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1.
Biomed Pharmacother ; 143: 112212, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34649345

RESUMO

Coxsackievirus A10 (CVA10) is the main pathogen of hand, foot, and mouth disease in China. However, there are no CVA10-specific drugs and vaccines, and the pathogenesis and effects of this virus in the body are unknown. We investigated the effect of a clinically isolated CVA10 virus strain (CVA10-25) to investigate its effect in suckling mice through different infection routes. We observed the dynamic distribution and proliferation of the virus in mouse tissues by infecting suckling mice with different doses of the virus and mice of different ages with the same dose of the virus. We also analysed the pathological characteristics after infection. A formaldehyde-inactivated experimental vaccine was prepared to immunise 5-week-old BALB/c female mice three times, and newborn suckling mice were tested for the presence of maternally transmitted antibodies. The viral load in each organ after intracerebral administration was higher than that after intraperitoneal administration; the peroral administration route did not cause disease in mice. Mouse paralysis and death after infection were related to age. The skeletal muscles, heart, and lung showed histopathological changes after infection. We established a 2-day-old BALB/c suckling mouse model that could be infected intracranially to study the pathogenesis and pathology of CVA10. Maternally transmitted antibodies protected the mice against the virus. This study provides a reference for CVA10-related pathogenesis and vaccine research.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34492178

RESUMO

Perovskite light-emitting diodes (LEDs) show great potential for next-generation lighting and display technology. Despite intensive studies on single-color devices, there are few reports on perovskite-based white LEDs (Pe-WLEDs). Here, an efficient Pe-WLED based on a blue perovskite and an orange phosphorescent emitter is reported for the first time. It is found that using a simple perovskite/phosphor bilayer emitting structure, there is inefficient energy transfer from the blue perovskite to the orange phosphor, leading to low efficiency and a significant color shift with driving voltage. We address this issue by introducing a quantum-well-like charge-confinement structure for enhancing carrier trapping and thus exciton formation in the phosphorescent emitter. As a result, a high external quantum efficiency of 10.81% is obtained. More interestingly, by tuning the dopant concentration of the phosphorescent emitter using this simple device structure, we can controllably get Pe-WLEDs with very stable white light for display applications or tunable color from warm white to daylight for lighting applications.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34520377

RESUMO

This article addresses the batch-based learning consensus for linear and nonlinear multiagent systems (MASs) with faded neighborhood information. The motivation comes from the observation that agents exchange information via wireless networks, which inevitably introduces random fading effect and channel additive noise to the transmitted signals. It is therefore of great significance to investigate how to ensure the precise consensus tracking to a given reference leader using heavily contaminated information. To this end, a novel distributed learning consensus scheme is proposed, which consists of a classic distributed control structure, a preliminary correction mechanism, and a separated design of learning gain and regulation matrix. The influence of biased and unbiased randomness is discussed in detail according to the convergence rate and consensus performance. The iterationwise asymptotic consensus tracking is strictly established for linear MAS first to demonstrate the inherent principles for the effectiveness of the proposed scheme. Then, the results are extended to nonlinear systems with nonidentical initialization condition and diverse gain design. The obtained results show that the distributed learning consensus scheme can achieve high-precision tracking performance for an MAS under unreliable communications. The theoretical results are verified by two illustrative simulations.

