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1.
Food Chem ; 335: 127647, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32739816

RESUMO

The inhibition mechanism of the texture deterioration of tilapia fillets after treatment with polyphenols during partial freezing for 49 days was studied. Carnosic acid (CA), procyanidin (PA), quercetin (QE), and resveratrol (RSV) treatments had significantly higher hardness values (over 230 g) than the control group (183 g) on day 49 (P < 0.05). Polyphenol treatments were effective in delaying the protein degradation, lipid oxidation and spoilage microbe growth. Moreover, the kinetic model showed that the predicted shelf life of tilapia fillets treated with PA (102 d) was extended by 25 d compared to the control group (77 d). It was the proposed possible mechanism that polyphenols comprehensively maintained the protein conformation (increased hydrogen bonds and decreased disulfide bonds) and retarded protein denaturation and degradation, protecting the texture of the fillets. Therefore, polyphenols can be used to maintain texture and extend the shelf life of tilapia fillets during partial freezing.


Assuntos
Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Carne/análise , Polifenóis/análise , Polifenóis/farmacologia , Animais , Biflavonoides/química , Catequina/química , Proteínas de Peixes/química , Conservação de Alimentos/instrumentação , Congelamento , Oxirredução/efeitos dos fármacos , Proantocianidinas/química , Desnaturação Proteica/efeitos dos fármacos , Tilápia
2.
J Texture Stud ; 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33230818

RESUMO

In order to improve the gel performance and edible quality of surimi for sustainable manufacture, the present study explored the feasibility of four kinds of starchs (potato, corn, sweet potato and wheat) and fresh water fish tilapia as additives for golden pompano based surimi production. Sweet potato starch stood out as the most appropriate additive for golden pompano surimi due to the highest gel strength, lowest expressible moisture content and more compact microstructure. When the tilapia flesh substitution ratio reached 30%, best gel property of tilapia-golden pompano compound surimi with best texture property and whiteness value was achieved. Moreover, addition of 5% sweet potato starch to the golden pompano-tilapia (7:3) compound surimi comparatively obtained the optimal effect. Namely, it was observed that the texture parameters with hardness (3.62 N), gumminess (2.74 N), chewiness (17.35 mJ), cohesiveness (4.918) and springiness (0.872) being biggest values. On the other hand, the gel strength and expressible moisture content were 2137.31 g. mm and 3.52%, respectively, which were overwhelming than other levels. Simultaneously, the whiteness of 5% addition group was 74.75, which was also a little higher than other groups. In summary, partial substitution of tilapia and proper addition of sweet potato starch effectively improved the gel performance and quality of golden pompano based surimi products, which has potential applications in the industry of surimi. This article is protected by copyright. All rights reserved.

3.
J Sci Food Agric ; 2020 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-32888322

RESUMO

BACKGROUND: The growing consumer demand for healthy products has encouraged the development of low-salt meat products. In this study, to develop low-salt restructured tilapia (Oreochromis mossambicus) meat products, citric acid was used to improve the properties of restructured tilapia products. RESULTS: In comparison with control restructured fish products (RP) and surimi products (SP), 0.2% citric acid-treated restructured fish products (RPC) and surimi products (SPC) showed a significant decrease in expressible water and water activity and a remarkable increase in whiteness, dry matter, hardness, chewiness, gumminess, and acceptability. Mechanistic studies suggested that citric acid significantly changed the content of total protein and myofibrillar proteins and promoted degradation of heavy myosin chains. Fourier-transform infrared and Raman spectra revealed the citric acid-mediated alteration in the peak intensities of amide I and amide II bands, which changed the secondary structures of RPC and SPC. CONCLUSION: It is feasible to prepare low-salt restructured tilapia meat products using citric acid, which offers a means of using muscle by-products and exploiting new functional products with an added commercial value. © 2020 Society of Chemical Industry.

