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1.
J Family Med Prim Care ; 11(5): 2106-2113, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35800574

RESUMO

Objective: To study the epidemiological characteristics of the pandemic by describing the clinical profile of the COVID-19 patients presenting to a super specialty hospital. Methods: This was a descriptive study using medical records of patients who tested positive for SARS-CoV-2 RNA using reverse transcription-polymerase chain reaction between 17th March and 15th January 2021 while maintaining confidentiality. The clinical and demographic data of all the patients were entered in a Microsoft Excel and statistical analysis was done using SPSS 21 software. Regression analysis was performed and a P value < 0.05 was considered to be statistically significant. Results: A total of 3534 patients were enrolled in this study aged 9-96 years. Among patients with symptoms, fever and cough were the most common presenting symptoms, while 5.6% of the patients were asymptomatic. Hypertension was the most common comorbidity (37%), while no comorbidities were present in 43.0% of the participants and this was statistically significant for age (P = 0.000). Among patient outcomes, >50% of patients were in home isolation, while 11% of patients had a fatal outcome. Elder age group had a higher proportion of expiry among outcomes (P <= 0.001). Most patients had a hospital stay of 9-11 days. A total of 63 health workers were included with male: female ratio being 3.5:1. Conclusion: Our study reflects that majority of the positive cases that presented to the hospital had mild/moderate symptoms. We believe that appropriate triaging of patients followed by early institution of medicine and good critical care services may help to control this epidemic.

2.
Sci Rep ; 12(1): 11976, 2022 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-35831356

RESUMO

Borderline personality disorder (BoPD or BPD) is highly prevalent and characterized by reactive moods, impulsivity, behavioral dysregulation, and distorted self-image. Yet the BoPD diagnosis is underutilized and patients with BoPD are frequently misdiagnosed resulting in lost opportunities for appropriate treatment. Automated screening of electronic health records (EHRs) is one potential strategy to help identify possible BoPD patients who are otherwise undiagnosed. We present the development and analytical validation of a BoPD screening algorithm based on routinely collected and structured EHRs. This algorithm integrates rule-based selection and machine learning (ML) in a two-step framework by first selecting potential patients based on the presence of comorbidities and characteristics commonly associated with BoPD, and then predicting whether the patients most likely have BoPD. Leveraging a large-scale US-based de-identified EHR database and our clinical expert's rating of two random samples of patient EHRs, results show that our screening algorithm has a high consistency with our clinical expert's ratings, with area under the receiver operating characteristic (AUROC) 0.837 [95% confidence interval (CI) 0.778-0.892], positive predictive value 0.717 (95% CI 0.583-0.836), accuracy 0.820 (95% CI 0.768-0.873), sensitivity 0.541 (95% CI 0.417-0.667) and specificity 0.922 (95% CI 0.880-0.960). Our aim is, to provide an additional resource to facilitate clinical decision making and promote the development of digital medicine.


Assuntos
Transtorno da Personalidade Borderline , Registros Eletrônicos de Saúde , Algoritmos , Transtorno da Personalidade Borderline/diagnóstico , Transtorno da Personalidade Borderline/epidemiologia , Bases de Dados Factuais , Humanos , Aprendizado de Máquina
3.
Cureus ; 14(5): e25377, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35774643

RESUMO

CONTEXT: Patella fracture is frequently encountered in day to day practice of every orthopedic surgeon, yet conventional modalities of fixation that are associated with a high incidence of implant removal continue to be standard practice. AIM: To estimate the incidence of implant removal in surgically managed patella fractures. SETTINGS AND DESIGN: Retrospective study. MATERIALS AND METHODS: Data of surgically managed patella fractures in our institution from January 2017 to December 2019 was collected from the hospital management system and analyzed retrospectively. Statistical Package for Social Sciences (SPSS) version 23 (IBM Corp., Armonk, NY, USA) was used for the analysis of data. Nominal and categorical variables were analyzed using Chi-square, and continuous data were expressed as mean and standard deviation. A p-value ≤0.05 was considered significant. RESULTS: The study group had a total of 106 patients, with 74 males and 32 females and an average age of 47.67 ± 17.85 years. Most commonly the fractures occurred following an accidental fall and road traffic accident. A comminuted pattern was most common. The mean length of stay was 7.34 ± 4.21 days. Osteosynthesis with Kirschner wire and tension band wires was done in 83 cases. The average follow-up was 13 months (range, three to 48 months). Twenty-two patients (one in five) required a second surgery for implant removal and the rate was higher in patients more than 60 years of age, females, and comminuted fractures. Cases managed with cannulated cancellous screws did not require any removal. The average timing of removal was 14 months (range, seven to 28 months). Relief of symptoms following removal was observed in 86% of cases. CONCLUSION: Conventional fixation techniques offer good union rates but at a cost of a high incidence of removal. Multi-centric trials are required to compare the rates of removal of conventional methods with new techniques being adopted.

