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1.
Otolaryngol Head Neck Surg ; : 194599821989622, 2021 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-33618572

RESUMO

OBJECTIVE: This study summarizes the anatomical features of the superior laryngeal nerve in Chinese to enable the rapid location of the superior laryngeal nerve during an operation. STUDY DESIGN: Retrospective analysis of anatomical data. SETTING: Hangzhou First People's Hospital Affiliated to Nanjing Medical University. METHODS: A total of 71 embalmed human cadavers (132 heminecks) were examined over 3 months. The length and diameter of the internal and external branches of the superior laryngeal nerve and their relationships with different landmarks were recorded. RESULTS: The total length of the internal branch of the superior laryngeal nerve was 23.4 ± 6.9 mm. The length of the external branch of the superior laryngeal nerve was 47.7 ± 11.0 mm. Considering the midpoint of the lower edge of the thyroid cartilage as the starting point and using that edge as a horizontal line, when the entry point is above that line, the external branch of the superior laryngeal nerve can be found within 41.1 mm and at an angle of 57.2°. When the entry point is below the lower edge of the thyroid cartilage, the external branch of the superior laryngeal nerve can be found within 34.0 mm and at an angle of 36.5°. CONCLUSION: The superior laryngeal nerve in Chinese people has distinct anatomical characteristics. This article provides a new method of quickly locating the external branch of the superior laryngeal nerve during the operation, which can reduce the probability of damaging the external branch of the superior laryngeal nerve.

2.
Nat Commun ; 12(1): 1131, 2021 02 18.
Artigo em Inglês | MEDLINE | ID: mdl-33602900

RESUMO

Transcription activation of bacteriophage T4 late genes is accomplished by a transcription activation complex containing RNA polymerase (RNAP), the promoter specificity factor gp55, the coactivator gp33, and a universal component of cellular DNA replication, the sliding clamp gp45. Although genetic and biochemical studies have elucidated many aspects of T4 late gene transcription, no precise structure of the transcription machinery in the process is available. Here, we report the cryo-EM structures of a gp55-dependent RNAP-promoter open complex and an intact gp45-dependent transcription activation complex. The structures reveal the interactions between gp55 and the promoter DNA that mediate the recognition of T4 late promoters. In addition to the σR2 homology domain, gp55 has a helix-loop-helix motif that chaperons the template-strand single-stranded DNA of the transcription bubble. Gp33 contacts both RNAP and the upstream double-stranded DNA. Gp45 encircles the DNA and tethers RNAP to it, supporting the idea that gp45 switches the promoter search from three-dimensional diffusion mode to one-dimensional scanning mode.

3.
Crit Care Med ; 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33566467

RESUMO

OBJECTIVES: Although early recognition of sepsis is vital to improving outcomes, the diagnosis may be missed or delayed in many patients. Acute kidney injury is one of the most common organ failures in patients with sepsis but may not be apparent on presentation. Novel biomarkers for acute kidney injury might improve organ failure recognition and facilitate earlier sepsis care. DESIGN: Retrospective, international, Sapphire study. SETTING: Academic Medical Center. PATIENTS: Adults admitted to the ICU without evidence of acute kidney injury at time of enrollment. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: We stratified patients enrolled in the Sapphire study into three groups-those with a clinical diagnosis of sepsis (n = 216), those with infection without sepsis (n = 120), and those without infection (n = 387) at enrollment. We then examined 30-day mortality stratified by acute kidney injury within each group. Finally, we determined the operating characteristics for kidney stress markers (tissue inhibitor of metalloproteinases-2) × (insulin-like growth factor binding protein 7) for prediction of acute kidney injury as a sepsis-defining organ failure in patients with infection without a clinical diagnosis of sepsis at enrollment. Combining all groups, 30-day mortality was 23% for patients who developed stage 2-3 acute kidney injury within the first 3 days compared with 14% without stage 2-3 acute kidney injury. However, this difference was greatest in the infection without sepsis group (34% vs 11%; odds ratio, 4.09; 95% CI, 1.53-11.12; p = 0.005). Using a (tissue inhibitor of metalloproteinases-2) × (insulin-like growth factor binding protein 7) cutoff of 2.0 units, 14 patients (11.7%), in the infection/no sepsis group, tested positive of which 10 (71.4%) developed stage 2-3 acute kidney injury. The positive test result occurred a median of 19 hours (interquartile range, 0.8-34.0 hr) before acute kidney injury manifested by serum creatinine or urine output. Similar results were obtained using a cutoff of 1.0 for any stage of acute kidney injury. CONCLUSIONS: Use of the urinary (tissue inhibitor of metalloproteinases-2) × (insulin-like growth factor binding protein 7) test could identify acute kidney injury in patients with infection, possibly helping to detect sepsis, nearly a day before acute kidney injury is apparent by clinical criteria.