4.
Br J Nutr ; : 1-12, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34526168

RESUMO

Chronic inflammation exerts pleiotropic effects in the aetiology and progression of chronic obstructive pulmonary disease (COPD). Glucosamine is widely used in many countries and may have anti-inflammatory properties. We aimed to prospectively evaluate the association of regular glucosamine use with incident COPD risk and explore whether such association could be modified by smoking in the UK Biobank cohort, which recruited more than half a million participants aged 40-69 years from across the UK between 2006 and 2010. Cox proportional hazards models with adjustment for potential confounding factors were used to calculate hazard ratios (HR) as well as 95 % CI for the risk of incident COPD. During a median follow-up of 8·96 years (interquartile range 8·29-9·53 years), 9016 new-onset events of COPD were documented. We found that the regular use of glucosamine was associated with a significantly lower risk of incident COPD with multivariable adjusted HR of 0·80 (95 % CI, 0·75, 0·85; P < 0·001). When subgroup analyses were performed by smoking status, the adjusted HR for the association of regular glucosamine use with incident COPD were 0·84 (0·73, 0·96), 0·84 (0·77, 0·92) and 0·71 (0·62, 0·80) among never smokers, former smokers and current smokers, respectively. No significant interaction was observed between glucosamine use and smoking status (Pfor interaction = 0·078). Incident COPD could be reduced by 14 % to 84 % through a combination of regular glucosamine use and smoking cessation.

5.
Eur J Endocrinol ; 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34524977

RESUMO

Objective To examine the association of incident type 2 diabetes (T2D) risk with sleep factors, genetic risk, and their combination effects. Design Large prospective population-based cohort study. Methods This population-based prospective cohort study included 360 403 (mean [SD] age: 56.6 [8.0] years) participants without T2D at baseline from the UK Biobank. Genetic risk was categorized as high (highest quintile), intermediate (quintiles 2 to 4), and low (lowest quintile) based on a polygenic risk score for T2D. Sleep scores, including long or short sleep duration, insomnia, snoring, late chronotype, and excessive daytime sleepiness, were categorized as an unfavourable, intermediate, or favourable sleep and circadian pattern. Results During a median follow-up of 9.0 years, 13 120 incident T2D cases were recorded. Among the participants with an unfavourable sleep and circadian pattern, 6.96% (95% CI, 6.68%-7.24%) developed T2D versus 2.37% (95% CI, 2.28%-2.46%) of participants with a favourable sleep and circadian pattern (adjusted HR: 1.53, 95% CI: 1.45-1.62). Of participants with a high genetic risk, 5.53% (95% CI, 5.36%-5.69%) developed T2D versus 2.01% (95% CI, 1.91%-2.11%) of participants with a low genetic risk (adjusted HR: 2.89, 95% CI: 2.72-3.07). The association with sleep and circadian patterns was independent of genetic risk strata. Participants in the lowest quintile with an unfavourable sleep and circadian pattern were 3.97-fold more likely to develop T2D than those in the lowest quintile with a favourable sleep and circadian pattern. Conclusions Sleep and circadian patterns and genetic risk were independently associated with incident T2D. These results indicate the benefits of adhering to a healthy sleep and circadian pattern in entire populations, independent of genetic risk.

6.
Phytother Res ; 2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34355433

RESUMO

Currently available therapies for hepatocellular carcinoma (HCC), with a high morbidity and high mortality, are only marginally effective and with sharp adverse side effects, which makes it compulsory to explore novel and more effective anticancer molecules. Chinese medicinal herbs exhibited prominent anticancer effects and were applied to supplement clinical cancer treatment. Here, we reported a compound, trilobolide-6-O-isobutyrate (TBB), isolated from the flowers of Wedelia trilobata with a markedly cytotoxic effect on HCC cells. We found that TBB time- and dose-dependently inhibited HCC cells' growth and colony formation in vitro. Moreover, TBB induced cell cycle arrest at the G2/M phase, mitochondrial caspase-dependent apoptosis, and suppressed migration and invasion, as well as the glycolysis of HCC cells. Mechanistically, our data indicated that TBB inhibited the STAT3 pathway activation by directly interacting with the TYR 640/657 sites of the STAT3 protein and decreasing the level of p-STAT3. TBB also regulated the expression of PCNA, Ki67, Cyclin B1, Cyclin E, Bax, Bcl2, MMP2/9, and PGK1 through the inhibition of the IL-6/STAT3 signaling pathway. Lastly, we confirmed that TBB effectively eliminated tumor growth without causing overt toxicity to healthy tissues in the xenograft tumor model. The exploration of anticancer activity and the underlying mechanism of TBB suggested its usage as a promising chemotherapeutic agent for HCC.

