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1.
J Am Chem Soc ; 2023 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-36730431

RESUMO

Ferroptosis, a newly discovered form of regulated cell death, is emerging as a promising approach to tumor therapy. However, the spatiotemporal control of cell-intrinsic Fenton chemistry to modulate tumor ferroptosis remains challenging. Here, we report an oxazine-based activatable molecular assembly (PTO-Biotin Nps), which is capable of triggering the lysosomal dysfunction-mediated Fenton pathway with excellent spatiotemporal resolution via near-infrared (NIR) light to evoke ferroptosis. In this system, a pH-responsive NIR photothermal oxazine molecule was designed and functionalized with a tumor-targeting hydrophilic biotin-poly(ethylene glycol) (PEG) chain to engineer well-defined nanostructured assemblies within a single-molecular framework. PTO-Biotin Nps possesses a selective tropism to lysosome accumulation inside tumor cells, accommodated by its enhanced photothermal activity in the acidic microenvironment. Upon NIR light activation, PTO-Biotin Nps promoted lysosomal dysfunction and induced cytosolic acidification and impaired autophagy. More importantly, photoactivation-mediated lysosomal dysfunction via PTO-Biotin Nps was found to markedly enhance cellular Fenton reactions and evoke ferroptosis, thereby improving antitumor efficacy and mitigating systemic side effects. Overall, our study demonstrates that the molecular engineering approach of pH-responsive photothermal oxazine assemblies enables the spatiotemporal modulation of the intrinsic ferroptosis mechanism, offering a novel strategy for the development of metal-free Fenton inducers in antitumor therapy.

2.
BMC Geriatr ; 23(1): 66, 2023 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-36732687

RESUMO

BACKGROUND: For elderly adults undergoing hip arthroplasty, fascia iliaca compartment block (FICB) is often used before spinal anesthesia to reduce the pain of posture placement. However, the impact of FICB within 48 h needs further study. METHODS: 89 elderly adults scheduled to undergo arthroplasty for hip fracture were enrolled and randomized into the FICB group (n = 45) and the control group (n = 44). The fascia iliaca on the operated side was located using ultrasound, and a puncture needle was placed under the fascia iliaca. The FICB group was injected with 40 ml of 0.5% ropivacaine, and the control group was injected with 40 ml of normal saline. Spinal anesthesia was performed after 20 min. Our primary outcome measures were: duration of analgesia, muscle strength, and Quality of Recovery (QoR). RESULTS: The duration of analgesia in the FICB group was 403.5 ± 39.6 min, which was longer than that (357.5 ± 35.9 min) of the control group (P = 0.012). There were 19 (42.2%) patients with muscle strength of grade 4 in the FICB group and 36 (81.8%) patients with muscle strength of grade 4 in the control group. FICB group was lower (P < 0.001). QoR-15 at 24 h after surgery was 114.1 ± 8.3 in the FICB group and 104.6 ± 8.4 in the control group (P < 0.001). QoR-15 at 48 h after surgery was 122.7 ± 8.4 in the FICB group and 120.5 ± 9.5 in the control group (P = 0.232). CONCLUSIONS: For elderly adults with hip fractures, FICB provided longer analgesia and improved 24-h QoR, but reduced postoperative muscle strength. TRAIL REGISTRATION: Chinese Clinical Registry Center, ChiCTR2200056937, 23/02/2022.