4.
Clin Immunol ; 225: 108683, 2021 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-33549832

RESUMO

Monocytes are a versatile and dynamic population, but its implication in Behçet's Disease (BD) remains elusive. We investigated the proportion, phenotypical and functional alterations in classical monocytes (CM), intermediate monocytes (IM) and non-classical monocytes (NCM) subsets in BD. A higher IM and lower NCM population in BD patients were noted, closely correlated with disease activity, and rescued after treatment. CD11b and CD64 expression on CM, IM and NCM in BD were enhanced. BD CM promoted TNF-a and IL-6 production and facilitated Th1 differentiation. BD IM promoted IL-6 production. We further demonstrated a higher level of phosphorylated p65 (p-p65) in BD CM and increased p-p65 and p-p38 in BD IM. Our data highlighted the aberrant populations of IM and CM in BD, potentially implicated in BD' pathogenesis.

5.
Sci Adv ; 7(4)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33523920

RESUMO

Annexin-A1 (ANXA1) has recently been proposed to play a role in microglial activation after brain ischemia, but the underlying mechanism remains poorly understood. Here, we demonstrated that ANXA1 is modified by SUMOylation, and SUMOylated ANXA1 could promote the beneficial phenotype polarization of microglia. Mechanistically, SUMOylated ANXA1 suppressed nuclear factor κB activation and the production of proinflammatory mediators. Further study revealed that SUMOylated ANXA1 targeted the IκB kinase (IKK) complex and selectively enhanced IKKα degradation. Simultaneously, we detected that SUMOylated ANXA1 facilitated the interaction between IKKα and NBR1 to promote IKKα degradation through selective autophagy. Further work revealed that the overexpression of SUMOylated ANXA1 in microglia/macrophages resulted in marked improvement in neurological function in a mouse model of cerebral ischemia. Collectively, our study demonstrates a previously unidentified mechanism whereby SUMOylated ANXA1 regulates microglial polarization and strongly indicates that up-regulation of ANXA1 SUMOylation in microglia may provide therapeutic benefits for cerebral ischemia.

6.
Appl Opt ; 60(2): 291-295, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-33448951

RESUMO

A large number of space activities are generating a high amount of undesirable space debris, which causes inevitable damage to spacecraft and satellites. Moreover, the damage assessment of ultrahigh-speed debris is a challenging task that requires both theoretical and ground-level experimental simulations. One should note that the location and damage degree can be preliminarily determined by measuring the impact flash spectrum, which provides basic data for damage assessment. Herein, the radiation spectrum of an ultrahigh-speed collision between plastic projectile and aluminum target is measured by using spectroscopic technology. The surface temperature of the colliding material, electron temperature, and electron density in the plasma are simultaneously retrieved by using a single-frame spectrum. The single-frame spectrum is separated into a continuous spectrum and a line spectrum by using continuous thermal radiation spectrum inversion material interface temperature and line spectrum inversion electron temperature and electron density in the plasma.

7.
Nanotechnology ; 32(15): 155705, 2021 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-33511964

RESUMO

Recently, graphene has led to unprecedented progress in device performance at the atom limit. A high performance of field-effect transistors requires a low graphene-metal contact resistance. However, the chemical doping methods used to tailor or improve the properties of graphene are sensitive to ambient conditions. Here, we fabricate a single-layer perfluorinated polymeric sulfonic acid (PFSA), also known as Nafion, between the graphene and the substrate as a p-type dopant. The PFSA doping method, without inducing any additional structural defects, reduces the contact resistance of graphene by ∼28.8%, which has a significant impact on practical applications. This reduction can be maintained for at least 67 days due to the extreme stability of PFSA. Effective, uniform and stable, the PFSA doping method provides an efficient way to reduce the contact resistance of graphene applications.

