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1.
Oncogene ; 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31831835

RESUMO

The original version of this Article omitted the following from the Acknowledgements: Professor Stebbing sits on SABs for Celltrion, Singapore Biotech, Vor Biopharma, TLC Biopharmaceuticals and Benevolent AI, has consulted with Lansdowne partners, Vitruvian and Social Impact Capital and Chairs the Board of Directors for BB Biotech Healthcare Trust and Xerion Healthcare. This has now been corrected in both the PDF and HTML versions of the Article.

2.
Oncogene ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31754213

RESUMO

EGFR-mutant non-small-cell lung cancer (NSCLC) patients inevitably develop drug resistance when treated with EGFR tyrosine kinase inhibitors (TKIs). Systematic genetic analysis is important to understand drug-resistant mechanisms; however, the clinical significance of co-occurring genetic alterations at baseline, co-acquired mutations at progressive disease (PD), and the clonal evolution remain underinvestigated. We performed targeted sequencing of pre-treatment and PD tumor samples from 54 EGFR-mutant NSCLC patients. Ten additional patients were sequenced using whole-exome sequencing to infer the clonal evolution patterns. We observed a domain-dependent effect of PIK3CA mutation at baseline on patient progression-free survival (PFS). In addition, at baseline, 9q34.3/19p13.3 (NOTCH1/STK11/GNA11) showed a co-deletion pattern, which was associated with a significantly worse PFS (p = 0.00079). T790M-postive patients with other concurrent acquired oncogenic mutations had a significantly shorter PFS (p = 0.005). Besides acquired T790M mutation, chromosomal instability (CIN) related genes, including AURKA and TP53 alterations, were the most frequently acquired events. CIN significantly increased during TKI treatment in T790M-negative patients and is a candidate resistance mechanism to the first-generation TKIs. Clonal evolution analyses suggest that the composition and relationship among resistant subclones, particularly relationship with T790M subclone, affect patients' outcomes. Overall, our findings of novel co-occurring alterations and clonal evolution patterns can be served as predictive biomarkers to stratify patients and help to better understand the drug-resistant mechanism to TKIs.

3.
Onco Targets Ther ; 12: 6549-6554, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31616163

RESUMO

Objective: Anlotinib is an oral novel multi-target tyrosine kinase inhibitor targeting vascular endothelial growth factor receptor, fibroblast growth factor receptor, platelet-derived growth factor receptor, and stem cell factor receptor (c-Kit). The aim of this study was to evaluate the efficacy and safety of anlotinib treatment in advanced lung cancer in the real world. Methods: We evaluated the efficacy and toxicity of apatinib in patients with previously treated advanced lung cancer from 2018 to 2019 in Zhejiang Cancer Hospital. Survival analysis was performed by the Kaplan-Meier method. Results: Fifty-eight patients were included in the present study. Thirty-one of these patients received anlotinib treatment as a third line and 27 patients received further therapy.  All 58 patients had therapeutic evaluation and 46 patients acquired progression-free survival evaluation. Ten patients achieved partial response (PR), and 36 achieved stable disease (SD), representing a response rate of 17.2% and a disease control rate of 77.6%. Median progression-free survival was 3.3 months (95% CI 1.595-5.071). The toxicities associated with anlotinib were generally acceptable with a total grade 3/4 toxicity of 5.2%. The toxicities of anlotinib were generally tolerated and the common toxicities were hand-foot syndrome and hypertension. Conclusion: In the third-line or more-line treatment of advanced lung cancer, anlotinib appears to have some activity when utilized as a salvage treatment. Adverse reactions are controllable.

