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1.
Molecules ; 26(19)2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34641341

RESUMO

INTRODUCTION: A novel analytical method using fast gas chromatography combined with surface acoustic wave sensor (GC-SAW) was developed for rapid determination of the pharmacological volatiles of turmeric (Curcuma longa L.). METHODS: The volatile compounds in 20 turmeric samples, collected from different parts and different origins, were assessed by the fast GC-SAW. In addition, gas chromatography-mass spectrometry (GC-MS) was employed to confirm the chemical composition of the main volatiles. The digital fingerprint of turmeric was established and analysed by principal component analysis and cluster analysis. RESULTS: Curcumene (9.1%), ß-sesquiphellandrene (5.1%) and ar-turmerone (69.63%) were confirmed as the main pharmacological volatiles of turmeric. The content of ar-turmerone in lateral rhizome turmeric was significantly higher than that of top rhizome and ungrouped turmeric. The contents of curcumene and ß-sesquiphellandrene in top rhizome turmeric were higher than those in lateral and ungrouped turmeric. The 20 turmeric samples were divided into four categories, which reflected the quality characteristics of the turmeric from different parts and origins. CONCLUSION: The GC-SAW method can rapidly and accurately detect pharmacologically volatiles of turmeric, and it can be used in the quality control of turmeric.

2.
J Cardiothorac Surg ; 16(1): 273, 2021 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-34565436

RESUMO

BACKGROUND: Synchronous multiple primary lung cancers are becoming more common with increasing use of computed tomography for screening. Intraoperative localization and resection of ill-defined pulmonary ground-glass opacities during thoracoscopic resection is challenging. This study aimed to determine the clinical feasibility of non-invasive visual localization of these nodules by three-dimensional computed tomography lung reconstruction before sublobar resection. METHODS: Forty-four patients with synchronous multiple primary lung cancers underwent thoracoscopic pulmonary resection at our institution between June 2017 and August 2019. Preadmission computed tomography images were downloaded and reconstructed into a three-dimensional model. Small nodules (< 15 mm) were localized non-invasively by three-dimensional computed tomography lung reconstruction before surgery. Patient demographics, nodule characteristics, procedural details, pathological data, and outcomes were obtained from the medical records. RESULTS: One hundred and twenty-one pulmonary nodules from the 44 patients were scheduled for video-assisted thoracic surgery; 54 (44.6%) were pure ground-glass opacities and 57 (47.1%) were mixed ground-glass opacities. One hundred and seventeen nodules were localized preoperatively. The mean nodule diameter was 7.67 ± 3.87 mm. The mean distance from the nodule to the pleura was 14.84 ± 14.43 mm. All nodules were removed successfully by wedge resection (27 patients), lobectomy (26 patients), or segmentectomy (25 patients). Most lesions (85.1%) were malignant. Paraffin pathology revealed 12 cases of atypical adenomatous hyperplasia (9.92%), 13 of adenocarcinoma in situ (10.74%), 16 of minimally invasive adenocarcinoma (13.22%), and 73 of invasive adenocarcinoma (60.33%). CONCLUSIONS: Three-dimensional computed tomography lung reconstruction is a feasible and alternative method of visual localization for small lung nodules before sublobar resection in some suitable patients.

3.
Transfusion ; 61(9): E57-E58, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34314514
4.
Cell Death Dis ; 12(8): 717, 2021 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-34285190

RESUMO

Renal cell carcinoma (RCC) cells have increased lipogenesis and cholesterol synthesis. Sterol regulatory element-binding protein-1 (SREBP1) is cleaved by site 1 protease (S1P) to release the transcriptionally active amino-terminal domain. PF-429242 is a potent and competitive S1P inhibitor. We here tested its activity in RCC cells. In established and primary human RCC cells, PF-429242 potently inhibited cell proliferation, migration, and invasion. The S1P inhibitor provoked apoptosis activation in RCC cells. Furthermore, shRNA-mediated S1P silencing or CRISPR/Cas9-induced S1P knockout led to RCC cell growth inhibition and apoptosis activation. Conversely, ectopic overexpression of SREBP1 or S1P augmented RCC cell proliferation and migration. Daily i.v. injection of a single dose of PF-429242 robustly inhibited RCC xenograft growth in severe combined immunodeficiency mice. Additionally, intratumoral injection of S1P shRNA lentivirus inhibited RCC xenograft growth in mice. SREBP1, S1P, and its target gene low density lipoprotein receptor (LDLR) were significantly elevated in human RCC tissues. These results suggest that targeting S1P by PF-429242 inhibited RCC cell growth in vitro and in vivo.

