Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 53
Filtrar
1.
Environ Monit Assess ; 193(10): 639, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34505936

RESUMO

Lower Shiwalik foothill ranges (Kandi regions) are dominated by different land use systems, varying in fertility status due to soil erosion owing to undulating topographical features. For assessment of soil nutrient status of study area, the soil sampling was conducted to investigate the variation in micronutrient status and physicochemical characteristics of soil. Long-range basis soil sampling was carried out in 11 blocks falling in the entire Shiwalik ranges of lower Himalaya under different land use systems (LUSs), viz., agriculture (AG), horticulture (HR), agri-horticulture (AH), grassland (GL), and agroforestry (AF) systems. A total of 440 soil samples were collected from two depths (0-15, 15-30 cm) for analysis. The results had reported that the soils were slightly acidic to slightly alkaline (6.61 to 8.10) in the whole range. Soil organic carbon (SOC) was found to be varying from very low to very high (2.43 to 10.43 g kg-1). DTPA-extractable Fe and Mn were found to be in dominant concentrations in the soils whereas, the deficiency of Zn was found under all existing land uses. Interestingly, Fe (r = 0.62*), Cu (r = 0.44), and Mn (r = 0.35) micronutrients were positively correlated with clay content whereas Zn (r = - 0.02) was negatively correlated. Soil quality based on principal component analysis (SQI-PCA) reported clay, SOC, and Zn are major dominating parameters to be considered of soil in Shiwalik ranges.


Assuntos
Carbono , Solo , Agricultura , Carbono/análise , Monitoramento Ambiental , Índia , Micronutrientes
3.
BMC Public Health ; 21(1): 1345, 2021 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-34233633

RESUMO

BACKGROUND: Undernutrition is a serious matter of public health concern in India. Existing studies, policies and programs focus on women and children thereby ignoring men in policymaking. This study examines the socio-economic inequality in anaemia levels among men in India and tries to decompose the factors behind it. METHODS: The fourth round of National Family Health Survey is used to fulfill the study objectives. The outcome variable of the study is men having anaemia or not. The study uses bivariate and multivariate techniques to identify the factors associated with the outcome variable. Further, concentration index and concentration curve are calculated to measure the socio-economic inequality in anaemia among men in India. RESULTS: The results indicate that majority of the socio-economic related inequality is explained by wealth quintile followed by geographical regions of India, body mass index and educational attainment. The results also emphasize that older men belong to the high-risk groups. Moreover, the likelihood of anaemia is 40% more likely among men who belonged to East region and 25%, 13% and 7 % less likely among those who belonged to Northeast, West and South region compared to those who belonged in the North region of the country. CONCLUSION: Existing policies on anaemia should include men to achieve an anaemia free India. Individual education and awareness should be encouraged to improve nutritional status.


Assuntos
Anemia , Desnutrição , Idoso , Anemia/epidemiologia , Criança , Estudos Transversais , Feminino , Humanos , Índia/epidemiologia , Masculino , Fatores Socioeconômicos
5.
Homeopathy ; 110(2): 86-93, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33567460

RESUMO

OBJECTIVE: The aim of the study was to identify indicated homeopathic remedies based on the clinical characteristics of coronavirus disease 2019 (COVID-19) patients in India. METHODS: In this retrospective, cohort study, confirmed COVID-19 patients admitted at a COVID Health Centre in New Delhi between April 29 and June 17, 2020 were given conventional and homeopathic treatment. Patients were grouped into mild, moderate or severe categories of disease. Their symptomatologic profiles were analyzed to identify indicated homeopathic medicines. RESULTS: A total of 196 COVID-19 patients were admitted. One hundred and seventy-eight patients had mild symptoms; eighteen patients had moderate symptoms; no patients with severe symptoms were included as they were referred to tertiary care centers with ventilatory support. The mean age of patients with mild symptoms was significantly lower (38.6 years; standard deviation or SD ± 15.8) compared with patients in the moderate category (66.0 years; SD ± 9.09). The most important symptoms identified were fever (43.4%), cough (47.4%), sore throat (29.6%), headache (18.4%), myalgia (17.9%), fatigue (16.8%), chest discomfort (13.8%), chills (12.6%), shortness of breath (11.2%) and loss of taste (10.2%). Twenty-eight homeopathic medicines were prescribed, the most frequently indicated being Bryonia alba (33.3%), Arsenicum album (18.1%), Pulsatilla nigricans (13.8%), Nux vomica (8%), Rhus toxicodendron (7.2%) and Gelsemium sempervirens (5.8%), in 30C potency. CONCLUSION: Data from the current study reveal that Arsenicum album, Bryonia alba, Pulsatilla nigricans, Nux vomica, Rhus toxicodendron and Gelsemium sempervirens are the most frequently indicated homeopathic medicines. A randomized controlled clinical trial based on this finding is the next step.


