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1.
J Hazard Mater ; 401: 123382, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32763685

RESUMO

Pinewood biochar (PBC)-supported metallic silver (Ago) was prepared via a one-step carbothermal reduction route (AgH) or a wet-chemistry reduction method (AgW). XRD and SEM confirmed Ago was soldered on PBC matrix. Low methylene blue (MB) sorption was observed for unsupported Ago nanoparticles (AgNP), AgH and AgW. Under ultraviolet (UV) light irradiation, net MB degradation by AgH (15.88 g kg-1) was higher than that of AgW (12.50 g kg-1) and AgNP (10.27 g kg-1). TOC removal percentages after degradation corresponded largely to reduction of MB concentrations in solution, indicating MB was dominantly mineralized. Electron paramagnetic resonance (EPR) revealed that MB was degraded by reactive oxygen species (ROS) such as hydroxyl radical (OH), superoxide radical (O2-) and singlet oxygen (1O2). The scavenging experiments further suggested that OH scavengers suppressed MB degradation to a greater extent than other quenchers. Compared to AgW, AgH possessed greater abundance of persistent free radicals, which enhance ROS generation. PBC could also improve separation of electron-hole (e--h+) pairs and enhance electron transfer ascribing to favorable carbon structure. Besides, PBC-Ago maintained good antimicrobial efficacy over E.coli DH5α. This work presented a facile carbothermal route to prepare Ago-based photocatalysts for dye removal and microbial inhibition in industrial wastewater.

2.
J Ethnopharmacol ; 265: 113345, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32890713

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Periploca sepium Bunge (P. sepium) is used in traditional Chinese medicine (TCM) for the treatment of autoimmune diseases, particularly rheumatoid arthritis. Periploca sepium periplosides (PePs), isolated from the root bark of P. sepium, characterized as the cardiac glycosides-free pregnane glycosides fraction, is expected to possess therapeutic potential on inflammatory arthritis. AIM OF THE STUDY: The current study is designed to evaluate the anti-nociceptive, anti-inflammatory and anti-arthritic activities effects of the PePs. MATERIALS AND METHODS: The anti-nociceptive activity of PePs was examined in the writhing test and hot-plate test in mice. The anti-inflammatory activity of PePs was determined by the 2, 4-dinitro-1-fluorobenzene (DNFB)-induced ear edema model and the carrageenan induced paw edema model in mice. The anti-arthritic activity of PePs was investigated by evaluating the joint inflammation and arthritis pathology in rat adjuvant induced arthritis (AIA) and murine collagen induced arthritis (CIA). Phytohaemagglutinin M (PHA-M) -elicited human peripheral blood mononuclear cells (PBMCs) were further applied to assess the suppressive activity of PePs on IFN-γ and IL-17 production. RESULTS: PePs treatment markedly decreased the acetic acid-induced visceral nociceptive response and increased the hot-plate pain threshold. Further, oral administration of PePs exhibited anti-inflammatory activity by decreasing DNFB-induced ear edema in mice and carrageenan-induced paw edema in rats. Moreover, oral treatment of PePs ameliorated joint swelling and attenuated bone erosion in rodent arthritis, and the therapeutic benefits were partially attributed to the suppression of proinflammatory cytokines such IFN-γ and IL-17. Moreover, PePs suppressed the proliferation as well as IFN-γ and IL-17 secretion in PHA-M-elicited human PBMCs in a concentration dependent manner. CONCLUSIONS: Taken together, our results justified the traditional use of Periploca sepium Bunge for the treatment of diseases associated with inflammation and pain.

3.
Mol Med Rep ; 23(1)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33236143

RESUMO

Macrophage­capping protein (CapG) is a newly characterized oncogene involved in several types of cancer. However, the expression patterns and biological mechanisms of CapG in clear cell renal cell carcinoma (ccRCC) are unclear. The present study aimed to investigate the roles of CapG in the prognosis, proliferation and metastasis of ccRCC. In the present study, the expression of CapG was analyzed by western blotting in 24 paired ccRCC and adjacent normal tissue samples. Another 152 tissue samples from 152 patients with ccRCC were examined by immunohistochemistry. Compared with normal tissue, CapG expression was significantly increased in ccRCC tissue, and high CapG expression was associated with advanced tumor stage, histological grade, lymph node metastasis, and poor overall survival. Moreover, CapG was an independent predictor of survival. Lentivirus­mediated CapG knockdown significantly inhibited 786­O cell proliferation, migration, and invasion, induced cell cycle arrest at the G2/M phase, and increased apoptosis in vitro. Microarray analysis indicated that RAC, CDC42 and ERK/MAPK signaling were disrupted by CapG knockdown in 786­O cells. In conclusion, the present findings indicate that CapG plays an oncogenic role in ccRCC and may represent a potential therapeutic target for this disease.