5.
Methods ; 2021 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-34090972

RESUMO

Intensive and lasting stress may induce severe damage to a human's physical and mental health. Successful stress management depends on the effective monitoring of people's everyday activities, in particular, their sedentary behaviors. Here, we propose an unobtrusive office sedentary behavior monitoring system that combines Bluetooth signals and ballistocardiogram (BCG) signals to classify an individual's sitting modes into four categories: off-seat, sedate, working, and in-motion. The proposed monitoring system simultaneously reads received signal strength indicators (RSSI) from several fixed Bluetooth Low Energy (BLE) beacons and BCG data from the piezoelectric sensor placed underneath the chair cushion, with distinct sampling frequencies. The raw signals are first denoised with local subspace projection. Then we extract the local spectral features from the reconstructed signal and the signal differences for a two-stage stacking learning algorithm. The temporally classified results establish a desk-based worker's sedentary profile and make possible the timely intervention of physical inactivity. We tested the prototype system for 15 subjects, and the preliminary results achieved 95% accuracy, demonstrating its potential in a real-world application.

6.
Urolithiasis ; 2021 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-34050772

RESUMO

Calcium oxalate (CaOx) is a major contributor to urolithiasis, one of the most common urological diseases. Our previous study has shown that Klotho rs3752472 polymorphism correlates with an increased risk of CaOx-related urolithiasis in human cohorts. This study aims to identify the effect of Klotho rs3752472 polymorphism on the renal epithelium injury caused by CaOx. A rat urolithiasis model was established and validated. Renal function was assessed, and histological examination was performed. The distribution and expression of Klotho in the rat model were detected by immunohistochemical staining and western blotting analysis. A renal epithelial cell line (HK2) was used and intervened by COM crystals with several concentrations and time points. Expression of Klotho and key mediators in Wnt/ß-catenin pathway were assessed by Western blotting analysis. Wide-type and mutated plasmids of Klotho rs3752472 were added in the cell culture, and the activation of Wnt/ß-catenin signaling was tested. Finally, Wide-type and mutated plasmids of Klotho rs3752472 were adoptively transferred to the rat model, and the expression of Klotho was verified. In the rat model, Klotho was mainly distributed in the renal tubular area, which significantly declined in the urolithiasis group. In vitro, COM crystals significantly inhibited the expression of Klotho and induced remarkable renal epithelial cell injury. The mutation of Klotho rs3752472 can notably enhance the expression of Klotho, as well as the protection from renal epithelial cell injury and the inhibition of Wnt/ß-catenin signaling pathway. After adoptively transferred to the rat urolithiasis model, similar results were observed for the mutation of Klotho rs3752472. Klotho was significantly correlated with the renal epithelial cell injury induced by CaOx crystals. Furthermore, the mutation of Klotho rs3752472 can remarkably enhance the expression of Klotho in renal tissues and cells, and subsequently protect the renal epithelial cell from the formation of CaOx crystals through the inhibition of Wnt/ß-catenin signaling pathway.

7.
J Microbiol ; 59(7): 693-701, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33990912

RESUMO

Due to the different rates of diabetes in different ethnic groups and the structural differences in intestinal microbiota, this study evaluated the changes in diabetes-related intestinal microbiota in two ethnic groups. Fifty-six stool samples were collected from subjects from the Han and Mongolian ethnic groups in China, including participants without diabetes (non-diabetic, ND) and with type 2 diabetes (T2D). The 16S rDNA gene V3 + V4 area was extracted from microbiota, amplified by PCR, and used to perform high-throughput sequencing and screen differential microbiota associated with ethnicity. The results showed that there were 44 T2D-related bacterial markers in the Han subjects, of which Flavonifractor, Alistipes, Prevotella, Oscillibacter, Clostridium XlVa, and Lachnospiracea_incertae_sedis were most closely related to diabetes. There were 20 T2D-related bacterial markers in the Mongolian subjects, of which Fastidiosipila and Barnesiella were most closely related to diabetes. The common markers of T2D bacteria in the two ethnic groups were Papillibacter and Bifidobacterium. There were 17 metabolic pathways with significant differences between the ND and T2D groups in the Han group, and 29 metabolic pathways in the Mongolian group. The glutamatergic metabolic pathway was the only common metabolic pathway in two ethnic groups. The composition and function of diabetes-related bacteria were significantly different among the different ethnic groups, which suggested that the influence of ethnic differences should be fully considered when studying the association between diabetes and bacteria. In addition, the common bacterial markers found in diabetic patients of different ethnic groups in this study can be used as potential targets to study the pathogenesis and treatment of diabetes.

