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1.
Pharmacol Res ; 152: 104606, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31846760

RESUMO

Proton pump inhibitors (PPIs) are used widely for the treatment of acid-related disorders. Despite their excellent efficacy and tolerance, the pharmacodynamics and pharmacokinetics of PPIs are affected by each patient's CYP2C19 and gastric H+,K+-ATPase genotype. The aim of this review was to analyze the effect of genetic polymorphisms on the pharmacodynamic and pharmacokinetic properties of PPIs. The gastric acid-suppressive effect of PPIs is affected by both gastric H+,K+-ATPase and CYP2C19 polymorphisms, although gastric H+,K+-ATPase polymorphisms may have larger effects. Ilaprazole and rabeprazole show relatively small differences in the pharmacokinetic and pharmacodynamic properties and clinical efficacy among the different CYP2C19 genotypes. Compared with oral administration, the intravenous infusion of PPIs is less affected by CYP2C19 polymorphism. At the same dose, each enantiomer has less variation among different CYP2C19 genotypes than a racemate mixture.

2.
Environ Sci Pollut Res Int ; 26(30): 30826-30835, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31444729

RESUMO

The sequential extraction method was used to determine the fraction of arsenic (As) in different-sized particulate matters (PMs) (i.e., PM2.5, PM10, and total suspended particles (TSP)). Samples were collected from Baoding, a typical medium-sized city with the serious haze pollution in China. The bioavailabilities of As in the samples were estimated based on the fraction results. A large percentage of fine particles were detected in TSP, with the average PM2.5/PM10 and PM10/TSP ratios all above 0.69. The total concentrations of As in PM2.5, PM10, and TSP samples were in the range of 4.5-296.4, 14.1-708.0, and 32.8-798.0 ng m-3, respectively. The mass percentages of As in PM2.5, PM2.5-10, and PM10-100 were calculated; the results indicated that As tended to concentrate in fine particles. PM-bound As mainly presented in the nonspecifically sorbed fraction (F1) during all of the sampling periods. The percentages of F1-As and bioavailability of As were higher in PM2.5, followed by PM10 and TSP. By contrast, the residual fraction (F5-As) contents declined in the order of TSP > PM10 > PM2.5. Significant differences in the speciation and bioavailability of As in different-sized PMs were found, and the influence of particle size on the speciation and bioavailability of As in PMs was verified. Fine particles adsorbed more As with higher bioavailability, and potentially led to more serious adverse effects on human health than the larger ones.


Assuntos
Poluentes Atmosféricos/análise , Arsênico/análise , Material Particulado/análise , Poluentes Atmosféricos/farmacocinética , Arsênico/farmacocinética , Disponibilidade Biológica , China , Cidades , Monitoramento Ambiental , Humanos , Tamanho da Partícula , Material Particulado/química
3.
Environ Monit Assess ; 191(8): 528, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31367959

RESUMO

The distribution and bioavailability of arsenic (As) in indoor/outdoor total suspended particulates (TSP), inhalable particulate matters (PM10), and fine particulate matters (PM2.5) in Baoding, China were investigated. The average I/O ratios for TSP, PM10, and PM2.5 were 0.52, 0.66, and 0.96, respectively. There was no significant correlation between indoor/outdoor TSP, PM10, and PM2.5. The indoor/outdoor concentrations of As surpassed the limited value of As. I/O ratios of arsenic in TSP, PM10, and PM2.5 were 0.52, 0.58, and 0.55, respectively. The contents of arsenic in different fractions were mainly affected by the total concentrations of arsenic in particulate matters (PM) rather than the particle sizes for TSP and PM10. Arsenic was mainly in non-specifically sorbed fraction (F1) in both indoor and outdoor PM2.5. The evaluated carcinogenic risk (CR) was within the safe level. The bioavailability of As increased with particle size decreasing for both indoor and outdoor PM. The potential bioavailability of As in outdoor particles was higher than that of indoor particles with the same size, especially PM2.5.


