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1.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(9): 873-879, 2021 Sep 09.
Artigo em Chinês | MEDLINE | ID: mdl-34496535

RESUMO

Objective: To record occlusal contact of primary dentition at maximal intercuspal position in children with individual normal occlusion. Methods: A total of 57 children were recruited from patients of the Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology. Inclusion criteria were that the subjects were 3-5 years old with no visually detectable caries or pupal and periapical diseases, had complete primary dentition, had individual normal occlusion, had normal function of craniofacial system, were medically healthy, could cooperate with sampling and had obtained written informed consent from the parents or guardians. Finally, forty-seven children aged 3 to 5 years old were enrolled, including 24 males and 23 females. The age, height and weight of all subjects were (4.1±0.7) years old (ranging 3.0-5.8 years old), (103.7±7.2) cm (ranging 90-120 cm) and (17.1±2.5) kg (ranging 12.5-22.5 kg), respectively. Occlusal abilities such as occlusal contact area, average bite pressure, maximum bite pressure, maximum bite force and occlusal balance were measured with Dental Prescale Ⅱ system. Results: Maximum bite force and occlusal contact area at intercuspal position in children with primary dentition were (567.40±223.84) N (ranging 226.7-1 154.6 N) and (18.56±6.54) mm2 (ranging 8.4-41.2 mm2), respectively. There was a significantly strong correlation between maximum bite force and occlusal contact area (r=0.954, P<0.01). Height and weight of children were also positively correlated with their maximum bite force (r=0.397, P=0.022 and r=0.453, P=0.008, respectively). Maximum occlusal bite force and contact occlusal area of boys [(651.80±224.34) N and (20.77±6.97) mm2] were significantly higher and larger than those of girls [(479.34±190.45) N and (16.25±5.27) mm2] (P<0.05). Thirty-two of all 47 children had one occlusal contact point with maximum bite pressure, mostly locating within the primary molar region. Bite forces of anterior and posterior teeth of primary dentition were (124.12±56.99) N and (450.11±205.09) N, respectively, about (21.82±11.40)% and (71.80±21.35)% of maximum bite force of the whole primary dentition. All of the occlusal balance points located in posterior teeth regions. Occlusal contacts were observed at both anterior and posterior teeth of primary dentition with individual normal occlusion. Conclusions: There was a great variation of maximum bite forces of primary dentitions at intercuspal position of children with individual normal occlusion. Maximum bite force of primary dentition was significantly correlated with occlusal contact area, height and weight of children. Occlusal contact points with maximum bite pressure and occlusal bite balance points of primary dentitions mostly located in primary molar regions.


Assuntos
Força de Mordida , Oclusão Dentária , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Dente Molar , Dente Decíduo
2.
Animal ; 15(1): 100066, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33516033

RESUMO

Crossbreeding is an essential way of improving herd performance. However, frequent parentage record errors appear, which results in the lower accuracy of genetic parameter estimation and genetic evaluation. This study aims to build a single nucleotide polymorphism (SNP) panel with sufficient power for parentage testing in the crossbred population of Simmental and Holstein cattle. The direct sequencing technique in PCR products of pooling DNA along with matrix-assisted laser desorption/ionization time-of-flight MS method for genotyping the individuals was applied. A panel comprising 50 highly informative SNPs for parentage analysis was developed in the crossbred population. The average minor allele frequency for SNPs was 0.43, and the cumulative probability of exclusion for single-parent and both-parent inference met 0.99797 and 0.999999, respectively. The maker-set for parentage verification was then used in a group of 81 trios with aid of the likelihood-based parentage-assignment program of Cervus software. Reconfirmation with on-farm records showed that this 50-SNP system could provide sufficient and reliable information for parentage testing with the parental errors for mother-offspring and sire-offspring being 8.6 and 18.5%, respectively. In conclusion, a set of low-cost and efficient SNPs for the paternity testing in the Simmental and Holstein crossbred population are provided.


Assuntos
Polimorfismo de Nucleotídeo Único , Software , Animais , Biomarcadores , Bovinos/genética , Frequência do Gene , Genótipo , Funções Verossimilhança , Polimorfismo de Nucleotídeo Único/genética
3.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(6): 590-596, 2019 Jun 06.
Artigo em Chinês | MEDLINE | ID: mdl-31177756