8.
Eur J Med Chem ; 213: 113174, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33515864

RESUMO

In order to discover and develop drug-like anti-inflammatory agents against arthritis, based on "Hit" we found earlier and to overcome drawbacks of toxicity, twelve series of total 89 novel pyrimidine, pyrazolo[4,3-d]pyrimidine and thieno[3,2-d]pyrimidine derivatives were designed, synthesized and screened for their anti-inflammatory activity against NO and toxicity for normal liver cells (LO2). Relationships of balance toxicity and activity have been summarized through multi-steps, and title compounds 22o, 22l were found to show lower toxicity (against LO2: IC50 = 2934, 2301 µM, respectively) and potent effect against NO release (IR = 98.3, 97.67%, at 10 µM, respectively). Furthermore, compound 22o showed potent iNOS inhibitory activity with value of IC50 is 0.96 µM and could interfere stability and formation of the active dimeric iNOS. It's anti-inflammatory activity in vivo was assessed by AIA rat model. Furthermore, the results of metabolic stability, CYP, PK study in vivo, acute toxicity study and subacute toxicity assessment indicated this compound had good drug-like properties for treatment.

9.
J Cell Biochem ; 122(3-4): 472-484, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33399232

RESUMO

HnRNPA2/B1 is highly expressed in many tumors. However, the role of hnRNPA2/B1 in breast cancer is not clear. In this study, we found the proliferation rate was decreased after knockout of hnRNPA2/B1 by CRISPR-CAS9 in MCF-7 cells, as demonstrated by the reduced expression of CDK4 and p-AKT, and the increased expression of P27. Besides this, the western blot results showed that knockout of hnRNPA2/B1 increased the rate of apoptosis and declined autophagy. By in vivo assay, we found that knockout of hnRNPA2/B1 suppressed tumor growth in a xenograft mouse model. Immunohistochemical staining results confirmed knockout of hnRNPA2/B1 impaired tumor angiogenesis, as illustrated by downregulated expression of VEGF-A. Besides this, interacting proteins with hnRNPA2/B1 were identified by mass spectrometry and the PPI network was constructed. GO analysis suggests that the Interacting proteins are mainly enriched in the Wnt signaling pathway, tumor necrosis factor-mediated signaling pathway, translation, and so on. We then identified hnRNPA2/B1 interacted with signal transducer and activator of transcription 3 (STAT3), as supported by the colocalization of hnRNPA2/B1 and STAT3. Meanwhile, knockout of hnRNPA2/B1 inhibited the phosphorylation of STAT3. Collectively, our results demonstrate that hnRNPA2/B1 promotes tumor cell growth in vitro and in vivo by activating the STAT3 pathway, regulating apoptosis and autophagy.

10.
Gene ; 775: 145448, 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33482281

RESUMO

PURPOSE: The expression level of aminopeptidase N (APN) is evidently correlated with porcine epidemic diarrhea virus (PEDV) infectivity. This study aims to examine the mechanisms regulating APN expression level in response to PEDV infection. METHODS: Quantitative real time PCR was performed herein to detect gene expression dynamics at various timepoints after PEDV infection. Subsequently, CRISPR/Cas9 gene editing technology was used to generate a APN-knockout IPEC-J2 cell line, exploring the effects of APN on cell proliferation by propidium iodide staining and anti-PEDV activity by indirect immunofluorescence assay. Ultimately, the effects of single nucleotide polymorphisms (SNPs) and methylation in the APN promoter region on gene expression were analyzed by using bisulfite sequencing PCR and dual luciferase reporter gene assay. RESULTS: APN expression was significantly upregulated within 4-24 h post-infection. The cytoactivity of the APN-knockout IPEC-J2 cell line was markedly suppressed at different timepoints. Further, cell cycle analyses indicated an increase in the number of G1-phase cells and a significant decrease in that of S-phase cells. Moreover, key cyclical factors regulating the G1 phase were highly expressed in APN-knockout cells. The RNA copies of viral particles and mRNA levels of antiviral genes and inflammatory cytokines in APN-knockout cells were markedly decreased within 24 h of PEDV infection. Similarly, indirect immunofluorescence assay confirmed that the number of PEDV particles was significantly decreased. Sequence analysis revealed two CpG islands in the APN promoter region. However, there was no evident correlation between the methylation status of APN promoter and mRNA levels. Dual luciferase reporter gene assay showed that the SNP rs326030589 (G/A) significantly increased the promoter activity of APN. CONCLUSIONS: These results suggested that APN knockout enhanced the resistance of IPEC-J2 cells to PEDV. Moreover, rs326030589 in the APN promoter region participated in gene transcription regulation. Our results provide a reference for studying the mechanisms regulating APN and may contribute to the application of APN gene in resistance breeding of swine epidemic diarrhea.