3.
Parkinsonism Relat Disord ; 108: 105296, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36682278

RESUMO

BACKGROUND: Progressive supranuclear palsy (PSP) is a rare, disabling, neurodegenerative disease, with few studies done in Asian populations. METHODS: We prospectively characterized the clinical features and disease burden in a consecutively-recruited multi-ethnic Asian PSP cohort. Patients were extensively phenotyped using the Movement Disorder Society (MDS-PSP) clinical diagnostic criteria and the PSP-Clinical Deficits Scale (PSP-CDS). Caregiver burden was measured using the modified Zarit Burden Interview (ZBI). Investigations (neuroimaging and genetic tests) were reviewed. RESULTS: There were 104 patients (64.4% male; 67.3% Chinese, 21.2% Indians, 9.6% Malays), consisting of 48.1% Richardson syndrome (PSP-RS), 37.5% parkinsonian phenotype (PSP-P), and 10.6% progressive gait freezing phenotype (PSP-PGF). Mean age at motor onset was 66.3 ± 7.7 years, with no significant differences between the PSP phenotypes. Interestingly, REM-sleep behaviour disorder (RBD) symptoms and visual hallucinations (considered rare in PSP) were reported in 23.5% and 22.8% of patients, respectively, and a family history of possible neurodegenerative or movement disorder in 20.4%. PSP-CDS scores were highest (worst) in PSP-RS; and correlated moderately with disease duration (rs = 0.45, P < 0.001) and weakly with caregiver burden (rs = 0.22, P = 0.029) in the overall cohort. Three of 48 (6.3%) patients who had whole-exome sequencing harboured pathogenic/likely pathogenic GBA variants. CONCLUSIONS: Significant heterogeneity in clinical features and disease burden, and high rates of RBD symptoms, visual hallucinations, and familial involvement were observed in this relatively large cohort. Our findings highlight important considerations when assessing Asian patients, and provide further support for the notion of overlapping neurobiology between PSP and Lewy body disorders.

4.
BMC Ophthalmol ; 23(1): 36, 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36703165

RESUMO

BACKGROUND: To compare the characteristics of corneal thickness measurements among the RTVue, Casia-2, and Pentacam in patients with mild-to-moderate keratoconus. METHODS: We recruited 46 eyes of 46 patients diagnosed with mild-to-moderate keratoconus at our hospital between January and March 2022. The central corneal thickness (CCT) and thinnest corneal thickness (TCT) were measured using two optical coherence tomography (OCT) instruments (RTVue and Casia-2) and the more conventional Pentacam. Differences and correlations between the CCTs and TCTs, based on the device and influencing factors, were explored. RESULTS: The CCTs were highly consistent among the groups (p = 0.434) and correlated with one another (p < 0.001). The TCTs measured by OCTs were thinner than those measured by the Pentacam (p < 0.001); however, all three devices were highly correlated (p < 0.001). The thinnest point location measurements with RTVue and Casia-2 differed significantly from the measurements with the Pentacam. Bland-Altman plots demonstrated a significant agreement between Pentacam and OCTs in TCT measurement (p < 0.001); the 95% limits of agreement were - 3.1 µm to + 33.1 µm for Pentacam and RTVue and - 8.6 µm to + 36.5 µm for Pentacam and Casia-2. RTVue and Casia-2 showed no difference in corneal thickness (p = 0.633) and thinnest point location measurement (p > 0.05). Multivariate analysis identified that the TCT measurement difference between the RTVue and Pentacam was related to the difference between the CCT and TCT (b = 0.490, 95% confidence interval [CI]: 0.033 to 0.948, p = 0.036), whereas the difference between the Casia-2 and Pentacam was related to the anterior radius for curvature (A) grade (b = 3.9, 95% CI: 1.753 to 6.074, p = 0.001), corneal pachymetry at the thinnest (C) grade (b = - 7.875, 95% CI: - 11.404 to - 4.346, p < 0.001), and the difference between the CCT and TCT (b = 0.425, 95% CI: 0.1 to 0.751, p = 0.012). CONCLUSIONS: CCTs in patients with mild-to-moderate keratoconus were similar among all three devices, but the TCTs and the thinnest point locations were not. Furthermore, the TCT measurement differences between the OCT devices and the Pentacam were more pronounced in keratoconus cases with a steeper anterior surface, thicker TCTs, and a larger difference between the CCT and TCT. TRIAL REGISTRATION: Number: 2021118-1. Retrospectively registered: September 01, 2021.


