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1.
Oxid Med Cell Longev ; 2020: 6181630, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32148653

RESUMO

Sleep deprivation adversely affects the digestive system. Multiple studies have suggested sleep deprivation and oxidative stress are closely related. Autophagy can be triggered by oxidative stress as a self-defense strategy to promote survival. In this study, we investigated the effects of sleep deprivation on liver functions, oxidative stress, and concomitant hepatocyte autophagy, as well as the associated pathways. Enzymatic and nonenzymatic biochemical markers in the serum were used to assess hepatic function and damage. To evaluate the occurrence of autophagy, expression of autophagy-related proteins was tested and autophagosomes were labeled. Additionally, methane dicarboxylic aldehyde (MDA), antioxidant enzymes, and the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway were analyzed using chemical methods and a Western blot. Serum alanine transaminase, aspartate aminotransferase, and alkaline phosphatase increased in sleep-deprived rats. Total protein and albumin abundance was also abnormal. Sleep deprivation induced histopathological changes in the liver. The superoxide dismutase level decreased significantly in the liver of sleep-deprived rats. In contrast, the MDA content increased in the sleep deprivation group. Moreover, the microtubule-associated protein 1 light chain 3 beta (LC3B) II/I ratio and Beclin I content increased considerably in the sleep-deprived rats, while p62 levels decreased. Sleep deprivation apparently inhibited the AKT/mTOR signaling pathway. We conclude that sleep deprivation can induce oxidative stress and ultimately cause liver injury. Autophagy triggered by oxidative stress appears to be mediated by the AKT/mTOR pathway and plays a role in relieving oxidative stress caused by sleep deprivation.

2.
Front Hum Neurosci ; 13: 366, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31736727

RESUMO

Electroencephalogram (EEG)-based affective brain-computer interfaces (aBCIs) have been attracting ever-growing interest and research resources. Whereas most previous neuroscience studies have focused on single-day/-session recording and sensor-level analysis, less effort has been invested in assessing the fundamental nature of non-stationary EEG oscillations underlying emotional responses across days and individuals. This work thus aimed to use a data-driven blind source separation method, i.e., independent component analysis (ICA), to derive emotion-relevant spatio-spectral EEG source oscillations and assess the extent of non-stationarity. To this end, this work conducted an 8-day music-listening experiment (i.e., roughly interspaced over 2 months) and recorded whole-scalp 30-ch EEG data from 10 subjects. Given the large size of the data (i.e., from 80 sessions), results indicated that EEG non-stationarity was clearly revealed in the numbers and locations of brain sources of interest as well as their spectral modulation to the emotional responses. Less than half of subjects (two to four) showed the same relatively day-stationary (source reproducibility >6 days) spatio-spectral tendency towards one of the binary valence and arousal states. This work substantially advances the previous work by exploiting intra- and inter-individual EEG variability in an ecological multiday scenario. Such EEG non-stationarity may inevitably present a great challenge for the development of an accurate, robust, and generalized emotion-classification model.

3.
Health Qual Life Outcomes ; 17(1): 89, 2019 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-31126289

RESUMO

BACKGROUND: Uterine fibroids (UFs) are the most common benign tumors in women. They are likely to cause numerous clinical symptoms, such as pain, menorrhagia, and other obstetric complications in pregnant women. This study aimed to determine the health-related quality of life (HRQoL) during pregnancy with uterine fibroids (UF), thus providing a utility-based case value in pregnant women with UF and understanding of whether HRQoL is associated with clinical outcomes in pregnant women with UFs. METHOD: This study was conducted in a cross-sectional manner. This study was based on questionnaire surveys completed by sequential out- and in-patients and was conducted in a regional university hospital in Guangzhou, China. The EuroQoL five-dimension-five-level (EQ-5D-5 L) questionnaire was used, and demographic data were collected. An electronic record of the clinical outcomes of pregnant women with UF was retrieved from the hospital's electronic medical record system. The association between UF and HRQoL was evaluated by ordered regression. RESULTS: Seven-hundred-sixty-seven pregnant women with a mean age (SD) of 32.7 (4.8) years completed 707 questionnaires. Overall, when comparing the UF with non-UF groups, we detected statistical differences in age, body mass index (BMI), gravidity and abortion times, partner's smoking and alcoholic habits, advanced maternal age, and uterine scars (p <  0.05). Furthermore, pregnant women without UF scored significantly higher than those with UF on the EQ-5D value system (0.84 versus 0.79; p = 0.017). Moreover, pregnant women with UF suffered more health-related problems, especially with respect to self-care (odds ratio [OR] = 3.69, p <  0.01) and usual activity dimensions (OR = 2.11; p = 0.01). CONCLUSION: We found that UF has a negative impact on the HRQoL of pregnant women with respect to self-care and usual activity dimensions. Also, the EQ-5D score was a better index than the EQ-VAS score for HRQoL when evaluating of the QoL of our population of pregnant women.


