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1.
Food Chem ; 305: 125454, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505413

RESUMO

The effects of the (-)-Epigallocatechin gallate (EGCG)-gelatin biofilm treatment (EGT) on microbial composition and quality of tilapia fillets stored at low temperatures were evaluated. The changes in mechanical properties, microbial reproduction, as well as lipid and protein oxidation during fillets storage were determined. The results showed that EGT reduced the microbial count and the relative abundance of the fillets. And EGT delayed the rate of lipid oxidation and protein denaturation in fillets. Compared with the control group, EGT samples had lower K values (74% on 18 d) and biogenic amines (39 mg/kg for putrescine and 50 mg/kg for cadaverine on 21 d). According to sensory evaluation, the shelf life of tilapia fillets was extended by 6 d in the EGT group. Therefore, EGT improved the quality of cryopreserved tilapia fillets and could be considered as a potential method for fish fillet preservation.

2.
J Oleo Sci ; 68(12): 1187-1197, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31735749

RESUMO

In this study, we first isolate phospholipid (PL) from Golden pompano head (GPH), and elucidate its structure. Gas chromatography (GC) was used to assess the GPH-PL fatty acid composition, Fourier transform infrared spectroscopy (FTIR) and ultraviolet absorption spectrometry (UV) were used for the qualitative analysis of GPH-PL, and LC-MS analysis was used to determine the major PL species. The results show that the contents of the various molecular species of GPH-PL were generally in the order phosphatidylcholine (PC) > sphingomyelin (SM) > lysophosphatidylcholine (LPC) > phosphatidylethanolamine (PE). The main molecular PC species are 16:0/18:2, 13:0/23:2, 27:2/9:0, 16:0/18:1, 12:0/22:2, 18:0/18:1, 18:0/24:1, and 18:1/24:0. The major SM species are 16:1/16:0, 16:0/18:1, 16:0/18:2, 16:0/26:2, and 18:1/24:1. The major LPC species are 18:1 and 16:0. The major PE species are 18:0/18:1 and 16:0/22:6. The total eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents in the GPH-PLs were 18.39%, and the content of DHA in the PL fraction was 16.47%. These results suggest that PLs from GPH is rich in polyunsaturated fatty acids (PUFA), which have good activity in anti-inflammation, anti-tumor, anti-osteoporosis and other aspects, and have important development prospects in the future.

3.
Food Chem Toxicol ; : 110886, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31626838

RESUMO

Diabetes mellitus has become a worldwide concern in recent years. In this study, the effect of Holothuria leucospilota polysaccharide (HLP) on type 2 diabetes mellitus (T2DM) was investigated in Goto-Kakizaki (GK) rats. The results showed that HLP significantly improved glucose intolerance and regulated blood lipid and hormone levels (p < 0.05). Pathological analysis showed that HLP repaired the impairments of the pancreas and colon in diabetic rats. In addition, a high dose of HLP (200 mg/kg) significantly upregulated the gene expression of peroxisome proliferator-activated receptor-α (PPAR-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), phosphoinositide 3-kinase (PI3K), protein kinase B (PKB/AKT), glucose transporter-4 (GLUT4) and anti-apoptotic (Bcl-2), and downregulated the mRNA levels of pro-apoptotic (Bax) and cluster of differentiation 36 (CD36) in diabetic rats (p < 0.05). Furthermore, HLP treatment increased the short-chain fatty acid-producing bacteria and decreased the opportunistic bacterial pathogen in the feces of diabetic rats. These results demonstrated that HLP has the potential to ameliorate T2DM in GK rats.

4.
Int J Mol Sci ; 20(19)2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31554265

RESUMO

Holothuria leucospilota polysaccharides (HLP) are expected to become potential resources for the treatment of hyperlipidemia because of their various bioactivities. In the study, the treatment of HLP on improving hyperlipidemia in rats was explored. Oral administration of HLP at 100 or 200 mg/kg body weight effectively alleviated serum lipid levels and liver histological abnormalities in high-fat-diet rats. HLP regulated abnormal mRNA, lipogenesis-related hormones and inflammatory cytokines (tumor necrosis factor-α, interleukin-6 and interleukin-12) levels. HLP improved the ability of gut microbiota to produce short-chain fatty acids (SCFAs). SCFAs have been found to ameliorate liver lesions. Therefore, HLP alleviated hyperlipidemia by improving the levels of SCFAs to regulate lipid metabolism. These results indicated that HLP could be used as beneficial polysaccharides to alleviate hyperlipidemia.

5.
Food Res Int ; 123: 217-225, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31284971

RESUMO

The impacts of the vacuum freeze (VFD), hot air (HAD), microwave (MD) and vacuum microwave (VMD) drying on the flavour of golden pompano fillets were evaluated using an electronic nose (E-nose), an electronic tongue (E-tongue) and simultaneous distillation extraction (SDE) - gas chromatography - mass spectrometry (GC-MS). The results showed that the E-nose and E-tongue systems could effectively differentiate volatile compounds of four samples. A total of 86 volatile flavour components were identified in the dried fillets; the main flavour components contained hydrocarbons (39), aldehydes (15), esters (10) and alcohols (9). HAD, MD and VMD processing promoted a gradual reduction in ketones and the generation of esters, while the fillets that were processed by VFD contained more hydrocarbon (29.68%) and alcohol (2.64%) compounds. The volatile compounds of dried golden pompano fillets were developed through four potential pathways, including the Maillard reaction, lipid oxidation and degradation, protein hydrolysis, and Strecker degradation.