Assuntos
Ceratocone , Humanos , Paquimetria Corneana/métodos , Ceratocone/diagnóstico , Córnea , Estudos Prospectivos , Reprodutibilidade dos Testes , Topografia da Córnea/métodos , Tomografia de Coerência Óptica/métodos
5.
Ultrason Sonochem ; 93: 106307, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36706668

RESUMO

In our previous paper, we derived a new single bubble model including the effect of bulk viscosity. To confront it to experiments, single bubble dynamics was measured here in 30% (v/v) glycerol-water mixture under different acoustic amplitudes and compared to models including or not the effect of bulk viscosity. The results showed that calculated bubble dynamics were not significantly affected by the bulk viscosity within the experimental conditions used in this study. However, there was a noticeable delay for the first rebound when bulk viscosity was considered. The corresponding sonoluminescence intensities were collected and compared with theoretical predictions. The results did not allow to discriminate between the two models (one includes the effect of bulk viscosity, the other does not), confirming the negligible effect of bulk viscosity in this condition (30% (v/v) glycerol-water mixture). Due to the instability of a single bubble in higher viscosity solutions, we could not implement experiments that can discriminate between the two models.

6.
Nanoscale ; 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36662239

RESUMO

The operational stability of reduced-dimensional metal halide perovskites (RD-MHPs) lags far behind the practical requirements for future high-definition displays. Thereinto, the electron durability of RD-MHPs plays a critical role in stable LEDs during continuous operation, however, it still lacks adequate research and a deep understanding. Herein, the electron durability and deterioration mechanism of phenethylammonium (PEA+)-modified RD-MHPs are systematically conducted through an in situ photoelectron spectroscopy technique by implementing tunable electron-beam radiation to simulate device operation. The formation of detrimental metallic lead (Pb0) caused by the reduction of lead ions (Pb2+) is observed along with the decomposition of PEA+ under electron-beam radiation, thereby changing the photophysical properties of PEA+-doped RD-MHPs. These results provide deep insight into the process of how injected electrons affect the performance of PEA+-doped perovskite LEDs, which may also provide potential guidance for designing robust and effective organic spacers for RD-MHPs.

7.
Adv Mater ; : e2209958, 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36693075

RESUMO

High-dielectric-constants polymer composites have broad application prospects in flexible electronics and electrostatic energy storage capacitors. Substantial enhancement in dielectric constants (εr ) of polymer composites so far can only be obtained at high loading of nanofillers, resulting in high dielectric loss and high elastic modulus of polymer composites. Addressing the polarization shielding and the consequent polarization discontinuity at polymer/filler interfaces has been a long-standing challenge to achieve flexible polymer composite with high εr . Herein, we propose and demonstrate a polymer composite with interconnected BaTiO3 (BT) ceramic scaffold exhibiting a high εr ∼ 210 at low BT volume fraction of ∼ 18 vol.%, approaching the upper limit predicted by the parallel model. By incorporating relaxor Ba(Zrx Ti1-x )O3 phase in BT scaffold, we further achieved excellent dielectric temperature stability with Δεr below ±10% within broad temperature range (25-140 °C). Moreover, the dielectric performances remain stable under compressive strain up to 80%. This work provides a facile approach to construct large-scale polymer composites with robust dielectric performance against changes in thermal and mechanical condition, which are promising for high-temperature applications in flexible electronics. This article is protected by copyright. All rights reserved.