4.
Phys Chem Chem Phys ; 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33165454

RESUMO

The electrochemical reduction of N2 is a promising reaction candidate for the ammonia synthesis process. Density functional theory simulations are carried out to study the reaction thermodynamics and kinetics for a better understanding of the catalytic performance of Fe, Mo, Rh, and Ru electrodes. The distal pathway is the most likely reaction pathway for nitrogen reduction on transition metal surfaces according to the computed reaction free energies. The onset potential of nitrogen reduction on Fe(110) (-0.49 V) and Mo(110) (-0.52 V) is determined by the hydrogenation of NH to NH2, which is more positive than the onset potential on the Ru(0001) (-0.76 V) and Rh(111) (-0.98 V) surfaces attributed to the hydrogenation of N2 to NNH. In particular, the initial hydrogenation of N2 on Mo(111) is a spontaneous process due to the strong interaction of N2 and NNH with the Mo(110) surface. Electronic structure analyses including Bader charge analysis and projected crystal orbital Hamilton populations are performed to interpret the difference in adsorption energy of key intermediates on the four metal surfaces. It is found that both N2 and NNH species have the strongest interaction with Mo(110) leading to the initial activation of N2 on the Mo(110) surface being a spontaneous process. A kinetic model based on the Marcus theory is applied to calculate the potential-dependent reaction barrier of electrochemical hydrogenation steps of the N2 reduction reaction. The rate-determining step is the fifth hydrogenation step *NH → *NH2 on Fe(110) and Mo(110) surfaces, and the first hydrogenation step *N2 → *NNH on Rh(111) and Ru(0001) surfaces. The predicted electrocatalytic activity from the potential-dependent rate constant of the rate-determining step on the four metal electrodes decreases in sequence: Fe(110) > Mo(110) > Ru(0001) > Rh(111).

5.
Eur Radiol ; 2020 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-33219848

RESUMO

OBJECTIVE: To explore the natural history of pulmonary subsolid nodules (SSNs) with different pathological types by deep learning-assisted nodule segmentation. METHODS: Between June 2012 and June 2019, 95 resected SSNs with preoperative long-term follow-up were enrolled in this retrospective study. SSN detection and segmentation were performed on preoperative follow-up CTs using the deep learning-based Dr. Wise system. SSNs were categorized into invasive adenocarcinoma (IAC, n = 47) and non-IAC (n = 48) groups; according to the interval change during the preoperative follow-up, SSNs were divided into growth (n = 68), nongrowth (n = 22), and new emergence (n = 5) groups. We analyzed the cumulative percentages and pattern of SSN growth and identified significant factors for IAC diagnosis and SSN growth. RESULTS: The mean preoperative follow-up was 42.1 ± 17.0 months. More SSNs showed growth or new emergence in the IAC than in the non-IAC group (89.4% vs. 64.6%, p = 0.009). Volume doubling time was non-significantly shorter for IACs than for non-IACs (1436.0 ± 1188.2 vs. 2087.5 ± 1799.7 days, p = 0.077). Median mass doubling time was significantly shorter for IACs than for non-IACs (821.7 vs. 1944.1 days, p = 0.001). Lobulated sign (p = 0.002) and SSN mass (p = 0.004) were significant factors for differentiating IACs. IACs showed significantly higher cumulative growth percentages than non-IACs in the first 70 months of follow-up. The growth pattern of SSNs may conform to the exponential model. The initial volume (p = 0.042) was a predictor for SSN growth. CONCLUSIONS: IACs appearing as SSNs showed an indolent course. The mean growth rate was larger for IACs than for non-IACs. SSNs with larger initial volume are more likely to grow. KEY POINTS: • Invasive adenocarcinomas (IACs) appearing as subsolid nodules (SSNs), with a mean volume doubling time (VDT) of 1436.0 ± 1188.2 days and median mass doubling time (MDT) of 821.7 days, showed an indolent course. • The VDT was shorter for IACs than for non-IACs (1436.0 ± 1188.2 vs. 2087.5 ± 1799.7 days), but the difference was not significant (p = 0.077). The median MDT was significantly shorter for IACs than for non-IACs (821.7 vs. 1944.1 days, p = 0.001). • SSNs with lobulated sign and larger mass (> 390.5 mg) may very likely be IACs. SSNs with larger initial volume are more likely to grow.

