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1.
Zhongguo Zhen Jiu ; 39(9): 936-9, 2019 Sep 12.
Artigo em Chinês | MEDLINE | ID: mdl-31544380

RESUMO

OBJECTIVE: To compared the therapeutic effect between filiform fire needle assisted 308 nm excimer laser and simple 308 nm excimer laser on vitiligo of different parts. METHODS: Target lesions of 134 patients were divided into an observation group and a control group according to the principle of self-controlled, 201 pieces in each one. In the observation group, filiform fire needle was performed at target lesions. Then target lesions both of the two groups were irradiated with 308 nm excimer laser at the same time. Once every 2 weeks, totally 10 treatments were required. The effective rate and effective rate, color recovery rate and responding time of different parts in the two groups were evaluated 2 weeks after treatment. RESULTS: The effective rate in the observation group was 82.59% (166/201), which was higher than 68.16% (137/201) in the control group (P<0.01). The effective rate of face-neck, trunk, limbs and hand-foot were 90.32%, 81.63%, 81.48% and 58.62% respectively in the observation group, which were higher than 82.80%, 69.39%, 51.85% and 31.03% in the control group (P<0.01, P<0.05). The color recovery rate of different parts in the observation group was higher than the control group, and the effect was faster in the observation group (P<0.01, P<0.05). CONCLUSION: Filiform fire needle as an adjunctive therapy, combined with 308 nm excimer laser are more effective than simple 308 nm excimer laser for vitiligo of different parts. Combination therapy has a shorter responding time, the face-neck has the best effect and hand-foot has poor effect.


Assuntos
Lasers de Excimer , Vitiligo , Terapia Combinada , Humanos , Pescoço , Resultado do Tratamento , Vitiligo/terapia
2.
Huan Jing Ke Xue ; 37(10): 3835-3841, 2016 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964416

RESUMO

In order to study the efficiency and mechanism of capping with purple parent rocks to control phosphorus release from sediments, three kinds of purple parent rocks (Fei-xian-guan Formation, Peng-lai-zhen Formation and Sui-ning Formation) which distribute widely in Chongqing, and two types of rock (limestone and calcite) which have been found to effectively control the release of phosphorus from sediments, were selected as active covering materials to cap the sediments to conduct simulation experiment. The results indicated that: all three kinds of purple parent rocks showed strong inhibition of total phosphorus release from sediments to the overlying water, far better than limestone and calcite (P<0.05), and the highest removal rate of total phosphorus was 94.4% in the disposal of Fei-xian-guan Formation. Those five kinds of covering materials could promote the release and transformation of total phosphorus in sediments, which could significantly promote the in situ conversion of OP to Ca-P, while the three kinds of purple parent rocks were more conducive to convert the released phosphorus into inorganic phosphorus and organic phosphorus. At the same time, those five kinds of covering materials could also change the microbial community structure in sediments and overlying water, and the numbers of bacteria (labeled as PLFA16:0) were significantly negatively correlated with the content of total phosphorus in the overlying water.

3.
Zhonghua Nan Ke Xue ; 21(9): 819-23, 2015 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-26552216

RESUMO

OBJECTIVE: To investigate whether intracytoplasmic sperm injection (ICSI) can improve the clinical outcomes of the male patients with 100% teratozoospermia. METHODS: We retrospectively analyzed the clinical data of 152 couples undergoing in vitro fertilization-embryo transfer (IVF-ET), including 75 cycles of IVF and 77 cycles of ICSI. We compared the rates of normal fertilization, high-quality embryos, transferrable embryos, implantation, clinical pregnancy, and abortion between the two groups. RESULTS: In the 100% teratozoospermia patients the number of transferrable embryos was significantly lower in the IVF than in the ICSI group (78.91% vs 84.92%, P < 0.05), while the rates of normal fertilization and implantation were higher in the former than in the latter (60.26% vs 57.87% and 48.00% vs 39.55%, both P > 0.05). There were no statistically significant differences between the two groups in the female age, Gn days, Gn dose, BMI, infertility duration, endometrial thickness, and basal serum FSH and E2. CONCLUSION: ICSI cannot improve the clinical outcomes of the male patients with 100% teratozoospermia.