7.
Artigo em Inglês | MEDLINE | ID: mdl-34462721

RESUMO

Because of the relatively limited understanding of COVID-19 pathogenesis, immunological analysis for vaccine development is needed. Mice and macaques were immunized with an inactivated SARS-CoV-2 vaccine prepared by two inactivators. Various immunological indexes were tested, and viral challenges were performed on day 7 or 150 after booster immunization in monkeys. This inactivated SARS-CoV-2 vaccine was produced by sequential inactivation with formaldehyde followed by propiolactone. The various antibody responses and specific T cell responses to different viral antigens elicited in immunized animals were maintained for longer than 150 days. This comprehensive immune response could effectively protect vaccinated macaques by inhibiting viral replication in macaques and substantially alleviating immunopathological damage, and no clinical manifestation of immunopathogenicity was observed in immunized individuals during viral challenge. This candidate inactivated vaccine was identified as being effective against SARS-CoV-2 challenge in rhesus macaques.

8.
Ann Am Thorac Soc ; 2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34242153

RESUMO

RATIONALE: Both genetic variants and chronic obstructive pulmonary disease (COPD) contribute to the risk of incident severe coronavirus disease 2019 (COVID-19). Whether genetic risk of incident severe COVID-19 is the same regardless of pre-existing COPD is unknown. OBJECTIVES: In this study, we aimed to investigate the potential interaction between genetic risk and COPD in relation to severe COVID-19. METHODS: We constructed a polygenic risk score (PRS) for severe COVID-19 by using 112 single-nucleotide polymorphisms in 430,582 participants from the UK Biobank study. We examined the associations of genetic risk and COPD with severe COVID-19 by using logistic regression models. RESULTS: Of 430,582 participants, 712 participants developed severe COVID-19 as of February 22, 2021, of whom 19.8% had pre-existing COPD. Compared with participants at low genetic risk, those at intermediate genetic risk (OR, 1.34; 95% CI, 1.09-1.66) and high genetic risk (OR, 1.50; 95% CI, 1.18-1.92) had higher risk of severe COVID-19 (P for trend = 0.001), and the association was independent of COPD (P for interaction = 0.76). COPD was associated with a higher risk of incident severe COVID-19 (OR, 1.37; 95% CI, 1.12-1.67; P = 0.002). Participants at high genetic risk and with COPD had a higher risk of severe COVID-19 (OR, 2.05; 95% CI, 1.35-3.04; P < 0.001) than those at low genetic risk and without COPD. CONCLUSIONS: The PRS which combines multiple risk alleles can be effectively used in screening for high-risk populations of severe COVID-19. High genetic risk correlates with a higher risk of severe COVID-19, regardless of pre-existing COPD.

9.
Eur J Pharmacol ; 908: 174370, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34324855

RESUMO

Colorectal cancer (CRC) is the most fatal gastrointestinal tumor and it is urge to explore powerful drugs for the treatment. Diosgenin (DSG) as a new steroidal had been reported exerts anti-tumor activity in multiple cancers, including CRC. However, the potential mechanism of DSG suppresses CRC remains further to be revealed. Here, we reported that DSG inhibited proliferation of CRC cells in dose- and time-dependent manner, induced apoptosis by modulating p53 and Bcl-2 family proteins expression to mediate mitochondrial apoptosis pathway, suppressed migration and invasion by reducing MMP-9 (matrix metalloproteinase) and decreased aerobic glycolysis by mediating glucose transporter (GLUT) like GLUT3 and GLUT4, and pyruvate carboxylase PC downregulation. Intriguingly, mechanistic study suggests those phenotypes involved DSG inhibited cAMP/PKA/CREB pathway in CRC cells, and result to inhibit the phosphorylation of CREB to regulate the transcription of genes above-mentioned. Finally, nude mice xenograft tumor model further indicated that DSG could be a great agent to suppress the growth of CRC cells in vivo and have no obvious side effects. Taken together, we revealed a unique mechanism that DSG suppresses CRC cells through cAMP/PKA/CREB pathway and DSG is a promising candidate drug for CRC treatment.