Assuntos
Leiomioma/psicologia , Complicações Neoplásicas na Gravidez/psicologia , Qualidade de Vida , Neoplasias Uterinas/psicologia , Adulto , Estudos de Casos e Controles , China , Estudos Transversais , Feminino , Humanos , Gravidez , Autocuidado/psicologia , Inquéritos e Questionários
4.
J Cell Biochem ; 120(8): 12500-12507, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30834595

RESUMO

Radiation-induced lung injury (RILI) frequently occurs in patients with thoracic malignancies. In response to radiation, alveolar epithelial cells (AEC) undergo epithelial-mesenchymal transition (EMT) and contribute to the pathogenesis of RILI. Insulin-like growth factor binding protein 7 (IGFBP7) is reported as a downstream mediator of transforming growth factor-ß1 (TGF-ß1) pathway, which plays a crucial role in radiation-induced EMT. In the present study, the levels of IGFBP7 and TGF-ß1 were simultaneously increased in experimental RILI models and radiation-treated AEC (human pulmonary alveolar epithelial cells [HPAEpic]). The expression of IGFBP7 in radiation-treated HPAEpic cells was obviously inhibited by the specific inhibitor of TGF-ß receptor antagonist SB431542 and TGF-ß1 neutralizing antibody, and time-dependently enhanced by TGF-ß1 treatment. Moreover, IGFBP7 knockdown significantly attenuated the effects of radiation on morphology change, cell migration, expression of EMT-related markers (E-cadherin, α-SMA, and Vimentin), and phosphorylation of extracellular-signal-regulated kinase (ERK). The effects of IGFBP7 overexpression on the expression of EMT-related markers were partially reversed by the ERK inhibitor PD98059. In conclusion, IGFBP7, was enhanced by TGF-ß1, may be involved in radiation-induced EMT of AEC via the ERK signaling pathway, thus contributing to the pathogenesis of RILI.

5.
Sci Rep ; 8(1): 10754, 2018 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-30018382

RESUMO

Itching is a leading symptom of eczema or dermatitis and has a great impact on patients' lives. Previous studies on itching have focused mostly on atopic dermatitis (AD). A cross-sectional multicenter study was conducted among outpatients with eczema from 39 tertiary hospitals in mainland China from July 1 to September 30, 2014. This work elaborates on itching in different types of eczema. Itching was very common (97%, 8499/8758) in outpatients with eczema. The severity of the itch increased with age and disease duration (P < 0.001). The top three subtypes of dermatitis with severe itching were atopic dermatitis (30.4%), widespread eczema (30.1%), and asteatotic eczema (27.9%). Widespread eczema refers to the involvement of more than three body parts, without clinical features of other specific types of eczema. The proportion of outpatients without itching was highest in hand eczema (6.8%). Positive correlations were observed between the severity of itching and the proportions of different diseases based on trend tests, including atopic dermatitis (P < 0.001), widespread eczema (P < 0.001), asteatotic eczema (P < 0.001), and autosensitization dermatitis (P < 0.001). Eczema outpatients with older age, longer disease duration, and, especially, a history of allergic diseases might be more prone to itching.


Assuntos
Dermatite/complicações , Eczema/complicações , Prurido/epidemiologia , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Criança , China , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prurido/complicações , Centros de Atenção Terciária
6.
J Ethnopharmacol ; 217: 126-133, 2018 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-29454912

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Bidens pilosa L, belonging to the family of Acanthaceae, has been used as an anticancer medicine in folk in China. In our preliminary experiments, the petroleum ether extract from B. pilosa showed good cytotoxic activity to human lung cancer A549 cell. However, to date, it's lack of the further study on antitumor effect, mechanism and active substances composition of the petroleum ether extract of B. pilosa. AIM OF THE STUDY: The study aimed to evaluate the anti-lung cancer efficacy of the petroleum ether extract from B. pilosa (PEEBP) in vitro and in vivo, explore the possible anticancer mechanisms, and further disclose the chemical composition of the extract. MATERIALS AND METHODS: B. pilosa was extracted with 75% ethanol (v/v), followed by extracted with petroleum ether to obtain the objective fraction. Antiproliferation effect of the petroleum ether extract in HepG2, A549, CNE and B16 cells was evaluated by MTT assay. The in vivo anticancer effect was examined by A549 cells nude mice xenograft tumor model. The possible effect mechanism was studied by western blot assay. The chemical constituents of the extract was analyzed by GC-MS. RESULTS: The petroleum ether extract showed favorable antiproliferation activity against the four human cancer cell lines, especially for A549 cells with an IC50 of 49.11 ±â€¯2.72 µg/mL. The extract inhibited the growth of A549 cell in mice with the inhibitory rates of 24.76%, 35.85% and 53.07% for 90, 180 and 360 mg/kg oral dosages, respectively. The B. pilosa extract could significantly down-regulate the expression of apoptosis-related protein Bcl-2 and up-regulate the protein expression of Bax and Caspase-3. 138 compounds were identified by GC-MS in the extract and the main chemical components were triterpenes, including 4,22-cholestadien-3-one (4.82%), stigmasterol (4.56%), friedelan-3-one (3.28%), etc. CONCLUSION: The PEEBP is abundant of triterpenes and has significant anti-tumor activities against human A549 cells in vitro and in vivo, indicating it a potential anticancer agent.


