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1.
Arch Rehabil Res Clin Transl ; 4(3): 100213, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36123984

RESUMO

Objective: The development and validation of a nomogram for the individualized prediction of hemiplegic shoulder pain (HSP) during the inpatient rehabilitation of patients with stroke. Design: Retrospective cohort study. Setting: The rehabilitation department at a tertiary hospital. Participants: A total of 376 patients (N=376) with stroke admitted to inpatient rehabilitation from January 2018 to April 2021 were included in this study. Interventions: Not applicable. Main Outcome Measures: The outcome measure was shoulder pain on the patients' hemiplegic side occurring at rest or with movement during hospitalization. Results: Among the 376 patients with stroke, 113 (30.05%) developed HSP. Five independent predictors were included in the nomogram: subluxation, Brunnstrom stage, hand edema, spasticity, and sensory disturbance. The nomogram was a good predictor, with a C-index of 0.85 (95% confidence interval, 0.81-0.89) and corrected C-index of 0.84. The Homer-Lemeshow test (χ2=13.854, P=.086) and calibration plot suggested good calibration ability of the nomogram. The optimal cutoff value for the predicted probability of HSP was 0.30 (sensitivity, 0.73; specificity, 0.83). Moreover, the decision curve analysis revealed that the nomogram would add net clinical benefits if the threshold possibility of HSP risk was from 5%-88%. Conclusions: Our nomogram could accurately predict HSP, which may help clinicians accurately quantify the HSP risk in individuals and implement early interventions.

2.
Integr Zool ; 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36049759

RESUMO

Understanding how natural selection shapes unique traits in mammals is a central topic in evolutionary biology. The mammalian order Chiroptera (bats) is attractive for biologists as well as the general public due to their specific traits of extraordinary immunity and inverted resting posture. However, genomic resources for bats that occupy key phylogenetic positions are not sufficient, which hinders comprehensive investigation of the molecular mechanisms underpinning the origin of specific traits in bats. Here, we sequenced the transcriptomes of 5 bats that are phylogenetically divergent and occupy key positions in the phylogenetic tree of bats. In combination with the available genomes of 19 bats and 21 other mammals, we built a database consisting of 10 918 one-to-one ortholog genes and reconstructed phylogenetic relationships of these mammals. We found that genes related to immunity, bone remodeling, and cardiovascular system are targets of natural selection along the ancestral branch of bats. Further analyses revealed that the T cell receptor signaling pathway involved in immune adaptation is specifically enriched in bats. Moreover, molecular adaptations of bone remodeling, cardiovascular system, and balance sensing may help to explain the reverted resting posture in bats. Our study provides valuable transcriptome resources, enabling us to tentatively identify genetic changes associated with bat-specific traits. This work is among the first to advance our understanding of the molecular underpinnings of inverted resting posture in bats, which could provide insight into healthcare applications such as hypertension in humans.

3.
Animals (Basel) ; 12(18)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36139202

RESUMO

Geographic variability in acoustic signals has been documented in many bird species. However, geographic variations in alarm calls have been so far neglected despite their crucial role on reducing risk to group members and relatives. We analyzed the note types and acoustic parameters of Japanese tit (Parus minor) alarm calls to three types of intruders (a nest predator, an adult predator, and a harmless species) from three populations in China. Our results revealed that tits in the same population produce similar note types to different intruders, but the three populations only shared six note types and each population had unique note types. The frequency and duration parameters of three shared common note types were significantly different among populations. The three populations belong to the same species, thus they have shared note types. We suspect that the unique note types occurring in each population may be related to three potential reasons: founder effect, predation pressure, and vocal learning. The differences in acoustic parameters of common notes among populations may be a consequence of adaptations to their environments. We suggest that population differences in the note levels of bird alarm calls do exist.