4.
Chem Biol Drug Des ; 2022 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-35822451

RESUMO

Cancer is one of the most prevailing disease conditions, which occurs due to uncontrolled cell division either due to natural mutation to the genes or due to changes induced by physical, chemical, or biological carcinogens. According to WHO, it is the second leading cause of death worldwide and has reported 10 million deaths in 2020. Hence, there arises the need for better chemotherapies and DNA intercalators are one such emerging therapy for cancer. DNA intercalating agents reversibly intercalate with the double-helical structure of DNA by interacting with adjacent base pairs and disrupting the structure of DNA and thereby causing cell death. Here, we discuss the different classes of organo-intercalators used in cancer therapy describing their anticancer and intercalation ability by different methods along with their structure-activity relationship and mechanism of action.

5.
Curr Med Chem ; 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35894454

RESUMO

Recently released Globocan-2020 report has been disclosed an increase in new cancer cases, cancer deaths, and 5-year prevalence cases worldwide. The higher percent proportions of cancer deaths as compared to their incidence percentage in Asia and Africa. Cancer is a genetic but not inheritable disease that consists of various abnormal cells. Depending upon the nature and site of availability of cells cancer can spread all over the body. These abnormal cells can grow infinitely in which tyrosine kinases (TKs) play an important role as mediators for cellular signal transduction processes during migration, metabolism, proliferation and differentiation, apoptotic cell death, etc. TKs belong to a specific family of an enzyme that catalyses the transferring of phosphate groups from ATP to selected tyrosine residues of a target protein during the biological process to maintain the homeostasis. They work in various steps of development and progression pathways of cancer by affecting signal transduction. The aberrant and deregulated functioning of TKs results in a defective signal transduction pathway which leads to abnormality in cell transformation, proliferation, and differentiation, thus the development of cancer. Since their discovery in 1990 to date, more than 90 TKs have been reported and divided into two categories receptor and non-receptor TKs. Higher expression levels of TKs paved their status of oncoprotein and thus, they provide a potential target for the development of anti-cancer therapeutics. Here, we provided updated cancer demographic status, cancer types, and available therapeutic options targeted cancer therapeutic strategies and the role of different TKs in cancers along with recently identified molecules that target TKs. Moreover, we also included the binding interactions of chemical inhibitors with TKs.

6.
Pharmaceutics ; 14(7)2022 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-35890297

RESUMO

The present study aims to design, develop and characterize kNGR (Asn-Gly-Arg) peptide-conjugated lipid-polymer-based nanoparticles for the target-specific delivery of anticancer bioactive(s), i.e., Paclitaxel (PTX). The kNGR-PEG-DSPE conjugate was synthesized and characterized by using spectral analysis. The dual-targeted PLGA-lecithin-PEG core-shell nanoparticles (PLNs-kNGR-NPs) were synthesized using a modified nanoprecipitation process, and their physiological properties were determined. The results support that, compared to other NPs, PLNs-kNGR-NPs are highly cytotoxic, owing to higher apoptosis and intracellular uptake. The significance of rational nanoparticle design for synergistic treatment is shown by the higher tumor volume inhibition percentage rate (59.7%), compared to other designed formulations in Balb/c mice in the HT-1080 tumor-induced model. The overall results indicate that the PLNs-kNGR-NPs-based hybrid lipid-polymer nanoparticles present the highest therapeutic efficacy against solid tumor overexpressing the CD13 receptors.