6.
Food Chem ; 271: 204-210, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30236668

RESUMO

The effects of (-)-epigallocatechin gallate (EGCG) on the mechanical, antioxidative, antimicrobial, cross-linking and release properties of gelatin film were evaluated. The biofilm exhibited excellent antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli in the presence of EGCG at more than 3%. With the increasing content of EGCG (1%-5%), higher denaturation temperature (66.5-84.6 °C) and bloom strength (609.3-1114.7 g) and denser microstructure suggested the existence of cross-linking bonds in the biofilm. The infrared spectroscopy, protein pattern and release property of EGCG showed that hydrogen bonds were the main driving force for cross-linking of the biofilm. The release of EGCG from the biofilm system was described and two compounds were cross-linked via hydrogen bonds. Thus, this study confirmed the improvement on mechanical, bioactivities and release properties of the biofilm was most likely due to the interactions between gelatin and EGCG.


Assuntos
Catequina/química , Gelatina/química , Antioxidantes , Biofilmes
7.
J Oleo Sci ; 67(4): 407-417, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29526879

RESUMO

Human milk fat substitutes (HMFS) are rich in polyunsaturated fatty acids which upon microencapsulation, can be used as a source of high quality lipids in infant formula. The response surface methodology (RSM) was employed to optimize the microencapsulation condition of HMFS as a functional product. The microencapsulation efficiency (MEE) of microencapsulated HMFS was investigated with respect to four variables including concentration of soy lecithin (A), ratio of demineralized whey powder to malt dextrin (B), HFMS concentration (C), and homogenizing pressure (D). The optimum conditions for efficient microencapsulation of HMFS by the spray drying technique were determined as follows: the amount of soybean lecithin-0.96%, ratio of desalted whey powder to malt dextrin-2.04:1, oil content-17.37% and homogeneous pressure-0.46MPa. Under these conditions, the MEE was 84.72%, and the basic indices of the microcapsules were good. The structure of the microcapsules, as observed by scanning electron microscopy (SEM), revealed spherical, smooth-surfaced capsules with diameters ranging between 10-50 µm. Compared with HFMS, the peroxide value (POV) and acid value (AV) of the microcapsule were significantly lower during storage indicating that the microencapsulation process increases stability and shelf life. Infrared spectroscopic analyses indicated that HFMS had the same characteristic functional groups as the oil extracted from microcapsules. Simulated in vitro digestion revealed that the microcapsules were digested completely within 2h with maximum lipid absorption rate of 64%. Furthermore, these results advocate the embedding process of HFMS by RSM due to its efficacy.


Assuntos
Gorduras Insaturadas na Dieta , Composição de Medicamentos/métodos , Ácidos Graxos Insaturados , Fórmulas Infantis/química , Leite Humano/química , Cápsulas , Dextrinas , Armazenamento de Alimentos , Humanos , Lactente , Lecitinas , Microscopia Eletrônica de Varredura , Tamanho da Partícula , Peróxidos/análise , Pós , Soja , Espectrofotometria Infravermelho , Soro do Leite
8.
J Oleo Sci ; 64(12): 1273-81, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26582156

RESUMO

The present study attempts to investigate the anti-inflammatory potential of the isolated lipid extracts of three-spot seahorse which is rare marine bony fish. Petroleum ether (PE) extract was obtained from systematic solvent extraction after reflux extraction with 95% ethanol. FrIV was collected after silica gel column chromatography, and neutral lipids (NL), glycolipids (GL), phospholipids (PL) were separated from FrIV. Basic compositions were detected and analyzed via thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). Anti-inflammatory activities of total lipids (TL), isolated NL, GL, and PL were detected by secretion of pro-inflammatory cytokines induced by lipopolysaccharide (LPS) in murine monocyte macrophage RAW264.7 cells in vitro. The results revealed that lipids of seahorse showed a positive correlation with the in vitro suppression of the release of nitric oxide (NO), interleukin (IL)-6, IL-1ß and tumor necrosis factor (TNF)-α potently in a dose dependent manner, and showed cell compatibility. Among the fractions, GL (50 µg/mL) showed the highest capacity to attenuate the generation of pro-inflammatory cytokines which was comparable to that of the positive drug dexamethasone (DX) (20 µg/mL). Collectively, our findings indicated that the lipids from seahorse may be effective in the management of inflammation.