Assuntos
Antígenos CD13/genética , Resistência à Doença , Polimorfismo de Nucleotídeo Único , Vírus da Diarreia Epidêmica Suína/patogenicidade , Regulação para Cima , Animais , Antígenos CD13/metabolismo , Sistemas CRISPR-Cas , Ciclo Celular , Linhagem Celular , Proliferação de Células , Metilação de DNA , Técnicas de Inativação de Genes , Vírus da Diarreia Epidêmica Suína/genética , Regiões Promotoras Genéticas , RNA Viral/genética , Suínos
11.
Biomaterials ; 269: 120634, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33421708

RESUMO

Light-triggered therapy is a prospective method to combat implant-associated infection but near-infrared I (NIR-I) light has insufficient penetrating ability in tissues and local hyperthermia induced by the photothermal treatment may destroy surrounding healthy tissues. Herein, a near-infrared II (NIR-II) phototherapy system composed of upconversion elements doped titanium dioxide nanorods (TiO2 NRs)/curcumin (Cur)/hyaluronic acid (HA)/bone morphogenetic protein-2 (BMP-2) is designed for biomedical titanium and demonstrated to overcome the above hurdles simultaneously. Incorporation of F, Yb, and Ho not only improves the photocatalytic ability, but also renders the implants with the upconversion capability, so that the NRs can generate enough reactive oxygen species (ROS) when irradiated by the NIR-II laser. Furthermore, the combined actions of quorum sensing inhibitors, ROS, and physical puncture by NRs eliminate Staphylococcus aureus biofilms on titanium rapidly at a mild temperature of 45 °C by only requiring irradiation with the 1060 nm laser for only 15 min in vitro and in vivo. The presence of Cur mitigates the immune response and BMP-2 improves osteogenic differentiation, thus accelerating new bone formation. This low-temperature NIR-II light-triggered antibacterial platform has large potential in combating deep-tissue infection in clinical applications.

12.
Ann Palliat Med ; 10(1): 10-15, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33440959

RESUMO

BACKGROUND: Gefitinib is a first-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI). It was approved by the U.S. Food and Drug Administration (FDA) for clinical use in 2003. However, gefitinib has only come to China in recent years. Previous studies have not compared the efficacy and safety of domestic and imported gefitinib. Therefore, we conducted this study. METHODS: This study included 227 patients with advanced non-small cell lung cancer (NSCLC) who received gefitinib treatment in four medical institutions: The First Affiliated Hospital of USTC, Division of life Sciences and Medicine, University of Science and Technology of China, Anhui Provincial Cancer Hospital, Fudan University Shanghai Cancer Center, Shandong Provincial Institute of Cancer Prevention and Jiangsu Cancer Hospital, from January 2017 to July 2018. The patients were divided into a Yiruike group (55 patients treated with domestic gefitinib, Yiruike) and an Iressa group (172 patients treated with imported gefitinib, Iressa). Because gefitinib resistance usually occurs within 8-10 months of gefitinib administration, the patients were followed up for one year to observe their conditions and compare the occurrence of adverse reactions between the two groups. RESULTS: The two groups had no significant difference in baseline data. The median progression-free survival (PFS) of Yiruike group and that of Iressa group were 10.270±2.036 and 12.970±1.634 months, respectively. The mean PFS of Yiruike group and that of Iressa group were 12.598±1.083 and 15.958±0.987 months, respectively. The one-year disease control rate (DCR) of Yiruike group and that of Iressa group were 61.8% and 59.3%, respectively. The differences were all insignificant (P>0.05). The incidence of adverse reactions in these two groups were not significantly different. CONCLUSIONS: Yiruike was slightly superior to Iressa in terms of DCR. However, comparisons of bioequivalence and DCR were not sufficient for evaluating a drug. Other comparisons require long-term follow-up studies with a large sample size.