8.
Biomark Res ; 11(1): 8, 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36691065

RESUMO

BACKGROUND: Gilteritinib is the only drug approved as monotherapy for acute myeloid leukemia (AML) patients harboring FMS-like tyrosine kinase 3 internal tandem duplication (FLT3-ITD) mutation throughout the world. However, drug resistance inevitably develops in clinical. Sitravatinib is a multi-kinase inhibitor under evaluation in clinical trials of various solid tumors. In this study, we explored the antitumor activity of sitravatinib against FLT3-ITD and clinically-relevant drug resistance in FLT3 mutant AML. METHODS: Growth inhibitory assays were performed in AML cell lines and BaF3 cells expressing various FLT3 mutants to evaluate the antitumor activity of sitravatinib in vitro. Immunoblotting was used to examine the activity of FLT3 and its downstream pathways. Molecular docking was performed to predict the binding sites of FLT3 to sitravatinib. The survival benefit of sitravatinib in vivo was assessed in MOLM13 xenograft mouse models and mouse models of transformed BaF3 cells harboring different FLT3 mutants. Primary patient samples and a patient-derived xenograft (PDX) model were also used to determine the efficacy of sitravatinib. RESULTS: Sitravatinib inhibited cell proliferation, induced cell cycle arrest and apoptosis in FLT3-ITD AML cell lines. In vivo studies showed that sitravatinib exhibited a better therapeutic effect than gilteritinib in MOLM13 xenograft model and BaF3-FLT3-ITD model. Unlike gilteritinib, the predicted binding sites of sitravatinib to FLT3 did not include F691 residue. Sitravatinib displayed a potent inhibitory effect on FLT3-ITD-F691L mutation which conferred resistance to gilteritinib and all other FLT3 inhibitors available, both in vitro and in vivo. Compared with gilteritinib, sitravatinib retained effective activity against FLT3 mutation in the presence of cytokines through the more potent and steady inhibition of p-ERK and p-AKT. Furthermore, patient blasts harboring FLT3-ITD were more sensitive to sitravatinib than to gilteritinib in vitro and in the PDX model. CONCLUSIONS: Our study reveals the potential therapeutic role of sitravatinib in FLT3 mutant AML and provides an alternative inhibitor for the treatment of AML patients who are resistant to current FLT3 inhibitors.

9.
J Control Release ; 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36708880

RESUMO

Hepatocellular carcinoma (HCC) remains one of the leading causes of cancer-related deaths worldwide, however, current clinical diagnostic and treatment approaches remain relatively limited, creating an urgent need for the development of effective technologies. Immunotherapy has emerged as a powerful treatment strategy for advanced cancer. The number of clinically approved drugs for HCC immunotherapy has been increasing. However, it remains challenging to improve their transport and therapeutic efficiency, control their targeting and release, and mitigate their adverse effects. Nanotechnology has recently gained attention for improving the effectiveness of precision therapy for HCC. We summarize the key features of HCC associated with nanoparticle (NPs) targeting, release, and uptake, the roles and limitations of several major immunotherapies in HCC, the use of NPs in immunotherapy, the properties of NPs that influence their design and application, and current clinical trials of NPs in HCC, with the aim of informing the design of delivery platforms that have the potential to improve the safety and efficacy of HCC immunotherapy,and thus, ultimately improve the prognosis of HCC patients.