Assuntos
Anti-Inflamatórios/farmacologia , Citocinas/metabolismo , Mediadores da Inflamação/metabolismo , Lipídeos/isolamento & purificação , Lipídeos/farmacologia , Smegmamorpha/metabolismo , Animais , Dexametasona/farmacologia , Relação Dose-Resposta a Droga , Glicolipídeos/isolamento & purificação , Glicolipídeos/farmacologia , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Lipopolissacarídeos/farmacologia , Extração Líquido-Líquido/métodos , Camundongos , Óxido Nítrico/metabolismo , Fosfolipídeos/isolamento & purificação , Fosfolipídeos/farmacologia , Células RAW 264.7 , Extratos de Tecidos/isolamento & purificação , Extratos de Tecidos/farmacologia , Fator de Necrose Tumoral alfa/metabolismo
9.
J Oleo Sci ; 64(9): 933-42, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26250427

RESUMO

Three-Spot seahorse is a traditional medicine in Asian countries. However, the alcohol extract is largely unknown for its anti-inflammatory activity. This study aimed at elucidating fraction of potent anti-inflammatory activity of seahorse. A systematic solvent extraction method of liquid-liquid fractionation of ethanol crude extract gave four fractions petroleum ether (PE), and ethyl acetate (EtOAc), water saturated butanol (n-BuOH), water (H2O). In this study, PE extract was selected for further study after preliminary screening test, and was connected to silica column chromatography and eluted with different polarity of mobile phases, and obtained four active fractions (Fr I, Fr II, Fr III, Fr IV). Effect of separated fractions on inflammation was investigated in lipopolysaccharide (LPS) stimulated murine RAW264.7 cells in vitro. The result shows that seahorse extract was capable of inhibiting the production of nitric oxide (NO) significantly in a dose dependent manner and exhibited no notable cytotoxicity on cell viability. IC50 of fraction IV was 36.31 µg/mL, indicating that separated fraction possessed potent NO inhibitory activity against LPS-induced inflammatory response, thus, demonstrated its in vitro anti-inflammatory potentiality, it may be at least partially explained by the presence of anti-inflammation active substances, phenolic compounds, phospholipids and polyunsaturated fatty acids, especially phospholipids and polyunsaturated fatty acids. It could be suggested that seahorse lipid-soluble components could be used in functional food and anti-inflammatory drug preparations.


Assuntos
Citocinas/metabolismo , Mediadores da Inflamação/metabolismo , Lipopolissacarídeos/efeitos adversos , Macrófagos/metabolismo , Óxido Nítrico/metabolismo , Smegmamorpha , Extratos de Tecidos/farmacologia , Alcanos , Animais , Anti-Inflamatórios , Relação Dose-Resposta a Droga , Ácidos Graxos Insaturados/análise , Camundongos , Fosfolipídeos/análise , Células RAW 264.7 , Extratos de Tecidos/química
10.
J Mater Sci Mater Med ; 19(12): 3473-9, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18592347

RESUMO

This study aims to develop a novel wound dressing comprising salmon milt DNA (sDNA) and salmon collagen (SC). The sDNA/SC composites were prepared by incubating a mixture of an acidic SC solution, an sDNA solution, and a collagen fibrillogenesis inducing buffer (pH 6.8) containing a crosslinking agent (water-soluble carbodiimide) for gelation, and a subsequent ventilation-drying process to give sDNA/SC films. The conjugation between sDNA and SC were confirmed by sDNA-elution assay and fluorescence microscopy. The sDNA/SC films with various doses of sDNA (sDNA/SC weight ratios of 1:5, 1:10, and 1:20) were used for in vitro cell cultures to evaluate their growth potentials of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK). It was found that NHDF proliferation was increased by sDNA conjugation, whereas NHEK proliferation was dose-dependently inhibited. In light of the in vitro results, the appropriate dose of sDNA for in vivo study was determined to be the ratio of 1:10. For the implantation in full-thickness skin defects in rat dorsal region, the sDNA/SC films were reinforced by incorporating them on a porous SC sponge, because the sDNA/SC films exhibited early contraction and inadequate morphologic stability when implanted in vivo. The regenerated tissue in the sDNA/SC sponge group showed similar morphology to native dermis, while the SC sponge group without sDNA showed epithelial overgrowth, indicating that additional sDNA could reduce epidermal overgrowth. Furthermore, blood capillary formation was significantly enhanced in the sDNA/SC sponge group when compared to the SC sponge group. In conclusion, the results suggest that the sDNA/SC composite could be a potential wound dressing for clinical applications.


Assuntos
Curativos Biológicos , Colágeno/química , DNA/química , Pele Artificial , Cicatrização , Animais , Tampões (Química) , Capilares/crescimento & desenvolvimento , Carbodi-Imidas/química , Proliferação de Células , Células Cultivadas , Reagentes para Ligações Cruzadas/química , Fibroblastos/citologia , Fibroblastos/transplante , Humanos , Concentração de Íons de Hidrogênio , Queratinócitos/citologia , Queratinócitos/transplante , Masculino , Peso Molecular , Fosfatos/química , Ratos , Ratos Wistar , Salmão , Pele/irrigação sanguínea , Pele/citologia , Pele/ultraestrutura , Solubilidade , Água/química , Cicatrização/fisiologia
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