4.
Obesity (Silver Spring) ; 27(11): 1898-1905, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31549787

RESUMO

OBJECTIVE: This study aimed to examine the latest prevalence of abdominal obesity in China based on nationally representative data. METHODS: A stratified, multistage, random sampling method was used to obtain a nationally representative sample of residents aged ≥ 18 years from 31 provinces in mainland China from October 2012 to December 2015. Abdominal obesity was defined as a waist circumference ≥ 90 cm for men and a waist circumference ≥ 85 cm for women. RESULTS: The data of 441,306 participants were analyzed. The prevalence of abdominal obesity was 29.1% (28.6% in men and 29.6% in women); the number of adults with abdominal obesity was estimated to be 277.8 million (approximately 140.1 million in men and 137.7 million in women). The prevalence of abdominal obesity varied considerably among provinces. In general, the prevalence of abdominal obesity was higher in the northern, lower in the southern, higher in the western, and lower in the eastern areas of China. Besides the regional disparities, the prevalence of abdominal obesity varied greatly among different population subgroups. CONCLUSIONS: Abdominal obesity is highly prevalent among Chinese adults. National and provincial obesity control and prevention strategies should be public health priorities in China. To better control obesity, the underlying reasons for the regional disparities need to be addressed.

5.
PLoS One ; 14(8): e0220670, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31369639

RESUMO

Somatic mutations of MET gene are emerging as important driver mutations for lung cancers. To identify the common clinicopathological features of MET exon 14 skipping mutations and amplification and clarify whether the two MET gene alterations cause protein overexpression were investigated using 196 lung cancer samples of Taiwan through real time-qPCR/sequencing, fluorescence in situ hybridization, and immunohistochemistry. The two MET gene alterations are both present in low frequency, ~1%, in the studied lung cancer population of Taiwan. MET exon 14 skipping mutations were identified from two early-stage patients, who were both relatively advanced in age, and did not carry other driver mutations. One was an adenocarcinoma and the other was a rare carcinosarcoma. Three gene amplifications cases were identified. Neither of the two MET gene alterations would lead to protein overexpression; hence, direct detection in nucleic acid level would be a preferred and straightforward solution for the identification of skipping mutations. The presence of MET exon 14 mutations in minor histological types of lung cancers urge to extend screening scope of this mutation in lung cancer and treatment response evaluation in clinical trials. These would be important next steps for the success of MET target therapy in clinical practice.

6.
BMJ Open ; 9(7): e026844, 2019 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-31326927

RESUMO

INTRODUCTION: Multiple rehabilitation therapies have been reported to be effective for poststroke balance impairment. However, the comparative effectiveness of these rehabilitation therapies is still unclear. Therefore, the aim of this study is to summarise evidence and identify the most effective rehabilitation therapy for poststroke balance impairment. METHODS AND ANALYSIS: The following databases will be searched: China Biology Medicine, China National Knowledge Infrastructure, Wan Fang Data, the Chinese Science and Technology Periodical Database, Medline, Excerpt Medical Database (EMBASE), Web of Science, the Cochrane Library, from inception to June 2019. All randomised controlled trials that have used rehabilitation interventions to treat poststroke balance impairment will be included. The primary outcomes are the Berg Balance Scale, the Fugl-Meyer Assessment (balance), the Postural Assessment Scale for Stroke, as well as the function in sitting test, the Sitting Balance Scale, the Ottawa Sitting Scale, the Activities-specific Balance Confidence Scale, the Overall Balance Index and the Brunel Balance Assessment. The secondary outcomes include the Barthel Index, the Functional Ambulation Category Scale, fall rates, the Timed Up and Go test, the MOS 36-Item Short-Form Health Survey, and adverse events. To ensure that all relevant studies are included without personal bias, study selection, data extraction and quality assessment will be performed independently by two reviewers. Risk of bias will be assessed with the Cochrane risk of bias assessment tool. Review Manager V.5.3 software will be used to make bias risk diagram and pairwise meta-analysis, while network data synthesis will be performed using WinBUGS V.1.4.3 and R software. ETHICS AND DISSEMINATION: Ethics approval is not required for systematic review and network meta-analysis. The results will be submitted to a peer review journal or at a conference. TRIAL REGISTRATION NUMBER: PROSPERO (CRD 42018107441).