Assuntos
Alcanos/química , Antineoplásicos Fitogênicos/farmacologia , Bidens , Medicamentos de Ervas Chinesas/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Neoplasias Pulmonares/tratamento farmacológico , Solventes/química , Triterpenos/farmacologia , Células A549 , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Proteínas Reguladoras de Apoptose/metabolismo , Bidens/química , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/isolamento & purificação , Células Hep G2 , Humanos , Concentração Inibidora 50 , Neoplasias Pulmonares/patologia , Masculino , Melanoma Experimental/tratamento farmacológico , Melanoma Experimental/patologia , Camundongos Endogâmicos BALB C , Camundongos Nus , Fitoterapia , Plantas Medicinais , Fatores de Tempo , Triterpenos/isolamento & purificação , Ensaios Antitumorais Modelo de Xenoenxerto
7.
G Ital Dermatol Venereol ; 153(3): 389-395, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29064205

RESUMO

INTRODUCTION: Despite the recent studies revealed Vitamin D may play a role in urticaria, no meta-analysis providing definite conclusions in this field has been reported. EVIDENCE ACQUISITION: A systematic search up to June 30th, 2017 was conducted in MEDLINE (via PubMed) and Excerpta Medica database (EMBASE) to identify relevant articles. Search terms included ''vitamin D'' or "25-hydroxyvitamin D" or "25-hydroxy-vitamin D" or "25(OH)D" or "1,25-dihydroxyvitamin D" combined with ''urticaria." EVIDENCE SYNTHESIS: Initial searches yielded 30 citations. Of these original results, 10 met specific selection criteria and were included in the meta-analysis with 547 urticaria cases and 517 controls. Vitamin D levels were significantly decreased in the urticarial cases (WMD -9.8 mg/L, 95% CI: -12.57 to -7.04 mg/L, from a random effects model), with a significant heterogeneity (I2=85.9%, P=0.000). When divide the Vitamin D level into two subgroups, the pooled OR comparing vitamin D insufficiency vs. normal level was 1.64 (95% CI: 0.71 to 3.77). Meanwhile, vitamin D deficiency vs. normal level was 4.09 (95% CI: 1.32 to 12.73). CONCLUSIONS: The study demonstrated that patients with urticaria had a lower Vitamin D level than healthy controls. Vitamin D deficiency rather than insufficiency has a positive relationship with urticaria. However, larger-scale studies with a longer duration of treatment are needed to confirm this conclusion.


Assuntos
Urticária/epidemiologia , Deficiência de Vitamina D/epidemiologia , Vitamina D/análogos & derivados , Humanos , Urticária/sangue , Vitamina D/sangue
8.
Medicine (Baltimore) ; 96(35): e7955, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28858126

RESUMO

Little is known about the classification and bacterial infection in outpatients with eczema and dermatitis in China.To investigate the prevalence of eczema and dermatitis in outpatients of dermatology clinics in China, examine classification and proportion of common types of dermatitis and the possible bacterial infection, and analyze the possible related factors.Outpatients with eczema or dermatitis from 39 tertiary hospitals of 15 provinces in mainland China from July 1 to September 30, 2014, were enrolled in this cross-sectional and multicenter study. Among 9393 enrolled outpatients, 636 patients (6.7%) were excluded because of incomplete information.The leading subtypes of dermatitis were unclassified eczema (35.5%), atopic dermatitis (13.4%), irritant dermatitis (9.2%), and widespread eczema (8.7%). Total bacterial infection rate was 52.3%, with widespread eczema, stasis dermatitis, and atopic dermatitis being the leading three (65.7%, 61.8%, and 61.4%, respectively). Clinically very likely bacterial infection has a significant positive correlation with disease duration, history of allergic disease, history of flexion dermatitis, and severe itching.Atopic dermatitis has become a common subtype of dermatitis in China. Secondary bacterial infection is common in all patients with dermatitis, and more attentions should be paid on this issue in other type of dermatitis apart from atopic dermatitis.