7.
Viruses ; 14(7)2022 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-35891434

RESUMO

The genus of Xanthomonas contains many well-known plant pathogens with the ability to infect some of the most important crop plants, thereby causing significant economic damage. Unfortunately, classical pest-control strategies are neither particularly efficient nor sustainable and we are, therefore, in demand of alternatives. Here, we present the isolation and characterization of seven novel phages infecting the plant-pathogenic species Xanthomonas translucens and Xanthomonas campestris. Transmission electron microscopy revealed that all phages show a siphovirion morphology. The analysis of genome sequences and plaque morphologies are in agreement with a lytic lifestyle of the phages making them suitable candidates for biocontrol. Moreover, three of the isolated phages form the new genus "Shirevirus". All seven phages belong to four distinct clusters underpinning their phylogenetic diversity. Altogether, this study presents the first characterized isolates for the plant pathogen X. translucens and expands the number of available phages for plant biocontrol.


Assuntos
Bacteriófagos , Xanthomonas , Bacteriófagos/genética , Filogenia
8.
Mol Neurobiol ; 59(8): 5149-5167, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35674862

RESUMO

Histological interpretation of the rare pleomorphic xanthoastrocytoma (PXA) has been the holy grail for treatment options. However, no stand-alone clinical interventions have been developed owing to the lack of gene expression profiling data in PXA/APXA patients. We first time report the comprehensive analyses of the coding as well as long non-coding RNA (lncRNA) signatures of PXA/APXA patients. Several genes such as IGFBP2, NF1, FOS, ERBB2, and lncRNAs such as NEAT1, HOTAIRM1, and GAS5 known to play crucial roles in glioma patients were also deregulated in PXA patients suggesting the commonality in the molecular signatures. PPI network, co-expression, and lncRNA-mRNA interaction studies unraveled hub genes (such as ERBB2, FOS, RPA1) and networks that may play a critical role in PXA biology. The most enriched pathways based on gene profiles were related to TLR, chemokine, MAPK, Rb, and PI3K-Akt signaling pathways. The lncRNA targets were enriched in glucuronidation, adipogenesis, TGF-beta signaling, EGF/EGFR signaling, and cell cycle pathways. Interestingly, several mRNAs like PARVG, and ABI2 were found to be targeted by multiple lncRNAs suggesting a tight control of their levels. Some of the most prominent lncRNA-mRNA pairs were LOC728730: MRPL9, XLOC_l2_011987: ASIC2, lnc-C1QTNF5-1: RNF26. Notably, several lncRNAs such as lnc-CETP-1, lnc-XRCC3-1, lnc-RPL31-1, lnc-USP13-1, and MAPKAPK5-AS1, and genes such as RPA1, NTRK3, and CNRP1 showed strong correlation to the progression-free survival of PXA patients suggesting their potential as novel biomarkers. Overall, the findings of this study may facilitate the development of a new realm of RNA biology in PXA that may have clinical significance in the future.


Assuntos
Astrocitoma , RNA Longo não Codificante , Astrocitoma/genética , Perfilação da Expressão Gênica , Redes Reguladoras de Genes , Humanos , Proteínas de Neoplasias/genética , Fosfatidilinositol 3-Quinases/genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteases Específicas de Ubiquitina
9.
Lancet Reg Health Southeast Asia ; 3: 100023, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35769163

RESUMO

Background: Surge of SARS CoV-2 infections ascribed to omicron variant began in December 2021 in New Delhi. We determined the infection and reinfection density in a cohort of health care workers (HCWs) along with vaccine effectiveness (VE) against symptomatic infection within omicron transmission period (considered from December 01, 2021 to February 25, 2022. Methods: This is an observational study from the All India Institute of Medical Sciences, New Delhi. Data were collected telephonically. Person-time at risk was counted from November 30, 2021 till date of infection/ reinfection, or date of interview. Comparison of clinical features and severity was done with previous pandemic periods. VE was estimated using test-negative case-control design [matched pairs (for age and sex)]. Vaccination status was compared and adjusted odds ratios (OR) were computed by conditional logistic regression. VE was estimated as (1-adjusted OR)X100-. Findings: 11474 HCWs participated in this study. The mean age was 36⋅2 (±10⋅7) years. Complete vaccination with two doses were reported by 9522 (83%) HCWs [8394 (88%) Covaxin and 1072 Covishield (11%)]. The incidence density of all infections and reinfection during the omicron transmission period was 34⋅8 [95% Confidence Interval (CI): 33⋅5-36⋅2] and 45⋅6 [95% CI: 42⋅9-48⋅5] per 10000 person days respectively. The infection was milder as compared to previous periods. VE was 52⋅5% (95% CI: 3⋅9-76⋅5, p = 0⋅036) for those who were tested within 14-60 days of receiving second dose and beyond this period (61-180 days), modest effect was observed. Interpretation: Almost one-fifth of HCWs were infected with SARS CoV-2 during omicron transmission period, with predominant mild spectrum of COVID-19 disease. Waning effects of vaccine protection were noted with increase in time intervals since vaccination. Funding: None.