7.
Dalton Trans ; 48(24): 8911-8919, 2019 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-31143896

RESUMO

A water-stable three-dimensional (3D) metal-organic framework (MOF) of {[Tb(Cmdcp)(H2O)3]2(NO3)2·5H2O}n (1, H3CmdcpBr = N-carboxymethyl-(3,5-dicarboxyl)pyridinium bromide) has been synthesized and characterized. MOF 1 is highly emissive, giving rise to green luminescence that can be quenched by Fe3+ due to the partial overlap of its excitation spectrum with the absorption spectrum of Fe3+. The subsequent introduction of ascorbic acid (AA) leads to the reduction of Fe3+ into Fe2+, accompanied by the near-entire recovery of MOF 1 emission. The density functional theory (DFT) calculation results support the proposed mechanism. Such a sensing cycle is further transferable to urine and serum samples with a satisfactory near-quantitative recovery, highlighting its good potential in biologically relevant applications.

8.
Chem Biol Interact ; 307: 73-81, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31004593

RESUMO

Parkinson's disease (PD), the second most prevalent age-related neurodegenerative disease, occurs as a result of the loss of dopaminergic neurons in the substantia nigra. Long non-coding RNA-p21 (lnc-p21) has been demonstrated to be upregulated in PD. However, its role in PD is unknown. Here, the results showed that lnc-p21 was highly expressed in human neuroblastoma SH-SY5Y cells treated with MPP+. Knockdown of lnc-p21 attenuated the cytotoxicity and cell apoptosis induced by MPP+ as shown by enhanced cell viability, decreased LDH release and cell apoptosis rate, accompanying with reduction of caspase-3 activity and Bax expression, and enhancement of Bcl-2 expression. Furthermore, knockdown of lnc-p21 mitigated MPP+-induced oxidative stress and neuroinflammation, as evidenced by the decrease in ROS generation, increase in SOD activity and decline in TNF-α, IL-1ß and IL-6 levels. Conversely, overexpression of lnc-p21 resulted in the opposite effect. miR-625 was identified as a target of lnc-p21. lnc-p21 overturned the inhibitory effect of miR-625 on MPP+-induced neuronal injury in SH-SY5Y cells. Additionally, lnc-p21 positively regulated TRPM2 expression by targeting miR-625, and knockdown of TRPM2 inhibited MPP+-induced neuronal injury. Overall, our study identified a new lnc-p21-miR-625-TRPM2 regulatory network that lnc-p21 regulated MPP + -induced neuronal injury by sponging miR-625 and upregulating TRPM2 in SH-SY5Y cells, which provide a better understanding for the pathogenesis of PD.


Assuntos
1-Metil-4-fenilpiridínio/toxicidade , Apoptose/efeitos dos fármacos , MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Canais de Cátion TRPM/metabolismo , Regiões 3' não Traduzidas , Antagomirs/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Humanos , Interleucina-1beta/metabolismo , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Neurônios/citologia , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Interferência de RNA , RNA Longo não Codificante/antagonistas & inibidores , RNA Longo não Codificante/genética , RNA Interferente Pequeno/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo , Canais de Cátion TRPM/antagonistas & inibidores , Canais de Cátion TRPM/genética
9.
Biosens Bioelectron ; 135: 173-180, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-31022594

RESUMO

Fluorescent microsphere (FM) is widely used as probe in immunochromatographic assay (ICA). However, the performance of conventional FM is limited because of the aggregation-caused quenching effect. Herein, we compared a kind of conventional FM (DMFFM, loading DMF) with novel aggregation-induced emission FM (AIEFM, loading TCBPE). The fluorescence intensity of DMFFM initially increased and then decreased as the concentrations of the loading DMF increased. The fluorescence intensity of AIEFM increased as the concentrations of the loading TCBPE increased and retained a high value. AIEFM was compared with two commercial FMs purchased from Ocean (OFM) and Merk (MFM). The maximum fluorescence intensity and relative quantum yield of AIEFM was approximately 5 and 4.5 times higher than those of two commercial FMs. We used the novel AIEFM as a probe to improve the sensitivity of ICA. When Escherichia coli O157:H7 was detected as the target, the limit of detection of ICA based on AIEFM, OFM and MFM were 3.98 × 103 CFU/mL, 4.48 × 104 and 2.78 × 104 CFU/mL, respectively. The ICA of AIEFM had 11 and 7 times improvement in sensitivity compared with that of OFM and MFM. Our results could be used as a basis for novel probes in practical ICA applications.