Assuntos
Poluentes Atmosféricos/análise , Arsênico/análise , Material Particulado/química , Poluentes Atmosféricos/química , Poluição do Ar em Ambientes Fechados/análise , Arsênico/química , China , Monitoramento Ambiental , Humanos , Tamanho da Partícula , Material Particulado/análise
4.
Int J Clin Pharmacol Ther ; 57(11): 552-560, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31397275

RESUMO

OBJECTIVE: This study was conducted to evaluate the difference in acid inhibition function among lansoprazole (LPZ), pantoprazole (PPZ), and their respective stereoisomers following single and multiple intravenous doses in healthy Chinese subjects. MATERIALS AND METHODS: The dosage groups were set as follows: 30 mg single and multiple intravenous administrations of LPZ or R-LPZ, 40 mg single and multiple intravenous administrations of PPZ or S-PPZ. Subjects received an intravenous infusion of LPZ, R-LPZ, PPZ, or S-PPZ injection in sterile saline solution (100 mL/h, 60 minutes), respectively. The intragastric pH was sampled every second for 24 hours at baseline and for 24 hours after drug administration. The baseline-adjusted pharmacodynamic (PD) parameters include ΔMean (pH), ΔMedian (pH), ΔTpH≥3 (%), ΔTpH≥4 (%), ΔTpH≥6 (%), and ΔAUECph-tτ1-τ2. The PD parameters were evaluated in different time intervals (0 - 24 hours, 0 - 4 hours and 14 - 24 hours). RESULTS: After a single dose, the ΔTpH≥4 (%) of R-LPZ, LPZ, S-PPZ and PPZ was 56.6 ± 19.6, 53.1 ± 23.3, 35.6 ± 24.9 and 26.8 ± 30.2, respectively. The ΔTpH≥6 (%) was 50.7 ± 26.1, 41.4 ± 26.2, 25.4 ± 24.9 and 22.1 ± 27.6, respectively. The ΔAUECph-τ1-τ was 45,564 ± 16,107, 41,798 ± 16,153, 31,914 ± 17,304 and 20,744 ± 21,500, respectively. Statistically significant differences were found with R-LPZ vs. S-PPZ, R-LPZ vs. PPZ, LPZ vs. S-PPZ and LPZ vs. PPZ. The average TpH≥4 of R-LPZ, LPZ, S-PPZ, and PPZ was (47.2 ± 26.1) minutes, (49.6 ± 19.3) minutes, (56.1 ± 23.7) minutes, and (72.1 ± 27.3) minutes, respectively. Statistically significant differences were found with R-LPZ vs. PPZ (p = 0.009) and LPZ vs. PPZ (p = 0.019). After multiple doses, the ΔTpH≥4 (%) of R-LPZ, LPZ, S-PPZ, and PPZ was 71.7 ± 20.2, 63.5 ± 19.4, 59.5 ± 17.8 and 64.0 ± 22.4, respectively. The ΔTpH≥6 (%) was 64.0 ± 22.2, 52.0 ± 19.2, 49.6 ± 20.4 and 50.9 ± 23.8, respectively. The ΔAUECph-τ1-τ was 326,149 ± 94,839, 288,565 ± 93,279, 296,189 ± 83,412 and 300,960 ± 108,057, respectively. No statistically significant differences were found in baseline-adjusted PD parameters during all time periods after multiple doses. CONCLUSION: After a single dose, the mean gastric pH inhibition value of R-LPZ was the highest, followed by LPZ, then S-PPZ and PPZ. R-LPZ and LPZ provided significantly better pH control compared with PPZ and S-PPZ in healthy subjects. The onset time of R-LPZ was the fastest and R-LPZ can provide better acid inhibition during sleeping time. After multiple doses, the mean values in all PD parameters of R-LPZ were the highest, the values of LPZ, S-PPZ, and PPZ were similar. However, no significant difference was found in acid inhibition among these four drugs after multiple doses.