11.
Artigo em Inglês | MEDLINE | ID: mdl-36625987

RESUMO

INTRODUCTION: Drug-induced QT interval prolongation has been reported to be related to life-threatening polymorphic ventricular tachycardia (torsade de pointes). Proton pump inhibitors (PPIs) are prescribed widely for hospitalized patients; the QT interval prolongation and torsade de pointes caused by PPIs were reported. We conducted a study to determine the association between PPI treatment and QT interval prolongation in critically ill patients. METHODS: This study included patients with electrocardiography (ECG) reports from the Medical Information Mart for Intensive Care III database (MIMIC-III). Patients younger than 18 years, missing baseline laboratories and with QT interval prolongation before intensive care unit (ICU) admission were excluded. The end point was the diagnosis of QT interval prolongation reported by ECG. RESULTS: This study included 24,512 ICU patients. Of them, 11,327 patients were treated with PPIs, 4181 with histamine 2 receptor antagonists (H2RAs) and 6351 without acid suppression therapy (non-AST); the incidence of QT interval prolongation were 8.5%, 3.3% and 3.4% respectively. After adjustment for demographics, electrolytes, comorbidities and medications, PPIs were associated a higher risk of QT interval prolongation compared with H2RAs (OR 1.66, 95% CI 1.36 - 2.03) and non-AST (OR 1.54, 95% CI 1.31 - 1.82), while there was not significant difference between H2RAs and non-AST (OR 0.93, 95% CI 0.73 - 1.17). In the propensity score matching population, the results were consistent. Pantoprazole (OR 2.14, 95% CI 1.52 - 3.03) and lansoprazole (OR 1.80, 95% CI: 1.18 - 2.76) showed a higher QT prolongation risk than omeprazole. Several drugs caused higher QT prolongation risk when used in combination with PPIs. CONCLUSION: In ICU patients, the association between PPI prescription and increased risk of QT interval prolongation was independent of known QT-prolonging factors; pantoprazole and lansoprazole had a higher risk compared with omeprazole. The combination of PPIs and other QT-prolonging drugs should be avoided.

12.
BMC Pediatr ; 23(1): 13, 2023 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624390

RESUMO

BACKGROUND: Type 2 innate lymphoid cells (ILC2) are upregulated in childhood allergic rhinitis (AR) and are associated with AR severity. This study aimed to investigate changes in the ILC2 milieu in pediatric patients with AR after sublingual immunotherapy (SLIT). METHODS: Forty- pediatric patients with AR received house dust mite (HDM) allergen extract for SLIT group and thirty pediatric patients received placebo in the study, respectively. The levels of ILC2, ILC2-related cytokines (IL-5/IL-13) and their transcription factors (GATA binding protein 3, retinoic acid-related orphan receptor α) in the circulation were assessed after 1- and 2-year SLIT. Moreover, peripheral blood mononuclear cells (PBMCs) in patients were prepared and stimulated by recombinant thymic stromal lymphopoietin, IL-25, and IL-33 after 2-year SLIT. Subsequently, the levels of ILC2, IL-5, and IL-13 were tested. RESULTS: The frequency of ILC2 and the levels of their transcription factors in the circulation were significantly decreased after SLIT in the SLIT group. The levels of ILC2-related cytokines in the SLIT group showed the same trend. The frequency of ILC2 was positively correlated with transcription factors and cytokines after SLIT. SLIT was observed to reduce the ability of HDM sensitization to generate the ILC2 milieu in PBMCs. CONCLUSIONS: Changes in the ILC2 milieu may be correlated with the curative effect and immune regulation function of SLIT. Our results suggested that the regulatory effect on ILC2 is part of the therapeutic mechanism of SLIT.


Assuntos
Rinite Alérgica , Imunoterapia Sublingual , Criança , Humanos , Alérgenos , Citocinas , Imunidade Inata , Fatores Imunológicos , Interleucina-13 , Interleucina-5 , Leucócitos Mononucleares , Linfócitos/metabolismo , Rinite Alérgica/terapia , Imunoterapia Sublingual/métodos , Fatores de Transcrição , Resultado do Tratamento
13.
Sci Total Environ ; 864: 161173, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36572315

RESUMO

The extensive use of antibiotics leading to the rapid spread of antibiotic resistance poses high health risks to humans, but to date there is still lack of an on-site detection method of SA residues. In this study, we integrated radical polymerization using sodium p-styrenesulfonate as a functional monomer and the self-polymerization of dopamine to prepare double-system imprinted polymers (DIPs) using sulfonamide antibiotics as templates. We found that the DIPs were semi-interpenetrating polymer networks and introduction of poly(dopamine) improved the selectivity of the imprinted cavities as well as the conductivity. The selectivity and sensitivity of the sensor using DIPs were much higher than those using single-system MIPs. This sensor could determine sulfonamides in complex samples in the presence of structural analogues. The linear range was from 0.01 to 10.00 µmol L-1 with a detection limit of 4.00 nmol L-1. Furthermore, based on the highly selective DIPs and statistics analysis, this method could be used for simultaneous analysis of 4 sulfonamide types in real samples with an accuracy of 94.87 %. This work provides a strategy to improve the selectivity and sensitivity of MIPs based-sensor that can serve as tool for the simultaneous analysis of antibiotic residues in environment samples.