RESUMO

Objective: To investigate the relationship of plasma albumin and hypersensitive C-reactive protein (Hs-CRP) with 5-year all-cause mortality among Chinese older adults aged 65 and older. Method: Data was collected in 8 longevity areas of the Chinese Longitudinal Healthy Longevity Survey (CLHLS) study conducted by Chinese Center for Disease Control and Prevention and Peking University at baseline survey in 2012 and 2014, the participants enrolled in 2012 was followed-up in 2014 and 2017, the participants enrolled in 2014 was followed-up in 2017 only. Finally, 3 118 older adults aged 65 and older with complete information on albumin, Hs-CRP and body mass index (BMI) were included in this study. Plasma samples of older adults were collected for the detection of albumin and Hs-CRP at baseline survey. Survival status and follow-up time was recorded for all participants. All older adults were divided into 4 groups according to the levels of plasma albumin and Hs-CRP, and Cox proportional hazard models were constructed to assess their influence on the risk of all-cause mortality. Results: Among 3 118 older adults included, the prevalence of hypoalbuminemia was 10.1% (316/3 118), and was 22.8% (711/3 118) for elevated Hs-CRP. During 10 132 person-years of follow-up, 1 212 participants died. Participants with hypoalbuminemia had increased risk of all-cause mortality, with an hazard ratio (HR) and 95% confidential interval (CI) of 1.18 (1.01-1.38), compared to participants with normal plasma albuminemia; participants with elevated Hs-CRP had increased risk of all-cause mortality, with an HR (95%CI) of 1.18 (1.04-1.35), compared to participants with normal plasma Hs-CRP. Participants with normal plasma albumin and elevated Hs-CRP, with hypoalbuminemia and normal Hs-CRP, with hypoalbuminemia and elevated Hs-CRP also had increased risk of all-cause mortality when compared to those with normal plasma albumin and normal Hs-CRP, the HR (95%CI) were 1.16 (1.01-1.34), 1.11 (0.91-1.37) and 1.43 (1.11-1.83), respectively. Conclusion: Hypoalbuminemia and elevated Hs-CRP were responsible for increased risk of 5-year all-cause mortality among Chinese older adults from 8 longevity areas.


Assuntos
Proteína C-Reativa , Longevidade , Mortalidade , Albumina Sérica , Idoso , Biomarcadores/sangue , Proteína C-Reativa/análise , China , Humanos , Mortalidade/tendências , Fatores de Risco , Albumina Sérica/análise
4.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(5): 519-522, 2019 May 06.
Artigo em Chinês | MEDLINE | ID: mdl-31091612

RESUMO

In order to evaluate the intervention effect of the school-based salt reduction model, 28 primary schools were selected in Shandong Province in September 2014 and randomly divided into intervention group (1 361 students, 1 306 parents) and control group (1 364 students,1 340 parents). A series of "small hands and big hands" salt reduction intervention activities were conducted in intervention group for 8 months. After the intervention, the total awareness rate of salt reduction knowledge, the total holding rate of related beliefs and the total reporting rate of related behaviors were 70.65%, 80.30% and 67.03% among students, and 85.66%, 93.77% and 87.93% among parents, in the intervention group, which were higher than those in the control group (37.12%, 66.52% and 50.07% among students; 55.11%, 87.52% and 57.96% among parents) (all P values <0.05). The school-based salt reduction model is effective and feasible.


Assuntos
Dieta Hipossódica/psicologia , Pais/psicologia , Serviços de Saúde Escolar , Sódio na Dieta/administração & dosagem , Estudantes/psicologia , Adulto , Criança , China , Estudos de Viabilidade , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Avaliação de Programas e Projetos de Saúde , Instituições Acadêmicas , Estudantes/estatística & dados numéricos , Inquéritos e Questionários
6.
Zhonghua Yu Fang Yi Xue Za Zhi ; 51(11): 1007-1011, 2017 Nov 06.
Artigo em Chinês | MEDLINE | ID: mdl-29136746

RESUMO

Objective: To assess the status of handgrip strength of elderly population from longevity areas in China, and to analyze the correlative factors of handgrip strength of elderly people. Methods: Data from Chinese Longitudinal Healthy Longevity Survey (CLHLS) in 2012 was used, from which1 967 participants aged ≥60 years old with valid data of grip strength value from 8 Chinese longevity areas were included. Information on demographics characteristic, life style and health status was collected using questionnaires. The handgrip strength of both left and right hands were measured by grip dynamometer. The different characteristics of group of participants with different grip strength were compared and then analyzed by adopting the Cumulative odds Logistic regression model to identify main factors associated with hand grip strength. Results: The P(50) (P(25)-P(50)) of hand grip strength of elderly people from the eight longevity areas was 20 (11-28) kg; The hand grip strength of males was 26 (18-34) kg, which was higher than that of females(14 (9-20) kg) (P<0.001). Cumulative odds Logistic regression model showed that the hand grip strength of females was lower than males, whose ß value (95%CI) was-1.22 (-1.43--1.00). The elderly who was at a higher age, smoking, drinking or with anemia, had a comparatively lower handgrip strength, whose ß (95%CI) value were separately-0.08(-0.09~-0.07),-0.29(-0.56~-0.02),-0.54(-0.80~-0.28), and-0.41(-0.62~-0.20). And the elderly who had a higher boby mass index, drinking tea and outdoor activities, had a comparatively higher handgrip strength, whose ß(95%CI) value were separately 0.28 (0.15-0.40), 0.25(0.03-0.47) and 0.51(0.30-0.71). Conclusion: Age and gender were the main correlative factors, lifestyles and physical conditions might also be correlative factors of hand grip strength of the elderly from longevity areas in China.