10.
ACS Appl Mater Interfaces ; 13(26): 30274-30283, 2021 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-34170100

RESUMO

In this work, an iron self-boosting polymer nanoenzyme was prepared by using pyrrole-3-carboxylic acid as a monomer and iron as an oxidizing agent via a simple and one-step method [hereafter referred to as FePPy nanoparticles (NPs)]. In fact, researchers previously paid negligible attention on the iron element during the polymerization reaction of polypyrrole, thus the intrinsically catalytic functions and enzymatic activities of the high iron content (wt %: 21.11%) are ignored and not fully explored. As expected, results demonstrate that the as-synthesized FePPy NPs can decompose H2O2 to generate hydroxyl radicals (•OH) which exhibit enzyme characteristics, further inducing a nonapoptotic ferroptosis pathway. Moreover, the nanoenzyme shows impressive photothermal properties which can accelerate the Fenton reactions to enhance ferroptosis. The combined photothermal and ferroptosis therapy of FePPy NPs was found to have high efficacy. With the properties of easy synthesis, high efficacy, and good biocompatibility, the FePPy NPs are considered as potential agents for cancer treatments.


Assuntos
Antineoplásicos/uso terapêutico , Ferroptose/efeitos dos fármacos , Nanoestruturas/uso terapêutico , Neoplasias/tratamento farmacológico , Fármacos Fotossensibilizantes/uso terapêutico , Animais , Antineoplásicos/química , Antineoplásicos/efeitos da radiação , Ácidos Carboxílicos/química , Ácidos Carboxílicos/efeitos da radiação , Ácidos Carboxílicos/uso terapêutico , Catálise , Feminino , Células HeLa , Humanos , Peróxido de Hidrogênio/química , Radical Hidroxila/metabolismo , Ferro/química , Ferro/efeitos da radiação , Luz , Camundongos Endogâmicos BALB C , Camundongos Nus , Nanoestruturas/química , Nanoestruturas/efeitos da radiação , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/efeitos da radiação , Terapia Fototérmica , Polímeros/química , Polímeros/efeitos da radiação , Polímeros/uso terapêutico , Pirróis/química , Pirróis/efeitos da radiação , Pirróis/uso terapêutico , Temperatura
11.
Adv Mater ; 33(29): e2008487, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34085738

RESUMO

α-Formamidinium lead triiodide (α-FAPbI3 ) represents the state-of-the-art for perovskite solar cells (PSCs) but experiences intrinsic thermally induced tensile strain due to a higher phase-converting temperature, which is a critical instability factor. An in situ crosslinking-enabled strain-regulating crystallization (CSRC) method with trimethylolpropane triacrylate (TMTA) is introduced to precisely regulate the top section of perovskite film where the largest lattice distortion occurs. In CSRC, crosslinking provides in situ perovskite thermal-expansion confinement and strain regulation during the annealing crystallization process, which is proven to be much more effective than the conventional strain-compensation (post-treatment) method. Moreover, CSRC with TMTA successfully achieves multifunctionality simultaneously: the regulation of tensile strain, perovskite defects passivation with an enhanced open-circuit voltage (VOC  = 50 mV), and enlarged perovskite grain size. The CSRC approach gives significantly enhanced power conversion efficiency (PCE) of 22.39% in α-FAPbI3 -based PSC versus 20.29% in the control case. More importantly, the control PSCs' instability factor-residual tensile strain-is regulated into compression strain in the CSRC perovskite film through TMTA crosslinking, resulting in not only the best PCE but also outstanding device stability in both long-term storage (over 4000 h with 95% of initial PCE) and light soaking (1248 h with 80% of initial PCE) conditions.