4.
Tree Physiol ; 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36074523

RESUMO

Drought is one of the major limiting factors in the growth of terrestrial plants. ABA and PYR/PYL/RCARs play a key role in response to drought stress. However, the underlying mechanisms of this control remain largely elusive in trees. In this study, PePYL4, a potential ortholog of the PYR/PYL/RCARs gene, was cloned from Populus euphratica. It was localized in the cytoplasm and nucleus, induced by ABA, osmotic, and dehydration treatments. To study the potential biological functions of PePYL4, transgenic triploid white poplars (Populus tomentosa) overexpressing PePYL4 were generated. PePYL4 overexpression significantly increased ABA sensitivity and reduced stomatal aperture. Compared with wild type plants, transgenic plants had higher water use efficiency (WUE) and lower transpiration. When exposed to drought stress, PePYL4 overexpression plants maintained higher photosynthetic activity and accumulated more biomass. Moreover, overexpression of PePYL4 improved antioxidant enzyme activity and ascorbate content to accelerate reactive oxygen species scavenging. Meanwhile, up-regulation expression of the stress-related genes also contributed to improving the drought tolerance of transgenic plants. In conclusion, our data suggest that PePYL4 is a promising gene target for regulating WUE and drought tolerance in Populus.

5.
Nanoscale Adv ; 4(16): 3362-3369, 2022 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-36131714

RESUMO

Owing to their enhanced catalytic stability and cyclability, two-dimensional (2D) material-supported Pd-based bimetallic alloys have promising applications for catalytic reactions. Furthermore, the alloying strategy can effectively reduce costs and improve catalytic performance. In this paper, we report a one-step reduction method to synthesize a novel heterogeneous catalyst, PdCu@Ti3C2, with good catalytic performance. The composition and structure of the as-prepared catalyst were characterized by inductively coupled plasma-mass spectrometry (ICP-MS), scanning transmission electron microscopy (STEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). The catalyst particles, which were identified as a PdCu bimetallic alloy, exhibited good dispersion on the substrate. The performance of the catalyst in the Suzuki-Miyaura coupling reaction was studied, and the results showed that PdCu@Ti3C2 had excellent catalytic activity, similar to that of homogeneous Pd catalysts such as Pd(PPh3)4. Moreover, the prepared catalyst could be reused at least 10 times in the Suzuki-Miyaura coupling reaction with high yield.

6.
Adv Mater ; : e2204810, 2022 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-35953449

RESUMO

Designing efficient electrocatalysts with high electroconductivity, strong chemisorption, and superior catalytical efficiency to realize rapid kinetics of the lithium polysulfides (LiPSs) conversion process is crucial for practical lithium-sulfur (Li-S) battery applications. Unfortunately, most current electrocatalysts cannot maintain long-term stability due to the possible failure of catalytic sites. Herein, a novel dynamic electrocatalytic strategy with the liquid metal (i.e., gallium-tin, EGaSn) to facilitate LiPSs redox reaction is reported. The combined theoretical simulations and microstructure experiment analysis reveal that Sn atoms dynamically distributed in the liquid Ga matrix act as the main active catalytic center. Meanwhile, Ga provides a uniquely dynamic environment to maintain the long-term integrity of the catalytic system. With the participation of EGaSn, a tailor-made 2 Ah Li-S pouch cell with a specific energy density of 307.7 Wh kg-1 is realized. This work opens up new opportunities for liquid-phase binary alloys as electrocatalysts for high-specific-energy Li-S batteries.

7.
Front Vet Sci ; 9: 937409, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35937300

RESUMO

Foot-and-mouth disease virus (FMDV) is an acute, highly contagious, and economically destructive pathogen of vesicular disease that affects domestic and wild cloven-hoofed animals. The FMDV VP1 protein is an important part of the nucleocapsid and plays a significant role during FMDV infection. However, the signal pathways mediated by VP1 in the life cycle of FMDV and the related mechanisms are not yet fully understood. Here, we performed RNA-seq to compare gene expression profiles between pCAGGS-HA-VP1 transfected PK-15 cells and pCAGGS-HA (empty vector) transfected PK-15 cells. The results showed 5,571 genes with significantly different expression levels, of which 2,981 were up-regulated and 2,590 were down-regulated. GO enrichment analysis showed that 51 GO terms were significantly enriched in cell components including protein complex, membrane and organelle part. KEGG enrichment analysis showed 11 KEGG pathways were significantly enriched which were mainly related to the immune system, infectious viral disease, and signal transduction. Among the up-regulated genes, the chemokines such as CCL5, CXCL8, and CXCL10 in turn promoted FMDV replication. In contrast, GBP1, an interferon-stimulated gene that was suppressed by VP1 and FMDV, could effectively inhibit FMDV replication. Our research provides a comprehensive overview of the response of host cells to VP1 protein and a basis for further research to understand the roles of VP1 in FMDV infection including the genes involved in FMDV replication.