8.
J Mech Behav Biomed Mater ; 119: 104514, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33865066

RESUMO

Surface texturing is an effective approach to improve the tribological performance of artificial joints. In this paper, the frictional performance of Ultra-High-Molecular-Weight-Polyethylene and Cobalt-Chromium-Molybdenum material combination with micro grooves fabricated on the metal bearings is studied. The results show that grooves with width of 500 µm, depth of 4.5 µm and pitch distance of 3 mm could provide the optimized tribological performance, the coefficient of friction of which can be down to 0.05 showing a reduction of 51.9% compared to that of polished samples without micro grooves. A two-dimensional simulation of hydrodynamic pressure, based on Reynolds equation, is conducted. It is concluded that hydrodynamic pressure has little effect on the improved tribological performance of textured bioimplants. Otherwise, second lubrication effect induced by the polymer plastic deformation is proved to play a major role in the reduction of coefficient of friction.


Assuntos
Cromo , Cobalto , Fricção , Lubrificação , Teste de Materiais
9.
Shanghai Kou Qiang Yi Xue ; 30(1): 1-6, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33907770

RESUMO

Judged from the clinical manifestations, etiological mechanisms and jaw morphological structures, malocclusions with mandibular deviation and facial asymmetry are classified into three main categories, i.e., those with mandibular positioning factors, those with mandibular condyle defects, and those with jaw congenital deformities. In the first category, three subtypes, including mandibular rotation caused by dentitional irregularities, by dental compensation and by habitual posturing, are further defined. In the second category, mandibular deviation caused by condylar asymmetrical resorption or hyperplasia are defined as the two subtypes. The third category is further divided into two subtypes, i.e., jaw and facial asymmetry resulting from maxillary or mandibular structural deformities. The prime goal of orthodontic therapy is to displace and restore the deviated mandible to its right position, followed by correction of occlusal intercuspation. As an important procedure of the whole treatment protocol, It is critical to detect and localize the correct mandibular position by manipulation and then register it by wax pattern and articulator transferring. For the cases with mandibular functional elements and condylar asymmetrical length, the treatment modalities include orthopedic repositioning of the mandible by using occlusal and bite plane, SGTB and SGHB therapies; for cases with growth and developmental anomalies, orthognathic intervention should be an optimal solution.


Assuntos
Assimetria Facial , Má Oclusão , Oclusão Dentária , Humanos , Mandíbula , Côndilo Mandibular/diagnóstico por imagem
10.
J Cardiothorac Surg ; 16(1): 10, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33413551

RESUMO

BACKGROUND: As the positions and sizes of nodules in synchronous multiple primary lung cancer (SMPLC) patients differ, the development of surgical strategies to maximize long-term survival and preserved postoperative pulmonary function in SMPLC patients for whom surgical resection is an alternative strategy presents challenges. CASE PRESENTATION: We provide a case managed through video-assisted thoracoscopic surgery (VATS) resection using three-dimensional computed tomography lung reconstruction (3D-CTLR) to reconstruct lobes containing pulmonary nodules to preoperatively simulate and intraoperatively guide the extent and method of resection. CONCLUSION: The successful attempt demonstrates a technically simplified, feasible alternative to preoperative plans utilizing less invasive VATS to manage SMPLC.


Assuntos
Imageamento Tridimensional/métodos , Neoplasias Pulmonares/cirurgia , Nódulos Pulmonares Múltiplos/cirurgia , Neoplasias Primárias Múltiplas/cirurgia , Simulação por Computador , Feminino , Humanos , Neoplasias Pulmonares/diagnóstico por imagem , Pessoa de Meia-Idade , Nódulos Pulmonares Múltiplos/diagnóstico por imagem , Neoplasias Primárias Múltiplas/diagnóstico por imagem , Período Pré-Operatório , Estudos Retrospectivos , Cirurgia Assistida por Computador , Cirurgia Torácica Vídeoassistida , Tomografia Computadorizada por Raios X
11.
Int J Immunogenet ; 48(1): 8-15, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32996280