10.
J Appl Stat ; 49(2): 371-393, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35707212

RESUMO

This article introduces a two-parameter exponentiated Teissier distribution. It is the main advantage of the distribution to have increasing, decreasing and bathtub shapes for its hazard rate function. The expressions of the ordinary moments, identifiability, quantiles, moments of order statistics, mean residual life function and entropy measure are derived. The skewness and kurtosis of the distribution are explored using the quantiles. In order to study two independent random variables, stress-strength reliability and stochastic orderings are discussed. Estimators based on likelihood, least squares, weighted least squares and product spacings are constructed for estimating the unknown parameters of the distribution. An algorithm is presented for random sample generation from the distribution. Simulation experiments are conducted to compare the performances of the considered estimators of the parameters and percentiles. Three sets of real data are fitted by using the proposed distribution over the competing distributions.

11.
Indian J Psychol Med ; 44(1): 17-21, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35509647

RESUMO

Background: Suicide results from complex interactions of various risk factors-reasons for dying (RFD)-and protective factors-reasons for living (RFL). Suicide is not necessarily a wish to die but may be an appeal for help. We analyzed RFD and RFL in persons who had attempted suicide, through their clinical records at a Crisis Intervention Clinic (CIC). Methods: We retrospectively analyzed demographic and clinical data, and classified RFD and RFL, among patients with either ideas or attempt of suicide registered at our CIC (N = 83). Using two open-ended questions from the clinical history data, we derived their RFD or RFL; (n = 53) completed these questions regarding RFD-RFL. Results: In the total sample, males and females were equally represented and educated, but males were significantly older. Most common diagnosis was nonpsychotic mood disorder. Commonest mode of suicide attempt was hanging. Family conflict vs. family responsibility, hope vs. hopelessness, stressful life events, and negative cognitions about the self and the world were important RFD. RFL included feeling responsible, love for family and for self, hope, career success, and religious beliefs. Conclusion: RFD and RFL could both be grouped in similar categories related to family, career, hope, etc.

12.
Neurol India ; 70(2): 704-709, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35532643

RESUMO

Background: Idiopathic intracranial hypertension (IIH) is well-described entity in literature. However, large studies on clinical and radiological profile are still very few from the Indian subcontinent. Aims: To analyze the clinical and radiological profile of IIH and correlation of various clinical and radiological parameters with papilledema and CSF opening pressure. Setting and Design: Hospital-based observational, descriptive study. Materials and Methods: This study analyzed 122 patients admitted in the department of Neurology (from January 2014 to December 2018) for detailed history, clinical examination, and neuroimaging. CSF manometry and routine CSF analysis were done. All participants met the modified Dandy criteria. Patients with secondary causes of raised ICP and primary ocular pathology were excluded. Statistical Analysis Used: Descriptive statistics and Chi-square test. Results: Among 122 cases 86 (70.49%) were females. The mean age was 33 years. 62 (50.82%) cases were obese. Headache was the most common symptom (114/122; 93.44%). Bilateral papilledema was noted in 114 (93.44%) cases. Higher grades of papilledema had a correlation with higher CSF opening pressure. Empty sella was the most common radiological finding (100/122; 81.97%). All radiological findings except empty sella had a correlation with higher CSF opening pressure. Conclusion: IIH typically affects obese females of childbearing age group. However, it is not uncommon in the nonobese and male sex. Obesity may not be a dominant risk factor for the development of IIH in the Indian subcontinent. Cases with normal BMI and male sex with clinical features of raised intracranial pressure and normal neuroimaging should also be evaluated for IIH. Cases with refractory headache and empty sella on MRI should be evaluated for IIH.