6.
Comput Methods Programs Biomed ; 198: 105807, 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-33130497

RESUMO

BACKGROUND AND OBJECTIVE: Cervical cell classification has important clinical significance in cervical cancer screening at early stages. In contrast with the conventional classification methods which depend on hand-crafted or engineered features, Convolutional Neural Network (CNN) generally classifies cervical cells via learned deep features. However, the latent correlations of images may be ignored during CNN feature learning and thus influence the representation ability of CNN features. METHODS: We propose a novel cervical cell classification method based on Graph Convolutional Network (GCN). It aims to explore the potential relationship of cervical cell images for improving the classification performance. The CNN features of all the cervical cell images are firstly clustered and the intrinsic relationships of images can be preliminarily revealed through the clustering. To further capture the underlying correlations existed among clusters, a graph structure is constructed. GCN is then applied to propagate the node dependencies and thus yield the relation-aware feature representation. The GCN features are finally incorporated to enhance the discriminative ability of CNN features. RESULTS: Experiments on the public cervical cell image dataset SIPaKMeD from International Conference on Image Processing in 2018 demonstrate the feasibility and effectiveness of the proposed method. In addition, we introduce a large-scale Motic liquid-based cytology image dataset which provides the large amount of data, some novel cell types with important clinical significance and staining difference and thus presents a great challenge for cervical cell classification. We evaluate the proposed method under two conditions of the consistent staining and different staining. Experimental results show our method outperforms the existing state-of-arts methods according to the quantitative metrics (i.e. accuracy, sensitivity, specificity, F-measure and confusion matrices). CONCLUSIONS: The intrinsic relationship exploration of cervical cells contributes significant improvements to the cervical cell classification. The relation-aware features generated by GCN effectively strengthens the representational power of CNN features. The proposed method can achieve the better classification performance and also can be potentially used in automatic screening system of cervical cytology.

7.
J Pharm Biomed Anal ; 193: 113743, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33221573

RESUMO

In this study, a method based on adsorbed hollow fiber immobilized tyrosinase (TYR) was developed to screening potential TYR inhibitors from Pueraria lobate extract. Kojic acid and ranitidine were used as positive and negative control to verify the reliability of the proposed method, respectively. Several significant parameters of the screening process, including the amount of P. lobata extract, adsorption time and incubation time, were optimized. After investigating the repeatability of the developed method, seven potential active compounds in P. lobata extract were successfully detected and their chemical structures were tentatively identified by liquid chromatography - mass spectrometry analysis. Furthermore, the inhibitory activity of four identified compounds on TYR was tested in vitro, and three of them, namely, puerarin, puerarin-6″-O-xyloside and puerarin apioside were verified to have good TYR inhibitory activity with IC50 value of 478.5, 513.8, and 877.3 µM, respectively. In addition, the molecular docking results indicated that these compounds could bind to the amino acid residues in TYR catalytic pocket. These results proved that the proposed method is a feasible approach for screening of TYR inhibitors from plant extract.