Assuntos
Antiulcerosos/farmacologia , Determinação da Acidez Gástrica , Lansoprazol/farmacologia , Pantoprazol/farmacologia , Voluntários Saudáveis , Humanos , Concentração de Íons de Hidrogênio , Estereoisomerismo
5.
Environ Pollut ; 252(Pt A): 336-343, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31158662

RESUMO

The health risks and toxicity of heavy metals (HMs) in PM2.5 are not only associated with their total amounts, but also with their species and bioaccessibility. In this study, the speciation (fractions) and bioaccessibility of HMs (Pb, Cd, Cr, Cu and Zn) as well as their correlations in fine particulate matter (PM2.5) samples from four seasons were studied. A sequential extraction procedure was applied to divide the studied HMs into four fractions: acid-soluble fraction (F1), reducible fraction (F2), oxidative fraction (F3) and residual fraction (F4). The simulated body fluids (gastrointestinal and lung phases) were used for in vitro tests in order to evaluate the bioaccessibility of HMs. The distribution of HMs in PM2.5 was season and element dependent. It was found that Zn was the most abundant element among the five measured metals and followed by Pb, Cu, Cr and Cd. The total contents of each HM in different seasons were in the following order: winter > autumn > spring > summer. The studied HMs were mainly concentrated in acid-soluble fraction (F1) with high bioaccessibility (p < 0.05) except for Cr. Zn, Pb and Cu possessed the highest bioaccessibility in summer while Cd and Cr were the highest in winter. In vitro tests indicated that HMs in PM2.5 were much more accessible to gastrointestinal fluids rather than lung phase (Gamble's solution). A significant correlation was found between the results from the optimized BCR sequential extraction and solubility bioaccessibility research consortium (SBRC). The fractions extracted by SBRC were consistent with the first two fractions extracted by the sequential extraction method.


Assuntos
Monitoramento Ambiental/métodos , Trato Gastrointestinal/química , Pulmão/química , Metais Pesados/análise , Material Particulado/análise , Líquidos Corporais/química , China , Cidades , Estações do Ano
6.
Ecotoxicol Environ Saf ; 169: 487-495, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30472473

RESUMO

Arsenic (As) can be easily enriched in atmospheric particulate matters (PMs), especially in fine particulate matters (PM2.5). In this study, thirty two PM2.5 samples were collected in four seasons in Baoding, China, where the haze pollution was very serious in recent years. The total contents, species and bioavailability of arsenic in PM2.5 samples were investigated. Species of arsenic in the PM2.5 samples were discriminated as five fractions using a sequential extraction method: non-specifically sorbed fraction (F1), specifically-sorbed fraction (F2), amorphous and poorly-crystalline hydrous oxides of Fe and Al fraction (F3), well-crystallized hydrous oxides of Fe and Al fraction (F4) and residual fraction (F5). Bioavailabilities of arsenic in the PM2.5 samples were evaluated by in vitro tests using both solubility bioavailability research consortium (SBRC) and Gamble's solution extraction methods. The total volume concentrations of As in PM2.5 were significantly higher in winter than the other seasons. However, the highest mass concentration of As was found in spring. Scanning electron microscopy (SEM) characterization indicated that the physical morphology of the particles varied in different seasons. Significant differences of fraction distribution and BFs were found between different seasons. Arsenic in PM2.5 samples mainly presented in F1 with high bioavailability factor (BF), especially for the samples in summer. In vitro tests indicated that arsenic in PM2.5 could be dissolved more easily in gastric phase rather than intestinal and lung phases. There was a significant correlation between species and in vitro tests. Interestingly, a synergy effect was found between F2 and F3. Health risk assessment indicated that arsenic in PM2.5via inhalation exposure for both children and adults could cause adverse effects. Principal component analysis suggested that the arsenic in PM2.5 was from the similar sources between summer and autumn, winter and spring, respectively.


Assuntos
Poluentes Atmosféricos/análise , Arsênico/análise , Monitoramento Ambiental/métodos , Exposição por Inalação/análise , Material Particulado/análise , Adulto , Disponibilidade Biológica , Criança , China , Cidades , Humanos , Tamanho da Partícula , Estações do Ano
7.
Chirality ; 30(12): 1277-1286, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30321480