Assuntos
COVID-19/terapia , Fitoterapia , Adulto , Idoso , Arsenicais/uso terapêutico , Bryonia , Estudos de Coortes , Feminino , Gelsemium , Homeopatia , Humanos , Índia , Masculino , Pessoa de Meia-Idade , Extratos Vegetais/uso terapêutico , Pulsatilla , Estudos Retrospectivos , Índice de Gravidade de Doença , Strychnos nux-vomica , Toxicodendron
6.
Nutr Health ; 27(2): 191-198, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33472523

RESUMO

BACKGROUND: Despite various programmes initiated by the Government of India, the nutritional indicators are not encouraging, as several problems like undernutrition, malnutrition and anaemia - still persist in the country, especially in the Empowered Action Group (EAG) states. AIM: Because of the dearth of studies regarding anaemia among men in India, the present study aimed to determine its prevalence in this population in the EAG states and to analyse its geographical and socio-demographic determinants. METHODS: The study utilized nationally representative, cross-sectional survey data from round 4 of the National Family Health Survey conducted in 2015-16. Bivariate analysis along with binary logistic regression were performed to assess the predictors of anaemia among men in the EAG states. RESULTS: Around a quarter of the men in the EAG states suffered from anaemia. A similar high-prevalence pattern was observed across the EAG states. Wherein, Bihar and Jharkhand had the highest prevalence of anaemia while Uttarakhand showed the lowest. Age, place of residence, marital status and caste were positively associated with the likelihood of anaemia among men in the EAG states. CONCLUSIONS: Focusing on the EAG states, this study considered the severity of anaemia as a public health problem among men. Strategies to reduce the burden of anaemia among this population are needed. The government should formulate programmes targeting anaemia specifically, and improving the nutritional status among men in general in the EAG states.


Assuntos
Anemia , Anemia/epidemiologia , Anemia/etiologia , Estudos Transversais , Humanos , Índia/epidemiologia , Masculino , Prevalência , Fatores de Risco , Fatores Socioeconômicos
7.
Sci Rep ; 10(1): 20422, 2020 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-33235268

RESUMO

Sheep farming has been fundamental to many civilizations in the world and is practiced in India since antiquity. Several thousand years of adaptation to local environmental conditions and selective breeding have evolved 44 sheep breeds in India. They are paramount in terms of economic, scientific, and cultural heritage. Genetic characterization information is imperative for sustainable utilization and conservation of ovine heritage. In this study, the genetic diversity, differentiation, and structure of 11 indigenous sheep breeds from three different agro-ecological zones of India were explored with genomic microsatellite loci and mitochondrial DNA (D loop). The estimated diversity parameters indicated that populations retained high levels of genetic diversity (Na = 8.27 ± 0.17; Ho = 0.65 ± 0.01), which provides an optimistic viewpoint for their survival. However, significant inbreeding was also observed in the nine populations. Moderate genetic differentiation existed among the groups (FST = 0.129 ± 0.012), and most likely clusters existing in the dataset are seven. Phylogenetic clustering was in line with the geographical locations of sheep populations. Mitochondrial sequences revealed high haplotype diversity with the existence of maternal haplogroups A, B, and C, and signals of population expansion. Decreased genetic diversity and unique maternal lineage (C) in endangered Tibetan and Bonpala sheep breed, warrant their immediate scientific management. Overall, the quantitative data reported here on the extant variability, and genetic relationships among the Indian sheep breeds, provide critically important inputs that will be valuable for the decision-making process on their management, both for the conservation of endangered breeds, and formulation of breeding programs to check genetic erosion.