8.
Haemophilia ; 2020 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-33245829

RESUMO

INTRODUCTION: Haemophilia A (HA) is a rare X chromosome-linked bleeding disorder resulting in missing or defective clotting factor VIII (FVIII) and causes large disease burden. AIM: As a member of World Federation of Hemophilia, China seeks to understand the current epidemiology, disease profile and treatment landscape of patients with HA through the Hemophilia Treatment Center Collaboration Network of China (HTCCNC). METHODS: The HTCCNC enabled data collection on patients with HA from 166 member hospitals (2007-2019) across China. The distribution of patients across 31 divisions was summarized using a heat map. Patient demographics, disease severity and clinical and treatment information were summarized using descriptive statistics. RESULTS: HTCCNC identified 17,779 patients with HA during 2007-2019. Patients were predominantly male (99.99%), and 28.3% had a known family history of haemophilia. Among patients with lab-measured disease severity (N = 13,116), 6,519 had severe HA (49.7%), 4,788 had moderate HA (36.5%), and 1,809 had mild HA (13.8%). Among patients with information on the delays, delays in diagnosis and in treatment initiation were observed in 1,437 (28.8%) and 1,750 (39.2%) patients, respectively. On average, those patients had an 8.4 years gap between the first bleed and HA diagnosis and a delay of 8.6 years from the first bleed to treatment initiation. Additionally, 44.33% of patients relied solely on episodic treatments, and 16.2% received any prophylaxis treatments. CONCLUSIONS: Using data from the largest haemophilia registry in China, this study indicated that delayed diagnosis and treatment, together with low utilization of prophylaxis, are key challenges for patients with HA.

9.
J Pharm Biomed Anal ; 192: 113675, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33099113

RESUMO

Sample preparation such as isolation and pre-concentration is a crucial step for the phytochemical analysis. Magnetic solid-phase extraction (MSPE) has received considerable attention, mainly due to its phase separation more conveniently by facile magnetic decantation as compared to traditional SPE. This review focused on the recent applications of MSPE in sample preparation for the analysis of phytochemical compounds in plants, biological samples and Chinese herbal preparations. In addition, the enzymes immobilized on the magnetic materials and used for the biospecific extraction of enzyme inhibitors were also discussed. The information summarized in this article may provide a reference to the further applications of MSPE in phytochemical analysis.

10.
Int J Mol Med ; 46(6): 2161-2171, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33125105

RESUMO

Renal tubular epithelial cells (RTEC) injury induced by hyperglycemia is considered a major contributor to the pathogenesis of diabetic nephropathy (DN). However, few studies have focused on the role of microRNAs (miRNAs/miRs) in RTEC injury. Therefore, the present study aimed to investigate the role and mechanisms of miRNAs in RTEC injury. In the study, miRNAs expression profiles were determined via microarray assay in the peripheral blood samples of patients with DN. High glucose (HG)­induced injury in HK­2 cells was used as a cell model to examine the potential role of miR­199a­3p in DN. The expression of miR­199a­3p was validated using reverse transcription­quantitative PCR. The expressions of TNF­α, IL­1ß and IL­6, were detected via ELISA. The protein levels of apoptosis­related proteins were determined using western blotting. Cell apoptosis and caspase 3 activity were evaluated via flow cytometry analysis and caspase 3 activity assay, respectively. Luciferase reporter assay was used to confirm the interaction between miR­199a­3p and IKKß. miR­199a­3p was found to be significantly downregulated in the peripheral blood samples, and there was a negative correlation between miR­199a­3p expression and proteinuria in patients with DN. It was identified that miR­199a­3p expression was time­dependently decreased in the HG­induced cell damage model. Moreover, miR­199a­3p overexpression significantly improved HG­induced cell injury, as evidenced by the decrease in cell apoptosis and inflammation. Subsequent analyses demonstrated that miR­199a­3p directly targeted IKKß, whose expression was increased, and negatively correlated with miR­199a­3p expression in patients with DN. The protective effects of miR­199a­3p overexpression on HG­treated HK­2 cells were partially reversed by IKKß overexpression. In addition, activation of the NF­κB pathway by HG was blocked by miR­199a­3p mimics transfection in HK­2 cells. Collectively, the present findings indicated that miR­199a­3p protected HK­2 cells against HG­induced injury via inactivation of the IKKß/NF­κB pathway, suggesting enhanced expression of miR­199a­3p as a potential therapeutic strategy for patients with DN.