RESUMO

Rabeprazole is an effective proton pump inhibitor to treat acid-related diseases. To achieve the simultaneous determination of rabeprazole enantiomers in human plasma, a chiral LC-MS/MS method was developed and validated. Acetonitrile including 0.1% ammonium were used as protein precipitating agent. Analytes were separated within 8 minutes on a Chiralpak IC column (4.6 mm × 150 mm, 5 µm). The mobile phase was 10 mM ammonium acetate including 0.2% acetic acid-acetonitrile (35:65, v/v). An API 4000 mass spectrometer was used as detector for the analysis, and the multiple reactions monitoring transitions of m/z 360.1 â†’ 242.2 and 346.1 â†’ 198.1 were opted for quantifying rabeprazole enantiomers and internal standard. Matrix effects were not apparent for each enantiomer and internal standard (esomeprazole), the calibration curves were linear over the concentration of 0.500 to 400 ng·mL-1 , the intra-run precisions were below 5.4%, the inter-run precisions were below 9.9%, and the accuracy was between -9.2% and 9.3%. There was no chiral inversion observed during sample storage, preparation procedure, and analysis, demonstrating that analytes were stable in this study. This method was applied to the stereoselective pharmacokinetic study of (R)-(+)- and (S)-(-)-rabeprazole after oral administration of 10-mg rabeprazole sodium enteric-coated tablet in healthy Chinese subjects.


Assuntos
Cromatografia Líquida/métodos , Rabeprazol/química , Rabeprazol/farmacocinética , Espectrometria de Massas em Tandem/métodos , Administração Oral , Adolescente , Adulto , Calibragem , Estabilidade de Medicamentos , Humanos , Limite de Detecção , Rabeprazol/administração & dosagem , Rabeprazol/sangue , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Estereoisomerismo , Comprimidos com Revestimento Entérico
9.
Fa Yi Xue Za Zhi ; 32(2): 105-8, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-27501681

RESUMO

OBJECTIVE: To explore the change rules of peak area ratio of STR loci to Amelogenin (AMEL) locus (STR/AMEL), a sex-determining gene in DNA degradation, and to evaluate the application of STR/AMEL value in the estimation of DNA degradation degree. METHODS: DNA was extracted from iliopsoas, and the variations of STR/AMEL value (Penta E/AMEL, Penta D/AMEL, FGA/AMEL) were analyzed after the artificial degradation was made by DNase I, and the changes of these three ratios of the iliopsoas naturally degraded in an outdoor environment were also analyzed. The regression curves were analyzed using the periods of DNA degradation and outside the body as the independent variable (x) and the STR/AMEL value as the dependent variable (y) and three curve equations under two conditions were established. RESULTS: Both under the conditions of artificial and natural degradation, STR/AMEL value had a negative relationship with the degradation time. The relationship between STR/AMEL and degradation time can be well simulated by the cubic function. R2 was over 0.99 under controlled degradation condition and over 0.86 under natural degradation condition. CONCLUSION: The STR/AMEL value (Penta E/AMEL, Penta D/AMEL, FGA/AMEL) is negatively related with the DNA degradation degree, which follows mathematical regression models strictly, and it might be applied to evaluate the DNA degradation degree.


Assuntos
Amelogenina/genética , Dano ao DNA/genética , Repetições de Microssatélites , Primers do DNA , Humanos , Análise de Regressão , Fatores de Tempo
10.
Artigo em Inglês | MEDLINE | ID: mdl-24708115

RESUMO

The complete mitochondrial genome of the Cecropis daurica was determined by using a PCR-based method. The complete mitochondrial DNA of this swallow was 17,949 bp in length, including 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and 2 control regions. All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The gene arrangement of the Cecropis daurica was similar to that found in other passerines. Base composition of the heavy strain was A (30.5%), T (23.0%), C (32.1%) and G (14.3%) with the A + T bias of 53.5%.