Assuntos
DNA Mitocondrial/genética , Repetições de Microssatélites , Ovinos/classificação , Animais , Animais Domésticos/classificação , Animais Domésticos/genética , Cruzamento , Evolução Molecular , Variação Genética , Índia , Filogenia , Ovinos/genética
8.
J Obstet Gynaecol ; : 1-5, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33045849

RESUMO

The objective was to determine the relationship of histological chorioamnionitis (HCA) with genital tract cultures in preterm birth. Among two hundred women recruited for the study, 100 were taken as cases with gestational age between ≥28 and <37 weeks and 100 women with gestational age >37 weeks were taken as controls. Vaginal swabs were taken for culture sensitivity and vaginal smears were made for performing whiff test and heat dry gram stained smear was examined for growth of microorganisms. Histopathologic examination of the placenta was done after delivery. 49 cases and 26 controls had evidence of histological chorioamnionitis. A significant difference was observed in relation to the presence of E. coli, presence of clue cells, positive whiff test and occurrence of bacterial vaginosis in subjects with and without histological chorioamnionitis. Thus, we conclude that the presence of histological chorioamnionitis is closely related to the presence of pathogenic microorganisms in the cervicovaginal region.IMPACT STATEMENTWhat is already known on the subject? Histologic chorioamnionitis has been regarded to reflect amniotic fluid infection and there are studies showing an association between histologic chorioamnionitis, amniotic fluid, and subchorionic plate cultures. Nevertheless, studies of the correlation of the cervical swab cultures with intrauterine infection in preterm birth remain inconclusive.What do the results of this study add? Histologic chorioamnionitis is closely related to the presence of pathogenic microorganisms in the cervicovaginal region.What are the implications of these findings for clinical practice and/or further research? High vaginal swab cultures and gram staining of vaginal smear is useful in detecting antenatal patients who are at a higher risk for preterm labour. After detection, early intervention may be done to avoid preterm deliveries in these high-risk pregnancies.

9.
BMC Pulm Med ; 20(1): 190, 2020 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-32664897

RESUMO

BACKGROUND: Asthma is one of the leading causes of disease burden when measured in terms of disability adjusted life years, despite low prevalence of self-reported cases among young women. This paper deals with the meso-scale correlates and spatial heterogeneity in the prevalence of self-reported Asthma across 640 districts in India, using a nationally representative sample of 699,686 women aged 15-49 years from all 36 States/UTs under NFHS-4 (2015-16). METHODS: Analytical methods used in this paper include multivariate logistic regression to examine the adjusted effects of various independent variables on self-reported Asthma and poor-rich ratios (PRR) and concentration index (CI) to understand the economic inequalities in the prevalence of Asthma. For the spatial analysis in the prevalence of Asthma, univariate and bivariate local Moran's I statistic have been computed in addition to measure of spatial autocorrelation and auto regression using spatial error and spatial lag models. RESULTS: Results highlight that women's education was an important marker to the prevalence of Asthma. Smoking tobacco in any form among women were significantly more likely to suffer from Asthma. The prevalence of Asthma was further aggravated among women from the households without a separate room for kitchen, as well as those using unclean fuel for cooking. The poor-rich ratio in the prevalence of Asthma across various States/UTs in India depict inherent inequality. An analysis of spatial clustering in the prevalence of Asthma based on spatial autocorrelation portrays that Moran's I values were significant for improved source of drinking water, clean fuel used for cooking, and household environment. When spatial weights are taken into consideration, the autoregression model noticeably becomes stronger in predicting the prevalence of Asthma. CONCLUSIONS: Any programmatic effort to curb the prevalence of Asthma through vertical interventions may hinge around the use of clean fuel, poverty, and lifestyle of subjects, irrespective of urban-rural place of their residence, environmental and ecological factors.