RESUMO

The present study aimed to analyse the frequencies of human leukocyte antigen HLA-ABCDQB1 and HLA-DRB1 alleles and haplotypes in a subset of 3,732 Han population from Hubei of China. All samples were typed in the HLA-ABCDQB1 and HLA-DRB1 loci using the sequence-based typing method; subsequently, the HLA polymorphisms were analysed. A total of 47 HLA-A, 89 HLA-B, 43 HLA-C, 49 HLA-DRB1 and 24 HLA-DQB1 alleles were identified in the Hubei Han population. The top three most frequent alleles in the HLA-ABCDQB1 and HLA-DRB1 were A*11:01 (0.2617), A*24:02 (0.1590), A*02:07 (0.1281); B*46:01 (0.1502), B*40:01 (0.1409) and B*58:01 (0.0616); C*01:02 (0.2023), C*07:02 (0.1691) and C*03:04 (0.1175); and DQB1*03:01 (0.2000), DQB1*03:03 (0.1900), DQB1*06:01 (0.1187); DRB1*09:01 (0.1790), DRB1*15:01 (0.1062) and DRB1*12:02 (0.0841), respectively. Meanwhile, the three most frequent two-loci haplotypes were A*02:07-C*01:02 (0.0929), B*46:01-C*01:02 (0.1366) and DQB1*03:03-DRB1*09:01 (0.1766). The three most frequent three-loci haplotypes were A*02:07-B*46:01-C*01:02 (0.0883), B*46:01-DQB1*03:03-DRB1*09:01 (0.0808) and C*01:02-DQB1*03:03-DRB1*09:01 (0.0837). The three most frequent four-loci haplotypes were A*02:07-B*46:01-C*01:02-DQB1*03:03 (0.0494), B*46:01-DRB1*09:01-C*01:02-DQB1*03:03 (0.0729) and A*02:07-B*46:01-DQB1*03:03-DRB1*09:01 (0.0501). The most frequent five-loci haplotype was A*02:07-B*46:01-C*01:02-DQB1*03:03-DRB1*09:01 (0.0487). Heat maps and multiple correspondence analysis based on the frequencies of HLA specificity indicated that the Hubei Han population might be described into Southern Chinese populations. Our results lay a certain foundation for future population studies, disease association studies and donor recruitment strategies.

12.
Medicine (Baltimore) ; 99(52): e23929, 2020 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-33350798

RESUMO

BACKGROUND: Several studies demonstrated a connection between human leukocyte antigen (HLA)-B∗1502 and lamotrigine (LTG)-induced cutaneous adverse drug reactions (cADRs). The correlation between the HLA-A∗24:02 and LTG-cADRs remains controversial. To examine the associations between HLA-A∗24:02 and LTG-cADRs, we conducted a systematic review and meta-analysis. METHODS: We performed a comprehensive search of the literature in several electronic database systems including Cochrane Library, EMBASE and PubMed from inception to January 2020. Review Manager was used to compare the frequencies of HLA-A∗24:02 carriers between the subgroups. RESULTS: A total of 5 studies were eligible, including 197 LTD-cADRs, 396 LTD-tolerant controls, and 2068 population controls. Compared with the LTG-tolerant controls, there was a statistically significant association between the HLA-A∗24:02 allele and LTG-induced cADRs (odds ratios: 1.94, 95% confidence intervals 1.06-3.54; P = .03). Compared with the general population, the relationship between the HLA-A∗24:02 genotype and LTG-induced cADRs was statistically significant (summary odds ratios: 2.12, 95% confidence intervals 1.04-4.30; P = .04). CONCLUSIONS: HLA-A∗24:02 may be a risk factor for LTG-cADRs.


Assuntos
Erupção por Droga/genética , Antígeno HLA-A24/genética , Lamotrigina/efeitos adversos , Anticonvulsivantes/efeitos adversos , Anticonvulsivantes/farmacologia , Humanos , Lamotrigina/farmacologia , Variantes Farmacogenômicos , Fatores de Risco
13.
Inorg Chem ; 59(24): 18109-18121, 2020 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-33241678