Assuntos
Genoma Mitocondrial/genética , Passeriformes/genética , Análise de Sequência de DNA , Animais , DNA Mitocondrial/genética , Genes de RNAr/genética , Anotação de Sequência Molecular , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , RNA de Transferência/genética
11.
Fa Yi Xue Za Zhi ; 31(3): 206-10, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26442374

RESUMO

OBJECTIVE: To analyze the cases of anaphylactic death cases and explore the standards of judicial expertise of anaphylactic death for providing evidence for judicial expertise. METHODS: Fifty-nine cases death due to allergic reaction in Shanghai were collected. And details of medical history, clinical manifestation of anaphylactic reaction and postmortem examination findings were reviewed for all cases. RESULTS: In the 59 cases, there were 58 cases died from drug allergy, including 77.6% of them were antibiotics. The rates of treating in standard hospital and illegal clinic were 37.3% and 61.0%, respectively. The allergic symptoms were dyspnea and facial cyanosis. The time from contacting allergens to death ranged from 1 min to 3 d. The concentration of total serum IgE ranged from 50 to 576.92 IU/mL. The results of clinical manifestation and pathological anatomy had obviously changes. CONCLUSION: Based on the exclusion of all other cause of death and synthetically analysis of details of cases, medical history, clinical manifestation and anatomy, the conclusion of anaphylactic death can reached. The details of cases including clinical history, exposure to allergens, and clinical manifestation play an important role in diagnosis of anaphylactic death.


Assuntos
Anafilaxia/mortalidade , Hipersensibilidade a Drogas/mortalidade , Antibacterianos/efeitos adversos , Autopsia , China , Ciências Forenses , Humanos
12.
Pathol Res Pract ; 211(6): 456-61, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25795229

RESUMO

Acute obstruction of coronary arteries leads to acute myocardial infarction (AMI), which causes unexpected death in humans. However, AMI cannot be easily detected in forensic examinations with traditional hematoxylin and eosin (H&E) staining. We analyzed whether cardiac troponin inhibitor (CTnI) could serve as a sensitive and specific early marker for diagnosing AMI in forensic medicine. We established an AMI model in rabbits by ligating the left ventricular branch and observed CTnI expression with immunohistochemistry after different ligation times. We found increased CTnI staining at the 0.5-h time point and depletion of CTnI staining with a 1-h ligation. The areas in which CTnI staining was depleted as seen with immunohistochemical analysis were consistent with the results of H&E staining. Next, human myocardium tissues from 30 persons who died from AMI and were subsequently examined in our forensic center were studied using immunohistochemistry with an antibody to human CTnI. Areas of infarction also showed depletion of CTnI staining. These findings suggested that immunohistochemical detection of CTnI is earlier, more sensitive, and myocardial tissue - specific as compared with H&E staining. CTnI may serve as an ideal marker for diagnosing AMI in forensic investigations.


Assuntos
Infarto do Miocárdio/metabolismo , Isquemia Miocárdica/metabolismo , Troponina/antagonistas & inibidores , Doença Aguda , Animais , Biomarcadores/metabolismo , Modelos Animais de Doenças , Humanos , Imuno-Histoquímica/métodos , Infarto do Miocárdio/diagnóstico , Coelhos
13.
Biopreserv Biobank ; 13(1): 31-6, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25686045

RESUMO

Genome-wide sequencing in glioma samples provides comprehensive insights into oncogenesis and malignant transformation. Several distinct biomarkers have been proven to have clinical significance and are being widely applied in routine clinical practice. Standard sample processing lays the foundation for successful molecular testing. In this study, we found intraoperative neuronavigation ensured higher tumor purity during sample collection, and an automated device helped improve DNA quality and increased yields. These two technologies are beneficial for glioma tissue bank construction and provide for accurate molecular testing during routine clinical practice.


Assuntos
Neoplasias Encefálicas/patologia , Glioma/patologia , Neuronavegação/métodos , Análise de Sequência de DNA/métodos , Bancos de Tecidos , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/cirurgia , DNA de Neoplasias/análise , Glioma/genética , Glioma/cirurgia , Humanos , Controle de Qualidade , Manejo de Espécimes/métodos
14.
Breast Cancer Res ; 16(3): R56, 2014 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-24886710