Assuntos
Asma/epidemiologia , Fatores Socioeconômicos , Análise Espacial , Adolescente , Adulto , Culinária/métodos , Culinária/estatística & dados numéricos , Características da Família , Feminino , Humanos , Índia/epidemiologia , Modelos Logísticos , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , População Rural , Autorrelato , Fumar Tabaco/efeitos adversos , Fumar Tabaco/epidemiologia , População Urbana , Adulto Jovem
10.
Mol Biol Rep ; 47(8): 6429-6434, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32666440

RESUMO

India has a rich heritage of rearing cattle where farmers selected native cattle suitable to their local agro-ecological conditions for centuries. It is reflected in 50 indigenous breeds of cattle, besides many lesser known populations not explored so far. It is the need of the hour to characterize such populations to have prudent improvement and conservation options. Thus, present study was carried out to assess the genetic diversity and relationship between an unexplored local cattle population (Kathani) and four established cattle breeds of adjoining area (Gaolao, Kosali, Ongole and Motu) by using 20 FAO recommended microsatellite markers. High variability was recorded in the Kathani population with a total of 198 alleles that varied between 5 (ILSTS11, TGLA22, INRA05) and 17 (ILSTS34) with a mean of 9.9 ± 0.73. The average observed heterozygosity (Ho) was 0.658 ± 0.054. Heterozygote deficiency was not significant (FIS = 0.029 ± 0.063) indicating random mating prevalent across this population. Mean estimates of observed number of alleles and heterozygosity over all the loci and five populations were 9.73 ± 0.421 and 0.617 ± 0.022, respectively. In the overall populations, the homozygote excess (FIT) of 0.293 ± 0.032, was partly due to the homozygote excess within breeds (FIS = 0.121 ± 0.025) and to a larger extent due to high (0.05 < FST < 0.15) genetic differentiation among them (FST = 0.195 ± 0.029). Substantial pairwise Nei's genetic distance and high population differentiation indicated towards separate genetic identity of Kathani cattle. The analysis of genetic structure based on Bayesian approach indicated that the most probable number of clusters is five confirming definitive genetic differentiation among all the popultions. Entire analysis showed that a significant amount of genetic variation is maintained in Kathani, a lesser known cattle population that is distinct from the recognized breeds in the proximity. As this autochthonous cattle plays role in the economic sustainability of a marginal and disadvantaged area, it is important to preserve and develop its breeding.


Assuntos
Bovinos/genética , Repetições de Microssatélites , Alelos , Animais , Teorema de Bayes , Cruzamento , Análise por Conglomerados , Variação Genética , Heterozigoto , Homozigoto , Índia
11.
Gene ; 756: 144910, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32574758

RESUMO

Thirty four distinct breeds and many non-descript populations represent the caprine diversity of India. Genetic characterization of breeds is an essential element in designing breeding strategies and preserving genetic diversity. Considering the popularity of mitochondrial DNA for phylogeographical studies, this study involved an extensive analysis of population structure and genetic diversity of 28 defined breeds and 5 lesser known populations representing all four major agro-climatic zones of India using mitochondrial DNA markers. Analysis of hypervariable region 1 of mtDNA control region in 443 goats together with 22 reference sequences, delineated 341 distinct haplotypes belonging to four maternal haplogroups; A, B, C and D, with haplogroup A representing 90% of the individuals. The haplotype and nucleotide diversity indices of Indian goats were 0.998 ± 0.001 and 0.028 ± 0.001, respectively indicating abundant genetic variability. Estimates of population demographic parameters from mismatch analysis suggested a relatively good fit to the model of either spatial or demographic expansion of Indian goats. AMOVA analysis and topology of MJ network suggested lack of phylogeographic structure in domestic goats, which can be attributed to unstructured animal breeding, dwindling pastures and nomadic pastoralism. Genetic differentiation between goats from different agro-ecological regions was in accordance with their geographical propinquity.