Assuntos
Força da Mão , Longevidade , Idoso , Anemia , China , Feminino , Humanos , Estilo de Vida , Modelos Logísticos , Estudos Longitudinais , Masculino , Inquéritos e Questionários
7.
Zhonghua Yu Fang Yi Xue Za Zhi ; 51(11): 1012-1018, 2017 Nov 06.
Artigo em Chinês | MEDLINE | ID: mdl-29136747

RESUMO

Objective: To explore the association between biomarkers and activities of daily living (ADL) in the elderly over 65 years old from longevity areas in China. Methods: A total of 2 439 people from 8 longevity areas were included in our baseline survey in 2012. Using questionnaires, body measurements, and blood biochemical examinations, information on demographics characteristic, life style, ADL, blood pressure and biomarkers were collected. Based on these six items of ADL (bathing, dressing, indoor activities, toileting, eating, bowel and bladder control), we constructed a dichotomous indicator for ADL. A respondent was defined as ADL disabled if any difficulty in one or more of the above six activities was reported. Information were collected in the follow-up in 2014 using the same questionnaires and examinations. We excluded information on the elderly who lacked ADL or biomarkers test results or with ADL disability at baseline study. Finally 938 elderly people over 65 years old were included in this analysis. Multivariate logistic regression model was used to analyze the influence factors of ADL disability. Results: During the 2-year follow-up, 100 (10.7%) participants developed into ADL disability, with a rate at 10.7%. Multivariate logistic regression analysis indicated that each year increase in age or each 1 mmHg (1 mmHg=0.133 kPa) increase in systolic blood pressure (SBP) would cause the risk of ADL disability to increase 9% or 1%, whose OR (95%CI) were separately 1.09 (1.06-1.12), 1.01 (1.00-1.02). Han nationality or cognitive impairment increased the risk of ADL disability, whose OR (95%CI) values were separately 4.90 (1.13-21.24), 2.47 (1.44-4.25), while increased lymphocyte count (>1.60×10(9)/L), being married, or participating in recreational activities decreased the risk of ADL disability, whose OR (95%CI) values were separately 0.51 (0.31-0.82), 0.52 (0.28-0.96), 0.43 (0.23-0.80). Conclusion: In the elderly elevated lymphocyte count was associated with lower risk of ADL disability. In addition, incresed age, increased SBP, unmarried, Han nationality or cognitive impairment were associated with the increasing risk of ADL disability in older people, while participating in recreational activities would reduce the risk.


Assuntos
Atividades Cotidianas , Pessoas com Deficiência , Longevidade , Idoso , Idoso de 80 Anos ou mais , Biomarcadores , China , Feminino , Humanos , Estilo de Vida , Modelos Logísticos , Masculino , Inquéritos e Questionários
8.
Zhonghua Yu Fang Yi Xue Za Zhi ; 51(11): 1019-1023, 2017 Nov 06.
Artigo em Chinês | MEDLINE | ID: mdl-29136748

RESUMO

Objective: To discuss the relationship between body mass index (BMI) and cognitive impairment among elderly adults aged ≥65 years old from longevity areas of China. Methods: A total of 2 439 elderly adults from 8 longevity areas were included in our baseline survey by answering questionnaire and taking body measurements and blood biochemical examinations in 2012. In the follow-up study in 2014, we studied the cognitive impairment status among the 1 135 elderly adults aging ≥65 years old. Multivariate logistic regression model was used to analyze the relationship between BMI and cognitive impairment risk. Results: 113 participants were defined as cognitive impairment, with a positive rate at 10.0%. The positive rate in group of underweight, normal weight, and overweight and obese elderly were 22.2% (51/230), 7.5% (47/629), 5.4% (15/276). The rate of cognitive impairment in groups of low BMI (<19.7 kg/m(2)), middle BMI (19.7-23.1 kg/m(2)) and high BMI (>23.1 kg/m(2)) were separately 17.7% (66/372), 7.1% (27/379) and 5.2% (20/384) (P<0.001). Every 1.0 kg/m(2) increase in BMI, the risk of cognitive impairment would significantly decreased by 7% (RR(95%CI): 0.93(0.87-0.99)). Compared with participants with middle BMI level, the group of low BMI participants was associated with a significantly increased risk of cognitive impairment (RR (95% CI): 1.98(1.14-3.44)); while there was no significant association was observed in the high BMI group (RR(95%CI): 1.15(0.57-2.32)). Compared with elderly in normal weight, the elderly with under weight was in higher risk of cognitive impairment (RR (95%CI: 2.19(1.31-3.66)) and there was no significant association found between the elderly with overweight (RR(95%CI): 1.18(0.58-2.38)). Conclusion: Among the elderly ≥65 years old from longevity area, low BMI level and underweight were associated with the increased risk of cognitive impairment.