Assuntos
Dermatite Atópica/classificação , Dermatite Atópica/microbiologia , Eczema/classificação , Eczema/microbiologia , Dermatopatias Bacterianas/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , China/epidemiologia , Estudos Transversais , Dermatite Atópica/epidemiologia , Eczema/epidemiologia , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Pacientes Ambulatoriais , Prevalência , Dermatopatias Bacterianas/tratamento farmacológico , Dermatopatias Bacterianas/epidemiologia , Infecções Cutâneas Estafilocócicas/diagnóstico , Infecções Cutâneas Estafilocócicas/tratamento farmacológico , Infecções Cutâneas Estafilocócicas/epidemiologia , Adulto Jovem
9.
Medicine (Baltimore) ; 96(11): e6317, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28296746

RESUMO

The prevalence of atopic dermatitis (AD) has increased substantially. Previous studies have focused mostly on pediatric patients, while epidemiological investigation on adult AD has been very limited.The aim of this study was to determine the prevalence and clinical features of adult AD in outpatients with dermatitis and eczema in China mainland.A multicenter cross-sectional study was conducted among outpatients with eczema or dermatitis from 39 tertiary hospitals of 15 provinces in China from July 1 to September 30, 2014.Of 8758 patients, 407 were adult AD. Compared with adults with other types of dermatitis, the mean age (41.8 ±â€Š14.3 vs 42.04 ±â€Š15.38 years, P < 0.05) and onset age (35.2 ±â€Š11.2 vs 39.2 ±â€Š14.0 years, P < 0.001) of adult AD were younger, and mean disease duration was longer (5.3 ±â€Š7.1 vs 2.8 ±â€Š4.9 years, P < 0.001). About 53.3% adult AD involved 3 or more body locations, higher than adults with other types of dermatitis (34.4%, P < 0.001), but lower than those with pediatric and adolescent AD (73.8%, P < 0.001). History of asthma (19.2% vs 6.9%, P < 0.001) or allergic conjunctivitis (21.9% vs 14.9%, P < 0.05) was more common in adult AD than pediatric/adolescent AD. Suspected bacterial infection was more frequently in adult AD than adults with other types of dermatitis (24.3% vs 14.6%, P < 0.001) and pediatric/adolescent AD (24.3% vs 14.9%, P < 0.001). More severe itching was observed in 31.4% of adult AD, higher than that of adults with other types of dermatitis (15.4%, P < 0.001), whereas similar to that of pediatric/adolescent AD (28.7%, P > 0.05). The highest (8.7%) and lowest prevalence (3.7%) of adult AD were in 25°N to 30°N and 35°N to 40°N latitude region.A substantial part of adult outpatients with eczema or dermatitis is adult AD. Middle age, more body location involvement, more suspected bacterial infection, and severe itching are the main clinical feathers of adult AD. Geographical environment and economic situation work in synergy to adult AD.


Assuntos
Dermatite Atópica/epidemiologia , Dermatite Atópica/fisiopatologia , Adolescente , Adulto , Fatores Etários , Idade de Início , Asma/epidemiologia , China/epidemiologia , Conjuntivite Alérgica/epidemiologia , Estudos Transversais , Dermatite/epidemiologia , Eczema/epidemiologia , Meio Ambiente , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Índice de Gravidade de Doença , Fatores Socioeconômicos , Centros de Atenção Terciária/estatística & dados numéricos , Adulto Jovem
10.
Zhonghua Nan Ke Xue ; 23(8): 687-691, 2017 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-29726641

