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1.
Zhongguo Zhong Yao Za Zhi ; 45(11): 2502-2508, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-32627481

RESUMO

In this study, the growth index including plant height, compound leaf area, specific leaf area, leaf water content, number of branches, and leaf biomass per plant and the icariin flavonoids such as epimedin A, epimedin B, epimedin C and icariin of Epimedium pseudowushanense were determined on 30 d and 60 d under light intensity(18.2±2.5) µmol·m~(-2)·s~(-1)(L1) and(90.9 ±2.5) µmol·m~(-2)·s~(-1)(L2), and white light as control, red light, blue light and yellow light were used as three light quality treatments, to study the effect of light quality on the growth and flavonoids accumulation of E. pseudowushanense. The E. pseudowushanense was sui-table for growth under L1 light intensity, the blue light treatment significantly reduced the leaf area, but had little effect on the stem height, the red light treatment and the yellow light treatment had no obvious effect on the stem height and leaf area, but the yellow light treatment significantly increased the germination of new branches, and had a sustained promoting effect, and the biomass was significantly higher than the white light treatment at 60 d. The content of icariin flavonoids in red light, blue light and yellow light treatment was higher than that in white light treatment at 30 d and 60 d under L1 light intensity, while yellow light treatment promoted the synthesis of icariin flavonoids to the largest extent, which was 1.8 and 1.9 times of white light treatment(30 d and 60 d).Under L2 light intensity, the effect of strong light on promoting stem germination became the main factor, while the yellow light treatment showed no significant effect on promoting stem germination, and the red light treatment exhibited a significant effect on reducing leaf area. Icariin flavonoids under red light, blue light and yellow light treatment were all lower than that under white light treatment, that is, the effect of white light treatment on the synthesis of icariin flavonoids is better than red light, blue light and yellow light treatment. When the time of strong light treatment was longer, the degradation range of icariin flavonoids in other light treatment appeared, while red light treatment promotes the synthesis of icariin flavonoids. Therefore, the influence of light quality on E. pseudowushanense is quite different under different light intensity, no matter from growth index or flavonoid content index. The results support that the biomass and icariin flavonoid content can be increased by providing appropriate red and yellow light.


Assuntos
Medicamentos de Ervas Chinesas , Epimedium , Flavonoides , Folhas de Planta
2.
J Chromatogr A ; 1620: 460999, 2020 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-32151418

RESUMO

Gas chromatography-mass spectrometry (GC-MS) is a robust analytical platform for analysis of small molecules. Recently, it is widely used for large-scale metabolomics studies, in which hundreds or even thousands of samples are analyzed simultaneously, producing a very large and complex GC-MS datasets. A number of software are currently available for processing GC-MS data, but it is too compute-intensive for them to efficiently and accurately align chromatographic peaks from thousands of samples. Here, we report a newly developed software, QPMASS, for analysis of large-scale GC-MS data. The parallel computing with an advanced dynamic programming approach is implemented in QPMASS to align peaks from multiple samples based on retention time and mass spectra, enabling fast processing large-scale datasets. Furthermore, the missing value filtering and backfilling are introduced into the program, greatly reducing false positive and false negative errors to be less than 5%. We demonstrated that it took only 8 h to align and quantify a GC-TOF-MS dataset from 300 rice leaves samples, and 17 h to process a GC-qMS dataset from 1000 rice seed samples by using a personal computer (3.70 GHz CPU, 16 GB of memory and > 100 GB hard disk). QPMASS is written in C++ programming language, and is able to run under Windows operation system with a user-friendly interface.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Metabolômica/métodos , Software , Algoritmos , Conjuntos de Dados como Assunto , Microcomputadores , Oryza/metabolismo
4.
J Biochem Mol Biol ; 39(2): 158-66, 2006 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-16584630

RESUMO

In many organisms, trehalose acts as protective metabolite against harsh environmental stresses, such as freezing, drought, nutrient starvation, heat and salt. Herein a cDNA (designated as GbTPS, GenBank Accession Number AY884150) encoding a trehalose-6-phosphate synthase homologue was isolated and characterized from the living fossil plant, Ginkgo biloba, which is highly tolerant to drought and cold. GbTPS encoded an 868-amino-acid polypeptide with a predicted isoelectric point of 5.83 and molecular mass of 97.9 kD. Amino acid sequence alignment revealed that GbTPS shared high identity with class II trehalose-6-phosphate synthase homologues (67% identical to AtTPS7), but had only 17% and 23% of identity with OstA from Escherichia coli and ScTPS1 from S. cerevisiae, respectively. DNA gel blot analysis indicated that GbTPS belonged to a small multi-gene family. The expression analysis by RT-PCR showed that GbTPS expressed in a tissue-specific manner in G. biloba and might involve in leaf development. GbTPS was also found to be induced by a variety of stresses including cold, salt, drought and mannitol.