RESUMO

A series of phenylpyridine (ppy)-based 6/5/5 N*C^N^O and biphenyl (bp)-based 6/5/6 N*C^C*N Pt(II) complexes employing tetradentate ligands with nitrogen or oxygen atoms as bridging groups have been developed. Ligand structural modifications have great influences on the electrochemical, photophysical, and excited-state properties, as well as photostabilities of the Pt(II) complexes, which were systematically studied by experimental and theoretical investigations. The time-dependent density functional theory calculations and natural transition orbital analyses reveal that Pt(bp-6), Pt(bp-7), and Pt(bp-8) have dominant ligand-centered (3LC) mixed with small metal-to-ligand charge-transfer (3MLCT) characters in T1 states, resulting in relatively low quantum efficiencies (ΦPL) of 5-33% and 12-32% in dichloromethane solution and PMMA film, respectively. By contrast, Pt(ppy-1) possesses much more 3MLCT character in the T1 state, enabling a high ΦPL of 95% in dichloromethane and 90% in DPEPO film, and large radiative decay rates. The strength of the Pt-N1 coordination bond plays a critical role in the photostability. Pt(ppy-1)- and Pt(bp-6)-doped polystyrene films demonstrate long photostability lifetimes of 150 min for LT97 and LT98.5, respectively. A Pt(ppy-1)-based green OLED using 26mCPy as host realized a peak EQE of 18.5%, which still maintained an EQE of 10.4% at 1000 cd/m2, and an Lmax of over 40 000 cd/m2 was achieved. This study should provide a valuable reference for the further development of efficient and stable phosphorescent Pt(II) complexes.

14.
J Pak Med Assoc ; 70(10): 1727-1730, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33159742

RESUMO

OBJECTIVE: To investigate the clinical characteristics of early recurrent intussusception after ultrasound-guided saline reduction, and to explore the factors leading to early recurrence. Methods: The retrospective observational case-control study was conducted at Weifang People's Hospital, Shandong, China, and comprised data from January 2015 to December 2017 related to paediatric intussusception patients aged 0-12 years who underwent ultrasound-guided saline enema reduction. The patients were divided into two recurrent and non-recurrent groups. Clinical characteristics of the patients with early recurrence were analysed. Factors compared between the groups were gender, age, onset season, onset-to-treatment time interval, blood in stool, fever, diarrhoea, abdominal pain and vomiting, weight and pathology. Data was analysed using SPSS 22. RESULTS: Of the 672 subjects, 86(13%) were patient with early recurrence while 586(87%) had no early recurrence and acted as controls. Among the patients, 70(81.4%) were aged 6-36 months. In 52(60.5%) patients, recurrence was once, and in 23(26.7%) twice. There were 141 episodes of intussusception; 24(17%) occurring in <12 hours, 85(60.2%) in 12-24 hours. Also, 5(6%) patients required surgery for reduction. Compared to the controls, the second quarter, heavier body weight and pathology were the factors leading to early recurrence of intussusceptions (p<0.05). CONCLUSIONS: The second quarter, heavier body weight and pathological leading points were found to be factors leading to early recurrent intussusception.


Assuntos
Intussuscepção , Solução Salina , Estudos de Casos e Controles , Criança , Pré-Escolar , China , Enema , Humanos , Lactente , Recém-Nascido , Intussuscepção/diagnóstico por imagem , Intussuscepção/epidemiologia , Recidiva , Estudos Retrospectivos , Solução Salina/uso terapêutico , Resultado do Tratamento
15.
Front Pediatr ; 8: 535, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32984229

RESUMO

Barium sulfate is widely used for gastroenterology imaging. Retention of barium in the appendix, where it acts as an appendicolith, thereby leading to obstruction and inflammation. Barium-associated appendicitis is a very rare complication of upper gastrointestinal imaging (UGI), especially in children. We present a case involving an 8-year-old girl who was diagnosed with acute appendicitis due to a barolith and required a laparoscopic appendectomy 6 weeks after UGI. After UGI, patients should be informed regarding possible retention of barium in the appendix, which can cause acute appendicitis. Then, a laparoscopic appendectomy was performed successfully. We should be cautious of this potential risk to prevent complications with early intervention in children.

16.
Phys Rev E ; 102(2-1): 023204, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32942472

RESUMO

To study the effects of chunk mixing, the implosion experiments using capsules filled with deuterated foam (CDF capsule) were carried out on the Shenguang laser facility. Three types of the CDF capsules, namely the capsules without Au dopant, with micrometer Au dopant, and with atomic Au dopant, were used in the experiments. The neutron yields, the size, and the emission intensity of the hotspots were measured. The CDF capsules without Au dopant produced the highest neutron yield and the largest hotspot size at the time of peak emission. The capsules with micrometer or atomic Au dopant showed similar reduced neutron yield and hotspot size. The time-resolved hotspot emissions showed different behaviors between different capsules. One-dimensional simulations were carried out to understand the implosion dynamics of the CDF capsule without Au dopant, and to provide the thermodynamic conditions that the Au dopant would experience during the implosion. The effects of Au dopant were then discussed qualitatively.