11.
Medicine (Baltimore) ; 99(44): e22246, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33126297

RESUMO

In this study, micro-CT was used to observe the microscopic anatomy of the hyoid bone, examine the variation of the trabecular bone inside the hyoid bone, and investigate the internal structure of the hyoid bone.A total of 22 hyoid bones were scanned using micro-CT. The changes in the internal bone trabeculae were assessed with 3D reconstructions, and the fine anatomical structure of the hyoid bone was further analyzed.Micro-CT images showed the microstructure of various parts of the hyoid bone. There were significant differences in total volume, bone volume, bone area, bone density, and volume fraction between the body and greater horns of the hyoid bone (P < .05), but no significant differences in the ratio of bone area/volume and bone surface density were found between the body and greater horns of the hyoid bone (P > .05). In addition, significant differences in the trabecular bone measurements, bone trabecular connectivity, and Euler number were found between the body and greater horns of the hyoid bone (P < .05). Other parameters, including bone trabecular thickness, number of trabecular bones, bone trabecular structure model index, and anisotropy of bone trabeculae, did not differ between the body and greater horns of the hyoid bone (P > .05). There was noticeably ossified healing at the joint between the body and greater horns of the hyoid bone.Micro-CT can adequately display the internal structure of the hyoid bone. The identified bone structure may help clarify the physiological function of the hyoid bone. The present findings provide a theoretical basis for further studies aimed at pathological changes due to hyoid injury in clinical and forensic medicine.


Assuntos
Osso Hioide/ultraestrutura , Microtomografia por Raio-X , Anisotropia , Densidade Óssea , Humanos , Osso Hioide/diagnóstico por imagem , Imageamento Tridimensional
12.
Dent Mater J ; 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028786

RESUMO

In this study, we proposed BMP2-incorporated calcium phosphate cement (BMP2-CPC), for application in guided bone regeneration (GBR) and compared the experimental bone restoration performance and clinical alveolar bone reconstruction outcome of BMP2-CPC with those of deproteinized bovine bone (DBB). The animal study indicated that, compared to DBB, which induced the slow ingrowth of new bone, BMP2-CPC induced numerous small growth centers for bone regeneration and facilitated a significant amount of bone regeneration in rabbit calvarial bone defects. Fewer residual graft particles remained in the BMP2-CPC-treated defects than in the DBB-treated defects. The clinical study indicated that BMP2-CPC was similar to DBB in remedying alveolar bone insufficiency and maintaining implant stability. In conclusion, the results of this present study indicate that compared to DBB, BMP2-CPC can significantly enhance in vivo bone regeneration and remodeling in rabbit calvarial bone defects and shows preliminary support on its clinical application in GBR surgeries.

13.
Br J Clin Pharmacol ; 2020 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33119136

RESUMO

There is an urgent need for targeted and effective COVID-19 treatments. A number of medications, including hydroxychloroquine, remdesivir, lopinavir-ritonavir, favipiravir, tocilizumab and others have been identified as potential treatments for COVID-19. Bringing these repurposed medications to the public for COVID-19 requires robust and high-quality clinical trials that must be conducted under extremely challenging pandemic conditions. This article reviews translational science principles and strategies for conducting clinical trials in a pandemic and evaluates recent trials for different drug candidates. We hope that this knowledge will help focus efforts during this crisis and lead to the expedited development and approval of COVID-19 therapies.

14.
Phytother Res ; 2020 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-33111362

RESUMO

Ligustilide is a phenolic compound isolated from Asian plants of Umbelliferae family. This study was aimed at exploring the neuroprotective effects of Ligustilide from the perspective of endoplasmic reticulum stress (ERS) and autophagy. The Alzheimer's disease (AD) cell models were constructed by SH-SY5Y cell line, which was exposed to 20 µM Aß25-35 . CCK-8 was used to evaluate the cell viability of Ligustilide on AD cell model. Hoechst staining and LysoTracker Red were used to test the cell apoptosis and Lysosome function, respectively. ERS in living cells were detected by Thioflavin T. The expression of autophagy-related proteins (LC3B-II/I, P62/SQSTM1, Beclin1, and Atg5), ERS marker proteins (PERK, GRP78, and CHOH), and apoptosis proteins (Bax, Bcl-2, and Caspase-12) were analyzed by Western blot analyses. Aß25-35 could induce ERS and autophagy in a time-dependent manner in SH-SY5Y cells. We demonstrated that Ligustilide significantly decreased the rate of apoptosis, and improved the viability of cells. Simultaneously, Ligustilide effectively modulated ERS via inhibiting the over-activation of GRP78/PERK/CHOP signaling pathway. In addition, Ligustilide alleviated the accumulation of autophagy vacuoles, reduced the ratio of LC3B-II/I and the level of P62/SQSTM1. Ligustilide significantly up-regulated lysosomal acidity and the expression of Cathepsin D (CTSD). Ligustilide could rescue lysosomal function to promote autophagy flux and inhibit the over-activation of ERS. This finding may contribute to the development of new therapeutic strategies for AD.