Assuntos
Impressão Molecular , Humanos , Impressão Molecular/métodos , Sulfonamidas , Polímeros/química , Técnicas Eletroquímicas/métodos , Dopamina , Sulfanilamida , Antibacterianos
14.
Org Lett ; 25(1): 240-245, 2023 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-36573686

RESUMO

Herein, we have developed an efficient tungsten-catalyzed homogeneous system for oxidative dehydrogenative coupling of anilines to selectively produce various azoaromatics and azoxyaromatics as well as 2-substituted indolone N-oxides by simply regulating the reaction solvent with peroxide as a terminal oxidant under additive-free conditions. These findings provide an experimental framework for exploring tungsten catalysis in organic synthesis and offer an efficient and convenient tactic for the selective oxidation of anilines.


Assuntos
Compostos de Anilina , Tungstênio , Oxirredução , Catálise , Estresse Oxidativo
15.
Sci Total Environ ; 865: 161212, 2023 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-36586687

RESUMO

Isoprene is the most abundantly emitted biogenic volatile organic compound (BVOC), which plays an essential role in producing tropospheric ozone (O3). However, the simulations of isoprene emissions have not been sufficiently verified over Yangtze River Delta (YRD), and few studies have specifically addressed its impact on O3 formation. In this study, we simulated the isoprene emissions in Zhejiang Province (ZJ), a region with the largest BVOC emission in YRD, in August 2020 using the Model of Emissions of Gases and Aerosols from Nature (MEGAN) and the latest Moderate Resolution Imaging Spectroradiometer (MODIS) products, and investigated its contributions to O3 using the Weather Research and Forecasting (WRF)-Community Multiscale Air Quality (CMAQ) model. The model has a good performance on isoprene simulations over urban and suburban areas, with mean biases of -0.16-0.12 ppb, but underestimated the concentrations at forest sites (mainly due to bamboo). Regionally, the simulated formaldehyde concentrations over forests agree well with the satellite observations. In August 2020, the total isoprene emission in ZJ was 125.1 GgC, with higher emissions in western ZJ and relatively lower emissions in eastern coastal regions. The spatial pattern of isoprene concentrations is similar to its emissions, and the maximum daytime average concentrations are above 3.5 ppb. The spatial pattern of its contribution to daily maximum 8 h average O3 concentrations is significantly different from the emissions and concentrations, which shows a higher impact in northern ZJ (>6 ppb) and relatively lower impact in southern ZJ (1-3 ppb). The mean contribution over ZJ is 8.9 %, with daily variation in the range of 3.1 % to 13.4 %. For different cities, the monthly mean contribution is in the range of 4.6 % to 14.3 %, and the maximum daily contribution reaches about 25 %. These findings help understand the summertime O3 pollution in ZJ and the YRD region of China.

16.
J Mov Disord ; 16(1): 91-94, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36537064

RESUMO

KMT2B-linked dystonia (DYT-KMT2B) is a childhood-onset dystonia syndrome typically beginning in the lower limbs and progressing caudocranially to affect the upper limbs with eventual prominent craniocervical involvement. Despite its recent recognition, it now appears to be one of the more common monogenic causes of dystonia syndromes. Here, we present an atypical case of DYT-KMT2B with oromandibular dystonia as the presenting feature, which remained restricted to this region three decades after symptom onset. This appears to be the first reported case of DYT-KMT2B from Southeast Asia and provides further supporting evidence for the pathogenic impact of the KMT2B c.6210_6213delTGAG variant.