Assuntos
Escherichia coli O157/isolamento & purificação , Corantes Fluorescentes/química , Imunoensaio/instrumentação , Técnicas Biossensoriais/instrumentação , Dimerização , Infecções por Escherichia coli/microbiologia , Fluorescência , Humanos , Microesferas
10.
J Autoimmun ; 100: 27-39, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30954385

RESUMO

In rheumatoid arthritis (RA), imbalanced T cells subsets play a critical role in sustaining chronic inflammatory responses in the synovium. Naïve T cells in RA patients undergo maldifferentiation, including an increase in the effector Th1/Th17 lineage and a reduction in regulatory T (Treg) cells. Upon stimulation, naïve CD4+CD45RO- T cells from RA patients exhibited insufficient expression of Foxp3, which induced a deficiency in Tregs production and an imbalance of Treg/Th17 differentiation. Further mechanistic study indicated that RA T cells failed to produce sufficient levels of the histone acetyltransferase Tip60, leading to reduced acetylation of Foxp3; this, in turn, decreased Foxp3 expression, impaired Treg commitment, and promoted Th17 production. Moreover, in human synovium chimeric mice, suppression of Tip60 activity in healthy T cells promoted tissue infiltration and arthritogenesis, while reconstitution of Tip60 in RA T cells suppressed synovitis and effector T cell infiltration. Our findings link T cell maldifferentiation and tissue infiltration with Tip60-mediated Foxp3 acetylation and identify Tip60 as a potential therapeutic target for suppression of tissue inflammation and autoimmunogenesis in RA.

11.
J Am Heart Assoc ; 8(6): e010454, 2019 03 19.
Artigo em Inglês | MEDLINE | ID: mdl-30871396

RESUMO

Background Data regarding the metabolic risk factors clustering on the risk of left ventricular diastolic dysfunction ( LVDD ) are lacking among people living at high altitude and under hypoxic conditions. In this study, we explored the association between metabolic risk factor clustering and LVDD among the Tibetan population of China. Methods and Results We conducted a cross-sectional survey in a representative sample of 1963 Tibetans in 2014 to 2016. Grading LVDD was based on recommendations for the evaluation of LV diastolic function by echocardiography (2009). The prevalence of LVDD among 1963 participants (mean age: 51.51 years, 41.11% male) was 34.39%. Odds ratios (95% CI ) of LVDD for the 1, 2, and 3 to 5 risk factors clustering were 1.45 (0.96-2.17), 2.68 (1.8-3.98), and 2.9 (1.9-4.43), respectively ( P for trend <0.001). The association between metabolic risk factors clustering and LVDD was much more pronounced in the middle-aged group than in the elderly ( P for interaction=0.0170). High altitude was one of the major independent risk factors for LVDD ; however, habitation altitude had no significant effect on the association between metabolic risk factors and LVDD ( P for interaction=0.1022). The multivariable dominance analysis indicated that abdominal obesity, hypertension, and elevated blood glucose were the significant contributors to LVDD . Conclusions There was a significant positive association between the metabolic risk factor clustering number and LVDD among a population living at high altitude, especially in middle-aged adults. However, habitation altitude itself has no significant effect on the association between metabolic risk factors and LVDD .