4.
Food Res Int ; 133: 109138, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32466915

RESUMO

Round scad (Decapterus maruadsi) is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, there is no comprehensive information covering its lipidomic profile. In this study, lipid profiles including fatty acid composition, distribution and detailed structure information were explored using gas chromatography-flame ionization detector (GC-FID) and ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). The results showed that triacylglycerol (TG) was the dominant class, and the EPA and DHA were concentrated as glycerophospholipid (GP). The saturated fatty acids and monounsaturated fatty acids were mainly esterified in positions 1 and 3 of TG and sn-1-position of GP, while the polyunsaturated fatty acids (PUFA) were mainly esterified in sn-2-position of TG and GP. A total of 1282 species from six classes (21 subclasses) including glycerolipids (GL), sphingolipids (SP), GP, fatty acyls (FA), saccharolipids (SL) and prenol lipids (PR) were identified. Several molecular species were characterized with PUFA, especially EPA and DHA in GP. Considering the superior fatty acids composition and distribution of round scad, it is deserved for further exploitation of its marine lipid source on account of the healthy and nutritional functions.

5.
Food Funct ; 11(4): 3245-3255, 2020 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-32219260

RESUMO

In this study, we evaluated the effects of tilapia head glycolipids (TH-GLs) on male C57BL/6 mice with inflammatory bowel disease (IBD) induced by dextran sulfate sodium (DSS) and the changes in gut microbiota compared with sulfasalazine. Mice were orally treated with 3% (w/v) DSS or not for 7 days, followed by drug treatment with TH-GLs or sulfasalazine. After treatment, macroscopic colitis symptoms, intestinal epithelial barrier function, inflammatory cytokines, and gut microbiota homeostasis were assessed. Further studies showed that TH-GLs and sulfasalazine showed different influences on the gut microbiota structure. Both sulfasalazine and TH-GLs decreased the DSS-induced enrichment of Gammaproteobacteria and Enterobacteriaceae. However, TH-GLs had a selective increase in the enrichment of Akkermansia, Prevotellaceae, Oscillospira, Allobaculum, Bifidobacterium, and Coprococcus in contrast to sulfasalazine, which selectively increased the enrichment of Dorea, Turicibacter, Bacteroides, Coprobacillus, Mucispirillum, and Dehalobacterium. In addition, both TH-GLs and sulfasalazine relieved body weight loss, and increased the immune organ index, while maintaining the balance between pro-inflammatory and anti-inflammatory cytokines. The results indicate that TH-GLs alleviate DSS-induced IBD in mice by decreasing the abundance of harmful gut microbiota and enhancing the abundance of probiotic gut microbiota. Thus, the mechanism through which TH-GLs inhibit inflammation through gut microbiota is different from that of sulfasalazine. Therefore, TH-GLs stand as potential prebiotics for the treatment of colonic inflammation and related diseases.

6.
J Agric Food Chem ; 68(6): 1621-1633, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-31967468

RESUMO

Collagen peptides can promote wound healing and are closely related to microbiome colonization. We investigated the relationship among collagen peptides, wound healing, and wound microflora colonization by administering the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Tilapia nilotica skin collagen peptides (Tn-SCPs). We analyzed the vascular endothelial growth factor (VEGF), fibroblast growth factors (ß-FGF), pattern recognition receptor (NOD2), antimicrobial peptides (ß-defence14, BD14), proinflammatory (TNF-α, IL-6, and IL-8) and anti-inflammatory (IL-10) cytokines, macrophages, neutrophil infiltration levels, and microbial communities in the rat wound. The healing rates of the Ss-SCP- and Tn-SCP-treated groups were significantly accelerated, associated with decreased TNF-α, IL-6, and IL-8 and upregulated BD14, NOD2, IL-10, VEGF, and ß-FGF. Accelerated healing in the collagen peptide group shows that the wound microflora such as Leuconostoc, Enterococcus, and Bacillus have a positive effect on wound healing (P < 0.01). Other microbiome species such as Stenotrophomonas, Bradyrhizobium, Sphingomonas, and Phyllobacterium had a negative influence and decreased colonization (P < 0.01). Altogether, these studies show that collagen peptide could upregulate wound NOD2 and BD14, which were implicated in microflora colonization regulation in the wound tissue and promoted wound healing by controlling the inflammatory reaction and increasing wound angiogenesis and collagen deposition.