Assuntos
DNA Mitocondrial/genética , Variação Genética , Cabras/classificação , Cabras/genética , Animais , Domesticação , Genética Populacional , Haplótipos , Índia , Filogeografia
12.
Saudi J Biol Sci ; 27(7): 1699-1709, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32565685

RESUMO

Camel invokes fascinating chapter of Indian desert history and is integral component of its ecosystem. Camel population has reached a crisis point after three decades of decline (75%) causing major concern to the policy makers. >28% of Indian camel is not yet characterized. It is imperative to describe country's camel germplasm and its existing diversity for designing conservation plan. One such population is Sindhi, distributed along border with Pakistan. Twenty five microsatellite markers being valuable tool for estimating genetic diversity were selected to elucidate genetic variability and relationship of Sindhi with two registered camel breeds of India- Marwari and Kharai. The standard metrics of genomic diversity detected moderate variability in all the three populations. A total of 303 alleles with a mean of 8.116 ± 0.587 alleles per locus were found in total of 143 animals. Sindhi population had intermediate allelic diversity with 8.522 ± 1.063 alleles per locus. Corresponding values in Marwari and Kharai were 8.783 ± 0.962 and 7.043 ± 1.030, respectively. Genetic variability within the breeds was moderate as evidenced by the mean observed heterozygosity of 0.556 ± 0.025. Sindhi camel population harbors higher genetic variability (Ho = 0.594) as compared to the two registered camel breeds (Marwari, 0.543 and Kharai, 0.531). Mean expected heterozygosity under Hardy-Weinberg equilibrium was higher than the observed values across the three camel groups, indicating deviations from assumptions of this model. In fact, average positive F value of 0.084 to 0.206 reflected heterozygote deficiency in these populations. These Indian camel populations have not experienced serious demographic bottlenecks in the recent past. Differences among populations were medium and accounted for 7.3% of total genetic variability. Distinctness of three camel populations was supported by all the approaches utilized to study genetic relationships such as genetic distances, phylogenetic relationship, correspondence analysis, clustering method based on Bayesian approach and individual assignment. Sindhi camel population was clearly separated from two registered breeds of Indian camel. Results conclude Sindhi to be a separate genepool. Moderate genetic diversity provides an optimistic viewpoint for the survival of severely declining indigenous camel populations with appropriate planning strategies for conserving the existing genetic variation and to avoid any escalation of inbreeding.

13.
Environ Sci Pollut Res Int ; 27(10): 10966-10980, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31950423

RESUMO

The 4-nitrophenol (4-NP) is one of the carcinogenic pollutants listed by US EPA and has been detected in industrial wastewater. This study investigates the photocatalytic degradation of 4-NP with TiO2 and boron (B)-doped TiO2 nanostructures. The degradation on undoped and B-doped TiO2 with various boron loadings (1-7%) was studied to establish a relationship between structure, interface, and photo-catalytic properties. The results of XRD, micro Raman, FTIR, and HRTEM show that the B doping has improved the crystallinity and induces rutile phase along with anatase (major phase). The N2 adsorption-desorption, SEM-EDX, and XPS indicated that the B induced the formation of mesoporous nanostructures in TiO2 and occupies interstitial sites by forming Ti-O-B type linkage. The surface area of pure TiO2 was decreased from 235.4 to 63.3 m2/g in B-TiO2. The photo-physical properties were characterized by UV-Vis DRS, which showed decrease in the optical band-gap of pure TiO2 (2.98 eV) to B-TiO2 (2.95 eV). The degradation results demonstrated that the B doping improved the photocatalytic activity of TiO2; however, this improvement depends on the B concentration in doped TiO2. B-doped TiO2 (> 5% B) showed 90 % degradation of 4-NP, whereas the undoped TiO2 can degrade only 79 % of 4-NP. The degradation followed pseudo-first-order kinetics with rate constant values of 0.006 min-1 and 0.0322 min-1 for pure TiO2 and B-TiO2 respectively. The existence of a reduced form of Ti3+ on the surface of TiO2 (as evidence from XPS) was found responsible for enhancement in photocatalytic activity.