Assuntos
Azoospermia , Implantação do Embrião , Transferência Embrionária , Fertilização , Injeções de Esperma Intracitoplásmicas , Aborto Espontâneo , Feminino , Fertilização In Vitro/estatística & dados numéricos , Humanos , Masculino , Gravidez , Estudos Retrospectivos , Injeções de Esperma Intracitoplásmicas/estatística & dados numéricos
4.
Huan Jing Ke Xue ; 36(5): 1639-47, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26314110

RESUMO

In order to understand the impacts of physicochemical properties of purple parent rock on the removal rates of nitrogen, phosphorus and organics in landscape water systems, four types of purple parent rocks including Peng-lai-zhen Formation (S1) , Sha-xi-miao Formation (S2) , Fei-xian-guan Formation (S3) and Sui-ning Formation (S4) , which distribute widely in Chongqing, were selected and autoclaved, and added to unsterile landscape water collected from Chong-de Lake in Southwest University, and the landscape water only was used as control. And several indicators such as total nitrogen and phosphorus and so on of every disposal were investigated periodically. The results indicated that: (1) The highest removal rates of total nitrogen, total phosphorus and Ammonia nitrogen were observed in Sl, which were 45.1%, 62.3% and 90%, respectively; the highest removal rate of COD was 94.5% in S4; the ammonia nitrogen content in the purple parent rocks was not obviously changed before and after the experiments, which indicated that the adsorption of ammonia nitrogen on purple parent rock surface was not the main reason for the decrease of ammonia nitrogen in water. (2) Arsenate had inhibitory effect on the sulfate-reducing bacteria, while copper and magnesium had promoting effect on gram-negative bacteria. (3) The microbial diversity was positively correlated to total nitrogen in water. (4) Based on the PCA analyses of microbial community structure and environmental factors, the mineral elements released from parent rock affected the structure and composition of microbial community in the test water, and then influenced the removal rates of nitrogen, phosphorus and organics in water systems.


Assuntos
Água Doce/química , Nitrogênio/análise , Compostos Orgânicos/análise , Fósforo/análise , Lagos
5.
J Assist Reprod Genet ; 31(9): 1161-6, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25015033

RESUMO

PURPOSE: To compare the efficacy of swim-up and DGC in improving sperm deformity and DNA fragmentation and to determine which method is better in teratozoospermic patients requiring artificial reproduction. METHODS: The present study compared the effects of swim-up and density gradient centrifugation (DGC), the two most commonly used semen preparation methods, on sperm deformity rate and DNA fragmentation index (DFI) in semen samples from teratozoospermic patients. RESULTS: The results demonstrated that both swim-up and DGC yielded a significantly lower sperm deformity rate and DFI in comparison to unprocessed whole semen, with DGC having more favorable results. Sperm deformity rate in unprocessed whole semen samples was significantly lower in the 20-29 age group than in the 40-49 age group, but no significant difference was observed in DFI between different age groups. There was no significant correlation between sperm deformity rate and DFI. CONCLUSIONS: Our findings suggest that enrichment of sperm with normal morphology and intact DNA in teratozoospermic patients could be achieved by both DGC and swim-up procedures, and that DGC is a better method.


Assuntos
Fragmentação do DNA , Infertilidade Masculina/genética , Técnicas de Reprodução Assistida , Análise do Sêmen/métodos , Espermatozoides/anormalidades , Adulto , Fatores Etários , Centrifugação com Gradiente de Concentração , Humanos , Masculino , Pessoa de Meia-Idade
6.
Zhonghua Nan Ke Xue ; 20(6): 523-6, 2014 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-25029858

RESUMO

OBJECTIVE: To investigate the influence of seasons, blood types and semen parameters on the cryosurvival rate of frozen-thawed spermatozoa from sperm donors. METHODS: We retrospectively analyzed the influence of seasons, blood types, abstinence period, semen volume, sperm morphology, and pre-freeze sperm motility and concentration on the cryosurvival rate of frozen-thawed sperm in 4 088 semen samples from Shaanxi Human Sperm Bank. RESULTS: The cryosurvival rate of the post-thaw sperm was correlated negatively with the progressive motility of the pre-freeze sperm (r = -0.262, P < 0.01), but positively with pre-freeze sperm concentration (r = 0.247, P < 0.01), and it was significantly higher in the 6-day abstinence group ([70.2 +/- 5.4]%) than in the other abstinence groups (P < 0.01), so was it in the normal sperm morphology rate > 20% group ([71.4 +/- 5.1]%) than in the others (P < 0.01). The survival rate of the post-thaw sperm was not correlated with seasons, blood types, and semen volume (P > 0.05). CONCLUSION: Sperm motility, concentration and morphology and the abstinence period of sperm donors may be valuable predictors of the cryosurvival rate of post-thaw sperm, but no correlation was found between the survival rate and seasons, blood types and semen volume.


Assuntos
Criopreservação , Preservação do Sêmen , Adulto , Humanos , Masculino , Estudos Retrospectivos , Motilidade Espermática , Espermatozoides , Adulto Jovem
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