk remedy to treat "malignant sores" (cancer) in South of China, but they also have high toxicity on human body. AIM OF THE STUDY: To discover the potential biomarkers in the mice caused by SAS. MATERIALS AND METHODS: We made metabonomics studies on the toxicity of SAS by ultraperformance liquid-chromatography high-definition mass spectrometry coupled with pattern recognition approach and metabolic pathways analysis. RESULTS: The significant difference in metabolic profiles and changes of metabolite biomarkers between the Control group and SAS group were well observed. 11 positive ions and 9 negative ions (P<0.05) were indicated based on UFLC-QTOF-HDMS. The metabolic pathways of SAS group are discussed according to the identified endogenous metabolites, and eight metabolic pathways are identified using Kyoto Encyclopedia of Genes and Genomes (KEGG). CONCLUSIONS: The present study demonstrates that metabonomics analysis could greatly facilitate and provide useful information for the further comprehensive understanding of the pharmacological activity and potential toxicity of SAS in the progress of them being designed to a new anti-tumor medicine.


Assuntos
Annona , Metaboloma/efeitos dos fármacos , Extratos Vegetais/toxicidade , Animais , Biomarcadores/urina , Cromatografia Líquida/métodos , Feminino , Fígado/efeitos dos fármacos , Fígado/patologia , Metabolômica , Camundongos Endogâmicos ICR , Reconhecimento Automatizado de Padrão , Sementes , Espectrometria de Massas por Ionização por Electrospray
10.
Artigo em Inglês | MEDLINE | ID: mdl-26680326

RESUMO

3,4-Divanillyltetrahydrofuran is the main active ingredient of nettle root which can increase steroid hormones in the bloodstream for many of bodybuilders. To better understand its pharmacological activities, we need to determine its pharmacokinetic profiles. In this study, a rapid and sensitive ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method has been developed for the determination of 3,4-divanillyltetrahydrofuran in the plasma of rats. Chromatographic separation was performed on a C18 column at 40°C, with a gradient elution consisting of methanol and water containing 0.3% (v/v) formic acid at a flow rate of 0.8mL/min. The detection was performed using an electrospray triple-quadrupole MS/MS via positive ion multiple reaction monitoring mode. The lower limits-of-quantification determined were 0.5ng/mL. The intra- and inter-day precision (RSD%) was found to be within 15% and the accuracy (RE%) ranged from -4.0% to 7.0%. This simple yet sensitive method was fully validated and could be successfully applied to the study on pharmacokinetics of 3, 4-divanillyltetrahydrofuran.


Assuntos
Cromatografia Líquida/métodos , Furanos/farmacocinética , Lignina/farmacocinética , Espectrometria de Massas em Tandem/métodos , Animais , Área Sob a Curva , Furanos/sangue , Meia-Vida , Lignina/sangue , Masculino , Ratos , Padrões de Referência , Reprodutibilidade dos Testes
11.
Toxins (Basel) ; 8(1)2015 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-26712791

RESUMO

Recent studies have examined lipophilic marine toxins (LMTs) in shellfish and toxic algae worldwide, but the occurrence and seasonal variations of LMTs in commercial clams (including Mactra veneriformis, Ruditapes philippinarum, Meretrix meretrix, and Cyclina sinensis) at their major culturing area in Jiangsu, China, remain largely unexplored. In this study, a new solid phase extraction (SPE) in combination with an ultra-fast liquid chromatography and triple-quadrupole linear ion trap mass spectrometry (UFLC-TQ-MS) method was developed to determine the presence of 10 typical LMTs (okadaic acid (OA), yessotoxins (YTXs), azaspiracids (AZA1-3), pectenotoxins (PTX2), gymnodimine (GYM), dinophysistoxins (DTX1&2), and spirolides (SPX1)) in the aforementioned four clam matrices. After confirmation of its sensitivity and precision, this method was used to evaluate the amounts of LMTs in clam samples harvested in five aquaculture zones of the Jiangsu coastal area. Monthly variations of GYM, PTX2, OA, and DTX1&2 in 400 clam samples from the sample areas were determined from January 2014 through August 2015. Peak values were observed during May and August. This is the first systematic report of LMTs detected in clam samples harvested in Jiangsu. Follow-up research and the implementation of protective measures are needed to ensure the safety of clams harvested in this area.


Assuntos
Bivalves/química , Toxinas Marinhas/análise , Animais , China , Cromatografia Líquida , Monitoramento Ambiental , Contaminação de Alimentos/análise , Estações do Ano , Espectrometria de Massas em Tandem
12.
J Chromatogr Sci ; 53(8): 1280-8, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25680683

RESUMO

An HPLC method was employed to create an impurity profile for liguzinediol as an active pharmaceutical ingredient (API), which resulted in the identification of two related impurities. Therefore, in order to improve the quality control of the liguzinediol-API, we identified and then developed a method for quantifying the two impurities (impurity-1 and impurity-2) by LC-TOF-MS-MS and then chemically synthesized them for further studies. Based on spectral data from IR, MS, (1)H and (13)C NMR, the structures of impurity-1 and impurity-2 were characterized as 2-hydroxymethyl-3,6-dimethylpyrazine and 2-hydroxymethyl-3,5,6-trimethylpyrazine, respectively. We further validated the method according to the International Conference on Harmonization guidelines to demonstrate the sensitivity, precision, linearity, accuracy and stability of the method described. In addition, the potential mechanisms underlying formation of impurity-1 and impurity-2 in the liguzinediol-API are discussed in detail.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Pirazinas/análise , Pirazinas/química , Contaminação de Medicamentos , Estabilidade de Medicamentos , Modelos Lineares , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Razão Sinal-Ruído
13.
Artigo em Inglês | MEDLINE | ID: mdl-25078861