RESUMO

Objective: To investigate the influence of deep slow-wave sleep deprivation on the oxidative stress of testicular tissue in rats. METHODS: Thirty-six 5-week-old male Wistar rats were equally randomized into deep slow-wave sleep deprivation group (SD1), deep slow-wave sleep and duration sleep deprivation group ( SD2), and a cage control group (CC). The rat model of deep slow-wave sleep deprivation was established using the flowerpot technique. The rats in the SD1 group were interfered every 24 minutes and deprived of 12 hours of sleep at night, those in the SD2 group deprived of 8 minutes of sleep at an interval of 24 minutes and 12 hours of sleep at night, and those in the CC group exposed to 12 hours of daylight and 12 hours of darkness. After 28 days, all the rats were executed for measurement of the testis volume and protein content, determination of the methane dicarboxylic aldehyde (MDA) level and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and observation of the pathological changes in the testicular tissue under the microscope. RESULTS: Compared with the CC group, the rats in the SD1 and SD2 groups showed significantly reduced body weight (ï¼»268.5 ± 1.6ï¼½ vs ï¼»248.1 ± 25.1ï¼½andï¼»232.9 ± 10.1ï¼½g, P<0.05) and increased relative testis mass (ï¼»50.0 ± 1.3ï¼½ vs ï¼»57.9 ± 6.1ï¼½ and ï¼»54.9 ± 3.5ï¼½ ×10⁻², P<0.05). Statistically significant differences were found between the CC and SD2 groups in the contents of protein (ï¼»6.3 ± 1.4ï¼½ vs ï¼»4.5 ± 0.9ï¼½ gpro/L, P<0.05) and MDA (ï¼»1.1 ± 0.1ï¼½ vs ï¼»1.3 ± 0.3ï¼½ nmol/mgpro, P<0.05) and the activities of SOD (ï¼»104.3 ± 33.1ï¼½ vs ï¼»135.2 ± 26.9ï¼½ U/mgpro, P<0.05) and GSH-Px (ï¼»15.6 ± 4.0ï¼½ vs ï¼»21.7 ± 4.3ï¼½ U/mgpro, P<0.05), but not between the CC and SD1 groups (P>0.05). The lumens in the testis were narrowed, with obvious hyperplasia, hyperemia and edema in the peripheral interstitial tissue, but no significant pathologic changes were observed in the testis tissue of the SD1 group. CONCLUSIONS: Long-term deprivation of deep slow-wave sleep impairs the structure of the testis tissue and induces oxidative stress response in rats.


Assuntos
Estresse Oxidativo , Privação do Sono/metabolismo , Testículo/metabolismo , Testículo/patologia , Animais , Peso Corporal , Glutationa Peroxidase/análise , Masculino , Malondialdeído , Distribuição Aleatória , Ratos , Ratos Wistar , Fases do Sono , Superóxido Dismutase/análise , Fatores de Tempo , Perda de Peso
11.
Biomed Res Int ; 2016: 2568301, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27957490

RESUMO

Background. The epidemiology of atopic dermatitis (AD) in Chinese outpatients is yet to be clarified. Objectives. To investigate population-based prevalence and clinical features of AD in Chinese outpatients. Methods. A multicenter cross-sectional study was conducted in outpatients with eczema or dermatitis from 39 tertiary hospitals in 15 provinces. Results. This study included 682 patients diagnosed with AD, with the mean age of 28.8 ± 20.1 years and the median course of 5.3 ± 6.9 years. AD patients had more severe itching (30.4% versus 13.8%, p < 0.001) and clinically suspected bacterial infection (21.7% versus 16.1%, p < 0.001) than those of other types of dermatitis. Older patients were more susceptible to have a history of flexion dermatitis (p < 0.001), bacterial infection (p = 0.005), and severe itching (p < 0.001). Outpatients with clinically suspected bacterial infection had 3.53-fold increased risk of AD than those without it (p < 0.001). The morbidity rate of AD in the (20-25°N) region is 2.86 times higher than that in the (40-45°N) region [OR (95% CI): 0.352 (0.241-0.514), p < 0.001]. Conclusions. AD is characterized by unique clinical/demographic features. Bacterial infection and latitude region may have an impact on the incidence of AD in China.


Assuntos
Dermatite Atópica , Adolescente , Adulto , Criança , China/epidemiologia , Estudos Transversais , Dermatite Atópica/diagnóstico , Dermatite Atópica/epidemiologia , Dermatite Atópica/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Adulto Jovem
12.
Med Phys ; 41(4): 042901, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24694157

RESUMO

PURPOSE: A computer-aided detection (CADe) system based on quantitative tissue clustering algorithm was proposed to identify potential tumors in automated breast ultrasound (ABUS) images. METHODS: Our three-dimensional (3D) ABUS images database included 148 biopsy-verified lesions (size 0.4-7.9 cm; mean 1.76 cm). An ABUS volume was comprised of 229-282 slices of two-dimensional (2D) images. For tumor detection, the fast 3D mean shift method was used to remove the speckle noise and the segment tissues with similar properties. The hypoechogenic regions, i.e., the tumor candidates, were extracted using fuzzy c-means clustering. Seven features related to echogenicity and morphology were quantified and used to predict the likelihood of identifying a tumor and filtering out the false-positive (FP) regions. RESULTS: The sensitivity of the proposed CADe system achieved 89.19% (132/148) with 2.00 FPs per volume. For the volumes without lesion, the FP rate was 1.27. The sensitivity was 92.50% (74/80) for malignant tumors and 85.29% (58/68) for benign tumors. CONCLUSIONS: The proposed CADe system provides an automatic and quantitative procedure for tumor detection in ABUS images. Further studies are needed to reduce the FP rate of the CADe algorithm.