12.
Arthritis Rheumatol ; 71(8): 1252-1264, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30802366

RESUMO

OBJECTIVE: Activation of osteoclastogenesis at the bone site in rheumatoid arthritis (RA) is well established. The mechanisms by which circulating osteoclast precursors contribute are still unclear. Peroxisome proliferator-activated receptor γ coactivator 1ß (PGC-1ß) is implicated in transcriptional regulation of osteoclastogenesis in mouse models. This study was undertaken to investigate the contribution of PGC-1ß to circulating osteoclast precursors and its link to bone destruction in RA. METHODS: PGC-1ß expression in RA peripheral blood CD14+ monocytes was increased and showed correlation with joint destruction shown on radiographs. Cells from RA patients or healthy controls were transfected with a lentivirus vector for PGC-1ß gene silencing or overexpression and cultured with macrophage colony-stimulating factor and RANKL. Bone resorption activity, bone-degrading enzymes, and signaling molecules were measured in these mature osteoclasts. RESULTS: Increased nuclear accumulation of PGC-1ß was observed in RA peripheral blood CD14+ monocytes, and these cells had stronger osteoclastogenesis than in healthy controls. PGC-1ß protein expression was positively correlated with radiographic joint destruction (r = 0.396-0.413; all P < 0.05). PGC-1ß knockdown suppressed (51-82% reduction) the expression of cathepsin K, tartrate-resistant acid phosphatase (TRAP), and matrix metalloproteinase 9 (MMP-9), as well as osteoclast differentiation and bone resorption activity. Conversely, PGC-1ß overexpression increased these markers (by 1.5-1.8-fold) and osteoclastogenesis. VIVIT, an inhibitor of NFATc1 activation, inhibited the effect of overexpressed PGC-1ß by reducing cathepsin K, TRAP, and MMP-9 expression. Chromatin immunoprecipitation assay and dual-luciferase reporter gene assay showed PGC-1ß bound to NFATc1 promoter, leading to transcriptional activation. CONCLUSION: Activation of the PGC-1ß/NFATc1 pathway in circulating osteoclast precursors was associated with bone destruction in RA. This may represent a new treatment target.


Assuntos
Artrite Reumatoide/sangue , Reabsorção Óssea/sangue , Fatores de Transcrição NFATC/metabolismo , Osteoclastos/metabolismo , Proteínas de Ligação a RNA/metabolismo , Adulto , Feminino , Humanos , Leucócitos Mononucleares , Fator Estimulador de Colônias de Macrófagos , Masculino , Pessoa de Meia-Idade , Osteogênese/fisiologia , Ligante RANK , Transdução de Sinais/fisiologia
13.
J Epidemiol Community Health ; 73(2): 188-192, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30530521

RESUMO

BACKGROUND: This study aimed to assess the association of high sensitivity C-reactive protein (hs-CRP) with cardiovascular disease (CVD) in middle-aged Chinese population. METHODS: The baseline was collected 2009-2010, and follow-up was conducted in 2016-2017. Data of hs-CRP were from baseline examination and re-examination in 2016-2017 using transmission turbidimetry with a measurement range of 0-42 000. The primary outcome was CVD including coronary heart disease events and stroke events. RESULTS: Among 8688 participants free from CVD (at baseline, mean age, 50.1 years, 3897 were males), there were 189 CVD events, occurred during a median follow-up of 6.34 years (54 685 person-years at risk). From the Kaplan-Meier curve, we found that there was a progressive increase in CVD event rates by hs-CRP tertiles (log-rank test, p<0.001). Baseline hs-CRP was linearly associated with CVD (p for trend=0.015) even after adjusting for known CVD risk factors. Furthermore, the net reclassification improvement when hs-CRP was added to a model based on traditional factors was 7.85% for CVD (p=0.003). In addition, the correlation between change of hs-CRP and CVD was conducted in a subgroup (n=4778). However, we did not find the correlation between hs-CRP change and CVD (correlation coefficient: -0.003, p=0.846). CONCLUSIONS: In the middle-aged Chinese population, hs-CRP was associated with increased risk of developing CVD. Although there was no correlation between hs-CRP change and CVD, the level of hs-CRP was higher at follow-up than baseline even among those with CVD. More attention should be given to those with higher level of hs-CRP for CVD prevention.