Assuntos
Índice de Massa Corporal , Disfunção Cognitiva , Longevidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Peso Corporal , China , Feminino , Seguimentos , Humanos , Modelos Logísticos , Masculino , Obesidade , Sobrepeso , Fatores de Risco , Inquéritos e Questionários , Magreza
9.
Zhonghua Yu Fang Yi Xue Za Zhi ; 51(11): 1024-1027, 2017 Nov 06.
Artigo em Chinês | MEDLINE | ID: mdl-29136749

RESUMO

Objective: To explore the relationship between successful aging (SA) index and the survival status among elderly from longevity areas of China. Methods: A total of 2 296 old people aged ≥65 years old from 8 longevity areas were enrolled in our baseline survey in 2012. The information of demographics characteristic, life style, self-assessed health and mood or emotional state, cognitive function, abilities of daily living and physical activity were collected by questionnaire. There were 891 SA people in total, whose SA score was 4-5. 891 SA and 1 396 non-SA participated in the follow-up study in 2014. Finally, 860 SA and non-SA people were matched by orientation analysis. A Cox proportional hazards model was used to explore the relationship between successful aging index and survival status. Results: After 2 years of follow-up, a total of 1 442 elderly survived, with an average baseline age at (83.69±10.81) years old and 278 elderly people died, with an average baseline age at (93.41±9.05) years old. The mortality rate was 16.16% (278/1 720) in total, and it was 13.14% (113/860) in SA group, which was lower than it in non-SA group (19.19%, 165/860), and the difference was statistically significant (P=0.001). The analysis of the multivariate Cox regression showed that SA was the protective factor of the survival of the elderly after age and sex adjusted. Compared to the non-SA group, the risk of mortality rate in SA group decreased by 38%, the HR (95% CI) was 0.62 (0.49-0.79). Conclusion: The mortality rate in the SA group was low in the longevity areas in China; compared with non-SA group, the death rate in SA group reduced by 38%.


Assuntos
Envelhecimento Saudável , Longevidade , Idoso , Idoso de 80 Anos ou mais , China , Cognição , Exercício Físico , Feminino , Seguimentos , Humanos , Estilo de Vida , Masculino , Modelos de Riscos Proporcionais , Risco , Inquéritos e Questionários
10.
Zhonghua Yu Fang Yi Xue Za Zhi ; 51(11): 1028-1032, 2017 Nov 06.
Artigo em Chinês | MEDLINE | ID: mdl-29136750

RESUMO

Objective: To investigate the influence factors of survival outcome among elderly aged ≥80 years old. Methods: In baseline survey in 2009, 930 participants aged ≥80 years old were enrolled from 7 longevity areas, to collect the information of socioeconomic factors, life style, cognitive function, activities of daily living and diseases, as well as physical examination to test biomarkers of blood and urine. The survival status was followed up at 2012 and 2014 survey. Stepwise Cox proportional hazards models were used to screen influence factors of 5-year survival. Results: During 5 years of follow-up, 571 participants died, 133 participants were lost to follow up, and the all-cause mortality was 63.4%. In stepwise Cox proportional hazards models, male, unmarried, self-reported poor life quality, disability in daily life, cognitive impairment, cardiovascular and cerebrovascular diseases, chronic kidney diseases were risk factors for elderly survival outcome, with the HR (95%CI) at 1.75 (1.40-2.12), 1.49 (1.10-2.03), 1.40 (1.16-1.69), 1.37 (1.11-1.70), 1.51 (1.22-1.88), 1.62 (1.18-2.23) and 1.48 (1.23-1.77) respectively. Each 1 year increase in age corresponded to 4% increase in mortality risk (HR (95%CI)=1.04 (1.02-1.05)); each 1 kg/m(2) increase in BMI corresponded to 5% increase in mortality risk (HR (95%CI)=0.95 (0.93-0.98)); each 1.0×10(9)/L increase in total lymphocyte count (TLC) corresponded to 13% increase in mortality risk (HR (95%CI)=0.87 (0.76-0.99)). Additionally, the mortality risk decreased 19% (HR (95%CI)=0.81 (0.66-0.98)) in participants with regularly physical exercise compared to those without; and the mortality risk decreased 41% (HR (95% CI)=0.59 (0.40-0.88)) in participants with elevated triglycerides (TG, ≥2.26 mmol/L) compared to those without. Conclusion: In Chinese longevity areas, some nutritional and immune indices such as relatively higher level of BMI, TLC and TG were independent protective factors for 5-year survival outcome, which was different from general adults and younger elderly.