Assuntos
Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/patologia , Mama/patologia , Interpretação de Imagem Assistida por Computador/métodos , Ultrassonografia Mamária/métodos , Adulto , Idoso , Algoritmos , Automação , Análise por Conglomerados , Reações Falso-Positivas , Feminino , Humanos , Pessoa de Meia-Idade , Curva ROC , Estudos Retrospectivos , Adulto Jovem
13.
Ultrason Imaging ; 36(1): 3-17, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24275536

RESUMO

Automated whole breast ultrasound (ABUS) has become a popular screening tool in recent years. To reduce the review time and misdetection from ABUS images by physicians, a computer-aided detection (CADe) system for ABUS images based on a multiview method is proposed in this study. A total of 58 pathology-proven lesions from 41 patients were used to evaluate the performance of the system. In the proposed CADe system, the fuzzy c-mean clustering method was applied to detect tumor candidates from these ABUS images. Subsequently, the tumor likelihoods of these candidates could be estimated by a logistic linear regression model based on the intensity, morphology, location, and size features in the transverse, longitudinal, and coronal views. Finally, the multiview tumor likelihoods of the tumor candidates could be obtained from the estimated tumor likelihoods of the three views, and the tumor candidates with high multiview tumor likelihoods were regarded as the detected tumors in the proposed system. The sensitivities of the multiview tumor detection for selecting 5, 10, 20, and 30 tumor candidates with the largest multiview tumor likelihoods were 79.31%, 86.21%, 96.55%, and 98.28%, respectively.


Assuntos
Neoplasias da Mama/diagnóstico por imagem , Processamento de Imagem Assistida por Computador/métodos , Ultrassonografia Mamária/métodos , Análise por Conglomerados , Feminino , Lógica Fuzzy , Humanos , Processamento de Imagem Assistida por Computador/estatística & dados numéricos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Ultrassonografia Mamária/estatística & dados numéricos
14.
J ECT ; 29(4): 297-302, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24263275

RESUMO

OBJECTIVES: This study aimed at investigating the effects of propofol on the expression of hippocampal neuronal nitric oxide synthase (nNOS) and carboxy-terminal PDZ ligand of nNOS (CAPON) in stressed rats after electroconvulsive shock (ECS). METHODS: Sprague-Dawley rats were stressed repeatedly for 28 days to establish a stressed model. Forty stressed rats were randomly divided into 4 groups (n = 10 for each group): the stressed group (unapplied group), propofol group (applied with intraperitoneal injection of propofol 80 mg/kg for 7 days), ECS group (applied with ECS and intraperitoneal injection of normal saline 8 mL/kg), or propofol + ECS group (applied with ECS and intraperitoneal injection of propofol 80 mg/kg). Sucrose preference test, open-field test, and Morris water maze were used to assess behavioral changes. Immunohistochemistry was carried out to measure the expression of nNOS and CAPON in hippocampal CA1, CA3, and DG areas. RESULTS: Rats in the ECS and propofol + ECS groups had higher sucrose preference percentages and open-field scores when compared with the stressed group. Rats in the ECS group exhibited longer escape latency, shorter space exploration time, up-regulated expression of nNOS, down-regulated expression of CAPON, and increased ratio of nNOS/CAPON in hippocampus. Compared with the ECS group, the propofol + ECS group exhibited shorter escape latency, longer space exploration time, down-regulated expression of nNOS, up-regulated expression of CAPON in hippocampus, and lower nNOS/CAPON values. CONCLUSIONS: Propofol may alleviate ECS-induced impairment of learning and memory in stressed rats by inhibiting excessive expression of nNOS and enhancing the expression of CAPON to maintain the balance between nNOS and CAPON in hippocampus.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Eletrochoque/métodos , Hipocampo/efeitos dos fármacos , Óxido Nítrico Sintase Tipo I/metabolismo , Propofol/farmacologia , Animais , Transtorno Depressivo/tratamento farmacológico , Transtorno Depressivo/metabolismo , Transtorno Depressivo/terapia , Modelos Animais de Doenças , Comportamento Exploratório/efeitos dos fármacos , Preferências Alimentares/efeitos dos fármacos , Hipocampo/enzimologia , Hipnóticos e Sedativos/farmacologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Transtornos da Memória/diagnóstico , Transtornos da Memória/etiologia , Óxido Nítrico/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Estresse Psicológico/tratamento farmacológico , Estresse Psicológico/metabolismo
15.
IEEE Trans Med Imaging ; 32(7): 1191-200, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23232413