Assuntos
Colágeno/química , Proteínas de Peixes/química , Microbiota/efeitos dos fármacos , Proteína Adaptadora de Sinalização NOD2/genética , Peptídeos/administração & dosagem , Pele/química , Ferimentos e Lesões/fisiopatologia , beta-Defensinas/genética , Administração Cutânea , Animais , Bactérias/classificação , Bactérias/efeitos dos fármacos , Bactérias/genética , Bactérias/isolamento & purificação , Ciclídeos , Fatores de Crescimento de Fibroblastos/genética , Fatores de Crescimento de Fibroblastos/imunologia , Humanos , Interleucina-10/genética , Interleucina-10/imunologia , Masculino , Camundongos , Proteína Adaptadora de Sinalização NOD2/imunologia , Peptídeos/química , Ratos , Ratos Sprague-Dawley , Salmo salar , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/imunologia , Cicatrização/efeitos dos fármacos , Ferimentos e Lesões/tratamento farmacológico , Ferimentos e Lesões/imunologia , Ferimentos e Lesões/microbiologia , beta-Defensinas/imunologia
7.
Food Chem Toxicol ; 135: 110886, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31626838

RESUMO

Diabetes mellitus has become a worldwide concern in recent years. In this study, the effect of Holothuria leucospilota polysaccharide (HLP) on type 2 diabetes mellitus (T2DM) was investigated in Goto-Kakizaki (GK) rats. The results showed that HLP significantly improved glucose intolerance and regulated blood lipid and hormone levels (p < 0.05). Pathological analysis showed that HLP repaired the impairments of the pancreas and colon in diabetic rats. In addition, a high dose of HLP (200 mg/kg) significantly upregulated the gene expression of peroxisome proliferator-activated receptor-α (PPAR-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), phosphoinositide 3-kinase (PI3K), protein kinase B (PKB/AKT), glucose transporter-4 (GLUT4) and anti-apoptotic (Bcl-2), and downregulated the mRNA levels of pro-apoptotic (Bax) and cluster of differentiation 36 (CD36) in diabetic rats (p < 0.05). Furthermore, HLP treatment increased the short-chain fatty acid-producing bacteria and decreased the opportunistic bacterial pathogen in the feces of diabetic rats. These results demonstrated that HLP has the potential to ameliorate T2DM in GK rats.


Assuntos
Diabetes Mellitus Experimental/microbiologia , Diabetes Mellitus Tipo 2/microbiologia , Microbioma Gastrointestinal/efeitos dos fármacos , Polissacarídeos/farmacologia , Adiponectina/metabolismo , Animais , Glicemia/metabolismo , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Tipo 2/sangue , Peptídeo 1 Semelhante ao Glucagon/metabolismo , Insulina/metabolismo , Leptina/metabolismo , Lipídeos/sangue , Masculino , Receptores Ativados por Proliferador de Peroxissomo/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Pepinos-do-Mar , Transdução de Sinais
8.
Food Chem ; 305: 125454, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505413

RESUMO

The effects of the (-)-Epigallocatechin gallate (EGCG)-gelatin biofilm treatment (EGT) on microbial composition and quality of tilapia fillets stored at low temperatures were evaluated. The changes in mechanical properties, microbial reproduction, as well as lipid and protein oxidation during fillets storage were determined. The results showed that EGT reduced the microbial count and the relative abundance of the fillets. And EGT delayed the rate of lipid oxidation and protein denaturation in fillets. Compared with the control group, EGT samples had lower K values (74% on 18 d) and biogenic amines (39 mg/kg for putrescine and 50 mg/kg for cadaverine on 21 d). According to sensory evaluation, the shelf life of tilapia fillets was extended by 6 d in the EGT group. Therefore, EGT improved the quality of cryopreserved tilapia fillets and could be considered as a potential method for fish fillet preservation.