RESUMO

INTRODUCTION: Breast cancer is a worldwide health problem and the leading cause of cancer death among females. We previously identified Jumonji domain containing 2A (JMJD2A) as a critical mediator of breast cancer proliferation, migration and invasion. We now report that JMJD2A could promote breast cancer progression through transcriptional repression of the tumor suppressor aplasia Ras homolog member I (ARHI). METHODS: Immunohistochemistry was performed to examine protein expressions in 155 cases of breast cancer and 30 non-neoplastic tissues. Spearman correlation analysis was used to analyze the correlation between JMJD2A expression and clinical parameters as well as several tumor regulators in 155 cases of breast cancer. Gene and protein expressions were monitored by quantitative polymerase chain reaction (qPCR) and Western blot. Results from knockdown of JMJD2A, overexpression of JMJD2A, Co-immunoprecipitation (Co-IP) assay, dual luciferase reporter gene assay and chromatin immunoprecipitation (ChIP) elucidated molecular mechanisms of JMJD2A action in breast cancer progression. Furthermore, the effects of ARHI overexpression on JMJD2A-mediated tumor progression were investigated in vitro and in vivo. For in vitro experiments, cell proliferation, wound-healing, migration and invasion were monitored by cell counting, scratch and Boyden Chamber assays. For in vivo experiments, control cells and cells stably expressing JMJD2A alone or together with ARHI were inoculated into mammary fat pads of mice. Tumor volume, tumor weight and metastatic nodules were measured by caliper, electronic balance and nodule counting, respectively. RESULTS: JMJD2A was highly expressed in human breast cancers and positively correlated with tumor progression. Knockdown of JMJD2A increased ARHI expression whereas overexpression of JMJD2A decreased ARHI expression at both protein and mRNA levels. Furthermore, E2Fs and histone deacetylases were involved in the transcriptional repression of ARHI expression by JMJD2A. And the aggressive behavior of JMJD2A in breast cancers could be reversed by re-expression of ARHI in vitro and in vivo. CONCLUSION: We demonstrated a cancer-promoting effect of JMJD2A and defined a novel molecular pathway contributing to JMJD2A-mediated breast cancer progression.


Assuntos
Neoplasias da Mama/genética , Histona Desmetilases com o Domínio Jumonji/genética , Transcrição Genética/genética , Proteínas rho de Ligação ao GTP/biossíntese , Animais , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Progressão da Doença , Fatores de Transcrição E2F/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Genes Supressores de Tumor , Células HEK293 , Histona Desacetilases/genética , Humanos , Histona Desmetilases com o Domínio Jumonji/biossíntese , Células MCF-7 , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Invasividade Neoplásica/genética , Transplante de Neoplasias , Regiões Promotoras Genéticas/genética , Ligação Proteica/genética , Interferência de RNA , RNA Mensageiro/biossíntese , RNA Interferente Pequeno , Transplante Heterólogo , Cicatrização/genética , Proteínas rho de Ligação ao GTP/genética
15.
Mol Med Rep ; 10(1): 161-8, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24804616

RESUMO

MicroRNAs (miRNAs) comprise a broad class of small non­coding RNAs that control the expression of complementary target messenger RNAs. The dysregulation of miRNAs by several mechanisms has been described in various disease states, including cardiac disease. Although an etiological link between viral myocarditis (VMC) and idiopathic dilated cardiomyopathy (DCM) has long been recognized, the true extent of this association is uncertain. Previous studies of the two diseases have focused on protein degradation systems. In the present study, miR­21 expression and its potential role in VMC and DCM was investigated. The expression levels of miR­21, its target gene sprouty homolog 1 (SPRY1) and mitogen­activated protein kinase (MAPK) were measured by quantitative polymerase chain reaction. The protein levels of SPRY1 and MAPK were also determined by western blotting. miR­21 levels were significantly increased in cardiac myocytes from VMC and DCM in comparison with control samples. The levels of SPRY1 were decreased and MAPK activity was increased. Using a bioinformatics­based approach, an identical potential binding site was identified in mouse miR­21 and the SPRY 3' untranslated region (3' UTR), suggesting a regulatory role for miR­21. In cultured, miRNA­transfected myocardial cells, the overexpression of miR­21 was associated with a decrease in SPRY1 protein expression and an increased expression of the MAPK protein. These findings revealed that changes in the expression of miRNAs may contribute to the pathogenesis of VMC to DCM and establish the therapeutic efficacy of miRNA targeted intervention in a cardiovascular disease setting.