8.
Int J Biol Macromol ; 219: 500-507, 2022 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-35932807

RESUMO

The PA28 family proteasome activators play important roles in regulating proteasome activities. Though the three paralogs (PA28α, PA28ß, and PA28γ) are similar in terms of primary sequence, they show significant differences in expression pattern, cellular localization and most importantly, biological functions. While PA28αß is responsible for promoting peptidase activity of proteasome to facilitate MHC-I antigen processing, but unable to promote protein degradation, PA28γ is well-known to not only promote peptidase activity but also proteolytic activity of proteasome. However, why this paralog has the unique function remains elusive. Previous structural studies have mainly focused on mammalian PA28α, PA28ß and PA28αß heptamers, while structural studies on mammalian PA28γ of atomic resolution are still absent to date. In the present work, we determined the Cryo-EM structure of the human PA28γ heptamer at atomic resolution, revealing interesting unique structural features that may hint our understanding the functional mechanisms of this proteasome activator.

9.
Theranostics ; 12(13): 5727-5743, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35966596

RESUMO

RNA N6 -methyladenosine (m6A) modification and its regulators fine tune gene expression and contribute to tumorigenesis. This study aims to uncover the essential role and the underlying molecular mechanism(s) of the m6A reader YTHDC1 in promoting triple negative breast cancer (TNBC) metastasis. METHODS: In vitro and in vivo models were employed to determine the pathological function of YTHDC1 in TNBC metastasis. To identify bona fide YTHDC1 target RNAs, we conducted RNA-seq, m6A-seq, and RIP-seq, followed by integrative data analysis and validation assays. RESULTS: By analyzing The Cancer Genome Atlas (TCGA) dataset, we found that elevated expression of YTHDC1 is positively correlated with poor prognosis in breast cancer patients. Using a mammary fat pad mouse model of TNBC, YTHDC1 significantly promoted lung metastasis of TNBC cells. Through multiple transcriptome-wide sequencing and integrative data analysis, we revealed dysregulation of metastasis-related pathways following YTHDC1 depletion and identified SMAD3 as a bona fide YTHDC1 target RNA. Depletion of YTHDC1 caused nuclear retention of SMAD3 mRNA, leading to lower SMAD3 protein levels. Loss of YTHDC1 led to impaired TGF-ß-induced gene expression, leading to inhibition of epithelial-mesenchymal transition (EMT) and suppressed TNBC cell migration and invasion. SMAD3 overexpression was able to restore the response to TGF-ß in YTHDC1 depleted TNBC cells. Furthermore, we demonstrated that the oncogenic role of YTHDC1 is mediated through its recognition of m6A as m6A-binding defective mutants of YTHDC1 were unable to rescue the impaired cell migration and invasion of YTHDC1 knockout TNBC cells. CONCLUSIONS: We show that YTHDC1 plays a critical oncogenic role in TNBC metastasis through promoting the nuclear export and expression of SMAD3 to augment the TGF-ß signaling cascade. Overall, our study demonstrates that YTHDC1 is vital for TNBC progression by enhancing TNBC cell survival and TGF-ß-mediated EMT via SMAD3 to enable the formation of distant metastasis and highlights the therapeutic potential of targeting the YTHDC1/m6A/SMAD3 axis for TNBC treatment.