Assuntos
Materiais Biomiméticos/química , Catequina/análogos & derivados , Armazenamento de Alimentos/métodos , Gelatina/química , Alimentos Marinhos/análise , Aeromonas/efeitos dos fármacos , Animais , Aminas Biogênicas/análise , Materiais Biomiméticos/farmacologia , Catequina/química , Peroxidação de Lipídeos/efeitos dos fármacos , Desnaturação Proteica/efeitos dos fármacos , Proteínas/química , Pseudomonas/efeitos dos fármacos , Alimentos Marinhos/microbiologia , Paladar/efeitos dos fármacos , Temperatura
9.
J Oleo Sci ; 68(12): 1187-1197, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31735749

RESUMO

In this study, we first isolate phospholipid (PL) from Golden pompano head (GPH), and elucidate its structure. Gas chromatography (GC) was used to assess the GPH-PL fatty acid composition, Fourier transform infrared spectroscopy (FTIR) and ultraviolet absorption spectrometry (UV) were used for the qualitative analysis of GPH-PL, and LC-MS analysis was used to determine the major PL species. The results show that the contents of the various molecular species of GPH-PL were generally in the order phosphatidylcholine (PC) > sphingomyelin (SM) > lysophosphatidylcholine (LPC) > phosphatidylethanolamine (PE). The main molecular PC species are 16:0/18:2, 13:0/23:2, 27:2/9:0, 16:0/18:1, 12:0/22:2, 18:0/18:1, 18:0/24:1, and 18:1/24:0. The major SM species are 16:1/16:0, 16:0/18:1, 16:0/18:2, 16:0/26:2, and 18:1/24:1. The major LPC species are 18:1 and 16:0. The major PE species are 18:0/18:1 and 16:0/22:6. The total eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents in the GPH-PLs were 18.39%, and the content of DHA in the PL fraction was 16.47%. These results suggest that PLs from GPH is rich in polyunsaturated fatty acids (PUFA), which have good activity in anti-inflammation, anti-tumor, anti-osteoporosis and other aspects, and have important development prospects in the future.


Assuntos
Fosfolipídeos/análise , Animais , Cromatografia Líquida , Peixes , Cabeça , Espectrometria de Massas , Estrutura Molecular , Fosfolipídeos/química , Fosfolipídeos/isolamento & purificação
10.
Int J Mol Sci ; 20(19)2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31554265

RESUMO

Holothuria leucospilota polysaccharides (HLP) are expected to become potential resources for the treatment of hyperlipidemia because of their various bioactivities. In the study, the treatment of HLP on improving hyperlipidemia in rats was explored. Oral administration of HLP at 100 or 200 mg/kg body weight effectively alleviated serum lipid levels and liver histological abnormalities in high-fat-diet rats. HLP regulated abnormal mRNA, lipogenesis-related hormones and inflammatory cytokines (tumor necrosis factor-α, interleukin-6 and interleukin-12) levels. HLP improved the ability of gut microbiota to produce short-chain fatty acids (SCFAs). SCFAs have been found to ameliorate liver lesions. Therefore, HLP alleviated hyperlipidemia by improving the levels of SCFAs to regulate lipid metabolism. These results indicated that HLP could be used as beneficial polysaccharides to alleviate hyperlipidemia.


Assuntos
Dieta Hiperlipídica/efeitos adversos , Ácidos Graxos Voláteis/metabolismo , Holothuria/química , Hiperlipidemias/etiologia , Hiperlipidemias/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Polissacarídeos/farmacologia , Animais , Modelos Animais de Doenças , Microbioma Gastrointestinal , Hormônios/metabolismo , Hiperlipidemias/tratamento farmacológico , Mediadores da Inflamação , Lipídeos/sangue , Fígado/metabolismo , Fígado/patologia , Modelos Biológicos , Polissacarídeos/química , Ratos
11.
Food Res Int ; 123: 217-225, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31284971

RESUMO

The impacts of the vacuum freeze (VFD), hot air (HAD), microwave (MD) and vacuum microwave (VMD) drying on the flavour of golden pompano fillets were evaluated using an electronic nose (E-nose), an electronic tongue (E-tongue) and simultaneous distillation extraction (SDE) - gas chromatography - mass spectrometry (GC-MS). The results showed that the E-nose and E-tongue systems could effectively differentiate volatile compounds of four samples. A total of 86 volatile flavour components were identified in the dried fillets; the main flavour components contained hydrocarbons (39), aldehydes (15), esters (10) and alcohols (9). HAD, MD and VMD processing promoted a gradual reduction in ketones and the generation of esters, while the fillets that were processed by VFD contained more hydrocarbon (29.68%) and alcohol (2.64%) compounds. The volatile compounds of dried golden pompano fillets were developed through four potential pathways, including the Maillard reaction, lipid oxidation and degradation, protein hydrolysis, and Strecker degradation.