Assuntos
Cardiomiopatia Dilatada/metabolismo , MicroRNAs/metabolismo , Miocardite/metabolismo , Regiões 3' não Traduzidas , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Adulto , Animais , Cardiomiopatia Dilatada/patologia , Modelos Animais de Doenças , Progressão da Doença , Enterovirus Humano B/patogenicidade , Feminino , Humanos , Masculino , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Pessoa de Meia-Idade , Proteínas Quinases Ativadas por Mitógeno/genética , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Miocardite/patologia , Miocardite/virologia , Miocárdio/citologia , Miocárdio/metabolismo , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Adulto Jovem
16.
J Forensic Sci ; 59(5): 1286-94, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24611529

RESUMO

Determining the postmortem interval (PMI) is important in criminal, civil, and forensic cases. We examined the feasibility of using the transcript abundances of mRNAs, 18S rRNA, U6 snRNA, and microRNAs as a means to estimate the PMI. We removed spleen tissues from rats at different PMIs under 4°C or 25°C and examined gene transcript abundances in these samples by RT-qPCR. Using the algorithm geNorm, we found that microRNAs to be appropriate control markers because they were less affected by PMI and temperature. We also characterized relationships between observed PMI and the transcript levels of the above-mentioned RNAs. GAPDH1 and ACTB1 fluctuated slightly like cubic curves, while GAPDH2 and ACTB2 decreased rapidly. 18S rRNA transcript level exhibited a parabolic-like trend at 25°C and exponential growth at 4°C, while U6 transcript level exhibited exponential decay at 25°C and a parabolic-like trend at 4°C. Following validation, we conclude that GAPDH2, ACTB2, and 18S rRNA are suitable makers in the accurate determination of PMI.


Assuntos
MicroRNAs/metabolismo , Mudanças Depois da Morte , RNA Mensageiro/metabolismo , RNA Ribossômico 18S/metabolismo , RNA Nuclear Pequeno/metabolismo , Baço/metabolismo , Actinas/genética , Actinas/metabolismo , Animais , Estudos de Viabilidade , Marcadores Genéticos , Gliceraldeído-3-Fosfato Desidrogenase (Fosforiladora)/genética , Gliceraldeído-3-Fosfato Desidrogenase (Fosforiladora)/metabolismo , Masculino , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo
17.
Fa Yi Xue Za Zhi ; 30(5): 329-31, 2014 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-25735066

RESUMO

OBJECTIVE: To estimate the application of prognosis evaluation of ulnar nerve injury by 1H-magnetic resonance spectroscopy (1H-MRS). METHODS: The metabolites of first dorsal interossei (FDI) of two hands from 12 healthy volunteers and 1 volunteer with complete ulnar nerve injury were detected by 1H-MRS and the data were statistically analyzed. RESULTS: For the FDI of healthy adults, the female peaks area of extra-myocellular lipids (EMCL) was higher than the male (P < 0.05); There was no significant difference in Cho, Cr and intra-myocellular lipids (IMCL) between male and female (P > 0.05); There was no significant difference in all the peaks area between the left and right hand (P > 0.05). The EMCL peak of the injury side was higher than that of the healthy side, and the area of FDI was reduced in the volunteer with ulnar nerve injury. CONCLUSION: Noninvasive and quantitative detection of 1H-MRS may be valuable for prognosis evaluation of peripheral nerve injury.


Assuntos
Traumatismos dos Nervos Periféricos/diagnóstico , Espectroscopia de Prótons por Ressonância Magnética/métodos , Nervo Ulnar/lesões , Adulto , Feminino , Humanos , Masculino , Prognóstico , Distribuição por Sexo , Nervo Ulnar/metabolismo
18.
Fa Yi Xue Za Zhi ; 30(6): 409-12, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25816567

RESUMO

OBJECTIVE: To explore the relationship between the expression of EIIIA+ fibronectin in incised wound of rat's skin and injury time. METHODS: The wounding model was established by cutting the dorsal skin of 48 adult SD rats. The rats were sacrificed at the pre-set injury time as immediately, 0.5 h, 1 h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin samples were taken at the margin of wound. The expression of the EIIIA? fibronectin was detected by immunohistochemistry and Western blotting and the relationship be- tween its expression and injury time was observed. Results The expression of EIIIA+ fibronectin was not observed immediately. The basal cell of skin began to show positive expression 0.5 h after injury. With the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the Western blotting analysis. CONCLUSION: The expression of EIIIA+ fibronectin could be used for estimation of injury time in the early stage of skin injury.