Assuntos
Neoplasias de Mama Triplo Negativas , Animais , Movimento Celular/genética , Transição Epitelial-Mesenquimal/genética , Humanos , Camundongos , Proteínas do Tecido Nervoso/metabolismo , RNA , Fatores de Processamento de RNA/genética , Fatores de Processamento de RNA/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Neoplasias de Mama Triplo Negativas/patologia
10.
Research (Wash D C) ; 2022: 9873564, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35958111

RESUMO

Covalent ligands have attracted increasing attention due to their unique advantages, such as long residence time, high selectivity, and strong binding affinity. They also show promise for targets where previous efforts to identify noncovalent small molecule inhibitors have failed. However, our limited knowledge of covalent binding sites has hindered the discovery of novel ligands. Therefore, developing in silico methods to identify covalent binding sites is highly desirable. Here, we propose DeepCoSI, the first structure-based deep graph learning model to identify ligandable covalent sites in the protein. By integrating the characterization of the binding pocket and the interactions between each cysteine and the surrounding environment, DeepCoSI achieves state-of-the-art predictive performances. The validation on two external test sets which mimic the real application scenarios shows that DeepCoSI has strong ability to distinguish ligandable sites from the others. Finally, we profiled the entire set of protein structures in the RCSB Protein Data Bank (PDB) with DeepCoSI to evaluate the ligandability of each cysteine for covalent ligand design, and made the predicted data publicly available on website.

11.
J Med Chem ; 65(15): 10691-10706, 2022 08 11.
Artigo em Inglês | MEDLINE | ID: mdl-35917397

RESUMO

The past few years have witnessed enormous progress toward applying machine learning approaches to the development of protein-ligand scoring functions. However, the robust performance and wide applicability of scoring functions remain a big challenge for increasing the success rate of docking-based virtual screening. Herein, a novel scoring function named RTMScore was developed by introducing a tailored residue-based graph representation strategy and several graph transformer layers for the learning of protein and ligand representations, followed by a mixture density network to obtain residue-atom distance likelihood potential. Our approach was resolutely validated on the CASF-2016 benchmark, and the results indicate that RTMScore can outperform almost all of the other state-of-the-art methods in terms of both the docking and screening powers. Further evaluation confirms the robustness of our approach that can not only retain its docking power on cross-docked poses but also achieve improved performance as a rescoring tool in larger-scale virtual screening.


Assuntos
Proteínas , Bases de Dados de Proteínas , Ligantes , Simulação de Acoplamento Molecular , Ligação Proteica , Proteínas/metabolismo
12.
BMC Musculoskelet Disord ; 23(1): 809, 2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36002815

RESUMO

BACKGROUND: This study is aimed to investigate retrospectively the radiographic and clinical outcomes in children and young adults with cerebral palsy (CP) undergoing periacetabular osteotomy (PAO) with or without femoral osteotomy (FO) for hip subluxation. METHODS: A consecutive cohort of twenty-one patients (23 hips) with symptomatic CP hip subluxation were treated with PAO with or without FO and reviewed retrospectively. Two patients (2 hips) were excluded due to insufficient follow-up and lost to follow-up, respectively. The Reimers migration percentage, lateral center-edge angle (LCEA), Sharp angle, neck-shaft angle (NSA), femoral anteversion (FNA), Gross Motor Function Classification System (GMFCS) and hip pain were assessed. RESULTS: Twenty-one hips (19 patients) with CP treated with PAO with or without FO were included. Five hips received PAO. Sixteen hips underwent PAO with FO. Mean age at surgery was 19 ± 6 and 15 ± 4 years for PAO and PAO plus FO, respectively. Mean follow-up was 44.0 ± 28.3 months for PAO and 41.5 ± 17.2 months for PAO + FO. All hips were painful before surgery and painless at final visits. The GMFCS improved by one level in 10 of 19 patients. There was significant increase in LCEA (p < 0.001) and decrease in the Reimer's MP (p < 0.001), NSA (p < 0.001) and Tonnis angle(p < 0.001) postoperatively. Resubluxation occurred in 7 hips (30%) due to insufficient correction and loosening of fixation. Nervus cutaneus femoris lateralis was impaired in 4 patients after surgery. There was no avascular necrosis of the femoral head, resubluxation or infection. CONCLUSION: PAO with or without FO can be effective for children and young adults with concomitant hip subluxation and CP.