RESUMO

Automated whole breast ultrasound (ABUS) is an emerging screening tool for detecting breast abnormalities. In this study, a computer-aided detection (CADe) system based on multi-scale blob detection was developed for analyzing ABUS images. The performance of the proposed CADe system was tested using a database composed of 136 breast lesions (58 benign lesions and 78 malignant lesions) and 37 normal cases. After speckle noise reduction, Hessian analysis with multi-scale blob detection was applied for the detection of tumors. This method detected every tumor, but some nontumors were also detected. The tumor like lihoods for the remaining candidates were estimated using a logistic regression model based on blobness, internal echo, and morphology features. The tumor candidates with tumor likelihoods higher than a specific threshold (0.4) were considered tumors. By using the combination of blobness, internal echo, and morphology features with 10-fold cross-validation, the proposed CAD system showed sensitivities of 100%, 90%, and 70% with false positives per pass of 17.4, 8.8, and 2.7, respectively. Our results suggest that CADe systems based on multi-scale blob detection can be used to detect breast tumors in ABUS images.


Assuntos
Neoplasias da Mama/diagnóstico por imagem , Interpretação de Imagem Assistida por Computador/métodos , Ultrassonografia Mamária/métodos , Algoritmos , Mama/patologia , Neoplasias da Mama/patologia , Bases de Dados Factuais , Feminino , Humanos
16.
Acta Derm Venereol ; 93(1): 62-5, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22859054

RESUMO

Vitiligo is a common skin disease, the prevalence of which varies between races and countries. In China, no population-based study has been reported, although there have been some epidemiological studies on single cities or regions. The objective of this study was to obtain the prevalence and clinical profile of vitiligo in China. The study was conducted in 6 cities. Cluster sampling was used in selecting communities. Residents were visited at home and were asked to complete questionnaires and receive dermatological examinations. A total of 19,974 residents were visited and 17,345 valid questionnaires were obtained. The overall prevalence of vitiligo was 0.56%. Men were affected more than women (0.71% vs. 0.45%, p < 0.01). The prevalence of vitiligo increased with age. The most common type was focal vitiligo (36.1%). A positive family history was found in 9.8% of patients. Thirty-two percent of patients reported a negative impact of vitiligo on their quality of life.


Assuntos
Vitiligo/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Idade de Início , Idoso , Criança , Pré-Escolar , China/epidemiologia , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Prevalência , Qualidade de Vida , Amostragem , Distribuição por Sexo , Vitiligo/genética , Vitiligo/psicologia , Adulto Jovem
17.
Eur J Dermatol ; 22(5): 663-7, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22910173

RESUMO

BACKGROUND: Although psoriasis occurs worldwide, the prevalence varies considerably between different peoples and regions. In China, a questionnaire-based study was carried out in 1987 and the prevalence of psoriasis was found to be 0.12%. Since then, no large-scale, population-based study has been reported. OBJECTIVES: To obtain the accurate figures for the prevalence of psoriasis in China. METHODS: A population-based survey was conducted in 6 cities. The cluster sampling method was used to select communities in each city. The subjects were required to fill out self-reporting questionnaires during a face-to-face interview and also received physical examination by dermatologists. RESULTS: 19,974 subjects were visited and 17,345 completed the questionnaires and received dermatological examination. 102 subjects (0.59%) were found to have psoriasis. After standardization, the prevalence of psoriasis was 0.47%. The prevalence of psoriasis in males and females was 0.54% and 0.44% respectively. 97.06% of the patients had psoriasis vulgaris. 28.43% of the patients reported a family history of psoriasis. 59.80% of patients experienced a negative influence on the quality of life. CONCLUSIONS: This population-based and dermatologist-confirmed study showed that the prevalence of psoriasis in China is 0.47%, which is higher than that reported in 1987.


Assuntos
Psoríase/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , China/epidemiologia , Feminino , Inquéritos Epidemiológicos , Humanos , Lactente , Entrevistas como Assunto , Masculino , Pessoa de Meia-Idade , Exame Físico , Prevalência , Psoríase/genética , Qualidade de Vida , Inquéritos e Questionários , Adulto Jovem
18.
Acta Derm Venereol ; 92(1): 40-4, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21710106