Assuntos
Atividades Cotidianas , Longevidade , Idoso de 80 Anos ou mais , Biomarcadores , China , Cognição , Estudos de Coortes , Exercício Físico , Feminino , Humanos , Estilo de Vida , Masculino , Modelos de Riscos Proporcionais , Fatores de Risco , Fatores Socioeconômicos , Inquéritos e Questionários
11.
J Eur Acad Dermatol Venereol ; 31(2): 337-340, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27538097

RESUMO

BACKGROUND: The 308-nm excimer laser had been proved to be a time-efficient and potent therapeutic alternative for the management of vitiligo. Different results had been reported in different ethnic populations. OBJECTIVE: To investigate the efficacy and related contributing factors of 308-nm excimer laser in Chinese vitiligo patients. METHODS: A total of 979 Chinese patients (3478 lesions) with progressive-stage vitiligo who had received 308-nm excimer laser treatment were recruited from the vitiligo clinic of Shandong Provincial Hospital of Dermatology &Venereology from 2012 to 2014. Efficacy of treatment was evaluated at the end of session by two independent dermatologists based on the before and after images taken. Repigmentation was graded on a 4-point scale: grade 1, poor repigmentation (0-25%); grade 2, moderate repigmentation (26-50%); grade 3, good repigmentation (51-75%); grade 4, excellent repigmentation (76-100%). RESULTS: The mean grade of repigmentation was 2.29, 44.22% showed less than 25% repigmentation, 16.27% showed 26-50% repigmentation, 5.95% showed 51-75% repigmentation and 33.55% showed more than 76% repigmentation. The repigmentation of facial lesions was better than lesions located elsewhere (P < 0.0001), the best response was noted in the periorbital region, while lesions on hands and feet showed poor repigmentation (P < 0.0001). The degree of repigmentation was negatively correlated with disease duration (r = -0.268, P < 0.001), age (r = -0.095, P < 0.001) and shape of lesions (r = -0.114, P < 0.001), whereas it was positively correlated with treatment frequency (r = 0.270, P < 0.001). Lesions with concurrent poliosis were more likely resistant to treatments. CONCLUSION: 308-nm excimer laser appears to be an effective and safe treatment in Chinese vitiligo patients. The clinical response and treatment efficacy was affected by many factors such as age, affected anatomical area, shape of the lesion, disease duration and treatment frequencies.


Assuntos
Terapia a Laser , Vitiligo/cirurgia , Adulto , China , Feminino , Seguimentos , Humanos , Masculino , Estudos Retrospectivos , Adulto Jovem
12.
Zhonghua Yi Xue Za Zhi ; 96(42): 3412-3416, 2016 Nov 15.
Artigo em Chinês | MEDLINE | ID: mdl-27866536

RESUMO

Objective: To investigate the feasibility of the remote control endovascular robotic system in interventional angiography. Method: The endovascular robotic system Heifetz™ and the matched steerable catheter Mirage™ were used to cannulate all the target vessels in the vascular model and cerebral arteries originated from the aorta of Bama mini-pigs under fluoroscopic guidance. The technical success rate, cannulation time and exposure dose were all collected and compared with the result of using conventional techniques. Result: All the target vessels were successfully cannulated.The average cannulation time for the steerable catheter into left subclavian artery, left common carotid artery, brachiocephalic artery, right common carotid artery and right subclavian artery in the vascular model was (21.3±2.8)s, (28.8±5.2)s, (17.7±2.6)s, (31.5±5.1)s and (24.2±3.7) s, respectively, while the average exposure dose was (9.3±1.2)mGy, (12.4±2.2)mGy, (7.4±1.2)mGy, (14.2±2.5)mGy and( 10.4±1.9)mGy, respectively. The endovascular robotic system completed the cerebral angiography in Bama mini-pigs successfully. The average cannulation time for left innominate artery, right innominate artery, right subclavian artery, common internal carotid trunk, left internal carotid artery and right internal carotid artery was (41.5±6.8)s, (29.1±3.7)s, (40.7±5.5)s, (40.1±5.8)s, (59.6±9.0)s and( 60.3±10.1)s, respectively, while the average exposure dose was (40.6±6.5)mGy, (36.0±5.2)mGy, (39.8±6.1)mGy, (43.9±6.7)mGy, (51.0±7.4)mGy and( 50.1±7.8)mGy, respectively. There was no significant difference between robotic and conventional group in success rate, cannulation time and exposure dose. Conclusion: The remote digital control endovascular robotic system could cannulate the target vessel in both vascular model and complete the cerebral angiography in Bama mini-pigs, which shows the feasibility of using this robotic system in endovascular intervention procedures under remote control.