Assuntos
Hipertensão Intracraniana , Papiledema , Pseudotumor Cerebral , Adulto , Feminino , Cefaleia/complicações , Cefaleia/etiologia , Humanos , Índia/epidemiologia , Hipertensão Intracraniana/complicações , Hipertensão Intracraniana/diagnóstico por imagem , Masculino , Obesidade/complicações , Papiledema/diagnóstico por imagem , Papiledema/etiologia , Pseudotumor Cerebral/complicações , Pseudotumor Cerebral/diagnóstico por imagem , Centros de Atenção Terciária
13.
Front Oncol ; 12: 824594, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35402240

RESUMO

DNA methylation, catalyzed by DNA methyltransferase (DNMT), is a well-characterized epigenetic modification in cancer cells. In particular, promoter hypermethylation of AR and ESR1 results in loss of expression on Androgen Receptor (AR) and Estrogen Receptor (ER), respectively, and is associated with a hormone refractory state. We now report that Glycogen Synthase Kinase 3 (GSK3) phosphorylates DNMT1 at S714, which is localized to a 62 amino acid region referred to as auto-inhibitory linker, which functions to occlude the DNA from the active site of DNMT1 to prevent the methylation of unmethylated DNA. Molecular Dynamics simulation indicates that phosphorylation at S714 resulted in conformational rearrangement of the autoinhibitory domain that inactivated its ability to block the methylation of unmethylated DNA and resulted in enhanced DNA binding. Treatment with a novel and more selective inhibitor of GSK3 resulted in decreased methylation of the promoter region of genes encoding the Androgen Receptor (AR) and Estrogen Receptor alpha (ERa) and re-expression of the AR and ERa in AR negative prostate cancer and ER negative breast cancer cells, respectively. As a result, concurrent treatment with the GSK3 inhibitor resulted in responsiveness of AR negative prostate cancer and ER negative breast cancer cells to inhibitors of the AR or ER, respectively, in in vitro and in vivo experimental models.

14.
Radiol Case Rep ; 17(6): 1831-1835, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35401908

RESUMO

Primary pancreatic lymphoma is a rare disease. It comprises less than 0.5 % of pancreatic neoplasm and 0.1% of malignant lymphoma. It should be differentiated from pancreatic adenocarcinoma because management differs. At computed tomography, 2 types of morphology of primary pancreatic lymphoma have been described- a localized well-circumscribed tumoral form and another diffuse enlargement infiltrating or replacing most of the pancreatic gland. Here, we are presenting computed tomography (CT) imaging features of a case of primary pancreatic lymphoma in a 27 year old female who presented with a complaint of abdominal pain radiating to the back for 3 months and yellowish discoloration of sclera and skin for the last 15 days. In contrast-enhanced computed tomography an exophytic homogenously hypoenhancing mass arising from head and neck region of pancreas was seen. Involvement of common bile duct (CBD) and duodenum was there. The main pancreatic duct was not dilated. Common hepatic artery was encased by mass without arterial luminal narrowing or distortion. Diagnosis of primary pancreatic lymphoma was suggested on basis of imaging findings and further confirmed with fine-needle aspiration cytology.

15.
Molecules ; 27(7)2022 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-35408583

RESUMO

The current study was conducted to examine the in vitro anticancer potential of Cordia dichotoma (bark, leaves, pulp and seed). The plant material was collected from UT of J&K and methodical bioassays were carried out on ten human cancer cell lines (Michigan Cancer Foundation-7 (MCF-7), M.D. Anderson-Metastatic Breast (MDA-MB-231), Neuroblastoma-2a (N2A), SH-SY5Y, U-251, HCT-116, SW-620, A-549, MIA PaCa-2, Panc-1) from five different origins (breast, CNS, colon, lung, pancreas) respectively. Methanolic extracts were produced and fractions were then obtained from the extracts and evaluated for cytotoxicity. Mechanistic assays, HPLC, and GCMS profiling were performed on the highest active fraction. The Sulforhodamine B (SRB) assay determined the in vitro cytotoxicity. The findings revealed that the bark portion had in vitro cytotoxicity against the A-549 human lung cancer cell line. To our knowledge, this is the first study to show that the plant's bark has anticancer properties and induced chromatin condensation, confirmed cell death via ROS generation, and significantly decreased colony formation in A-549 cell line from lung origin in a dose-dependent manner. Furthermore, HPLC and GCMS investigations indicated the presence of a number of bioactive molecules such as gallic acid (144,969.86) uV*sec, caffeic acid (104.26) uV*sec, ferulic acid (472.87) uV*sec, vanillic acid (13,775.39) uV*sec, palmitic acid (18.34%), cis vaccenic acid (28.81%), etc. and one of the compounds was reported for the first time from the bark. As a result of its promising efficacy, it may become an essential cancer chemopreventive or chemotherapeutic medication for patients with lung carcinoma.