15.
Oncogene ; 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33037410

RESUMO

Epigenetic regulations play crucial roles in leukemogenesis and leukemia progression. SUV39H1 is the dominant H3K9 methyltransferase in the hematopoietic system, and its expression declines with aging. However, the role of SUV39H1 via its-mediated repressive modification H3K9me3 in leukemogenesis/leukemia progression remains to be explored. We found that SUV39H1 was down-regulated in a variety of leukemias, including MLL-r AML, as compared with normal individuals. Decreased levels of Suv39h1 expression and genomic H3K9me3 occupancy were observed in LSCs from MLL-r-induced AML mouse models in comparison with that of hematopoietic stem/progenitor cells. Suv39h1 overexpression increased leukemia latency and decreased the frequency of LSCs in MLL-r AML mouse models, while Suv39h1 knockdown accelerated disease progression with increased number of LSCs. Increased Suv39h1 expression led to the inactivation of Hoxb13 and Six1, as well as reversion of Hoxa9/Meis1 downstream target genes, which in turn decelerated leukemia progression. Interestingly, Hoxb13 expression is up-regulated in MLL-AF9-induced AML cells, while knockdown of Hoxb13 in MLL-AF9 leukemic cells significantly prolonged the survival of leukemic mice with reduced LSC frequencies. Our data revealed that SUV39H1 functions as a tumor suppressor in MLL-AF9-induced AML progression. These findings provide the direct link of SUV39H1 to AML development and progression.

16.
Int J Biochem Cell Biol ; 129: 105863, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33049375

RESUMO

INTRODUCTION: Tanshinone IIA (TSIIA), an active component of Salvia miltiorrhiza (Danshen), is reported to inhibit cell proliferation in hypertrophic scars (HS). In our previous study, we observed that lncRNA human-specific regulatory loci (HSRL) was up-regulated in HS tissues. However, whether TSIIA serves as an effective treatment for HS through affecting HSRL is still unexplored. METHODS: TGF-ß1-stimulated fibroblast were used as the in vitro HS model. The effects of TSIIA on cell proliferation were evaluated using CCK-8, Edu staining and colony formation assays. By performing loss-of-function and rescue experiments, we explored the role of HSRL and Sorting nexin 9 (SNX9) in TGF-ß1-stimulated fibroblast. Employing RNA-protein pull-down assay and Co-immunoprecipitation, we further investigated the mechanisms through which TSIIA attenuated TGF-ß1-stimulated fibroblast. RESULTS: Our data demonstrated that TSIIA could effectively attenuate TGF-ß1-mediated fibroblast proliferation in a dose-dependent manner. Meanwhile, TSIIA could down-regulate the expression of α-SMA, VEGFA, Collagen 1, HSRL, SNX9 and p-Smad2/3 in TGF-ß1-stimulated HSF. In addition, we found that SNX9 overexpression reversed the effects of HSRL knockdown on TGF-ß1-stimulated HSF. Furthermore, we confirmed that TSIIA treatment weakens the interaction between p-Smad3 and SNX9 in HS models. CONCLUSIONS: Tanshinone IIA down-regulated p-Smad3 signaling to inhibit TGF-ß1-mediated fibroblast proliferation via lncRNA-HSRL/SNX9.

17.
Pharmacol Res ; 161: 105218, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33007418

RESUMO

Endoplasmic reticulum (ER) stress is easily observed in chronic liver disease, which often causes accumulation of unfolded or misfolded proteins in the ER, leading to unfolded protein response (UPR). Regulating protein degradation is an integral part of UPR to relieve ER stress. The major protein degradation system includes the ubiquitin-proteasome system (UPS) and autophagy. All three arms of UPR triggered in response to ER stress can regulate UPS and autophagy. Accumulated misfolded proteins could activate these arms, and then generate various transcription factors to regulate the expression of UPS-related and autophagy-related genes. The protein degradation process regulated by UPR has great significance in many chronic liver diseases, including non-alcoholic fatty liver disease (NAFLD), alcoholic liver disease (ALD), viral hepatitis, liver fibrosis, and hepatocellular carcinoma(HCC). In most instances, the degradation of excessive proteins protects cells with ER stress survival from apoptosis. According to the specific functions of protein degradation in chronic liver disease, choosing to promote or inhibit this process is promising as a potential method for treating chronic liver disease.