RESUMO

Acne vulgaris is a common skin condition in adolescents. The prevalence of acne is thought to vary between ethnic groups and countries. A large-scale community-based study was performed in six cities in China to determine the prevalence and possible risk factors for acne in the Chinese population. A total of 17,345 inhabitants were included in this study. Of these, 1,399 were found to have acne. No acne was found in subjects under 10 years of age, and only 1.6% in the 10-year-old group had acne. Prevalence then increased rapidly with age, up to 46.8% in the 19-year-old group. After that, it declined gradually with age. Acne was rare in people over 50 years of age. In subjects in their late teens and 20s, acne was more prevalent in males, while in those over 30 years of age it was more prevalent in females. In subjects with acne, 68.4% had mild; 26.0% had moderate and 5.6% had severe acne. In adult acne, persistent acne was much more common (83.3%) than late-onset acne (16.7%). Smoking and drinking were found to be associated with adolescent acne, while no association was found between diet and acne. These results suggest that the prevalence of acne in the Chinese population is lower than that in Caucasian populations, and that adult acne is not uncommon in Chinese subjects.


Assuntos
Acne Vulgar/epidemiologia , Adolescente , Adulto , Fatores Etários , Consumo de Bebidas Alcoólicas , China/epidemiologia , Dieta , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Índice de Gravidade de Doença , Fumar , Adulto Jovem
19.
Ultrasound Med Biol ; 37(4): 539-48, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21420580

RESUMO

New automated whole breast ultrasound (ABUS) machines have recently been developed and the ultrasound (US) volume dataset of the whole breast can be acquired in a standard manner. The purpose of this study was to develop a novel computer-aided diagnosis system for classification of breast masses in ABUS images. One hundred forty-seven cases (76 benign and 71 malignant breast masses) were obtained by a commercially available ABUS system. Because the distance of neighboring slices in ABUS images is fixed and small, these continuous slices were used for reconstruction as three-dimensional (3-D) US images. The 3-D tumor contour was segmented using the level-set segmentation method. Then, the 3-D features, including the texture, shape and ellipsoid fitting were extracted based on the segmented 3-D tumor contour to classify benign and malignant tumors based on the logistic regression model. The Student's t test, Mann-Whitney U test and receiver operating characteristic (ROC) curve analysis were used for statistical analysis. From the Az values of ROC curves, the shape features (0.9138) are better than the texture features (0.8603) and the ellipsoid fitting features (0.8496) for classification. The difference was significant between shape and ellipsoid fitting features (p = 0.0382). However, combination of ellipsoid fitting features and shape features can achieve a best performance with accuracy of 85.0% (125/147), sensitivity of 84.5% (60/71), specificity of 85.5% (65/76) and the area under the ROC curve Az of 0.9466. The results showed that ABUS images could be used for computer-aided feature extraction and classification of breast tumors.


Assuntos
Algoritmos , Inteligência Artificial , Neoplasias da Mama/diagnóstico por imagem , Interpretação de Imagem Assistida por Computador/métodos , Reconhecimento Automatizado de Padrão/métodos , Ultrassonografia Mamária/métodos , Adulto , Idoso , Feminino , Humanos , Aumento da Imagem/métodos , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Adulto Jovem
20.
Med Phys ; 38(1): 382-9, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21361206

RESUMO

PURPOSE: The purpose of this study is to compare the measurements of breast density using three-dimensional (3-D) automated whole breast ultrasound (ABUS) and magnetic resonance imaging (MRI). METHODS: In this study, 3-D ABUS and MRI breast images were obtained from 40 patients-bilaterally in 27 patients and unilaterally (due to operation in the contralateral breast) in 13 patients, To differentiate the fibroglandular and fatty tissues in ABUS and MRI images, the fuzzy C-mean classifier was used. Calculated values for percent density and breast volume from the two modalities were compared to and correlated with linear regression analysis. Intraoperator and interoperator variations among eight cases were evaluated to verify the consistency of the density analysis. RESULTS: Mean percent density and breast volume derived from ABUS (17.63 +/- 11.87% and 418.30 +/- 132.97 cm3, respectively) and MRI images (23.79 +/- 16.62% and 544.90 +/- 207.41 cm3) demonstrated good correlation (R = 0.917 and R = 0.884). Intraoperator and interoperator analyses yielded slightly larger coefficients of variation for percent density and breast volume in ABUS compared to MRI. However, the differences were not statistically significant. CONCLUSIONS: ABUS and MRI showed high correlation for breast density and breast volume quantification. Both modalities could provide useful breast density information to physicians.


Assuntos
Mama/citologia , Imageamento Tridimensional/métodos , Imagem por Ressonância Magnética/métodos , Ultrassonografia Mamária/métodos , Tecido Adiposo/metabolismo , Automação , Mama/anatomia & histologia , Humanos , Pessoa de Meia-Idade , Variações Dependentes do Observador , Tamanho do Órgão
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