Assuntos
Ginkgo biloba/enzimologia , Glucosiltransferases/química , Glucosiltransferases/genética , Sequência de Aminoácidos , Southern Blotting , Clonagem Molecular , Temperatura Baixa , DNA Complementar/química , Perfilação da Expressão Gênica , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Ginkgo biloba/efeitos dos fármacos , Ginkgo biloba/crescimento & desenvolvimento , Glucosiltransferases/efeitos dos fármacos , Manitol/farmacologia , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sementes/efeitos dos fármacos , Sementes/enzimologia , Sementes/crescimento & desenvolvimento , Homologia de Sequência de Aminoácidos , Cloreto de Sódio/farmacologia
5.
J Plant Physiol ; 163(2): 224-7, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16399014

RESUMO

We isolated a putative anthocyanidin reductase gene from Ginkgo biloba (designated as GbANR) by rapid amplification of cDNA ends (RACE). The full-length cDNA of GbANR is 1451 bp long with poly(A) tail and contains a 1026 bp open reading frame (ORF) encoding 342 amino acids. The GbANR gene is composed of five exons and four introns. Sequence alignment shows that the deduced GbANR has a relatively high identity to other plant anthocyanidin reductases at the amino acid level. DNA gel blot analysis indicates that GbANR belongs to a small gene family. Transcription analysis indicates that GbANR is preferentially expressed in leaves.


Assuntos
Genes de Plantas , Ginkgo biloba/genética , NADH NADPH Oxirredutases/genética , Sequência de Aminoácidos , Sequência de Bases , Dados de Sequência Molecular , Alinhamento de Sequência
6.
Biosci Rep ; 25(5-6): 345-62, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16307381

RESUMO

A novel agglutinin gene was cloned from Arisaema lobatum using SMART RACE-PCR technology. The full-length cDNA of Arisaema lobatum agglutinin (ala) was 1078 bp and contained a 774 bp open reading frame encoding a lectin precursor (proproprotein) of 258 amino acid residues with a 23 aa signal peptide. ALA contained three mannose-binding sites (QXDXNXVXY) with two-conserved domains of 45% identity, ALA-DOM1 and ALA-DOM2. The three-dimensional structure of ALA was very similar to that of GNA (Galanthus nivalis agglutinin). ALA shared varying identities, ranging from 40% to 85%, with mannose-binding lectins from other species of plant families, such as Araceae, Alliaceae, Iridaceae, Lillaceae, Amaryllidaceae and Bromeliaceae. Genomic sequence of ala was also cloned using genomic walker technology, and it was found to contain three putative TATA boxes and eight possible CAAT boxes in the 5'-flanking region. No intron was found within the region of genomic sequence. Southern blot analysis indicated that the ala belonged to a multi-copy gene family. Expression pattern analysis revealed that the ala preferentially expressed in the tissues with the higher expression being found in spadix, bud, leaf, spathe and tuber. The cloning of the ala gene not only provides a basis for further investigation of its structure, expression and regulation mechanism, but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into plants in the future.


Assuntos
Aglutininas/genética , Arisaema/metabolismo , Aglutininas/química , Sequência de Aminoácidos , Sequência de Bases , Sítios de Ligação , Southern Blotting , Clonagem Molecular , DNA/química , Primers do DNA/química , DNA Complementar/metabolismo , Evolução Molecular , Regulação da Expressão Gênica de Plantas , Biblioteca Gênica , Genes de Plantas , Genoma de Planta , Lectinas/química , Manose/química , Modelos Moleculares , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Reação em Cadeia da Polimerase , Sinais Direcionadores de Proteínas , Estrutura Terciária de Proteína , RNA/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos
7.
DNA Seq ; 15(4): 283-90, 2004 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-15620216

RESUMO

A chalcone synthase (CHS) gene was cloned from Ginkgo biloba for the first time and it was also the first cloned gene involved in flavonoids metabolic pathway in G. biloba. The full-length cDNA of G. biloba CHS (designated as Gbchs) was 1608bp with poly(A) tailing and it contained a 1173bp open reading frame (ORF) encoding a 391 amino acid protein. Gbchs was found to have extensive homology with those of other plant chs genes via multiple alignments. The active sites of the CoA binding, coumaroyl pocket and cyclization pocket in CHS protein of Medicago sativa were also found in GbCHS. Molecular modeling of GbCHS indicated that the three-dimensional structure of GbCHS strongly resembled that of M. sativa (MsCHS2), implying GbCHS may have similar functions with MsCHS2. Phylogenetic tree analysis revealed that GbCHS had closer relationship with CHSs from gymnosperm plants than from other plants. Gbchs is a useful tool to study the regulation of flavonoids metabolism in G. biloba.