17.
Fish Shellfish Immunol ; 132: 108484, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36516955

RESUMO

Tumor necrosis factor ligand superfamily member 6 (TNFSF6), also known as FasL/CD95L, is essential for maintaining the body's immune homeostasis. However, the current reports on TNFSF6 in fish are relatively scarce. In the present study, we conducted functional analyses of a TNFSF6 (TroTNFSF6) from the teleost fish golden pompano (Trachinotus ovatus). TroTNFSF6 is composed of 228 amino acids and has a low similarity with other species (9.65%-58.79%). TroTNFSF6 was expressed in the 11 tissues tested and was significantly up-regulated after Edwardsiella tarda infection. In vivo, overexpression of TroTNFSF6 effectively stimulated the AKP and ACP activities, and reduced bacterial infection in fish tissues. Correspondingly, knockdown of TroTNFSF6 expression resulted in increasing bacterial dissemination and colonization in fish tissues. In vitro, recombinant TroTNFSF6 protein promoted the proliferation of T. ovatus head kidney lymphocytes (HKLs), and promoted the apoptosis of murine liver cancer cells (Hepa1-6). The results indicated that TroTNFSF6 plays an important role in the T. ovatus antibacterial immunity. These observations will facilitate the future in-depth study of teleost TNFSF6.


Assuntos
Doenças dos Peixes , Perciformes , Doenças dos Roedores , Animais , Camundongos , Imunidade Inata/genética , Proteínas de Peixes , Ligantes , Peixes , Proteínas Recombinantes , Fator de Necrose Tumoral alfa
18.
Phys Chem Chem Phys ; 25(3): 2090-2097, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36562283

RESUMO

Here, a "chemical unit co-substitution" method is used to improve the near-infrared (NIR) emission of phosphors, using [Zn2+-Ge4+] to co-substitute [Ga3+-Ga3+] sites to reduce crystal field splitting to affect the structure of gallium oxide. A series of broadband NIR phosphors are synthesized by a high-temperature solid-phase method, and their phase structures, crystal structures, morphologies, diffuse reflectance spectra, and luminescence lifetimes are investigated. The Ga1.68(Zn-Ge)0.3O3:0.02Cr3+ (GZGOC) phosphor exhibits NIR wide-band emission, with a peak wavelength of 766 nm and a half-width of 138 nm. Meanwhile, the quantum yield of photoluminescence can reach 81.2%. The phosphor has good thermal stability. When the temperature reaches 373 K, its emission intensity still remains at 73.4% of that at room temperature. A 460 nm LED chip and this phosphor are used to fabricate a phosphor-converted light emitting diode (pc-LED) device which can be used as a NIR light source. All these results show the application potential of the as-prepared phosphor in NIR imaging.

19.
Biomacromolecules ; 24(1): 471-480, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36548941

RESUMO

Rationally designing microstructures of soft hydrogels for specific biological functionalization is a challenge in tissue engineering applications. A novel and affordable soft hydrogel scaffold is constructed here by incorporating polyphenol modules with lysozyme amyloid fibrils (Lys AFs) via non-covalent self-assembly. Embedded polyphenols not only trigger hydrogel formation but also determine gel behavior by regulating the polyphenol gallol density and complex ratio. The feasibility of using a polyphenol-Lys AF hydrogel as a biocompatible cell scaffold, which is conducive to cell proliferation and spreading, is also shown. Notably, introducing polyphenols imparts the corresponding hydrogels a superior cell bioadhesive efficiency without further biofunctional decoration and thus may be successfully employed in both healthy and cancer cell lines. Confocal laser scanning microscopy also reveals that the highly expressed integrin-mediated focal adhesions form due to stimulation of the polyphenol-AF composite hydrogel, direct cell adhesion, proliferation, and spreading. Overall, this work constitutes a significant step forward in creating highly adhesive tissue culture platforms for in vitro culture of different cell types and may greatly expand prospects for future biomaterial design and development.