Assuntos
Nanoestruturas , Titânio , Catálise , Cinética , Nitrofenóis , Fotólise
14.
Food Addit Contam Part B Surveill ; 13(1): 59-63, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31766982

RESUMO

Aflatoxin M1 (AFM1) contamination in 150 samples of milk, sold in market of Hisar city of Haryana, India, was investigated by using High Pressure Liquid Chromatography (HPLC). Out of these, 40 samples contained AFM1 at a concentration below the limit of detection (LOD) of 0.052 µg/kg. Among the AFM1 contaminated samples, 46 raw milk samples contained a concentration above the LOD but less than the limit of quantitation (LOQ), whereas 64 samples were above the LOQ. Of these samples, 31 exceeded the maximum limit of 0.5 µg/kg prescribed by FSSAI, India. Based on this study, the dietary intake of AFM1 for adults through consumption of milk was estimated. The results indicated that AFM1 contamination can be a food safety issue for raw and pasteurised milk consumed in India. Therefore, there is a need for a national monitoring programme to control the level of mycotoxins in milk.


Assuntos
Aflatoxina M1/análise , Exposição Dietética , Contaminação de Alimentos/análise , Leite/química , Adulto , Animais , Humanos , Índia , Medição de Risco
15.
Contemp Clin Dent ; 10(4): 668-671, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-32792828

RESUMO

The importance of pink esthetics as well as increased patient comfort has been gaining its pace in dentistry. Moreover, when it comes to the treatment of gingival recession, the ideologies of the periodontists have changed over time, i.e., from "extension to prevention;" it has now become "conserve to preserve." Utilizing this same principle, pinhole technique was introduced comprising sutureless surgery with minimal surgical intervention, thus providing maximum comfort to the patient while undergoing treatment and postoperatively. This article presents a case report of two cases who were treated with a minimally invasive pinhole surgical technique, with complete root coverage and minimal complications.

16.
J Dairy Res ; 86(1): 98-101, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30520408

RESUMO

The aim of this Research Communication was to contribute to the knowledge of milk sialic acid concentration of bovines with specific focus on India. Sialic acids (SA) are important constituents of mammalian milks. Buffaloes are the main milk producing species in India, therefore, our research focused on both cow and buffalo. Two Indian cattle (Bos indicus) breeds (Sahiwal, Tharparkar), one cross bred cattle - Karan Fries (Tharparkar × Holstein Friesian) and a buffalo breed (Murrah) were selected. Systematic comparisons of the total, free and bound form of SA and also its distribution over the course of lactation- colostrums and mature milk (120-140 d) was generated. Animal management, sample collection and methodology of SA estimation were identical for the different groups. Colostrum had the highest concentration of SA, which declined with the progress of lactation in all the groups. Majority of the SA existed in bound form. No significant (P < 0.05) difference was recorded in the total, bound or free SA across all the groups. However, differences were obvious in the total and bound SA level in the mature milk. Indian cattle, Sahiwal and Tharparkar were equivalent, but had higher concentration of total and bound SA than crossbred cattle. Milk of buffalo had SA equivalent to that of crossbred cattle. The mean (se) levels of total SA was 23.4 (0.8), 25.8 (2.4), 20.3 (0.6) and 20.2 (1.2) in Sahiwal, Tharparkar, cross bred and Murrah buffalo, respectively. The findings suggested that milk of indigenous cattle may be a potential source of SA, a bioactive compound with beneficial effect on human health and a potential functional ingredient in foods. Results add value to the currently declining indigenous cattle of India.


Assuntos
Búfalos , Bovinos , Lactação/fisiologia , Leite/química , Ácido N-Acetilneuramínico/análise , Animais , Biodiversidade , Cruzamento/métodos , Búfalos/genética , Bovinos/genética , Colostro/química , Cruzamentos Genéticos , Feminino , Índia , Valor Nutritivo , Especificidade da Espécie
17.
Nucleic Acid Ther ; 28(6): 357-365, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30457923