Assuntos
Aciltransferases/genética , Ginkgo biloba/genética , Aciltransferases/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA Complementar , Evolução Molecular , Ginkgo biloba/enzimologia , Ginkgo biloba/metabolismo , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Análise de Sequência de DNA
8.
Yi Chuan ; 26(6): 893-7, 2004 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-15640122

RESUMO

An EST related to the gene PCD was isolated from SSH (suppression subtractive hybridization) library of callus tissues of rice (Oryza sativa L.). Primers were designed to obtain its complete cDNA encoding putative apoptosis-related protein from Shanyou 63 (Oryza sativa L.). Sequencing indicated that the gene contained a 387 bp open reading frame, which encodes a protein containing 128 aa. Sequence alignment showed that the deduced protein is highly homologous to the known PDCD5. Real time quantitative PCR was performed to reveal that rPDCD5 was up-regulated in abiotic stress (low temperature and NaCl treatment).


Assuntos
Proteínas Reguladoras de Apoptose/genética , Apoptose/genética , Oryza/genética , Proteínas de Plantas/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Temperatura Baixa , DNA Complementar/química , DNA Complementar/genética , Etiquetas de Sequências Expressas , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Dados de Sequência Molecular , Hibridização de Ácido Nucleico/métodos , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Cloreto de Sódio/farmacologia
9.
DNA Seq ; 14(4): 285-93, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-14640074

RESUMO

A novel osClpD gene, encoding a highly conservative ClpD subfamily member, was first isolated and characterized from Oryza sativa. The full-length cDNA of osClpD gene was 3140 bp and contained a 2884 bp open reading frame encoding a 938 amino acid protein. The phylogenetic tree and blast search showed that OSClpD belonged to the ClpD subfamily of the Hsp100/Clp family, and contained all protein motifs characteristic for the ClpD subfamily of Hsp100/Clp proteins. The real-time quantitative PCR analysis proved that it was inducible by water deficit and temperature stress in vegetative tissues.


Assuntos
Adenosina Trifosfatases/genética , Oryza/genética , Filogenia , Serina Endopeptidases/genética , Sequência de Aminoácidos , Sequência de Bases , Análise por Conglomerados , Primers do DNA , DNA Complementar/genética , Dessecação , Endopeptidase Clp , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Temperatura
10.
DNA Seq ; 14(4): 295-301, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-14631652

RESUMO

A subtractive cDNA library was constructed using rice (Oryza sativa L.) callus cDNA as driver and differentiating callus cDNA as tester. A novel cDNA fragment encoding RNase L inhibitor (RLI) was isolated by screening the subtractive library, which had a higher expression level in differentiating callus than in callus. The full-length cDNA of rice-RLI was obtained by the method of rapid amplification of cDNA ends, which contained a 1812-bp open reading frame encoding a 604 amino acid polypeptide. Homologous analysis showed that rice-RLI contained the conserved motifs (two repeated P-loops, two ATP-binding boxes and an iron-sulfur binding motif). The fluorescence quantitative PCR analysis showed that it was a constitutive expressed gene but up-regulated in abiotic stress (low temperature and NaCl treatment) and down-regulated under the treatments of NAA and IAA.


Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Chaperoninas/genética , Regulação da Expressão Gênica , Oryza/genética , Motivos de Aminoácidos , Sequência de Aminoácidos , Sequência de Bases , Primers do DNA , DNA Complementar/genética , Fluorescência , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Cloreto de Sódio , Temperatura , Fatores de Tempo
11.
DNA Seq ; 14(3): 163-7, 2003 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-14509828

RESUMO

A new lectin gene was cloned from Zephyranthes candida by using RACE-PCR. The full-length cDNA of Zephyranthes candida agglutinin (ZCA) was 647 bp and contained a 477 bp open reading frame encoding a 159 amino acid protein. Zephyranthes candida lectin gene was found to encode a precursor lectin with signal peptide and had extensive homology with those of other plant lectins. Molecular modeling of ZCA indicated that the three-dimensional structure of ZCA strongly resembles that of the snowdrop lectin, implying ZCA may have the similar insecticidal functions with GNA.


Assuntos
Lectinas/genética , Liliaceae/genética , Modelos Moleculares , Sequência de Aminoácidos , Sequência de Bases , Primers do DNA , DNA Complementar/genética , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência
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