13.
Life Sci ; 267: 118986, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33385408

RESUMO

AIMS: Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease and lacks for safe and effective drug to therapy completely. Ginsenoside-Rg1 is one of the main components of ginseng and has been proved to counteract a variety of diseases. However, there is currently a lack of sufficient evidence to support the efficacy of ginsenoside-Rg1 in the treatment of NAFLD. Our aim was to investigate whether Ginsenoside-Rg1 is a potential drug for NAFLD. MAIN METHODS: NAFLD model in rats was established by giving a high-fat diet (HFD), ginsenoside-Rg1 was intragastrically administered 100 mg/kg/d for 8 weeks in NAFLD rat. Serum biochemical indices were measured. Liver tissues were stained with hematoxylin and eosin (HE) and oil red O. Total RNA was extracted from liver and was used for high throughput sequencing to identify the changes of transcriptome. The relevant hub genes were verified by quantitative real-time PCR and western blot. KEY FINDINGS: Serum biochemical analysis indicated that ginsenoside-Rg1 improved liver function. Additionally, the staining of HE and oil red O indicated ginsenoside-Rg1 could remit pathology process of NAFLD. The transcriptome changes also support this result and reveals Atf3 and Acox2 were key genes. SIGNIFICANCE: Taken together, these results suggest that the efficiency of ginsenoside-Rg1 against NAFLD and confirmed that ginsenoside-Rg1 is a potential effective drug in treatment of NAFLD.

14.
Nanoscale ; 2021 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-33475665

RESUMO

The development of carbon materials for potassium storage is limited by their low specific capacity and poor cycling stability due to the sluggish kinetics of K ions. Herein, fucoidan-derived oxygen-rich carbon nanosheets are reported as a fantastic anode for potassium ion batteries. Attributed to its 2D porous sheet-like structure (morphology engineering), rich oxygen doping (defect engineering), and dilated graphitic layer in an amorphous structure (structure engineering), a competitive capacity of 392 mA h g-1 at 0.05 A g-1 and a long cycling span over 2500 cycles at 2 A g-1 was achieved for the carbon anode, outperforming most of the reported carbons. The kinetic analyses reveal that rich active sites and a porous nanosheet structure account for the superb rate performance and cycling stability of the material. Ex situ X-ray photoelectron spectroscopy measurements demonstrate that the introduction of C[double bond, length as m-dash]O greatly promotes K+ adsorption, and that the improvement of the C[double bond, length as m-dash]O bonds during cycling contributes to enhancement in the capacity. The fabricated potassium ion hybrid capacitor displays an exceptional energy/power density of 193 W h kg-1/22 324 W kg-1, and a promising cycling stability with 99.3% capacity retention over 2000 cycles. This work provides a large-scale synthesis strategy for preparing oxygen-rich carbon nanosheets for advanced potassium ion storage.

15.
J Nucl Cardiol ; 2021 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-33462788

RESUMO

BACKGROUND: Considering the few reported cardiac masses, PET/CT in the imaging workup of cardiac masses is not well established. This retrospective study analyzed the role of 18F-FDG PET/CT imaging in cardiac/pericardial masses. METHODS AND RESULTS: Fifty-nine patients with newly diagnosed cardiac/pericardial masses who underwent PET/CT and transthoracic echocardiography (TTE) were recruited. Echocardiographic and PET/CT characteristics were evaluated for predictive value in differentiating malignant and non-malignant lesions using histologic confirmation as the gold standard. The McNemar test was used to test the differences in sensitivity between PET/CT and TTE. 18F-FDG PET/CT had higher sensitivity in determining the malignancy of cardiac/pericardial masses compared to TTE (sensitivity, 96.6% vs 72.4%, P = .039). However, when pericardial masses were excluded from the analysis, the difference in sensitivity between the two was not statistically significant (sensitivity, 95.6% vs 78.3%, P = .219). 18F-FDG PET/CT identified two malignant pericardial masses missed on TTE, changed the diagnostic orientation of TTE in 15 patients, and found seven patients with extracardiac lesions in 29 malignant patients. CONCLUSIONS: PET/CT was an effective additional image modality in patients with suspected malignant cardiac mass for further confirmation and to screen for potential metastasis.