Assuntos
Procedimentos Endovasculares , Procedimentos Cirúrgicos Robóticos , Aorta Torácica , Artéria Carótida Primitiva , Cateterismo , Angiografia Cerebral , Fluoroscopia , Humanos , Masculino , Robótica , Artéria Subclávia
13.
Zhonghua Yu Fang Yi Xue Za Zhi ; 50(9): 836-840, 2016 Sep 06.
Artigo em Chinês | MEDLINE | ID: mdl-27655609

RESUMO

Established models of type 2 diabetes mellitus(T2DM)are used to determine predictors with the aim of disease prevention. Established models are used to determine predictors, then assign risk scores or calculate the probability of T2DM development within a certain timeframe. A number of countries and regions have established T2DM risk assessment models, which can be divided into non-invasive and invasive tools, depending on whether they use routinely collected information or laboratory markers. Here, we review the latest progress of two assessment models at the national and international levels, and explore and summarize their applications. The noninvasive Finnish Diabetes Risk Score and the invasive Framingham model are widely used internationally. However, invasive models were more widely applied, as studies on T2DM risk assessment models started relatively late in China.


Assuntos
Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/etnologia , Medição de Risco , Grupo com Ancestrais do Continente Asiático , Biomarcadores , China , Diabetes Mellitus Tipo 2/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Pesquisa/tendências , Fatores de Risco , Sensibilidade e Especificidade
14.
Zhonghua Yu Fang Yi Xue Za Zhi ; 50(5): 397-403, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-27141894

RESUMO

OBJECTIVE: To provide a noninvasive type 2 diabetes mellitus (T2DM) prediction model for a rural Chinese population. METHODS: From July to August, 2007 and July to August, 2008, a total of 20 194 participants aged ≥18 years were selected by cluster sampling technique from a rural population in two townships of Henan province, China. Data were collected by questionnaire interview, anthropometric measurement, and fasting plasma glucose and lipid profile examination. A total 17 265 participants were followed up from July to August, 2013 and July to October, 2014. Finally, 12 285 participants were selected for analysis. Data for these participants were randomly divided into a derivation group (derivation dataset, n= 6 143) and validation group (validation dataset, n=6 142) by 1∶1, respectively. Randomization was carried out by the use of computer-generated random numbers. A Cox regression model was used to analyze risk factors of T2DM in the derivation dataset. A T2DM prediction model was established by multiplying ß by 10 for each significant variable. After the total score was calculated by the model, analysis of the receiver operating characteristic (ROC) curve was performed. The area under the ROC curve (AUC) was used for evaluating model predictability. Furthermore, the model's predictability was validated in the validation dataset and compared with the Finnish Diabetes Risk Score (FINDRISC) model. RESULTS: A total 779 of 12 285 participants developed T2DM during the 6-year study period. The incidence rate was 6.12% in the derivation dataset (n=376) and 6.56% in the validation dataset (n=403). The difference was not statistically significant (χ(2)=1.00, P=0.316). A total of four noninvasive T2DM prediction models were established using the Cox regression model. The ROCs of the risk score calculated by the prediction models indicated that the AUCs of these models were similar (0.67-0.70). The AUC and Youden index of model 4 was the highest. The optimal cut-off value, sensitivity, specificity, and Youden index were scores of 25, 65.96%, 66.47%, and 0.32, respectively. Age, sleep time, BMI, waist circumference, and hypertension were selected as predictive variables. Using age<30 years as reference, ß values were 1.07, 1.58, and 1.67 and assigned scores were 11, 16, and 17 for age groups 30-44, 45-59, and ≥60 years, respectively. Using sleep time<8.0 h/d as reference, the ß value and assigned score were 0.27 and 3, respectively, for sleep time ≥10.0 h/d. Using BMI 18.5-23.9 kg/m(2) as reference, ß values were 0.53 and 1.00 and assigned scores 5 and 10, respectively, for BMI 24.0-27.9 kg/m(2), and ≥28.0 kg/m(2). Using waist circumference <85 cm for males/< 80 cm for females as reference, ß values were 0.44 and 0.65 and assigned scores 4 and 7, respectively, for 85 cm ≤ waist circumference <90 cm for males/80 cm≤ waist circumference <85 cm for females, and waist circumference ≥90 cm for males/≥85 cm for females. Using nonhypertension as reference, the respective ß value and assigned score were 0.34 and 3 for hypertension. The AUC performance of this model and the FINDRISC model was 0.66 and 0.64 (P=0.135), respectively, in the validation dataset. CONCLUSION: Based on this cohort study, a noninvasive prediction model that included age, sleep time, BMI, waist circumference, and hypertension was established, which is equivalent to the FINDRISC model and applicable to a rural Chinese population.