14.
Int J Cardiol ; 275: 165-170, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30389296

RESUMO

BACKGROUND: Peripheral arterial disease (PAD) is the third leading cause of atherosclerotic vascular morbidity after coronary heart disease and stroke. Epidemiology data of PAD is very limited in low-income and middle-income countries. A national wide survey was conducted from October 2012 to December 2015 to assess the prevalence of PAD in China. METHODS: Data from the China Hypertension Survey (CHS). In total, 56,000 people aged 35 years or older were enrolled in this sub-survey for PAD, and 30,025 participants were eligible for analysis. Ankle-brachial index was measured using an automated ABI device (WatchBP Office device Microlife, China). PAD was defined by ABI and Edinburgh Claudication Questionnaire. RESULTS AND CONCLUSIONS: Overall, 6.6% (estimated 45.3 million) of the Chinese adult population age 35 years or older had PAD. The weighted prevalence of PAD increased significantly after aged ≥75 years. There were no significant differences in PAD prevalence between urban and rural residents, as well as between males and females. Among individuals with PAD, only 4.9% (95% CI: 0%-10.1%) were aware of their condition, 1.9% (95% CI: 0%-4.0%) received revascularization, and 0.2% (95% CI: 0%-0.4%) achieved ABI >0.9. Older age, Han population, current smokers, education level, hypertension, CAD, diabetes, dyslipidemia, and rural residences all were significantly associated with an increased risk of PAD. In China, there is an increasing prevalence of PAD, but the awareness, treatment, and control were very low. Special attention should be paid to prevent and control PAD in China. CLINICAL TRIAL REGISTRATION NUMBER: ChiCTR-ECS-14004641.


Assuntos
Pressão Sanguínea/fisiologia , Doença Arterial Periférica/epidemiologia , Vigilância da População , Medição de Risco/métodos , Adulto , Idoso , Índice Tornozelo-Braço , China/epidemiologia , Feminino , Seguimentos , Humanos , Hipertensão/epidemiologia , Hipertensão/etiologia , Hipertensão/fisiopatologia , Masculino , Pessoa de Meia-Idade , Doença Arterial Periférica/complicações , Doença Arterial Periférica/fisiopatologia , Prevalência , Estudos Retrospectivos , Fatores de Risco
15.
Molecules ; 23(12)2018 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-30545108

RESUMO

A new triterpenoid saponin, named oleiferasaponin A2, was isolated and identified from Camellia oleifera defatted seeds. Oleiferasaponin A2 exhibited anti-hyperlipidemic activity on HepG2 cell lines. Further study of the hypolipidemic mechanism showed that oleiferasaponin A2 inhibited fatty acid synthesis by significantly down-regulating the expression of SREBP-1c, FAS and FAS protein, while dramatically promoting fatty acid ß-oxidation by up-regulating the expression of ACOX-1, CPT-1 and ACOX-1 protein. Our results demonstrate that the oleiferasaponin A2 possesses potential medicinal value for hyperlipidemia treatment.


Assuntos
Camellia/química , Metabolismo dos Lipídeos/efeitos dos fármacos , Saponinas , Ácidos Graxos/metabolismo , Células Hep G2 , Humanos , Neoplasias Hepáticas/metabolismo , Saponinas/química , Saponinas/farmacologia , Sementes/química
16.
Kidney Blood Press Res ; 43(4): 1375-1387, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30153665

RESUMO

BACKGROUND/AIMS: To investigate whether the invasively obtained central aortic systolic blood pressure (CSBP) predicts chronic kidney disease (CKD) better than brachial systolic blood pressure (SBP), brachial diastolic blood pressure (DBP) and brachial pulse pressure (PP) in the middle-aged Chinese population. METHODS: A cross-sectional study was carried out across China in 2009-2010 among the subjects aged 35-64 years. CSBP was measured non-invasively by radial artery applanation tonometry B-pro (A-PULSE CASP and corresponding software). CSBP, SBP, DBP and PP were standardized with Z-score and the odds ratios were calculated with multivariable logistic regression model. RESULTS: Data of 10197 participants were analyzed. The multivariable logistic regression after adjusting for possible confounders showed that a 1-standard deviation increment in each blood pressure measurement was associated with greater risk of CKD in both men and women (P < 0.05). The association of CSBP with CKD was stronger than SBP, DBP and PP in women, while in men the association of CSBP with CKD was stronger only than PP. With CSBP and SBP entering into the multivariable logistic regression models jointly, the odds ratio (95% confidence interval) for CSBP and SBP was 1.57 (1.39-1.79) and 1.22 (1.07-1.38) in women and 1.20 (1.03-1.39) and 1.48 (1.28-1.72) in men, respectively. With CSBP and DBP entering into the multivariable logistic regression models jointly, the odds ratio (95% confidence interval) for CSBP and DBP was 1.68 (1.52-1.84) and 1.15 (1.04-1.27) in women and 1.30 (1.15-1.46) and 1.45 (1.29-1.63) in men, respectively. With CSBP and PP entering into the multivariable logistic regression models jointly, the odds ratio (95% confidence interval) for CSBP and PP was 1.75 (1.58-1.94) and 1.06 (0.96-1.17) in women and 1.58 (1.41-1.77) and 1.04 (0.93-1.17) in men, respectively. CONCLUSION: CSBP and brachial blood pressure measurements are all predictors of CKD, however the non-invasively obtained CSBP may offer advantages over brachial blood pressure measurements in CKD risk prediction in women.