16.
J Gambl Stud ; 2021 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-33486640

RESUMO

Voluntary self-exclusion programs allow gamblers to voluntarily be denied access to gambling venues for an agreed upon period. Many people who self-exclude decide to return to gambling venues after the exclusion period has ended, however people who reinstate may be at risk for the recurrence of gambling problems. This study was designed to determine the efficacy of a tutorial created with the intent of reducing the risk of harm to those who reinstate. People who wished to be reinstated were asked to complete a survey on gambling related issues and then watch the tutorial video. An online video-based tutorial designed to reduce gambling related harm and to provide information about treatment services was developed. The control group (N = 131) consisted of people who reinstated in the year prior to the implementation of the online tutorial. The experimental intervention group (N = 104) were those who reinstated after the implementation of the online tutorial. There was a significant decrease in gambling and problem gambling comparing pre-exclusion to during exclusion in both the experimental and control group. Furthermore, this drop in gambling problem was sustained for 6-months and 12-months after reinstatement. However, no main effect or interaction was found that supported the efficacy of the tutorial. Self-exclusion by itself was associated with a sustained reduction in problem gambling. There was no significant evidence that the educational tutorial had any additional impact on the reinstatement process.

17.
Hum Exp Toxicol ; : 960327120984225, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33401961

RESUMO

Psoriasis is a common immune-mediated and genetic skin disease. Forkhead box M1 (FOXM1) is a member of FOX family that has been found to modulate skin disorders. However, its role in psoriasis remains unknown. Thus, we aimed to investigate the effect of FOXM1 on keratinocytes in response to tumor necrosis factor-α (TNF-α). The expression levels of FOXM1 in psoriasis tissues and normal skin tissues were examined using qRT-PCR and western blot. HaCaT cells were stimulated by TNF-α to mimic psoriasis in vitro. MTT assay was performed to assess cell proliferation. The caspase-3 activity and expression levels of bcl-2 and bax were determined to indicate cell apoptosis. The mRNA and secretion levels of IL-6, IL-23 and TGF-ß were determined by qRT-PCR and ELISA, respectively. The NF-κB activation was assessed using western blot analysis. Our results demonstrated that FOXM1 was highly upregulated in psoriatic skin tissues and TNF-α-stimulated HaCaT cells. Knockdown of FOXM1 repressed cell proliferation of TNF-α-stimulated HaCaT cells. Knockdown of FOXM1 caused significant increases in caspase-3 activity, bax expression and decrease in bcl-2 expression in TNF-α-stimulated HaCaT cells. Moreover, FOXM1 knockdown also suppressed the TNF-α-induced production of IL-6, IL-23, and TGF-ß in HaCaT cells. However, FOXM1 overexpression showed the opposite effect. Furthermore, the TNF-α-induced NF-κB activation was prevented by FOXM1 knockdown. Additionally, inhibition of NF-κB reversed the effects of FOXM1 on HaCaT cells. Taken together, these findings indicated that FOXM1 regulated cell proliferation, apoptosis and inflammation in TNF-α-induced HaCaT cells. The effects of FOXM1 were mediated by NF-κB pathway.