12.
Mol Genet Genomic Med ; 9(8): e1697, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34145795

RESUMO

BACKGROUND: Isocitrate dehydrogenase (IDH) is an enzyme family involved in cell aerobic metabolism of tricarboxylic acid cycle. However, the landscape of IDH mutations in pan-cancer has not been fully characterized. METHODS: Tissue or blood samples were subjected to next-generation sequencing (NGS) for detection the IDH mutation. RESULTS: A total of 28.868 patients from more than 20 solid tumor species were analyzed. A total of 374 cases (1.30%) with IDH mutations were identified. Among all the IDH mutations cases, 80 (21.4%) were biliary tract cancer (BTC), 80 (21.4%) were lung cancer, 57 (15.2%) were liver cancer, and 42 (11.2%) were colorectal cancer. The most common IDH variant were IDH1 and IDH2 which were discovered in 0.81% cases and 0.47% cases, respectively. However, there were significant differences in IDH1 and IDH2 mutation frequency among different tumor species (p = 0.0003). Of the patients with IDH1 mutations, about 53.0% of these mutations occur in codons 132. Codons 172 (25.4%) was high-frequency mutation subtypes in IDH2 mutation. TP53, PBRM1, and BAP1 were the most significantly mutated genes in BTC which were different from others cancer. Moreover, TMB were significantly higher in lung cancer, colorectal cancer, and gastric cancer than BTC (p = 0.0164, p < 0.0001, p = 0.0067, respectively) and BTC patients with IDH mutation had lower TMB compared with wild-type IDH. CONCLUSION: Somatic IDH mutation was found in multiple solid tumors and IDH would be a driver gene in BTC.

13.
Small ; : e2008182, 2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34106511

RESUMO

Aqueous Zn ion batteries (AZIBs), featuring low cost, long-term cycling stability, and superior safety are promising for applications in advanced energy storage devices. However, they still suffer from unsatisfactory energy density and operating voltage, which are closely related to cathode materials used. Herein, the use of monoclinic MnV2 O6 (MVO) is reported, which can be activated for high-capacity Zn ions storage by electrochemically oxidizing part of the Mn2+ to Mn3+ or Mn4+ while the remaining Mn2+ ions act as binders/pillars to hold the layer structure of MVO and maintain its integrity during charging/discharging process. Moreover, after introducing carbon nanotubes (CNT), the MVO:CNT composite not only provides robust 3D Zn-ion diffusion channels but also shows enhanced structural integrity. As a result, a MVO:CNT cathode delivers a high midpoint voltage (1.38 V after 3000 cycles at 2 A g-1 ) and a high energy density of 597.9 W h kg-1 . Moreover, DFT analyses clearly illustrate stepwise Zn ion insertion into the MnV2 O6 lattice, and ex-situ analyses results further verify the highly structural reversibility of the MnV2 O6 cathode upon extended cycling, demonstrating the good potential of MnV2 O6 for the establishment of viable aqueous Zn ion battery systems.