18.
J Vet Med Sci ; 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32999131

RESUMO

Pseudorabies virus (PRV) infection leads to severe inflammatory responses and tissue damage, and many natural herbs exhibit protective effects against viral infection by modulating the inflammatory response. An ethyl acetate fraction of flavonoids from Polygonum hydropiper L. (FEA) was prepared through ethanol extraction and ethyl acetate fractional extraction. An inflammatory model was established in RAW264.7 cells with PRV infection to evaluate the anti-inflammatory activity of FEA by measuring cell viability, nitric oxide (NO) production, reactive oxygen species (ROS) release, and mRNA expression of inflammatory factors, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Its functional mechanism was investigated by analyzing the phosphorylation and nuclear translocation of key proteins in the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Our findings indicate that PRV induced inflammatory responses in RAW264.7 cells, and the responses were similar to that in lipopolysaccharide (LPS)-stimulated cells. FEA significantly suppressed NO synthesis and down-regulated both expression and secretion of COX-2, iNOS, and inflammatory cytokines (P<0.05 or P<0.01). FEA also reduced NF-κB p65 translocation into the nucleus and decreased MAPK phosphorylation, indicating that the NF-κB/MAPK signaling pathway may be closely related to the inflammatory response during viral infection. The findings suggested the potential pharmaceutical application of FEA as a natural product that can treat viral infections due to its ability to mitigate inflammatory responses.

19.
Zygote ; : 1-8, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33054867

RESUMO

In higher plants, fertilization induces many structural and physiological changes in the fertilized egg that reflect the transition from the haploid female gamete to the diploid zygote - the first cell of the sporophyte. After fusion of the egg nucleus with the sperm nucleus, many molecular changes occur in the zygote during the process of zygote activation during embryogenesis. The zygote originates from the egg, from which some pre-stored translation initiation factors transfer into the zygote and function during zygote activation. This indicates that the control of zygote activation is pre-set in the egg. After the egg and sperm nuclei fuse, gene expression is activated in the zygote, and paternal and maternal gene expression patterns are displayed. This highlights the diversity of zygotic genome activation in higher plants. In addition to new gene expression in the zygote, some genes show quantitative changes in expression. The asymmetrical division of the zygote produces an apical cell and a basal cell that have different destinies during plant reconstruction; these destinies are determined in the zygote. This review describes significant advances in research on the mechanisms controlling zygote activation in higher plants.

20.
Int J Oral Implantol (Berl) ; 13(3): 269-277, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32879931

RESUMO

PURPOSE: To compare the short-term clinical and radiographic outcomes of angulated screw-retained and cemented implant crowns following flapless immediate implant placement. MATERIALS AND METHODS: The study was designed as a prospective cohort study with 1-year follow-up. Eligible patients were divided into two groups according to restoration type: the angulated screw group (AG) and the cemented group (CG). Implant survival, marginal bone loss, mechanical complications, probing depth, bleeding on probing% and pink aesthetic score were evaluated. RESULTS: After 1 year of loading, the implant survival rate was 100% in both groups (AG, n = 23; CG, n = 20). A significantly lower bleeding on probing% was found in the AG than in the CG (11.6 ± 19.1% vs. 33.3 ± 33.8%, P = 0.04). No significant differences in marginal bone loss, probing depth and mechanical complication rates were found between the two groups (P = 0.53, 0.48, 0.41, respectively). The overall pink aesthetic score value was 8.96 ± 0.88 in the AG and 8.98 ± 0.62 in the CG at 1-year examination (P = 0.96). The percentage of excellent pink aesthetic scores (≥ 9) value increased from 48% at baseline to 83% at 1 year in the AG, and from 45% at baseline to 85% at 1 year in the CG. CONCLUSION: Based on the 1-year results, both treatment options provide high implant survival, a stable marginal bone level and excellent aesthetic outcomes in the short term. Angulated screw-retained crowns might benefit the long-term peri-implant conditions.


Assuntos
Implantes Dentários para Um Único Dente , Implantes Dentários , Parafusos Ósseos , Coroas , Estética Dentária , Seguimentos , Humanos , Estudos Prospectivos
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