Assuntos
Paralisia Cerebral , Luxação do Quadril , Luxações Articulares , Acetábulo/cirurgia , Paralisia Cerebral/complicações , Paralisia Cerebral/diagnóstico por imagem , Paralisia Cerebral/cirurgia , Criança , Fêmur/cirurgia , Luxação do Quadril/diagnóstico por imagem , Luxação do Quadril/etiologia , Luxação do Quadril/cirurgia , Articulação do Quadril/cirurgia , Humanos , Luxações Articulares/etiologia , Osteotomia/efeitos adversos , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
13.
Front Cell Infect Microbiol ; 12: 940906, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35873170

RESUMO

Foot-and-mouth disease virus (FMDV) could cause acute infection in host cells, or they could coexist with host cells to generate persistent infection. In persistent infection, the virus could survive for a long time in the host and could be transmitted between different host cells. In the case of FMDV-persistent infection cell line, there is a remarkable significant cellular heterogeneity in the FMDV-persistent infection cell line due to differences of viral load in the individual cells within the cell line. However, the mechanisms of FMDV-persistent infection are not well understood. It is now generally accepted that multiple factors contribute to the coevolution of viruses and cells during the course of persistent infection. The outcome would influence the development of persistent FMDV infection conjointly, reaching a state of equilibrium ultimately. Therefore, in order to elucidate the mechanism of cellular heterogeneity in FMDV-persistent infection cell line, single-cell sequencing was performed on BHK-Op, and pseudotime trajectory plot was draw through cell cluster. Based on the cell clusters, we predicted the development and progression of the FMDV-persistent infection. It could be well explained by the fact that, in BHK-Op cells, there are a fraction of infected cells and a fraction of virus-exposed but uninfected bystander cells. By further comparing the transcripts in cell clusters, we found that these genes were involved in changes in ribosome biogenesis, cell cycle, and intracellular signaling including the interferon signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway. Through comprehensive cross-tabulation analysis of differential expressed genes in various cluster of cells, we identified a high association of Fos, a downstream transcription factor of the MAPK/extracellular signal-regulated kinase (ERK) signaling pathway, with viral replication during the formation of FMDV-persistent infection. Through the further study of Fos, we found that downregulation of Fos facilitates viral clearance during FMDV-persistent infection. Upregulation of c-Raf, which is the upstream of the MAPK/ERK signaling pathway, could promote FMDV replication through downregulation of Fos. Our research is the first to provide insight into the mechanism of the formation FMDV-persistent infection through single-cell sequencing using persistent infection cell line. Pseudotime trajectory analysis was the first time to apply for FMDV-persistent infection cell line. Our work highlights the detailed overview of the evolution of FMDV-persistent infection. We also analyzed the differential expressed genes in the replication or elimination of FMDV within the host. We found that the MAPK/ERK signaling pathway and its downstream transcription factor Fos play an important role in FMDV-persistent infection.


Assuntos
Vírus da Febre Aftosa , Febre Aftosa , Animais , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Febre Aftosa/genética , Vírus da Febre Aftosa/genética , Infecção Persistente , Fatores de Transcrição/metabolismo , Replicação Viral/genética
14.
Sci Total Environ ; 847: 157670, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-35908706

RESUMO

Neonicotinoid insecticides (NIs) are widely used worldwide, accounting for 25 % of the global insecticide market, and are easily transported into surrounding aquatic ecological environments after application. At present, >80 % of surface water is contaminated by NIs globally. Some transformation products (TPs) of NIs can exhibit greater toxicity to aquatic organism than their parent products. However, few studies have evaluated the toxicity and ecological risk of the TPs of NIs. In this study, we aimed to assess the toxicity and ecological risk of seven widely used NIs and their aerobic TPs to aquatic organisms using a prediction method. We found that partial aerobic TPs of NIs have greater toxicity to aquatic organisms than their parent products, and some of them could severely damage aquatic ecosystems. Meanwhile, acetamiprid, thiacloprid, and several other TPs of NIs with a chlorinated ring structure showed strong bioconcentration abilities, which could potentially harm aquatic organisms through the food chain. Moreover, the widespread use of NIs has certain aquatic ecological risks, which should be controlled and limited. This study comprehensively evaluated the ecological risk of seven widely used NIs and their aerobic TPs to aquatic organisms for the first time. Our results could provide an important reference for assessment of the aquatic environmental risk posed by NIs and pollution control.