14.
Insect Sci ; 2021 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-34080301

RESUMO

The eicosanoid signaling pathway mediates insect immune reactions to a wide range of stimuli. This pathway begins with the biosynthesis of arachidonic acid (AA) from the hydrolysis of phospholipids catalyzed by phospholipase A2 (PLA2 ). We report here that the PLA2 inhibitor, dexamethasone (DEX), impaired the innate immune response including nodulation, encapsulation, and melanization in Ostrinia furnacalis larvae, while AA partially reversed these effects of DEX. We cloned a full-length complementary DNA encoding a PLA2 , designated as OfsPLA2 , from O. furnacalis. The open reading frame of OfsPLA2 encodes a 195-amino acid residue protein with a 22-residue signal peptide. Sequence alignment analyses indicated that O. furnacalis PLA2 might be a Group III secretory PLA2 . The highest transcript levels of OfsPLA2 were detected in the fat body, and its transcript levels increased dramatically after infection with Escherichia coli, Micrococcus luteus, or Beauveria bassiana. Recombinant OfsPLA2 significantly induced prophenoloxidase (PPO) activation in larval hemolymph in the presence of Ca2+ and encapsulation of agarose beads. Injection of recombinant OfsPLA2 into larvae resulted in increased transcript levels of attacin, defencin, and moricin-3 genes. Our results demonstrate the involvement of the eicosanoid signaling pathway in the innate immune response of O. furnacalis larvae and provide new information about the roles of O. furnacalis secretory PLA2 in activating PPO and antimicrobial peptide production.

15.
Artigo em Inglês | MEDLINE | ID: mdl-34129506

RESUMO

In this article, we consider quantized learning control for linear networked systems with additive channel noise. Our objective is to achieve high tracking performance while reducing the communication burden on the communication network. To address this problem, we propose an integrated framework consisting of two modules: a probabilistic quantizer and a learning scheme. The employed probabilistic quantizer is developed by employing a Bernoulli distribution driven by the quantization errors. Three learning control schemes are studied, namely, a constant gain, a decreasing gain sequence satisfying certain conditions, and an optimal gain sequence that is recursively generated based on a performance index. We show that the control with a constant gain can only ensure the input error sequence to converge to a bounded sphere in a mean-square sense, where the radius of this sphere is proportional to the constant gain. On the contrary, we show that the control that employs any of the two proposed gain sequences drives the input error to zero in the mean-square sense. In addition, we show that the convergence rate associated with the constant gain is exponential, whereas the rate associated with the proposed gain sequences is not faster than a specific exponential trend. Illustrative simulations are provided to demonstrate the convergence rate properties and steady-state tracking performance associated with each gain, and their robustness against modeling uncertainties.

16.
Aging (Albany NY) ; 13(16): 20094-20115, 2021 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-34032605

RESUMO

BACKGROUND: Hepatocellular carcinoma (HCC) is the main type of primary liver cancer and shows a heavy burden worldwide. Its recurrence and mortality rate are still uncontrolled by the usage of present treatments. More attention has been focused on exploring specific genes that play important roles in HCC procession, and the function of DEP domain containing 1B (DEPDC1B) in HCC has not been researched. METHODS: Immunohistochemical staining was used to detect the expression level of DEPDC1B in tumor tissues and adjacent normal tissues. After DEPDC1B and CDK1 knockdown in cell lines HEP3B2.1-7 and SK-HEP-1, MTT assay and colony formation assay was used to detect cell growth, flow cytometry assay was used to investigate cell apoptosis and cell cycle, wound-healing assay and Transwell assay were used to examine the tumor cell migration. Moreover, a xenograft model was constructed to research functions of DEPDC1B in tumor growth in vivo. RESULTS: The results show that DEPDC1B knockdown inhibit the progression of HCC, through inhibiting cell proliferation, migration, colony formation, leading to G2 phase arrest, and promoting cell apoptosis in vitro, and CDK1 was selected for further mechanic research according to the results of Human GeneChip prime view. The results of recovery experiment displayed that the functions of DEPDC1B on HCC progression were mediated by CDK1. DEPDC1B knockdown can also inhibit tumor growth in vivo. CONCLUSIONS: The study confirmed that DEPDC1B knockdown restrains the tumor growth in vitro and vivo, and it can interact with CDK1 and rescued by CDK1. The study suggested that DEPDC1B was as a potential therapeutic target involved in HCC growth and progression.

