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Ying Yong Sheng Tai Xue Bao ; 26(11): 3285-92, 2015 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-26915181


Based on a natural Larix gmelinii forest from Mohe Ecological Station, located in north of Great Xing' an Mountains, time lag effects of throughfall inside the Larix gmelinii forest were analyzed by measuring rainfall, throughfall and stemflow with the method of location observation. The result showed that forest throughfall, stemflow and canopy interception accounted for 76.5%, 2.6% and 20.9% of total rainfall, respectively. Time lag of rainfall inside L. gmelinii forest was found both in beginning and termination of rainfall compared to outside, and the higher the rainfall level, the shorter the time lag of throughfall. For throughfall and stemflow, variations of time lag were (67.8 ± 7.8)--(17.2 ± 3.9) min and (112.0 ± 38.8)--(48.3 ± 10.6) min, respectively. The time lag of throughfall decreased with the increasing rainfall intensity under the same rainfall level. When the rainfall intensity was greater than 2 mm · h(-1), the time lag of throughfall was shortened significantly, but it increased with prolonging the antecedent dry period before rainfall. Rainfall would be the critical factor to affect the time lag of throughfall when the antecedent dry period was longer than 48 h. Termination of throughfall also lagged when rainfall termination happened with a rainfall greater than 5.0 mm. The time lag of throughfall termination increased with increasing the rainfall intensity, but it had no significant relationship with the antecedent dry period before rainfall. However, the termination of stemflow occurred prior to rainfall, which was relevant to the rainfall level, and the smaller the rainfall level, the sooner the stemflow terminated.

Florestas , Larix/fisiologia , Chuva , China
Exp Ther Med ; 1(2): 277-283, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22993540


To investigate the in vivo and in vitro inhibitory effects of deuterium-depleted water (DDW) on human lung cancer and the possible mechanisms underlying these effects, we cultured and treated human lung carcinoma cell line A549 and human embryonic lung fibroblasts HLF-1 with various concentrations of DDW from 2 to 72 h. Cellular growth inhibition rates were determined using the 3-(4, 5-dimethyldiazol-2-yl)-2, 5-diphenyltetrazolium-bromide) (MTT) proliferation assay. A549 cells were treated with 50±5 ppm DDW, and the morphology and structure of cells were observed by scanning electron microscopy (SEM). We observed alterations in the cellular skeleton by transmission electron microscopy (TEM) and changes in cell cycle by flow cytometry. Our data showed that DDW significantly inhibited the proliferation of A549 cells at a specific time point, and cells demonstrated the characteristic morphological changes of apoptosis under SEM and TEM. The length of the S phase increased significantly in cells treated with 50 ppm DDW, whereas the G0 to G1 phase and G2 to M phase were decreased. We observed DDW-induced cellular apoptosis using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DNA fragment analyses. In addition, we established a tumor transplantion model by injecting H460 tumor cells into subcutaneous tissue of BALB/c mice treated with DDW for 60 days. We determined the tumor inhibition rate of treated and control groups and found that the tumor weight was significantly decreased and the tumor inhibition rate was approximately 30% in the DDW group. We conclude that DDW is a promising new anticancer agent with potential for future clinical application.