Assuntos
Cordia , Neoplasias , Linhagem Celular , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Cordia/química , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Neoplasias/tratamento farmacológico , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/química
16.
J Am Heart Assoc ; 11(6): e023514, 2022 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-35229663

RESUMO

Background Coronary artery bypass can be performed off pump (OPCAB) without cardiopulmonary bypass. However, trends over time for OPCAB versus on-pump (ONCAB) use and long-term outcome has not been reported, nor has their long-term outcome been compared. Methods and Results We queried the national Veterans Affairs database (2005-2019) to identify isolated coronary artery bypass procedures. Procedures were classified as OPCAB on ONCAB using the as-treated basis. Trend analyses were performed to evaluate longitudinal changes in the preference for OPCAB. The median follow-up period was 6.6 (3.5-10) years. An inverse probability weighted Cox model was used to compare all-cause mortality between OPCAB and ONCAB. From 47 685 patients, 6759 (age 64±8 years) received OPCAB (14%). OPCAB usage declined from 16% (2005-2009) to 8% (2015-2019). Patients with triple vessel disease who received OPCAB received a lower mean number of grafts (2.8±0.8 versus 3.2±0.8; P<0.01). The ONCAB 5-, 10-, and 15-year survival rates were 82.9% (82.5-83.3), 60.4% (59.8-61.1), and 37.2% (36.1-38.4); correspondingly, OPCAB rates were 80.7% (79.7-81.7), 57.4% (56-58.7), and 34.1% (31.7-36.6) (P<0.01). OPCAB was associated with increased risk-adjusted all-cause mortality (hazard ratio, 1.15 [1.13-1.18]; P<0.01) and myocardial infarction (incident rate ratio, 1.16 [1.05-1.28]; P<0.01). Conclusions Over 15 years, OPCAB use declined considerably in Veterans Affairs medical centers. In Veterans Affairs hospitals, late all-cause mortality and myocardial infarction rates were higher in the OPCAB cohort.


Assuntos
Ponte de Artéria Coronária sem Circulação Extracorpórea , Infarto do Miocárdio , Veteranos , Idoso , Ponte de Artéria Coronária , Ponte de Artéria Coronária sem Circulação Extracorpórea/métodos , Humanos , Pessoa de Meia-Idade , Resultado do Tratamento
17.
Bioresour Technol ; 351: 127084, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35358671

RESUMO

In this study, a dual chambered microbial fuel cell (MFC) was fabricated for the treatment of tannery wastewater with concurrent production of bio-energy. The tannery effluent acts as an anolyte and a synthetic electrolytic solution as the catholyte. Five electrochemically active bacteria from the biofilm were isolated that showed homology with Klebsiella quasipneumoniae, Klebsiella pneumoniae, Cloacibacterium normanese, Bacillus firmus and Pseudomonas reactans, using 16S rDNA analysis. The physiochemical studies of treated wastewater showcased the 88%, 74% and 94% reduction in COD, BOD and TDS level, respectively. The maximum voltage output and power density obtained using electroactive consortium in MFC was 940 mV and 7371 mW/cm3, respectively. The techno-economic feasibility of the bio-electrochemical system was studied for future bioprospecting. The present study reports a significant power generation with simultaneous effluent treatment up to a maximum of ∼85%, in a sustainable and eco-friendly manner.


Assuntos
Fontes de Energia Bioelétrica , Fontes de Energia Bioelétrica/microbiologia , Biofilmes , Eletricidade , Eletrodos , Águas Residuárias
18.
ACS Appl Mater Interfaces ; 14(10): 12523-12537, 2022 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-35230083

RESUMO

Transition-metal-based layered triple hydroxides (LTHs) are evolving as potential positrode candidates for high-performance supercapacitors; however, their phase stabilization is still critical. Alongside, the availability of limited negatrodes pushes research toward exploring novel alternatives in order to minimize performance limitation issues in the fabricated supercapacitors. Herein, a facile strategy for stabilizing freestanding MnCuCo-LTH-based positrode possessing intermingled nanodisk-needle-like morphology is reported. Alongside, novel high-surface-area negatrodes based on Mn1Fe2S2 exhibiting porous microthorn-like morphology are also optimized. MnCuCo_LTH and Mn1Fe2S2 exhibit remarkably high specific capacities of ∼494 mAh g-1 (∼2540 F g-1) and ∼429 mAh g-1 (∼1546 F g-1), respectively, at 1 A g-1. The fabricated quasi-solid-state supercapacitor equipped with a poly(vinyl alcohol) (PVA)-KOH gel electrolyte displays a high specific capacity of ∼144 mAh g-1 and a specific capacitance of ∼325 F g-1 at 1 A g-1. The ultrahigh energy cum power traits of ∼105 Wh kg-1 (1 A g-1) and ∼8370 W kg-1 (at 10 A g-1) establish an asymmetric supercapacitor as a high-performance energy storage device. This device shows an appreciably high cycling life with a capacitance retention of ∼93% after 10 000 consecutive cycles, at 10 A g-1. This approach provides a neoteric foresight for developing high-performance advanced energy storage devices equipped with cheaper and eco-friendly components.