12.
Food Chem ; 271: 204-210, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30236668

RESUMO

The effects of (-)-epigallocatechin gallate (EGCG) on the mechanical, antioxidative, antimicrobial, cross-linking and release properties of gelatin film were evaluated. The biofilm exhibited excellent antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli in the presence of EGCG at more than 3%. With the increasing content of EGCG (1%-5%), higher denaturation temperature (66.5-84.6 °C) and bloom strength (609.3-1114.7 g) and denser microstructure suggested the existence of cross-linking bonds in the biofilm. The infrared spectroscopy, protein pattern and release property of EGCG showed that hydrogen bonds were the main driving force for cross-linking of the biofilm. The release of EGCG from the biofilm system was described and two compounds were cross-linked via hydrogen bonds. Thus, this study confirmed the improvement on mechanical, bioactivities and release properties of the biofilm was most likely due to the interactions between gelatin and EGCG.


Assuntos
Catequina/química , Gelatina/química , Antioxidantes , Biofilmes
13.
Molecules ; 25(1)2019 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-31906039

RESUMO

Gadus morhua eggs contain several nutrients, including polyunsaturated fatty acids, lecithin and glycoproteins. A novel sialoglycopeptide from the eggs of G. morhua (Gm-SGPP) was extracted with 90% phenol and purified by Q Sepharose Fast Flow (QFF) ion exchange chromatography, followed by S-300 gel filtration chromatography. Gm-SGPP contained 63.7% carbohydrate, 16.2% protein and 18.6% N-acetylneuraminic acid. High-performance size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that Gm-SGPP is a 7000-Da pure sialoglycopeptide. ß-elimination reaction suggested that Gm-SGPP contained N-glycan units. Amino acid N-terminal sequence analysis indicated the presence of Ala-Ser-Asn-Gly-Thr-Gln-Ala-Pro amino acid sequence. Moreover, N-glycan was connected at the third Asn location of the peptide chain through GlcNAc. Gm-SGPP was composed of D-mannose, D-glucuronic acid and D-galactose. Fourier transform-infrared spectroscopy (FT-IR), 1H-nuclear magnetic resonance spectroscopy (1H-NMR) and methylation analysis were performed to reveal the structure profile of Gm-SGPP. In vitro results showed that the proliferation activity of MC3T3-E1 cells was significantly promoted by Gm-SGPP. In vivo data revealed that Gm-SGPP increased the calcium and phosphorus content of tibias and promoted longitudinal bone growth in adolescent rats.


Assuntos
Gadus morhua/metabolismo , Osteogênese/efeitos dos fármacos , Óvulo/química , Sialoglicoproteínas/farmacologia , Tíbia/crescimento & desenvolvimento , Motivos de Aminoácidos , Animais , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Cromatografia em Gel , Cromatografia por Troca Iônica , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/farmacologia , Camundongos , Peso Molecular , Fósforo/análise , Ratos , Sialoglicoproteínas/química , Sialoglicoproteínas/genética , Espectroscopia de Infravermelho com Transformada de Fourier , Tíbia/química , Tíbia/efeitos dos fármacos
14.
J Oleo Sci ; 67(4): 407-417, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29526879