Assuntos
Adesivos , Hidrogéis , Hidrogéis/farmacologia , Hidrogéis/química , Polifenóis/farmacologia , Polifenóis/química , Materiais Biocompatíveis/farmacologia , Engenharia Tecidual , Amiloide/química , Proteínas Amiloidogênicas
20.
Rev. bras. med. esporte ; 29: e2022_0157, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1394817

RESUMO

ABSTRACT Introduction: The increase in the number of students experiencing depression severely impacts the physical and mental health of college students. Objective: Explore the effect of an exercise intervention on patients with depression by analyzing its rehabilitation effect. Methods: Physical education combined with extracurricular activities were chosen. The experimental physical education group completed about 45 minutes of low-intensity running, performing physical recovery and rest under teacher guidance. The sport form of extracurricular activity was basketball, twice a week. Physical education combined with extracurricular activities totaled a total of 4 times a week, lasting six weeks. Results: The exercise intervention reduced the participants' psychological test scores, relieving them of their psychological conditions. The exercise intervention improved somatization factors (obsessive-compulsive, depression, interpersonal, paranoid, anxiety, and negative symptoms). Conclusion: The personalized exercise plan is recommended for students with depression, with the promotion of corresponding physical exercise out of class, aiming to improve students' psychological state and mental health. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução: O aumento no número de estudantes que sofrem depressão tem um impacto negativo severo na saúde física e mental dos estudantes universitários. Objetivo: Explorar o efeito da intervenção do exercício em pacientes com depressão, analisando o seu efeito de reabilitação. Métodos: Elegeu-se a forma de educação física combinada com atividades extracurriculares. O grupo experimental de educação física completou cerca de 45 minutos de corrida de baixa intensidade, realizando a recuperação física e o descanso sob orientação dos professores. A forma esportiva de atividades extracurriculares foi o basquete, duas vezes por semana. A educação física combinada com as atividades extracurriculares totalizou um total de 4 vezes semanais, com duração total de seis semanas. Resultados: A intervenção no exercício teve efeito redutor nos escores do teste psicológico dos participantes, revelando um alívio de suas condições psicológicas. A intervenção no exercício apresentou efeitos evidentes sobre a melhoria dos fatores de somatização (sintomas obsessivo-compulsivos, depressão, interpessoais, paranoicos, de ansiedade e hostis). Conclusão: O plano de exercício personalizado é recomendado para os alunos com depressão, sendo a promoção do exercício físico correspondente extraclasse, visando melhorar o estado psicológico dos alunos e melhorar sua saúde mental. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción: El aumento del número de estudiantes que sufren depresión tiene un grave impacto negativo en la salud física y mental de los universitarios. Objetivo: Explorar el efecto de la intervención de ejercicio en pacientes con depresión analizando su efecto de rehabilitación. Métodos: Se eligió la forma de educación física combinada con actividades extraescolares. El grupo experimental de educación física realizó unos 45 minutos de carrera de baja intensidad, realizando recuperación física y descanso bajo la dirección de los profesores. La forma deportiva de las actividades extracurriculares era el baloncesto, dos veces por semana. La educación física combinada con las actividades extraescolares se realizaba un total de 4 veces por semana, con una duración total de seis semanas. Resultados: La intervención de ejercicio tuvo un efecto reductor en las puntuaciones de las pruebas psicológicas de los participantes, revelando un alivio de sus condiciones psicológicas. La intervención del ejercicio mostró efectos evidentes en la mejora de los factores de somatización (síntomas obsesivo-compulsivos, de depresión, interpersonales, paranoides, de ansiedad y hostiles). Conclusión: Se recomienda el plan de ejercicio personalizado para los alumnos con depresión, con la promoción del correspondiente ejercicio físico extra clase dirigido a mejorar el estado psicológico de los alumnos y a mejorar su salud mental. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

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