Assuntos
Grupo com Ancestrais do Continente Asiático , Índice de Massa Corporal , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/etnologia , População Rural/estatística & dados numéricos , Adulto , China , Estudos de Coortes , Diabetes Mellitus Tipo 2/epidemiologia , Feminino , Humanos , Hipertensão/epidemiologia , Hipertensão/etnologia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Curva ROC , Fatores de Risco , Sensibilidade e Especificidade , Circunferência da Cintura
15.
Genet Mol Res ; 14(4): 14893-9, 2015 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-26600550

RESUMO

The purpose of this study was to screen for genes that were differentially expressed between a human gastric carcinoma cell line (HGC-27) and their tumor spheres, using the gene chip technique. The HGC-27 cells and tumor sphere cells were cultured in vitro in a sterile environment. Total RNA was extracted from both samples and purified using a standard TRIzol reagent. Total RNA was then hybridized onto a GeneChip, according to the standard protocols provided by the manufacturers of the GeneChip IVT Express Kit. The resulting fluorescence signals were analyzed and displayed using the Cluster and Treeview software programs. Under the criteria for significant differential expression (≥2-fold difference), 610 up- and 1135 down-regulated genes were identified in tumor sphere cells, compared to HCG-27 cells. These genes were involved in cell growth, signal transduction, tumorigenesis, and many other functional aspects of tumor cells. In conclusion, a number of genes were differentially expressed in tumor sphere cells compared to HCG-27 cells. In addition, we identified a close correlation between tumor sphere cells and tumorigenesis.


Assuntos
Carcinogênese/genética , Regulação Neoplásica da Expressão Gênica , Proteínas de Neoplasias/biossíntese , Neoplasias Gástricas/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Humanos , Proteínas de Neoplasias/genética , Análise de Sequência com Séries de Oligonucleotídeos , Esferoides Celulares/metabolismo , Neoplasias Gástricas/patologia
16.
Genet Mol Res ; 11(3): 2569-77, 2012 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-22843074

RESUMO

DNA methylation is essential for adipose deposition in mammals. We screened SNPs of the bovine DNA methyltransferase 3b (DNMT3b) gene in Snow Dragon beef, a commercial beef cattle population in China. Nine SNPs were found in the population and three of six novel SNPs were chosen for genotyping and analyzing a possible association with 16 meat quality traits. The frequencies of the alleles and genotypes of the three SNPs in Snow Dragon beef were similar to those in their terminal-paternal breed, Wagyu. Association analysis disclosed that SNP1 was not associated with any of the traits; SNP2 was significantly associated with lean meat color score and chuck short rib score, and SNP3 had a significant effect on dressing percentage and back-fat thickness in the beef population. The individuals with genotype GG for SNP2 had a 25.7% increase in lean meat color score and a 146% increase in chuck short rib score, compared with genotype AA. The cattle with genotype AG for SNP3 had 35.7 and 24% increases in dressing percentage and 28.8 and 29.2% increases in back-fat thickness, compared with genotypes GG and AA, respectively. Genotypic combination analysis revealed significant interactions between SNP1 and SNP2 and between SNP2 and SNP3 for the traits rib-eye area and live weight. We conclude that there is considerable evidence that DNMT3b is a determiner of beef quality traits.