RESUMO

Human milk fat substitutes (HMFS) are rich in polyunsaturated fatty acids which upon microencapsulation, can be used as a source of high quality lipids in infant formula. The response surface methodology (RSM) was employed to optimize the microencapsulation condition of HMFS as a functional product. The microencapsulation efficiency (MEE) of microencapsulated HMFS was investigated with respect to four variables including concentration of soy lecithin (A), ratio of demineralized whey powder to malt dextrin (B), HFMS concentration (C), and homogenizing pressure (D). The optimum conditions for efficient microencapsulation of HMFS by the spray drying technique were determined as follows: the amount of soybean lecithin-0.96%, ratio of desalted whey powder to malt dextrin-2.04:1, oil content-17.37% and homogeneous pressure-0.46MPa. Under these conditions, the MEE was 84.72%, and the basic indices of the microcapsules were good. The structure of the microcapsules, as observed by scanning electron microscopy (SEM), revealed spherical, smooth-surfaced capsules with diameters ranging between 10-50 µm. Compared with HFMS, the peroxide value (POV) and acid value (AV) of the microcapsule were significantly lower during storage indicating that the microencapsulation process increases stability and shelf life. Infrared spectroscopic analyses indicated that HFMS had the same characteristic functional groups as the oil extracted from microcapsules. Simulated in vitro digestion revealed that the microcapsules were digested completely within 2h with maximum lipid absorption rate of 64%. Furthermore, these results advocate the embedding process of HFMS by RSM due to its efficacy.


Assuntos
Gorduras Insaturadas na Dieta , Composição de Medicamentos/métodos , Ácidos Graxos Insaturados , Fórmulas Infantis/química , Leite Humano/química , Cápsulas , Dextrinas , Armazenamento de Alimentos , Humanos , Lactente , Lecitinas , Microscopia Eletrônica de Varredura , Tamanho da Partícula , Peróxidos/análise , Pós , Soja , Espectrofotometria Infravermelho , Soro do Leite
15.
J Oleo Sci ; 64(12): 1273-81, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26582156

RESUMO

The present study attempts to investigate the anti-inflammatory potential of the isolated lipid extracts of three-spot seahorse which is rare marine bony fish. Petroleum ether (PE) extract was obtained from systematic solvent extraction after reflux extraction with 95% ethanol. FrIV was collected after silica gel column chromatography, and neutral lipids (NL), glycolipids (GL), phospholipids (PL) were separated from FrIV. Basic compositions were detected and analyzed via thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). Anti-inflammatory activities of total lipids (TL), isolated NL, GL, and PL were detected by secretion of pro-inflammatory cytokines induced by lipopolysaccharide (LPS) in murine monocyte macrophage RAW264.7 cells in vitro. The results revealed that lipids of seahorse showed a positive correlation with the in vitro suppression of the release of nitric oxide (NO), interleukin (IL)-6, IL-1ß and tumor necrosis factor (TNF)-α potently in a dose dependent manner, and showed cell compatibility. Among the fractions, GL (50 µg/mL) showed the highest capacity to attenuate the generation of pro-inflammatory cytokines which was comparable to that of the positive drug dexamethasone (DX) (20 µg/mL). Collectively, our findings indicated that the lipids from seahorse may be effective in the management of inflammation.


Assuntos
Anti-Inflamatórios/farmacologia , Citocinas/metabolismo , Mediadores da Inflamação/metabolismo , Lipídeos/isolamento & purificação , Lipídeos/farmacologia , Smegmamorpha/metabolismo , Animais , Dexametasona/farmacologia , Relação Dose-Resposta a Droga , Glicolipídeos/isolamento & purificação , Glicolipídeos/farmacologia , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Lipopolissacarídeos/farmacologia , Extração Líquido-Líquido/métodos , Camundongos , Óxido Nítrico/metabolismo , Fosfolipídeos/isolamento & purificação , Fosfolipídeos/farmacologia , Células RAW 264.7 , Extratos de Tecidos/isolamento & purificação , Extratos de Tecidos/farmacologia , Fator de Necrose Tumoral alfa/metabolismo
16.
J Oleo Sci ; 64(9): 933-42, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26250427