Assuntos
Inseticidas , Poluentes Químicos da Água , Organismos Aquáticos , Ecossistema , Inseticidas/análise , Neonicotinoides/toxicidade , Água , Poluentes Químicos da Água/análise
15.
Cladistics ; 2022 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-35779275

RESUMO

Marchantiopsida (complex thalloid liverworts) are one of the earliest lineages of embryophytes (land plants), and well-known for their air pores and chambers, pegged rhizoids, and absence of organellular RNA editing sites. Despite their importance to an understanding of early embryophyte evolution, many key nodes within this class remain poorly resolved, owing to the paucity of genetic loci previously available for phylogenetic analyses. Here, we sequenced 54 plastomes, representing 28 genera, nearly all families, and all orders of Marchantiopsida. Based on these plastomes, we present a hypothesis of deep relationships within the class, and make the first investigations of gene contents and synteny. Overall, the Marchantiopsida plastomes were well-conserved, with the exception of the genus Cyathodium that has plastomes with higher GC content, fewer single sequence repeats (SSRs), and more structural variations, implying that this genus might possess RNA editing sites. Abundant repetitive elements and six highly divergent regions were identified as suitable for future infrafamilial taxonomic studies. The phylogenetic topology of Sphaerocarpales, Neohodgsoniales and Blasiales within Marchantiopsida was essentially congruent with previous studies but generally we obtained higher support values. Based on molecular evidence and previous morphological studies, we include Lunulariales in Marchantiales and suggest the retention of narrowed delimitation of monotypic families. The phylogenetic relationships within Marchantiales were better resolved, and 13 monophyletic families were recovered. Our analyses confirmed that the loss of intron 2 of ycf3 is a synapomorphy of Marchantiidae. Finally, we propose a new genus, Asterellopsis (Aytoniaceae), and present an updated classification of Marchantiopsida. The highly supported phylogenetic backbone provided here establishes a framework for future comparative and evolutionary studies of the complex thalloid liverworts.

16.
Ann Clin Lab Sci ; 52(3): 470-474, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35777804

RESUMO

OBJECTIVE: To investigate the impact of different treatment methods on cerebrospinal fluid (CSF) cytokine detection. METHODS: CSF samples were collected from 25 patients. The levels of IL-6, IL-10, IFN-γ, and IL-2 were measured after CSF was stored at room temperature (25°C) or 4°C for 6, 12, and 24 hrs. The CSF was frozen at -80°C, thawed at room temperature for 1 hr every 8 hrs and then frozen. This process was repeated three times in a row, and then cytokine levels in CSF were detected again. RESULTS: The four cytokines were stable when the CSF was kept at room temperature for 6 hrs. After 12 hrs of storage, the levels of the four cytokines decreased, and the changes in IL-6 and IL-10 levels were statistically significant. After 24 hrs of storage, the levels of the four cytokines were further reduced, and the changes were statistically significant. Cytokines were stable when CSF was stored at 4°C, and only IL-10 exhibited statistically significant changes when stored for 24 hrs. IL-6, IL-10 IFN-γ, and IL-2 were stable in CSF samples after three freeze-thaw cycles. CONCLUSION: The stability of CSF cytokines is poor after storage at room temperature and good after storage at 4°C. Therefore, cytokine detection should be carried out after CSF collection as often as possible. If the detection cannot be done quickly enough, the specimens should be stored in cold storage for no more than 24 hrs.


Assuntos
Líquido Cefalorraquidiano , Citocinas , Líquido Cefalorraquidiano/química , Citocinas/química , Humanos , Interleucina-10 , Interleucina-2 , Interleucina-6 , Manejo de Espécimes/métodos
17.
Artigo em Inglês | MEDLINE | ID: mdl-35819496

RESUMO

BACKGROUND: Endoscopic transnasal surgery for tumors in the sellar region has become popular across the globe. However, postoperative nasal outcomes related to endoscopic transnasal surgery have yet to be described in detail. METHOD: Here, we introduce a modified microscopic-endoscopic bilateral transseptal approach for the surgical treatment of tumors in the sellar region. This technique yielded satisfactory postoperative nasal outcomes without any loss of the visual advantages associated with endoscopy. CONCLUSION: The modified microscopic-endoscopic bilateral transseptal approach described herein is a minimally invasive endoscopic transnasal approach and results in satisfactory postoperative outcomes in terms of nasal structure and olfactory function.