19.
Am J Transl Res ; 14(2): 1010-1023, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35273703

RESUMO

BACKGROUND: Pleomorphic xanthoastrocytomas (PXAs) are rare, accounting for less than 1% of astrocytomas, and commonly occur in young patients. The majority are WHO grade II. A fraction of tumors that present or recur with malignant change are classified as anaplastic (APXA, grade III). Limited data are available on their molecular characteristics. METHODOLOGY: Genome-wide expression profiling of 14 PXA and 6 APXAs was performed by microarray. Among differentially expressed genes (DEGs), Cyclin-Dependent Kinase 14 (CDK14) and Mitochondrial Fission Process 1 (MTFP1) were validated by qRT PCR. RESULT: Unsupervised hierarchical clustering revealed two distinct molecular clusters (Cluster 1: 10 PXA, 3 APXA and Cluster 2: 4 PXA, 3 APXA) with grade II and III tumors distributed in both highlighting molecular heterogeneity within the same grade. There was an insignificant difference in age, sex, immunohistochemical profile, frequency of BRAF mutation, or CDKN2A deletion among the two clusters. Significantly, worse progression-free survival was observed in cluster 2 (P=0.003). mRNA profiling-based prediction of recurrence was superior to and independent of histological grade, BRAF mutation, or CDKN2A deletion status. A total of 10 upregulated and 418 downregulated genes were identified between the PXA clusters. qRT-PCR validation of CDK14 (upregulated in cluster 2) and MTFP1 (upregulated in cluster 1) showed strong concordance with expression array data. CONCLUSION: This is the first comprehensive study highlighting distinct molecular subgroups of PXA. The differentially expressed genes between two clusters may potentially be used for developing histology independent classification schemes, prognostication and may serve as prospective therapeutic targets for PXA patients.

20.
Indian J Ophthalmol ; 70(3): 783-787, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35225514

RESUMO

PURPOSE: To evaluate and analyze the outcomes of sutureless and glue-free limbal-conjunctival autografting in cases of primary as well as recurrent pterygium. METHODS: This prospective interventional study was carried out between February 2019 and February 2020 at a tertiary care hospital in North India. A.total of 70 patients with pterygium underwent sutureless and glue-free limbal-conjunctival autograft. The patients were divided into two groups: group 1 patients with primary pterygium (n = 45), group 2 patients with recurrent pterygium (n = 25). The patients were followed up till 12 months postoperatively. RESULTS: The mean age of the patients in group 1 and group 2 was 37.04 ± 8.69 years and 32.52 ± 6.49 years, respectively (P = 0.04). Postoperatively, no recurrence was recorded in group 1. Recurrence was noticed in two patients (8%) of group 2. The BCVA changed from 78.73 ± 9.86 letters to 80.15 ± 7.29 letters (P = 0.45) and from 79.6 ± 6.44 letters to 79.8 ± 5.86 letters (P = 0.45) in group 1 and group 2, respectively. Graft edema was found in seven (15.55%) cases of group 1 and four (16%) cases of group 2. Graft retraction was found in two (4.44%) cases of group 1 and three (12%) cases of group 2. CONCLUSION: Sutureless and a glue-free limbal-conjunctival autograft is a safe and effective treatment option for primary as well as recurrent pterygium.


Assuntos
Pterígio , Adulto , Autoenxertos , Túnica Conjuntiva/transplante , Seguimentos , Humanos , Pessoa de Meia-Idade , Projetos Piloto , Complicações Pós-Operatórias , Estudos Prospectivos , Pterígio/diagnóstico , Pterígio/cirurgia , Recidiva , Transplante Autólogo , Resultado do Tratamento
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