RESUMO

Three-Spot seahorse is a traditional medicine in Asian countries. However, the alcohol extract is largely unknown for its anti-inflammatory activity. This study aimed at elucidating fraction of potent anti-inflammatory activity of seahorse. A systematic solvent extraction method of liquid-liquid fractionation of ethanol crude extract gave four fractions petroleum ether (PE), and ethyl acetate (EtOAc), water saturated butanol (n-BuOH), water (H2O). In this study, PE extract was selected for further study after preliminary screening test, and was connected to silica column chromatography and eluted with different polarity of mobile phases, and obtained four active fractions (Fr I, Fr II, Fr III, Fr IV). Effect of separated fractions on inflammation was investigated in lipopolysaccharide (LPS) stimulated murine RAW264.7 cells in vitro. The result shows that seahorse extract was capable of inhibiting the production of nitric oxide (NO) significantly in a dose dependent manner and exhibited no notable cytotoxicity on cell viability. IC50 of fraction IV was 36.31 µg/mL, indicating that separated fraction possessed potent NO inhibitory activity against LPS-induced inflammatory response, thus, demonstrated its in vitro anti-inflammatory potentiality, it may be at least partially explained by the presence of anti-inflammation active substances, phenolic compounds, phospholipids and polyunsaturated fatty acids, especially phospholipids and polyunsaturated fatty acids. It could be suggested that seahorse lipid-soluble components could be used in functional food and anti-inflammatory drug preparations.


Assuntos
Citocinas/metabolismo , Mediadores da Inflamação/metabolismo , Lipopolissacarídeos/efeitos adversos , Macrófagos/metabolismo , Óxido Nítrico/metabolismo , Smegmamorpha , Extratos de Tecidos/farmacologia , Alcanos , Animais , Anti-Inflamatórios , Relação Dose-Resposta a Droga , Ácidos Graxos Insaturados/análise , Camundongos , Fosfolipídeos/análise , Células RAW 264.7 , Extratos de Tecidos/química
17.
J Mater Sci Mater Med ; 19(12): 3473-9, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18592347

RESUMO

This study aims to develop a novel wound dressing comprising salmon milt DNA (sDNA) and salmon collagen (SC). The sDNA/SC composites were prepared by incubating a mixture of an acidic SC solution, an sDNA solution, and a collagen fibrillogenesis inducing buffer (pH 6.8) containing a crosslinking agent (water-soluble carbodiimide) for gelation, and a subsequent ventilation-drying process to give sDNA/SC films. The conjugation between sDNA and SC were confirmed by sDNA-elution assay and fluorescence microscopy. The sDNA/SC films with various doses of sDNA (sDNA/SC weight ratios of 1:5, 1:10, and 1:20) were used for in vitro cell cultures to evaluate their growth potentials of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK). It was found that NHDF proliferation was increased by sDNA conjugation, whereas NHEK proliferation was dose-dependently inhibited. In light of the in vitro results, the appropriate dose of sDNA for in vivo study was determined to be the ratio of 1:10. For the implantation in full-thickness skin defects in rat dorsal region, the sDNA/SC films were reinforced by incorporating them on a porous SC sponge, because the sDNA/SC films exhibited early contraction and inadequate morphologic stability when implanted in vivo. The regenerated tissue in the sDNA/SC sponge group showed similar morphology to native dermis, while the SC sponge group without sDNA showed epithelial overgrowth, indicating that additional sDNA could reduce epidermal overgrowth. Furthermore, blood capillary formation was significantly enhanced in the sDNA/SC sponge group when compared to the SC sponge group. In conclusion, the results suggest that the sDNA/SC composite could be a potential wound dressing for clinical applications.


Assuntos
Curativos Biológicos , Colágeno/química , DNA/química , Pele Artificial , Cicatrização , Animais , Tampões (Química) , Capilares/crescimento & desenvolvimento , Carbodi-Imidas/química , Proliferação de Células , Células Cultivadas , Reagentes para Ligações Cruzadas/química , Fibroblastos/citologia , Fibroblastos/transplante , Humanos , Concentração de Íons de Hidrogênio , Queratinócitos/citologia , Queratinócitos/transplante , Masculino , Peso Molecular , Fosfatos/química , Ratos , Ratos Wistar , Salmão , Pele/irrigação sanguínea , Pele/citologia , Pele/ultraestrutura , Solubilidade , Água/química , Cicatrização/fisiologia
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