17.
Clin Nutr ; 40(5): 2620-2629, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33933728

RESUMO

BACKGROUND & AIMS: The association between dietary diversity (DD) changes and mortality remains unclear. We aimed to investigate the association between DD changes and all-cause mortality among older people. METHODS: A total of 17,959 participants with a mean age of 84.8 years old were enrolled at baseline. Food groups were collected at baseline and follow-up using simplified food frequency questionnaire (FFQ), and then overall, plant-based and animal-based dietary diversity score (DDS) were calculated. DDS changes were calculated using DDS at baseline and the first follow-up. The association between three DDS changes (overall, plant-based and animal-based DDS) and subsequent all-cause mortality were evaluated. Nonparametrically restricted cubic splines and a multivariable-adjusted Cox proportional hazards model were used to estimate HRs and 95% CIs. RESULTS: We documented 12,974 deaths over a 129,590 person-years of follow up. Compared with high-to-high DDS pattern, participants with lower overall DDS patterns had increased mortality risk with HRs (95%CI) of 1.39 (1.29-1.49), 1.53 (1.37-1.70), 1.38 (1.18-1.60) and 1.55 (1.31-1.83) for medium-to-medium, low-to-low, low-to-high and high-to-low patterns, respectively. And compared with high-to-high DDS pattern, the estimates were 1.34 (1.23-1.46), 1.49 (1.35-1.65), 1.43 (1.23-1.67) and 1.62 (1.40-1.88) for plant-based DDS, and 1.23 (1.15-1.31), 1.29 (1.20-1.40), 1.24 (1.12-1.37) and 1.28 (1.15-1.44) for animal-based DDS for medium-to-medium, low-to-low, low-to-high and high-to-low patterns, respectively. There was a U-shaped association between DDS change scores and mortality, and compared with participants with whose DDS remained stable, those with extreme declines and extreme improvements had higher risks of mortality with HRs (95% CI) of 1.15 (1.09-1.22) and 1.11 (1.04-1.17). CONCLUSIONS: Maintaining a lower DDS, extreme declines and extreme improvements in DDS were all associated with an increased risk of all-cause mortality.


Assuntos
Dieta/classificação , Idoso , Idoso de 80 Anos ou mais , Causas de Morte , Estudos de Coortes , Inquéritos sobre Dietas , Comportamento Alimentar , Feminino , Humanos , Masculino , Estado Nutricional , Estudos Prospectivos
18.
J Int Med Res ; 49(5): 3000605211004414, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33983053

RESUMO

OBJECTIVE: To examine the role of the long noncoding RNA LINC01296 in colorectal carcinoma (CRC) and to explore the underlying mechanism. METHODS: We detected LINC01296 expression levels in a cohort of 51 paired CRC and normal tissues. We also assessed the effects of LINC01296 on cell proliferation and apoptosis in CRC cells in vitro, and measured its effect on tumor growth in an in vivo mouse model. We identified the potential downstream targets of LINC01296 and assessed its regulatory effects. RESULTS: Expression levels of LINC01296 were elevated in 37/51 CRC tissues compared with the corresponding normal tissues and were significantly associated with tumor stage, lymph node metastasis, and distant metastasis. Knockdown of LINC01296 using antisense oligonucleotides inhibited cell proliferation and promoted apoptosis of colon cancer cells in vitro and inhibited tumor growth in vivo. Knockdown of LINC01296 also significantly increased the gene expression of p15 in colon cancer cells. LINC01296-specific suppression of p15 was validated by the interaction between enhancer of zeste homolog 2 and LINC01296. CONCLUSION: Overexpression of LINC01296 suppressed the expression of p15 leading to CRC carcinogenesis. These findings may provide the basis for novel future CRC-targeted therapies.