RESUMO

Artificial microRNA (amiRNA)-mediated inhibition of viral replication has recently gained importance as a strategy for antiviral therapy. In this study, we evaluated the benefit of using the amiRNA vector against Japanese encephalitis virus (JEV). We designed three single amiRNA sequences against the consensus sequence of 3' untranslated region (3'UTR) of JEV and tested their efficacy against cell culture-grown JEV Vellore strain (P20778) in neuronal cells. The binding ability of three amiRNAs on 3'UTR region was tested in vitro in HEK293T cells using a JEV 3'UTR tagged with luciferase reporter vector. Transient transfection of amiRNAs was nontoxic to cells as evident from the MTT assay and caused minimal induction in interferon-stimulated gene expression. Furthermore, our result suggested that transient expression of two amiRNAs (amiRNA #1 and amiRNA #2) significantly reduced intracellular viral RNA and nonstructural 1 (NS1) protein, as well as diminished infectious viral particle release up to 95% in the culture supernatant as evident from viral plaque reduction assay. Overall, our results indicated that RNA interference based on amiRNAs targeting viral conserved regions at 3'UTR was a useful approach for improvements of nucleic acid inhibitors against JEV.


Assuntos
Vírus da Encefalite Japonesa (Espécie)/genética , Encefalite Japonesa/genética , MicroRNAs/farmacologia , Replicação Viral/genética , Vírus da Encefalite Japonesa (Espécie)/patogenicidade , Encefalite Japonesa/terapia , Encefalite Japonesa/virologia , Células HEK293 , Humanos , MicroRNAs/genética , Neurônios/patologia , Neurônios/virologia , Interferência de RNA , Transfecção
18.
Sci Rep ; 8(1): 12717, 2018 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-30143719

RESUMO

Microwell platforms show great promise in single-cell studies and protein measurements because of their low volume sampling, rapid analysis and high throughput screening ability. However, the existing actuation mechanisms to manipulate the target samples and fabrication procedures involved in the microwell-based microfluidic devices are complex, resource-intensive and require an external power source. In this work, we present proof of concept of a simple, power-free and low-cost closed magnet digital microfluidics device for isolating biological entities in femtoliter-sized microwells. The target biological entities were encapsulated in magnetic liquid marbles and shuttled back and forth between micropatterned top and bottom plates in the microdevice to obtain high loading efficiency and short processing time. The microdevice performance was studied through fluorescent detection of three different entities: microbeads, bovine serum albumin (BSA) and Escherichia coli, captured in the microwell array. Almost 80% of the microwells were loaded with single microbeads in five shuttling cycles, in less than a minute. Further, a low volume of BSA was compartmentalized in the microwell array over a two order range of concentration. The microdevice exhibits two unique features: lotus leaf stamps were used to fabricate micropatterns (microwells and micropillars) on top and bottom plates to impart functionality and cost-effectiveness, and the target samples were actuated by a permanent magnet to make the microdevice power-free and simple in operation. The developed biomimetic microdevice is therefore capable of capturing a multitude of biological entities in low-resource settings.


Assuntos
Materiais Biomiméticos , Biomimética/instrumentação , Imãs , Microfluídica/instrumentação , Animais , Bovinos , Escherichia coli/metabolismo , Fluoresceína-5-Isotiocianato/análogos & derivados , Fluoresceína-5-Isotiocianato/metabolismo , Fluorescência , Interações Hidrofóbicas e Hidrofílicas , Nanopartículas/ultraestrutura , Soroalbumina Bovina/metabolismo , Processamento de Sinais Assistido por Computador , Molhabilidade
19.
J Midlife Health ; 9(2): 106-109, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29962811

RESUMO

One of the major manifestations of methimazole-induced neutropenia or agranulocytosis is diffuse gingival ulceration and necrosis. The aim of this case report is to be familiar with drug-induced neutropenia and its associated oral manifestations and the healing of the gingiva after proper treatment.

20.
Case Rep Gastroenterol ; 12(1): 1-6, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29515338

RESUMO

Duodenal perforation is a rare but life-threatening complication of endoscopic retrograde cholangiopancreatography (ERCP). Duodenal perforation can cause air leak into the extraperitoneal space. In rare instances, the air in the extraperitoneal space could dissect along the fascial planes of the abdomen to reach scrotum, leading to pneumoscrotum. We present the case of a 35-year-old male patient who developed scrotal pain and swelling following ERCP. He was found to have extensive pneumoscrotum, pneumoretroperitoneum, pneumomediastinum, and subcutaneous emphysema. The patient was diagnosed with retroperitoneal duodenal perforation. He was managed conservatively with close monitoring and supportive care.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...