Assuntos
Aorta/fisiologia , Determinação da Pressão Arterial/métodos , Artéria Braquial/fisiologia , Insuficiência Renal Crônica/diagnóstico , Adulto , Determinação da Pressão Arterial/normas , China , Estudos Transversais , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Insuficiência Renal Crônica/fisiopatologia , Medição de Risco , Fatores Sexuais
17.
Am J Cardiol ; 122(5): 793-798, 2018 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-30049467

RESUMO

Atrial fibrillation (AF) is the most common type of arrhythmia, which is a significant public health issue. We aimed to determine the disease burden of AF in China. We used a recent national survey with stratified multistage random sampling from general Chinese population to estimate the AF disease burden, which enrolled 34,994 people aged 35 years or older all over China. The AF patients were detected by electrocardiogram during the survey or medical history taking. The quality of life in AF patients was assessed by a questionnaire similar to EQ-5D to estimate the weight of disability. Disability-adjusted life years was then calculated by adding years of life lost to years lived with disability. Overall, 31,230 samples were included in the final analysis, the weighted prevalence of AF in the Chinese population aged 35 years or older was 0.71%. Thirty-four percent of AF patients were newly diagnosed in the survey and unaware of their pathological condition beforehand. The prevalence of AF significantly increased with age. The health-related quality of life was impaired in Chinese AF patients with an overall utility value of 0.53. The total disability-adjusted life years of Chinese AF patient is estimated to be 665,400. In conclusion, the disease burden of AF in the general Chinese population was a significant public health issue in China.


Assuntos
Fibrilação Atrial/epidemiologia , Adulto , Idoso , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Inquéritos e Questionários
18.
Artigo em Inglês | MEDLINE | ID: mdl-29580941

RESUMO

BACKGROUND: Previous preclinical evidence has suggested that the elevation of epoxyeicosatrienoic acids (EETs) derived from the cytochrome P450 (CYP) epoxygenases-dependent metabolism of arachidonic acid has important anti-inflammatory effects. However, the levels of EETs and their synthetic and metabolic enzymes in human ulcerative colitis has not been evaluated. METHOD: To evaluate EETs and the expression of relevant CYP isoforms and the metabolizing enzyme, soluble epoxide hydrolase (sEH), tissue biopsies were collected from 16 pairs of ulcerative colitis patients' tissues and matched with adjacent non-inflamed tissues. EETs were extracted from tissue homogenates and analyzed by liquid chromatography coupled with tandem mass spectrometry. RESULTS: The concentration of EETs was higher in ulcerative colitis tissues compared with matched adjacent non-inflamed tissues (1.91 ±â€¯0.98 ng/mg vs. 0.96 ±â€¯0.77 ng/mg, mean ±â€¯SD, P < 0.01). As shown by immunohistochemistry, sEH was present in the cytoplasm and intestinal mucosa and showed a decline in ulcerative colitis tissues compared with matched adjacent non-inflamed tissues. Western blot analyses showed reduced sEH expression in ulcerative colitis tissues compared with matched adjacent non-inflamed tissues, whereas CYP2J2 increased in ulcerative colitis tissues (P < 0.05). However, there was no statistically significant difference observed in CYP2C8 and CYP2C9 protein expression between them (P > 0.05). CONCLUSION: Our data suggest that the increase in EET levels may be part of a protective mechanism in ulcerative colitis. Furthermore, the concentration of EETs could be a key factor for drug therapy for ulcerative colitis.