Assuntos
Neoplasias do Colo , Neoplasias Colorretais , RNA Longo não Codificante , Animais , Apoptose/genética , Carcinogênese/genética , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células/genética , Neoplasias Colorretais/genética , Regulação Neoplásica da Expressão Gênica , Camundongos , RNA Longo não Codificante/genética
19.
Medicine (Baltimore) ; 100(15): e25237, 2021 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-33847621

RESUMO

OBJECTIVE: This study aimed to investigate the clinicopathological features and the survival outcomes of neuroendocrine prostate cancer (NEPC). METHODS: Within the Surveillance, Epidemiology, and End Results (SEER) database of the National Cancer Institute, we identified a total of 510 patients with NEPC between 2006 and 2015. Age-adjusted incidence rates were evaluated in the study by the SEER∗Stat Software version 8.3.6. Kaplan-Meier analysed assessed overall survival (OS) after stratification according to marital status, age, histologic subtype, metastatic status, and treatment. The significant differences were assessed in a log-rank test. Univariate and multivariate cox hazard regression analysis were performed to determine independent predictors of OS. RESULTS: From a total of 560,124 patients with prostate cancer diagnosed between 2006 and 2015, we identified 510 cases of de novo NEPC. Regarding histology, among all the NEPC, 329 (64.5%) patients were diagnosed as small cell carcinoma, 181 (39.8%) were nonsmall cell carcinoma. The overall age-adjusted incidence of NEPC statistically significantly increased from 0.321/1,000,000 person-years in 2006 to 0.587/1,000,000 person-years in 2015. The median OS in our study cohort was 9 months (95% CI, 8-10 months). Multivariate cox regression analysis showed that age, histologic subtype, and stage were independent prognostic factors for NEPC patients. The majority of NEPC (78.2%) were metastatic at diagnosis. In terms of treatment, for metastatic tumor patients, chemotherapy was the most effective therapy. Chemotherapy increased the OS of patients with regional (distant) metastases from 8 months (5 months) to 13.5 months (9 months). CONCLUSION: NEPC is extremely rare but the incidence of NEPC has been increasing in the past years. The prognosis of NEPC is poor because most cases are diagnosed at metastatic stage. The patients with metastases are typically treated with chemotherapy and chemotherapy shows survival benefits in both regional and distant metastatic tumor patients.


Assuntos
Carcinoma Neuroendócrino/mortalidade , Carcinoma Neuroendócrino/patologia , Neoplasias da Próstata/mortalidade , Neoplasias da Próstata/patologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Carcinoma Neuroendócrino/terapia , Humanos , Incidência , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica , Estadiamento de Neoplasias , Prognóstico , Neoplasias da Próstata/terapia , Programa de SEER , Fatores Socioeconômicos
20.
BMC Public Health ; 21(1): 796, 2021 04 26.
Artigo em Inglês | MEDLINE | ID: mdl-33902517

RESUMO

BACKGROUND: We investigated college students' attitude and compliance towards a prevention strategy involving use of non-pharmaceutical interventions (NPIs) against coronavirus disease 2019 (COVID-19). METHODS: We conducted a cross-sectional online survey in four universities in Guangdong Province (China) based on purposive sampling. A self-administered questionnaire was given to College students (CSs) to measure the supportive attitude towards an outbreak control strategy and adoption of NPIs in respondents. RESULTS: A total of 44,446 CSs participated between 31 January and 10 February 2020; 92.7% of respondents supported the outbreak control strategy. The proportion of respondents who avoided public places, wore a facemask, avoid gatherings, and washed hands more frequently than usual was 94.8, 92.8, 91.2 and 86.9%. respectively. A total of 76.5% respondents adopted all four measures. A supportive attitude was associated with NPI adoption. Students who were female, postgraduate, anxious, and not depressed tended to have a higher supportive attitude and higher chance of NPI adoption. CONCLUSIONS: Higher supportiveness towards the disease control strategy for the Chinese public may lead to higher adoption rate of NPIs. Psychosocial factors were related to a supportive attitude and adoption of the NPI. We believe that our findings could aid policymakers to create NPIs to prevent and control emerging infectious diseases such as COVID-19.


Assuntos
COVID-19 , China , Estudos Transversais , Feminino , Humanos , Masculino , SARS-CoV-2 , Estudantes , Inquéritos e Questionários
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