17.
Exp Ther Med ; 20(4): 3174-3184, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32855686

RESUMO

Orthodontic-induced root resorption is a severe side effect that can lead to tooth root shortening and loss. Compressive force induces tissue stress in the cementum that covers the tooth root, which is associated with activation of bone metabolism and cementum resorption. To investigate the role of cementocytes in mechanotransduction and osteoclast differentiation, the present study established an in vitro three-dimensional (3D) model replicating cellular cementum and observed the effects of static compression on the cellular behavior of the cementocytes. Cell Counting Kit-8 assay, alkaline phosphatase staining and dentin matrix protein 1 quantification were used to evaluate the cementocyte differentiation in the 3D scaffolds. Cellular viability under static compression was evaluated using live/dead staining, and expression of mineral metabolism-related genes were analyzed via reverse transcription-quantitative PCR. The results suggested that the cementocytes maintained their phenotype and increased the expression of osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL) and sclerostin (SOST) in the 3D model compared with cells cultured in two dimensions. Compression force increased cell death and induced osteoclastic differentiation via the upregulation of SOST and RANKL/OPG ratio, and the downregulation of osteocalcin. The effect of compression showed a force magnitude-dependent pattern. The present study established an in vitro model of cellular cementum to study the biology of cementocytes. The results indicated that cementocytes are sensitive to mechanical loading and may serve potential roles in the metabolic regulation of minerals during orthodontic root resorption. These findings provide a novel tool to study biological processes in the field of orthodontics and expand knowledge of the biological function of cementocytes.

18.
Ital J Pediatr ; 46(1): 113, 2020 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-32758256

RESUMO

Hepatoblastoma (HB) is the most common malignant liver tumor in children. Abnormal activation of the Wnt/ß-catenin signaling pathway plays an important role in the formation and development of HB. Genes in HB show a global hypomethylation change, accompanied by hypermethylation of specific tumor suppressor genes (TSGs). This article reviews the hypermethylation changes in several TSGs, such as RASSF1A, SOCS1, APC, HHIP, and P16, and analyzes the pathways and mechanisms of TSGs regulating gene expression. The role of the methylation-regulating enzymes DNA methyltransferases (DNMTs) and ten-eleven translocation (TET) family members enzymes in the methylation changes of HB was analyzed, and it was speculated that the occurrence of HB is partly due to the obstruction of liver differentiation in the early stage of differentiation. The origin cells may be incompletely differentiated hepatocytes remaining in the liver of children after birth. Therefore, further studying the role of methylation regulating enzymes in methylation changes in HB is a promising future research direction.

19.
J BUON ; 25(2): 1148-1154, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32521919

RESUMO

PURPOSE: To investigate the influence of castration on insulin resistance, quality of life and immune function of prostate cancer (PCa) patients. METHODS: A total of 57 PCa patients definitely diagnosed via prostate biopsy underwent bilateral orchiectomy. No patient had history of diabetes mellitus before operation. The hemoglobin, leukocyte count, platelet count, albumin and alkaline phosphatase in the blood before operation and at 1 year after operation were analyzed using a full-automatic biochemistry analyzer, and the neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) in the peripheral blood were calculated. RESULTS: The levels of serum testosterone (T) and free testosterone (FT) in PCa patients declined remarkably at 1 month after castration. Compared with those before operation, the levels of serum T and FT were decreased significantly at 1, 2, 4 and 8 months as well as 1 year after castration. The levels of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were elevated gradually with the prolongation of time after operation. The level of high-density lipoprotein cholesterol (HDL-C) displayed an apparent rising trend from 2 months after surgical castration. The results of flow cytometry indicated that the levels of cluster of differentiation (CD) 4+ and CD4+/CD8+ were lowered markedly, while that of CD8+ was raised significantly in comparison with those before castration (p<0.05) After castration, both fasting blood glucose and fasting insulin were increased obviously in the patients (p<0.05). The 2 h postprandial blood glucose and insulin were raised distinctly at 1 month after castration (p<0.05). The insulin resistance index was increased persistently and prominently (p<0.05). CONCLUSION: The treatment of PCa through castration can aggravate the insulin resistance, reduce the immune function and improve the patient quality of life.


Assuntos
Castração/efeitos adversos , Imunidade/fisiologia , Resistência à Insulina/fisiologia , Neoplasias da Próstata/complicações , Neoplasias da Próstata/cirurgia , Qualidade de Vida/psicologia , Adulto , Idoso , Castração/métodos , Humanos , Masculino , Pessoa de Meia-Idade
20.
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