Assuntos
Ácido 8,11,14-Eicosatrienoico/metabolismo , Colite Ulcerativa/metabolismo , Sistema Enzimático do Citocromo P-450/biossíntese , Epóxido Hidrolases/biossíntese , Regulação Enzimológica da Expressão Gênica , Adulto , Idoso , Colite Ulcerativa/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
19.
Clin Chim Acta ; 481: 212-217, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29574005

RESUMO

BACKGROUND: Living at high altitude may have undesirable effects on the kidney. We explored the chronic kidney disease (CKD) prevalence and risk factors among the residents living at different altitude in Tibetan Plateau. METHODS: A cross-sectional study was carried out in 2014 to 2016 in Linzhi (2900 m altitude), Lhasa (3650 m) and Anduo (4700 m). Information on the cardiovascular risk factors was collected and blood and urine samples were measured. RESULTS: The data of 1707 subjects aged ≥35 y were analyzed. The age-standardized prevalence of CKD in Linzhi, Lhasa and Anduo was 27.7% (95% CI: 22.1-33.3%), 18.3% (12.7-24.0%) and 30.4% (23.5-37.3%) in men and 37.7% (31.8-43.6%), 29.5% (24.6-34.4%) and 36.7% (29.0-44.4%) in women, respectively. Multivariable logistic regression showed that age, female gender, systolic blood pressure, fasting serum glucose, with primary school education or lower were associated with higher risk of CKD and living in Lhasa was associated with lower risk of CKD. CONCLUSION: A higher prevalence of CKD was found in the residents living in the Tibetan Plateau. However, for the highlanders living at higher altitude does not mean higher risk. The CKD risk factors found in this study are similar to those in other studies.


Assuntos
Altitude , Insuficiência Renal Crônica/sangue , Insuficiência Renal Crônica/epidemiologia , Glicemia/análise , Pressão Sanguínea , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Fatores de Risco , Tibet/epidemiologia
20.
BMC Nephrol ; 19(1): 40, 2018 02 17.
Artigo em Inglês | MEDLINE | ID: mdl-29454330

RESUMO

BACKGROUND: The aim of the study was to assess the association between chronic kidney disease (CKD) and obesity in predicting CKD among Chinese adults, distinguishing between 5 different adiposity indices: visceral fat index (VFI), percentage body fat (PBF), body mass index (BMI), waist circumference (WC) and waist-to-height ratio (WHtR). METHODS: A total of 29,516 participants aged 35 years or above were selected using a stratified multistage random sampling method across China during 2012-2015. CKD was defined as an estimated glomerular filtration (eGFR) < 60 ml/min/1.72m2. RESULTS: The overall weighted prevalence of CKD was 3.94% (3.62% in males and 4.25% in females). All five adiposity indices had significant negative correlations to eGFR (P < 0.05). The area under the ROC (receiver operating characteristic) curves (AUC) for PBF was almost significantly larger than the other adiposity indices (P < 0.001). In addition, PBF yielded the highest Youden index in identifying CKD (male: 0.15; female: 0.20). In the logistic analysis, PBF had the highest crude odds ratios (ORs) in both males (OR: 1.819, 95% CI 1.559-2.123) and females (OR: 2.268, 95% CI 1.980-2.597). After adjusted for age, smoking status, alcohol use, education level, marital status, rural vs. urban area, geographic regions, and diagnosis of hypertension, diabetes mellitus, myocardial infarction and stroke, the ORs on PBF remained significant for both genders (P < 0.05). CONCLUSIONS: Obesity is associated with an increased risk of CKD. Furthermore, PBF was a better predictor for identifying CKD than other adiposity indices (BMI, WC, WHtR, and VFI).


Assuntos
Adiposidade/fisiologia , Antropometria/métodos , Gordura Intra-Abdominal/fisiopatologia , Insuficiência Renal Crônica/epidemiologia , Insuficiência Renal Crônica/fisiopatologia , Tecido Adiposo/fisiopatologia , Adulto , Idoso , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Obesidade/diagnóstico , Obesidade/epidemiologia , Obesidade/fisiopatologia , Insuficiência Renal Crônica/diagnóstico , Circunferência da Cintura/fisiologia
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