RESUMO

Ultra-flow liquid chromatography/quadrupole-time-of-flight mass spectrometry (UFLC/Q-TOF MS) method combined with metabolitepilot(MT) software was used for analysis of the metabolites of liguzinediol in dogs. Urine, bile, feces and plasma samples were collected after intravenous administration of 8 mg/kg liguzinediol to healthy dogs. Besides liguzinediol, seven metabolites were detected and identified by UFLC/Q-TOF MS method. The results showed that liguzinediol had some main metabolic pathways in dogs including oxidation, sulfation, cysteine conjugation, N-acetylcysteine conjugation and glucuronidation.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Pirazinas/química , Pirazinas/metabolismo , Espectrometria de Massas em Tandem/métodos , Animais , Bile/química , Cães , Fezes/química , Redes e Vias Metabólicas , Pirazinas/análise
14.
J Pharm Biomed Anal ; 94: 1-11, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24531004

RESUMO

Peperomia dindygulensis, with secolignans (SLs) as major bioactive constituents, is a commonly used traditional folk medicine in mainland China for treatment of stomach, liver, mammary, and esophageal cancers. However, to date, there is no method available for the qualitative and quantitative analyses of SLs in this medicinal plant. The purpose of this study was to establish a sensitive, selective, and reproducible method for rapidly profiling, identifying, and determining SLs in the whole plant of P. dindygulensis. Ultra high-performance liquid chromatography (UHPLC) coupled with ultraviolet detector (UV) and quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS) were used for this analyses. The fragmentation behaviors of different types of SLs were described. A total of thirteen SLs, including two new derivatives, were identified or tentatively characterized in P. dindygulensis samples. In addition, seven major SLs in herbal samples from different regions in China were successfully determined. The method developed in this study is suitable for the qualitative and quantitative analyses of SLs in P. dindygulensis, and may be applicable for determining or identifying SLs from other Pepermia genus plants.


Assuntos
Peperomia/química , Plantas Medicinais/química , China , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Espectrofotometria Ultravioleta/métodos , Espectrometria de Massas em Tandem/métodos
15.
Artigo em Inglês | MEDLINE | ID: mdl-24295907

RESUMO

A rapid and sensitive ultra fast performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of five bioactive secolignans in Peperomia dindygulensis extract, including peperomin A, peperomin B, peperomin C, 4″-hydroxypeperomin B and 4″-hydroxypeperomin C in rat plasma. Arctigenin was used as the internal standard. The separation was performed on an Innovation™ Polar-RP C18 column by a gradient elution within a runtime of 7min. The mobile phase consisted of A (methanol) and B (0.1% formic acid in water) at a flow rate of 0.4mL/min. The detection was accomplished by using positive ion TurboIonSpray ionization in multiple reaction monitoring mode. The method was linear for all analytes over investigated range with all correlation coefficients greater than 0.9972. The lower limits of quantification were 1.1ng/mL for peperomin A, 1.24ng/mL for peperomin B, 1.02ng/mL for peperomin C, 1.91ng/mL for 4″-hydroxypeperomin B and 1.27ng/mL for 4″-hydroxypeperomin C. The intra- and inter-day precision (RSD%) was within 15% and the accuracy (RE%) ranged from -11.7% to 10.3%. This simple and sensitive method was fully validated and successfully applied to the pharmacokinetic study of peperomin A, peperomin B, peperomin C, 4″-hydroxypeperomin B and 4″-hydroxypeperomin C in rat plasma after oral administration of P. dindygulensis extract.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Lignanas/sangue , Peperomia/química , Extratos Vegetais/administração & dosagem , Espectrometria de Massas em Tandem/métodos , Administração Oral , Animais , Estabilidade de Medicamentos , Lignanas/química , Lignanas/farmacocinética , Masculino , Extratos Vegetais/farmacocinética , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
16.
Zhongguo Zhong Yao Za Zhi ; 38(12): 1929-33, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24066586

RESUMO

To establish a fingerprint spectrum for Atractylodis Macrocephalae Rhizoma stir-fried with wheat bran based on UFLC/Q-TOF-MS, and make a principal component analysis (PCA) with Markview software, in order to compare the changes of components between raw and processed Atractylodis Macrocephalae Rhizoma with raw wheat bran as the blank. The results showed that the changed in components raw Atractylodis Macrocephalae Rhizoma and Atractylodis Macrocephalae Rhizoma stir-fried with wheat bran were apparently observed by PCA. Six compounds were identified to have significant changes in mass fraction before and after being stir-fried, namely atractylenolide-I, atractylenolide-II, atractylenolide-III, atractylentrid, atractylon and an unknown compound. Among them, atractylenolide-I and atractylenolide-II generated from dehydration and dehydrogenation of atractylenolide-III may be the material base of Atractylodis Macrocephalae Rhizoma stir-fried with wheat bran for strengthening spleen.


Assuntos
Atractylodes/química , Cromatografia Líquida/métodos , Fibras na Dieta , Lactonas/análise , Espectrometria de Massas , Análise de Componente Principal , Sesquiterpenos/análise
17.
J Pharm Biomed Anal ; 62: 187-90, 2012 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-22277354

RESUMO

Ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/QTOF MS) was employed to investigate the in vivo metabolism of liguzinediol. Urine, bile, feces and plasma samples were collected after intravenous administration of 10mg/kg liguzinediol to healthy rats. Altogether seven metabolites were detected and tentatively identified based on the characteristics of their protonated ions. The metabolites were mainly transformed by four main metabolic pathways including oxidation, sulfation, glycine conjugation and glucuronidation.


Assuntos
Cromatografia Líquida/métodos , Pirazinas/metabolismo , Espectrometria de Massas em Tandem/métodos , Animais , Masculino , Ratos , Ratos Sprague-Dawley
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