18.
Front Psychol ; 13: 891778, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35712217

RESUMO

Background: Psychological dynamics of college students have changed during the COVID-19 outbreak but little research has been done in this area. The purpose of this study is to investigate the dynamic changes in the mental health status of college students since the outbreak of the COVID-19 pandemic 1 year and the influencing factors. Methods: The research period was from February 2020 to August 2021. 384 college students were analyzed three times during this period on the recognition and psychological state of the pandemic. Results: During the period from February 2020 to August 2021, in general, the positive scores rose from 20.79 to 23.46, while the negative scores dropped from 17.41 to 14.00. The regression analysis results on the influencing factors showed the degree of recognition of the pandemic is all significant in the three phases (p < 0.05). Conclusion: With the effective control of the pandemic, the mental state of the students showed a slight improvement in the environment of sporadic cases. Behavior has a partial mediating effect between the source of fear and psychological changes. Correct behavior guidance can effectively reduce the psychological changes caused by college students' fear.

19.
Pharmaceutics ; 14(6)2022 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-35745788

RESUMO

ß-sitosterol (SITO) has been reported with anticancer effects; however, with poor bioavailability. The current study aimed to investigate whether liposomal encapsulated ß-sitosterol (LS) has a better inhibition effect on tumor metastasis than ß-sitosterol in a CT26/luc lung metastasis mouse model and the possible underlying mechanism. LS was liposomal-encapsulated SITO and was delivered to mice by oral gavage. The cell viability was determined by the MTT assay, and invasiveness of the tumor cells and related protein expression were evaluated with the invasion assay and Western blotting. For therapeutic efficacy evaluation, male BALB/c mice were treated with PBS, SITO, and LS once a day for 7 days prior to intravenous injections of CT26/luc cells; treatments were continued twice a week post-cell inoculation throughout the entire experiment. Tumor growth inhibition was monitored by bioluminescent imaging (BLI). IL-12, IL-18, and IFN-γ in the intestinal epithelium were determined by ELISA. The results show that LS treatment had a better invasion inhibition with lower cytotoxicity than SITO when the same dose was utilized. Notably, mice treated with LS significantly exhibited fewer metastases to the lungs and other tissues/organs compared with the Control and SITO groups. Additionally, the IL-12, IL-18, and IFN-γ levels were significantly increased in the LS-treated mice compared with the Control and SITO groups. The underlying mechanism may be through the inhibition of MMP-9 and elicitation of the antitumoral Th1 immune response, such as increasing CD4+ and CD8+ T cells, IL-12, IL-18, and IFN-γ.

20.
Front Plant Sci ; 13: 863389, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35747879

RESUMO

Vacuoles are the most conspicuous organelles in plants for their indispensable functions in cell expansion, solute storage, water balance, etc. Extensive studies on angiosperms have revealed that a set of conserved core molecular machineries orchestrate the formation of vacuoles from multiple pathways. Usually, vacuoles in seed plants are classified into protein storage vacuoles and lytic vacuoles for their distinctive morphology and physiology function. Bryophytes represent early diverged non-vascular land plants, and are of great value for a better understanding of plant science. However, knowledge about vacuole morphology and biogenesis is far less characterized in bryophytes. In this review, first we summarize known knowledge about the morphological and metabolic constitution properties of bryophytes' vacuoles. Then based on known genome information of representative bryophytes, we compared the conserved molecular machinery for vacuole biogenesis among different species including yeast, mammals, Arabidopsis and bryophytes and listed out significant changes in terms of the presence/absence of key machinery genes which participate in vacuole biogenesis. Finally, we propose the possible conserved and diverged mechanism for the biogenesis of vacuoles in bryophytes compared with seed plants.

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