Assuntos
Fibronectinas/metabolismo , Proteínas/metabolismo , Pele/metabolismo , Animais , Imuno-Histoquímica , Ratos , Ratos Sprague-Dawley , Pele/lesões , Coloração e Rotulagem , Fatores de Tempo
19.
Zhonghua Yi Xue Za Zhi ; 93(31): 2490-2, 2013 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-24300272

RESUMO

OBJECTIVE: To explore the efficacy and safety of megestrol acetate silicone vaginal ring. METHODS: A total of 165 healthy child-bearing age women were recruited to examine the efficacy, safety and satisfaction rate of megestrol acetate silicone vaginal ring. RESULTS: The cumulative pregnancy rate of megestrol acetate silicone vaginal ring was 3.93%. And its discontinuation rate was 2.54%, the incidence of adverse events 11.52% and the incidence of adverse effects 1.81% while the symptoms were all slight. The bleeding pattern was similar to that of normal menstruation. Compared with baseline, the elevated level of triglycerides and the reduced level of low-density lipoprotein (LDL) were significantly different while no significant difference existed in high-density lipoprotein (HDL), cholesterol and hemoglobin, etc. The satisfaction rate of megestrol acetate silicone vaginal ring was 94.6% and increased with time. CONCLUSION: Megestrol acetate silicone vaginal ring is an effective and safe option of contraceptives.


Assuntos
Dispositivos Anticoncepcionais Femininos , Acetato de Megestrol , Administração Intravaginal , Adulto , Dispositivos Anticoncepcionais Femininos/efeitos adversos , Feminino , Humanos , Acetato de Megestrol/efeitos adversos , Gravidez , Taxa de Gravidez , Adulto Jovem
20.
Fa Yi Xue Za Zhi ; 29(3): 164-7, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24303755

RESUMO

OBJECTIVE: To discuss the myocardial expression of Spry1 and MAPK proteins of viral myocarditis (VMC), to reveal its mechanism of sudden death, and to provide guides for forensic identification of sudden cardiac death. METHODS: Thirty Balb/c male mice were randomly divided into VMC group and control group, inoculated intraperitoneally with Coxsackievirus B3 and Eagel's solution, respectively. After the mice were sacrificed, the cardiac tissues of the mice were taken to proceed regular pathological examination. The changes of Spry1 protein, Spry1 mRNA and MAPK protein were detected by immunohistochemistry, Western blotting and real-time PCR. RESULTS: Under light microscope, the pathologic changes included myocardial interstitial edema, inflammatory cells infiltration, myocardial necrosis, and focal and patchy necrosis of myocardial fiber in VMC group. The expression of Spry1 protein in VMC group was lower than that in control group (P < 0.05). There was slightly decreased expression of Spry1 of the mRNA level in VMC group (P > 0.05). But the MAPK protein expression in VMC group was higher than that in control group (P < 0.05). CONCLUSION: The pathway of MAPK/ERK involving Spry1 protein accelerates the expression of collagen, which may contribute to arrhythmia, heart failure and even sudden cardiac death.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proteínas de Membrana/metabolismo , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Miocardite/metabolismo , Miocárdio/metabolismo , Fosfoproteínas/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Animais , Infecções por Coxsackievirus/metabolismo , Infecções por Coxsackievirus/patologia , Morte Súbita Cardíaca/patologia , Modelos Animais de Doenças , Imuno-Histoquímica , Masculino , Proteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Quinases Ativadas por Mitógeno/genética , Miocardite/patologia , Miocardite/virologia , Miocárdio/patologia , Fosfoproteínas/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Distribuição Aleatória , Reação em Cadeia da Polimerase em Tempo Real
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