18.
Sci Total Environ ; 760: 143949, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33340737

RESUMO

Using diffusive gradients in thin films (DGT) is a recently developed alternative method of rapidly evaluating the bioavailability of metals in soil. However, the method has found only limited application in systematic assessment of the bioavailability of cadmium (Cd) in red limestone paddy soils treated with different soil amendments. Of the four methods compared for estimating Cd content of rice grains from plants grown in such soils of central China treated with eleven different soil amendments in pot culture, Cd content of DGT-labile soil was significantly correlated to Cd concentrations in brown rice (R = 0.447, p < 0.01). The other three methods involved CaCl2, diethylenetriaminepentaacetic acid (DTPA), or NH4NO3. Some other properties of soil, such as pH, redox potential, content of dissolved organic matter, and cation exchange capacity were also determined. A simple algorithm developed to evaluate the sensitivity of the four methods also confirmed DGT as the most efficient method to predict the bioavailability of Cd in red limestone paddy soils.


Assuntos
Oryza , Poluentes do Solo , Disponibilidade Biológica , Cádmio/análise , China , Solo , Poluentes do Solo/análise
19.
Huan Jing Ke Xue ; 42(1): 9-18, 2021 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-33372452

RESUMO

Pollution occurs in the boundary layer, and the thermal and dynamic vertical structure of the boundary layer has a significant influence on the formation of heavy pollution episodes. Based on unmanned aerial vehicle (UAV) sounding, ground-based remote sensing and numerical modeling, this paper analyzes the vertical structure of the boundary layer and the causes of pollution during the heavy pollution episode in Tianjin from January 10 to 15, 2019, with a view to strengthening the understanding of the influence law of boundary layer processes on heavy pollution in northern coastal cities and improving the accuracy of weather forecasts and heavy pollution warnings. The results show that atmospheric temperature stratification had a significant influence on the formation, persistence, and dissipation of heavy pollution episodes. During an episode, accompanied by the development and dissipation of the inversion layer, a high PM2.5 concentration area developed to the upper atmosphere with a height of over 300 m in the daytime and compressed to the ground at night with a height about 100 m. When fog appeared and continued in the daytime, the vertical structure characteristics of the boundary layer changed. A temperature inversion above the fog restrained the diffusion of pollutants to the upper air and made the contribution of turbulence vertical mixing process decrease significantly in the daytime, leading to the persistence and development of heavy pollution near the surface. Regional pollution transport accounted for 66.6% during the episode, which was closely related to regional pollution transport. Regional pollution transport mainly appeared at the top of the boundary layer and above the fog inversion layer where high wind speeds occurred. Pollutants were transported to the ground by a sinking motion as the boundary layer and fog height changed. This is how regional pollution transport occurred when Tianjin was controlled by a weak high pressure field in the north. The vertical structure of the boundary layer also affected the improvement of air quality by cold air. The strong temperature inversion at the top of the fog resulted in the failure of the cold air to transmit to the ground through turbulent shear stress in the S3 stage. There was an obvious difference in wind speed between the upper and lower air. The influence of cold air on the ground was delayed, and the effect of it was weakened. Thus, the heavy pollution episode could not be alleviated completely.

20.
Mater Sci Eng C Mater Biol Appl ; 118: 111447, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33255036

RESUMO

The numerous advantages of hydrogel make it possible to apply as dressing. However, it is challenging in designing hydrogels with desired antibacterial activity and enhanced mechanical properties at the same time. Herein, a graphene oxide/rose bengal/polyvinyl alcohol hybrid hydrogel (ß-GO/RB/PVA HD) is prepared by freezing and thawing a mixed polyvinyl alcohol (PVA) solution of rose bengal (RB) immobilized with chitosan microspheres (CM) and a modified graphene oxide network (ß-GO). The mechanical properties and light-triggered antibacterial activity of hydrogel are systematically evaluated. The ß-GO inorganic network interpenetrate into the PVA porous structure, which significantly improves the mechanical properties of hydrogel. The hyperthermia generated by ß-GO under 808 nm light irradiation combined with reactive oxygen species (ROS) produced by RB under 550 nm light irradiation give rise to excellent antibacterial activity requiring irradiation for only 10 min as demonstrated by our experiments conducted in vitro and in vivo. Meanwhile, ß-GO/RB/PVA HD exhibits outstanding biocompatibility and water-absorbing capacity. More importantly, the hybrid hydrogel can significantly accelerate bacteria-accompanied wound healing. The results demonstrated that the hybrid hydrogel could be a promising wound dressing for preventing bacterial infection.

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