Assuntos
Bovinos/genética , DNA (Citosina-5-)-Metiltransferases/genética , Estudos de Associação Genética , Carne , Polimorfismo de Nucleotídeo Único/genética , Característica Quantitativa Herdável , Animais , Sequência de Bases , Cruzamento , Frequência do Gene/genética , Genótipo , Dados de Sequência Molecular
17.
J Dairy Sci ; 94(11): 5695-8, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22032394

RESUMO

In dairy cattle, 4 important recessive hereditary diseases exist: complex vertebral malformation (CVM), bovine leukocyte adhesion deficiency (BLAD), citrullinemia (CTLN), and deficiency of uridine monophosphate synthase (DUMPS). Holstein Associations in developed countries have established monitoring systems for such disorders in Holstein bulls for decades. Over the past decades, China has continuously imported Holstein semen and embryos, mainly from North America but also from Europe. The dissemination of such genetic defects was undetermined until now, although efforts were taken to develop molecular techniques and detect carriers for CVM and BLAD in small populations of Chinese dairy cattle. Thus, herein we extensively screened 732 proven bulls participating in artificial insemination programs and 136 young bulls entering progeny test from 15 bull stations in China for CVM, BLAD, CTLN, and DUMPS. The proportion of carriers of the defects was found to be 7.72, 1.38, 0.23, and 0.12%, respectively. Given our findings, early diagnostic and monitoring systems on recessive inherited disorders among proven and young bulls entering the national genetic improvement programs for dairy cattle of China should be established immediately, in which a series of measures will be taken to prevent further spreading of such disorders and gradually eliminate them in the dairy cattle population in China.


Assuntos
Doenças dos Bovinos/genética , Genes Recessivos/genética , Heterozigoto , Animais , Cruzamento , Bovinos , Doenças dos Bovinos/epidemiologia , China , Indústria de Laticínios , Testes Genéticos/veterinária , Masculino
18.
Eur J Vasc Endovasc Surg ; 41(2): 288-93, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21123096

RESUMO

BACKGROUND AND OBJECTIVE: Zoledronate has been reported to inhibit the proliferation, adhesion and migration of vascular smooth muscle cells. In the present study, we assessed whether systemic and local delivery of zoledronate would be sufficient to prevent intimal hyperplasia. METHODS: Twenty-four male Sprague-Dawley rats were assigned into four groups: non-treated group, systemic zoledronate-treated group, local collagen-treated group and local zoledronate-treated group. All four groups underwent balloon injury to the right common carotid artery. The left uninjured carotid arteries of the non-treated group were considered as normal artery samples. Twenty-one days after arterial injury and treatment, the right and left common carotid arteries were fixed, sectioned, stained and measured by computer-aided image analysis. RESULTS: At 3 weeks, there was a 59% reduction of the intima/media area ratio in the systemic zoledronate-treated group compared with the non-treated group (P < 0.01). There was an 87% reduction of the intima/media area ratio in the local zoledronate-treated group compared with the local collagen-treated group (P < 0.01). CONCLUSIONS: Both systemic and local delivery of zoledronate correspond to a significant reduction in intimal hyperplasia seen at 3 weeks.


Assuntos
Angioplastia com Balão/efeitos adversos , Fármacos Cardiovasculares/administração & dosagem , Lesões das Artérias Carótidas/tratamento farmacológico , Artéria Carótida Primitiva/efeitos dos fármacos , Difosfonatos/administração & dosagem , Imidazóis/administração & dosagem , Túnica Íntima/efeitos dos fármacos , Administração Tópica , Animais , Lesões das Artérias Carótidas/etiologia , Lesões das Artérias Carótidas/patologia , Artéria Carótida Primitiva/patologia , Proliferação de Células/efeitos dos fármacos , Modelos Animais de Doenças , Hiperplasia , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Injeções Intravenosas , Masculino , Antígeno Nuclear de Célula em Proliferação/metabolismo , Ratos , Ratos Sprague-Dawley , Fatores de Tempo , Túnica Íntima/lesões , Túnica Íntima/patologia , Ácido Zoledrônico
19.
Zhongguo Zhong Yao Za Zhi ; 25(7): 419-21, 2000 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-12515223

RESUMO

OBJECTIVE: To investigate new compounds with anti-tumor activities from the root of Desmos grandifolius of Annonaceae. METHOD: The chemical constituents were isolated by silica gel column chromatography, and the structures were elucidated by UV, IR, MS, 1H NMR, 13C NMR, H-H COSY and NOESY analysis. RESULTS: Six compounds have been isolated from the CHCl3 extract of this plant and identified as lawinal(I), isolawinal(II), desmethoxymatteucinol(III), benzoic acid(IV), beta-sitosterol(V) and sitgmasterol (VI). CONCLUSION: All compounds were found from this plant for the first time. Three dihydroflavonoids isolated from this species are characterized by A-ring substitution and no B-ring substitution.


Assuntos
Annonaceae/química , Ácido Benzoico/isolamento & purificação , Flavonoides/isolamento & purificação , Plantas Medicinais/química , Ácido Benzoico/química , Flavonoides/química , Raízes de Plantas/química , Sitosteroides/química , Sitosteroides/isolamento & purificação , Estigmasterol/química , Estigmasterol/isolamento & purificação
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